Rmu_sc0002505.1_g000016

26S proteasome non-ATPase regulatory subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002505.1
OG0013346
Physical Location & Seq
Forward (+)
63889 .. 66632
2744 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1017 bp
atggagatcgatcaatcggagccgcaaaccaaaagcgaagacctcttcaaagccgccgagaccggcgactcttcggcgttcgaatctctcactccaaagcagctcgcttcagcttcctctctcagaaacgaggacggccgctccctccttcacgtcgccgtctcttccggtcactctcaggtggtaaaggtactgctagctgctgatgaatcgagtggtgtgataaacagtaaggatgaagatggttgggcaccgctgcattctgcggcgagcagtgggaatgtagtaatagtggagatgctgctaagcaaaggagctgatgttaatttgaagaacgatggtggtcgcgttgcacttcactatgctgccagcaagggatggatggagattgctgaaaatttgatctcacatggggcgaagcttaatgtacaagacaaggttggttccaccccattgcatcgagcagctagtactgggaaatcacaattgtgtgaacttctaattgaggaaggagctgaaattgatgttgttgataaagcaggccagactcctcttatgagtgccgtgatttgcgagaacaaggagtgttatgatatgtttaacttcataggagctgatgttaatttgaagaacgatggtggtcgcgttgcacttcactatgctgccagcaagggatggatggagattgctgaaaatttgatctcacatggggcgaagcttaatgtacaagacaaggttggttccaccccattgcatcgagcagctagtactgggaaatcacaattgtgtgaacttctaattgaggaaggagctgaaattgatgttgttgataaagcaggccagactcctcttatgagtgccgtgatttgcgagaacaaggaggtttctctccttctaataaggcatggagcagatgtggatgtggaagacaaagaaggatacacagtgcttggtcgagcttcgaatgagttcagaccattattgattgatgccgctaaggcgatgcttgaagggtga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 Ank_3 Ank_4 Ank_5

Protein Analysis

338

Amino Acids

35.92

Weight (kDa)

4.88

Isoelectric Point (pI)

35.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 15 - 112 3.9e-17 Ankyrin repeats (3 copies)
Ank_5 PF13857 77 - 122 6.3e-06 Ankyrin repeats (many copies)
Ank PF00023 81 - 113 1.3e-08 Ankyrin repeat
Ank_4 PF13637 86 - 132 7.2e-09 Ankyrin repeats (many copies)
Ank_5 PF13857 101 - 152 5.2e-06 Ankyrin repeats (many copies)
Ank PF00023 115 - 146 6.6e-06 Ankyrin repeat
Ank_2 PF12796 119 - 211 4.1e-17 Ankyrin repeats (3 copies)
Ank_4 PF13637 119 - 168 1e-08 Ankyrin repeats (many copies)
Ank_5 PF13857 134 - 185 1.9e-06 Ankyrin repeats (many copies)
Ank PF00023 148 - 179 5.1e-06 Ankyrin repeat
Ank_2 PF12796 203 - 277 1.1e-14 Ankyrin repeats (3 copies)
Ank_5 PF13857 204 - 254 1.8e-06 Ankyrin repeats (many copies)
Ank PF00023 214 - 245 6.6e-06 Ankyrin repeat
Ank_4 PF13637 218 - 267 1e-08 Ankyrin repeats (many copies)
Ank_5 PF13857 233 - 284 1.7e-06 Ankyrin repeats (many copies)
Ank PF00023 247 - 278 5.1e-06 Ankyrin repeat
Ank_2 PF12796 252 - 321 2.3e-11 Ankyrin repeats (3 copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0013346)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G03430
fragaria_vesca FvH4_4g30470
malus_domestica MD13G1065800.v1.1 MD16G1064400.v1.1
prunus_persica Prupe.1G282400_v2.0.a1
pyrus_communis pycom13g05800 pycom16g05620
rosa_chinensis RchiOBHm_Chr4g0438821
rosa_laevigata RLG00000006328
rosa_multiflora Rmu_sc0002505.1_g000016
rosa_roxburghii Rroxscaffold_5G00379860
rosa_rugosa Rorug04G0313300
rosa_samantha Rh4AG364300 Rh4BG375400 Rh4CG389500 Rh4DG370000
rosa_wichuraiana Rw0G007240 Rw0G016790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 250
AccBSI CCGCTC 1 cut(s) 141
AccII CGCG 2 cut(s) 348, 645
AciI CCGC 6 cut(s) 23, 54, 139, 254, 266, 993
AcoI YGGCCR 1 cut(s) 136
AcsI RAATTY 2 cut(s) 397, 694
AcuI CTGAAG 1 cut(s) 93
AfaI GTAC 5 cut(s) 192, 429, 472, 726, 769
AgsI TTSAA 4 cut(s) 49, 331, 628, 1010
AjiI CACGTC 1 cut(s) 154
AleI CACNNNNGTG 2 cut(s) 487, 784
Alw26I GTCTC 2 cut(s) 53, 166
AoxI GGCC 3 cut(s) 136, 541, 838
ApeKI GCWGC 8 cut(s) 100, 200, 256, 301, 365, 464, 662, 761
ApoI RAATTY 2 cut(s) 397, 694
Asp700I GAANNNNTTC 1 cut(s) 968
AsuII TTCGAA 2 cut(s) 81, 962
AsuNHI GCTAGC 1 cut(s) 196
BaeGI GKGCMC 1 cut(s) 253
BanI GGYRCC 1 cut(s) 250
BbsI GAAGAC 2 cut(s) 45, 933
BbvI GCAGC 8 cut(s) 112, 187, 243, 288, 352, 476, 649, 773
BccI CCATC 7 cut(s) 236, 332, 372, 376, 629, 669, 673
BceAI ACGGC 4 cut(s) 143, 151, 548, 845
BciVI GTATCC 1 cut(s) 932
BcoDI GTCTC 2 cut(s) 53, 166
BfaI CTAG 3 cut(s) 197, 468, 765
BfuI GTATCC 1 cut(s) 932
BglI GCCNNNNNGGC 1 cut(s) 998
BlpI GCTNAGC 1 cut(s) 305
BmcAI AGTACT 2 cut(s) 472, 769
BmgBI CACGTC 1 cut(s) 154
BmiI GGNNCC 4 cut(s) 21, 252, 445, 742
BmrI ACTGGG 2 cut(s) 483, 780
BmsI GCATC 5 cut(s) 288, 466, 763, 979, 993
BmtI GCTAGC 1 cut(s) 200
BmuI ACTGGG 2 cut(s) 483, 780
BpiI GAAGAC 2 cut(s) 45, 933
Bpu10I CCTNAGC 1 cut(s) 996
Bpu1102I GCTNAGC 1 cut(s) 305
Bpu14I TTCGAA 2 cut(s) 81, 962
Bsa29I ATCGAT 1 cut(s) 9
BsaI GGTCTC 1 cut(s) 53
BsaWI WCCGGW 1 cut(s) 167
BsaXI ACNNNNNCTCC 8 cut(s) 125, 155, 306, 336, 603, 633, 900, 930
Bse118I RCCGGY 1 cut(s) 62
Bse1I ACTGG 2 cut(s) 478, 775
Bse3DI GCAATG 2 cut(s) 452, 749
BseCI ATCGAT 1 cut(s) 9
BseGI GGATG 6 cut(s) 241, 383, 387, 680, 684, 925
BseMI GCAATG 2 cut(s) 452, 749
BseMII CTCAG 2 cut(s) 136, 191
BseNI ACTGG 2 cut(s) 478, 775
BseRI GAGGAG 2 cut(s) 540, 837
BseSI GKGCMC 1 cut(s) 253
BseX3I CGGCCG 1 cut(s) 136
BseXI GCAGC 8 cut(s) 112, 187, 243, 288, 352, 476, 649, 773
Bsh1236I CGCG 2 cut(s) 348, 645
Bsh1285I CGRYCG 1 cut(s) 139
BshFI GGCC 3 cut(s) 138, 543, 840
BshNI GGYRCC 1 cut(s) 250
BshVI ATCGAT 1 cut(s) 9
BsiEI CGRYCG 1 cut(s) 139
BsiSI CCGG 2 cut(s) 63, 168
BsmAI GTCTC 2 cut(s) 53, 166
BsmBI CGTCTC 1 cut(s) 166
BsmI GAATGC 1 cut(s) 259
BsnI GGCC 3 cut(s) 138, 543, 840
Bso31I GGTCTC 1 cut(s) 53
Bsp119I TTCGAA 2 cut(s) 81, 962
Bsp1286I GDGCHC 1 cut(s) 253
Bsp1407I TGTACA 2 cut(s) 427, 724
Bsp143I GATC 4 cut(s) 6, 10, 402, 699
Bsp1720I GCTNAGC 1 cut(s) 305
BspACI CCGC 6 cut(s) 23, 54, 139, 254, 266, 993
BspANI GGCC 3 cut(s) 138, 543, 840
BspCNI CTCAG 2 cut(s) 135, 190
BspDI ATCGAT 1 cut(s) 9
BspFNI CGCG 2 cut(s) 348, 645
BspLI GGNNCC 4 cut(s) 21, 252, 445, 742
BspOI GCTAGC 1 cut(s) 200
BspT104I TTCGAA 2 cut(s) 81, 962
BspT107I GGYRCC 1 cut(s) 250
BspTNI GGTCTC 1 cut(s) 53
BsrBI CCGCTC 1 cut(s) 141
BsrDI GCAATG 2 cut(s) 452, 749
BsrFI RCCGGY 1 cut(s) 62
BsrGI TGTACA 2 cut(s) 427, 724
BsrI ACTGG 2 cut(s) 478, 775
BssAI RCCGGY 1 cut(s) 62
BssMI GATC 4 cut(s) 6, 10, 402, 699
Bst4CI ACNGT 2 cut(s) 230, 946
Bst6I CTCTTC 3 cut(s) 50, 76, 169
BstAUI TGTACA 2 cut(s) 427, 724
BstBI TTCGAA 2 cut(s) 81, 962
BstC8I GCNNGC 7 cut(s) 105, 198, 271, 370, 541, 667, 838
BstDEI CTNAG 4 cut(s) 122, 177, 305, 996
BstF5I GGATG 6 cut(s) 241, 383, 387, 680, 684, 925
BstFNI CGCG 2 cut(s) 348, 645
BstKTI GATC 4 cut(s) 9, 13, 405, 702
BstMAI GTCTC 2 cut(s) 53, 166
BstMBI GATC 4 cut(s) 6, 10, 402, 699
BstMCI CGRYCG 1 cut(s) 139
BstMWI GCNNNNNNNGC 1 cut(s) 998
BstSLI GKGCMC 1 cut(s) 253
BstUI CGCG 2 cut(s) 348, 645
BstV1I GCAGC 8 cut(s) 112, 187, 243, 288, 352, 476, 649, 773
BstV2I GAAGAC 2 cut(s) 45, 933
BstZI CGGCCG 1 cut(s) 136
Bsu15I ATCGAT 1 cut(s) 9
BsuI GTATCC 1 cut(s) 932
BsuRI GGCC 3 cut(s) 138, 543, 840
BsuTUI ATCGAT 1 cut(s) 9
BtrI CACGTC 1 cut(s) 154
BtsCI GGATG 6 cut(s) 241, 383, 387, 680, 684, 925
BtsI GCAGTG 1 cut(s) 280
BtsIMutI CAGTG 2 cut(s) 280, 951
Cac8I GCNNGC 7 cut(s) 105, 198, 271, 370, 541, 667, 838
Cfr10I RCCGGY 1 cut(s) 62
ClaI ATCGAT 1 cut(s) 9
Csp6I GTAC 5 cut(s) 191, 428, 471, 725, 768
CviAII CATG 3 cut(s) 410, 707, 905
CviQI GTAC 5 cut(s) 191, 428, 471, 725, 768
DdeI CTNAG 4 cut(s) 122, 177, 305, 996
DpnI GATC 4 cut(s) 8, 12, 404, 701
DpnII GATC 4 cut(s) 6, 10, 402, 699
EaeI YGGCCR 1 cut(s) 136
EagI CGGCCG 1 cut(s) 136
Eam1104I CTCTTC 3 cut(s) 50, 76, 169
EarI CTCTTC 3 cut(s) 50, 76, 169
EclXI CGGCCG 1 cut(s) 136
Eco31I GGTCTC 1 cut(s) 53
Eco52I CGGCCG 1 cut(s) 136
Eco57I CTGAAG 1 cut(s) 93
Esp3I CGTCTC 1 cut(s) 166
FaeI CATG 3 cut(s) 413, 710, 908
FatI CATG 3 cut(s) 409, 706, 904
FokI GGATG 6 cut(s) 248, 390, 394, 687, 691, 932
FspBI CTAG 3 cut(s) 197, 468, 765
HaeIII GGCC 3 cut(s) 138, 543, 840
HapII CCGG 2 cut(s) 63, 168
Hin1II CATG 3 cut(s) 413, 710, 908
HindIII AAGCTT 2 cut(s) 419, 716
HinfI GANTC 5 cut(s) 68, 83, 209, 547, 844
HpaII CCGG 2 cut(s) 63, 168
Hpy166II GTNNAC 2 cut(s) 494, 791
Hpy188I TCNGA 3 cut(s) 19, 125, 974
Hpy8I GTNNAC 2 cut(s) 494, 791
Hpy99I CGWCG 1 cut(s) 158
HpyAV CCTTC 6 cut(s) 158, 503, 800, 902, 929, 1004
HpyCH4III ACNGT 2 cut(s) 230, 946
HpyCH4IV ACGT 1 cut(s) 153
HpyCH4V TGCA 5 cut(s) 259, 353, 457, 650, 754
HpyF10VI GCNNNNNNNGC 1 cut(s) 998
HpyF3I CTNAG 4 cut(s) 122, 177, 305, 996
HpySE526I ACGT 1 cut(s) 153
Hsp92II CATG 3 cut(s) 413, 710, 908
Kzo9I GATC 4 cut(s) 6, 10, 402, 699
LmnI GCTCC 7 cut(s) 19, 146, 314, 512, 611, 809, 908
Lsp1109I GCAGC 8 cut(s) 112, 187, 243, 288, 352, 476, 649, 773
LweI GCATC 5 cut(s) 288, 466, 763, 979, 993
MaeI CTAG 3 cut(s) 197, 468, 765
MaeII ACGT 1 cut(s) 153
MaeIII GTNAC 1 cut(s) 170
MalI GATC 4 cut(s) 8, 12, 404, 701
MbiI CCGCTC 1 cut(s) 141
MboI GATC 4 cut(s) 6, 10, 402, 699
MboII GAAGA 8 cut(s) 37, 50, 63, 156, 251, 343, 640, 938
MfeI CAATTG 2 cut(s) 485, 782
MhlI GDGCHC 1 cut(s) 253
MlyI GAGTC 3 cut(s) 62, 541, 838
MnlI CCTC 9 cut(s) 53, 124, 127, 155, 499, 561, 796, 858, 874
MroXI GAANNNNTTC 1 cut(s) 968
MseI TTAA 5 cut(s) 324, 423, 600, 621, 720
MslI CAYNNNNRTG 2 cut(s) 487, 784
MspA1I CMGCKG 1 cut(s) 256
MspI CCGG 2 cut(s) 63, 168
MunI CAATTG 2 cut(s) 485, 782
Mva1269I GAATGC 1 cut(s) 259
MvnI CGCG 2 cut(s) 348, 645
MwoI GCNNNNNNNGC 1 cut(s) 998
NdeII GATC 4 cut(s) 6, 10, 402, 699
NheI GCTAGC 1 cut(s) 196
NlaIII CATG 3 cut(s) 413, 710, 908
NlaIV GGNNCC 4 cut(s) 21, 252, 445, 742
NmeAIII GCCGAG 1 cut(s) 82
NmuCI GTSAC 1 cut(s) 170
NspV TTCGAA 2 cut(s) 81, 962
OliI CACNNNNGTG 2 cut(s) 487, 784
PctI GAATGC 1 cut(s) 259
PdmI GAANNNNTTC 1 cut(s) 968
PfeI GAWTC 2 cut(s) 83, 209
PleI GAGTC 3 cut(s) 62, 541, 838
PpsI GAGTC 3 cut(s) 62, 541, 838
PspN4I GGNNCC 4 cut(s) 21, 252, 445, 742
RsaI GTAC 5 cut(s) 192, 429, 472, 726, 769
RsaNI GTAC 5 cut(s) 191, 428, 471, 725, 768
RseI CAYNNNNRTG 2 cut(s) 487, 784
SaqAI TTAA 5 cut(s) 324, 423, 600, 621, 720
Sau3AI GATC 4 cut(s) 6, 10, 402, 699
ScaI AGTACT 2 cut(s) 472, 769
SchI GAGTC 3 cut(s) 62, 541, 838
SduI GDGCHC 1 cut(s) 253
SfaNI GCATC 5 cut(s) 288, 466, 763, 979, 993
SfuI TTCGAA 2 cut(s) 81, 962
SmiMI CAYNNNNRTG 2 cut(s) 487, 784
SsiI CCGC 6 cut(s) 23, 54, 139, 254, 266, 993
SspMI CTAG 3 cut(s) 197, 468, 765
TaaI ACNGT 2 cut(s) 230, 946
TaiI ACGT 1 cut(s) 156
TaqI TCGA 7 cut(s) 9, 81, 212, 460, 757, 955, 962
TatI WGTACW 4 cut(s) 427, 470, 724, 767
TauI GCSGC 5 cut(s) 25, 56, 141, 269, 995
TfiI GAWTC 2 cut(s) 83, 209
Tru1I TTAA 5 cut(s) 324, 423, 600, 621, 720
Tru9I TTAA 5 cut(s) 324, 423, 600, 621, 720
TscAI CASTG 2 cut(s) 280, 951
TseFI GTSAC 1 cut(s) 170
TseI GCWGC 8 cut(s) 100, 200, 256, 301, 365, 464, 662, 761
Tsp45I GTSAC 1 cut(s) 170
TspDTI ATGAA 3 cut(s) 222, 252, 595
TspRI CASTG 2 cut(s) 280, 951
XapI RAATTY 2 cut(s) 397, 694
XmnI GAANNNNTTC 1 cut(s) 968
XspI CTAG 3 cut(s) 197, 468, 765
ZrmI AGTACT 2 cut(s) 472, 769
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.