Rh5AG373200

PB1 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0018125
Physical Location & Seq
Reverse (-)
63462777 .. 63463308
532 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG373200.1

Sequence Viewer

Length: 336 bp
ATGTCATTCAAGTACCAGCTCCTAGGGGAAGACCTCGACGCCTTAATCTCAGTCACCAACGACGACAACCTCGAGCACATGATGCACGAGTACGATAGCCGCTACTGGGCTTTCACTAGGCCCACTAGGATGACTGTTTCTCTTCCCGATCAATCACAACAACAACTTCAGGAGGAGGAAGCTATGGTGCCGCCGTCGGTGATTACTACATGCAGAAGGTGTCGGAAAGCCTCATCCGATGACGATGCCTCAGTAGATTTTGGCTCCGACAGGTTACTGGCCGGAAAAGCATGTAGCGAGCGTCAGATTCTCTGGGATAGTGACGGGAGCACCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PB1

Protein Analysis

111

Amino Acids

12.64

Weight (kDa)

4.47

Isoelectric Point (pI)

62.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PB1 PF00564 2 - 36 9.8e-08 PB1 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 187
AciI CCGC 2 cut(s) 100, 191
AcoI YGGCCR 1 cut(s) 279
AcuI CTGAAG 1 cut(s) 152
AcyI GRCGYC 1 cut(s) 39
AfaI GTAC 2 cut(s) 14, 92
AfiI CCNNNNNNNGG 1 cut(s) 106
AgsI TTSAA 1 cut(s) 10
AluBI AGCT 2 cut(s) 19, 182
AluI AGCT 2 cut(s) 19, 182
Alw21I GWGCWC 2 cut(s) 78, 332
Ama87I CYCGRG 1 cut(s) 71
AoxI GGCC 2 cut(s) 119, 279
AspA2I CCTAGG 1 cut(s) 22
AspS9I GGNCC 1 cut(s) 120
AsuHPI GGTGA 2 cut(s) 46, 211
AvaI CYCGRG 1 cut(s) 71
AvrII CCTAGG 1 cut(s) 22
BanI GGYRCC 1 cut(s) 187
BauI CACGAG 1 cut(s) 86
BbsI GAAGAC 1 cut(s) 36
Bbv12I GWGCWC 2 cut(s) 78, 332
BceAI ACGGC 1 cut(s) 178
BcgI CGANNNNNNTGC 2 cut(s) 227, 261
BfaI CTAG 3 cut(s) 23, 117, 126
BisI GCNGC 2 cut(s) 100, 191
BlnI CCTAGG 1 cut(s) 22
BlsI GCNGC 2 cut(s) 101, 192
BmeT110I CYCGRG 1 cut(s) 71
BmgT120I GGNCC 1 cut(s) 120
BmiI GGNNCC 2 cut(s) 189, 265
BmrI ACTGGG 1 cut(s) 115
BmsI GCATC 2 cut(s) 72, 235
BmuI ACTGGG 1 cut(s) 115
BpiI GAAGAC 1 cut(s) 36
BsaHI GRCGYC 1 cut(s) 39
BsaJI CCNNGG 1 cut(s) 22
Bsc4I CCNNNNNNNGG 1 cut(s) 106
Bse1I ACTGG 2 cut(s) 110, 282
BseDI CCNNGG 1 cut(s) 22
BseGI GGATG 2 cut(s) 135, 233
BseLI CCNNNNNNNGG 1 cut(s) 106
BseMII CTCAG 2 cut(s) 63, 264
BseNI ACTGG 2 cut(s) 110, 282
BseRI GAGGAG 1 cut(s) 188
BshFI GGCC 2 cut(s) 121, 281
BshNI GGYRCC 1 cut(s) 187
BsiHKAI GWGCWC 2 cut(s) 78, 332
BsiHKCI CYCGRG 1 cut(s) 71
BsiSI CCGG 1 cut(s) 282
BslI CCNNNNNNNGG 1 cut(s) 106
BsnI GGCC 2 cut(s) 121, 281
BsoBI CYCGRG 1 cut(s) 71
Bsp1286I GDGCHC 2 cut(s) 78, 332
Bsp143I GATC 1 cut(s) 148
BspACI CCGC 2 cut(s) 100, 191
BspANI GGCC 2 cut(s) 121, 281
BspCNI CTCAG 2 cut(s) 62, 263
BspLI GGNNCC 2 cut(s) 189, 265
BspT107I GGYRCC 1 cut(s) 187
BsrI ACTGG 2 cut(s) 110, 282
BssECI CCNNGG 1 cut(s) 22
BssMI GATC 1 cut(s) 148
BssNI GRCGYC 1 cut(s) 39
BssSI CACGAG 1 cut(s) 86
BssT1I CCWWGG 1 cut(s) 22
Bst2BI CACGAG 1 cut(s) 86
Bst4CI ACNGT 1 cut(s) 136
Bst6I CTCTTC 1 cut(s) 147
BstACI GRCGYC 1 cut(s) 39
BstAPI GCANNNNNTGC 1 cut(s) 82
BstC8I GCNNGC 1 cut(s) 299
BstDEI CTNAG 2 cut(s) 49, 250
BstF5I GGATG 2 cut(s) 135, 233
BstKTI GATC 1 cut(s) 151
BstMBI GATC 1 cut(s) 148
BstMWI GCNNNNNNNGC 2 cut(s) 82, 287
BstNSI RCATGY 2 cut(s) 213, 294
BstV2I GAAGAC 1 cut(s) 36
BsuRI GGCC 2 cut(s) 121, 281
BtsCI GGATG 2 cut(s) 135, 233
Cac8I GCNNGC 1 cut(s) 299
Cfr13I GGNCC 1 cut(s) 120
CseI GACGC 2 cut(s) 47, 290
Csp6I GTAC 2 cut(s) 13, 91
CviAII CATG 3 cut(s) 79, 210, 291
CviJI RGCY 8 cut(s) 19, 99, 110, 121, 182, 230, 264, 281
CviKI_1 RGCY 8 cut(s) 19, 99, 110, 121, 182, 230, 264, 281
CviQI GTAC 2 cut(s) 13, 91
DdeI CTNAG 2 cut(s) 49, 250
DpnI GATC 1 cut(s) 150
DpnII GATC 1 cut(s) 148
EaeI YGGCCR 1 cut(s) 279
Eam1104I CTCTTC 1 cut(s) 147
EarI CTCTTC 1 cut(s) 147
Eco130I CCWWGG 1 cut(s) 22
Eco57I CTGAAG 1 cut(s) 152
Eco88I CYCGRG 1 cut(s) 71
EcoT14I CCWWGG 1 cut(s) 22
ErhI CCWWGG 1 cut(s) 22
FaeI CATG 3 cut(s) 82, 213, 294
FaiI YATR 4 cut(s) 80, 185, 211, 292
FatI CATG 3 cut(s) 78, 209, 290
Fnu4HI GCNGC 2 cut(s) 100, 191
FokI GGATG 2 cut(s) 142, 220
Fsp4HI GCNGC 2 cut(s) 100, 191
FspBI CTAG 3 cut(s) 23, 117, 126
GluI GCNGC 2 cut(s) 100, 191
HaeIII GGCC 2 cut(s) 121, 281
HapII CCGG 1 cut(s) 282
HgaI GACGC 2 cut(s) 47, 290
Hin1I GRCGYC 1 cut(s) 39
Hin1II CATG 3 cut(s) 82, 213, 294
HinfI GANTC 1 cut(s) 307
HpaII CCGG 1 cut(s) 282
HphI GGTGA 2 cut(s) 46, 211
Hpy188I TCNGA 4 cut(s) 225, 238, 268, 306
Hpy188III TCNNGA 2 cut(s) 146, 170
Hpy99I CGWCG 3 cut(s) 41, 65, 199
HpyAV CCTTC 1 cut(s) 210
HpyCH4III ACNGT 1 cut(s) 136
HpyCH4V TGCA 2 cut(s) 85, 213
HpyF10VI GCNNNNNNNGC 2 cut(s) 82, 287
HpyF3I CTNAG 2 cut(s) 49, 250
Hsp92I GRCGYC 1 cut(s) 39
Hsp92II CATG 3 cut(s) 82, 213, 294
Kzo9I GATC 1 cut(s) 148
LmnI GCTCC 3 cut(s) 24, 269, 327
LpnPI CCDG 7 cut(s) 29, 91, 155, 256, 263, 295, 298
LweI GCATC 2 cut(s) 72, 235
MaeI CTAG 3 cut(s) 23, 117, 126
MaeIII GTNAC 3 cut(s) 52, 273, 320
MalI GATC 1 cut(s) 150
MboI GATC 1 cut(s) 148
MboII GAAGA 2 cut(s) 41, 134
MhlI GDGCHC 2 cut(s) 78, 332
MmeI TCCRAC 2 cut(s) 203, 291
MnlI CCTC 6 cut(s) 44, 80, 166, 169, 241, 259
MseI TTAA 1 cut(s) 44
MslI CAYNNNNRTG 1 cut(s) 128
MspI CCGG 1 cut(s) 282
MwoI GCNNNNNNNGC 2 cut(s) 82, 287
NdeII GATC 1 cut(s) 148
NlaIII CATG 3 cut(s) 82, 213, 294
NlaIV GGNNCC 2 cut(s) 189, 265
NmuCI GTSAC 2 cut(s) 52, 320
NspI RCATGY 2 cut(s) 213, 294
PaeR7I CTCGAG 1 cut(s) 71
PcsI WCGNNNNNNNCGW 1 cut(s) 69
PfeI GAWTC 1 cut(s) 307
PkrI GCNGC 2 cut(s) 101, 192
PspN4I GGNNCC 2 cut(s) 189, 265
PspPI GGNCC 1 cut(s) 120
PspXI VCTCGAGB 1 cut(s) 71
RsaI GTAC 2 cut(s) 14, 92
RsaNI GTAC 2 cut(s) 13, 91
RseI CAYNNNNRTG 1 cut(s) 128
SaqAI TTAA 1 cut(s) 44
SatI GCNGC 2 cut(s) 100, 191
Sau3AI GATC 1 cut(s) 148
Sau96I GGNCC 1 cut(s) 120
SduI GDGCHC 2 cut(s) 78, 332
SetI ASST 7 cut(s) 21, 36, 72, 184, 221, 275, 335
SfaNI GCATC 2 cut(s) 72, 235
Sfr274I CTCGAG 1 cut(s) 71
SlaI CTCGAG 1 cut(s) 71
SmiMI CAYNNNNRTG 1 cut(s) 128
SmlI CTYRAG 1 cut(s) 71
SmoI CTYRAG 1 cut(s) 71
SsiI CCGC 2 cut(s) 100, 191
SspMI CTAG 3 cut(s) 23, 117, 126
StyI CCWWGG 1 cut(s) 22
TaaI ACNGT 1 cut(s) 136
TaqI TCGA 2 cut(s) 36, 72
TauI GCSGC 2 cut(s) 102, 193
TfiI GAWTC 1 cut(s) 307
Tru1I TTAA 1 cut(s) 44
Tru9I TTAA 1 cut(s) 44
TseFI GTSAC 2 cut(s) 52, 320
Tsp45I GTSAC 2 cut(s) 52, 320
XceI RCATGY 2 cut(s) 213, 294
XhoI CTCGAG 1 cut(s) 71
XmaJI CCTAGG 1 cut(s) 22
XspI CTAG 3 cut(s) 23, 117, 126
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.