Rh5CG407400

PB1 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0018125
Physical Location & Seq
Reverse (-)
54695012 .. 54695188
177 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG407400.1

Sequence Viewer

Length: 177 bp
ATGTCATTCAAGTACCAGCTCCTATGGGAAGACCTCGACACCTTGATCTCCGTCACCAACGATGACAACCTCGAGCACATGATGCACGAGTACGATAGCCTCTATTGGGCTTTCACCAGGCCCACCAGGATGAATGTTTCTCTTCCCGATCAATCACAGCAACAGCTTCAGGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PB1

Protein Analysis

58

Amino Acids

7.02

Weight (kDa)

4.3

Isoelectric Point (pI)

53.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PB1 PF00564 2 - 39 5.6e-08 PB1 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcuI CTGAAG 1 cut(s) 152
AfaI GTAC 2 cut(s) 14, 92
AfiI CCNNNNNNNGG 1 cut(s) 106
AgsI TTSAA 1 cut(s) 10
AjnI CCWGG 2 cut(s) 116, 125
AluBI AGCT 2 cut(s) 19, 166
AluI AGCT 2 cut(s) 19, 166
Alw21I GWGCWC 1 cut(s) 78
Ama87I CYCGRG 1 cut(s) 71
AoxI GGCC 1 cut(s) 119
AspS9I GGNCC 1 cut(s) 120
AsuHPI GGTGA 2 cut(s) 46, 106
AvaI CYCGRG 1 cut(s) 71
BauI CACGAG 1 cut(s) 86
BbsI GAAGAC 1 cut(s) 36
Bbv12I GWGCWC 1 cut(s) 78
BciT130I CCWGG 2 cut(s) 118, 127
Bme1390I CCNGG 2 cut(s) 118, 127
BmeT110I CYCGRG 1 cut(s) 71
BmgT120I GGNCC 1 cut(s) 120
BmrFI CCNGG 2 cut(s) 118, 127
BmsI GCATC 1 cut(s) 72
BpiI GAAGAC 1 cut(s) 36
Bsc4I CCNNNNNNNGG 1 cut(s) 106
BseBI CCWGG 2 cut(s) 118, 127
BseGI GGATG 1 cut(s) 135
BseLI CCNNNNNNNGG 1 cut(s) 106
BshFI GGCC 1 cut(s) 121
BsiHKAI GWGCWC 1 cut(s) 78
BsiHKCI CYCGRG 1 cut(s) 71
BslI CCNNNNNNNGG 1 cut(s) 106
BsnI GGCC 1 cut(s) 121
BsoBI CYCGRG 1 cut(s) 71
Bsp1286I GDGCHC 1 cut(s) 78
Bsp143I GATC 2 cut(s) 45, 148
BspANI GGCC 1 cut(s) 121
BssMI GATC 2 cut(s) 45, 148
BssSI CACGAG 1 cut(s) 86
Bst2BI CACGAG 1 cut(s) 86
Bst2UI CCWGG 2 cut(s) 118, 127
Bst6I CTCTTC 1 cut(s) 147
BstAPI GCANNNNNTGC 1 cut(s) 82
BstF5I GGATG 1 cut(s) 135
BstKTI GATC 2 cut(s) 48, 151
BstMBI GATC 2 cut(s) 45, 148
BstMWI GCNNNNNNNGC 1 cut(s) 82
BstNI CCWGG 2 cut(s) 118, 127
BstSCI CCNGG 2 cut(s) 116, 125
BstV2I GAAGAC 1 cut(s) 36
BsuRI GGCC 1 cut(s) 121
BtsCI GGATG 1 cut(s) 135
Cfr13I GGNCC 1 cut(s) 120
Csp6I GTAC 2 cut(s) 13, 91
CviAII CATG 1 cut(s) 79
CviJI RGCY 5 cut(s) 19, 99, 110, 121, 166
CviKI_1 RGCY 5 cut(s) 19, 99, 110, 121, 166
CviQI GTAC 2 cut(s) 13, 91
DpnI GATC 2 cut(s) 47, 150
DpnII GATC 2 cut(s) 45, 148
Eam1104I CTCTTC 1 cut(s) 147
EarI CTCTTC 1 cut(s) 147
Eco57I CTGAAG 1 cut(s) 152
Eco88I CYCGRG 1 cut(s) 71
EcoRII CCWGG 2 cut(s) 116, 125
FaeI CATG 1 cut(s) 82
FaiI YATR 2 cut(s) 25, 80
FatI CATG 1 cut(s) 78
FokI GGATG 1 cut(s) 142
HaeIII GGCC 1 cut(s) 121
Hin1II CATG 1 cut(s) 82
HphI GGTGA 2 cut(s) 46, 106
Hpy188III TCNNGA 1 cut(s) 146
HpyCH4V TGCA 1 cut(s) 85
HpyF10VI GCNNNNNNNGC 1 cut(s) 82
Hsp92II CATG 1 cut(s) 82
Kzo9I GATC 2 cut(s) 45, 148
LmnI GCTCC 1 cut(s) 24
LpnPI CCDG 6 cut(s) 29, 103, 112, 130, 139, 155
LweI GCATC 1 cut(s) 72
MaeIII GTNAC 1 cut(s) 52
MalI GATC 2 cut(s) 47, 150
MboI GATC 2 cut(s) 45, 148
MboII GAAGA 2 cut(s) 41, 134
MhlI GDGCHC 1 cut(s) 78
MnlI CCTC 3 cut(s) 44, 80, 110
MslI CAYNNNNRTG 1 cut(s) 128
MspR9I CCNGG 2 cut(s) 118, 127
MvaI CCWGG 2 cut(s) 118, 127
MwoI GCNNNNNNNGC 1 cut(s) 82
NdeII GATC 2 cut(s) 45, 148
NlaIII CATG 1 cut(s) 82
NmuCI GTSAC 1 cut(s) 52
PaeR7I CTCGAG 1 cut(s) 71
Psp6I CCWGG 2 cut(s) 116, 125
PspGI CCWGG 2 cut(s) 116, 125
PspPI GGNCC 1 cut(s) 120
PspXI VCTCGAGB 1 cut(s) 71
RsaI GTAC 2 cut(s) 14, 92
RsaNI GTAC 2 cut(s) 13, 91
RseI CAYNNNNRTG 1 cut(s) 128
Sau3AI GATC 2 cut(s) 45, 148
Sau96I GGNCC 1 cut(s) 120
ScrFI CCNGG 2 cut(s) 118, 127
SduI GDGCHC 1 cut(s) 78
SetI ASST 6 cut(s) 21, 36, 44, 72, 168, 174
SfaNI GCATC 1 cut(s) 72
Sfr274I CTCGAG 1 cut(s) 71
SlaI CTCGAG 1 cut(s) 71
SmiMI CAYNNNNRTG 1 cut(s) 128
SmlI CTYRAG 1 cut(s) 71
SmoI CTYRAG 1 cut(s) 71
StyD4I CCNGG 2 cut(s) 116, 125
TaqI TCGA 2 cut(s) 36, 72
TseFI GTSAC 1 cut(s) 52
Tsp45I GTSAC 1 cut(s) 52
TspDTI ATGAA 1 cut(s) 146
TspGWI ACGGA 1 cut(s) 40
XhoI CTCGAG 1 cut(s) 71
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.