Rmu_ssc0000446.1_g000003

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000446.1
OG0018125
Physical Location & Seq
Reverse (-)
6074 .. 7072
999 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_ssc0000446.1_g000003.1.cds

Sequence Viewer

Length: 276 bp
atggtgaaccggtggaatatttgctacgagttgtatggagcttccggtggccggttcggtaagtttccggccggttcttggggtggggccgccggttctagactcctgggatcgattccttcaaggtgtgaggctaccaaagatcacagtggcaatctatcacttcttggtcgtcttgtggatgacattgtagactccttgcattctctgctaggggcttactttgtccatgtttatagggaatcaaatgttgcagcagataagcttgctaattag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

91

Amino Acids

9.76

Weight (kDa)

7.83

Isoelectric Point (pI)

22.29

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 192
AciI CCGC 1 cut(s) 90
AclWI GGATC 1 cut(s) 118
AcoI YGGCCR 2 cut(s) 49, 69
AfiI CCNNNNNNNGG 2 cut(s) 51, 78
AgeI ACCGGT 1 cut(s) 9
AgsI TTSAA 1 cut(s) 123
AjnI CCWGG 1 cut(s) 105
AluBI AGCT 2 cut(s) 41, 265
AluI AGCT 2 cut(s) 41, 265
AlwI GGATC 1 cut(s) 118
AoxI GGCC 3 cut(s) 49, 69, 87
ApeKI GCWGC 1 cut(s) 254
AsiGI ACCGGT 1 cut(s) 9
AspS9I GGNCC 1 cut(s) 87
AsuHPI GGTGA 1 cut(s) 16
BbvI GCAGC 1 cut(s) 266
BciT130I CCWGG 1 cut(s) 107
BfaI CTAG 2 cut(s) 99, 212
BisI GCNGC 2 cut(s) 90, 255
BlsI GCNGC 2 cut(s) 91, 256
Bme1390I CCNGG 1 cut(s) 107
BmgT120I GGNCC 1 cut(s) 87
BmiI GGNNCC 1 cut(s) 88
BmrFI CCNGG 1 cut(s) 107
Bsa29I ATCGAT 1 cut(s) 113
BsaJI CCNNGG 1 cut(s) 106
BsaWI WCCGGW 2 cut(s) 9, 44
Bsc4I CCNNNNNNNGG 2 cut(s) 51, 78
Bse118I RCCGGY 4 cut(s) 9, 51, 71, 92
BseBI CCWGG 1 cut(s) 107
BseCI ATCGAT 1 cut(s) 113
BseDI CCNNGG 1 cut(s) 106
BseGI GGATG 1 cut(s) 187
BseLI CCNNNNNNNGG 2 cut(s) 51, 78
BseX3I CGGCCG 1 cut(s) 69
BseXI GCAGC 1 cut(s) 266
Bsh1285I CGRYCG 1 cut(s) 72
BshFI GGCC 3 cut(s) 51, 71, 89
BshTI ACCGGT 1 cut(s) 9
BshVI ATCGAT 1 cut(s) 113
BsiEI CGRYCG 1 cut(s) 72
BsiSI CCGG 6 cut(s) 10, 45, 52, 68, 72, 93
BslI CCNNNNNNNGG 2 cut(s) 51, 78
BsmI GAATGC 1 cut(s) 202
BsnI GGCC 3 cut(s) 51, 71, 89
Bsp143I GATC 2 cut(s) 110, 142
BspACI CCGC 1 cut(s) 90
BspANI GGCC 3 cut(s) 51, 71, 89
BspDI ATCGAT 1 cut(s) 113
BspLI GGNNCC 1 cut(s) 88
BspPI GGATC 1 cut(s) 118
BsrFI RCCGGY 4 cut(s) 9, 51, 71, 92
BssAI RCCGGY 4 cut(s) 9, 51, 71, 92
BssECI CCNNGG 1 cut(s) 106
BssMI GATC 2 cut(s) 110, 142
Bst2UI CCWGG 1 cut(s) 107
Bst4CI ACNGT 1 cut(s) 149
BstAPI GCANNNNNTGC 1 cut(s) 208
BstC8I GCNNGC 1 cut(s) 267
BstF5I GGATG 1 cut(s) 187
BstKTI GATC 2 cut(s) 113, 145
BstMBI GATC 2 cut(s) 110, 142
BstMCI CGRYCG 1 cut(s) 72
BstMWI GCNNNNNNNGC 1 cut(s) 208
BstNI CCWGG 1 cut(s) 107
BstSCI CCNGG 1 cut(s) 105
BstV1I GCAGC 1 cut(s) 266
BstZI CGGCCG 1 cut(s) 69
Bsu15I ATCGAT 1 cut(s) 113
BsuRI GGCC 3 cut(s) 51, 71, 89
BsuTUI ATCGAT 1 cut(s) 113
BtsCI GGATG 1 cut(s) 187
BtsIMutI CAGTG 1 cut(s) 154
Cac8I GCNNGC 1 cut(s) 267
Cfr10I RCCGGY 4 cut(s) 9, 51, 71, 92
Cfr13I GGNCC 1 cut(s) 87
ClaI ATCGAT 1 cut(s) 113
CspAI ACCGGT 1 cut(s) 9
CviAII CATG 1 cut(s) 230
CviJI RGCY 7 cut(s) 41, 51, 71, 89, 134, 218, 265
CviKI_1 RGCY 7 cut(s) 41, 51, 71, 89, 134, 218, 265
DpnI GATC 2 cut(s) 112, 144
DpnII GATC 2 cut(s) 110, 142
EaeI YGGCCR 2 cut(s) 49, 69
EagI CGGCCG 1 cut(s) 69
EclXI CGGCCG 1 cut(s) 69
Eco52I CGGCCG 1 cut(s) 69
EcoRII CCWGG 1 cut(s) 105
FaeI CATG 1 cut(s) 233
FaiI YATR 3 cut(s) 36, 231, 237
FatI CATG 1 cut(s) 229
FblI GTMKAC 1 cut(s) 192
Fnu4HI GCNGC 2 cut(s) 90, 255
FokI GGATG 1 cut(s) 194
Fsp4HI GCNGC 2 cut(s) 90, 255
FspBI CTAG 2 cut(s) 99, 212
GluI GCNGC 2 cut(s) 90, 255
HaeIII GGCC 3 cut(s) 51, 71, 89
HapII CCGG 6 cut(s) 10, 45, 52, 68, 72, 93
Hin1II CATG 1 cut(s) 233
HindIII AAGCTT 1 cut(s) 263
HinfI GANTC 4 cut(s) 102, 115, 194, 242
HpaII CCGG 6 cut(s) 10, 45, 52, 68, 72, 93
HphI GGTGA 1 cut(s) 16
Hpy166II GTNNAC 2 cut(s) 7, 193
Hpy188III TCNNGA 1 cut(s) 99
Hpy8I GTNNAC 2 cut(s) 7, 193
HpyAV CCTTC 1 cut(s) 129
HpyCH4III ACNGT 1 cut(s) 149
HpyCH4V TGCA 2 cut(s) 202, 254
HpyF10VI GCNNNNNNNGC 1 cut(s) 208
Hsp92II CATG 1 cut(s) 233
Kzo9I GATC 2 cut(s) 110, 142
LmnI GCTCC 1 cut(s) 38
LpnPI CCDG 8 cut(s) 23, 58, 65, 81, 85, 92, 106, 119
Lsp1109I GCAGC 1 cut(s) 266
MaeI CTAG 2 cut(s) 99, 212
MalI GATC 2 cut(s) 112, 144
MboI GATC 2 cut(s) 110, 142
MluCI AATT 1 cut(s) 271
MlyI GAGTC 2 cut(s) 96, 188
MnlI CCTC 1 cut(s) 124
MspI CCGG 6 cut(s) 10, 45, 52, 68, 72, 93
MspR9I CCNGG 1 cut(s) 107
Mva1269I GAATGC 1 cut(s) 202
MvaI CCWGG 1 cut(s) 107
MwoI GCNNNNNNNGC 1 cut(s) 208
NdeII GATC 2 cut(s) 110, 142
NlaIII CATG 1 cut(s) 233
NlaIV GGNNCC 1 cut(s) 88
PctI GAATGC 1 cut(s) 202
PfeI GAWTC 2 cut(s) 115, 242
PinAI ACCGGT 1 cut(s) 9
PkrI GCNGC 2 cut(s) 91, 256
PleI GAGTC 2 cut(s) 96, 188
PpsI GAGTC 2 cut(s) 96, 188
Psp6I CCWGG 1 cut(s) 105
PspGI CCWGG 1 cut(s) 105
PspN4I GGNNCC 1 cut(s) 88
PspPI GGNCC 1 cut(s) 87
SatI GCNGC 2 cut(s) 90, 255
Sau3AI GATC 2 cut(s) 110, 142
Sau96I GGNCC 1 cut(s) 87
SchI GAGTC 2 cut(s) 96, 188
ScrFI CCNGG 1 cut(s) 107
SetI ASST 3 cut(s) 43, 128, 267
Sse9I AATT 1 cut(s) 271
SsiI CCGC 1 cut(s) 90
SspI AATATT 1 cut(s) 19
SspMI CTAG 2 cut(s) 99, 212
StyD4I CCNGG 1 cut(s) 105
TaaI ACNGT 1 cut(s) 149
TaqI TCGA 1 cut(s) 113
TasI AATT 1 cut(s) 271
TauI GCSGC 1 cut(s) 92
TfiI GAWTC 2 cut(s) 115, 242
TscAI CASTG 1 cut(s) 154
TseI GCWGC 1 cut(s) 254
TspRI CASTG 1 cut(s) 154
XbaI TCTAGA 1 cut(s) 98
XmiI GTMKAC 1 cut(s) 192
XspI CTAG 2 cut(s) 99, 212
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.