Basic Information

Type: gene

Biological Identity

Species

rosa_multiflora

Chromosome

Rmu_sc0001197.1

Orthogroup ID

OG0002900

Physical Location & Seq

Genomic Coordinates Forward (+)

40516 .. 60899

Gene Structure 20384 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4275 bp

atgatggattcggacccatcacacaccaggaacgccgccaacgctggcctgcattctctatccctatctcaaaacgatataagtgttttggaaggttttgctgatgctgggtacctctctaacccacacaaagcgaaagcgaaccaaaccaaaatgacgtcgtttctggctttcctcgctgctctttccttcttacagctgctcttctttggaccttcgttgagtgcagagaagactgtctcgcctggtcttctcacttctgacgtcacttctcggaaaacacactcggaagactactgtgcaatgtatgatatatgtggagaacgcagtgacggcaaagtcttgaattgcccttatggttccccctctgtgaagctgctcttctttggaccttcgttgagtgcagagaagactgtctcgcctggtcttctcacttctgacctgctcttctttggaccttcgttgagtgcagagaagactgtctcgcctggtcttctcacttctgacgtcacttctcggaaaacacactcggaagactactgtgcaatgtatgatatatgtggagaacgcagtgacggcaaagtcttgaattgcccttatggttccccctctgtgaagcctgacgagctattttctgccaaaatccaaagtttgtgtccaactataagtggaaatgtttgttgcactgagcttcagtttgaaacgttgcgagcacaggttcaacaagcaattccatttcttgtaggctgtccagcatgcttgaggaatttcctaaatttgttttgtgagctatcttgctctccaaagcagagtctgtttatcaatgtgacttctatctccgaggttagtggcaatatgactgtggatgggatcgactattacatatctgacacttttggaaaaggactgtacaactcgtgtaaggatgttaaatttggtactatgaacacacgggccatagaatttattggtgctggtgctaaaaattttaaagaatggtttgattttcttggtgagaaagcagcccttggtttccctggttcaccttatgccattgacttcaagttaaccgttcccgagtcatctaatatggagctcatgaacatgtcagtttattcatgtgctgacacttcactaggctgctcttgtggtgattgcccttcatctcctgcgtgttctaattctgaacctcctccacacaagaaagagcaatgctccataaaaattctatctattgagatcaaatgcattgatgtttcagtagccatcatgtatattcttttagtttttgcattctttgtttggggcttgttttatcggaaaagagaaaggaggagagctggatctactaaggaaccattgttgagtgccattgatgatgatgatatcaaccctgttaacttacaaaaggatgaaagttttgccagaaaggggcatgaggtggatcttcaagttacagaagaggtcgaactatcttttattcaggaatatatgtcaagctttttcaggagatatggaatctgggtttctagaaatcccactcttgtgctctttacctcggtggggattgttcttatactctgtataggtctagttcgtttccaggtggagacacggccagagaagctgtgggtaggtcctggcagcaaggcagcggaggagaaacaattctttgacagacatcttgctcccttctacagaattgagcagctcattatagcaactgtcccagattcaaagcatggcgagtcacccagtattgtcacagatgacaatattcaattactttttgacatacaagacaaggttgacggaattcgtgctaattattctggctcagtggtagctttaactgaaatttgcttgaagcctattggccaagattgtgccactcaaagcattttgcagtattttaaaatggaccctgaaaattttgacatctatggaggagttgtacatgccgagtattgctttcagcattattcttctgcagaaacatgtttaagtgcctttcaggctccacttgatccaagcactgccttgggagggttctctggaaacaattattctgaggcttctgcattcgtagtcacctacccagttaataatgcagttgatgatgtaggaaatgagaatggaaaggcactggcttgggagaaggcttttattcagttagcaaaggatgaactgctgccaatggtgctgtctcggaatctgactctctcattttcggccgaaagttcaattgaggaagagttaaaaagagaaagtactgcagatgttataaccataatagtaagctatctggtaatgtttctgtatatatctctgacattgggagatgcacctcaaatatcttctctttacctctcatccaaggtcctgcttggtttgtcaggagtattgcttgtaatgctttctgttcttggatctgttggatttttcagtgctgtgggagttaaatctacattaatcataatggaagtcattcctttccttgttttggctgttggtgtagataacatgtgtatattggtacatgctgtgaaacggcaatcaccggagttacctttagaaagacgaataagtaatgcactggttgaagttggtccatccataacactggctagtttgtctgaggttttggcctttgcggtgggaagcttcattcctatgccagcatgccgagtcttctccatgtttgctgctcttgctgttttgctggacttctttctgcaagttactgcatttgttgcattgatatactttgactttttgagagctgaggatggcagggttgattgttttccatgcataaaagtctcgtcatcctttgtggaatccactgaaggaatctatcatggaagaactggattgttggctcgctatatgaaggaagtgcatgcacgtattcttgattcatgggtggttaagatggttatcgttgctatctttgttggttttacctgggcaagcattgcattatgttctaggattgaaacgggtttggagcagcagattgtccttccccgggactcttacctccaggggtatttcaatagtatctcagagtatctcagggttggaccaccattatactttgttgtaaaggattacaactacagctcagaatcaagacatacaaaccagttatgctccattagccaatgtgaatcgaactctcttttgaatgagatatcaagagcttctttaacaccagaatcaagctacattgctaagccagctgcctcttggcttgatgattttcttgtatggatatcccccgaggcttttggttgctgtcggaagtttttaaatgcttcttattgtccacctgatgaccagccaccttgctgttcacctgatgatggaccttgtggccttggtggagtgtgcaaagattgtacaacttgctttcgccactcagatttgattgatgatcgtccatctacagcacaatttagagagaagcttccttggttccttaatgcactgccatccgctgattgcgcaaaaggaggccatggtgcttacactaatagtgtggatctcaatggttatggaaatggcattataagagcgtccgagtttcgcacctatcacacgccacttaacaaacaaggtgattatgtaaattcaatgagagcagcacgggagttcagctcaagaatgtctgattccttaaagatggatatctttccatactcggtgttctacatcttctttgagcaatatctggatatatggagaatagctttgatcaacattgctatagctcttggtgcaatcttccttgtctgtctgcttattacttccagcttatggagttcagcaattctcatacttgtcttggcaatgattgttgtggacctaatgggagtgatggccattttggatatccaactaaatgcggtttctgtagtaaatctcataatgtcgatcggaattgctgtagagttctgtgttcatataacacatgcttacttggtgaaccatggggacagaaaccaacgagcaaagaaagctttaactaaagttggaccttctgtgttcagtggaatcacacttacaaagctagttggagtgattgtcctttgcttctcaagatcagaaatttttgtggtttattatttccaaatgtacctggcgttggttgttattggtttcttgcatgggcttgtatttttaccggtggtgttgagcatgtttggtccactgaagaggcttacaaattttgagattcaagaagtagaatcttcatcaagctcaagttga

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000323 GO:0003674 GO:0005488 GO:0005496 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005634 GO:0005635 GO:0005737 GO:0005764 GO:0005773 GO:0005783 GO:0005886 GO:0005887 GO:0006082 GO:0006464 GO:0006486 GO:0006629 GO:0006807 GO:0006810 GO:0006869 GO:0007034 GO:0007041 GO:0008150 GO:0008152 GO:0008202 GO:0008206 GO:0008289 GO:0009058 GO:0009059 GO:0009100 GO:0009101 GO:0009987 GO:0010876 GO:0012505 GO:0015485 GO:0015850 GO:0015918 GO:0016020 GO:0016021 GO:0019538 GO:0019752 GO:0030301 GO:0031224 GO:0031226 GO:0031967 GO:0031975 GO:0032787 GO:0032934 GO:0033036 GO:0034645 GO:0036094 GO:0036211 GO:0042592 GO:0042632 GO:0043170 GO:0043178 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043413 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044281 GO:0044422 GO:0044424 GO:0044425 GO:0044428 GO:0044444 GO:0044446 GO:0044459 GO:0044464 GO:0048471 GO:0048878 GO:0051179 GO:0051234 GO:0055088 GO:0055092 GO:0065007 GO:0065008 GO:0070085 GO:0071702 GO:0071704 GO:0071944 GO:0097159 GO:1901135 GO:1901137 GO:1901360 GO:1901564 GO:1901566 GO:1901576 GO:1901615

KEGG Pathways

Metabolic & Signaling

ko04142 ko04979 map04142 map04979

Pfam Domains

Protein Families

NPC1_N Patched

Protein Analysis

1424

Amino Acids

156.64

Weight (kDa)

5.19

Isoelectric Point (pI)

43.55

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
NPC1_N	PF16414	179 - 410	1.2e-65	Niemann-Pick C1 N terminus
NPC1_MLD	PF22314	555 - 746	3.4e-35	NPC1, middle luminal domain
Patched	PF02460	769 - 921	1.7e-32	Patched SSD
Sterol-sensing	PF12349	791 - 940	9.7e-47	Sterol-sensing domain of SREBP cleavage-activation

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0002900)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G38350 AT4G38350
fragaria_vesca	FvH4_2g16650 FvH4_2g16650 FvH4_2g16650 FvH4_2g16650
malus_domestica	MD10G1139800.v1.1
prunus_persica	Prupe.8G178600_v2.0.a1 Prupe.8G178600_v2.0.a1 Prupe.8G178600_v2.0.a1 Prupe.8G178600_v2.0.a1 Prupe.8G178600_v2.0.a1 Prupe.8G178600_v2.0.a1 Prupe.8G178600_v2.0.a1 Prupe.8G178600_v2.0.a1 Prupe.8G178600_v2.0.a1 Prupe.8G178600_v2.0.a1 Prupe.8G178600_v2.0.a1 Prupe.8G178600_v2.0.a1 Prupe.8G178600_v2.0.a1 Prupe.8G178600_v2.0.a1 Prupe.8G178600_v2.0.a1 Prupe.8G178600_v2.0.a1 Prupe.8G178600_v2.0.a1 Prupe.8G178600_v2.0.a1 Prupe.8G178600_v2.0.a1 Prupe.8G178600_v2.0.a1 Prupe.8G178600_v2.0.a1
rosa_chinensis	RchiOBHm_Chr6g0280781
rosa_laevigata	RLG00000013029
rosa_multiflora	Rmu_sc0001197.1_g000010
rosa_roxburghii	Rroxscaffold_7G00187670
rosa_rugosa	Rorug06G0134400
rosa_samantha	Rh6AG247100 Rh6BG249400 Rh6CG250500 Rh6DG241900
rosa_wichuraiana	Rw6G021400

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	2285, 3575
AasI	GACNNNNNNGTC	2 cut(s)	338, 581
AatII	GACGTC	3 cut(s)	161, 267, 510
Acc16I	TGCGCA	1 cut(s)	3511
Acc36I	ACCTGC	1 cut(s)	450
Acc65I	GGTACC	1 cut(s)	111
AccB1I	GGYRCC	1 cut(s)	111
AccB7I	CCANNNNNTGG	1 cut(s)	3822
AciI	CCGC	5 cut(s)	36, 1655, 2654, 3501, 3911
AclI	AACGTT	1 cut(s)	704
AclWI	GGATC	6 cut(s)	878, 1351, 1452, 2021, 2437, 3555
AcoI	YGGCCR	4 cut(s)	1616, 1876, 2232, 3885
AcuI	CTGAAG	3 cut(s)	677, 2868, 4238
AcyI	GRCGYC	3 cut(s)	158, 264, 507
AfaI	GTAC	8 cut(s)	113, 911, 940, 1956, 2272, 2538, 3406, 4142
AfiI	CCNNNNNNNGG	8 cut(s)	1431, 1563, 2046, 2503, 3031, 3368, 3822, 4150
AflIII	ACRYGT	3 cut(s)	1104, 1997, 2523
AgeI	ACCGGT	1 cut(s)	4189
AleI	CACNNNNGTG	1 cut(s)	1544
Alw21I	GWGCWC	3 cut(s)	715, 1098, 1551
Alw26I	GTCTC	6 cut(s)	244, 421, 487, 1604, 2211, 2827
AlwI	GGATC	6 cut(s)	878, 1351, 1452, 2021, 2437, 3555
AlwNI	CAGNNNCTG	1 cut(s)	814
Ama87I	CYCGRG	3 cut(s)	1076, 3030, 3284
AoxI	GGCC	9 cut(s)	46, 954, 1616, 1876, 2232, 2646, 3379, 3520, 3885
ArsI	GACNNNNNNTTYG	2 cut(s)	640, 672
AseI	ATTAAT	1 cut(s)	2471
AsiGI	ACCGGT	1 cut(s)	4189
Asp700I	GAANNNNTTC	2 cut(s)	1667, 2487
Asp718I	GGTACC	1 cut(s)	111
AspLEI	GCGC	1 cut(s)	3512
AsuC2I	CCSGG	2 cut(s)	3031, 3032
AsuHPI	GGTGA	9 cut(s)	1025, 1035, 1163, 1743, 2083, 2550, 3351, 3635, 4000
AvaI	CYCGRG	3 cut(s)	1076, 3030, 3284
BalI	TGGCCA	2 cut(s)	1878, 3887
BanI	GGYRCC	1 cut(s)	111
BanII	GRGCYC	1 cut(s)	1098
BauI	CACGAG	1 cut(s)	916
BbsI	GAAGAC	9 cut(s)	239, 242, 297, 416, 419, 482, 485, 540, 2683
Bbv12I	GWGCWC	3 cut(s)	715, 1098, 1551
BbvCI	CCTCAGC	1 cut(s)	2784
BceAI	ACGGC	4 cut(s)	349, 592, 1631, 2567
BclI	TGATCA	1 cut(s)	3759
BcnI	CCSGG	2 cut(s)	3031, 3032
BcoDI	GTCTC	6 cut(s)	244, 421, 487, 1604, 2211, 2827
BfaI	CTAG	6 cut(s)	1136, 1530, 1592, 2628, 2991, 4076
BfmI	CTRYAG	8 cut(s)	1696, 1989, 2274, 3121, 3450, 3771, 3918, 3951
BfuAI	ACCTGC	1 cut(s)	450
BglI	GCCNNNNNGGC	1 cut(s)	2015
BlpI	GCTNAGC	1 cut(s)	3237
BmcAI	AGTACT	1 cut(s)	2272
BmeT110I	CYCGRG	3 cut(s)	1076, 3030, 3284
BmiI	GGNNCC	8 cut(s)	15, 113, 361, 604, 1356, 1923, 2019, 3482
BmrI	ACTGGG	2 cut(s)	1749, 2093
BmsI	GCATC	2 cut(s)	94, 2332
BmuI	ACTGGG	2 cut(s)	1749, 2093
BpiI	GAAGAC	9 cut(s)	239, 242, 297, 416, 419, 482, 485, 540, 2683
BplI	GAGNNNNNCTC	4 cut(s)	1199, 1231, 3647, 3679
BpmI	CTGGAG	1 cut(s)	3029
Bpu10I	CCTNAGC	1 cut(s)	2784
Bpu1102I	GCTNAGC	1 cut(s)	3237
BpuEI	CTTGAG	4 cut(s)	781, 3649, 4087, 4252
BpuMI	CCSGG	2 cut(s)	3031, 3032
BsaAI	YACGTR	1 cut(s)	2909
BsaBI	GATNNNNATC	2 cut(s)	2939, 3692
BsaHI	GRCGYC	3 cut(s)	158, 264, 507
BsaWI	WCCGGW	2 cut(s)	2560, 4189
BsaXI	ACNNNNNCTCC	4 cut(s)	1938, 1968, 4074, 4104
Bsc4I	CCNNNNNNNGG	8 cut(s)	1431, 1563, 2046, 2503, 3031, 3368, 3822, 4150
Bse118I	RCCGGY	1 cut(s)	4189
Bse1I	ACTGG	7 cut(s)	1755, 2099, 2151, 2601, 2628, 2875, 3148
Bse3DI	GCAATG	7 cut(s)	309, 552, 1217, 2976, 3231, 3765, 3861
Bse8I	GATNNNNATC	2 cut(s)	2939, 3692
BseGI	GGATG	9 cut(s)	871, 931, 1417, 2188, 2372, 2612, 2794, 2828, 3497
BseJI	GATNNNNATC	2 cut(s)	2939, 3692
BseLI	CCNNNNNNNGG	8 cut(s)	1431, 1563, 2046, 2503, 3031, 3368, 3822, 4150
BseMI	GCAATG	7 cut(s)	309, 552, 1217, 2976, 3231, 3765, 3861
BseMII	CTCAG	9 cut(s)	678, 1851, 2061, 2628, 2775, 3081, 3091, 3141, 3438
BseNI	ACTGG	7 cut(s)	1755, 2099, 2151, 2601, 2628, 2875, 3148
BseRI	GAGGAG	4 cut(s)	1182, 1348, 1673, 1962
BseX3I	CGGCCG	1 cut(s)	2232
BseYI	CCCAGC	1 cut(s)	107
BsgI	GTGCAG	3 cut(s)	246, 423, 489
Bsh1285I	CGRYCG	2 cut(s)	2235, 3942
BshFI	GGCC	9 cut(s)	48, 956, 1618, 1878, 2234, 2648, 3381, 3522, 3887
BshNI	GGYRCC	1 cut(s)	111
BshTI	ACCGGT	1 cut(s)	4189
BsiEI	CGRYCG	2 cut(s)	2235, 3942
BsiHKAI	GWGCWC	3 cut(s)	715, 1098, 1551
BsiHKCI	CYCGRG	3 cut(s)	1076, 3030, 3284
BsiSI	CCGG	3 cut(s)	2561, 3031, 4190
BslFI	GGGAC	3 cut(s)	1712, 3047, 4013
BslI	CCNNNNNNNGG	8 cut(s)	1431, 1563, 2046, 2503, 3031, 3368, 3822, 4150
BsmAI	GTCTC	6 cut(s)	244, 421, 487, 1604, 2211, 2827
BsmFI	GGGAC	3 cut(s)	1712, 3047, 4013
BsmI	GAATGC	3 cut(s)	52, 1292, 2081
BsnI	GGCC	9 cut(s)	48, 956, 1618, 1878, 2234, 2648, 3381, 3522, 3887
BsoBI	CYCGRG	3 cut(s)	1076, 3030, 3284
Bsp1286I	GDGCHC	3 cut(s)	715, 1098, 1551
Bsp1407I	TGTACA	3 cut(s)	909, 1954, 3404
Bsp1720I	GCTNAGC	1 cut(s)	3237
Bsp19I	CCATGG	2 cut(s)	3523, 3994
BspACI	CCGC	5 cut(s)	36, 1655, 2654, 3501, 3911
BspANI	GGCC	9 cut(s)	48, 956, 1618, 1878, 2234, 2648, 3381, 3522, 3887
BspCNI	CTCAG	9 cut(s)	679, 1850, 2062, 2629, 2776, 3080, 3090, 3140, 3437
BspHI	TCATGA	1 cut(s)	1098
BspLI	GGNNCC	8 cut(s)	15, 113, 361, 604, 1356, 1923, 2019, 3482
BspMAI	CTGCAG	2 cut(s)	1993, 2278
BspMI	ACCTGC	1 cut(s)	450
BspPI	GGATC	6 cut(s)	878, 1351, 1452, 2021, 2437, 3555
BspQI	GCTCTTC	3 cut(s)	209, 386, 452
BspT107I	GGYRCC	1 cut(s)	111
BsrDI	GCAATG	7 cut(s)	309, 552, 1217, 2976, 3231, 3765, 3861
BsrFI	RCCGGY	1 cut(s)	4189
BsrGI	TGTACA	3 cut(s)	909, 1954, 3404
BsrI	ACTGG	7 cut(s)	1755, 2099, 2151, 2601, 2628, 2875, 3148
BssAI	RCCGGY	1 cut(s)	4189
BssNI	GRCGYC	3 cut(s)	158, 264, 507
BssSI	CACGAG	1 cut(s)	916
BssT1I	CCWWGG	7 cut(s)	1027, 2040, 2376, 3382, 3476, 3523, 3994
Bst2BI	CACGAG	1 cut(s)	916
Bst4CI	ACNGT	9 cut(s)	238, 299, 415, 481, 542, 862, 909, 1072, 1726
Bst6I	CTCTTC	6 cut(s)	209, 386, 452, 1455, 2247, 4214
BstACI	GRCGYC	3 cut(s)	158, 264, 507
BstAPI	GCANNNNNTGC	2 cut(s)	2753, 2978
BstAUI	TGTACA	3 cut(s)	909, 1954, 3404
BstBAI	YACGTR	1 cut(s)	2909
BstDSI	CCRYGG	2 cut(s)	3523, 3994
BstF5I	GGATG	9 cut(s)	871, 931, 1417, 2188, 2372, 2612, 2794, 2828, 3497
BstHHI	GCGC	1 cut(s)	3512
BstMAI	GTCTC	6 cut(s)	244, 421, 487, 1604, 2211, 2827
BstMCI	CGRYCG	2 cut(s)	2235, 3942
BstSFI	CTRYAG	8 cut(s)	1696, 1989, 2274, 3121, 3450, 3771, 3918, 3951
BstV2I	GAAGAC	9 cut(s)	239, 242, 297, 416, 419, 482, 485, 540, 2683
BstX2I	RGATCY	4 cut(s)	1343, 1444, 2429, 3547
BstXI	CCANNNNNNTGG	1 cut(s)	2623
BstYI	RGATCY	4 cut(s)	1343, 1444, 2429, 3547
BstZI	CGGCCG	1 cut(s)	2232
BsuRI	GGCC	9 cut(s)	48, 956, 1618, 1878, 2234, 2648, 3381, 3522, 3887
BtgI	CCRYGG	2 cut(s)	3523, 3994
BtsCI	GGATG	9 cut(s)	871, 931, 1417, 2188, 2372, 2612, 2794, 2828, 3497
BtsI	GCAGTG	4 cut(s)	334, 577, 2034, 3491
BveI	ACCTGC	1 cut(s)	450
CaiI	CAGNNNCTG	1 cut(s)	814
CciI	TCATGA	1 cut(s)	1098
CfoI	GCGC	1 cut(s)	3512
Cfr10I	RCCGGY	1 cut(s)	4189
Cfr9I	CCCGGG	1 cut(s)	3030
CseI	GACGC	1 cut(s)	3570
Csp6I	GTAC	8 cut(s)	112, 910, 939, 1955, 2271, 2537, 3405, 4141
CspAI	ACCGGT	1 cut(s)	4189
CviQI	GTAC	8 cut(s)	112, 910, 939, 1955, 2271, 2537, 3405, 4141
DraI	TTTAAA	3 cut(s)	991, 1915, 3315
DrdI	GACNNNNNNGTC	2 cut(s)	338, 581
DseDI	GACNNNNNNGTC	2 cut(s)	338, 581
EaeI	YGGCCR	4 cut(s)	1616, 1876, 2232, 3885
EagI	CGGCCG	1 cut(s)	2232
Eam1104I	CTCTTC	6 cut(s)	209, 386, 452, 1455, 2247, 4214
EarI	CTCTTC	6 cut(s)	209, 386, 452, 1455, 2247, 4214
Ecl136II	GAGCTC	1 cut(s)	1096
EclXI	CGGCCG	1 cut(s)	2232
Eco130I	CCWWGG	7 cut(s)	1027, 2040, 2376, 3382, 3476, 3523, 3994
Eco24I	GRGCYC	1 cut(s)	1098
Eco32I	GATATC	5 cut(s)	1387, 3198, 3279, 3694, 3898
Eco52I	CGGCCG	1 cut(s)	2232
Eco53kI	GAGCTC	1 cut(s)	1096
Eco57I	CTGAAG	3 cut(s)	677, 2868, 4238
Eco88I	CYCGRG	3 cut(s)	1076, 3030, 3284
EcoICRI	GAGCTC	1 cut(s)	1096
EcoO109I	RGGNCCY	2 cut(s)	1637, 2380
EcoRI	GAATTC	1 cut(s)	1815
EcoRV	GATATC	5 cut(s)	1387, 3198, 3279, 3694, 3898
EcoT14I	CCWWGG	7 cut(s)	1027, 2040, 2376, 3382, 3476, 3523, 3994
EcoT22I	ATGCAT	2 cut(s)	1250, 2816
EcoT38I	GRGCYC	1 cut(s)	1098
ErhI	CCWWGG	7 cut(s)	1027, 2040, 2376, 3382, 3476, 3523, 3994
FalI	AAGNNNNNCTT	6 cut(s)	365, 397, 1011, 1043, 3193, 3225
FaqI	GGGAC	3 cut(s)	1712, 3047, 4013
FbaI	TGATCA	1 cut(s)	3759
FokI	GGATG	9 cut(s)	878, 938, 1424, 2195, 2359, 2599, 2801, 2815, 3484
FriOI	GRGCYC	1 cut(s)	1098
FspBI	CTAG	6 cut(s)	1136, 1530, 1592, 2628, 2991, 4076
FspI	TGCGCA	1 cut(s)	3511
GlaI	GCGC	1 cut(s)	3511
GsaI	CCCAGC	1 cut(s)	111
GsuI	CTGGAG	1 cut(s)	3029
HaeIII	GGCC	9 cut(s)	48, 956, 1618, 1878, 2234, 2648, 3381, 3522, 3887
HapII	CCGG	3 cut(s)	2561, 3031, 4190
HgaI	GACGC	1 cut(s)	3570
HhaI	GCGC	1 cut(s)	3512
Hin1I	GRCGYC	3 cut(s)	158, 264, 507
Hin6I	GCGC	1 cut(s)	3510
HinP1I	GCGC	1 cut(s)	3510
HincII	GTYRAC	3 cut(s)	1068, 1399, 1810
HindII	GTYRAC	3 cut(s)	1068, 1399, 1810
HindIII	AAGCTT	4 cut(s)	1498, 2662, 3470, 4023
HpaI	GTTAAC	2 cut(s)	1068, 1399
HpaII	CCGG	3 cut(s)	2561, 3031, 4190
HphI	GGTGA	9 cut(s)	1025, 1035, 1163, 1743, 2083, 2550, 3351, 3635, 4000
Hpy166II	GTNNAC	9 cut(s)	1043, 1068, 1399, 1810, 3332, 3359, 3868, 3991, 4214
Hpy8I	GTNNAC	9 cut(s)	1043, 1068, 1399, 1810, 3332, 3359, 3868, 3991, 4214
Hpy99I	CGWCG	1 cut(s)	163
HpyCH4III	ACNGT	9 cut(s)	238, 299, 415, 481, 542, 862, 909, 1072, 1726
HpyCH4IV	ACGT	5 cut(s)	158, 264, 507, 704, 2908
HpySE526I	ACGT	5 cut(s)	158, 264, 507, 704, 2908
Hsp92I	GRCGYC	3 cut(s)	158, 264, 507
HspAI	GCGC	1 cut(s)	3510
KpnI	GGTACC	1 cut(s)	115
Ksp22I	TGATCA	1 cut(s)	3759
KspAI	GTTAAC	2 cut(s)	1068, 1399
LguI	GCTCTTC	3 cut(s)	209, 386, 452
LmnI	GCTCC	6 cut(s)	1093, 1220, 1693, 2023, 3010, 3161
LweI	GCATC	2 cut(s)	94, 2332
MaeI	CTAG	6 cut(s)	1136, 1530, 1592, 2628, 2991, 4076
MaeII	ACGT	5 cut(s)	158, 264, 507, 704, 2908
MfeI	CAATTG	1 cut(s)	2244
MflI	RGATCY	4 cut(s)	1343, 1444, 2429, 3547
MhlI	GDGCHC	3 cut(s)	715, 1098, 1551
MlsI	TGGCCA	2 cut(s)	1878, 3887
MluNI	TGGCCA	2 cut(s)	1878, 3887
MlyI	GAGTC	6 cut(s)	820, 1088, 1757, 2212, 2697, 3029
MmeI	TCCRAC	7 cut(s)	683, 2416, 3064, 3284, 3925, 4018, 4060
Mox20I	TGGCCA	2 cut(s)	1878, 3887
Mph1103I	ATGCAT	2 cut(s)	1250, 2816
MroXI	GAANNNNTTC	2 cut(s)	1667, 2487
MscI	TGGCCA	2 cut(s)	1878, 3887
MslI	CAYNNNNRTG	3 cut(s)	1103, 1544, 2672
Msp20I	TGGCCA	2 cut(s)	1878, 3887
MspA1I	CMGCKG	4 cut(s)	199, 1655, 3245, 3503
MspI	CCGG	3 cut(s)	2561, 3031, 4190
MunI	CAATTG	1 cut(s)	2244
Mva1269I	GAATGC	3 cut(s)	52, 1292, 2081
NciI	CCSGG	2 cut(s)	3031, 3032
NcoI	CCATGG	2 cut(s)	3523, 3994
NlaIV	GGNNCC	8 cut(s)	15, 113, 361, 604, 1356, 1923, 2019, 3482
NmeAIII	GCCGAG	2 cut(s)	1987, 2711
NmuCI	GTSAC	8 cut(s)	265, 329, 508, 572, 826, 1749, 1762, 2089
NsbI	TGCGCA	1 cut(s)	3511
NsiI	ATGCAT	2 cut(s)	1250, 2816
OliI	CACNNNNGTG	1 cut(s)	1544
PaeI	GCATGC	3 cut(s)	759, 2685, 2906
PagI	TCATGA	1 cut(s)	1098
PciI	ACATGT	3 cut(s)	1104, 1997, 2523
PciSI	GCTCTTC	3 cut(s)	209, 386, 452
PctI	GAATGC	3 cut(s)	52, 1292, 2081
PdmI	GAANNNNTTC	2 cut(s)	1667, 2487
PflMI	CCANNNNNTGG	1 cut(s)	3822
PinAI	ACCGGT	1 cut(s)	4189
Ple19I	CGATCG	1 cut(s)	3942
PleI	GAGTC	6 cut(s)	819, 1087, 1756, 2212, 2696, 3029
PpsI	GAGTC	6 cut(s)	819, 1087, 1756, 2212, 2696, 3029
Ppu21I	YACGTR	1 cut(s)	2909
PpuMI	RGGWCCY	2 cut(s)	1637, 2380
PscI	ACATGT	3 cut(s)	1104, 1997, 2523
PshBI	ATTAAT	1 cut(s)	2471
PsiI	TTATAA	2 cut(s)	2285, 3575
Psp124BI	GAGCTC	1 cut(s)	1098
Psp1406I	AACGTT	1 cut(s)	704
Psp5II	RGGWCCY	2 cut(s)	1637, 2380
PspFI	CCCAGC	1 cut(s)	107
PspN4I	GGNNCC	8 cut(s)	15, 113, 361, 604, 1356, 1923, 2019, 3482
PspPPI	RGGWCCY	2 cut(s)	1637, 2380
PstI	CTGCAG	2 cut(s)	1993, 2278
PstNI	CAGNNNCTG	1 cut(s)	814
PsuI	RGATCY	4 cut(s)	1343, 1444, 2429, 3547
PvuI	CGATCG	1 cut(s)	3942
PvuII	CAGCTG	2 cut(s)	199, 3245
RsaI	GTAC	8 cut(s)	113, 911, 940, 1956, 2272, 2538, 3406, 4142
RsaNI	GTAC	8 cut(s)	112, 910, 939, 1955, 2271, 2537, 3405, 4141
RseI	CAYNNNNRTG	3 cut(s)	1103, 1544, 2672
SacI	GAGCTC	1 cut(s)	1098
SapI	GCTCTTC	3 cut(s)	209, 386, 452
ScaI	AGTACT	1 cut(s)	2272
SchI	GAGTC	6 cut(s)	820, 1088, 1757, 2212, 2697, 3029
SduI	GDGCHC	3 cut(s)	715, 1098, 1551
SfaNI	GCATC	2 cut(s)	94, 2332
SfcI	CTRYAG	8 cut(s)	1696, 1989, 2274, 3121, 3450, 3771, 3918, 3951
SmaI	CCCGGG	1 cut(s)	3032
SmiMI	CAYNNNNRTG	3 cut(s)	1103, 1544, 2672
SmlI	CTYRAG	4 cut(s)	760, 3664, 4102, 4267
SmoI	CTYRAG	4 cut(s)	760, 3664, 4102, 4267
SphI	GCATGC	3 cut(s)	759, 2685, 2906
SsiI	CCGC	5 cut(s)	36, 1655, 2654, 3501, 3911
SspI	AATATT	1 cut(s)	1777
SspMI	CTAG	6 cut(s)	1136, 1530, 1592, 2628, 2991, 4076
SstI	GAGCTC	1 cut(s)	1098
StyI	CCWWGG	7 cut(s)	1027, 2040, 2376, 3382, 3476, 3523, 3994
TaaI	ACNGT	9 cut(s)	238, 299, 415, 481, 542, 862, 909, 1072, 1726
TaiI	ACGT	5 cut(s)	161, 267, 510, 707, 2911
TaqI	TCGA	4 cut(s)	873, 1467, 3176, 3938
TatI	WGTACW	4 cut(s)	909, 1954, 2270, 3404
TauI	GCSGC	1 cut(s)	38
TseFI	GTSAC	8 cut(s)	265, 329, 508, 572, 826, 1749, 1762, 2089
Tsp45I	GTSAC	8 cut(s)	265, 329, 508, 572, 826, 1749, 1762, 2089
TspGWI	ACGGA	1 cut(s)	1827
TspMI	CCCGGG	1 cut(s)	3030
Van91I	CCANNNNNTGG	1 cut(s)	3822
VspI	ATTAAT	1 cut(s)	2471
XbaI	TCTAGA	1 cut(s)	1529
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1626, 3249
XmaI	CCCGGG	1 cut(s)	3030
XmnI	GAANNNNTTC	2 cut(s)	1667, 2487
XspI	CTAG	6 cut(s)	1136, 1530, 1592, 2628, 2991, 4076
ZraI	GACGTC	3 cut(s)	159, 265, 508
ZrmI	AGTACT	1 cut(s)	2272
Zsp2I	ATGCAT	2 cut(s)	1250, 2816

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.