Rmu_sc0001785.1_g000031

Uncharacterised protein family UPF0066

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001785.1
OG0002784
Physical Location & Seq
Forward (+)
112804 .. 121236
8433 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1326 bp
atggctgaccatgccgggctgggtccaacccggtccaatttacaggtctatgatgtgaccgtgcgacaaggaactctggtctggtcaatgtgttttcaaaaacccacaacaaaaccccaacacagactccatagactcgaagagcaagaagccatgggtttcgctacagaatccaaatggttaagagccaccatagccgtcgcactcgcagctctctccgcctccactgctctttctctgtatttcttgaaaagaaaatccaaggatctggactccaagatcagggagctcgagaaatccctcaaggactccttaaagcactgtgcttccgaaaggcaaggtcgaattcgggctcagcaggctttgaggaaatcgctgacggaaccaaaggttgatgatttggagctgacttcttaccccatggcacctattggtgtcatccagtcatgcttctccaccaggaatgggacacccaggcaaccattaattgtacctctttccagggcatgcttgatctttaattcagcccgtgttcctccagcttcgcttgagggtcttggagactacacgcactgttgggttatctatgttttccatctaaatacaaatctagagaagctttggaaggatccagcaaaatcgaagtttaaggccaaggtgagagtgccaagactaaaaggtgaacgcatgggagtctttgccacccgatcaccacacaggccatgccccattgggctcactgttgcaaaggtggaggctgtaaaagggaatatacttctactctctggtgtggatttggttgatgggacgcctgtacttgatgtcaaaccgtatcttccttactgtgacagcatccaagaagcaggagtgccaaagtggttaacggtagacaggtcattttcagtggcttctattagtttctctgagggcttcacttcaacacttgctcaatgttgggccataacaggtaagaattcactgtacgcatcaccagatgagttccaaaacttgttagagcaggtgctgtcatgggatattcgatcagtatctcagcgaacacgtcctcatgagtccttcatcacatcccaaaatggtgaccactcgaatgatctctcagatgactaccaagacgaagaagcttctagccccggaaacgagcagcctccgcaatcatctggcgacataatttatcacctaattctagaaggattggatgtttggtacaggctctgtgatgggaatgttgttgttgagaaagtgacagaagcatcaaccgtcattaaaagtaatcaaaagcgatgtaattactctatatggagagactga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
UPF0066

Protein Analysis

441

Amino Acids

49.31

Weight (kDa)

8.56

Isoelectric Point (pI)

40.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TrmO_N PF01980 156 - 283 3.8e-38 tRNA-methyltransferase O N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1009
Acc36I ACCTGC 1 cut(s) 1009
AccB1I GGYRCC 1 cut(s) 424
AccI GTMKAC 1 cut(s) 888
AciI CCGC 2 cut(s) 219, 1166
AclWI GGATC 3 cut(s) 273, 623, 636
AcsI RAATTY 2 cut(s) 345, 973
AcyI GRCGYC 1 cut(s) 809
AfaI GTAC 4 cut(s) 492, 816, 983, 1223
AfiI CCNNNNNNNGG 2 cut(s) 282, 465
AflIII ACRYGT 1 cut(s) 1058
AgsI TTSAA 3 cut(s) 98, 250, 939
AjiI CACGTC 1 cut(s) 1061
AjnI CCWGG 3 cut(s) 458, 473, 500
AluBI AGCT 6 cut(s) 212, 289, 406, 542, 619, 1139
AluI AGCT 6 cut(s) 212, 289, 406, 542, 619, 1139
Alw21I GWGCWC 1 cut(s) 291
Alw26I GTCTC 2 cut(s) 555, 1314
AlwI GGATC 3 cut(s) 273, 623, 636
AlwNI CAGNNNCTG 2 cut(s) 1024, 1230
Ama87I CYCGRG 1 cut(s) 290
AoxI GGCC 3 cut(s) 651, 719, 957
ApeKI GCWGC 2 cut(s) 209, 1159
ApoI RAATTY 2 cut(s) 345, 973
AseI ATTAAT 1 cut(s) 485
AspS9I GGNCC 3 cut(s) 23, 33, 957
AsuC2I CCSGG 3 cut(s) 16, 31, 1149
AsuHPI GGTGA 6 cut(s) 670, 692, 702, 981, 1106, 1184
AvaI CYCGRG 1 cut(s) 290
AvaII GGWCC 2 cut(s) 23, 33
BamHI GGATCC 1 cut(s) 628
BanI GGYRCC 1 cut(s) 424
BanII GRGCYC 3 cut(s) 291, 355, 738
Bbv12I GWGCWC 1 cut(s) 291
BbvI GCAGC 2 cut(s) 221, 1171
BccI CCATC 3 cut(s) 603, 797, 1229
BceAI ACGGC 1 cut(s) 182
BciT130I CCWGG 3 cut(s) 460, 475, 502
BcnI CCSGG 3 cut(s) 16, 31, 1149
BcoDI GTCTC 2 cut(s) 555, 1314
BfaI CTAG 3 cut(s) 611, 1143, 1202
BfmI CTRYAG 1 cut(s) 165
BfuAI ACCTGC 1 cut(s) 1009
BisI GCNGC 2 cut(s) 210, 1160
BlpI GCTNAGC 1 cut(s) 354
BlsI GCNGC 2 cut(s) 211, 1161
Bme1390I CCNGG 6 cut(s) 16, 31, 460, 475, 502, 1149
Bme18I GGWCC 2 cut(s) 23, 33
BmeT110I CYCGRG 1 cut(s) 290
BmgBI CACGTC 1 cut(s) 1061
BmgT120I GGNCC 3 cut(s) 23, 33, 957
BmiI GGNNCC 4 cut(s) 24, 384, 426, 630
BmrFI CCNGG 6 cut(s) 16, 31, 460, 475, 502, 1149
BmsI GCATC 3 cut(s) 861, 995, 1277
BpmI CTGGAG 1 cut(s) 522
Bpu1102I GCTNAGC 1 cut(s) 354
BpuEI CTTGAG 2 cut(s) 287, 569
BpuMI CCSGG 3 cut(s) 16, 31, 1149
BsaBI GATNNNNATC 1 cut(s) 1045
BsaHI GRCGYC 1 cut(s) 809
BsaJI CCNNGG 7 cut(s) 153, 261, 420, 473, 501, 654, 1147
BsaXI ACNNNNNCTCC 2 cut(s) 111, 141
Bsc4I CCNNNNNNNGG 2 cut(s) 282, 465
Bse1I ACTGG 1 cut(s) 442
Bse8I GATNNNNATC 1 cut(s) 1045
BseBI CCWGG 3 cut(s) 460, 475, 502
BseDI CCNNGG 7 cut(s) 153, 261, 420, 473, 501, 654, 1147
BseGI GGATG 4 cut(s) 438, 852, 1082, 1219
BseJI GATNNNNATC 1 cut(s) 1045
BseLI CCNNNNNNNGG 2 cut(s) 282, 465
BseMII CTCAG 4 cut(s) 368, 915, 1064, 1128
BseNI ACTGG 1 cut(s) 442
BseXI GCAGC 2 cut(s) 221, 1171
BseYI CCCAGC 1 cut(s) 19
BshFI GGCC 3 cut(s) 653, 721, 959
BshNI GGYRCC 1 cut(s) 424
BsiHKAI GWGCWC 1 cut(s) 291
BsiHKCI CYCGRG 1 cut(s) 290
BsiSI CCGG 3 cut(s) 15, 31, 1149
BslFI GGGAC 2 cut(s) 481, 820
BslI CCNNNNNNNGG 2 cut(s) 282, 465
BsmAI GTCTC 2 cut(s) 555, 1314
BsmFI GGGAC 2 cut(s) 481, 820
BsnI GGCC 3 cut(s) 653, 721, 959
BsoBI CYCGRG 1 cut(s) 290
Bsp1286I GDGCHC 3 cut(s) 291, 355, 738
Bsp143I GATC 7 cut(s) 265, 279, 513, 628, 707, 1040, 1108
Bsp1720I GCTNAGC 1 cut(s) 354
Bsp19I CCATGG 2 cut(s) 153, 420
BspACI CCGC 2 cut(s) 219, 1166
BspANI GGCC 3 cut(s) 653, 721, 959
BspCNI CTCAG 4 cut(s) 367, 916, 1063, 1127
BspHI TCATGA 1 cut(s) 1066
BspLI GGNNCC 4 cut(s) 24, 384, 426, 630
BspMI ACCTGC 1 cut(s) 1009
BspPI GGATC 3 cut(s) 273, 623, 636
BspQI GCTCTTC 1 cut(s) 135
BspT107I GGYRCC 1 cut(s) 424
BsrI ACTGG 1 cut(s) 442
BssECI CCNNGG 7 cut(s) 153, 261, 420, 473, 501, 654, 1147
BssMI GATC 7 cut(s) 265, 279, 513, 628, 707, 1040, 1108
BssNI GRCGYC 1 cut(s) 809
BssT1I CCWWGG 4 cut(s) 153, 261, 420, 654
Bst2UI CCWGG 3 cut(s) 460, 475, 502
Bst4CI ACNGT 9 cut(s) 61, 323, 575, 742, 831, 845, 886, 981, 1276
Bst6I CTCTTC 1 cut(s) 135
BstACI GRCGYC 1 cut(s) 809
BstC8I GCNNGC 2 cut(s) 360, 508
BstDEI CTNAG 4 cut(s) 354, 924, 1050, 1114
BstDSI CCRYGG 2 cut(s) 153, 420
BstEII GGTNACC 1 cut(s) 1094
BstF5I GGATG 4 cut(s) 438, 852, 1082, 1219
BstKTI GATC 7 cut(s) 268, 282, 516, 631, 710, 1043, 1111
BstMAI GTCTC 2 cut(s) 555, 1314
BstMBI GATC 7 cut(s) 265, 279, 513, 628, 707, 1040, 1108
BstMWI GCNNNNNNNGC 7 cut(s) 11, 194, 209, 218, 227, 359, 1165
BstNI CCWGG 3 cut(s) 460, 475, 502
BstNSI RCATGY 1 cut(s) 510
BstPI GGTNACC 1 cut(s) 1094
BstSCI CCNGG 6 cut(s) 14, 29, 458, 473, 500, 1147
BstSFI CTRYAG 1 cut(s) 165
BstV1I GCAGC 2 cut(s) 221, 1171
BstX2I RGATCY 2 cut(s) 265, 628
BstXI CCANNNNNNTGG 1 cut(s) 268
BstYI RGATCY 2 cut(s) 265, 628
BsuRI GGCC 3 cut(s) 653, 721, 959
BtgI CCRYGG 2 cut(s) 153, 420
BtgZI GCGATG 1 cut(s) 1312
BtrI CACGTC 1 cut(s) 1061
BtsCI GGATG 4 cut(s) 438, 852, 1082, 1219
BtsI GCAGTG 1 cut(s) 225
BtsIMutI CAGTG 6 cut(s) 225, 319, 571, 738, 909, 977
BveI ACCTGC 1 cut(s) 1009
Cac8I GCNNGC 2 cut(s) 360, 508
CaiI CAGNNNCTG 2 cut(s) 1024, 1230
CciI TCATGA 1 cut(s) 1066
Cfr13I GGNCC 3 cut(s) 23, 33, 957
CseI GACGC 1 cut(s) 817
Csp6I GTAC 4 cut(s) 491, 815, 982, 1222
CviAII CATG 9 cut(s) 11, 154, 421, 447, 507, 688, 723, 1029, 1067
CviQI GTAC 4 cut(s) 491, 815, 982, 1222
DdeI CTNAG 4 cut(s) 354, 924, 1050, 1114
DpnI GATC 7 cut(s) 267, 281, 515, 630, 709, 1042, 1110
DpnII GATC 7 cut(s) 265, 279, 513, 628, 707, 1040, 1108
Eam1104I CTCTTC 1 cut(s) 135
EarI CTCTTC 1 cut(s) 135
EciI GGCGGA 1 cut(s) 208
Ecl136II GAGCTC 1 cut(s) 289
Eco130I CCWWGG 4 cut(s) 153, 261, 420, 654
Eco24I GRGCYC 3 cut(s) 291, 355, 738
Eco47I GGWCC 2 cut(s) 23, 33
Eco53kI GAGCTC 1 cut(s) 289
Eco88I CYCGRG 1 cut(s) 290
Eco91I GGTNACC 1 cut(s) 1094
EcoICRI GAGCTC 1 cut(s) 289
EcoO65I GGTNACC 1 cut(s) 1094
EcoRI GAATTC 2 cut(s) 345, 973
EcoRII CCWGG 3 cut(s) 458, 473, 500
EcoT14I CCWWGG 4 cut(s) 153, 261, 420, 654
EcoT38I GRGCYC 3 cut(s) 291, 355, 738
ErhI CCWWGG 4 cut(s) 153, 261, 420, 654
FaeI CATG 9 cut(s) 14, 157, 424, 450, 510, 691, 726, 1032, 1070
FalI AAGNNNNNCTT 2 cut(s) 296, 328
FaqI GGGAC 2 cut(s) 481, 820
FatI CATG 9 cut(s) 10, 153, 420, 446, 506, 687, 722, 1028, 1066
FblI GTMKAC 1 cut(s) 888
Fnu4HI GCNGC 2 cut(s) 210, 1160
FokI GGATG 4 cut(s) 425, 839, 1069, 1226
FriOI GRGCYC 3 cut(s) 291, 355, 738
Fsp4HI GCNGC 2 cut(s) 210, 1160
FspBI CTAG 3 cut(s) 611, 1143, 1202
GluI GCNGC 2 cut(s) 210, 1160
GsaI CCCAGC 1 cut(s) 23
GsuI CTGGAG 1 cut(s) 522
HaeIII GGCC 3 cut(s) 653, 721, 959
HapII CCGG 3 cut(s) 15, 31, 1149
HgaI GACGC 1 cut(s) 817
Hin1I GRCGYC 1 cut(s) 809
Hin1II CATG 9 cut(s) 14, 157, 424, 450, 510, 691, 726, 1032, 1070
HincII GTYRAC 1 cut(s) 882
HindII GTYRAC 1 cut(s) 882
HindIII AAGCTT 2 cut(s) 617, 1137
HinfI GANTC 7 cut(s) 126, 135, 170, 272, 308, 693, 1070
HpaI GTTAAC 1 cut(s) 882
HpaII CCGG 3 cut(s) 15, 31, 1149
HphI GGTGA 6 cut(s) 670, 692, 702, 981, 1106, 1184
Hpy166II GTNNAC 3 cut(s) 683, 882, 889
Hpy188I TCNGA 3 cut(s) 331, 925, 1117
Hpy188III TCNNGA 6 cut(s) 247, 269, 292, 611, 1067, 1202
Hpy8I GTNNAC 3 cut(s) 683, 882, 889
Hpy99I CGWCG 1 cut(s) 203
HpyAV CCTTC 3 cut(s) 619, 1084, 1199
HpyCH4III ACNGT 9 cut(s) 61, 323, 575, 742, 831, 845, 886, 981, 1276
HpyCH4IV ACGT 1 cut(s) 1060
HpyCH4V TGCA 1 cut(s) 746
HpyF10VI GCNNNNNNNGC 7 cut(s) 11, 194, 209, 218, 227, 359, 1165
HpyF3I CTNAG 4 cut(s) 354, 924, 1050, 1114
HpySE526I ACGT 1 cut(s) 1060
Hsp92I GRCGYC 1 cut(s) 809
Hsp92II CATG 9 cut(s) 14, 157, 424, 450, 510, 691, 726, 1032, 1070
KspAI GTTAAC 1 cut(s) 882
Kzo9I GATC 7 cut(s) 265, 279, 513, 628, 707, 1040, 1108
LguI GCTCTTC 1 cut(s) 135
LmnI GCTCC 2 cut(s) 286, 403
Lsp1109I GCAGC 2 cut(s) 221, 1171
LweI GCATC 3 cut(s) 861, 995, 1277
MaeI CTAG 3 cut(s) 611, 1143, 1202
MaeII ACGT 1 cut(s) 1060
MaeIII GTNAC 4 cut(s) 55, 845, 1094, 1258
MalI GATC 7 cut(s) 267, 281, 515, 630, 709, 1042, 1110
MboI GATC 7 cut(s) 265, 279, 513, 628, 707, 1040, 1108
MboII GAAGA 3 cut(s) 152, 827, 1145
MflI RGATCY 2 cut(s) 265, 628
MhlI GDGCHC 3 cut(s) 291, 355, 738
MluCI AATT 8 cut(s) 37, 345, 486, 520, 973, 1185, 1197, 1303
MlyI GAGTC 6 cut(s) 120, 129, 266, 302, 702, 1079
MmeI TCCRAC 1 cut(s) 50
MseI TTAA 7 cut(s) 182, 314, 485, 519, 648, 881, 1281
MslI CAYNNNNRTG 1 cut(s) 1104
MspI CCGG 3 cut(s) 15, 31, 1149
MspR9I CCNGG 6 cut(s) 16, 31, 460, 475, 502, 1149
MvaI CCWGG 3 cut(s) 460, 475, 502
MwoI GCNNNNNNNGC 7 cut(s) 11, 194, 209, 218, 227, 359, 1165
NciI CCSGG 3 cut(s) 16, 31, 1149
NcoI CCATGG 2 cut(s) 153, 420
NdeII GATC 7 cut(s) 265, 279, 513, 628, 707, 1040, 1108
NlaIII CATG 9 cut(s) 14, 157, 424, 450, 510, 691, 726, 1032, 1070
NlaIV GGNNCC 4 cut(s) 24, 384, 426, 630
NmuCI GTSAC 4 cut(s) 55, 845, 1094, 1258
NspI RCATGY 1 cut(s) 510
PaeI GCATGC 1 cut(s) 510
PaeR7I CTCGAG 1 cut(s) 290
PagI TCATGA 1 cut(s) 1066
PaqCI CACCTGC 1 cut(s) 1009
PciSI GCTCTTC 1 cut(s) 135
PfeI GAWTC 1 cut(s) 170
PkrI GCNGC 2 cut(s) 211, 1161
PleI GAGTC 6 cut(s) 120, 129, 266, 302, 701, 1078
PpsI GAGTC 6 cut(s) 120, 129, 266, 302, 701, 1078
PshBI ATTAAT 1 cut(s) 485
Psp124BI GAGCTC 1 cut(s) 291
Psp6I CCWGG 3 cut(s) 458, 473, 500
PspEI GGTNACC 1 cut(s) 1094
PspFI CCCAGC 1 cut(s) 19
PspGI CCWGG 3 cut(s) 458, 473, 500
PspN4I GGNNCC 4 cut(s) 24, 384, 426, 630
PspPI GGNCC 3 cut(s) 23, 33, 957
PstNI CAGNNNCTG 2 cut(s) 1024, 1230
PsuI RGATCY 2 cut(s) 265, 628
RsaI GTAC 4 cut(s) 492, 816, 983, 1223
RsaNI GTAC 4 cut(s) 491, 815, 982, 1222
RseI CAYNNNNRTG 1 cut(s) 1104
SacI GAGCTC 1 cut(s) 291
SapI GCTCTTC 1 cut(s) 135
SaqAI TTAA 7 cut(s) 182, 314, 485, 519, 648, 881, 1281
SatI GCNGC 2 cut(s) 210, 1160
Sau3AI GATC 7 cut(s) 265, 279, 513, 628, 707, 1040, 1108
Sau96I GGNCC 3 cut(s) 23, 33, 957
SchI GAGTC 6 cut(s) 120, 129, 266, 302, 702, 1079
ScrFI CCNGG 6 cut(s) 16, 31, 460, 475, 502, 1149
SduI GDGCHC 3 cut(s) 291, 355, 738
SfaNI GCATC 3 cut(s) 861, 995, 1277
SfcI CTRYAG 1 cut(s) 165
Sfr274I CTCGAG 1 cut(s) 290
SinI GGWCC 2 cut(s) 23, 33
SlaI CTCGAG 1 cut(s) 290
SmiMI CAYNNNNRTG 1 cut(s) 1104
SmlI CTYRAG 3 cut(s) 290, 302, 548
SmoI CTYRAG 3 cut(s) 290, 302, 548
SphI GCATGC 1 cut(s) 510
Sse9I AATT 8 cut(s) 37, 345, 486, 520, 973, 1185, 1197, 1303
SsiI CCGC 2 cut(s) 219, 1166
SspMI CTAG 3 cut(s) 611, 1143, 1202
SstI GAGCTC 1 cut(s) 291
StyD4I CCNGG 6 cut(s) 14, 29, 458, 473, 500, 1147
StyI CCWWGG 4 cut(s) 153, 261, 420, 654
TaaI ACNGT 9 cut(s) 61, 323, 575, 742, 831, 845, 886, 981, 1276
TaiI ACGT 1 cut(s) 1063
TaqI TCGA 6 cut(s) 138, 291, 343, 641, 1039, 1103
TasI AATT 8 cut(s) 37, 345, 486, 520, 973, 1185, 1197, 1303
TatI WGTACW 1 cut(s) 814
TfiI GAWTC 1 cut(s) 170
Tru1I TTAA 7 cut(s) 182, 314, 485, 519, 648, 881, 1281
Tru9I TTAA 7 cut(s) 182, 314, 485, 519, 648, 881, 1281
TscAI CASTG 6 cut(s) 232, 326, 578, 745, 909, 984
TseFI GTSAC 4 cut(s) 55, 845, 1094, 1258
TseI GCWGC 2 cut(s) 209, 1159
Tsp45I GTSAC 4 cut(s) 55, 845, 1094, 1258
TspDTI ATGAA 1 cut(s) 1066
TspGWI ACGGA 1 cut(s) 395
TspRI CASTG 6 cut(s) 232, 326, 578, 745, 909, 984
VpaK11BI GGWCC 2 cut(s) 23, 33
VspI ATTAAT 1 cut(s) 485
XapI RAATTY 2 cut(s) 345, 973
XbaI TCTAGA 2 cut(s) 610, 1201
XceI RCATGY 1 cut(s) 510
XhoI CTCGAG 1 cut(s) 290
XmiI GTMKAC 1 cut(s) 888
XspI CTAG 3 cut(s) 611, 1143, 1202
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.