Rmu_sc0042355.1_g000001

PB1 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0042355.1
OG0018125
Physical Location & Seq
Reverse (-)
1170 .. 1411
242 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0042355.1_g000001.1.cds

Sequence Viewer

Length: 242 bp
gcaccatcaagttctccgccctcatcggcaggctctccgccctctctaacaacgacgtgtcgttcaagtaccagctccccggggaagaccttgacgccttgatctcggtcaccaacgacgacgacctcaagcacatgatgcataggcccgccaagatgaggctatttctcttctcggtcaatcacagtgacagtttcggtttgacaggtcgtccgatagggatcacttcgttgaggctttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PB1

Protein Analysis

79

Amino Acids

8.81

Weight (kDa)

8.13

Isoelectric Point (pI)

26.86

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 17, 38, 149
AclWI GGATC 1 cut(s) 229
AcyI GRCGYC 1 cut(s) 95
AfaI GTAC 1 cut(s) 70
AfiI CCNNNNNNNGG 1 cut(s) 158
AflIII ACRYGT 1 cut(s) 56
AgsI TTSAA 1 cut(s) 66
AhdI GACNNNNNGTC 1 cut(s) 209
AjiI CACGTC 1 cut(s) 57
AluBI AGCT 1 cut(s) 75
AluI AGCT 1 cut(s) 75
AlwI GGATC 1 cut(s) 229
Ama87I CYCGRG 1 cut(s) 79
AoxI GGCC 1 cut(s) 145
AspS9I GGNCC 1 cut(s) 146
AsuC2I CCSGG 2 cut(s) 80, 81
AsuHPI GGTGA 1 cut(s) 102
AvaI CYCGRG 1 cut(s) 79
BbsI GAAGAC 1 cut(s) 92
BccI CCATC 1 cut(s) 13
BcnI CCSGG 2 cut(s) 80, 81
Bme1390I CCNGG 2 cut(s) 80, 81
BmeRI GACNNNNNGTC 1 cut(s) 209
BmeT110I CYCGRG 1 cut(s) 79
BmgBI CACGTC 1 cut(s) 57
BmgT120I GGNCC 1 cut(s) 146
BmrFI CCNGG 2 cut(s) 80, 81
BmsI GCATC 1 cut(s) 128
BpiI GAAGAC 1 cut(s) 92
BpuEI CTTGAG 1 cut(s) 112
BpuMI CCSGG 2 cut(s) 80, 81
BsaBI GATNNNNATC 1 cut(s) 220
BsaHI GRCGYC 1 cut(s) 95
BsaJI CCNNGG 3 cut(s) 78, 79, 80
Bsc4I CCNNNNNNNGG 1 cut(s) 158
Bse8I GATNNNNATC 1 cut(s) 220
BseDI CCNNGG 3 cut(s) 78, 79, 80
BseJI GATNNNNATC 1 cut(s) 220
BseLI CCNNNNNNNGG 1 cut(s) 158
BshFI GGCC 1 cut(s) 147
BsiHKCI CYCGRG 1 cut(s) 79
BsiSI CCGG 1 cut(s) 80
BslI CCNNNNNNNGG 1 cut(s) 158
BsnI GGCC 1 cut(s) 147
BsoBI CYCGRG 1 cut(s) 79
Bsp143I GATC 2 cut(s) 101, 221
BspACI CCGC 3 cut(s) 17, 38, 149
BspANI GGCC 1 cut(s) 147
BspPI GGATC 1 cut(s) 229
BssECI CCNNGG 3 cut(s) 78, 79, 80
BssMI GATC 2 cut(s) 101, 221
BssNI GRCGYC 1 cut(s) 95
Bst4CI ACNGT 2 cut(s) 187, 193
Bst6I CTCTTC 1 cut(s) 175
BstACI GRCGYC 1 cut(s) 95
BstAPI GCANNNNNTGC 1 cut(s) 138
BstC8I GCNNGC 2 cut(s) 31, 149
BstEII GGTNACC 1 cut(s) 108
BstKTI GATC 2 cut(s) 104, 224
BstMBI GATC 2 cut(s) 101, 221
BstMWI GCNNNNNNNGC 1 cut(s) 138
BstPI GGTNACC 1 cut(s) 108
BstSCI CCNGG 2 cut(s) 78, 79
BstV2I GAAGAC 1 cut(s) 92
BsuRI GGCC 1 cut(s) 147
BtrI CACGTC 1 cut(s) 57
BtsIMutI CAGTG 1 cut(s) 192
Cac8I GCNNGC 2 cut(s) 31, 149
Cfr13I GGNCC 1 cut(s) 146
Cfr9I CCCGGG 1 cut(s) 79
CseI GACGC 1 cut(s) 103
Csp6I GTAC 1 cut(s) 69
CviAII CATG 1 cut(s) 135
CviJI RGCY 5 cut(s) 33, 75, 147, 162, 237
CviKI_1 RGCY 5 cut(s) 33, 75, 147, 162, 237
CviQI GTAC 1 cut(s) 69
DpnI GATC 2 cut(s) 103, 223
DpnII GATC 2 cut(s) 101, 221
DriI GACNNNNNGTC 1 cut(s) 209
Eam1104I CTCTTC 1 cut(s) 175
Eam1105I GACNNNNNGTC 1 cut(s) 209
EarI CTCTTC 1 cut(s) 175
EciI GGCGGA 2 cut(s) 6, 27
Eco88I CYCGRG 1 cut(s) 79
Eco91I GGTNACC 1 cut(s) 108
EcoO65I GGTNACC 1 cut(s) 108
EcoT22I ATGCAT 1 cut(s) 143
FaeI CATG 1 cut(s) 138
FaiI YATR 2 cut(s) 136, 143
FatI CATG 1 cut(s) 134
FauI CCCGC 1 cut(s) 156
HaeIII GGCC 1 cut(s) 147
HapII CCGG 1 cut(s) 80
HgaI GACGC 1 cut(s) 103
Hin1I GRCGYC 1 cut(s) 95
Hin1II CATG 1 cut(s) 138
HpaII CCGG 1 cut(s) 80
HphI GGTGA 1 cut(s) 102
Hpy188I TCNGA 1 cut(s) 215
Hpy99I CGWCG 3 cut(s) 58, 121, 124
HpyCH4III ACNGT 2 cut(s) 187, 193
HpyCH4IV ACGT 1 cut(s) 56
HpyCH4V TGCA 1 cut(s) 141
HpyF10VI GCNNNNNNNGC 1 cut(s) 138
HpySE526I ACGT 1 cut(s) 56
Hsp92I GRCGYC 1 cut(s) 95
Hsp92II CATG 1 cut(s) 138
Kzo9I GATC 2 cut(s) 101, 221
LmnI GCTCC 1 cut(s) 80
LpnPI CCDG 4 cut(s) 15, 85, 93, 191
LweI GCATC 1 cut(s) 128
MaeII ACGT 1 cut(s) 56
MaeIII GTNAC 2 cut(s) 108, 187
MalI GATC 2 cut(s) 103, 223
MboI GATC 2 cut(s) 101, 221
MboII GAAGA 2 cut(s) 97, 162
MnlI CCTC 5 cut(s) 31, 52, 136, 152, 227
Mph1103I ATGCAT 1 cut(s) 143
MspI CCGG 1 cut(s) 80
MspR9I CCNGG 2 cut(s) 80, 81
MwoI GCNNNNNNNGC 1 cut(s) 138
NciI CCSGG 2 cut(s) 80, 81
NdeII GATC 2 cut(s) 101, 221
NlaIII CATG 1 cut(s) 138
NmuCI GTSAC 2 cut(s) 108, 187
NsiI ATGCAT 1 cut(s) 143
PspEI GGTNACC 1 cut(s) 108
PspPI GGNCC 1 cut(s) 146
RsaI GTAC 1 cut(s) 70
RsaNI GTAC 1 cut(s) 69
Sau3AI GATC 2 cut(s) 101, 221
Sau96I GGNCC 1 cut(s) 146
ScrFI CCNGG 2 cut(s) 80, 81
SetI ASST 5 cut(s) 59, 77, 92, 128, 210
SfaNI GCATC 1 cut(s) 128
SmaI CCCGGG 1 cut(s) 81
SmlI CTYRAG 1 cut(s) 127
SmoI CTYRAG 1 cut(s) 127
SsiI CCGC 3 cut(s) 17, 38, 149
StyD4I CCNGG 2 cut(s) 78, 79
TaaI ACNGT 2 cut(s) 187, 193
TaiI ACGT 1 cut(s) 59
TaqII GACCGA 2 cut(s) 96, 165
TscAI CASTG 1 cut(s) 192
TseFI GTSAC 2 cut(s) 108, 187
Tsp45I GTSAC 2 cut(s) 108, 187
TspMI CCCGGG 1 cut(s) 79
TspRI CASTG 1 cut(s) 192
XmaI CCCGGG 1 cut(s) 79
Zsp2I ATGCAT 1 cut(s) 143
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.