Rmu_sc0001200.1_g000040

Ammonium transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001200.1
OG0016977
Physical Location & Seq
Forward (+)
215026 .. 223544
8519 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 816 bp
atgtttgcggccataacaccgatactgttggcagggtcggtgctagggagaatgaacttcaaggcgtggatgatgttcatgcctctgtggctgaccttctcgtacaccgtcggtgcgtttagcatgtggggcggagggtttttatttcactggggagttatggactacgctggcgggtacttcattcatctttcttcagggactgctggtttcatcactgcttactgggtggccggacaacggcaaaatgaaggggagaaggagcgtgatggcggcaaaacgaaggggagaaggagagaggccaatgcgggggaagagagagaaactccggggggggagggtgagaaagagaaggtagagcctgaaattgttgttgtgaatgaagaaaagaaaacaggagaatatgagaagaagggtaaggtacttgcatctgaggattctcggcaacattggaatggtttcaagcctgggcatttccaacgcaatacttctgacatagtatccgcctttcagaagacctgcattttggaagaagacctgcatctgtctggattggtgtccttgatggacctggaattggtgaaaaagggttttgatgattatgcccggaaaaggaagaagtggctgtttgtaatggctgctttgggcttctctggttatggtgcttatagggtttatcactcgccttcagtatgtcaaaaaagaaagaggcttttgaaccttttaggtgctttagtttctgtagttgaagtggtttcagactctgctgagtcgattggaattgtgtctaaagatttgaaggagtctgcaatttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ammonium_transp

Protein Analysis

271

Amino Acids

30.24

Weight (kDa)

8.67

Isoelectric Point (pI)

31.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ammonium_transp PF00909 1 - 88 1.9e-17 Ammonium Transporter Family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 527, 546
AciI CCGC 6 cut(s) 8, 132, 174, 273, 308, 504
AcoI YGGCCR 2 cut(s) 9, 231
AcuI CTGAAG 2 cut(s) 180, 672
AfaI GTAC 3 cut(s) 104, 179, 423
AfiI CCNNNNNNNGG 4 cut(s) 240, 309, 577, 612
AgsI TTSAA 5 cut(s) 61, 463, 718, 749, 799
AjnI CCWGG 2 cut(s) 466, 570
AlwNI CAGNNNCTG 2 cut(s) 203, 764
AoxI GGCC 3 cut(s) 9, 231, 300
ApeKI GCWGC 1 cut(s) 638
ArsI GACNNNNNNTTYG 2 cut(s) 508, 540
Asp700I GAANNNNTTC 1 cut(s) 458
AspS9I GGNCC 1 cut(s) 568
AsuC2I CCSGG 2 cut(s) 330, 607
AsuHPI GGTGA 2 cut(s) 353, 592
AvaII GGWCC 1 cut(s) 568
BbsI GAAGAC 2 cut(s) 521, 540
BbvI GCAGC 1 cut(s) 625
BccI CCATC 2 cut(s) 263, 559
BceAI ACGGC 1 cut(s) 257
BciT130I CCWGG 2 cut(s) 468, 572
BciVI GTATCC 1 cut(s) 511
BcnI CCSGG 2 cut(s) 330, 607
BfaI CTAG 1 cut(s) 44
BfmI CTRYAG 1 cut(s) 741
BfuAI ACCTGC 2 cut(s) 527, 546
BfuI GTATCC 1 cut(s) 511
BglI GCCNNNNNGGC 1 cut(s) 88
BisI GCNGC 3 cut(s) 9, 274, 639
BlsI GCNGC 3 cut(s) 10, 275, 640
Bme1390I CCNGG 4 cut(s) 330, 468, 572, 607
Bme18I GGWCC 1 cut(s) 568
BmgT120I GGNCC 1 cut(s) 568
BmrFI CCNGG 4 cut(s) 330, 468, 572, 607
BmrI ACTGGG 2 cut(s) 160, 235
BmsI GCATC 2 cut(s) 437, 550
BmuI ACTGGG 2 cut(s) 160, 235
BpiI GAAGAC 2 cut(s) 521, 540
BplI GAGNNNNNCTC 2 cut(s) 310, 342
BpuMI CCSGG 2 cut(s) 330, 607
BsaJI CCNNGG 2 cut(s) 329, 467
Bsc4I CCNNNNNNNGG 4 cut(s) 240, 309, 577, 612
Bse1I ACTGG 2 cut(s) 155, 230
BseBI CCWGG 2 cut(s) 468, 572
BseDI CCNNGG 2 cut(s) 329, 467
BseGI GGATG 1 cut(s) 75
BseLI CCNNNNNNNGG 4 cut(s) 240, 309, 577, 612
BseMII CTCAG 2 cut(s) 423, 759
BseNI ACTGG 2 cut(s) 155, 230
BseXI GCAGC 1 cut(s) 625
BshFI GGCC 3 cut(s) 11, 233, 302
BsiSI CCGG 3 cut(s) 234, 329, 607
BslFI GGGAC 1 cut(s) 214
BslI CCNNNNNNNGG 4 cut(s) 240, 309, 577, 612
BsmFI GGGAC 1 cut(s) 214
BsnI GGCC 3 cut(s) 11, 233, 302
BspACI CCGC 6 cut(s) 8, 132, 174, 273, 308, 504
BspANI GGCC 3 cut(s) 11, 233, 302
BspCNI CTCAG 2 cut(s) 424, 760
BspMI ACCTGC 2 cut(s) 527, 546
BsrI ACTGG 2 cut(s) 155, 230
BssECI CCNNGG 2 cut(s) 329, 467
Bst2UI CCWGG 2 cut(s) 468, 572
Bst4CI ACNGT 2 cut(s) 27, 109
Bst6I CTCTTC 1 cut(s) 309
BstC8I GCNNGC 1 cut(s) 172
BstDEI CTNAG 2 cut(s) 432, 768
BstF5I GGATG 1 cut(s) 75
BstMWI GCNNNNNNNGC 2 cut(s) 88, 129
BstNI CCWGG 2 cut(s) 468, 572
BstNSI RCATGY 1 cut(s) 127
BstSCI CCNGG 4 cut(s) 328, 466, 570, 605
BstSFI CTRYAG 1 cut(s) 741
BstV1I GCAGC 1 cut(s) 625
BstV2I GAAGAC 2 cut(s) 521, 540
BsuI GTATCC 1 cut(s) 511
BsuRI GGCC 3 cut(s) 11, 233, 302
BtsCI GGATG 1 cut(s) 75
BtsI GCAGTG 1 cut(s) 216
BtsIMutI CAGTG 2 cut(s) 148, 216
BveI ACCTGC 2 cut(s) 527, 546
Cac8I GCNNGC 1 cut(s) 172
CaiI CAGNNNCTG 2 cut(s) 203, 764
Cfr13I GGNCC 1 cut(s) 568
Csp6I GTAC 3 cut(s) 103, 178, 422
CviAII CATG 2 cut(s) 79, 124
CviQI GTAC 3 cut(s) 103, 178, 422
DdeI CTNAG 2 cut(s) 432, 768
EaeI YGGCCR 2 cut(s) 9, 231
Eam1104I CTCTTC 1 cut(s) 309
EarI CTCTTC 1 cut(s) 309
EciI GGCGGA 2 cut(s) 147, 493
Eco47I GGWCC 1 cut(s) 568
Eco57I CTGAAG 2 cut(s) 180, 672
EcoRII CCWGG 2 cut(s) 466, 570
FaeI CATG 2 cut(s) 82, 127
FaqI GGGAC 1 cut(s) 214
FatI CATG 2 cut(s) 78, 123
FauI CCCGC 2 cut(s) 167, 301
Fnu4HI GCNGC 3 cut(s) 9, 274, 639
FokI GGATG 1 cut(s) 82
Fsp4HI GCNGC 3 cut(s) 9, 274, 639
FspBI CTAG 1 cut(s) 44
GluI GCNGC 3 cut(s) 9, 274, 639
HaeIII GGCC 3 cut(s) 11, 233, 302
HapII CCGG 3 cut(s) 234, 329, 607
Hin1II CATG 2 cut(s) 82, 127
HinfI GANTC 4 cut(s) 437, 761, 770, 803
HpaII CCGG 3 cut(s) 234, 329, 607
HphI GGTGA 2 cut(s) 353, 592
Hpy166II GTNNAC 1 cut(s) 105
Hpy188I TCNGA 4 cut(s) 433, 493, 513, 760
Hpy188III TCNNGA 1 cut(s) 549
Hpy8I GTNNAC 1 cut(s) 105
Hpy99I CGWCG 1 cut(s) 113
HpyAV CCTTC 9 cut(s) 106, 245, 253, 277, 285, 346, 406, 696, 793
HpyCH4III ACNGT 2 cut(s) 27, 109
HpyCH4V TGCA 4 cut(s) 428, 522, 541, 809
HpyF10VI GCNNNNNNNGC 2 cut(s) 88, 129
HpyF3I CTNAG 2 cut(s) 432, 768
Hsp92II CATG 2 cut(s) 82, 127
LmnI GCTCC 1 cut(s) 262
Lsp1109I GCAGC 1 cut(s) 625
LweI GCATC 2 cut(s) 437, 550
MaeI CTAG 1 cut(s) 44
MboII GAAGA 8 cut(s) 186, 326, 395, 421, 526, 542, 545, 628
MluCI AATT 4 cut(s) 366, 575, 780, 810
MlyI GAGTC 3 cut(s) 755, 779, 812
MmeI TCCRAC 1 cut(s) 502
MnlI CCTC 6 cut(s) 93, 128, 292, 331, 427, 702
MroXI GAANNNNTTC 1 cut(s) 458
MslI CAYNNNNRTG 1 cut(s) 453
MspI CCGG 3 cut(s) 234, 329, 607
MspR9I CCNGG 4 cut(s) 330, 468, 572, 607
MvaI CCWGG 2 cut(s) 468, 572
MwoI GCNNNNNNNGC 2 cut(s) 88, 129
NciI CCSGG 2 cut(s) 330, 607
NlaIII CATG 2 cut(s) 82, 127
NmeAIII GCCGAG 1 cut(s) 421
NspI RCATGY 1 cut(s) 127
PdmI GAANNNNTTC 1 cut(s) 458
PfeI GAWTC 1 cut(s) 437
PkrI GCNGC 3 cut(s) 10, 275, 640
PleI GAGTC 3 cut(s) 755, 778, 811
PpsI GAGTC 3 cut(s) 755, 778, 811
Psp6I CCWGG 2 cut(s) 466, 570
PspGI CCWGG 2 cut(s) 466, 570
PspPI GGNCC 1 cut(s) 568
PstNI CAGNNNCTG 2 cut(s) 203, 764
RsaI GTAC 3 cut(s) 104, 179, 423
RsaNI GTAC 3 cut(s) 103, 178, 422
RseI CAYNNNNRTG 1 cut(s) 453
SatI GCNGC 3 cut(s) 9, 274, 639
Sau96I GGNCC 1 cut(s) 568
SchI GAGTC 3 cut(s) 755, 779, 812
ScrFI CCNGG 4 cut(s) 330, 468, 572, 607
SetI ASST 8 cut(s) 98, 357, 423, 521, 540, 573, 723, 730
SfaNI GCATC 2 cut(s) 437, 550
SfcI CTRYAG 1 cut(s) 741
SinI GGWCC 1 cut(s) 568
SmiMI CAYNNNNRTG 1 cut(s) 453
Sse9I AATT 4 cut(s) 366, 575, 780, 810
SsiI CCGC 6 cut(s) 8, 132, 174, 273, 308, 504
SspMI CTAG 1 cut(s) 44
StyD4I CCNGG 4 cut(s) 328, 466, 570, 605
TaaI ACNGT 2 cut(s) 27, 109
TaqI TCGA 1 cut(s) 773
TasI AATT 4 cut(s) 366, 575, 780, 810
TauI GCSGC 2 cut(s) 11, 276
TfiI GAWTC 1 cut(s) 437
TscAI CASTG 2 cut(s) 155, 223
TseI GCWGC 1 cut(s) 638
TspDTI ATGAA 7 cut(s) 67, 68, 172, 176, 202, 264, 396
TspRI CASTG 2 cut(s) 155, 223
VpaK11BI GGWCC 1 cut(s) 568
XceI RCATGY 1 cut(s) 127
XmnI GAANNNNTTC 1 cut(s) 458
XspI CTAG 1 cut(s) 44
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.