Rmu_sc0001647.1_g000001

Tricin synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001647.1
OG0004057
Physical Location & Seq
Reverse (-)
1 .. 2949
2949 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 747 bp
atggggagcaccagcttggggcttcatcggtggcctatcttagctcgtcagcgagtacttattccgcaaagatctgtgcaactacaaccgaagttttattctttttcctgggagaggtgtcatagaggtggcattggcttcagggcttgcagcagtaagttaaagaagagcattagaggttgtgccaccaatgcatttgttgttgctgatgataaaaagtatgggaacaagcagattatcagtattactccgcggctttatgattacatccttgccaatgtacgagagccagaggttctacgacaacttagggaggagactgcctccatgcgtggtagtatgatgcaggtgtctcctgaccaggcacagctgcttgcaatgcttgtgcagattcatggggcagaaaagtgtattgaagttggttctgccgttctgctctgccacattgtatttatggggagcaccagcttggggcttcatcggtggcctatcttagctcgtcagcgagtacttcttccgcaaagatctgcgcaactacgaccgaagttttattctttgtcctgggagaggtgtcatagaggtggcattggcttcagggcttgcagcagtaagttaaagaagagcattagaggttgtgccaccaatgcatttgttgttgctgatgataaaaagtatgggaacaagcagattatcagtattactccgcggctttatgattacatccttgccaatgtacgagagccagag

Protein Analysis

249

Amino Acids

28.22

Weight (kDa)

10.13

Isoelectric Point (pI)

48.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_3 PF01596 94 - 142 1.4e-09 O-methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 337
Acc16I TGCGCA 1 cut(s) 531
Acc36I ACCTGC 1 cut(s) 337
AccII CGCG 2 cut(s) 253, 706
AciI CCGC 6 cut(s) 65, 251, 253, 518, 704, 706
AcuI CTGAAG 2 cut(s) 124, 577
AfaI GTAC 4 cut(s) 57, 282, 510, 735
AfiI CCNNNNNNNGG 4 cut(s) 18, 114, 471, 567
AgsI TTSAA 1 cut(s) 416
AjnI CCWGG 3 cut(s) 107, 360, 560
AluBI AGCT 5 cut(s) 15, 44, 370, 468, 497
AluI AGCT 5 cut(s) 15, 44, 370, 468, 497
Alw21I GWGCWC 2 cut(s) 11, 464
Alw26I GTCTC 2 cut(s) 311, 357
AoxI GGCC 2 cut(s) 32, 485
ApeKI GCWGC 3 cut(s) 150, 370, 603
AspLEI GCGC 1 cut(s) 532
Bbv12I GWGCWC 2 cut(s) 11, 464
BbvI GCAGC 3 cut(s) 162, 357, 615
BceAI ACGGC 1 cut(s) 413
BciT130I CCWGG 3 cut(s) 109, 362, 562
BcoDI GTCTC 2 cut(s) 311, 357
BfuAI ACCTGC 1 cut(s) 337
BglII AGATCT 2 cut(s) 71, 524
BisI GCNGC 5 cut(s) 151, 254, 371, 604, 707
BlsI GCNGC 5 cut(s) 152, 255, 372, 605, 708
BmcAI AGTACT 2 cut(s) 57, 510
Bme1390I CCNGG 3 cut(s) 109, 362, 562
BmrFI CCNGG 3 cut(s) 109, 362, 562
BmsI GCATC 1 cut(s) 333
BplI GAGNNNNNCTC 2 cut(s) 308, 340
BsaJI CCNNGG 4 cut(s) 108, 251, 561, 704
Bsc4I CCNNNNNNNGG 4 cut(s) 18, 114, 471, 567
Bse3DI GCAATG 1 cut(s) 384
BseBI CCWGG 3 cut(s) 109, 362, 562
BseDI CCNNGG 4 cut(s) 108, 251, 561, 704
BseGI GGATG 2 cut(s) 267, 720
BseLI CCNNNNNNNGG 4 cut(s) 18, 114, 471, 567
BseMI GCAATG 1 cut(s) 384
BseRI GAGGAG 1 cut(s) 329
BseXI GCAGC 3 cut(s) 162, 357, 615
BsgI GTGCAG 1 cut(s) 407
Bsh1236I CGCG 2 cut(s) 253, 706
Bsh1285I CGRYCG 1 cut(s) 542
BshFI GGCC 2 cut(s) 34, 487
BsiEI CGRYCG 1 cut(s) 542
BsiHKAI GWGCWC 2 cut(s) 11, 464
BslI CCNNNNNNNGG 4 cut(s) 18, 114, 471, 567
BsmAI GTCTC 2 cut(s) 311, 357
BsnI GGCC 2 cut(s) 34, 487
Bsp1286I GDGCHC 2 cut(s) 11, 464
Bsp143I GATC 2 cut(s) 71, 524
BspACI CCGC 6 cut(s) 65, 251, 253, 518, 704, 706
BspANI GGCC 2 cut(s) 34, 487
BspFNI CGCG 2 cut(s) 253, 706
BspMI ACCTGC 1 cut(s) 337
BspQI GCTCTTC 2 cut(s) 161, 614
BsrDI GCAATG 1 cut(s) 384
BssECI CCNNGG 4 cut(s) 108, 251, 561, 704
BssMI GATC 2 cut(s) 71, 524
Bst2UI CCWGG 3 cut(s) 109, 362, 562
Bst6I CTCTTC 2 cut(s) 161, 614
BstC8I GCNNGC 3 cut(s) 148, 375, 601
BstDEI CTNAG 3 cut(s) 40, 308, 493
BstDSI CCRYGG 2 cut(s) 251, 704
BstENI CCTNNNNNAGG 2 cut(s) 112, 565
BstF5I GGATG 2 cut(s) 267, 720
BstFNI CGCG 2 cut(s) 253, 706
BstHHI GCGC 1 cut(s) 532
BstKTI GATC 2 cut(s) 74, 527
BstMAI GTCTC 2 cut(s) 311, 357
BstMBI GATC 2 cut(s) 71, 524
BstMCI CGRYCG 1 cut(s) 542
BstMWI GCNNNNNNNGC 3 cut(s) 191, 379, 644
BstNI CCWGG 3 cut(s) 109, 362, 562
BstSCI CCNGG 3 cut(s) 107, 360, 560
BstUI CGCG 2 cut(s) 253, 706
BstV1I GCAGC 3 cut(s) 162, 357, 615
BstX2I RGATCY 2 cut(s) 71, 524
BstYI RGATCY 2 cut(s) 71, 524
BsuRI GGCC 2 cut(s) 34, 487
BtgI CCRYGG 2 cut(s) 251, 704
BtsCI GGATG 2 cut(s) 267, 720
BveI ACCTGC 1 cut(s) 337
Cac8I GCNNGC 3 cut(s) 148, 375, 601
CfoI GCGC 1 cut(s) 532
Cfr42I CCGCGG 2 cut(s) 254, 707
Csp6I GTAC 4 cut(s) 56, 281, 509, 734
CviAII CATG 2 cut(s) 328, 395
CviQI GTAC 4 cut(s) 56, 281, 509, 734
DdeI CTNAG 3 cut(s) 40, 308, 493
DpnI GATC 2 cut(s) 73, 526
DpnII GATC 2 cut(s) 71, 524
Eam1104I CTCTTC 2 cut(s) 161, 614
EarI CTCTTC 2 cut(s) 161, 614
Eco57I CTGAAG 2 cut(s) 124, 577
EcoNI CCTNNNNNAGG 2 cut(s) 112, 565
EcoRII CCWGG 3 cut(s) 107, 360, 560
EcoT22I ATGCAT 2 cut(s) 196, 649
FaeI CATG 2 cut(s) 331, 398
FatI CATG 2 cut(s) 327, 394
Fnu4HI GCNGC 5 cut(s) 151, 254, 371, 604, 707
FokI GGATG 2 cut(s) 254, 707
Fsp4HI GCNGC 5 cut(s) 151, 254, 371, 604, 707
FspI TGCGCA 1 cut(s) 531
GlaI GCGC 1 cut(s) 531
GluI GCNGC 5 cut(s) 151, 254, 371, 604, 707
HaeIII GGCC 2 cut(s) 34, 487
HhaI GCGC 1 cut(s) 532
Hin1II CATG 2 cut(s) 331, 398
Hin6I GCGC 1 cut(s) 530
HinP1I GCGC 1 cut(s) 530
HinfI GANTC 1 cut(s) 391
Hpy188III TCNNGA 1 cut(s) 356
HpyCH4V TGCA 8 cut(s) 79, 150, 194, 346, 377, 388, 603, 647
HpyF10VI GCNNNNNNNGC 3 cut(s) 191, 379, 644
HpyF3I CTNAG 3 cut(s) 40, 308, 493
Hsp92II CATG 2 cut(s) 331, 398
HspAI GCGC 1 cut(s) 530
KspI CCGCGG 2 cut(s) 254, 707
Kzo9I GATC 2 cut(s) 71, 524
LguI GCTCTTC 2 cut(s) 161, 614
LmnI GCTCC 2 cut(s) 6, 459
Lsp1109I GCAGC 3 cut(s) 162, 357, 615
LweI GCATC 1 cut(s) 333
MalI GATC 2 cut(s) 73, 526
MboI GATC 2 cut(s) 71, 524
MboII GAAGA 3 cut(s) 178, 506, 631
MflI RGATCY 2 cut(s) 71, 524
MhlI GDGCHC 2 cut(s) 11, 464
MnlI CCTC 9 cut(s) 108, 119, 170, 286, 307, 334, 561, 572, 623
Mph1103I ATGCAT 2 cut(s) 196, 649
MseI TTAA 2 cut(s) 161, 614
MslI CAYNNNNRTG 2 cut(s) 126, 579
MspA1I CMGCKG 3 cut(s) 253, 370, 706
MspR9I CCNGG 3 cut(s) 109, 362, 562
MvaI CCWGG 3 cut(s) 109, 362, 562
MvnI CGCG 2 cut(s) 253, 706
MwoI GCNNNNNNNGC 3 cut(s) 191, 379, 644
NdeII GATC 2 cut(s) 71, 524
NlaIII CATG 2 cut(s) 331, 398
NsbI TGCGCA 1 cut(s) 531
NsiI ATGCAT 2 cut(s) 196, 649
PaqCI CACCTGC 1 cut(s) 337
PciSI GCTCTTC 2 cut(s) 161, 614
PfeI GAWTC 1 cut(s) 391
PkrI GCNGC 5 cut(s) 152, 255, 372, 605, 708
Psp6I CCWGG 3 cut(s) 107, 360, 560
PspGI CCWGG 3 cut(s) 107, 360, 560
PsuI RGATCY 2 cut(s) 71, 524
PvuII CAGCTG 1 cut(s) 370
RsaI GTAC 4 cut(s) 57, 282, 510, 735
RsaNI GTAC 4 cut(s) 56, 281, 509, 734
RseI CAYNNNNRTG 2 cut(s) 126, 579
SacII CCGCGG 2 cut(s) 254, 707
SapI GCTCTTC 2 cut(s) 161, 614
SaqAI TTAA 2 cut(s) 161, 614
SatI GCNGC 5 cut(s) 151, 254, 371, 604, 707
Sau3AI GATC 2 cut(s) 71, 524
ScaI AGTACT 2 cut(s) 57, 510
ScrFI CCNGG 3 cut(s) 109, 362, 562
SduI GDGCHC 2 cut(s) 11, 464
SfaNI GCATC 1 cut(s) 333
Sfr303I CCGCGG 2 cut(s) 254, 707
SgrBI CCGCGG 2 cut(s) 254, 707
SmiMI CAYNNNNRTG 2 cut(s) 126, 579
SsiI CCGC 6 cut(s) 65, 251, 253, 518, 704, 706
StyD4I CCNGG 3 cut(s) 107, 360, 560
TaqII GACCGA 1 cut(s) 556
TatI WGTACW 2 cut(s) 55, 508
TauI GCSGC 2 cut(s) 256, 709
TfiI GAWTC 1 cut(s) 391
Tru1I TTAA 2 cut(s) 161, 614
Tru9I TTAA 2 cut(s) 161, 614
TseI GCWGC 3 cut(s) 150, 370, 603
TspDTI ATGAA 3 cut(s) 14, 383, 467
XagI CCTNNNNNAGG 2 cut(s) 112, 565
ZrmI AGTACT 2 cut(s) 57, 510
Zsp2I ATGCAT 2 cut(s) 196, 649
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.