Rmu_sc0002251.1_g000006

source UniProtKB

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002251.1
OG0019052
Physical Location & Seq
Reverse (-)
20775 .. 24996
4222 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1287 bp
atggaacatcacattgccaaggaaagagctgcttgggaggataggtttaagaagttggaagaaaaggttgcgaggaaagaggttgacaatgattcattgaaaacgttgacaccgttgaatgatattggttctggacaaggcagttgttcccggcaaaatgacaagggtatgaaggttggtcaaactgagcttgctagaaatgtcaagaagaagttaatcttgaacaatgcttcacaagtggatgttgaagaacctctcatcaagactaaattctgggaagaagttctggttatgaccaatatggtcaagttggagtctaagctagcaataaattcattggataatgtagttgcttttggaaccatcgttgaagttgatattgaggccagcaaacaaacaatccatggtgttccaatgggagaggaaaatgtgcgcgtgtcagtcaccaaagctattgttgaagatgctttgctaccatttcctatcaaagatgagattgtcaaaatacgcgatgcgattggcacatgcgttgcttggcctaaaaacttgattataccccccacagttgagcaaaaggaaggagtaaagcgtaaaattgtgaagaagagaaatagagacatatatgatgatcatgatcatgatcatgatgaccttcaaaacctgccagccaagttgcctttacccctaaagatgttgtgccaatgggccaacacattctcaaaggatgggttcaccatccacaccactttgggagaagagcttttaggtcatccaaagaaggtagctatctttaggagggatgtgtatgccatgaccaacataaaggagatattgaacggctcattagtgatgtatatgaggtttgttttgaagaaaacaaagatgctggaaatgatcgccttcatagaccctgatcacacttgtgcacttgcatgtggaaatctaacagaacgttctctagctgttcggagcataaggtcgccggagttgaacaacgcggcggtatcgaagacgccgatcagatcagatctacttcaccttcatccttctcgccttgcgctgcgggatcatgagatagccgaattggtgttagaggcagtggatttaaggacctcgatgatccattttccggcgacgttatcctcatgcgcggagatgatgagaatctactccggctcgtctccgttcgggtccgcttcgactgagaaaggtaatggagatggaatcggcgacagaatcggcgatggcgttgaggaagggtgccgccggagccgagcgagatggtga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_C48 Transposase_24

Protein Analysis

428

Amino Acids

47.97

Weight (kDa)

7.65

Isoelectric Point (pI)

43.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF8039 PF26133 114 - 185 1.1e-11 Domain of unknown function (DUF8039)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019052)

Species Orthologous Gene IDs
rosa_laevigata RLG00000005583 RLG00000026519 RLG00000030610 RLG00000034821 RLG00000036644
rosa_multiflora Rmu_sc0002251.1_g000006
rosa_samantha Rh2AG669900 Rh2DG493100 Rh3DG269500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 669
AccB1I GGYRCC 1 cut(s) 1260
AccII CGCG 4 cut(s) 435, 510, 998, 1151
AciI CCGC 6 cut(s) 998, 1001, 1063, 1151, 1194, 1264
AclI AACGTT 2 cut(s) 104, 952
AclWI GGATC 2 cut(s) 1074, 1114
AcsI RAATTY 2 cut(s) 269, 331
AcyI GRCGYC 1 cut(s) 1013
AfiI CCNNNNNNNGG 1 cut(s) 1129
AleI CACNNNNGTG 1 cut(s) 921
AluBI AGCT 7 cut(s) 29, 190, 322, 452, 760, 785, 962
AluI AGCT 7 cut(s) 29, 190, 322, 452, 760, 785, 962
Alw21I GWGCWC 1 cut(s) 928
Alw26I GTCTC 2 cut(s) 609, 1185
Alw44I GTGCAC 1 cut(s) 924
AlwI GGATC 2 cut(s) 1074, 1114
AoxI GGCC 3 cut(s) 384, 536, 705
ApaLI GTGCAC 1 cut(s) 924
ApeKI GCWGC 2 cut(s) 29, 1060
ApoI RAATTY 2 cut(s) 269, 331
Asp700I GAANNNNTTC 1 cut(s) 282
AspLEI GCGC 3 cut(s) 435, 1060, 1151
AspS9I GGNCC 3 cut(s) 705, 1110, 1191
AsuC2I CCSGG 1 cut(s) 151
AsuHPI GGTGA 3 cut(s) 436, 724, 1028
AsuNHI GCTAGC 1 cut(s) 322
AvaII GGWCC 2 cut(s) 1110, 1191
BaeGI GKGCMC 1 cut(s) 928
BanI GGYRCC 1 cut(s) 1260
BbsI GAAGAC 1 cut(s) 1016
Bbv12I GWGCWC 1 cut(s) 928
BbvI GCAGC 2 cut(s) 16, 1047
BccI CCATC 6 cut(s) 371, 719, 743, 1214, 1238, 1275
BceAI ACGGC 1 cut(s) 853
BclI TGATCA 4 cut(s) 628, 634, 640, 913
BcnI CCSGG 1 cut(s) 151
BcoDI GTCTC 2 cut(s) 609, 1185
BfaI CTAG 3 cut(s) 195, 323, 959
BfuAI ACCTGC 1 cut(s) 669
BglII AGATCT 1 cut(s) 1027
BisI GCNGC 4 cut(s) 30, 999, 1061, 1264
BlsI GCNGC 4 cut(s) 31, 1000, 1062, 1265
Bme1390I CCNGG 1 cut(s) 151
Bme18I GGWCC 2 cut(s) 1110, 1191
BmgT120I GGNCC 3 cut(s) 705, 1110, 1191
BmiI GGNNCC 4 cut(s) 361, 1192, 1262, 1271
BmrFI CCNGG 1 cut(s) 151
BmsI GCATC 3 cut(s) 454, 502, 873
BmtI GCTAGC 1 cut(s) 326
BpiI GAAGAC 1 cut(s) 1016
BpuMI CCSGG 1 cut(s) 151
BsaBI GATNNNNATC 3 cut(s) 633, 639, 1163
BsaHI GRCGYC 1 cut(s) 1013
BsaJI CCNNGG 2 cut(s) 18, 403
BsaXI ACNNNNNCTCC 2 cut(s) 29, 59
Bsc4I CCNNNNNNNGG 1 cut(s) 1129
Bse3DI GCAATG 1 cut(s) 12
Bse8I GATNNNNATC 3 cut(s) 633, 639, 1163
BseDI CCNNGG 2 cut(s) 18, 403
BseGI GGATG 6 cut(s) 247, 730, 735, 769, 805, 1042
BseJI GATNNNNATC 3 cut(s) 633, 639, 1163
BseLI CCNNNNNNNGG 1 cut(s) 1129
BseMI GCAATG 1 cut(s) 12
BseMII CTCAG 2 cut(s) 177, 1194
BseSI GKGCMC 1 cut(s) 928
BseXI GCAGC 2 cut(s) 16, 1047
Bsh1236I CGCG 4 cut(s) 435, 510, 998, 1151
BshFI GGCC 3 cut(s) 386, 538, 707
BshNI GGYRCC 1 cut(s) 1260
BsiHKAI GWGCWC 1 cut(s) 928
BsiSI CCGG 5 cut(s) 151, 983, 1130, 1173, 1267
BslI CCNNNNNNNGG 1 cut(s) 1129
BsmAI GTCTC 2 cut(s) 609, 1185
BsmBI CGTCTC 1 cut(s) 1185
BsnI GGCC 3 cut(s) 386, 538, 707
Bsp1286I GDGCHC 1 cut(s) 928
Bsp19I CCATGG 1 cut(s) 403
BspACI CCGC 6 cut(s) 998, 1001, 1063, 1151, 1194, 1264
BspANI GGCC 3 cut(s) 386, 538, 707
BspCNI CTCAG 2 cut(s) 178, 1195
BspFNI CGCG 4 cut(s) 435, 510, 998, 1151
BspHI TCATGA 4 cut(s) 631, 637, 643, 1069
BspLI GGNNCC 4 cut(s) 361, 1192, 1262, 1271
BspMI ACCTGC 1 cut(s) 669
BspOI GCTAGC 1 cut(s) 326
BspPI GGATC 2 cut(s) 1074, 1114
BspQI GCTCTTC 1 cut(s) 750
BspT107I GGYRCC 1 cut(s) 1260
BsrDI GCAATG 1 cut(s) 12
BssECI CCNNGG 2 cut(s) 18, 403
BssNI GRCGYC 1 cut(s) 1013
BssT1I CCWWGG 2 cut(s) 18, 403
Bst4CI ACNGT 2 cut(s) 114, 565
Bst6I CTCTTC 2 cut(s) 599, 750
BstACI GRCGYC 1 cut(s) 1013
BstC8I GCNNGC 4 cut(s) 192, 324, 388, 666
BstDEI CTNAG 3 cut(s) 186, 318, 1203
BstDSI CCRYGG 1 cut(s) 403
BstF5I GGATG 6 cut(s) 247, 730, 735, 769, 805, 1042
BstFNI CGCG 4 cut(s) 435, 510, 998, 1151
BstHHI GCGC 3 cut(s) 435, 1060, 1151
BstMAI GTCTC 2 cut(s) 609, 1185
BstMWI GCNNNNNNNGC 1 cut(s) 1269
BstNSI RCATGY 2 cut(s) 528, 936
BstSCI CCNGG 1 cut(s) 149
BstSLI GKGCMC 1 cut(s) 928
BstUI CGCG 4 cut(s) 435, 510, 998, 1151
BstV1I GCAGC 2 cut(s) 16, 1047
BstV2I GAAGAC 1 cut(s) 1016
BstX2I RGATCY 1 cut(s) 1027
BstYI RGATCY 1 cut(s) 1027
BsuRI GGCC 3 cut(s) 386, 538, 707
BtgI CCRYGG 1 cut(s) 403
BtgZI GCGATG 2 cut(s) 525, 1257
BtsCI GGATG 6 cut(s) 247, 730, 735, 769, 805, 1042
BtsI GCAGTG 1 cut(s) 1104
BtsIMutI CAGTG 1 cut(s) 1104
BveI ACCTGC 1 cut(s) 669
Cac8I GCNNGC 4 cut(s) 192, 324, 388, 666
CciI TCATGA 4 cut(s) 631, 637, 643, 1069
CfoI GCGC 3 cut(s) 435, 1060, 1151
Cfr13I GGNCC 3 cut(s) 705, 1110, 1191
CseI GACGC 1 cut(s) 1021
CviAII CATG 9 cut(s) 404, 525, 632, 638, 644, 811, 933, 1070, 1146
DdeI CTNAG 3 cut(s) 186, 318, 1203
Eam1104I CTCTTC 2 cut(s) 599, 750
EarI CTCTTC 2 cut(s) 599, 750
Eco130I CCWWGG 2 cut(s) 18, 403
Eco47I GGWCC 2 cut(s) 1110, 1191
EcoO109I RGGNCCY 1 cut(s) 1110
EcoT14I CCWWGG 2 cut(s) 18, 403
ErhI CCWWGG 2 cut(s) 18, 403
Esp3I CGTCTC 1 cut(s) 1185
FaeI CATG 9 cut(s) 407, 528, 635, 641, 647, 814, 936, 1073, 1149
FalI AAGNNNNNCTT 4 cut(s) 16, 48, 203, 235
FatI CATG 9 cut(s) 403, 524, 631, 637, 643, 810, 932, 1069, 1145
FauI CCCGC 1 cut(s) 1056
FbaI TGATCA 4 cut(s) 628, 634, 640, 913
Fnu4HI GCNGC 4 cut(s) 30, 999, 1061, 1264
FokI GGATG 6 cut(s) 254, 722, 737, 756, 812, 1029
Fsp4HI GCNGC 4 cut(s) 30, 999, 1061, 1264
FspBI CTAG 3 cut(s) 195, 323, 959
GlaI GCGC 3 cut(s) 434, 1059, 1150
GluI GCNGC 4 cut(s) 30, 999, 1061, 1264
HaeIII GGCC 3 cut(s) 386, 538, 707
HapII CCGG 5 cut(s) 151, 983, 1130, 1173, 1267
HgaI GACGC 1 cut(s) 1021
HhaI GCGC 3 cut(s) 435, 1060, 1151
Hin1I GRCGYC 1 cut(s) 1013
Hin1II CATG 9 cut(s) 407, 528, 635, 641, 647, 814, 936, 1073, 1149
Hin6I GCGC 3 cut(s) 433, 1058, 1149
HinP1I GCGC 3 cut(s) 433, 1058, 1149
HincII GTYRAC 2 cut(s) 85, 108
HindII GTYRAC 2 cut(s) 85, 108
HinfI GANTC 5 cut(s) 92, 314, 1164, 1224, 1236
HpaII CCGG 5 cut(s) 151, 983, 1130, 1173, 1267
HphI GGTGA 3 cut(s) 436, 724, 1028
Hpy166II GTNNAC 4 cut(s) 85, 108, 732, 926
Hpy188I TCNGA 3 cut(s) 969, 1022, 1027
Hpy188III TCNNGA 8 cut(s) 132, 205, 220, 262, 632, 638, 644, 1070
Hpy8I GTNNAC 4 cut(s) 85, 108, 732, 926
Hpy99I CGWCG 1 cut(s) 1138
HpyAV CCTTC 8 cut(s) 166, 572, 662, 772, 910, 1049, 1056, 1250
HpyCH4III ACNGT 2 cut(s) 114, 565
HpyCH4IV ACGT 3 cut(s) 104, 952, 1136
HpyCH4V TGCA 2 cut(s) 926, 932
HpyF10VI GCNNNNNNNGC 1 cut(s) 1269
HpyF3I CTNAG 3 cut(s) 186, 318, 1203
HpySE526I ACGT 3 cut(s) 104, 952, 1136
Hsp92I GRCGYC 1 cut(s) 1013
Hsp92II CATG 9 cut(s) 407, 528, 635, 641, 647, 814, 936, 1073, 1149
HspAI GCGC 3 cut(s) 433, 1058, 1149
Ksp22I TGATCA 4 cut(s) 628, 634, 640, 913
LguI GCTCTTC 1 cut(s) 750
LmnI GCTCC 2 cut(s) 969, 1269
Lsp1109I GCAGC 2 cut(s) 16, 1047
LweI GCATC 3 cut(s) 454, 502, 873
MaeI CTAG 3 cut(s) 195, 323, 959
MaeII ACGT 3 cut(s) 104, 952, 1136
MaeIII GTNAC 1 cut(s) 442
MflI RGATCY 1 cut(s) 1027
MhlI GDGCHC 1 cut(s) 928
MluCI AATT 4 cut(s) 269, 331, 594, 1082
MlyI GAGTC 1 cut(s) 323
MmeI TCCRAC 2 cut(s) 36, 291
MroXI GAANNNNTTC 1 cut(s) 282
MseI TTAA 3 cut(s) 48, 215, 1106
MslI CAYNNNNRTG 4 cut(s) 636, 642, 921, 931
MspI CCGG 5 cut(s) 151, 983, 1130, 1173, 1267
MspR9I CCNGG 1 cut(s) 151
MvnI CGCG 4 cut(s) 435, 510, 998, 1151
MwoI GCNNNNNNNGC 1 cut(s) 1269
NciI CCSGG 1 cut(s) 151
NcoI CCATGG 1 cut(s) 403
NheI GCTAGC 1 cut(s) 322
NlaIII CATG 9 cut(s) 407, 528, 635, 641, 647, 814, 936, 1073, 1149
NlaIV GGNNCC 4 cut(s) 361, 1192, 1262, 1271
NmuCI GTSAC 1 cut(s) 442
NspI RCATGY 2 cut(s) 528, 936
OliI CACNNNNGTG 1 cut(s) 921
PagI TCATGA 4 cut(s) 631, 637, 643, 1069
PciSI GCTCTTC 1 cut(s) 750
PcsI WCGNNNNNNNCGW 3 cut(s) 110, 1013, 1245
PdmI GAANNNNTTC 1 cut(s) 282
PfeI GAWTC 4 cut(s) 92, 1164, 1224, 1236
PkrI GCNGC 4 cut(s) 31, 1000, 1062, 1265
PleI GAGTC 1 cut(s) 322
PpsI GAGTC 1 cut(s) 322
PpuMI RGGWCCY 1 cut(s) 1110
Psp1406I AACGTT 2 cut(s) 104, 952
Psp5II RGGWCCY 1 cut(s) 1110
PspN4I GGNNCC 4 cut(s) 361, 1192, 1262, 1271
PspPI GGNCC 3 cut(s) 705, 1110, 1191
PspPPI RGGWCCY 1 cut(s) 1110
PsuI RGATCY 1 cut(s) 1027
RseI CAYNNNNRTG 4 cut(s) 636, 642, 921, 931
SapI GCTCTTC 1 cut(s) 750
SaqAI TTAA 3 cut(s) 48, 215, 1106
SatI GCNGC 4 cut(s) 30, 999, 1061, 1264
Sau96I GGNCC 3 cut(s) 705, 1110, 1191
SchI GAGTC 1 cut(s) 323
ScrFI CCNGG 1 cut(s) 151
SduI GDGCHC 1 cut(s) 928
SfaNI GCATC 3 cut(s) 454, 502, 873
SinI GGWCC 2 cut(s) 1110, 1191
SmiMI CAYNNNNRTG 4 cut(s) 636, 642, 921, 931
Sse9I AATT 4 cut(s) 269, 331, 594, 1082
SsiI CCGC 6 cut(s) 998, 1001, 1063, 1151, 1194, 1264
SspMI CTAG 3 cut(s) 195, 323, 959
StyD4I CCNGG 1 cut(s) 149
StyI CCWWGG 2 cut(s) 18, 403
TaaI ACNGT 2 cut(s) 114, 565
TaiI ACGT 3 cut(s) 107, 955, 1139
TaqI TCGA 3 cut(s) 1007, 1115, 1199
TasI AATT 4 cut(s) 269, 331, 594, 1082
TauI GCSGC 2 cut(s) 1001, 1266
TfiI GAWTC 4 cut(s) 92, 1164, 1224, 1236
Tru1I TTAA 3 cut(s) 48, 215, 1106
Tru9I TTAA 3 cut(s) 48, 215, 1106
TscAI CASTG 1 cut(s) 1104
TseFI GTSAC 1 cut(s) 442
TseI GCWGC 2 cut(s) 29, 1060
Tsp45I GTSAC 1 cut(s) 442
TspDTI ATGAA 5 cut(s) 84, 185, 324, 892, 1031
TspGWI ACGGA 1 cut(s) 1173
TspRI CASTG 1 cut(s) 1104
VneI GTGCAC 1 cut(s) 924
VpaK11BI GGWCC 2 cut(s) 1110, 1191
XapI RAATTY 2 cut(s) 269, 331
XceI RCATGY 2 cut(s) 528, 936
XmnI GAANNNNTTC 1 cut(s) 282
XspI CTAG 3 cut(s) 195, 323, 959
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.