Rmu_sc0003086.1_g000001

chromosome transmission fidelity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003086.1
OG0000880
Physical Location & Seq
Reverse (-)
1 .. 6091
6091 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0003086.1_g000001.1.cds

Sequence Viewer

Length: 1457 bp
atggacatggacatggagatggagatggacatggacatggacgagctcgaattgcttgaagccaactatcagctccacgaagattgcacggagctcgaaccgccggaaccctatccagaagaagaagaccaacagcctaccggttccaaatcgccaccgtcgactgaccaccagcagtctactgacacccaaatcaacggccacaagcgtctgcgaccagaagacgaaactgccgaagctcccggaccatctgacgagaaacggagcaggatcgaagatgaggattggcttcgttactcgccgccacaccagatcgatcctgttgttgaagagacaagtgctgatcttgtggaggagaagattgtgtctaggtatgcctcggagatcgacggtgacttcattccggtgacggcgccgggtttgggcgatagggtttatgccaagatttctagggtggagatggaggagcgtccacagagattgcatggaactggacaatctcaatctggaggtctcatttcggaacctattgatgttctactgcaaagactggagcaagaggcatttacaagggccttgcaagccagttccagaggtcaaagtgatgtgattcttcccgaaacaccagttgtgcatgaacgactttgggtggataaatatgctccaaactcatttactgagcttctcagcgatgaacagacaaatcgtgaggtcctgctttggctaaaacaatgggattcttgtgtttttggatcagagattaggagcacatcagatgaggttttgtctgctttaaaacgacattcttccatcactcaacatcaaaaactttccgagtcaaagtttccgagaagtaacaaagtatccggatggaataatgaaaaaattaagcatttcaatggtatgaatcatgaaaatactaattcaaaaagcgttcaagagttgtcaaacaattcccggtctactggtccgccagaacaaaagatcctcttactttgtggcccaccagggctcggaaagactacacttgcacatgtggctgcaaagcattgtggataccgagtggtggaggtgaatgctagtgatgatcgatcatcatcaactattggagctaaaatacttgatgttgttcagatgaactcagtctcagccgactcaaggccaaagtgtttggtgattgacgaaatagatggggcacttggtgacggaaaaggtgcggttgaagtcattctaaaaatggtttctgctgataagaagtctgatatggagaaggaaaatgttaataaagaacaaacatcaggaaaggcatcttcaaaaaaggggcggaaatctgcatccttgttaagacctattatttgcatatgcaatgacctatatgcaccttcgttgagaccattacgtcagatagcgaaggttcatgtttttgttcaaccaacagtgaatcgagtggtgagcag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AAA

Protein Analysis

485

Amino Acids

54.09

Weight (kDa)

5.06

Isoelectric Point (pI)

57.82

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1398
AccB1I GGYRCC 1 cut(s) 412
AccI GTMKAC 3 cut(s) 161, 179, 962
AccIII TCCGGA 1 cut(s) 866
AciI CCGC 5 cut(s) 101, 302, 971, 1217, 1324
AclWI GGATC 4 cut(s) 278, 311, 760, 979
AcoI YGGCCR 1 cut(s) 199
AcyI GRCGYC 1 cut(s) 413
AdeI CACNNNGTG 1 cut(s) 1202
AfiI CCNNNNNNNGG 4 cut(s) 422, 1013, 1066, 1158
AflIII ACRYGT 1 cut(s) 1033
AgeI ACCGGT 1 cut(s) 140
AgsI TTSAA 8 cut(s) 59, 329, 898, 927, 938, 1223, 1314, 1430
AjnI CCWGG 1 cut(s) 1006
AloI GAACNNNNNNTCC 2 cut(s) 969, 1001
AluBI AGCT 6 cut(s) 46, 73, 94, 239, 682, 1112
AluI AGCT 6 cut(s) 46, 73, 94, 239, 682, 1112
Alw21I GWGCWC 3 cut(s) 48, 96, 770
Alw26I GTCTC 4 cut(s) 326, 518, 1150, 1384
AlwI GGATC 4 cut(s) 278, 311, 760, 979
Aor13HI TCCGGA 1 cut(s) 866
AoxI GGCC 4 cut(s) 199, 573, 1000, 1160
ApeKI GCWGC 1 cut(s) 1040
ArsI GACNNNNNNTTYG 2 cut(s) 1338, 1370
AsiGI ACCGGT 1 cut(s) 140
Asp700I GAANNNNTTC 1 cut(s) 1227
AspLEI GCGC 1 cut(s) 415
AspS9I GGNCC 5 cut(s) 245, 573, 712, 968, 1001
AsuC2I CCSGG 3 cut(s) 243, 417, 958
AsuHPI GGTGA 5 cut(s) 404, 418, 1084, 1186, 1214
AvaII GGWCC 3 cut(s) 245, 712, 968
BaeGI GKGCMC 1 cut(s) 1198
BanI GGYRCC 1 cut(s) 412
BanII GRGCYC 3 cut(s) 48, 96, 1014
BbsI GAAGAC 2 cut(s) 132, 228
Bbv12I GWGCWC 3 cut(s) 48, 96, 770
BbvI GCAGC 1 cut(s) 1027
BccI CCATC 7 cut(s) 13, 19, 256, 454, 818, 864, 1184
BceAI ACGGC 2 cut(s) 214, 426
BcgI CGANNNNNNTGC 2 cut(s) 76, 110
BciT130I CCWGG 1 cut(s) 1008
BciVI GTATCC 2 cut(s) 874, 1049
BcnI CCSGG 3 cut(s) 243, 417, 958
BcoDI GTCTC 4 cut(s) 326, 518, 1150, 1384
BfaI CTAG 3 cut(s) 369, 450, 1080
BfoI RGCGCY 1 cut(s) 416
BfuI GTATCC 2 cut(s) 874, 1049
BisI GCNGC 2 cut(s) 302, 1041
BlsI GCNGC 2 cut(s) 303, 1042
Bme1390I CCNGG 4 cut(s) 243, 417, 958, 1008
Bme18I GGWCC 3 cut(s) 245, 712, 968
BmgT120I GGNCC 5 cut(s) 245, 573, 712, 968, 1001
BmiI GGNNCC 4 cut(s) 108, 145, 414, 525
BmrFI CCNGG 4 cut(s) 243, 417, 958, 1008
BmsI GCATC 2 cut(s) 1316, 1343
BpiI GAAGAC 2 cut(s) 132, 228
BpmI CTGGAG 2 cut(s) 528, 572
BpuEI CTTGAG 1 cut(s) 1141
BpuMI CCSGG 3 cut(s) 243, 417, 958
Bsa29I ATCGAT 2 cut(s) 315, 1090
BsaBI GATNNNNATC 1 cut(s) 1096
BsaHI GRCGYC 1 cut(s) 413
BsaI GGTCTC 2 cut(s) 518, 1384
BsaJI CCNNGG 2 cut(s) 378, 1007
BsaWI WCCGGW 3 cut(s) 140, 403, 866
Bsc4I CCNNNNNNNGG 4 cut(s) 422, 1013, 1066, 1158
Bse118I RCCGGY 1 cut(s) 140
Bse1I ACTGG 5 cut(s) 496, 555, 585, 626, 970
Bse3DI GCAATG 1 cut(s) 1372
Bse8I GATNNNNATC 1 cut(s) 1096
BseAI TCCGGA 1 cut(s) 866
BseBI CCWGG 1 cut(s) 1008
BseCI ATCGAT 2 cut(s) 315, 1090
BseDI CCNNGG 2 cut(s) 378, 1007
BseGI GGATG 2 cut(s) 875, 1334
BseJI GATNNNNATC 1 cut(s) 1096
BseLI CCNNNNNNNGG 4 cut(s) 422, 1013, 1066, 1158
BseMI GCAATG 1 cut(s) 1372
BseMII CTCAG 4 cut(s) 669, 700, 1155, 1161
BseNI ACTGG 5 cut(s) 496, 555, 585, 626, 970
BseRI GAGGAG 2 cut(s) 368, 479
BseSI GKGCMC 1 cut(s) 1198
BseXI GCAGC 1 cut(s) 1027
BshFI GGCC 4 cut(s) 201, 575, 1002, 1162
BshNI GGYRCC 1 cut(s) 412
BshTI ACCGGT 1 cut(s) 140
BshVI ATCGAT 2 cut(s) 315, 1090
BsiHKAI GWGCWC 3 cut(s) 48, 96, 770
BsiSI CCGG 7 cut(s) 104, 141, 243, 404, 416, 867, 958
BslI CCNNNNNNNGG 4 cut(s) 422, 1013, 1066, 1158
BsmAI GTCTC 4 cut(s) 326, 518, 1150, 1384
BsmI GAATGC 1 cut(s) 1081
BsnI GGCC 4 cut(s) 201, 575, 1002, 1162
Bso31I GGTCTC 2 cut(s) 518, 1384
Bsp1286I GDGCHC 5 cut(s) 48, 96, 770, 1014, 1198
Bsp13I TCCGGA 1 cut(s) 866
Bsp143I GATC 9 cut(s) 270, 312, 316, 343, 384, 752, 984, 1087, 1091
BspACI CCGC 5 cut(s) 101, 302, 971, 1217, 1324
BspANI GGCC 4 cut(s) 201, 575, 1002, 1162
BspCNI CTCAG 4 cut(s) 670, 699, 1154, 1160
BspDI ATCGAT 2 cut(s) 315, 1090
BspEI TCCGGA 1 cut(s) 866
BspHI TCATGA 1 cut(s) 910
BspLI GGNNCC 4 cut(s) 108, 145, 414, 525
BspPI GGATC 4 cut(s) 278, 311, 760, 979
BspT107I GGYRCC 1 cut(s) 412
BspTNI GGTCTC 2 cut(s) 518, 1384
BsrDI GCAATG 1 cut(s) 1372
BsrFI RCCGGY 1 cut(s) 140
BsrI ACTGG 5 cut(s) 496, 555, 585, 626, 970
BssAI RCCGGY 1 cut(s) 140
BssECI CCNNGG 2 cut(s) 378, 1007
BssMI GATC 9 cut(s) 270, 312, 316, 343, 384, 752, 984, 1087, 1091
BssNI GRCGYC 1 cut(s) 413
Bst2UI CCWGG 1 cut(s) 1008
Bst4CI ACNGT 3 cut(s) 159, 392, 1438
Bst6I CTCTTC 1 cut(s) 324
BstACI GRCGYC 1 cut(s) 413
BstC8I GCNNGC 1 cut(s) 582
BstDEI CTNAG 4 cut(s) 678, 686, 1141, 1147
BstF5I GGATG 2 cut(s) 875, 1334
BstH2I RGCGCY 1 cut(s) 416
BstHHI GCGC 1 cut(s) 415
BstKTI GATC 9 cut(s) 273, 315, 319, 346, 387, 755, 987, 1090, 1094
BstMAI GTCTC 4 cut(s) 326, 518, 1150, 1384
BstMBI GATC 9 cut(s) 270, 312, 316, 343, 384, 752, 984, 1087, 1091
BstMWI GCNNNNNNNGC 4 cut(s) 52, 100, 581, 1037
BstNI CCWGG 1 cut(s) 1008
BstNSI RCATGY 1 cut(s) 1037
BstSCI CCNGG 4 cut(s) 241, 415, 956, 1006
BstSLI GKGCMC 1 cut(s) 1198
BstV1I GCAGC 1 cut(s) 1027
BstV2I GAAGAC 2 cut(s) 132, 228
BstX2I RGATCY 1 cut(s) 984
BstYI RGATCY 1 cut(s) 984
Bsu15I ATCGAT 2 cut(s) 315, 1090
BsuI GTATCC 2 cut(s) 874, 1049
BsuRI GGCC 4 cut(s) 201, 575, 1002, 1162
BsuTUI ATCGAT 2 cut(s) 315, 1090
BtgZI GCGATG 1 cut(s) 705
BtsCI GGATG 2 cut(s) 875, 1334
BtsIMutI CAGTG 1 cut(s) 1443
Cac8I GCNNGC 1 cut(s) 582
CciI TCATGA 1 cut(s) 910
CfoI GCGC 1 cut(s) 415
Cfr10I RCCGGY 1 cut(s) 140
Cfr13I GGNCC 5 cut(s) 245, 573, 712, 968, 1001
ClaI ATCGAT 2 cut(s) 315, 1090
CseI GACGC 2 cut(s) 197, 458
CspAI ACCGGT 1 cut(s) 140
CspCI CAANNNNNGTGG 4 cut(s) 65, 100, 462, 497
CviAII CATG 9 cut(s) 7, 13, 31, 37, 485, 635, 911, 1034, 1418
DdeI CTNAG 4 cut(s) 678, 686, 1141, 1147
DinI GGCGCC 1 cut(s) 414
DpnI GATC 9 cut(s) 272, 314, 318, 345, 386, 754, 986, 1089, 1093
DpnII GATC 9 cut(s) 270, 312, 316, 343, 384, 752, 984, 1087, 1091
DraI TTTAAA 1 cut(s) 795
DraIII CACNNNGTG 1 cut(s) 1202
DrdI GACNNNNNNGTC 1 cut(s) 1398
DseDI GACNNNNNNGTC 1 cut(s) 1398
EaeI YGGCCR 1 cut(s) 199
Eam1104I CTCTTC 1 cut(s) 324
EarI CTCTTC 1 cut(s) 324
EciI GGCGGA 2 cut(s) 960, 1339
Ecl136II GAGCTC 2 cut(s) 46, 94
Eco24I GRGCYC 3 cut(s) 48, 96, 1014
Eco31I GGTCTC 2 cut(s) 518, 1384
Eco47I GGWCC 3 cut(s) 245, 712, 968
Eco53kI GAGCTC 2 cut(s) 46, 94
EcoICRI GAGCTC 2 cut(s) 46, 94
EcoO109I RGGNCCY 2 cut(s) 573, 712
EcoRII CCWGG 1 cut(s) 1006
EcoT38I GRGCYC 3 cut(s) 48, 96, 1014
EgeI GGCGCC 1 cut(s) 414
EheI GGCGCC 1 cut(s) 414
FaeI CATG 9 cut(s) 10, 16, 34, 40, 488, 638, 914, 1037, 1421
FalI AAGNNNNNCTT 2 cut(s) 974, 1006
FatI CATG 9 cut(s) 6, 12, 30, 36, 484, 634, 910, 1033, 1417
FauNDI CATATG 1 cut(s) 1361
FblI GTMKAC 3 cut(s) 161, 179, 962
Fnu4HI GCNGC 2 cut(s) 302, 1041
FokI GGATG 2 cut(s) 882, 1321
FriOI GRGCYC 3 cut(s) 48, 96, 1014
Fsp4HI GCNGC 2 cut(s) 302, 1041
FspBI CTAG 3 cut(s) 369, 450, 1080
GlaI GCGC 1 cut(s) 414
GluI GCNGC 2 cut(s) 302, 1041
GsuI CTGGAG 2 cut(s) 528, 572
HaeII RGCGCY 1 cut(s) 416
HaeIII GGCC 4 cut(s) 201, 575, 1002, 1162
HapII CCGG 7 cut(s) 104, 141, 243, 404, 416, 867, 958
HgaI GACGC 2 cut(s) 197, 458
HhaI GCGC 1 cut(s) 415
Hin1I GRCGYC 1 cut(s) 413
Hin1II CATG 9 cut(s) 10, 16, 34, 40, 488, 638, 914, 1037, 1421
Hin6I GCGC 1 cut(s) 413
HinP1I GCGC 1 cut(s) 413
HincII GTYRAC 1 cut(s) 162
HindII GTYRAC 1 cut(s) 162
HinfI GANTC 6 cut(s) 610, 737, 836, 907, 1154, 1441
HpaII CCGG 7 cut(s) 104, 141, 243, 404, 416, 867, 958
HphI GGTGA 5 cut(s) 404, 418, 1084, 1186, 1214
Hpy166II GTNNAC 4 cut(s) 162, 180, 473, 963
Hpy188III TCNNGA 9 cut(s) 116, 507, 591, 617, 707, 867, 911, 938, 1299
Hpy8I GTNNAC 4 cut(s) 162, 180, 473, 963
Hpy99I CGWCG 2 cut(s) 163, 392
HpyAV CCTTC 3 cut(s) 1264, 1392, 1405
HpyCH4III ACNGT 3 cut(s) 159, 392, 1438
HpyCH4IV ACGT 1 cut(s) 1399
HpyF10VI GCNNNNNNNGC 4 cut(s) 52, 100, 581, 1037
HpyF3I CTNAG 4 cut(s) 678, 686, 1141, 1147
HpySE526I ACGT 1 cut(s) 1399
Hsp92I GRCGYC 1 cut(s) 413
Hsp92II CATG 9 cut(s) 10, 16, 34, 40, 488, 638, 914, 1037, 1421
HspAI GCGC 1 cut(s) 413
KasI GGCGCC 1 cut(s) 412
Kpn2I TCCGGA 1 cut(s) 866
Kzo9I GATC 9 cut(s) 270, 312, 316, 343, 384, 752, 984, 1087, 1091
LmnI GCTCC 9 cut(s) 78, 91, 244, 264, 466, 553, 667, 765, 1109
Lsp1109I GCAGC 1 cut(s) 1027
LweI GCATC 2 cut(s) 1316, 1343
MaeI CTAG 3 cut(s) 369, 450, 1080
MaeII ACGT 1 cut(s) 1399
MaeIII GTNAC 5 cut(s) 293, 392, 406, 854, 1202
MalI GATC 9 cut(s) 272, 314, 318, 345, 386, 754, 986, 1089, 1093
MboI GATC 9 cut(s) 270, 312, 316, 343, 384, 752, 984, 1087, 1091
MflI RGATCY 1 cut(s) 984
MhlI GDGCHC 5 cut(s) 48, 96, 770, 1014, 1198
MluCI AATT 4 cut(s) 50, 885, 922, 952
Mly113I GGCGCC 1 cut(s) 413
MlyI GAGTC 2 cut(s) 845, 1148
MroI TCCGGA 1 cut(s) 866
MroXI GAANNNNTTC 1 cut(s) 1227
MseI TTAA 4 cut(s) 794, 888, 1281, 1343
MslI CAYNNNNRTG 5 cut(s) 11, 17, 35, 404, 897
MspI CCGG 7 cut(s) 104, 141, 243, 404, 416, 867, 958
MspR9I CCNGG 4 cut(s) 243, 417, 958, 1008
Mva1269I GAATGC 1 cut(s) 1081
MvaI CCWGG 1 cut(s) 1008
MwoI GCNNNNNNNGC 4 cut(s) 52, 100, 581, 1037
NarI GGCGCC 1 cut(s) 413
NciI CCSGG 3 cut(s) 243, 417, 958
NdeI CATATG 1 cut(s) 1361
NdeII GATC 9 cut(s) 270, 312, 316, 343, 384, 752, 984, 1087, 1091
NlaIII CATG 9 cut(s) 10, 16, 34, 40, 488, 638, 914, 1037, 1421
NlaIV GGNNCC 4 cut(s) 108, 145, 414, 525
NmuCI GTSAC 3 cut(s) 392, 406, 1202
NspI RCATGY 1 cut(s) 1037
PagI TCATGA 1 cut(s) 910
PciI ACATGT 1 cut(s) 1033
PcsI WCGNNNNNNNCGW 2 cut(s) 158, 231
PctI GAATGC 1 cut(s) 1081
PdmI GAANNNNTTC 1 cut(s) 1227
PfeI GAWTC 4 cut(s) 610, 737, 907, 1441
PfoI TCCNGGA 1 cut(s) 241
PinAI ACCGGT 1 cut(s) 140
PkrI GCNGC 2 cut(s) 303, 1042
PleI GAGTC 2 cut(s) 844, 1148
PluTI GGCGCC 1 cut(s) 416
PpsI GAGTC 2 cut(s) 844, 1148
PpuMI RGGWCCY 1 cut(s) 712
PscI ACATGT 1 cut(s) 1033
Psp124BI GAGCTC 2 cut(s) 48, 96
Psp5II RGGWCCY 1 cut(s) 712
Psp6I CCWGG 1 cut(s) 1006
PspGI CCWGG 1 cut(s) 1006
PspN4I GGNNCC 4 cut(s) 108, 145, 414, 525
PspPI GGNCC 5 cut(s) 245, 573, 712, 968, 1001
PspPPI RGGWCCY 1 cut(s) 712
PsuI RGATCY 1 cut(s) 984
RseI CAYNNNNRTG 5 cut(s) 11, 17, 35, 404, 897
SacI GAGCTC 2 cut(s) 48, 96
SalI GTCGAC 1 cut(s) 160
SaqAI TTAA 4 cut(s) 794, 888, 1281, 1343
SatI GCNGC 2 cut(s) 302, 1041
Sau3AI GATC 9 cut(s) 270, 312, 316, 343, 384, 752, 984, 1087, 1091
Sau96I GGNCC 5 cut(s) 245, 573, 712, 968, 1001
SchI GAGTC 2 cut(s) 845, 1148
ScrFI CCNGG 4 cut(s) 243, 417, 958, 1008
SduI GDGCHC 5 cut(s) 48, 96, 770, 1014, 1198
SfaNI GCATC 2 cut(s) 1316, 1343
SfoI GGCGCC 1 cut(s) 414
SinI GGWCC 3 cut(s) 245, 712, 968
SmiMI CAYNNNNRTG 5 cut(s) 11, 17, 35, 404, 897
SmlI CTYRAG 1 cut(s) 1156
SmoI CTYRAG 1 cut(s) 1156
Sse9I AATT 4 cut(s) 50, 885, 922, 952
SsiI CCGC 5 cut(s) 101, 302, 971, 1217, 1324
SspDI GGCGCC 1 cut(s) 412
SspMI CTAG 3 cut(s) 369, 450, 1080
SstI GAGCTC 2 cut(s) 48, 96
StyD4I CCNGG 4 cut(s) 241, 415, 956, 1006
TaaI ACNGT 3 cut(s) 159, 392, 1438
TaiI ACGT 1 cut(s) 1402
TaqI TCGA 8 cut(s) 48, 96, 161, 273, 315, 387, 1090, 1444
TasI AATT 4 cut(s) 50, 885, 922, 952
TauI GCSGC 1 cut(s) 304
TfiI GAWTC 4 cut(s) 610, 737, 907, 1441
Tru1I TTAA 4 cut(s) 794, 888, 1281, 1343
Tru9I TTAA 4 cut(s) 794, 888, 1281, 1343
TscAI CASTG 1 cut(s) 1443
TseFI GTSAC 3 cut(s) 392, 406, 1202
TseI GCWGC 1 cut(s) 1040
Tsp45I GTSAC 3 cut(s) 392, 406, 1202
TspDTI ATGAA 8 cut(s) 388, 651, 708, 894, 920, 927, 1151, 1406
TspGWI ACGGA 3 cut(s) 104, 277, 1221
TspRI CASTG 1 cut(s) 1443
VpaK11BI GGWCC 3 cut(s) 245, 712, 968
XceI RCATGY 1 cut(s) 1037
XmiI GTMKAC 3 cut(s) 161, 179, 962
XmnI GAANNNNTTC 1 cut(s) 1227
XspI CTAG 3 cut(s) 369, 450, 1080
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.