Rmu_sc0001211.1_g000048

Anoctamin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001211.1
OG0006221
Physical Location & Seq
Reverse (-)
170461 .. 175722
5262 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2079 bp
atgaatgttgttgaggaggaacaaattccttttgagattggggttgtggttccaaagagaagtgtcaaggcaaaagaagaagatgactgtgtggaggttttggttgatgaatttaagaatgtgggtttggtagttgagagagtccctggcgttgtcgatgactttatcaagctggcagcgcctttagagatattggggaaggctgcggttgagttgcaaattaaaaagcgaactcaaattggtaatgatctagctgctccattgaagttgctggcagcgcctttagagatattggggaaggctgcgtttgagttgcaaattaaaaagcgaactcaaattgggatggatttacaatttgaatgggaggaggttgatgcttttgtgaggcagcctgatggttcattgttcagctggtgtgagcgttttcattgctataggcacttgatatatggaattatgaacaagagcaagtcagctagaactctgagatttgatgggaaagagttctattgggaagttcgggaatgtttgcttaagaaattggagtcacagaggattgtcaaagaagtttttcctttgcatgatgaagtgaaaaggaagaaactccttcactcttgggctctgcactggtgggacttcacaagccagcccatagatgagatttactcatattttggtacaaagattgccatctattttgcttttcttggaatgtatacacggtggttgctgtttccagctacatttggacttattctgcagatatttgattttgggtcgttgcagttactggtgctcccccttttctttataagcataaccttatgggctataatgttttcccagttctggaaacgcaaaaatgttgctctgttaactagatggcagatcagttatccaattagaaccgacctgggaaataggcagctgggcatggcttggagttcctcgcagtcgtctgtagaactcatgaagaaatgggggacagacaaaacaaaagagaaagaaatgtttcaaagaattgagtggtttggacgactactgagattccgaaatgatgctatcattatagttagtattatatgccttcagttgccgtttgagctggcatatgctcatctgtacgaggttattaagtctgatattgtgaagtttgggttgacaaccatatatctttttgcaattcagttttttacgaagctagggggcaagatctctgttaagcttattaaacacgaaaataatgaaaacacagaaaaaagagctgacagcttggtctacaaggtgtttggtctttatttcatgcagtcatacataggagtcttctaccatgcccttatacaccggaactttttgactcttcgtacagtcttgattcagcgcctccttgtgtctgaggtattggaaaacttggtggaaaacactttaccatatttgaagtacagttataagaagttcagagttcgaagcaagaagaaacgcgaaagaggatcatcaacagggaggatccactttgcttctagggtggaaaaggagtacttgaagccttcttattctgcaagcattggtgtggaacttgaagatgggcttttcgatgactttctagagttagcattgcagtttggaatgatcatgatgtttgcttgtgccttcccccctgcttttgcctttgcggctgtgaacaacataactgaaattaggacagatgcattgaagctgcttgtgatgcataagaggccaattcctcgtgtttccacaacaattggtgcttggctgaacatatttcagtttcttgttgttacatccatatgcaccaactgcgcccttctagtatggttatatgatcatgaggggaaatggaatatagagcctggcctagctgcaattctggttatggaacatatcctcctgttgattaagtttggtttctctcgccttgtacccgaggaacctgcttgggtacgggcgaaccgtgtgaaaagagcaacagaggccgagtacatgtgctccaaacagcttttgaggaatatttctggagatagggcagctggtactagtgcagttaagaaggaagaatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Anoctamin

Protein Analysis

692

Amino Acids

80.28

Weight (kDa)

9.17

Isoelectric Point (pI)

44.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Anoctamin PF04547 221 - 660 2.8e-100 Calcium-activated chloride channel
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 812, 1449
AasI GACNNNNNNGTC 1 cut(s) 1274
Acc36I ACCTGC 1 cut(s) 1960
AccI GTMKAC 2 cut(s) 716, 1278
AccII CGCG 1 cut(s) 1482
AciI CCGC 2 cut(s) 206, 1673
AclWI GGATC 3 cut(s) 1498, 1501, 1514
AcsI RAATTY 2 cut(s) 24, 110
AcuI CTGAAG 1 cut(s) 1073
AfaI GTAC 9 cut(s) 679, 1124, 1366, 1442, 1538, 1941, 1962, 2000, 2053
AfiI CCNNNNNNNGG 1 cut(s) 849
AflII CTTAAG 1 cut(s) 533
AflIII ACRYGT 1 cut(s) 2001
AgsI TTSAA 7 cut(s) 265, 359, 1016, 1438, 1543, 1580, 1714
AhlI ACTAGT 1 cut(s) 2054
AjnI CCWGG 3 cut(s) 145, 912, 1870
AjuI GAANNNNNNNTTGG 2 cut(s) 110, 142
AloI GAACNNNNNNTCC 2 cut(s) 1890, 1922
Alw21I GWGCWC 2 cut(s) 798, 2009
AlwI GGATC 3 cut(s) 1498, 1501, 1514
AlwNI CAGNNNCTG 1 cut(s) 790
Ama87I CYCGRG 1 cut(s) 1943
AoxI GGCC 3 cut(s) 1736, 1873, 1992
ApoI RAATTY 2 cut(s) 24, 110
Asp700I GAANNNNTTC 4 cut(s) 24, 503, 570, 1011
AspLEI GCGC 4 cut(s) 181, 280, 1383, 1823
AsuII TTCGAA 1 cut(s) 1465
AvaI CYCGRG 1 cut(s) 1943
BamHI GGATCC 1 cut(s) 1506
BanII GRGCYC 1 cut(s) 622
BauI CACGAG 1 cut(s) 1746
BbsI GAAGAC 1 cut(s) 1315
Bbv12I GWGCWC 2 cut(s) 798, 2009
BccI CCATC 6 cut(s) 337, 389, 488, 698, 876, 1577
BceAI ACGGC 1 cut(s) 1081
BcgI CGANNNNNNTGC 2 cut(s) 1934, 1968
BciT130I CCWGG 3 cut(s) 147, 914, 1872
BclI TGATCA 2 cut(s) 1629, 1843
BcuI ACTAGT 1 cut(s) 2054
BfaI CTAG 9 cut(s) 251, 477, 879, 1202, 1521, 1604, 1829, 1877, 2055
BfmI CTRYAG 3 cut(s) 433, 758, 960
BfoI RGCGCY 3 cut(s) 182, 281, 1384
BfrI CTTAAG 1 cut(s) 533
BfuAI ACCTGC 1 cut(s) 1960
BglI GCCNNNNNGGC 1 cut(s) 1673
BglII AGATCT 1 cut(s) 1212
BmcAI AGTACT 1 cut(s) 1538
Bme1390I CCNGG 3 cut(s) 147, 914, 1872
BmeT110I CYCGRG 1 cut(s) 1943
BmiI GGNNCC 3 cut(s) 51, 1508, 1950
BmrFI CCNGG 3 cut(s) 147, 914, 1872
BmrI ACTGGG 1 cut(s) 838
BmsI GCATC 4 cut(s) 364, 1048, 1696, 1716
BmuI ACTGGG 1 cut(s) 838
BpiI GAAGAC 1 cut(s) 1315
BplI GAGNNNNNCTC 2 cut(s) 650, 682
BpmI CTGGAG 1 cut(s) 2055
Bpu14I TTCGAA 1 cut(s) 1465
BsaBI GATNNNNATC 1 cut(s) 689
BsaJI CCNNGG 3 cut(s) 145, 913, 1944
BsaWI WCCGGW 1 cut(s) 1344
BsaXI ACNNNNNCTCC 6 cut(s) 780, 810, 1890, 1920, 1991, 2021
Bsc4I CCNNNNNNNGG 1 cut(s) 849
Bse1I ACTGG 3 cut(s) 632, 795, 844
Bse3DI GCAATG 2 cut(s) 427, 1613
Bse8I GATNNNNATC 1 cut(s) 689
BseBI CCWGG 3 cut(s) 147, 914, 1872
BseDI CCNNGG 3 cut(s) 145, 913, 1944
BseGI GGATG 2 cut(s) 348, 1802
BseJI GATNNNNATC 1 cut(s) 689
BseLI CCNNNNNNNGG 1 cut(s) 849
BseMI GCAATG 2 cut(s) 427, 1613
BseMII CTCAG 3 cut(s) 476, 1034, 1386
BseNI ACTGG 3 cut(s) 632, 795, 844
BseRI GAGGAG 2 cut(s) 29, 380
BseYI CCCAGC 1 cut(s) 928
BsgI GTGCAG 2 cut(s) 608, 2079
Bsh1236I CGCG 1 cut(s) 1482
BshFI GGCC 3 cut(s) 1738, 1875, 1994
BsiHKAI GWGCWC 2 cut(s) 798, 2009
BsiHKCI CYCGRG 1 cut(s) 1943
BsiSI CCGG 1 cut(s) 1345
BslFI GGGAC 3 cut(s) 128, 647, 997
BslI CCNNNNNNNGG 1 cut(s) 849
BsmFI GGGAC 3 cut(s) 128, 647, 997
BsnI GGCC 3 cut(s) 1738, 1875, 1994
BsoBI CYCGRG 1 cut(s) 1943
Bsp119I TTCGAA 1 cut(s) 1465
Bsp1286I GDGCHC 3 cut(s) 622, 798, 2009
Bsp143I GATC 7 cut(s) 247, 888, 1212, 1490, 1506, 1629, 1843
BspACI CCGC 2 cut(s) 206, 1673
BspANI GGCC 3 cut(s) 1738, 1875, 1994
BspCNI CTCAG 3 cut(s) 477, 1035, 1387
BspFNI CGCG 1 cut(s) 1482
BspHI TCATGA 3 cut(s) 969, 1632, 1846
BspLI GGNNCC 3 cut(s) 51, 1508, 1950
BspMAI CTGCAG 1 cut(s) 762
BspMI ACCTGC 1 cut(s) 1960
BspPI GGATC 3 cut(s) 1498, 1501, 1514
BspT104I TTCGAA 1 cut(s) 1465
BspTI CTTAAG 1 cut(s) 533
BsrDI GCAATG 2 cut(s) 427, 1613
BsrI ACTGG 3 cut(s) 632, 795, 844
BssECI CCNNGG 3 cut(s) 145, 913, 1944
BssMI GATC 7 cut(s) 247, 888, 1212, 1490, 1506, 1629, 1843
BssNAI GTATAC 1 cut(s) 717
BssSI CACGAG 1 cut(s) 1746
Bst1107I GTATAC 1 cut(s) 717
Bst2BI CACGAG 1 cut(s) 1746
Bst2UI CCWGG 3 cut(s) 147, 914, 1872
Bst4CI ACNGT 5 cut(s) 89, 723, 1369, 1445, 1973
Bst6I CTCTTC 1 cut(s) 1365
BstAFI CTTAAG 1 cut(s) 533
BstAPI GCANNNNNTGC 1 cut(s) 1818
BstBI TTCGAA 1 cut(s) 1465
BstC8I GCNNGC 5 cut(s) 174, 273, 647, 1107, 1561
BstDEI CTNAG 3 cut(s) 485, 1043, 1395
BstF5I GGATG 2 cut(s) 348, 1802
BstFNI CGCG 1 cut(s) 1482
BstH2I RGCGCY 3 cut(s) 182, 281, 1384
BstHHI GCGC 4 cut(s) 181, 280, 1383, 1823
BstKTI GATC 7 cut(s) 250, 891, 1215, 1493, 1509, 1632, 1846
BstMBI GATC 7 cut(s) 247, 888, 1212, 1490, 1506, 1629, 1843
BstMWI GCNNNNNNNGC 8 cut(s) 178, 277, 1102, 1673, 1726, 1735, 1818, 1991
BstNI CCWGG 3 cut(s) 147, 914, 1872
BstNSI RCATGY 1 cut(s) 2005
BstSCI CCNGG 3 cut(s) 145, 912, 1870
BstSFI CTRYAG 3 cut(s) 433, 758, 960
BstUI CGCG 1 cut(s) 1482
BstV2I GAAGAC 1 cut(s) 1315
BstX2I RGATCY 2 cut(s) 1212, 1506
BstYI RGATCY 2 cut(s) 1212, 1506
BstZ17I GTATAC 1 cut(s) 717
BsuRI GGCC 3 cut(s) 1738, 1875, 1994
BtsCI GGATG 2 cut(s) 348, 1802
BtsIMutI CAGTG 1 cut(s) 625
BveI ACCTGC 1 cut(s) 1960
Cac8I GCNNGC 5 cut(s) 174, 273, 647, 1107, 1561
CaiI CAGNNNCTG 1 cut(s) 790
CciI TCATGA 3 cut(s) 969, 1632, 1846
CfoI GCGC 4 cut(s) 181, 280, 1383, 1823
Csp6I GTAC 9 cut(s) 678, 1123, 1365, 1441, 1537, 1940, 1961, 1999, 2052
CspCI CAANNNNNGTGG 2 cut(s) 1744, 1779
CviAII CATG 8 cut(s) 581, 934, 970, 1303, 1331, 1633, 1847, 2002
CviQI GTAC 9 cut(s) 678, 1123, 1365, 1441, 1537, 1940, 1961, 1999, 2052
DdeI CTNAG 3 cut(s) 485, 1043, 1395
DpnI GATC 7 cut(s) 249, 890, 1214, 1492, 1508, 1631, 1845
DpnII GATC 7 cut(s) 247, 888, 1212, 1490, 1506, 1629, 1843
DrdI GACNNNNNNGTC 1 cut(s) 1274
DseDI GACNNNNNNGTC 1 cut(s) 1274
Eam1104I CTCTTC 1 cut(s) 1365
EarI CTCTTC 1 cut(s) 1365
Eco24I GRGCYC 1 cut(s) 622
Eco57I CTGAAG 1 cut(s) 1073
Eco88I CYCGRG 1 cut(s) 1943
EcoRII CCWGG 3 cut(s) 145, 912, 1870
EcoT22I ATGCAT 2 cut(s) 1711, 1731
EcoT38I GRGCYC 1 cut(s) 622
FaeI CATG 8 cut(s) 584, 937, 973, 1306, 1334, 1636, 1850, 2005
FalI AAGNNNNNCTT 4 cut(s) 1523, 1555, 1572, 1604
FaqI GGGAC 3 cut(s) 128, 647, 997
FatI CATG 8 cut(s) 580, 933, 969, 1302, 1330, 1632, 1846, 2001
FauNDI CATATG 2 cut(s) 1111, 1808
FbaI TGATCA 2 cut(s) 1629, 1843
FblI GTMKAC 2 cut(s) 716, 1278
FokI GGATG 2 cut(s) 355, 1789
FriOI GRGCYC 1 cut(s) 622
FspBI CTAG 9 cut(s) 251, 477, 879, 1202, 1521, 1604, 1829, 1877, 2055
GlaI GCGC 4 cut(s) 180, 279, 1382, 1822
GsaI CCCAGC 1 cut(s) 932
GsuI CTGGAG 1 cut(s) 2055
HaeII RGCGCY 3 cut(s) 182, 281, 1384
HaeIII GGCC 3 cut(s) 1738, 1875, 1994
HapII CCGG 1 cut(s) 1345
HhaI GCGC 4 cut(s) 181, 280, 1383, 1823
Hin1II CATG 8 cut(s) 584, 937, 973, 1306, 1334, 1636, 1850, 2005
Hin6I GCGC 4 cut(s) 179, 278, 1381, 1821
HinP1I GCGC 4 cut(s) 179, 278, 1381, 1821
HincII GTYRAC 2 cut(s) 876, 1161
HindII GTYRAC 2 cut(s) 876, 1161
HindIII AAGCTT 1 cut(s) 1223
HinfI GANTC 6 cut(s) 141, 545, 1047, 1320, 1357, 1375
HpaI GTTAAC 1 cut(s) 876
HpaII CCGG 1 cut(s) 1345
Hpy166II GTNNAC 5 cut(s) 717, 876, 1161, 1279, 1681
Hpy188I TCNGA 5 cut(s) 486, 1052, 1141, 1396, 1460
Hpy188III TCNNGA 8 cut(s) 521, 850, 970, 1372, 1604, 1633, 1847, 2034
Hpy8I GTNNAC 5 cut(s) 717, 876, 1161, 1279, 1681
HpyAV CCTTC 8 cut(s) 193, 292, 617, 1097, 1557, 1660, 1835, 2062
HpyCH4III ACNGT 5 cut(s) 89, 723, 1369, 1445, 1973
HpyF10VI GCNNNNNNNGC 8 cut(s) 178, 277, 1102, 1673, 1726, 1735, 1818, 1991
HpyF3I CTNAG 3 cut(s) 485, 1043, 1395
Hsp92II CATG 8 cut(s) 584, 937, 973, 1306, 1334, 1636, 1850, 2005
HspAI GCGC 4 cut(s) 179, 278, 1381, 1821
Ksp22I TGATCA 2 cut(s) 1629, 1843
KspAI GTTAAC 1 cut(s) 876
Kzo9I GATC 7 cut(s) 247, 888, 1212, 1490, 1506, 1629, 1843
LmnI GCTCC 3 cut(s) 262, 801, 2012
LweI GCATC 4 cut(s) 364, 1048, 1696, 1716
MaeI CTAG 9 cut(s) 251, 477, 879, 1202, 1521, 1604, 1829, 1877, 2055
MaeIII GTNAC 3 cut(s) 546, 786, 1798
MalI GATC 7 cut(s) 249, 890, 1214, 1492, 1508, 1631, 1845
MboI GATC 7 cut(s) 247, 888, 1212, 1490, 1506, 1629, 1843
MboII GAAGA 8 cut(s) 89, 92, 610, 985, 1315, 1352, 1486, 1592
MfeI CAATTG 1 cut(s) 1761
MflI RGATCY 2 cut(s) 1212, 1506
MhlI GDGCHC 3 cut(s) 622, 798, 2009
MlyI GAGTC 4 cut(s) 150, 554, 1329, 1351
Mph1103I ATGCAT 2 cut(s) 1711, 1731
MroXI GAANNNNTTC 4 cut(s) 24, 503, 570, 1011
MslI CAYNNNNRTG 2 cut(s) 1568, 1807
MspA1I CMGCKG 3 cut(s) 411, 928, 2048
MspCI CTTAAG 1 cut(s) 533
MspI CCGG 1 cut(s) 1345
MspR9I CCNGG 3 cut(s) 147, 914, 1872
MunI CAATTG 1 cut(s) 1761
MvaI CCWGG 3 cut(s) 147, 914, 1872
MvnI CGCG 1 cut(s) 1482
MwoI GCNNNNNNNGC 8 cut(s) 178, 277, 1102, 1673, 1726, 1735, 1818, 1991
NdeI CATATG 2 cut(s) 1111, 1808
NdeII GATC 7 cut(s) 247, 888, 1212, 1490, 1506, 1629, 1843
NlaIII CATG 8 cut(s) 584, 937, 973, 1306, 1334, 1636, 1850, 2005
NlaIV GGNNCC 3 cut(s) 51, 1508, 1950
NmeAIII GCCGAG 1 cut(s) 2020
NmuCI GTSAC 1 cut(s) 546
NsiI ATGCAT 2 cut(s) 1711, 1731
NspI RCATGY 1 cut(s) 2005
NspV TTCGAA 1 cut(s) 1465
PagI TCATGA 3 cut(s) 969, 1632, 1846
PciI ACATGT 1 cut(s) 2001
PcsI WCGNNNNNNNCGW 1 cut(s) 1969
PdmI GAANNNNTTC 4 cut(s) 24, 503, 570, 1011
PfeI GAWTC 2 cut(s) 1047, 1375
PleI GAGTC 4 cut(s) 149, 553, 1328, 1351
PpsI GAGTC 4 cut(s) 149, 553, 1328, 1351
PscI ACATGT 1 cut(s) 2001
PsiI TTATAA 2 cut(s) 812, 1449
Psp6I CCWGG 3 cut(s) 145, 912, 1870
PspFI CCCAGC 1 cut(s) 928
PspGI CCWGG 3 cut(s) 145, 912, 1870
PspN4I GGNNCC 3 cut(s) 51, 1508, 1950
PstI CTGCAG 1 cut(s) 762
PstNI CAGNNNCTG 1 cut(s) 790
PsuI RGATCY 2 cut(s) 1212, 1506
PvuII CAGCTG 3 cut(s) 411, 928, 2048
RsaI GTAC 9 cut(s) 679, 1124, 1366, 1442, 1538, 1941, 1962, 2000, 2053
RsaNI GTAC 9 cut(s) 678, 1123, 1365, 1441, 1537, 1940, 1961, 1999, 2052
RseI CAYNNNNRTG 2 cut(s) 1568, 1807
Sau3AI GATC 7 cut(s) 247, 888, 1212, 1490, 1506, 1629, 1843
ScaI AGTACT 1 cut(s) 1538
SchI GAGTC 4 cut(s) 150, 554, 1329, 1351
ScrFI CCNGG 3 cut(s) 147, 914, 1872
SduI GDGCHC 3 cut(s) 622, 798, 2009
SfaNI GCATC 4 cut(s) 364, 1048, 1696, 1716
SfcI CTRYAG 3 cut(s) 433, 758, 960
SfuI TTCGAA 1 cut(s) 1465
SmiMI CAYNNNNRTG 2 cut(s) 1568, 1807
SmlI CTYRAG 1 cut(s) 533
SmoI CTYRAG 1 cut(s) 533
SpeI ACTAGT 1 cut(s) 2054
SsiI CCGC 2 cut(s) 206, 1673
SspI AATATT 1 cut(s) 2029
SspMI CTAG 9 cut(s) 251, 477, 879, 1202, 1521, 1604, 1829, 1877, 2055
StyD4I CCNGG 3 cut(s) 145, 912, 1870
TaaI ACNGT 5 cut(s) 89, 723, 1369, 1445, 1973
TaqI TCGA 3 cut(s) 156, 1465, 1593
TatI WGTACW 3 cut(s) 1440, 1536, 1998
TauI GCSGC 1 cut(s) 1676
TfiI GAWTC 2 cut(s) 1047, 1375
TscAI CASTG 1 cut(s) 632
TseFI GTSAC 1 cut(s) 546
Tsp45I GTSAC 1 cut(s) 546
TspDTI ATGAA 9 cut(s) 17, 123, 390, 416, 473, 600, 986, 1260, 1291
TspRI CASTG 1 cut(s) 632
Vha464I CTTAAG 1 cut(s) 533
XapI RAATTY 2 cut(s) 24, 110
XbaI TCTAGA 1 cut(s) 1603
XceI RCATGY 1 cut(s) 2005
XmiI GTMKAC 2 cut(s) 716, 1278
XmnI GAANNNNTTC 4 cut(s) 24, 503, 570, 1011
XspI CTAG 9 cut(s) 251, 477, 879, 1202, 1521, 1604, 1829, 1877, 2055
ZrmI AGTACT 1 cut(s) 1538
Zsp2I ATGCAT 2 cut(s) 1711, 1731
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.