Rmu_sc0002105.1_g000001

Belongs to the nonaspanin (TM9SF) (TC 9.A.2) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002105.1
OG0015388
Physical Location & Seq
Forward (+)
5902 .. 13262
7361 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2154 bp
atgatcgtccgtagtcttgatcctgaaaagaatcatctacgtccaaaggatgatgatgaagacgtgctaaaggaaaaagtgtcatgcttaagtacaataggctcattattgtacttagctcaatgcacaagaccggacatctcatttgcaatgaacttgttagttagatataactttgagccaatgcgacgccattggattgatgtaaaagatatctttcggtacttgagatgtacgattgatatgggcttgttctatctctacagagagatgatgaattcagatccatcatacaccagaaacgccgccaacactggcctgcgtcctctatccccatcccaaaacgtgtgttttggaagatccccacccgcgccggcgtccgccatgttccccaccgttggatctctctcactcttcgccttcgcttgtcttctcctctcccccatagccttcgcctccgaggcagatcacaagtatcaaacagaagaagcagttaccctttgggtaaacaaagttggcccttataacaatccacaagaaacatacaattactacagtcttcctttctgtcagccatctgatcatgctgctcacaaatggggtggtcttggtgaagtcctgggtggaaatgaacttattgatagccagattgacataaagttcggaaaaaatgtggatagggctaccatttgtcaacttgaacttgatgatcataaggttaaacaattcaaggatgcaattgagagtagctactggtttgagtttttcatggatgatctgcctttatgggggtttgttggtgagttgcaccaggataagaacagtgaaaatggcaaacatgttctttacacgcataaaagtattattattaagtacaataaagatcagataattcatgtcaatctcactcaagatagtccaaagcctctggaagcgggaaaaaaattggatctgacttactctgtcaaatggatttcaaccactatcacctttgcacgtcgatttgatgtttatttggactacccatttttcgagcaccaaatccattggttctccattttcaattccttcatgatggttatattcctcactggtctggtgtcaatgatactgatgcgcactcttaggaatgactatgcaaaatatgctcgggaagatgatgacctagagacactggaaagagatgttagtgaagagtctggctggaaacttgtgcatggggatgtttttcgacctcctcggagtttggttattctttcagctgtagttggcacgggagcacagttggcgttgctcgttctcctcgtcattttactggcaattgttgggatgttgtatgtcgggagaggtgcaattgttacaactttcatagtgtgttacgctcttacatctttcatttctggttatgtaagtggtgggatgtattcacggcatgggggtaaaaactggataaagtcgatgattctcacagcatctctatttccatttatgtgcttcggaattgggtttgtcttgaatacaattgctatattctacgggtctctagcagctataccattcggcactatggtagttgtctttgtcatttgggctttcatctcgtttcctctagcgcttcttggtacagttgttggaagaaactggagtggtgctcctaataatccatgtcgtgttaaaactattcctcgtccgattcctgagaaaaaatggtatttgacaccatccgtggtgtcgatgatgggaggacttttaccttttggaagcatattcatcgaaatgtactttgtcttcacgtccttctggaattacaaggtgtactatgtctatggttttatgcttcttgtgtttctgattctcatcattgttactgtatgcgtgacgattgtggggacttacttcttgcttaatgctgagaattaccactggcaatggacgtctttcttttctgctgcatcaacagctgtgtacgtgtacttgtattcaatatattactactatgcaaagacgaagatgtcaggtttcttccagacgagcttctattttggatacactctgatgttttgcctcggcttaggaatcctctgtggagctgttggatatttgggttcaaatttgtttgtgaggaggatctacagaaacatcaaatgcgactag

Protein Analysis

717

Amino Acids

81.8

Weight (kDa)

8.04

Isoelectric Point (pI)

36.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EMP70 PF02990 181 - 674 1.2e-180 Endomembrane protein 70
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015388)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G10950
fragaria_vesca FvH4_5g28830
malus_domestica MD08G1236600.v1.1 MD15G1428600.v1.1
prunus_persica Prupe.1G573400_v2.0.a1 Prupe.1G573400_v2.0.a1
pyrus_communis pycom08g20490 pycom15g37960
rosa_chinensis RchiOBHm_Chr7g0221061
rosa_laevigata RLG00000002178
rosa_multiflora Rmu_sc0002105.1_g000001 Rmu_sc0012763.1_g000003
rosa_roxburghii Rroxscaffold_3G00238770
rosa_rugosa Rorug07G0200400
rosa_samantha Rh7AG339800
rosa_wichuraiana Rw7G028910

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 525
AasI GACNNNNNNGTC 1 cut(s) 2011
AatII GACGTC 1 cut(s) 1937
Acc16I TGCGCA 1 cut(s) 1118
AccII CGCG 1 cut(s) 371
AciI CCGC 4 cut(s) 306, 369, 381, 935
AclWI GGATC 6 cut(s) 14, 278, 354, 409, 957, 2135
AcsI RAATTY 2 cut(s) 277, 2110
AcyI GRCGYC 3 cut(s) 190, 377, 1934
AfeI AGCGCT 1 cut(s) 1614
AfiI CCNNNNNNNGG 2 cut(s) 398, 788
AflII CTTAAG 1 cut(s) 88
AflIII ACRYGT 3 cut(s) 345, 838, 1968
AgsI TTSAA 7 cut(s) 701, 730, 978, 1063, 1516, 1983, 2109
AjiI CACGTC 3 cut(s) 64, 998, 1794
AjnI CCWGG 2 cut(s) 618, 810
AluBI AGCT 7 cut(s) 119, 750, 1262, 1550, 1961, 2034, 2090
AluI AGCT 7 cut(s) 119, 750, 1262, 1550, 1961, 2034, 2090
Alw21I GWGCWC 3 cut(s) 1038, 1282, 1654
Alw26I GTCTC 2 cut(s) 1163, 1545
AlwI GGATC 6 cut(s) 14, 278, 354, 409, 957, 2135
Ama87I CYCGRG 1 cut(s) 1149
Aor51HI AGCGCT 1 cut(s) 1614
AoxI GGCC 2 cut(s) 316, 517
ApeKI GCWGC 3 cut(s) 587, 1547, 1949
ApoI RAATTY 2 cut(s) 277, 2110
ArsI GACNNNNNNTTYG 4 cut(s) 128, 160, 644, 676
AspLEI GCGC 3 cut(s) 373, 1119, 1615
AspS9I GGNCC 1 cut(s) 518
AsuHPI GGTGA 3 cut(s) 623, 812, 979
AvaI CYCGRG 1 cut(s) 1149
BaeI ACNNNNGTAYC 2 cut(s) 1614, 1647
BarI GAAGNNNNNNTAC 2 cut(s) 1772, 1804
BbsI GAAGAC 4 cut(s) 66, 422, 551, 1780
Bbv12I GWGCWC 3 cut(s) 1038, 1282, 1654
BbvI GCAGC 3 cut(s) 574, 1559, 1936
BccI CCATC 6 cut(s) 295, 343, 583, 1069, 1729, 1732
BceAI ACGGC 1 cut(s) 1445
BcgI CGANNNNNNTGC 2 cut(s) 1753, 1787
BciT130I CCWGG 2 cut(s) 620, 812
BciVI GTATCC 1 cut(s) 2039
BclI TGATCA 2 cut(s) 580, 709
BcoDI GTCTC 2 cut(s) 1163, 1545
BfaI CTAG 4 cut(s) 1166, 1544, 1610, 2152
BfmI CTRYAG 4 cut(s) 262, 553, 1263, 2131
BfoI RGCGCY 1 cut(s) 1616
BfrI CTTAAG 1 cut(s) 88
BfuI GTATCC 1 cut(s) 2039
BglI GCCNNNNNGGC 1 cut(s) 461
BisI GCNGC 4 cut(s) 306, 588, 1548, 1950
BlsI GCNGC 4 cut(s) 307, 589, 1549, 1951
Bme1390I CCNGG 2 cut(s) 620, 812
BmeT110I CYCGRG 1 cut(s) 1149
BmgBI CACGTC 3 cut(s) 64, 998, 1794
BmgT120I GGNCC 1 cut(s) 518
BmrFI CCNGG 2 cut(s) 620, 812
BmsI GCATC 4 cut(s) 724, 1104, 1481, 1961
BpiI GAAGAC 4 cut(s) 66, 422, 551, 1780
BplI GAGNNNNNCTC 2 cut(s) 1636, 1668
BpmI CTGGAG 1 cut(s) 1663
Bpu10I CCTNAGC 1 cut(s) 2071
BpuEI CTTGAG 2 cut(s) 247, 894
BsaAI YACGTR 1 cut(s) 1969
BsaBI GATNNNNATC 1 cut(s) 1856
BsaHI GRCGYC 3 cut(s) 190, 377, 1934
BsaI GGTCTC 1 cut(s) 1545
BsaJI CCNNGG 5 cut(s) 459, 619, 1238, 1725, 2065
BsaWI WCCGGW 1 cut(s) 133
Bsc4I CCNNNNNNNGG 2 cut(s) 398, 788
Bse118I RCCGGY 1 cut(s) 373
Bse1I ACTGG 8 cut(s) 319, 758, 1096, 1179, 1320, 1451, 1646, 1928
Bse3DI GCAATG 2 cut(s) 156, 1934
Bse8I GATNNNNATC 1 cut(s) 1856
BseBI CCWGG 2 cut(s) 620, 812
BseDI CCNNGG 5 cut(s) 459, 619, 1238, 1725, 2065
BseGI GGATG 8 cut(s) 55, 335, 739, 778, 1228, 1335, 1425, 1721
BseJI GATNNNNATC 1 cut(s) 1856
BseLI CCNNNNNNNGG 2 cut(s) 398, 788
BseMI GCAATG 2 cut(s) 156, 1934
BseMII CTCAG 2 cut(s) 1689, 1902
BseNI ACTGG 8 cut(s) 319, 758, 1096, 1179, 1320, 1451, 1646, 1928
BseRI GAGGAG 4 cut(s) 425, 1227, 1292, 2137
BseXI GCAGC 3 cut(s) 574, 1559, 1936
Bsh1236I CGCG 1 cut(s) 371
BshFI GGCC 2 cut(s) 318, 519
BsiHKAI GWGCWC 3 cut(s) 1038, 1282, 1654
BsiHKCI CYCGRG 1 cut(s) 1149
BsiSI CCGG 2 cut(s) 134, 374
BslFI GGGAC 1 cut(s) 1903
BslI CCNNNNNNNGG 2 cut(s) 398, 788
BsmAI GTCTC 2 cut(s) 1163, 1545
BsmFI GGGAC 1 cut(s) 1903
BsnI GGCC 2 cut(s) 318, 519
Bso31I GGTCTC 1 cut(s) 1545
BsoBI CYCGRG 1 cut(s) 1149
Bsp1286I GDGCHC 3 cut(s) 1038, 1282, 1654
BspACI CCGC 4 cut(s) 306, 369, 381, 935
BspANI GGCC 2 cut(s) 318, 519
BspCNI CTCAG 2 cut(s) 1690, 1903
BspFNI CGCG 1 cut(s) 371
BspHI TCATGA 1 cut(s) 1071
BspPI GGATC 6 cut(s) 14, 278, 354, 409, 957, 2135
BspTI CTTAAG 1 cut(s) 88
BspTNI GGTCTC 1 cut(s) 1545
BsrDI GCAATG 2 cut(s) 156, 1934
BsrFI RCCGGY 1 cut(s) 373
BsrI ACTGG 8 cut(s) 319, 758, 1096, 1179, 1320, 1451, 1646, 1928
BssAI RCCGGY 1 cut(s) 373
BssECI CCNNGG 5 cut(s) 459, 619, 1238, 1725, 2065
BssNI GRCGYC 3 cut(s) 190, 377, 1934
Bst2UI CCWGG 2 cut(s) 620, 812
Bst4CI ACNGT 6 cut(s) 397, 557, 824, 1284, 1627, 1870
Bst6I CTCTTC 2 cut(s) 419, 1188
BstACI GRCGYC 3 cut(s) 190, 377, 1934
BstAFI CTTAAG 1 cut(s) 88
BstAPI GCANNNNNTGC 1 cut(s) 1145
BstBAI YACGTR 1 cut(s) 1969
BstC8I GCNNGC 2 cut(s) 320, 375
BstDEI CTNAG 5 cut(s) 115, 1124, 1698, 1911, 2071
BstDSI CCRYGG 1 cut(s) 1725
BstF5I GGATG 8 cut(s) 55, 335, 739, 778, 1228, 1335, 1425, 1721
BstFNI CGCG 1 cut(s) 371
BstH2I RGCGCY 1 cut(s) 1616
BstHHI GCGC 3 cut(s) 373, 1119, 1615
BstMAI GTCTC 2 cut(s) 1163, 1545
BstMWI GCNNNNNNNGC 4 cut(s) 461, 1145, 1286, 1958
BstNI CCWGG 2 cut(s) 620, 812
BstNSI RCATGY 1 cut(s) 842
BstSCI CCNGG 2 cut(s) 618, 810
BstSFI CTRYAG 4 cut(s) 262, 553, 1263, 2131
BstUI CGCG 1 cut(s) 371
BstV1I GCAGC 3 cut(s) 574, 1559, 1936
BstV2I GAAGAC 4 cut(s) 66, 422, 551, 1780
BstX2I RGATCY 5 cut(s) 283, 359, 401, 949, 2127
BstYI RGATCY 5 cut(s) 283, 359, 401, 949, 2127
BsuI GTATCC 1 cut(s) 2039
BsuRI GGCC 2 cut(s) 318, 519
BtgI CCRYGG 1 cut(s) 1725
BtrI CACGTC 3 cut(s) 64, 998, 1794
BtsCI GGATG 8 cut(s) 55, 335, 739, 778, 1228, 1335, 1425, 1721
BtsIMutI CAGTG 5 cut(s) 312, 829, 1089, 1172, 1921
Cac8I GCNNGC 2 cut(s) 320, 375
CciI TCATGA 1 cut(s) 1071
CfoI GCGC 3 cut(s) 373, 1119, 1615
Cfr10I RCCGGY 1 cut(s) 373
Cfr13I GGNCC 1 cut(s) 518
CseI GACGC 3 cut(s) 198, 311, 366
CspCI CAANNNNNGTGG 2 cut(s) 583, 618
DdeI CTNAG 5 cut(s) 115, 1124, 1698, 1911, 2071
DrdI GACNNNNNNGTC 1 cut(s) 2011
DseDI GACNNNNNNGTC 1 cut(s) 2011
Eam1104I CTCTTC 2 cut(s) 419, 1188
EarI CTCTTC 2 cut(s) 419, 1188
EciI GGCGGA 1 cut(s) 370
Eco31I GGTCTC 1 cut(s) 1545
Eco32I GATATC 1 cut(s) 214
Eco47III AGCGCT 1 cut(s) 1614
Eco88I CYCGRG 1 cut(s) 1149
EcoRI GAATTC 1 cut(s) 277
EcoRII CCWGG 2 cut(s) 618, 810
EcoRV GATATC 1 cut(s) 214
FaqI GGGAC 1 cut(s) 1903
FauI CCCGC 2 cut(s) 376, 928
FbaI TGATCA 2 cut(s) 580, 709
Fnu4HI GCNGC 4 cut(s) 306, 588, 1548, 1950
FokI GGATG 8 cut(s) 62, 322, 746, 785, 1235, 1342, 1432, 1708
Fsp4HI GCNGC 4 cut(s) 306, 588, 1548, 1950
FspAI RTGCGCAY 1 cut(s) 1118
FspBI CTAG 4 cut(s) 1166, 1544, 1610, 2152
FspI TGCGCA 1 cut(s) 1118
GlaI GCGC 3 cut(s) 372, 1118, 1614
GluI GCNGC 4 cut(s) 306, 588, 1548, 1950
GsuI CTGGAG 1 cut(s) 1663
HaeII RGCGCY 1 cut(s) 1616
HaeIII GGCC 2 cut(s) 318, 519
HapII CCGG 2 cut(s) 134, 374
HgaI GACGC 3 cut(s) 198, 311, 366
HhaI GCGC 3 cut(s) 373, 1119, 1615
Hin1I GRCGYC 3 cut(s) 190, 377, 1934
Hin6I GCGC 3 cut(s) 371, 1117, 1613
HinP1I GCGC 3 cut(s) 371, 1117, 1613
HincII GTYRAC 1 cut(s) 695
HindII GTYRAC 1 cut(s) 695
HinfI GANTC 6 cut(s) 31, 1196, 1462, 1693, 1852, 2076
HpaII CCGG 2 cut(s) 134, 374
HphI GGTGA 3 cut(s) 623, 812, 979
Hpy166II GTNNAC 5 cut(s) 508, 695, 1816, 1966, 1972
Hpy8I GTNNAC 5 cut(s) 508, 695, 1816, 1966, 1972
Hpy99I CGWCG 2 cut(s) 192, 1002
HpyAV CCTTC 4 cut(s) 430, 460, 1078, 1807
HpyCH4III ACNGT 6 cut(s) 397, 557, 824, 1284, 1627, 1870
HpyCH4IV ACGT 7 cut(s) 40, 63, 345, 997, 1793, 1934, 1968
HpyF10VI GCNNNNNNNGC 4 cut(s) 461, 1145, 1286, 1958
HpyF3I CTNAG 5 cut(s) 115, 1124, 1698, 1911, 2071
HpySE526I ACGT 7 cut(s) 40, 63, 345, 997, 1793, 1934, 1968
Hsp92I GRCGYC 3 cut(s) 190, 377, 1934
HspAI GCGC 3 cut(s) 371, 1117, 1613
KroI GCCGGC 1 cut(s) 373
KroNI GCCGGC 1 cut(s) 375
Ksp22I TGATCA 2 cut(s) 580, 709
LmnI GCTCC 3 cut(s) 1277, 1657, 2087
Lsp1109I GCAGC 3 cut(s) 574, 1559, 1936
LweI GCATC 4 cut(s) 724, 1104, 1481, 1961
MaeI CTAG 4 cut(s) 1166, 1544, 1610, 2152
MaeII ACGT 7 cut(s) 40, 63, 345, 997, 1793, 1934, 1968
MaeIII GTNAC 5 cut(s) 493, 1357, 1376, 1864, 1876
MfeI CAATTG 4 cut(s) 738, 1320, 1353, 1521
MflI RGATCY 5 cut(s) 283, 359, 401, 949, 2127
MhlI GDGCHC 3 cut(s) 1038, 1282, 1654
MlyI GAGTC 1 cut(s) 1205
MmeI TCCRAC 3 cut(s) 379, 1612, 2074
MreI CGCCGGCG 1 cut(s) 373
MroNI GCCGGC 1 cut(s) 373
MseI TTAA 5 cut(s) 89, 720, 870, 1674, 1905
MslI CAYNNNNRTG 4 cut(s) 1221, 1489, 1775, 2054
MspA1I CMGCKG 2 cut(s) 1262, 1961
MspCI CTTAAG 1 cut(s) 88
MspI CCGG 2 cut(s) 134, 374
MspR9I CCNGG 2 cut(s) 620, 812
MunI CAATTG 4 cut(s) 738, 1320, 1353, 1521
MvaI CCWGG 2 cut(s) 620, 812
MvnI CGCG 1 cut(s) 371
MwoI GCNNNNNNNGC 4 cut(s) 461, 1145, 1286, 1958
NaeI GCCGGC 1 cut(s) 375
NgoMIV GCCGGC 1 cut(s) 373
NmeAIII GCCGAG 1 cut(s) 2046
NmuCI GTSAC 1 cut(s) 1876
NsbI TGCGCA 1 cut(s) 1118
NspI RCATGY 1 cut(s) 842
PagI TCATGA 1 cut(s) 1071
PciI ACATGT 1 cut(s) 838
PcsI WCGNNNNNNNCGW 1 cut(s) 1302
PdiI GCCGGC 1 cut(s) 375
PfeI GAWTC 5 cut(s) 31, 1462, 1693, 1852, 2076
PkrI GCNGC 4 cut(s) 307, 589, 1549, 1951
PleI GAGTC 1 cut(s) 1204
PpsI GAGTC 1 cut(s) 1204
Ppu21I YACGTR 1 cut(s) 1969
PscI ACATGT 1 cut(s) 838
PsiI TTATAA 1 cut(s) 525
Psp6I CCWGG 2 cut(s) 618, 810
PspGI CCWGG 2 cut(s) 618, 810
PspPI GGNCC 1 cut(s) 518
PsuI RGATCY 5 cut(s) 283, 359, 401, 949, 2127
PvuII CAGCTG 2 cut(s) 1262, 1961
RseI CAYNNNNRTG 4 cut(s) 1221, 1489, 1775, 2054
SaqAI TTAA 5 cut(s) 89, 720, 870, 1674, 1905
SatI GCNGC 4 cut(s) 306, 588, 1548, 1950
Sau96I GGNCC 1 cut(s) 518
SchI GAGTC 1 cut(s) 1205
ScrFI CCNGG 2 cut(s) 620, 812
SduI GDGCHC 3 cut(s) 1038, 1282, 1654
SfaNI GCATC 4 cut(s) 724, 1104, 1481, 1961
SfcI CTRYAG 4 cut(s) 262, 553, 1263, 2131
SgrAI CRCCGGYG 1 cut(s) 373
SmiMI CAYNNNNRTG 4 cut(s) 1221, 1489, 1775, 2054
SmlI CTYRAG 3 cut(s) 88, 226, 909
SmoI CTYRAG 3 cut(s) 88, 226, 909
SsiI CCGC 4 cut(s) 306, 369, 381, 935
SspMI CTAG 4 cut(s) 1166, 1544, 1610, 2152
StyD4I CCNGG 2 cut(s) 618, 810
TaaI ACNGT 6 cut(s) 397, 557, 824, 1284, 1627, 1870
TaiI ACGT 7 cut(s) 43, 66, 348, 1000, 1796, 1937, 1971
TaqI TCGA 6 cut(s) 1000, 1032, 1231, 1457, 1733, 1773
TatI WGTACW 6 cut(s) 92, 111, 873, 1779, 1815, 1971
TauI GCSGC 1 cut(s) 308
TfiI GAWTC 5 cut(s) 31, 1462, 1693, 1852, 2076
Tru1I TTAA 5 cut(s) 89, 720, 870, 1674, 1905
Tru9I TTAA 5 cut(s) 89, 720, 870, 1674, 1905
TscAI CASTG 5 cut(s) 319, 829, 1096, 1179, 1928
TseFI GTSAC 1 cut(s) 1876
TseI GCWGC 3 cut(s) 587, 1547, 1949
Tsp45I GTSAC 1 cut(s) 1876
TspGWI ACGGA 1 cut(s) 1714
TspRI CASTG 5 cut(s) 319, 829, 1096, 1179, 1928
Vha464I CTTAAG 1 cut(s) 88
XapI RAATTY 2 cut(s) 277, 2110
XceI RCATGY 1 cut(s) 842
XspI CTAG 4 cut(s) 1166, 1544, 1610, 2152
ZraI GACGTC 1 cut(s) 1935
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.