Rmu_sc0002918.1_g000012

Cotton fibre expressed protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002918.1
OG0010777
Physical Location & Seq
Forward (+)
110841 .. 115167
4327 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2097 bp
atgagagttagagcggcggcttggttgacggtgtcaagttctcgtgctgggaagatcggaggggcctcggttgttgcggtggtagtggcaacggagcgaggcttctcattctcctcctcgcccgtttcaagtgcatctatgcaaggcaaaaccttctgcttttctttctcgtcgcttgatgctggtggtgtagaagaacatgctcacggcggaggcatggttgctgaggcggttgtgcaatggagaaatctgctaggatttctccgaggtggatttccaactgggcctactgggttgtcatggatgtgggcctttcttttggctattggcaataagggcttcattctctccatgagttcttcaatggcagagccagaatcagagtcttacaccaaaccccaaaagttggtctcatcagatcaaaaccatgaaaacccaagtaagttctacaatcattttctctacaaagttgctctagttgtagttttcttcgttattctccctctttttccatcacaagctcctgagttcataaaccaaagtgttgtcaccagaagctgggagcttgttcatctcctcctagttggtatagccgtctcttatggcttatttagcagactaaatgaaaaagtagagaaagaaaacaacacgaaattcgataatgctcattcctatgtgtctagaattcttgaggtgtcctcagtttttgatgatgaggcagaaacctcacctggacctgatgaaaacaaggtccaggcatggagtagtcagtattttaggaatgaacctgtggtggttgtggctcaagaacactctgttcttgatgagcagagagatagcagttcaatacttggtgaaaagccattgcttttgcctgttaggagcctaaaacaacgtgttcctgaccaggacaacatagagtccgttaatgaatctactgttaattctggtggtgctctgagtggctctaattctaggtctggttcaagaaggttttcgagcaaatcgagtaaggctaaagctggtgaaattgggggtctagatcatcaagaattggaggagaaattgaaggagagtgttgtgcttccttctccaattccgtggcgatcaagatcaggaaggttggaagtaaaggaggatcttgttagtagtactccaatggaggaagctgaatttaaccgcctagatcctcggggtgtttcgaggtcccaaagctctcgttcatctcgatccgagtcagtgtcttcatcaccaaagctgtctccttcgacatcacttttgtctccaaagaagttatctcctgcaccttccttgtcatcagaagctcaagccaagagtgttgaggatgtggggaggaagaagagcttttacaagacttctattcccccacctcctccgcccccaccgttgttttataaatcatcatcattgaggccaagcagtgatgaggtttcgtatgagaaggatttgaggagaagcttgactagtgaagcaaagaacttgaatagaagcaatggagaatttatgatgggcagagttaattcaggggttgaaacaaagcataggctgagccatgttgatggcatttcaatgggaaagtctgttagaacaaccagagctggagaacaaggatatgtaaatgggaaggtggagcaaagcgctaaggaagttgaagctaatttggttgagaatgtaacaagaaaaagggtggggttcgatgaatcttcattctggattgagcaattgagccataaaagcagcattcccaacaaccacaagtcatcagcttttcaggaattttccgaggaagatgaaaaggaaaatcttttcgataaggagcttatggaatcggatgaagaaaccgaaagcgaggatgacgacactgaaggaggtttttctcctaaagacattggagagagcccaaagtcaagtccaaggccctatcaacctgtttctgggagtgcaagtgatggagggcctgatgtggataagaaggcagatgaattcatagctaaattcagggagcagattcggcttcagagaatagattcgatcaagaaatcgagtgctcagattagtaaaaacttgtcgaggtaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF761

Protein Analysis

698

Amino Acids

76.6

Weight (kDa)

6.07

Isoelectric Point (pI)

61.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF761 PF05553 660 - 690 2e-13 Cotton fibre expressed protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1416
AccB7I CCANNNNNTGG 2 cut(s) 406, 558
AccBSI CCGCTC 1 cut(s) 14
AciI CCGC 7 cut(s) 14, 17, 77, 210, 230, 1180, 1397
AclWI GGATC 3 cut(s) 1146, 1181, 1224
AcsI RAATTY 7 cut(s) 653, 684, 1172, 1520, 1796, 2003, 2015
AcuI CTGAAG 2 cut(s) 1905, 2021
AfaI GTAC 1 cut(s) 1153
AfeI AGCGCT 1 cut(s) 1660
AfiI CCNNNNNNNGG 3 cut(s) 406, 558, 1955
AflIII ACRYGT 1 cut(s) 895
AgsI TTSAA 9 cut(s) 129, 363, 846, 987, 1069, 1504, 1553, 1590, 1673
AhlI ACTAGT 1 cut(s) 1484
AjnI CCWGG 3 cut(s) 730, 753, 906
Alw21I GWGCWC 2 cut(s) 958, 2071
Alw26I GTCTC 4 cut(s) 415, 601, 1266, 1287
AlwI GGATC 3 cut(s) 1146, 1181, 1224
AlwNI CAGNNNCTG 1 cut(s) 558
Ama87I CYCGRG 1 cut(s) 1191
Aor51HI AGCGCT 1 cut(s) 1660
AoxI GGCC 6 cut(s) 63, 284, 309, 1433, 1937, 1976
ApeKI GCWGC 1 cut(s) 1758
ApoI RAATTY 7 cut(s) 653, 684, 1172, 1520, 1796, 2003, 2015
ArsI GACNNNNNNTTYG 2 cut(s) 1261, 1293
Asp700I GAANNNNTTC 2 cut(s) 994, 2047
AspLEI GCGC 1 cut(s) 1661
AspS9I GGNCC 8 cut(s) 63, 284, 309, 734, 751, 1206, 1938, 1976
AsuHPI GGTGA 5 cut(s) 541, 720, 866, 1037, 1242
AvaI CYCGRG 1 cut(s) 1191
AvaII GGWCC 3 cut(s) 734, 751, 1206
BanII GRGCYC 1 cut(s) 1922
BarI GAAGNNNNNNTAC 2 cut(s) 1352, 1384
BauI CACGAG 1 cut(s) 42
BbsI GAAGAC 1 cut(s) 1236
Bbv12I GWGCWC 2 cut(s) 958, 2071
BbvCI CCTCAGC 1 cut(s) 225
BbvI GCAGC 1 cut(s) 1770
BccI CCATC 4 cut(s) 520, 1522, 1574, 1964
BceAI ACGGC 2 cut(s) 223, 578
BciT130I CCWGG 3 cut(s) 732, 755, 908
BcoDI GTCTC 4 cut(s) 415, 601, 1266, 1287
BcuI ACTAGT 1 cut(s) 1484
BfaI CTAG 8 cut(s) 254, 476, 581, 681, 975, 1040, 1184, 1485
BfoI RGCGCY 1 cut(s) 1662
BisI GCNGC 3 cut(s) 15, 18, 1759
BlpI GCTNAGC 1 cut(s) 1568
BlsI GCNGC 3 cut(s) 16, 19, 1760
BmcAI AGTACT 1 cut(s) 1153
Bme1390I CCNGG 3 cut(s) 732, 755, 908
Bme18I GGWCC 3 cut(s) 734, 751, 1206
BmeT110I CYCGRG 1 cut(s) 1191
BmgT120I GGNCC 8 cut(s) 63, 284, 309, 734, 751, 1206, 1938, 1976
BmiI GGNNCC 3 cut(s) 64, 884, 1208
BmrFI CCNGG 3 cut(s) 732, 755, 908
BmrI ACTGGG 2 cut(s) 291, 300
BmsI GCATC 2 cut(s) 143, 169
BmuI ACTGGG 2 cut(s) 291, 300
BpiI GAAGAC 1 cut(s) 1236
BplI GAGNNNNNCTC 2 cut(s) 683, 715
BpmI CTGGAG 1 cut(s) 1641
Bpu10I CCTNAGC 2 cut(s) 225, 1662
Bpu1102I GCTNAGC 1 cut(s) 1568
BpuEI CTTGAG 3 cut(s) 710, 789, 1311
BsaBI GATNNNNATC 1 cut(s) 1111
BsaI GGTCTC 1 cut(s) 415
BsaJI CCNNGG 6 cut(s) 66, 265, 1100, 1190, 1803, 1934
BsaXI ACNNNNNCTCC 4 cut(s) 204, 234, 1951, 1981
Bsc4I CCNNNNNNNGG 3 cut(s) 406, 558, 1955
Bse1I ACTGG 2 cut(s) 286, 295
Bse3DI GCAATG 3 cut(s) 245, 863, 1519
Bse8I GATNNNNATC 1 cut(s) 1111
BseBI CCWGG 3 cut(s) 732, 755, 908
BseDI CCNNGG 6 cut(s) 66, 265, 1100, 1190, 1803, 1934
BseGI GGATG 4 cut(s) 309, 1351, 1858, 1879
BseJI GATNNNNATC 1 cut(s) 1111
BseLI CCNNNNNNNGG 3 cut(s) 406, 558, 1955
BseMI GCAATG 3 cut(s) 245, 863, 1519
BseMII CTCAG 6 cut(s) 216, 516, 714, 950, 1559, 2084
BseNI ACTGG 2 cut(s) 286, 295
BseRI GAGGAG 6 cut(s) 103, 106, 566, 1073, 1383, 1486
BseXI GCAGC 1 cut(s) 1770
BseYI CCCAGC 2 cut(s) 47, 558
BsgI GTGCAG 1 cut(s) 1287
BshFI GGCC 6 cut(s) 65, 286, 311, 1435, 1939, 1978
BsiHKAI GWGCWC 2 cut(s) 958, 2071
BsiHKCI CYCGRG 1 cut(s) 1191
BslFI GGGAC 1 cut(s) 1192
BslI CCNNNNNNNGG 3 cut(s) 406, 558, 1955
BsmAI GTCTC 4 cut(s) 415, 601, 1266, 1287
BsmBI CGTCTC 1 cut(s) 601
BsmFI GGGAC 1 cut(s) 1192
BsmI GAATGC 1 cut(s) 1761
BsnI GGCC 6 cut(s) 65, 286, 311, 1435, 1939, 1978
Bso31I GGTCTC 1 cut(s) 415
BsoBI CYCGRG 1 cut(s) 1191
Bsp1286I GDGCHC 3 cut(s) 958, 1922, 2071
Bsp143I GATC 9 cut(s) 54, 418, 1042, 1106, 1112, 1138, 1186, 1229, 2052
Bsp1720I GCTNAGC 1 cut(s) 1568
BspACI CCGC 7 cut(s) 14, 17, 77, 210, 230, 1180, 1397
BspANI GGCC 6 cut(s) 65, 286, 311, 1435, 1939, 1978
BspCNI CTCAG 6 cut(s) 217, 517, 713, 951, 1560, 2083
BspLI GGNNCC 3 cut(s) 64, 884, 1208
BspPI GGATC 3 cut(s) 1146, 1181, 1224
BspQI GCTCTTC 1 cut(s) 1355
BspTNI GGTCTC 1 cut(s) 415
BsrBI CCGCTC 1 cut(s) 14
BsrDI GCAATG 3 cut(s) 245, 863, 1519
BsrI ACTGG 2 cut(s) 286, 295
BssECI CCNNGG 6 cut(s) 66, 265, 1100, 1190, 1803, 1934
BssMI GATC 9 cut(s) 54, 418, 1042, 1106, 1112, 1138, 1186, 1229, 2052
BssSI CACGAG 1 cut(s) 42
BssT1I CCWWGG 1 cut(s) 1934
Bst2BI CACGAG 1 cut(s) 42
Bst2UI CCWGG 3 cut(s) 732, 755, 908
Bst4CI ACNGT 3 cut(s) 31, 940, 1407
Bst6I CTCTTC 1 cut(s) 1355
BstDEI CTNAG 7 cut(s) 225, 525, 700, 959, 1568, 1662, 2070
BstDSI CCRYGG 1 cut(s) 1100
BstF5I GGATG 4 cut(s) 309, 1351, 1858, 1879
BstH2I RGCGCY 1 cut(s) 1662
BstHHI GCGC 1 cut(s) 1661
BstKTI GATC 9 cut(s) 57, 421, 1045, 1109, 1115, 1141, 1189, 1232, 2055
BstMAI GTCTC 4 cut(s) 415, 601, 1266, 1287
BstMBI GATC 9 cut(s) 54, 418, 1042, 1106, 1112, 1138, 1186, 1229, 2052
BstMWI GCNNNNNNNGC 4 cut(s) 336, 612, 1755, 2032
BstNI CCWGG 3 cut(s) 732, 755, 908
BstNSI RCATGY 1 cut(s) 203
BstSCI CCNGG 3 cut(s) 730, 753, 906
BstV1I GCAGC 1 cut(s) 1770
BstV2I GAAGAC 1 cut(s) 1236
BstX2I RGATCY 2 cut(s) 1138, 1186
BstXI CCANNNNNNTGG 2 cut(s) 1101, 1580
BstYI RGATCY 2 cut(s) 1138, 1186
BsuRI GGCC 6 cut(s) 65, 286, 311, 1435, 1939, 1978
BtgI CCRYGG 1 cut(s) 1100
BtsCI GGATG 4 cut(s) 309, 1351, 1858, 1879
BtsI GCAGTG 1 cut(s) 1447
BtsIMutI CAGTG 3 cut(s) 1245, 1447, 1881
CaiI CAGNNNCTG 1 cut(s) 558
CfoI GCGC 1 cut(s) 1661
Cfr13I GGNCC 8 cut(s) 63, 284, 309, 734, 751, 1206, 1938, 1976
Csp6I GTAC 1 cut(s) 1152
CviAII CATG 7 cut(s) 200, 217, 300, 352, 428, 759, 1574
CviQI GTAC 1 cut(s) 1152
DdeI CTNAG 7 cut(s) 225, 525, 700, 959, 1568, 1662, 2070
DpnI GATC 9 cut(s) 56, 420, 1044, 1108, 1114, 1140, 1188, 1231, 2054
DpnII GATC 9 cut(s) 54, 418, 1042, 1106, 1112, 1138, 1186, 1229, 2052
Eam1104I CTCTTC 1 cut(s) 1355
EarI CTCTTC 1 cut(s) 1355
EciI GGCGGA 2 cut(s) 225, 1386
Eco130I CCWWGG 1 cut(s) 1934
Eco24I GRGCYC 1 cut(s) 1922
Eco31I GGTCTC 1 cut(s) 415
Eco47I GGWCC 3 cut(s) 734, 751, 1206
Eco47III AGCGCT 1 cut(s) 1660
Eco57I CTGAAG 2 cut(s) 1905, 2021
Eco88I CYCGRG 1 cut(s) 1191
EcoO109I RGGNCCY 4 cut(s) 63, 1206, 1938, 1976
EcoRI GAATTC 2 cut(s) 684, 2003
EcoRII CCWGG 3 cut(s) 730, 753, 906
EcoT14I CCWWGG 1 cut(s) 1934
EcoT38I GRGCYC 1 cut(s) 1922
ErhI CCWWGG 1 cut(s) 1934
Esp3I CGTCTC 1 cut(s) 601
FaeI CATG 7 cut(s) 203, 220, 303, 355, 431, 762, 1577
FalI AAGNNNNNCTT 2 cut(s) 1349, 1381
FaqI GGGAC 1 cut(s) 1192
FatI CATG 7 cut(s) 199, 216, 299, 351, 427, 758, 1573
Fnu4HI GCNGC 3 cut(s) 15, 18, 1759
FokI GGATG 4 cut(s) 316, 1358, 1865, 1886
FriOI GRGCYC 1 cut(s) 1922
Fsp4HI GCNGC 3 cut(s) 15, 18, 1759
FspBI CTAG 8 cut(s) 254, 476, 581, 681, 975, 1040, 1184, 1485
GlaI GCGC 1 cut(s) 1660
GluI GCNGC 3 cut(s) 15, 18, 1759
GsaI CCCAGC 2 cut(s) 51, 562
GsuI CTGGAG 1 cut(s) 1641
HaeII RGCGCY 1 cut(s) 1662
HaeIII GGCC 6 cut(s) 65, 286, 311, 1435, 1939, 1978
HhaI GCGC 1 cut(s) 1661
Hin1II CATG 7 cut(s) 203, 220, 303, 355, 431, 762, 1577
Hin6I GCGC 1 cut(s) 1659
HinP1I GCGC 1 cut(s) 1659
HincII GTYRAC 1 cut(s) 27
HindII GTYRAC 1 cut(s) 27
HindIII AAGCTT 1 cut(s) 1477
HinfI GANTC 9 cut(s) 377, 383, 920, 932, 1235, 1721, 1847, 2029, 2048
HphI GGTGA 5 cut(s) 541, 720, 866, 1037, 1242
Hpy166II GTNNAC 1 cut(s) 27
Hpy8I GTNNAC 1 cut(s) 27
Hpy99I CGWCG 1 cut(s) 175
HpyCH4III ACNGT 3 cut(s) 31, 940, 1407
HpyCH4IV ACGT 1 cut(s) 895
HpyCH4V TGCA 5 cut(s) 134, 142, 238, 1304, 1964
HpyF10VI GCNNNNNNNGC 4 cut(s) 336, 612, 1755, 2032
HpyF3I CTNAG 7 cut(s) 225, 525, 700, 959, 1568, 1662, 2070
HpySE526I ACGT 1 cut(s) 895
Hsp92II CATG 7 cut(s) 203, 220, 303, 355, 431, 762, 1577
HspAI GCGC 1 cut(s) 1659
Kzo9I GATC 9 cut(s) 54, 418, 1042, 1106, 1112, 1138, 1186, 1229, 2052
LguI GCTCTTC 1 cut(s) 1355
LmnI GCTCC 7 cut(s) 94, 526, 562, 882, 1651, 1837, 2023
Lsp1109I GCAGC 1 cut(s) 1770
LweI GCATC 2 cut(s) 143, 169
MaeI CTAG 8 cut(s) 254, 476, 581, 681, 975, 1040, 1184, 1485
MaeII ACGT 1 cut(s) 895
MaeIII GTNAC 2 cut(s) 547, 1693
MalI GATC 9 cut(s) 56, 420, 1044, 1108, 1114, 1140, 1188, 1231, 2054
MbiI CCGCTC 1 cut(s) 14
MboI GATC 9 cut(s) 54, 418, 1042, 1106, 1112, 1138, 1186, 1229, 2052
MfeI CAATTG 1 cut(s) 1742
MflI RGATCY 2 cut(s) 1138, 1186
MhlI GDGCHC 3 cut(s) 958, 1922, 2071
MlyI GAGTC 3 cut(s) 392, 929, 1244
MmeI TCCRAC 2 cut(s) 302, 1104
MroXI GAANNNNTTC 2 cut(s) 994, 2047
MseI TTAA 4 cut(s) 927, 942, 1176, 1539
MslI CAYNNNNRTG 4 cut(s) 304, 672, 1578, 1589
MspR9I CCNGG 3 cut(s) 732, 755, 908
MunI CAATTG 1 cut(s) 1742
Mva1269I GAATGC 1 cut(s) 1761
MvaI CCWGG 3 cut(s) 732, 755, 908
MwoI GCNNNNNNNGC 4 cut(s) 336, 612, 1755, 2032
NdeII GATC 9 cut(s) 54, 418, 1042, 1106, 1112, 1138, 1186, 1229, 2052
NlaIII CATG 7 cut(s) 203, 220, 303, 355, 431, 762, 1577
NlaIV GGNNCC 3 cut(s) 64, 884, 1208
NmuCI GTSAC 1 cut(s) 547
NspI RCATGY 1 cut(s) 203
PciSI GCTCTTC 1 cut(s) 1355
PcsI WCGNNNNNNNCGW 2 cut(s) 1004, 1225
PctI GAATGC 1 cut(s) 1761
PdmI GAANNNNTTC 2 cut(s) 994, 2047
PfeI GAWTC 6 cut(s) 377, 932, 1721, 1847, 2029, 2048
PflFI GACNNNGTC 1 cut(s) 31
PflMI CCANNNNNTGG 2 cut(s) 406, 558
PkrI GCNGC 3 cut(s) 16, 19, 1760
PleI GAGTC 3 cut(s) 391, 928, 1243
PpsI GAGTC 3 cut(s) 391, 928, 1243
PpuMI RGGWCCY 1 cut(s) 1206
PsiI TTATAA 1 cut(s) 1416
Psp5II RGGWCCY 1 cut(s) 1206
Psp6I CCWGG 3 cut(s) 730, 753, 906
PspFI CCCAGC 2 cut(s) 47, 558
PspGI CCWGG 3 cut(s) 730, 753, 906
PspN4I GGNNCC 3 cut(s) 64, 884, 1208
PspPI GGNCC 8 cut(s) 63, 284, 309, 734, 751, 1206, 1938, 1976
PspPPI RGGWCCY 1 cut(s) 1206
PstNI CAGNNNCTG 1 cut(s) 558
PsuI RGATCY 2 cut(s) 1138, 1186
PsyI GACNNNGTC 1 cut(s) 31
RsaI GTAC 1 cut(s) 1153
RsaNI GTAC 1 cut(s) 1152
RseI CAYNNNNRTG 4 cut(s) 304, 672, 1578, 1589
SapI GCTCTTC 1 cut(s) 1355
SaqAI TTAA 4 cut(s) 927, 942, 1176, 1539
SatI GCNGC 3 cut(s) 15, 18, 1759
Sau3AI GATC 9 cut(s) 54, 418, 1042, 1106, 1112, 1138, 1186, 1229, 2052
Sau96I GGNCC 8 cut(s) 63, 284, 309, 734, 751, 1206, 1938, 1976
ScaI AGTACT 1 cut(s) 1153
SchI GAGTC 3 cut(s) 392, 929, 1244
ScrFI CCNGG 3 cut(s) 732, 755, 908
SduI GDGCHC 3 cut(s) 958, 1922, 2071
SfaNI GCATC 2 cut(s) 143, 169
SinI GGWCC 3 cut(s) 734, 751, 1206
SmiMI CAYNNNNRTG 4 cut(s) 304, 672, 1578, 1589
SmlI CTYRAG 3 cut(s) 689, 804, 1326
SmoI CTYRAG 3 cut(s) 689, 804, 1326
SpeI ACTAGT 1 cut(s) 1484
SsiI CCGC 7 cut(s) 14, 17, 77, 210, 230, 1180, 1397
SspMI CTAG 8 cut(s) 254, 476, 581, 681, 975, 1040, 1184, 1485
StyD4I CCNGG 3 cut(s) 730, 753, 906
StyI CCWWGG 1 cut(s) 1934
TaaI ACNGT 3 cut(s) 31, 940, 1407
TaiI ACGT 1 cut(s) 898
TatI WGTACW 1 cut(s) 1151
TauI GCSGC 2 cut(s) 17, 20
TfiI GAWTC 6 cut(s) 377, 932, 1721, 1847, 2029, 2048
Tru1I TTAA 4 cut(s) 927, 942, 1176, 1539
Tru9I TTAA 4 cut(s) 927, 942, 1176, 1539
TscAI CASTG 3 cut(s) 1245, 1447, 1888
TseFI GTSAC 1 cut(s) 547
TseI GCWGC 1 cut(s) 1758
Tsp45I GTSAC 1 cut(s) 547
TspGWI ACGGA 3 cut(s) 107, 913, 1089
TspRI CASTG 3 cut(s) 1245, 1447, 1888
Tth111I GACNNNGTC 1 cut(s) 31
Van91I CCANNNNNTGG 2 cut(s) 406, 558
VpaK11BI GGWCC 3 cut(s) 734, 751, 1206
XapI RAATTY 7 cut(s) 653, 684, 1172, 1520, 1796, 2003, 2015
XbaI TCTAGA 2 cut(s) 680, 1039
XceI RCATGY 1 cut(s) 203
XmnI GAANNNNTTC 2 cut(s) 994, 2047
XspI CTAG 8 cut(s) 254, 476, 581, 681, 975, 1040, 1184, 1485
ZrmI AGTACT 1 cut(s) 1153
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.