Rmu_sc0001460.1_g000014

PB1 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001460.1
OG0018125
Physical Location & Seq
Forward (+)
71509 .. 72324
816 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 468 bp
atggagaacaactcacctacaccagcgctcctaggggaagacctcgacgccttgatctccgtcaccaacgacgacaacctcgagcacatgatgcacgagtacgatagcctctactgggctttcgccaggccctccaggatggctgtttctcttcctgatcaatcacagcaacagcttcaggtcatcgggtcggatccggtagtgagcccagtggagttccagaagcaattgcaagacttgcagaggctgcaatttggagatcaaactgatcatcagtatcgaagaaagagtgataacaacctcatcggagcaggaggaggaagctacggtgccgccgtcggtgattactacatgcagaaggtggcgtatgatagcgtaacgggtattgaaaattggggaggaggtggtggggcagaagcaccggcgagaggaatgagggttgtgatggtggtggtgggttgtcattga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PB1

Protein Analysis

155

Amino Acids

16.95

Weight (kDa)

4.57

Isoelectric Point (pI)

57.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PB1 PF00564 10 - 40 9.2e-06 PB1 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 329
AciI CCGC 1 cut(s) 333
AclWI GGATC 2 cut(s) 188, 201
AcuI CTGAAG 1 cut(s) 161
AcyI GRCGYC 1 cut(s) 48
AfaI GTAC 1 cut(s) 101
AfeI AGCGCT 1 cut(s) 27
AfiI CCNNNNNNNGG 2 cut(s) 115, 428
AgsI TTSAA 1 cut(s) 389
AjnI CCWGG 2 cut(s) 125, 134
AluBI AGCT 2 cut(s) 175, 324
AluI AGCT 2 cut(s) 175, 324
Alw21I GWGCWC 1 cut(s) 87
AlwI GGATC 2 cut(s) 188, 201
AlwNI CAGNNNCTG 1 cut(s) 247
Ama87I CYCGRG 1 cut(s) 80
Aor51HI AGCGCT 1 cut(s) 27
AoxI GGCC 1 cut(s) 128
ApeKI GCWGC 1 cut(s) 247
AspA2I CCTAGG 1 cut(s) 31
AspLEI GCGC 1 cut(s) 28
AspS9I GGNCC 1 cut(s) 129
AsuHPI GGTGA 3 cut(s) 6, 55, 353
AvaI CYCGRG 1 cut(s) 80
AvrII CCTAGG 1 cut(s) 31
BamHI GGATCC 1 cut(s) 193
BanI GGYRCC 1 cut(s) 329
BanII GRGCYC 1 cut(s) 209
BauI CACGAG 1 cut(s) 95
BbsI GAAGAC 1 cut(s) 45
Bbv12I GWGCWC 1 cut(s) 87
BbvI GCAGC 1 cut(s) 234
BccI CCATC 2 cut(s) 133, 439
BceAI ACGGC 1 cut(s) 320
BciT130I CCWGG 2 cut(s) 127, 136
BclI TGATCA 2 cut(s) 157, 268
BfaI CTAG 1 cut(s) 32
BfoI RGCGCY 1 cut(s) 29
BisI GCNGC 2 cut(s) 248, 333
BlnI CCTAGG 1 cut(s) 31
BlsI GCNGC 2 cut(s) 249, 334
Bme1390I CCNGG 2 cut(s) 127, 136
BmeT110I CYCGRG 1 cut(s) 80
BmgT120I GGNCC 1 cut(s) 129
BmiI GGNNCC 2 cut(s) 195, 331
BmrFI CCNGG 2 cut(s) 127, 136
BmrI ACTGGG 2 cut(s) 124, 203
BmsI GCATC 1 cut(s) 81
BmuI ACTGGG 2 cut(s) 124, 203
BpiI GAAGAC 1 cut(s) 45
BplI GAGNNNNNCTC 1 cut(s) 28
BpmI CTGGAG 1 cut(s) 118
BsaHI GRCGYC 1 cut(s) 48
BsaJI CCNNGG 1 cut(s) 31
BsaWI WCCGGW 1 cut(s) 196
BsaXI ACNNNNNCTCC 2 cut(s) 12, 42
Bsc4I CCNNNNNNNGG 2 cut(s) 115, 428
Bse118I RCCGGY 1 cut(s) 421
Bse1I ACTGG 2 cut(s) 119, 209
BseBI CCWGG 2 cut(s) 127, 136
BseDI CCNNGG 1 cut(s) 31
BseGI GGATG 1 cut(s) 144
BseLI CCNNNNNNNGG 2 cut(s) 115, 428
BseNI ACTGG 2 cut(s) 119, 209
BseRI GAGGAG 2 cut(s) 330, 414
BseXI GCAGC 1 cut(s) 234
BshFI GGCC 1 cut(s) 130
BshNI GGYRCC 1 cut(s) 329
BsiHKAI GWGCWC 1 cut(s) 87
BsiHKCI CYCGRG 1 cut(s) 80
BsiSI CCGG 2 cut(s) 197, 422
BslI CCNNNNNNNGG 2 cut(s) 115, 428
BsnI GGCC 1 cut(s) 130
BsoBI CYCGRG 1 cut(s) 80
Bsp1286I GDGCHC 2 cut(s) 87, 209
Bsp143I GATC 5 cut(s) 54, 157, 193, 259, 268
BspACI CCGC 1 cut(s) 333
BspANI GGCC 1 cut(s) 130
BspLI GGNNCC 2 cut(s) 195, 331
BspPI GGATC 2 cut(s) 188, 201
BspT107I GGYRCC 1 cut(s) 329
BsrFI RCCGGY 1 cut(s) 421
BsrI ACTGG 2 cut(s) 119, 209
BssAI RCCGGY 1 cut(s) 421
BssECI CCNNGG 1 cut(s) 31
BssMI GATC 5 cut(s) 54, 157, 193, 259, 268
BssNI GRCGYC 1 cut(s) 48
BssSI CACGAG 1 cut(s) 95
BssT1I CCWWGG 1 cut(s) 31
Bst2BI CACGAG 1 cut(s) 95
Bst2UI CCWGG 2 cut(s) 127, 136
Bst4CI ACNGT 1 cut(s) 329
Bst6I CTCTTC 1 cut(s) 156
BstACI GRCGYC 1 cut(s) 48
BstAPI GCANNNNNTGC 3 cut(s) 91, 238, 247
BstF5I GGATG 1 cut(s) 144
BstH2I RGCGCY 1 cut(s) 29
BstHHI GCGC 1 cut(s) 28
BstKTI GATC 5 cut(s) 57, 160, 196, 262, 271
BstMBI GATC 5 cut(s) 54, 157, 193, 259, 268
BstMWI GCNNNNNNNGC 3 cut(s) 91, 238, 247
BstNI CCWGG 2 cut(s) 127, 136
BstNSI RCATGY 1 cut(s) 355
BstSCI CCNGG 2 cut(s) 125, 134
BstV1I GCAGC 1 cut(s) 234
BstV2I GAAGAC 1 cut(s) 45
BstX2I RGATCY 1 cut(s) 193
BstYI RGATCY 1 cut(s) 193
BsuRI GGCC 1 cut(s) 130
BtsCI GGATG 1 cut(s) 144
BtsIMutI CAGTG 1 cut(s) 216
CaiI CAGNNNCTG 1 cut(s) 247
CfoI GCGC 1 cut(s) 28
Cfr10I RCCGGY 1 cut(s) 421
Cfr13I GGNCC 1 cut(s) 129
CseI GACGC 1 cut(s) 56
Csp6I GTAC 1 cut(s) 100
CviAII CATG 2 cut(s) 88, 352
CviJI RGCY 8 cut(s) 108, 119, 130, 143, 175, 207, 247, 324
CviKI_1 RGCY 8 cut(s) 108, 119, 130, 143, 175, 207, 247, 324
CviQI GTAC 1 cut(s) 100
DpnI GATC 5 cut(s) 56, 159, 195, 261, 270
DpnII GATC 5 cut(s) 54, 157, 193, 259, 268
Eam1104I CTCTTC 1 cut(s) 156
EarI CTCTTC 1 cut(s) 156
Eco130I CCWWGG 1 cut(s) 31
Eco24I GRGCYC 1 cut(s) 209
Eco47III AGCGCT 1 cut(s) 27
Eco57I CTGAAG 1 cut(s) 161
Eco88I CYCGRG 1 cut(s) 80
EcoO109I RGGNCCY 1 cut(s) 129
EcoRII CCWGG 2 cut(s) 125, 134
EcoT14I CCWWGG 1 cut(s) 31
EcoT38I GRGCYC 1 cut(s) 209
ErhI CCWWGG 1 cut(s) 31
FaeI CATG 2 cut(s) 91, 355
FaiI YATR 3 cut(s) 89, 353, 369
FatI CATG 2 cut(s) 87, 351
FbaI TGATCA 2 cut(s) 157, 268
Fnu4HI GCNGC 2 cut(s) 248, 333
FokI GGATG 1 cut(s) 151
FriOI GRGCYC 1 cut(s) 209
Fsp4HI GCNGC 2 cut(s) 248, 333
FspBI CTAG 1 cut(s) 32
GlaI GCGC 1 cut(s) 27
GluI GCNGC 2 cut(s) 248, 333
GsuI CTGGAG 1 cut(s) 118
HaeII RGCGCY 1 cut(s) 29
HaeIII GGCC 1 cut(s) 130
HapII CCGG 2 cut(s) 197, 422
HgaI GACGC 1 cut(s) 56
HhaI GCGC 1 cut(s) 28
Hin1I GRCGYC 1 cut(s) 48
Hin1II CATG 2 cut(s) 91, 355
Hin6I GCGC 1 cut(s) 26
HinP1I GCGC 1 cut(s) 26
HpaII CCGG 2 cut(s) 197, 422
HphI GGTGA 3 cut(s) 6, 55, 353
Hpy188I TCNGA 2 cut(s) 193, 308
Hpy188III TCNNGA 2 cut(s) 155, 220
Hpy99I CGWCG 3 cut(s) 50, 74, 341
HpyAV CCTTC 1 cut(s) 352
HpyCH4III ACNGT 1 cut(s) 329
HpyCH4V TGCA 5 cut(s) 94, 232, 241, 250, 355
HpyF10VI GCNNNNNNNGC 3 cut(s) 91, 238, 247
Hsp92I GRCGYC 1 cut(s) 48
Hsp92II CATG 2 cut(s) 91, 355
HspAI GCGC 1 cut(s) 26
Ksp22I TGATCA 2 cut(s) 157, 268
Kzo9I GATC 5 cut(s) 54, 157, 193, 259, 268
LmnI GCTCC 2 cut(s) 33, 308
Lsp1109I GCAGC 1 cut(s) 234
LweI GCATC 1 cut(s) 81
MaeI CTAG 1 cut(s) 32
MaeIII GTNAC 2 cut(s) 61, 376
MalI GATC 5 cut(s) 56, 159, 195, 261, 270
MboI GATC 5 cut(s) 54, 157, 193, 259, 268
MboII GAAGA 3 cut(s) 50, 143, 294
MfeI CAATTG 1 cut(s) 227
MflI RGATCY 1 cut(s) 193
MhlI GDGCHC 2 cut(s) 87, 209
MluCI AATT 3 cut(s) 227, 251, 391
MmeI TCCRAC 1 cut(s) 171
MspI CCGG 2 cut(s) 197, 422
MspR9I CCNGG 2 cut(s) 127, 136
MunI CAATTG 1 cut(s) 227
MvaI CCWGG 2 cut(s) 127, 136
MwoI GCNNNNNNNGC 3 cut(s) 91, 238, 247
NdeII GATC 5 cut(s) 54, 157, 193, 259, 268
NlaIII CATG 2 cut(s) 91, 355
NlaIV GGNNCC 2 cut(s) 195, 331
NmuCI GTSAC 1 cut(s) 61
NspI RCATGY 1 cut(s) 355
PaeR7I CTCGAG 1 cut(s) 80
PcsI WCGNNNNNNNCGW 2 cut(s) 78, 333
PfoI TCCNGGA 1 cut(s) 134
PkrI GCNGC 2 cut(s) 249, 334
Psp6I CCWGG 2 cut(s) 125, 134
PspGI CCWGG 2 cut(s) 125, 134
PspN4I GGNNCC 2 cut(s) 195, 331
PspPI GGNCC 1 cut(s) 129
PspXI VCTCGAGB 1 cut(s) 80
PstNI CAGNNNCTG 1 cut(s) 247
PsuI RGATCY 1 cut(s) 193
RsaI GTAC 1 cut(s) 101
RsaNI GTAC 1 cut(s) 100
SatI GCNGC 2 cut(s) 248, 333
Sau3AI GATC 5 cut(s) 54, 157, 193, 259, 268
Sau96I GGNCC 1 cut(s) 129
ScrFI CCNGG 2 cut(s) 127, 136
SduI GDGCHC 2 cut(s) 87, 209
SetI ASST 9 cut(s) 19, 45, 81, 177, 183, 303, 326, 363, 406
SfaNI GCATC 1 cut(s) 81
Sfr274I CTCGAG 1 cut(s) 80
SgrAI CRCCGGYG 1 cut(s) 421
SlaI CTCGAG 1 cut(s) 80
SmlI CTYRAG 1 cut(s) 80
SmoI CTYRAG 1 cut(s) 80
Sse9I AATT 3 cut(s) 227, 251, 391
SsiI CCGC 1 cut(s) 333
SspMI CTAG 1 cut(s) 32
StyD4I CCNGG 2 cut(s) 125, 134
StyI CCWWGG 1 cut(s) 31
TaaI ACNGT 1 cut(s) 329
TaqI TCGA 3 cut(s) 45, 81, 280
TasI AATT 3 cut(s) 227, 251, 391
TauI GCSGC 1 cut(s) 335
TscAI CASTG 1 cut(s) 216
TseFI GTSAC 1 cut(s) 61
TseI GCWGC 1 cut(s) 247
Tsp45I GTSAC 1 cut(s) 61
TspGWI ACGGA 1 cut(s) 49
TspRI CASTG 1 cut(s) 216
XceI RCATGY 1 cut(s) 355
XhoI CTCGAG 1 cut(s) 80
XmaJI CCTAGG 1 cut(s) 31
XspI CTAG 1 cut(s) 32
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.