Basic Information

Type: gene

Biological Identity

Species

rosa_multiflora

Chromosome

Rmu_sc0001277.1

Orthogroup ID

OG0006685

Physical Location & Seq

Genomic Coordinates Forward (+)

7056 .. 11860

Gene Structure 4805 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rmu_sc0001277.1_g000002.1.cds

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1524 bp

atggcctcgccttcccctggagataagcacagctctgtgaaaagagtggctgttgttggcgctggtgttagtgggcttgctgcggcctacaagttgaaatcacatggtttccacgttacggtactcgaagccgagggaagagctggagggaagttgagaagtgtgtcgtgtaatggtcttgtttgggatgaaggtgcaaatacaatgactgagagtgaagctgaggttcaaactttgcttgataatctggggcttcgagaaaagcagcaatttccgatttcacagaacaaacggtatgttgcaaggaatgggtcgcctgtgctgctacctaccaatccgattgagctgatcaagagcaactttctttctacaaaatcaaagtttcagattcttctggagccatatttgtggaagaaaaaaagagtttccgatgatcacacccgagaaactgtgggtgggttctttcagcgtcattttgggaaagaggtcgttgattatctcattgatccctttgttgccgggacaagtgctggagatcctgaatctctttcgatgccacattcttttccagagttatggaacatagaaaaaaggtatggttcggttatagctggggcaattcagtcaaaattatcttccaggaaggaaaaaggtagagaaacaaagggttctgtagagaaaggaaagcgtcagcgcggttctttttcctttcagggtggtatgcagacactcactgatacattgtgcaaacagcttggcaaacatgagcttaaactgaactcaaaggttttgtcattatcttacagtcatgatgggaacaccacatcagaaaattggtcggtttcctgtgctgctaataatgacaagcatttacaaagttcatctgttgatgctataatcctgacggctccactctgcagtatcaaagaaatgaagatctcgagaagaggaaccatcttcccacttgattttcttcctgaggtgacttatatgccgctatcagtgattataaccagcttcaagaaggaaaacgttaaaaggcctcttgagggatttggagttcttgttccctccaaagagcaggaaaatggcttaaaaacgctaggtacactattttcttccatgatgtttccagatcgtgcacctagtgaccaatatctttacactacctttgttgggggaagtcgaaacaaggaactggcaaaagcttcgaaggatgagttgaaggaaattgtttcctctgacataaggcaacttttgggagcggaaggcgagccgacatttgtgaaccattaccattggagcaaagcatttccattgtatgggcataactacgattcagtcattgaagcaatcgagcagatggagaaaaatcttcctggtttattctatgcaggtaaccataggggtggactatctgttggcaaagcaatagcttcggggtgcaaagcagctgaacttgcaatctcctatctggaatcttcttcagatggcaagatgcttcagcaaggatcgtcatcttag

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0004729 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005740 GO:0005743 GO:0006725 GO:0006778 GO:0006779 GO:0006783 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009507 GO:0009526 GO:0009528 GO:0009536 GO:0009706 GO:0009790 GO:0009791 GO:0009793 GO:0009941 GO:0009987 GO:0010154 GO:0015994 GO:0015995 GO:0016020 GO:0016491 GO:0016627 GO:0016634 GO:0018130 GO:0019438 GO:0019866 GO:0022414 GO:0031090 GO:0031966 GO:0031967 GO:0031969 GO:0031975 GO:0032501 GO:0032502 GO:0033013 GO:0033014 GO:0034641 GO:0042168 GO:0042170 GO:0042440 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044249 GO:0044271 GO:0044422 GO:0044424 GO:0044429 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046148 GO:0046483 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0051186 GO:0051188 GO:0055114 GO:0061458 GO:0070818 GO:0071704 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00860 ko01100 ko01110 map00860 map01100 map01110

Pfam Domains

Protein Families

Amino_oxidase

Protein Analysis

507

Amino Acids

55.57

Weight (kDa)

9.03

Isoelectric Point (pI)

47.13

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0006685)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G14220 AT5G14220 AT5G14220 AT5G14220 AT5G14220
fragaria_vesca	FvH4_6g53090 FvH4_6g53090 FvH4_6g53090
malus_domestica	MD17G1014700.v1.1 MD17G1014800.v1.1
prunus_persica	Prupe.3G303000_v2.0.a1 Prupe.3G303000_v2.0.a1 Prupe.3G303000_v2.0.a1
pyrus_communis	pycom17g01180
rosa_chinensis	RchiOBHm_Chr2g0174871 RchiOBHm_Chr2g0174941
rosa_laevigata	RLG00000022303
rosa_multiflora	Rmu_sc0001277.1_g000002 Rmu_sc0009766.1_g000003
rosa_roxburghii	Rroxscaffold_2G00077620
rosa_rugosa	Rorug02G0580300
rosa_samantha	Rh2AG661400 Rh2BG672700 Rh2CG635500 Rh2DG685900
rosa_wichuraiana	Rw2G054200

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1010
Acc36I	ACCTGC	1 cut(s)	1385
AccB7I	CCANNNNNTGG	1 cut(s)	1322
AccBSI	CCGCTC	1 cut(s)	1265
AccII	CGCG	1 cut(s)	696
AciI	CCGC	4 cut(s)	83, 696, 995, 1265
AclI	AACGTT	1 cut(s)	1032
AclWI	GGATC	3 cut(s)	500, 530, 1519
AcuI	CTGAAG	2 cut(s)	1470, 1487
AdeI	CACNNNGTG	1 cut(s)	1148
AfaI	GTAC	2 cut(s)	123, 1108
AfiI	CCNNNNNNNGG	5 cut(s)	17, 118, 1049, 1176, 1322
AgsI	TTSAA	5 cut(s)	97, 230, 1021, 1225, 1349
AjnI	CCWGG	3 cut(s)	16, 638, 1378
AloI	GAACNNNNNNTCC	4 cut(s)	1044, 1050, 1076, 1082
Alw21I	GWGCWC	1 cut(s)	1144
Alw44I	GTGCAC	1 cut(s)	1140
AlwI	GGATC	3 cut(s)	500, 530, 1519
Ama87I	CYCGRG	2 cut(s)	441, 940
AoxI	GGCC	3 cut(s)	3, 84, 1040
ApaLI	GTGCAC	1 cut(s)	1140
ApeKI	GCWGC	5 cut(s)	80, 265, 322, 851, 1451
ArsI	GACNNNNNNTTYG	2 cut(s)	776, 808
AspLEI	GCGC	2 cut(s)	62, 696
AsuC2I	CCSGG	1 cut(s)	520
AsuHPI	GGTGA	1 cut(s)	994
AsuII	TTCGAA	1 cut(s)	1211
AvaI	CYCGRG	2 cut(s)	441, 940
AxyI	CCTNAGG	1 cut(s)	978
BaeGI	GKGCMC	1 cut(s)	1144
Bbv12I	GWGCWC	1 cut(s)	1144
BbvCI	CCTCAGC	1 cut(s)	222
BbvI	GCAGC	5 cut(s)	67, 277, 309, 838, 1463
BccI	CCATC	4 cut(s)	806, 962, 1357, 1484
BceAI	ACGGC	1 cut(s)	921
BcgI	CGANNNNNNTGC	4 cut(s)	1191, 1225, 1419, 1453
BciT130I	CCWGG	3 cut(s)	18, 640, 1380
BclI	TGATCA	2 cut(s)	348, 433
BcnI	CCSGG	1 cut(s)	520
BfaI	CTAG	2 cut(s)	1103, 1146
BfmI	CTRYAG	2 cut(s)	672, 916
BfoI	RGCGCY	1 cut(s)	63
BfuAI	ACCTGC	1 cut(s)	1385
BglII	AGATCT	1 cut(s)	936
BisI	GCNGC	7 cut(s)	81, 84, 266, 323, 852, 995, 1452
BlsI	GCNGC	7 cut(s)	82, 85, 267, 324, 853, 996, 1453
Bme1390I	CCNGG	4 cut(s)	18, 520, 640, 1380
BmeT110I	CYCGRG	2 cut(s)	441, 940
BmiI	GGNNCC	3 cut(s)	399, 909, 952
BmrFI	CCNGG	4 cut(s)	18, 520, 640, 1380
BmsI	GCATC	3 cut(s)	543, 880, 1488
BpmI	CTGGAG	4 cut(s)	39, 165, 416, 552
Bpu10I	CCTNAGC	1 cut(s)	222
Bpu14I	TTCGAA	1 cut(s)	1211
BpuEI	CTTGAG	1 cut(s)	1067
BpuMI	CCSGG	1 cut(s)	520
BsaBI	GATNNNNATC	1 cut(s)	1516
BsaJI	CCNNGG	2 cut(s)	16, 132
BsaXI	ACNNNNNCTCC	2 cut(s)	1050, 1080
Bsc4I	CCNNNNNNNGG	5 cut(s)	17, 118, 1049, 1176, 1322
Bse1I	ACTGG	1 cut(s)	1203
Bse21I	CCTNAGG	1 cut(s)	978
Bse8I	GATNNNNATC	1 cut(s)	1516
BseBI	CCWGG	3 cut(s)	18, 640, 1380
BseDI	CCNNGG	2 cut(s)	16, 132
BseGI	GGATG	2 cut(s)	193, 1222
BseJI	GATNNNNATC	1 cut(s)	1516
BseLI	CCNNNNNNNGG	5 cut(s)	17, 118, 1049, 1176, 1322
BseMII	CTCAG	3 cut(s)	201, 213, 969
BseNI	ACTGG	1 cut(s)	1203
BseSI	GKGCMC	1 cut(s)	1144
BseXI	GCAGC	5 cut(s)	67, 277, 309, 838, 1463
BseYI	CCCAGC	1 cut(s)	611
Bsh1236I	CGCG	1 cut(s)	696
BshFI	GGCC	3 cut(s)	5, 86, 1042
BsiHKAI	GWGCWC	1 cut(s)	1144
BsiHKCI	CYCGRG	2 cut(s)	441, 940
BsiSI	CCGG	1 cut(s)	519
BslFI	GGGAC	1 cut(s)	535
BslI	CCNNNNNNNGG	5 cut(s)	17, 118, 1049, 1176, 1322
BsmFI	GGGAC	1 cut(s)	535
BsnI	GGCC	3 cut(s)	5, 86, 1042
BsoBI	CYCGRG	2 cut(s)	441, 940
Bsp119I	TTCGAA	1 cut(s)	1211
Bsp1286I	GDGCHC	1 cut(s)	1144
Bsp143I	GATC	7 cut(s)	348, 433, 505, 535, 936, 1135, 1511
BspACI	CCGC	4 cut(s)	83, 696, 995, 1265
BspANI	GGCC	3 cut(s)	5, 86, 1042
BspCNI	CTCAG	3 cut(s)	202, 214, 970
BspFNI	CGCG	1 cut(s)	696
BspHI	TCATGA	1 cut(s)	808
BspLI	GGNNCC	3 cut(s)	399, 909, 952
BspMAI	CTGCAG	1 cut(s)	920
BspMI	ACCTGC	1 cut(s)	1385
BspPI	GGATC	3 cut(s)	500, 530, 1519
BspQI	GCTCTTC	1 cut(s)	133
BspT104I	TTCGAA	1 cut(s)	1211
BsrBI	CCGCTC	1 cut(s)	1265
BsrI	ACTGG	1 cut(s)	1203
BssECI	CCNNGG	2 cut(s)	16, 132
BssMI	GATC	7 cut(s)	348, 433, 505, 535, 936, 1135, 1511
Bst2UI	CCWGG	3 cut(s)	18, 640, 1380
Bst4CI	ACNGT	4 cut(s)	121, 294, 451, 806
Bst6I	CTCTTC	2 cut(s)	133, 940
BstBI	TTCGAA	1 cut(s)	1211
BstC8I	GCNNGC	2 cut(s)	78, 1274
BstDEI	CTNAG	4 cut(s)	210, 222, 978, 1521
BstEII	GGTNACC	1 cut(s)	1397
BstF5I	GGATG	2 cut(s)	193, 1222
BstFNI	CGCG	1 cut(s)	696
BstH2I	RGCGCY	1 cut(s)	63
BstHHI	GCGC	2 cut(s)	62, 696
BstKTI	GATC	7 cut(s)	351, 436, 508, 538, 939, 1138, 1514
BstMBI	GATC	7 cut(s)	348, 433, 505, 535, 936, 1135, 1511
BstMWI	GCNNNNNNNGC	2 cut(s)	322, 1460
BstNI	CCWGG	3 cut(s)	18, 640, 1380
BstPI	GGTNACC	1 cut(s)	1397
BstSCI	CCNGG	4 cut(s)	16, 518, 638, 1378
BstSFI	CTRYAG	2 cut(s)	672, 916
BstSLI	GKGCMC	1 cut(s)	1144
BstUI	CGCG	1 cut(s)	696
BstV1I	GCAGC	5 cut(s)	67, 277, 309, 838, 1463
BstX2I	RGATCY	2 cut(s)	535, 936
BstXI	CCANNNNNNTGG	3 cut(s)	408, 576, 1409
BstYI	RGATCY	2 cut(s)	535, 936
Bsu36I	CCTNAGG	1 cut(s)	978
BsuRI	GGCC	3 cut(s)	5, 86, 1042
BtsCI	GGATG	2 cut(s)	193, 1222
BtsIMutI	CAGTG	2 cut(s)	732, 1008
BveI	ACCTGC	1 cut(s)	1385
Cac8I	GCNNGC	2 cut(s)	78, 1274
CciI	TCATGA	1 cut(s)	808
CfoI	GCGC	2 cut(s)	62, 696
CseI	GACGC	2 cut(s)	458, 677
Csp6I	GTAC	2 cut(s)	122, 1107
CviAII	CATG	4 cut(s)	104, 764, 809, 1123
CviQI	GTAC	2 cut(s)	122, 1107
DdeI	CTNAG	4 cut(s)	210, 222, 978, 1521
DpnI	GATC	7 cut(s)	350, 435, 507, 537, 938, 1137, 1513
DpnII	GATC	7 cut(s)	348, 433, 505, 535, 936, 1135, 1511
DraIII	CACNNNGTG	1 cut(s)	1148
Eam1104I	CTCTTC	2 cut(s)	133, 940
EarI	CTCTTC	2 cut(s)	133, 940
Eco147I	AGGCCT	1 cut(s)	1042
Eco57I	CTGAAG	2 cut(s)	1470, 1487
Eco81I	CCTNAGG	1 cut(s)	978
Eco88I	CYCGRG	2 cut(s)	441, 940
Eco91I	GGTNACC	1 cut(s)	1397
EcoO65I	GGTNACC	1 cut(s)	1397
EcoRII	CCWGG	3 cut(s)	16, 638, 1378
FaeI	CATG	4 cut(s)	107, 767, 812, 1126
FalI	AAGNNNNNCTT	2 cut(s)	344, 376
FaqI	GGGAC	1 cut(s)	535
FatI	CATG	4 cut(s)	103, 763, 808, 1122
FbaI	TGATCA	2 cut(s)	348, 433
Fnu4HI	GCNGC	7 cut(s)	81, 84, 266, 323, 852, 995, 1452
FokI	GGATG	2 cut(s)	200, 1229
Fsp4HI	GCNGC	7 cut(s)	81, 84, 266, 323, 852, 995, 1452
FspBI	CTAG	2 cut(s)	1103, 1146
GlaI	GCGC	2 cut(s)	61, 695
GluI	GCNGC	7 cut(s)	81, 84, 266, 323, 852, 995, 1452
GsaI	CCCAGC	1 cut(s)	615
GsuI	CTGGAG	4 cut(s)	39, 165, 416, 552
HaeII	RGCGCY	1 cut(s)	63
HaeIII	GGCC	3 cut(s)	5, 86, 1042
HapII	CCGG	1 cut(s)	519
HgaI	GACGC	2 cut(s)	458, 677
HhaI	GCGC	2 cut(s)	62, 696
Hin1II	CATG	4 cut(s)	107, 767, 812, 1126
Hin6I	GCGC	2 cut(s)	60, 694
HinP1I	GCGC	2 cut(s)	60, 694
HindIII	AAGCTT	1 cut(s)	1206
HinfI	GANTC	4 cut(s)	388, 542, 1337, 1478
HpaII	CCGG	1 cut(s)	519
HphI	GGTGA	1 cut(s)	994
Hpy166II	GTNNAC	4 cut(s)	1109, 1142, 1288, 1412
Hpy188I	TCNGA	7 cut(s)	276, 339, 387, 430, 829, 1243, 1489
Hpy8I	GTNNAC	4 cut(s)	1109, 1142, 1288, 1412
HpyAV	CCTTC	7 cut(s)	21, 185, 637, 1018, 1207, 1219, 1262
HpyCH4III	ACNGT	4 cut(s)	121, 294, 451, 806
HpyCH4IV	ACGT	2 cut(s)	114, 1032
HpyCH4V	TGCA	9 cut(s)	197, 302, 724, 747, 918, 1142, 1394, 1446, 1463
HpyF10VI	GCNNNNNNNGC	2 cut(s)	322, 1460
HpyF3I	CTNAG	4 cut(s)	210, 222, 978, 1521
HpySE526I	ACGT	2 cut(s)	114, 1032
Hsp92II	CATG	4 cut(s)	107, 767, 812, 1126
HspAI	GCGC	2 cut(s)	60, 694
Ksp22I	TGATCA	2 cut(s)	348, 433
Kzo9I	GATC	7 cut(s)	348, 433, 505, 535, 936, 1135, 1511
LguI	GCTCTTC	1 cut(s)	133
LmnI	GCTCC	4 cut(s)	397, 913, 1262, 1302
Lsp1109I	GCAGC	5 cut(s)	67, 277, 309, 838, 1463
LweI	GCATC	3 cut(s)	543, 880, 1488
MaeI	CTAG	2 cut(s)	1103, 1146
MaeII	ACGT	2 cut(s)	114, 1032
MaeIII	GTNAC	4 cut(s)	115, 982, 1148, 1397
MalI	GATC	7 cut(s)	350, 435, 507, 537, 938, 1137, 1513
MbiI	CCGCTC	1 cut(s)	1265
MboI	GATC	7 cut(s)	348, 433, 505, 535, 936, 1135, 1511
MflI	RGATCY	2 cut(s)	535, 936
MhlI	GDGCHC	1 cut(s)	1144
MluCI	AATT	5 cut(s)	269, 618, 629, 832, 1230
MseI	TTAA	3 cut(s)	771, 1035, 1094
MslI	CAYNNNNRTG	2 cut(s)	406, 1407
MspA1I	CMGCKG	1 cut(s)	1454
MspI	CCGG	1 cut(s)	519
MspR9I	CCNGG	4 cut(s)	18, 520, 640, 1380
MvaI	CCWGG	3 cut(s)	18, 640, 1380
MvnI	CGCG	1 cut(s)	696
MwoI	GCNNNNNNNGC	2 cut(s)	322, 1460
NciI	CCSGG	1 cut(s)	520
NdeII	GATC	7 cut(s)	348, 433, 505, 535, 936, 1135, 1511
NlaIII	CATG	4 cut(s)	107, 767, 812, 1126
NlaIV	GGNNCC	3 cut(s)	399, 909, 952
NmeAIII	GCCGAG	1 cut(s)	157
NmuCI	GTSAC	2 cut(s)	982, 1148
NspV	TTCGAA	1 cut(s)	1211
PaeR7I	CTCGAG	1 cut(s)	940
PagI	TCATGA	1 cut(s)	808
PceI	AGGCCT	1 cut(s)	1042
PciSI	GCTCTTC	1 cut(s)	133
PfeI	GAWTC	4 cut(s)	388, 542, 1337, 1478
PflMI	CCANNNNNTGG	1 cut(s)	1322
PfoI	TCCNGGA	1 cut(s)	638
PkrI	GCNGC	7 cut(s)	82, 85, 267, 324, 853, 996, 1453
PsiI	TTATAA	1 cut(s)	1010
Psp1406I	AACGTT	1 cut(s)	1032
Psp6I	CCWGG	3 cut(s)	16, 638, 1378
PspEI	GGTNACC	1 cut(s)	1397
PspFI	CCCAGC	1 cut(s)	611
PspGI	CCWGG	3 cut(s)	16, 638, 1378
PspN4I	GGNNCC	3 cut(s)	399, 909, 952
PstI	CTGCAG	1 cut(s)	920
PsuI	RGATCY	2 cut(s)	535, 936
PvuII	CAGCTG	1 cut(s)	1454
RsaI	GTAC	2 cut(s)	123, 1108
RsaNI	GTAC	2 cut(s)	122, 1107
RseI	CAYNNNNRTG	2 cut(s)	406, 1407
SapI	GCTCTTC	1 cut(s)	133
SaqAI	TTAA	3 cut(s)	771, 1035, 1094
SatI	GCNGC	7 cut(s)	81, 84, 266, 323, 852, 995, 1452
Sau3AI	GATC	7 cut(s)	348, 433, 505, 535, 936, 1135, 1511
ScrFI	CCNGG	4 cut(s)	18, 520, 640, 1380
SduI	GDGCHC	1 cut(s)	1144
SfaNI	GCATC	3 cut(s)	543, 880, 1488
SfcI	CTRYAG	2 cut(s)	672, 916
Sfr274I	CTCGAG	1 cut(s)	940
SfuI	TTCGAA	1 cut(s)	1211
SlaI	CTCGAG	1 cut(s)	940
SmiMI	CAYNNNNRTG	2 cut(s)	406, 1407
SmlI	CTYRAG	2 cut(s)	940, 1046
SmoI	CTYRAG	2 cut(s)	940, 1046
Sse9I	AATT	5 cut(s)	269, 618, 629, 832, 1230
SseBI	AGGCCT	1 cut(s)	1042
SsiI	CCGC	4 cut(s)	83, 696, 995, 1265
SspMI	CTAG	2 cut(s)	1103, 1146
StuI	AGGCCT	1 cut(s)	1042
StyD4I	CCNGG	4 cut(s)	16, 518, 638, 1378
TaaI	ACNGT	4 cut(s)	121, 294, 451, 806
TaiI	ACGT	2 cut(s)	117, 1035
TaqI	TCGA	7 cut(s)	126, 256, 551, 941, 1186, 1211, 1356
TasI	AATT	5 cut(s)	269, 618, 629, 832, 1230
TauI	GCSGC	2 cut(s)	86, 997
TfiI	GAWTC	4 cut(s)	388, 542, 1337, 1478
Tru1I	TTAA	3 cut(s)	771, 1035, 1094
Tru9I	TTAA	3 cut(s)	771, 1035, 1094
TscAI	CASTG	2 cut(s)	739, 1008
TseFI	GTSAC	2 cut(s)	982, 1148
TseI	GCWGC	5 cut(s)	80, 265, 322, 851, 1451
Tsp45I	GTSAC	2 cut(s)	982, 1148
TspDTI	ATGAA	3 cut(s)	204, 870, 947
TspRI	CASTG	2 cut(s)	739, 1008
Van91I	CCANNNNNTGG	1 cut(s)	1322
VneI	GTGCAC	1 cut(s)	1140
XhoI	CTCGAG	1 cut(s)	940
XspI	CTAG	2 cut(s)	1103, 1146

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.