Rmu_sc0001650.1_g000001

tRNA pseudouridine

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001650.1
OG0004793
Physical Location & Seq
Forward (+)
1 .. 1899
1899 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0001650.1_g000001.1.cds

Sequence Viewer

Length: 477 bp
aaattcttgcaggaagcctgcaaaattctggttggacaccatgattttagttcctttagagcagcaggttgtcaggcgaagtcaccgatcagaacactagatgagttgaatgtgtctgaggttatctcaactccaaactttccaacaataccagagaggaaaagcaatttagtgcaggaagatactcattgcaccaacaaatctgagaccaactgccaccctagtttcaatggaggaacttatcaaggatttggcttgagaaaaaaacacagatgctttgtggtaacagctcgagcacgtgcttttctataccaccaggtaagactgcttgttggtgtcctcaaatgtgttggaactggagatctaaaaatacccgacgtggagaggatcttgaatgcaaagacagtgactgctgctagccccatggcacctgcgtgtggtctatatcttggacatgtgagatacgatttgccatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PseudoU_synth_1

Protein Analysis

158

Amino Acids

17.45

Weight (kDa)

9.17

Isoelectric Point (pI)

35.48

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 439
Acc36I ACCTGC 2 cut(s) 56, 439
AccB1I GGYRCC 1 cut(s) 427
AclWI GGATC 1 cut(s) 395
AcsI RAATTY 2 cut(s) 2, 24
AcvI CACGTG 1 cut(s) 299
AfiI CCNNNNNNNGG 1 cut(s) 437
AflIII ACRYGT 1 cut(s) 454
AgsI TTSAA 3 cut(s) 109, 229, 394
AjiI CACGTC 1 cut(s) 379
AjnI CCWGG 1 cut(s) 315
AleI CACNNNNGTG 1 cut(s) 433
AluBI AGCT 1 cut(s) 290
AluI AGCT 1 cut(s) 290
Alw21I GWGCWC 1 cut(s) 298
Alw26I GTCTC 1 cut(s) 200
AlwI GGATC 1 cut(s) 395
AlwNI CAGNNNCTG 1 cut(s) 410
Ama87I CYCGRG 1 cut(s) 291
ApeKI GCWGC 2 cut(s) 62, 413
ApoI RAATTY 2 cut(s) 2, 24
AsuHPI GGTGA 1 cut(s) 75
AsuNHI GCTAGC 1 cut(s) 416
AvaI CYCGRG 1 cut(s) 291
BanI GGYRCC 1 cut(s) 427
BbrPI CACGTG 1 cut(s) 299
Bbv12I GWGCWC 1 cut(s) 298
BbvI GCAGC 2 cut(s) 74, 400
BciT130I CCWGG 1 cut(s) 317
BcoDI GTCTC 1 cut(s) 200
BfaI CTAG 3 cut(s) 98, 222, 417
BfuAI ACCTGC 2 cut(s) 56, 439
BglII AGATCT 1 cut(s) 361
BisI GCNGC 2 cut(s) 63, 414
BlsI GCNGC 2 cut(s) 64, 415
Bme1390I CCNGG 1 cut(s) 317
BmeT110I CYCGRG 1 cut(s) 291
BmgBI CACGTC 1 cut(s) 379
BmiI GGNNCC 1 cut(s) 429
BmrFI CCNGG 1 cut(s) 317
BmsI GCATC 1 cut(s) 263
BmtI GCTAGC 1 cut(s) 420
BplI GAGNNNNNCTC 2 cut(s) 110, 142
BpmI CTGGAG 1 cut(s) 378
BpuEI CTTGAG 1 cut(s) 277
BsaAI YACGTR 1 cut(s) 299
BsaI GGTCTC 1 cut(s) 200
BsaJI CCNNGG 1 cut(s) 423
Bsc4I CCNNNNNNNGG 1 cut(s) 437
Bse1I ACTGG 1 cut(s) 361
Bse3DI GCAATG 1 cut(s) 187
BseBI CCWGG 1 cut(s) 317
BseDI CCNNGG 1 cut(s) 423
BseLI CCNNNNNNNGG 1 cut(s) 437
BseMI GCAATG 1 cut(s) 187
BseMII CTCAG 2 cut(s) 108, 195
BseNI ACTGG 1 cut(s) 361
BseXI GCAGC 2 cut(s) 74, 400
BsgI GTGCAG 1 cut(s) 194
BshNI GGYRCC 1 cut(s) 427
BsiHKAI GWGCWC 1 cut(s) 298
BsiHKCI CYCGRG 1 cut(s) 291
BslI CCNNNNNNNGG 1 cut(s) 437
BsmAI GTCTC 1 cut(s) 200
BsmI GAATGC 1 cut(s) 400
Bso31I GGTCTC 1 cut(s) 200
BsoBI CYCGRG 1 cut(s) 291
Bsp1286I GDGCHC 1 cut(s) 298
Bsp143I GATC 3 cut(s) 87, 361, 387
Bsp19I CCATGG 1 cut(s) 423
BspCNI CTCAG 2 cut(s) 109, 196
BspLI GGNNCC 1 cut(s) 429
BspMI ACCTGC 2 cut(s) 56, 439
BspOI GCTAGC 1 cut(s) 420
BspPI GGATC 1 cut(s) 395
BspT107I GGYRCC 1 cut(s) 427
BspTNI GGTCTC 1 cut(s) 200
BsrDI GCAATG 1 cut(s) 187
BsrI ACTGG 1 cut(s) 361
BssECI CCNNGG 1 cut(s) 423
BssMI GATC 3 cut(s) 87, 361, 387
BssT1I CCWWGG 1 cut(s) 423
Bst2UI CCWGG 1 cut(s) 317
Bst4CI ACNGT 1 cut(s) 406
BstBAI YACGTR 1 cut(s) 299
BstC8I GCNNGC 2 cut(s) 19, 418
BstDEI CTNAG 2 cut(s) 117, 204
BstDSI CCRYGG 1 cut(s) 423
BstKTI GATC 3 cut(s) 90, 364, 390
BstMAI GTCTC 1 cut(s) 200
BstMBI GATC 3 cut(s) 87, 361, 387
BstNI CCWGG 1 cut(s) 317
BstNSI RCATGY 1 cut(s) 458
BstSCI CCNGG 1 cut(s) 315
BstV1I GCAGC 2 cut(s) 74, 400
BstX2I RGATCY 2 cut(s) 361, 387
BstYI RGATCY 2 cut(s) 361, 387
BtgI CCRYGG 1 cut(s) 423
BtrI CACGTC 1 cut(s) 379
BtsIMutI CAGTG 1 cut(s) 411
BveI ACCTGC 2 cut(s) 56, 439
Cac8I GCNNGC 2 cut(s) 19, 418
CaiI CAGNNNCTG 1 cut(s) 410
CsiI ACCWGGT 1 cut(s) 315
CviAII CATG 4 cut(s) 41, 424, 455, 474
CviJI RGCY 4 cut(s) 17, 255, 290, 420
CviKI_1 RGCY 4 cut(s) 17, 255, 290, 420
DdeI CTNAG 2 cut(s) 117, 204
DpnI GATC 3 cut(s) 89, 363, 389
DpnII GATC 3 cut(s) 87, 361, 387
Eco130I CCWWGG 1 cut(s) 423
Eco31I GGTCTC 1 cut(s) 200
Eco72I CACGTG 1 cut(s) 299
Eco88I CYCGRG 1 cut(s) 291
EcoRII CCWGG 1 cut(s) 315
EcoT14I CCWWGG 1 cut(s) 423
ErhI CCWWGG 1 cut(s) 423
FaeI CATG 4 cut(s) 44, 427, 458, 477
FaiI YATR 6 cut(s) 42, 310, 425, 445, 456, 475
FatI CATG 4 cut(s) 40, 423, 454, 473
Fnu4HI GCNGC 2 cut(s) 63, 414
Fsp4HI GCNGC 2 cut(s) 63, 414
FspBI CTAG 3 cut(s) 98, 222, 417
GluI GCNGC 2 cut(s) 63, 414
GsuI CTGGAG 1 cut(s) 378
Hin1II CATG 4 cut(s) 44, 427, 458, 477
HphI GGTGA 1 cut(s) 75
Hpy188I TCNGA 3 cut(s) 92, 118, 205
Hpy188III TCNNGA 1 cut(s) 391
Hpy99I CGWCG 1 cut(s) 380
HpyCH4III ACNGT 1 cut(s) 406
HpyCH4IV ACGT 2 cut(s) 298, 378
HpyCH4V TGCA 5 cut(s) 10, 21, 175, 192, 398
HpyF3I CTNAG 2 cut(s) 117, 204
HpySE526I ACGT 2 cut(s) 298, 378
Hsp92II CATG 4 cut(s) 44, 427, 458, 477
Kzo9I GATC 3 cut(s) 87, 361, 387
Lsp1109I GCAGC 2 cut(s) 74, 400
LweI GCATC 1 cut(s) 263
MabI ACCWGGT 1 cut(s) 315
MaeI CTAG 3 cut(s) 98, 222, 417
MaeII ACGT 2 cut(s) 298, 378
MaeIII GTNAC 3 cut(s) 81, 283, 406
MalI GATC 3 cut(s) 89, 363, 389
MboI GATC 3 cut(s) 87, 361, 387
MboII GAAGA 1 cut(s) 191
MflI RGATCY 2 cut(s) 361, 387
MhlI GDGCHC 1 cut(s) 298
MluCI AATT 3 cut(s) 2, 24, 166
MmeI TCCRAC 3 cut(s) 13, 167, 331
MnlI CCTC 5 cut(s) 112, 150, 227, 350, 378
MslI CAYNNNNRTG 1 cut(s) 433
MspR9I CCNGG 1 cut(s) 317
Mva1269I GAATGC 1 cut(s) 400
MvaI CCWGG 1 cut(s) 317
NcoI CCATGG 1 cut(s) 423
NdeII GATC 3 cut(s) 87, 361, 387
NheI GCTAGC 1 cut(s) 416
NlaIII CATG 4 cut(s) 44, 427, 458, 477
NlaIV GGNNCC 1 cut(s) 429
NmuCI GTSAC 2 cut(s) 81, 406
NspI RCATGY 1 cut(s) 458
OliI CACNNNNGTG 1 cut(s) 433
PaeR7I CTCGAG 1 cut(s) 291
PaqCI CACCTGC 1 cut(s) 439
PciI ACATGT 1 cut(s) 454
PctI GAATGC 1 cut(s) 400
PkrI GCNGC 2 cut(s) 64, 415
PmaCI CACGTG 1 cut(s) 299
PmlI CACGTG 1 cut(s) 299
Ppu21I YACGTR 1 cut(s) 299
PscI ACATGT 1 cut(s) 454
Psp6I CCWGG 1 cut(s) 315
PspCI CACGTG 1 cut(s) 299
PspGI CCWGG 1 cut(s) 315
PspN4I GGNNCC 1 cut(s) 429
PspXI VCTCGAGB 1 cut(s) 291
PstNI CAGNNNCTG 1 cut(s) 410
PsuI RGATCY 2 cut(s) 361, 387
RseI CAYNNNNRTG 1 cut(s) 433
SatI GCNGC 2 cut(s) 63, 414
Sau3AI GATC 3 cut(s) 87, 361, 387
ScrFI CCNGG 1 cut(s) 317
SduI GDGCHC 1 cut(s) 298
SetI ASST 7 cut(s) 70, 123, 292, 301, 321, 381, 433
SexAI ACCWGGT 1 cut(s) 315
SfaNI GCATC 1 cut(s) 263
Sfr274I CTCGAG 1 cut(s) 291
SlaI CTCGAG 1 cut(s) 291
SmiMI CAYNNNNRTG 1 cut(s) 433
SmlI CTYRAG 2 cut(s) 256, 291
SmoI CTYRAG 2 cut(s) 256, 291
Sse9I AATT 3 cut(s) 2, 24, 166
SspMI CTAG 3 cut(s) 98, 222, 417
StyD4I CCNGG 1 cut(s) 315
StyI CCWWGG 1 cut(s) 423
TaaI ACNGT 1 cut(s) 406
TaiI ACGT 2 cut(s) 301, 381
TaqI TCGA 1 cut(s) 292
TasI AATT 3 cut(s) 2, 24, 166
TscAI CASTG 1 cut(s) 411
TseFI GTSAC 2 cut(s) 81, 406
TseI GCWGC 2 cut(s) 62, 413
Tsp45I GTSAC 2 cut(s) 81, 406
TspRI CASTG 1 cut(s) 411
XapI RAATTY 2 cut(s) 2, 24
XceI RCATGY 1 cut(s) 458
XhoI CTCGAG 1 cut(s) 291
XspI CTAG 3 cut(s) 98, 222, 417
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.