Rmu_sc0001998.1_g000030

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001998.1
OG0006290
Physical Location & Seq
Forward (+)
133502 .. 135690
2189 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2106 bp
atgtcacttagatctctcacccttaaacccaaaaccctccactctttcttcaaccctcttctccgacccaccaaacccaacactcccttccgccctttctcttctctcacccaccaaaacccaccatctccacaattacctcacctggtcaacgaaatctcacgcattctcagcgaccacagaaaccctcaccacgacttggagctttccctcaactcttggtccacccaaatatcccctaacttggttgaacaggttctgaaacgctgcaaaaatcttgggttttcagcccacagattctttctctgggctaaaaaaattccgggttttcaacacagtggtgaaagccatcacattttgattgatatcttggggagtagtggtcagtttgctctgttgtgggattttctcattgaaatgagagagtccaagtgttgtgagattagcccagaattgttctgggttatttttagagtttacagtagagctaatttgcctcgtgatgcgattcgagcttttaatagaatggttgagtttggagtcaagcctagtgtgcatgatcttgatcagcttctctatacattatgtaaaagaaagcatgtcaagcatgcccaggagttttttgataaagttaagagtgggtttgagttgggtgctaaaacttatagcattttgatgaggggttggggagacatttgtgattcggataatgcacgcaagctgttcgatgaaatgactgaaagagggtgtttggtggatgtgcctgcatataatagttatttggaggctttgtgtaaaggtggcaatgtggatgaggcttataagatatttcgggcgatgggttcaaaaggagttgagcccgatgctggtacatactcgattttcattcgggcatattgtgaagcgaatgatattcattcggtttttagcgtgcttgataggatgaagagatataatcttttgcctaatgtgtattcttacaattgtatcatcaagaagctgtgtaagaatgaaaaggtggaagaggcgtaccaacttttggatgaaatgattgaaatgggagttaagccggatgaatggagttacaatgcaatccaagcttatcattgtgaccattgtgaagttaatcaggctcttaggttgttatctagaatggaaaaagataactgcatgccagatcggcatacctacaatatggtactaaagttgctgatcaggattggaagatttgatagggcaactgaagtgtgggagagtatggggaggagaggcttttatccttctgtttcaacatattctgtcatgattcatggtttgtgcaaaaagaaacaaaaactagaggaggcatgtaaatactttgagataatgattgatgaaggagttgagccagatgctggtacatactcgattttcgttcgggcatattgtgaagcgaatgatattcattcggtttttagcgtgcttgataggatgaagagatataatcttttgcctaatgtgtattcttacaattgtatcatcaagaagctgtgtaagaatgaaaaggtggaagaggcgtaccaacttttggatgaaatgattgaaatgggagttaagccggatgaatggagttacaatgcaatccaagcttatcattgtgagcattgtgaagttaatcaggctcttaggttgttatctagaatggaaaaagatagctgcatgccagatcggcatacttacaatatggtactaaagttgctgatcaggatcggaagatttgatagggcaactgaagtgtgggagagtatggggaagagaggcttttatccttctgtttcaacatattctgtcatgattcatggtttgtgcaaaaagaaacacaaactagaggaggcatgtaaatactttgagataatgattgatgaaggtataccaccatactcttctactgtggagatgttgagaaaccggcttttgggtttggggctacttgatgacattgaaatacttgctagtaagatggagcaaagtacttcttgctcgatacaagagtttgcaaaagtgctgagagggaacaaagcttctgtaagatcgagaagtgagtacactgacattgaaagtgactga

Protein Analysis

701

Amino Acids

81.3

Weight (kDa)

7.72

Isoelectric Point (pI)

41.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_long PF17177 237 - 365 2.6e-12 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 240 - 299 1.2e-07 Pentatricopeptide repeat domain
PPR_1 PF12854 248 - 280 2.8e-06 PPR repeat
PPR_2 PF13041 253 - 300 2.7e-14 PPR repeat family
PPR_3 PF13812 275 - 334 3.2e-10 Pentatricopeptide repeat domain
TPR_24 PF23276 278 - 353 2.7e-06 Fungal tetratrico peptide repeats
PPR_1 PF12854 283 - 316 1.3e-06 PPR repeat
PPR_2 PF13041 291 - 336 9e-12 PPR repeat family
PPR_3 PF13812 310 - 365 4.4e-07 Pentatricopeptide repeat domain
PPR_1 PF12854 319 - 350 2.1e-11 PPR repeat
PPR_2 PF13041 322 - 364 3.3e-14 PPR repeat family
PPR PF01535 325 - 355 1.8e-07 PPR repeat
PPR_3 PF13812 346 - 403 4.7e-06 Pentatricopeptide repeat domain
PPR_2 PF13041 361 - 404 2.9e-09 PPR repeat family
PPR_2 PF13041 396 - 441 1.2e-12 PPR repeat family
PPR_long PF17177 442 - 540 5.2e-10 Pentacotripeptide-repeat region of PRORP
PPR_2 PF13041 442 - 476 6.5e-07 PPR repeat family
PPR_3 PF13812 456 - 510 4.9e-08 Pentatricopeptide repeat domain
PPR_2 PF13041 473 - 512 2e-09 PPR repeat family
PPR_3 PF13812 486 - 541 4.9e-07 Pentatricopeptide repeat domain
PPR_1 PF12854 495 - 526 2.1e-11 PPR repeat
PPR_2 PF13041 498 - 540 3.3e-14 PPR repeat family
PPR PF01535 501 - 531 1.8e-07 PPR repeat
PPR_3 PF13812 522 - 579 4.7e-06 Pentatricopeptide repeat domain
PPR_2 PF13041 537 - 580 5e-09 PPR repeat family
PPR_2 PF13041 572 - 617 5.8e-13 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 822
AccB7I CCANNNNNTGG 4 cut(s) 199, 1039, 1394, 1567
AccI GTMKAC 1 cut(s) 1909
AciI CCGC 1 cut(s) 91
AclWI GGATC 1 cut(s) 1754
AcsI RAATTY 1 cut(s) 318
AcuI CTGAAG 2 cut(s) 1263, 1791
AfaI GTAC 8 cut(s) 871, 1031, 1200, 1399, 1559, 1728, 2011, 2084
AfiI CCNNNNNNNGG 7 cut(s) 199, 244, 866, 1039, 1394, 1567, 1954
AjnI CCWGG 2 cut(s) 144, 612
AjuI GAANNNNNNNTTGG 2 cut(s) 71, 103
AleI CACNNNNGTG 1 cut(s) 339
Alw26I GTCTC 1 cut(s) 684
AlwI GGATC 1 cut(s) 1754
AlwNI CAGNNNCTG 2 cut(s) 259, 1394
ApeKI GCWGC 2 cut(s) 267, 1695
ApoI RAATTY 1 cut(s) 318
Asp700I GAANNNNTTC 1 cut(s) 255
AspS9I GGNCC 1 cut(s) 222
AsuC2I CCSGG 1 cut(s) 324
AsuHPI GGTGA 5 cut(s) 10, 100, 134, 182, 353
AvaII GGWCC 1 cut(s) 222
BanII GRGCYC 1 cut(s) 861
BauI CACGAG 1 cut(s) 498
BbvI GCAGC 2 cut(s) 254, 1682
BccI CCATC 4 cut(s) 133, 357, 832, 1993
BcgI CGANNNNNNTGC 2 cut(s) 706, 740
BciT130I CCWGG 2 cut(s) 146, 614
BclI TGATCA 3 cut(s) 565, 1212, 1740
BcnI CCSGG 1 cut(s) 324
BcoDI GTCTC 1 cut(s) 684
BfaI CTAG 6 cut(s) 549, 1149, 1337, 1677, 1865, 1992
BglI GCCNNNNNGGC 2 cut(s) 1180, 1708
BglII AGATCT 1 cut(s) 11
BisI GCNGC 2 cut(s) 268, 1696
BlsI GCNGC 2 cut(s) 269, 1697
BmcAI AGTACT 1 cut(s) 2011
Bme1390I CCNGG 3 cut(s) 146, 324, 614
Bme18I GGWCC 1 cut(s) 222
BmgT120I GGNCC 1 cut(s) 222
BmrFI CCNGG 3 cut(s) 146, 324, 614
BmsI GCATC 3 cut(s) 493, 853, 1381
BpuMI CCSGG 1 cut(s) 324
BsaBI GATNNNNATC 3 cut(s) 365, 564, 1745
BsaJI CCNNGG 1 cut(s) 612
BsaXI ACNNNNNCTCC 4 cut(s) 367, 397, 776, 806
Bsc4I CCNNNNNNNGG 7 cut(s) 199, 244, 866, 1039, 1394, 1567, 1954
Bse118I RCCGGY 1 cut(s) 1947
Bse3DI GCAATG 1 cut(s) 811
Bse8I GATNNNNATC 3 cut(s) 365, 564, 1745
BseBI CCWGG 2 cut(s) 146, 614
BseDI CCNNGG 1 cut(s) 612
BseGI GGATG 8 cut(s) 763, 817, 948, 1048, 1078, 1476, 1576, 1606
BseJI GATNNNNATC 3 cut(s) 365, 564, 1745
BseLI CCNNNNNNNGG 7 cut(s) 199, 244, 866, 1039, 1394, 1567, 1954
BseMI GCAATG 1 cut(s) 811
BseMII CTCAG 2 cut(s) 184, 2036
BseRI GAGGAG 3 cut(s) 1279, 1355, 1883
BseXI GCAGC 2 cut(s) 254, 1682
BsiSI CCGG 4 cut(s) 323, 1070, 1598, 1948
BslI CCNNNNNNNGG 7 cut(s) 199, 244, 866, 1039, 1394, 1567, 1954
BsmAI GTCTC 1 cut(s) 684
BsmI GAATGC 1 cut(s) 165
Bsp1286I GDGCHC 1 cut(s) 861
Bsp143I GATC 9 cut(s) 11, 559, 565, 1177, 1212, 1705, 1740, 1746, 2069
BspACI CCGC 1 cut(s) 91
BspCNI CTCAG 2 cut(s) 183, 2037
BspHI TCATGA 2 cut(s) 1302, 1830
BspPI GGATC 1 cut(s) 1754
BsrDI GCAATG 1 cut(s) 811
BsrFI RCCGGY 1 cut(s) 1947
BssAI RCCGGY 1 cut(s) 1947
BssECI CCNNGG 1 cut(s) 612
BssMI GATC 9 cut(s) 11, 559, 565, 1177, 1212, 1705, 1740, 1746, 2069
BssNAI GTATAC 1 cut(s) 1910
BssSI CACGAG 1 cut(s) 498
Bst1107I GTATAC 1 cut(s) 1910
Bst2BI CACGAG 1 cut(s) 498
Bst2UI CCWGG 2 cut(s) 146, 614
Bst4CI ACNGT 3 cut(s) 338, 482, 1930
Bst6I CTCTTC 8 cut(s) 63, 106, 941, 1017, 1469, 1545, 1787, 1927
BstC8I GCNNGC 8 cut(s) 609, 715, 719, 765, 932, 1172, 1460, 1700
BstDEI CTNAG 5 cut(s) 8, 170, 1136, 1664, 2045
BstF5I GGATG 8 cut(s) 763, 817, 948, 1048, 1078, 1476, 1576, 1606
BstKTI GATC 9 cut(s) 14, 562, 568, 1180, 1215, 1708, 1743, 1749, 2072
BstMAI GTCTC 1 cut(s) 684
BstMBI GATC 9 cut(s) 11, 559, 565, 1177, 1212, 1705, 1740, 1746, 2069
BstMWI GCNNNNNNNGC 8 cut(s) 171, 512, 553, 604, 1097, 1180, 1625, 1708
BstNI CCWGG 2 cut(s) 146, 614
BstNSI RCATGY 6 cut(s) 602, 611, 1174, 1350, 1702, 1878
BstSCI CCNGG 3 cut(s) 144, 322, 612
BstV1I GCAGC 2 cut(s) 254, 1682
BstX2I RGATCY 1 cut(s) 11
BstYI RGATCY 1 cut(s) 11
BstZ17I GTATAC 1 cut(s) 1910
BtgZI GCGATG 1 cut(s) 851
BtsCI GGATG 8 cut(s) 763, 817, 948, 1048, 1078, 1476, 1576, 1606
BtsIMutI CAGTG 2 cut(s) 343, 2085
Cac8I GCNNGC 8 cut(s) 609, 715, 719, 765, 932, 1172, 1460, 1700
CaiI CAGNNNCTG 2 cut(s) 259, 1394
CciI TCATGA 2 cut(s) 1302, 1830
Cfr10I RCCGGY 1 cut(s) 1947
Cfr13I GGNCC 1 cut(s) 222
CsiI ACCWGGT 1 cut(s) 144
Csp6I GTAC 8 cut(s) 870, 1030, 1199, 1398, 1558, 1727, 2010, 2083
CviQI GTAC 8 cut(s) 870, 1030, 1199, 1398, 1558, 1727, 2010, 2083
DdeI CTNAG 5 cut(s) 8, 170, 1136, 1664, 2045
DpnI GATC 9 cut(s) 13, 561, 567, 1179, 1214, 1707, 1742, 1748, 2071
DpnII GATC 9 cut(s) 11, 559, 565, 1177, 1212, 1705, 1740, 1746, 2069
Eam1104I CTCTTC 8 cut(s) 63, 106, 941, 1017, 1469, 1545, 1787, 1927
EarI CTCTTC 8 cut(s) 63, 106, 941, 1017, 1469, 1545, 1787, 1927
EciI GGCGGA 1 cut(s) 80
Eco24I GRGCYC 1 cut(s) 861
Eco32I GATATC 1 cut(s) 367
Eco47I GGWCC 1 cut(s) 222
Eco57I CTGAAG 2 cut(s) 1263, 1791
EcoRII CCWGG 2 cut(s) 144, 612
EcoRV GATATC 1 cut(s) 367
EcoT38I GRGCYC 1 cut(s) 861
FalI AAGNNNNNCTT 4 cut(s) 1784, 1816, 1999, 2031
FbaI TGATCA 3 cut(s) 565, 1212, 1740
FblI GTMKAC 1 cut(s) 1909
Fnu4HI GCNGC 2 cut(s) 268, 1696
FokI GGATG 8 cut(s) 770, 824, 955, 1055, 1085, 1483, 1583, 1613
FriOI GRGCYC 1 cut(s) 861
Fsp4HI GCNGC 2 cut(s) 268, 1696
FspBI CTAG 6 cut(s) 549, 1149, 1337, 1677, 1865, 1992
GluI GCNGC 2 cut(s) 268, 1696
HapII CCGG 4 cut(s) 323, 1070, 1598, 1948
HincII GTYRAC 1 cut(s) 151
HindII GTYRAC 1 cut(s) 151
HindIII AAGCTT 3 cut(s) 1098, 1626, 2058
HinfI GANTC 7 cut(s) 297, 425, 508, 540, 701, 1306, 1834
HpaII CCGG 4 cut(s) 323, 1070, 1598, 1948
HphI GGTGA 5 cut(s) 10, 100, 134, 182, 353
Hpy166II GTNNAC 5 cut(s) 151, 225, 478, 1910, 2085
Hpy188I TCNGA 4 cut(s) 65, 261, 706, 1751
Hpy8I GTNNAC 5 cut(s) 151, 225, 478, 1910, 2085
HpyAV CCTTC 5 cut(s) 97, 1290, 1370, 1818, 1898
HpyCH4III ACNGT 3 cut(s) 338, 482, 1930
HpyF10VI GCNNNNNNNGC 8 cut(s) 171, 512, 553, 604, 1097, 1180, 1625, 1708
HpyF3I CTNAG 5 cut(s) 8, 170, 1136, 1664, 2045
Ksp22I TGATCA 3 cut(s) 565, 1212, 1740
Kzo9I GATC 9 cut(s) 11, 559, 565, 1177, 1212, 1705, 1740, 1746, 2069
LmnI GCTCC 2 cut(s) 202, 2002
Lsp1109I GCAGC 2 cut(s) 254, 1682
LweI GCATC 3 cut(s) 493, 853, 1381
MabI ACCWGGT 1 cut(s) 144
MaeI CTAG 6 cut(s) 549, 1149, 1337, 1677, 1865, 1992
MaeIII GTNAC 5 cut(s) 3, 1082, 1109, 1610, 2099
MalI GATC 9 cut(s) 13, 561, 567, 1179, 1214, 1707, 1742, 1748, 2071
MboI GATC 9 cut(s) 11, 559, 565, 1177, 1212, 1705, 1740, 1746, 2069
MfeI CAATTG 2 cut(s) 982, 1510
MflI RGATCY 1 cut(s) 11
MhlI GDGCHC 1 cut(s) 861
MluCI AATT 6 cut(s) 134, 318, 452, 490, 982, 1510
MlyI GAGTC 2 cut(s) 434, 549
MmeI TCCRAC 1 cut(s) 88
MroXI GAANNNNTTC 1 cut(s) 255
MseI TTAA 7 cut(s) 24, 519, 633, 1065, 1125, 1593, 1653
MslI CAYNNNNRTG 3 cut(s) 339, 416, 674
MspI CCGG 4 cut(s) 323, 1070, 1598, 1948
MspR9I CCNGG 3 cut(s) 146, 324, 614
MunI CAATTG 2 cut(s) 982, 1510
Mva1269I GAATGC 1 cut(s) 165
MvaI CCWGG 2 cut(s) 146, 614
MwoI GCNNNNNNNGC 8 cut(s) 171, 512, 553, 604, 1097, 1180, 1625, 1708
NciI CCSGG 1 cut(s) 324
NdeII GATC 9 cut(s) 11, 559, 565, 1177, 1212, 1705, 1740, 1746, 2069
NmuCI GTSAC 3 cut(s) 3, 1109, 2099
NspI RCATGY 6 cut(s) 602, 611, 1174, 1350, 1702, 1878
OliI CACNNNNGTG 1 cut(s) 339
PaeI GCATGC 3 cut(s) 611, 1174, 1702
PagI TCATGA 2 cut(s) 1302, 1830
PctI GAATGC 1 cut(s) 165
PdmI GAANNNNTTC 1 cut(s) 255
PfeI GAWTC 5 cut(s) 297, 508, 701, 1306, 1834
PflMI CCANNNNNTGG 4 cut(s) 199, 1039, 1394, 1567
PkrI GCNGC 2 cut(s) 269, 1697
PleI GAGTC 2 cut(s) 433, 548
PpsI GAGTC 2 cut(s) 433, 548
PsiI TTATAA 1 cut(s) 822
Psp6I CCWGG 2 cut(s) 144, 612
PspGI CCWGG 2 cut(s) 144, 612
PspPI GGNCC 1 cut(s) 222
PstNI CAGNNNCTG 2 cut(s) 259, 1394
PsuI RGATCY 1 cut(s) 11
RsaI GTAC 8 cut(s) 871, 1031, 1200, 1399, 1559, 1728, 2011, 2084
RsaNI GTAC 8 cut(s) 870, 1030, 1199, 1398, 1558, 1727, 2010, 2083
RseI CAYNNNNRTG 3 cut(s) 339, 416, 674
SaqAI TTAA 7 cut(s) 24, 519, 633, 1065, 1125, 1593, 1653
SatI GCNGC 2 cut(s) 268, 1696
Sau3AI GATC 9 cut(s) 11, 559, 565, 1177, 1212, 1705, 1740, 1746, 2069
Sau96I GGNCC 1 cut(s) 222
ScaI AGTACT 1 cut(s) 2011
SchI GAGTC 2 cut(s) 434, 549
ScrFI CCNGG 3 cut(s) 146, 324, 614
SduI GDGCHC 1 cut(s) 861
SexAI ACCWGGT 1 cut(s) 144
SfaNI GCATC 3 cut(s) 493, 853, 1381
SinI GGWCC 1 cut(s) 222
SmiMI CAYNNNNRTG 3 cut(s) 339, 416, 674
SphI GCATGC 3 cut(s) 611, 1174, 1702
Sse9I AATT 6 cut(s) 134, 318, 452, 490, 982, 1510
SsiI CCGC 1 cut(s) 91
SspMI CTAG 6 cut(s) 549, 1149, 1337, 1677, 1865, 1992
StyD4I CCNGG 3 cut(s) 144, 322, 612
TaaI ACNGT 3 cut(s) 338, 482, 1930
TaqI TCGA 6 cut(s) 511, 726, 878, 1406, 2021, 2072
TasI AATT 6 cut(s) 134, 318, 452, 490, 982, 1510
TatI WGTACW 2 cut(s) 2009, 2082
TfiI GAWTC 5 cut(s) 297, 508, 701, 1306, 1834
Tru1I TTAA 7 cut(s) 24, 519, 633, 1065, 1125, 1593, 1653
Tru9I TTAA 7 cut(s) 24, 519, 633, 1065, 1125, 1593, 1653
TscAI CASTG 2 cut(s) 343, 2092
TseFI GTSAC 3 cut(s) 3, 1109, 2099
TseI GCWGC 2 cut(s) 267, 1695
Tsp45I GTSAC 3 cut(s) 3, 1109, 2099
TspRI CASTG 2 cut(s) 343, 2092
Van91I CCANNNNNTGG 4 cut(s) 199, 1039, 1394, 1567
VpaK11BI GGWCC 1 cut(s) 222
XapI RAATTY 1 cut(s) 318
XbaI TCTAGA 2 cut(s) 1148, 1676
XceI RCATGY 6 cut(s) 602, 611, 1174, 1350, 1702, 1878
XcmI CCANNNNNNNNNTGG 1 cut(s) 456
XmiI GTMKAC 1 cut(s) 1909
XmnI GAANNNNTTC 1 cut(s) 255
XspI CTAG 6 cut(s) 549, 1149, 1337, 1677, 1865, 1992
ZrmI AGTACT 1 cut(s) 2011
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.