Rmu_sc0001183.1_g000001

Acyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001183.1
OG0007995
Physical Location & Seq
Forward (+)
2402 .. 7484
5083 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1338 bp
atgcgtggcggcaaggatcatggcggagcaaccgcggcggcgtgggttgacagcaccgcactggaactgcagcgttggttcatcatccgttggaggtggaagctggtaaccaccaccaccgcaatgggccaggccggcctcaccctcaccgcgccgctcctctctcctcttccgccgtccgatcaaccccacttgatcgtcaccgtccgcgacgacgacggcggcgacggcgacggcaacggcagcttccgcaatccgtttgcgtttctcgggtcgggcgggtttaaggtgcccgtatcgacgacggcggatccgttcaagaaccatactttgaaaatcggaggggtttacgagtggttaaagatcggggtttgcttccccatcgcgattgtgaggcttgtgatctttggggtttcgttgctgattgggtttgtggccaccaaattggcgctgttgggatggaaggagaggcagaaccctatgcccaagtggaggtgtaggattatgtggatcactagggtctgtaccagatgtattctcttcgcctttggttaccattggataagacggataggaaaacctgctccaagagcaaccgccccaattgttgtatctaaccatgtatcttttatagaacctatattctacttttatgaactattccctacaattgtcgcatctgagtcccatgattccctaccttttgttggaaccatcatcagagcaatgcaggtgatatatgttaataggttttcaccatcatcaaggaggcaagctgttactgaaataaagagaaaagcttcatgcgatagatttcctcgagtgctcctatttcctgagggaaccacaaccaacggaagatatcttatttcatttgaacttggtgcattcatccctggttacccaatccaaccagtaattgtacgctatcctcatgtgcactttgatcaatcctgggggcacatttctttgccaaggctcatgtttcgaatgttcacgcaatttcacaatttcatggaggtcgagtaccttcctgttgtttcacctcttgataacaagaaagaaagtgctttccactttgctcagaggactagtcatgctatggcagctgcactcaatgttgtacagacatctcattcttttggagacttgatgcttctcatgaaagcagctgattcaaaatcgaaacaggtgcggccctcagcttacatggttgaaatggcgagtgtaaaatctcgctcctactccttctcctttttcatcgtcgttggtccttttgacataacattctcggccgcctccggtccggtaggaaagaaacctgtagccagtgactag

Protein Analysis

445

Amino Acids

49.77

Weight (kDa)

9.88

Isoelectric Point (pI)

41.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Acyltransferase PF01553 196 - 308 1.9e-14 Acyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 721
Acc36I ACCTGC 2 cut(s) 589, 721
AccB1I GGYRCC 1 cut(s) 289
AccBSI CCGCTC 1 cut(s) 157
AccII CGCG 4 cut(s) 35, 152, 210, 386
AclWI GGATC 4 cut(s) 24, 305, 318, 518
AcoI YGGCCR 2 cut(s) 435, 1293
AfaI GTAC 4 cut(s) 526, 924, 1028, 1125
AfiI CCNNNNNNNGG 2 cut(s) 492, 707
AgsI TTSAA 5 cut(s) 319, 334, 878, 1179, 1217
AhlI ACTAGT 1 cut(s) 1091
AjnI CCWGG 3 cut(s) 129, 895, 953
AluBI AGCT 7 cut(s) 103, 246, 776, 800, 1109, 1172, 1205
AluI AGCT 7 cut(s) 103, 246, 776, 800, 1109, 1172, 1205
Alw21I GWGCWC 2 cut(s) 828, 942
Alw26I GTCTC 1 cut(s) 1140
Alw44I GTGCAC 1 cut(s) 938
AlwI GGATC 4 cut(s) 24, 305, 318, 518
Ama87I CYCGRG 2 cut(s) 269, 819
AoxI GGCC 6 cut(s) 127, 132, 136, 435, 1196, 1293
ApaLI GTGCAC 1 cut(s) 938
ApeKI GCWGC 5 cut(s) 70, 243, 1106, 1109, 1169
Asp700I GAANNNNTTC 1 cut(s) 799
AspLEI GCGC 2 cut(s) 154, 451
AspS9I GGNCC 4 cut(s) 127, 1197, 1271, 1304
AsuHPI GGTGA 6 cut(s) 133, 139, 193, 745, 747, 1035
AsuII TTCGAA 1 cut(s) 988
AvaI CYCGRG 2 cut(s) 269, 819
AvaII GGWCC 2 cut(s) 1271, 1304
AxyI CCTNAGG 1 cut(s) 837
BaeGI GKGCMC 3 cut(s) 294, 942, 963
BalI TGGCCA 1 cut(s) 437
BamHI GGATCC 1 cut(s) 310
BanI GGYRCC 1 cut(s) 289
Bbv12I GWGCWC 2 cut(s) 828, 942
BbvCI CCTCAGC 1 cut(s) 1201
BbvI GCAGC 5 cut(s) 82, 255, 1096, 1118, 1181
BccI CCATC 4 cut(s) 389, 453, 722, 766
BceAI ACGGC 6 cut(s) 160, 235, 244, 250, 256, 321
BcgI CGANNNNNNTGC 2 cut(s) 1174, 1208
BciT130I CCWGG 3 cut(s) 131, 897, 955
BclI TGATCA 1 cut(s) 946
BcoDI GTCTC 1 cut(s) 1140
BcuI ACTAGT 1 cut(s) 1091
BfaI CTAG 3 cut(s) 516, 1092, 1336
BfmI CTRYAG 2 cut(s) 68, 1323
BfoI RGCGCY 1 cut(s) 452
BfuAI ACCTGC 2 cut(s) 589, 721
BglI GCCNNNNNGGC 1 cut(s) 135
Bme1390I CCNGG 3 cut(s) 131, 897, 955
Bme18I GGWCC 2 cut(s) 1271, 1304
BmeT110I CYCGRG 2 cut(s) 269, 819
BmgT120I GGNCC 4 cut(s) 127, 1197, 1271, 1304
BmiI GGNNCC 4 cut(s) 291, 312, 712, 844
BmrFI CCNGG 3 cut(s) 131, 897, 955
BmsI GCATC 2 cut(s) 686, 1143
Bpu10I CCTNAGC 1 cut(s) 1201
Bpu14I TTCGAA 1 cut(s) 988
BsaJI CCNNGG 4 cut(s) 33, 895, 954, 974
BsaWI WCCGGW 2 cut(s) 1301, 1306
BsaXI ACNNNNNCTCC 4 cut(s) 1010, 1040, 1235, 1265
Bsc4I CCNNNNNNNGG 2 cut(s) 492, 707
Bse118I RCCGGY 1 cut(s) 134
Bse1I ACTGG 3 cut(s) 66, 914, 1329
Bse21I CCTNAGG 1 cut(s) 837
Bse3DI GCAATG 2 cut(s) 129, 732
BseBI CCWGG 3 cut(s) 131, 897, 955
BseDI CCNNGG 4 cut(s) 33, 895, 954, 974
BseGI GGATG 3 cut(s) 84, 464, 891
BseLI CCNNNNNNNGG 2 cut(s) 492, 707
BseMI GCAATG 2 cut(s) 129, 732
BseMII CTCAG 4 cut(s) 672, 828, 1097, 1215
BseNI ACTGG 3 cut(s) 66, 914, 1329
BseRI GAGGAG 2 cut(s) 149, 156
BseSI GKGCMC 3 cut(s) 294, 942, 963
BseX3I CGGCCG 1 cut(s) 1293
BseXI GCAGC 5 cut(s) 82, 255, 1096, 1118, 1181
BsgI GTGCAG 1 cut(s) 1095
Bsh1236I CGCG 4 cut(s) 35, 152, 210, 386
Bsh1285I CGRYCG 1 cut(s) 1296
BshFI GGCC 6 cut(s) 129, 134, 138, 437, 1198, 1295
BshNI GGYRCC 1 cut(s) 289
BsiEI CGRYCG 1 cut(s) 1296
BsiHKAI GWGCWC 2 cut(s) 828, 942
BsiHKCI CYCGRG 2 cut(s) 269, 819
BsiSI CCGG 3 cut(s) 135, 1302, 1307
BslFI GGGAC 1 cut(s) 670
BslI CCNNNNNNNGG 2 cut(s) 492, 707
BsmAI GTCTC 1 cut(s) 1140
BsmFI GGGAC 1 cut(s) 670
BsmI GAATGC 1 cut(s) 887
BsnI GGCC 6 cut(s) 129, 134, 138, 437, 1198, 1295
BsoBI CYCGRG 2 cut(s) 269, 819
Bsp119I TTCGAA 1 cut(s) 988
Bsp1286I GDGCHC 4 cut(s) 294, 828, 942, 963
Bsp1407I TGTACA 1 cut(s) 1123
Bsp143I GATC 8 cut(s) 16, 181, 195, 310, 363, 402, 510, 946
Bsp68I TCGCGA 1 cut(s) 386
BspANI GGCC 6 cut(s) 129, 134, 138, 437, 1198, 1295
BspCNI CTCAG 4 cut(s) 673, 829, 1096, 1214
BspFNI CGCG 4 cut(s) 35, 152, 210, 386
BspHI TCATGA 1 cut(s) 1161
BspLI GGNNCC 4 cut(s) 291, 312, 712, 844
BspMAI CTGCAG 1 cut(s) 72
BspMI ACCTGC 2 cut(s) 589, 721
BspPI GGATC 4 cut(s) 24, 305, 318, 518
BspT104I TTCGAA 1 cut(s) 988
BspT107I GGYRCC 1 cut(s) 289
BsrBI CCGCTC 1 cut(s) 157
BsrDI GCAATG 2 cut(s) 129, 732
BsrFI RCCGGY 1 cut(s) 134
BsrGI TGTACA 1 cut(s) 1123
BsrI ACTGG 3 cut(s) 66, 914, 1329
BssAI RCCGGY 1 cut(s) 134
BssECI CCNNGG 4 cut(s) 33, 895, 954, 974
BssMI GATC 8 cut(s) 16, 181, 195, 310, 363, 402, 510, 946
BssT1I CCWWGG 1 cut(s) 974
Bst2UI CCWGG 3 cut(s) 131, 897, 955
Bst4CI ACNGT 1 cut(s) 205
Bst6I CTCTTC 2 cut(s) 174, 545
BstAUI TGTACA 1 cut(s) 1123
BstBI TTCGAA 1 cut(s) 988
BstC8I GCNNGC 2 cut(s) 136, 774
BstDEI CTNAG 4 cut(s) 681, 837, 1083, 1201
BstDSI CCRYGG 1 cut(s) 33
BstEII GGTNACC 3 cut(s) 106, 551, 899
BstF5I GGATG 3 cut(s) 84, 464, 891
BstFNI CGCG 4 cut(s) 35, 152, 210, 386
BstH2I RGCGCY 1 cut(s) 452
BstHHI GCGC 2 cut(s) 154, 451
BstKTI GATC 8 cut(s) 19, 184, 198, 313, 366, 405, 513, 949
BstMAI GTCTC 1 cut(s) 1140
BstMBI GATC 8 cut(s) 16, 181, 195, 310, 363, 402, 510, 946
BstMCI CGRYCG 1 cut(s) 1296
BstMWI GCNNNNNNNGC 7 cut(s) 35, 135, 228, 243, 249, 590, 1106
BstNI CCWGG 3 cut(s) 131, 897, 955
BstPI GGTNACC 3 cut(s) 106, 551, 899
BstSCI CCNGG 3 cut(s) 129, 895, 953
BstSFI CTRYAG 2 cut(s) 68, 1323
BstSLI GKGCMC 3 cut(s) 294, 942, 963
BstUI CGCG 4 cut(s) 35, 152, 210, 386
BstV1I GCAGC 5 cut(s) 82, 255, 1096, 1118, 1181
BstX2I RGATCY 1 cut(s) 310
BstXI CCANNNNNNTGG 2 cut(s) 124, 445
BstYI RGATCY 1 cut(s) 310
BstZI CGGCCG 1 cut(s) 1293
Bsu36I CCTNAGG 1 cut(s) 837
BsuRI GGCC 6 cut(s) 129, 134, 138, 437, 1198, 1295
BtgI CCRYGG 1 cut(s) 33
BtgZI GCGATG 1 cut(s) 367
BtsCI GGATG 3 cut(s) 84, 464, 891
BtsIMutI CAGTG 2 cut(s) 59, 1336
BtuMI TCGCGA 1 cut(s) 386
BveI ACCTGC 2 cut(s) 589, 721
Cac8I GCNNGC 2 cut(s) 136, 774
CciI TCATGA 1 cut(s) 1161
CfoI GCGC 2 cut(s) 154, 451
Cfr10I RCCGGY 1 cut(s) 134
Cfr13I GGNCC 4 cut(s) 127, 1197, 1271, 1304
Cfr42I CCGCGG 1 cut(s) 36
CpoI CGGWCCG 1 cut(s) 1304
Csp6I GTAC 4 cut(s) 525, 923, 1027, 1124
CspI CGGWCCG 1 cut(s) 1304
CviQI GTAC 4 cut(s) 525, 923, 1027, 1124
DdeI CTNAG 4 cut(s) 681, 837, 1083, 1201
DpnI GATC 8 cut(s) 18, 183, 197, 312, 365, 404, 512, 948
DpnII GATC 8 cut(s) 16, 181, 195, 310, 363, 402, 510, 946
EaeI YGGCCR 2 cut(s) 435, 1293
EagI CGGCCG 1 cut(s) 1293
Eam1104I CTCTTC 2 cut(s) 174, 545
EarI CTCTTC 2 cut(s) 174, 545
EciI GGCGGA 3 cut(s) 39, 162, 323
EclXI CGGCCG 1 cut(s) 1293
Eco130I CCWWGG 1 cut(s) 974
Eco32I GATATC 1 cut(s) 863
Eco47I GGWCC 2 cut(s) 1271, 1304
Eco52I CGGCCG 1 cut(s) 1293
Eco81I CCTNAGG 1 cut(s) 837
Eco88I CYCGRG 2 cut(s) 269, 819
Eco91I GGTNACC 3 cut(s) 106, 551, 899
EcoO65I GGTNACC 3 cut(s) 106, 551, 899
EcoRII CCWGG 3 cut(s) 129, 895, 953
EcoRV GATATC 1 cut(s) 863
EcoT14I CCWWGG 1 cut(s) 974
ErhI CCWWGG 1 cut(s) 974
FaqI GGGAC 1 cut(s) 670
FauI CCCGC 1 cut(s) 272
FbaI TGATCA 1 cut(s) 946
FokI GGATG 3 cut(s) 71, 471, 878
FseI GGCCGGCC 1 cut(s) 138
FspBI CTAG 3 cut(s) 516, 1092, 1336
GlaI GCGC 2 cut(s) 153, 450
HaeII RGCGCY 1 cut(s) 452
HaeIII GGCC 6 cut(s) 129, 134, 138, 437, 1198, 1295
HapII CCGG 3 cut(s) 135, 1302, 1307
HhaI GCGC 2 cut(s) 154, 451
Hin6I GCGC 2 cut(s) 152, 449
HinP1I GCGC 2 cut(s) 152, 449
HincII GTYRAC 1 cut(s) 49
HindII GTYRAC 1 cut(s) 49
HindIII AAGCTT 1 cut(s) 798
HinfI GANTC 3 cut(s) 683, 692, 1175
HpaII CCGG 3 cut(s) 135, 1302, 1307
HphI GGTGA 6 cut(s) 133, 139, 193, 745, 747, 1035
Hpy166II GTNNAC 4 cut(s) 49, 349, 940, 996
Hpy188I TCNGA 5 cut(s) 181, 341, 682, 722, 1086
Hpy188III TCNNGA 5 cut(s) 319, 385, 836, 1049, 1162
Hpy8I GTNNAC 4 cut(s) 49, 349, 940, 996
Hpy99I CGWCG 8 cut(s) 215, 218, 221, 230, 236, 304, 307, 1268
HpyAV CCTTC 3 cut(s) 457, 1040, 1258
HpyCH4III ACNGT 1 cut(s) 205
HpyCH4V TGCA 5 cut(s) 70, 730, 887, 940, 1112
HpyF10VI GCNNNNNNNGC 7 cut(s) 35, 135, 228, 243, 249, 590, 1106
HpyF3I CTNAG 4 cut(s) 681, 837, 1083, 1201
HspAI GCGC 2 cut(s) 152, 449
KroI GCCGGC 1 cut(s) 134
KroNI GCCGGC 1 cut(s) 136
Ksp22I TGATCA 1 cut(s) 946
KspI CCGCGG 1 cut(s) 36
Kzo9I GATC 8 cut(s) 16, 181, 195, 310, 363, 402, 510, 946
LmnI GCTCC 5 cut(s) 26, 162, 589, 831, 1244
Lsp1109I GCAGC 5 cut(s) 82, 255, 1096, 1118, 1181
LweI GCATC 2 cut(s) 686, 1143
MaeI CTAG 3 cut(s) 516, 1092, 1336
MaeIII GTNAC 6 cut(s) 106, 199, 551, 778, 899, 1331
MalI GATC 8 cut(s) 18, 183, 197, 312, 365, 404, 512, 948
MbiI CCGCTC 1 cut(s) 157
MboI GATC 8 cut(s) 16, 181, 195, 310, 363, 402, 510, 946
MboII GAAGA 3 cut(s) 161, 532, 870
MfeI CAATTG 2 cut(s) 603, 669
MflI RGATCY 1 cut(s) 310
MhlI GDGCHC 4 cut(s) 294, 828, 942, 963
MlsI TGGCCA 1 cut(s) 437
MluCI AATT 6 cut(s) 443, 603, 669, 918, 1001, 1009
MluNI TGGCCA 1 cut(s) 437
MlyI GAGTC 1 cut(s) 692
MmeI TCCRAC 3 cut(s) 71, 688, 934
Mox20I TGGCCA 1 cut(s) 437
MroNI GCCGGC 1 cut(s) 134
MroXI GAANNNNTTC 1 cut(s) 799
MscI TGGCCA 1 cut(s) 437
MseI TTAA 3 cut(s) 285, 359, 744
MslI CAYNNNNRTG 1 cut(s) 122
Msp20I TGGCCA 1 cut(s) 437
MspA1I CMGCKG 3 cut(s) 35, 1109, 1172
MspI CCGG 3 cut(s) 135, 1302, 1307
MspR9I CCNGG 3 cut(s) 131, 897, 955
MunI CAATTG 2 cut(s) 603, 669
Mva1269I GAATGC 1 cut(s) 887
MvaI CCWGG 3 cut(s) 131, 897, 955
MvnI CGCG 4 cut(s) 35, 152, 210, 386
MwoI GCNNNNNNNGC 7 cut(s) 35, 135, 228, 243, 249, 590, 1106
NaeI GCCGGC 1 cut(s) 136
NdeII GATC 8 cut(s) 16, 181, 195, 310, 363, 402, 510, 946
NgoMIV GCCGGC 1 cut(s) 134
NlaIV GGNNCC 4 cut(s) 291, 312, 712, 844
NmeAIII GCCGAG 1 cut(s) 1271
NmuCI GTSAC 2 cut(s) 199, 1331
NruI TCGCGA 1 cut(s) 386
NspV TTCGAA 1 cut(s) 988
PaeR7I CTCGAG 1 cut(s) 819
PagI TCATGA 1 cut(s) 1161
PaqCI CACCTGC 1 cut(s) 721
PcsI WCGNNNNNNNCGW 2 cut(s) 222, 311
PctI GAATGC 1 cut(s) 887
PdiI GCCGGC 1 cut(s) 136
PdmI GAANNNNTTC 1 cut(s) 799
PfeI GAWTC 2 cut(s) 692, 1175
PleI GAGTC 1 cut(s) 691
PpsI GAGTC 1 cut(s) 691
Psp6I CCWGG 3 cut(s) 129, 895, 953
PspEI GGTNACC 3 cut(s) 106, 551, 899
PspGI CCWGG 3 cut(s) 129, 895, 953
PspN4I GGNNCC 4 cut(s) 291, 312, 712, 844
PspPI GGNCC 4 cut(s) 127, 1197, 1271, 1304
PspXI VCTCGAGB 1 cut(s) 819
PstI CTGCAG 1 cut(s) 72
PsuI RGATCY 1 cut(s) 310
PvuII CAGCTG 2 cut(s) 1109, 1172
RigI GGCCGGCC 1 cut(s) 138
RruI TCGCGA 1 cut(s) 386
RsaI GTAC 4 cut(s) 526, 924, 1028, 1125
RsaNI GTAC 4 cut(s) 525, 923, 1027, 1124
RseI CAYNNNNRTG 1 cut(s) 122
Rsr2I CGGWCCG 1 cut(s) 1304
RsrII CGGWCCG 1 cut(s) 1304
SacII CCGCGG 1 cut(s) 36
SaqAI TTAA 3 cut(s) 285, 359, 744
Sau3AI GATC 8 cut(s) 16, 181, 195, 310, 363, 402, 510, 946
Sau96I GGNCC 4 cut(s) 127, 1197, 1271, 1304
SchI GAGTC 1 cut(s) 692
ScrFI CCNGG 3 cut(s) 131, 897, 955
SduI GDGCHC 4 cut(s) 294, 828, 942, 963
SfaNI GCATC 2 cut(s) 686, 1143
SfcI CTRYAG 2 cut(s) 68, 1323
SfiI GGCCNNNNNGGCC 1 cut(s) 135
Sfr274I CTCGAG 1 cut(s) 819
Sfr303I CCGCGG 1 cut(s) 36
SfuI TTCGAA 1 cut(s) 988
SgrBI CCGCGG 1 cut(s) 36
SinI GGWCC 2 cut(s) 1271, 1304
SlaI CTCGAG 1 cut(s) 819
SmiMI CAYNNNNRTG 1 cut(s) 122
SmlI CTYRAG 1 cut(s) 819
SmoI CTYRAG 1 cut(s) 819
SpeI ACTAGT 1 cut(s) 1091
Sse9I AATT 6 cut(s) 443, 603, 669, 918, 1001, 1009
SspMI CTAG 3 cut(s) 516, 1092, 1336
StyD4I CCNGG 3 cut(s) 129, 895, 953
StyI CCWWGG 1 cut(s) 974
TaaI ACNGT 1 cut(s) 205
TaqI TCGA 5 cut(s) 299, 820, 988, 1023, 1184
TasI AATT 6 cut(s) 443, 603, 669, 918, 1001, 1009
TatI WGTACW 1 cut(s) 1123
TauI GCSGC 7 cut(s) 12, 38, 41, 157, 225, 1198, 1298
TfiI GAWTC 2 cut(s) 692, 1175
Tru1I TTAA 3 cut(s) 285, 359, 744
Tru9I TTAA 3 cut(s) 285, 359, 744
TscAI CASTG 2 cut(s) 66, 1336
TseFI GTSAC 2 cut(s) 199, 1331
TseI GCWGC 5 cut(s) 70, 243, 1106, 1109, 1169
Tsp45I GTSAC 2 cut(s) 199, 1331
TspDTI ATGAA 8 cut(s) 70, 669, 792, 861, 880, 1003, 1178, 1249
TspGWI ACGGA 5 cut(s) 77, 246, 303, 583, 870
TspRI CASTG 2 cut(s) 66, 1336
VneI GTGCAC 1 cut(s) 938
VpaK11BI GGWCC 2 cut(s) 1271, 1304
XcmI CCANNNNNNNNNTGG 1 cut(s) 121
XhoI CTCGAG 1 cut(s) 819
XmnI GAANNNNTTC 1 cut(s) 799
XspI CTAG 3 cut(s) 516, 1092, 1336
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.