Rroxscaffold_5G00333110

chromosome transmission fidelity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0000880
Physical Location & Seq
Forward (+)
537642 .. 549379
11738 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00333110.1

Sequence Viewer

Length: 3003 bp
ATGTGTTCGAACCTCGATCTGAGAGAAGAGGCCGCAGTAGCCGCTGGATTTGAGGTGATCGACGGCGTCGTTGACTCGTTGGGATTGCCGCAGAATCGTAGATCTACCAAAGCAGCGGAAGAGGGATGGAGCGAGACAATGGACATGGACATGGACGAGCTCGAATTGCTTGAAGCCAACTATCAGCTCCACGAAGATTGCACGGAGCTCGAACCGCCAGAACCCTATCCAGAAGAAGAAGACCAACATCCTACCGGTTCCAAATCGCCGCCGTCGACTGACCACCAGCAGTCTACTGAAGCCCAAATCAACGGCCACAAGCGTTTACGACCAGAAGAAGAAACTGCCGAAGCTCCCGGACCATCAGACGAGAAACGGAGCAGGATCGAAGATGAGGATTGGCTTCGATACTCGCCGCCACACCAGATCGATCCTGTTGTTGAAGAAACAAGTGCTGATCTTGTGGAGGAGAAGATTGTGTATAGGTATGCCTCCGAGATCGACGGTGATTTCATTCCGGTGACGGCGCCGGGTTTGGGCGATAGGGTTTATGCCAAGATTTCTAGGGTGGAGATGGAGGAGCGTCCACAGAGATTGCATGGAACTGGACAATCTCAATCTGGAGGTCTCATTTCGGAACCTATTGATGTTCTACTGCAAAGACTGGAGCAAGAGGCATTTACAAGGGCCTTGCAAGCCAGTTCCAGAGGTCAAAGTGATGTGATTCTTCCCGAAACACCAGTTGTGCATGAACGACTTTGGGTGGATAAATATGCTCCAAACTCATTTACTGAGCTTCTTAGCGATGAACAGACAAATCGTGAGGTCCTGCTTTGGCTAAAACAATGGGATTCTTGTGTTTTTGGATCAGAGATTAGGAGCACATCAGATGAGGTTTTGTCTGCTTTAAAACGACATTCTTCTATCACTCAACATCAAAAACTTTCCGAGTCAAAGTTTCCAAGAAGTAACAGAAGATCCAGATGGAATAATGAAAAAATTAAGCATTTCGATGGTATGAATCATGAAAATACTAATTCAAAAAGCGTTCAAGAGTTGTCAAACAATTCCCGGTCTACTGGTCCGCCAGAACAAAAGATCCTCTTACTTTGTGGCCCACCAGGGCTCGGAAAGACTACACTTGCACATGTGGCTGCAAAGCATTGTGGATACCGAGTGGTGGAGGTGAATGCTAGTGATGATCGATCATCATCAACTATAGGAGCTAAAATTCTTGATGTTGTTCAGATGAACTCAGTCTCAGCCGACTCAAGGCCAAAGTGTTTGGTGATTGACGAAATAGATGGGGCACTTGGTGACGGAAAAGGTGCGGTTGAAGTCATTCTAAAAATGGTTTCTGCTGATAAGAAGTCTGATATGGAGAAGGAAAATGTTAATAAAGAACAAACATCAGGAAAGGCATCTTCAAAAAAGGGGCGGAAATCTGCATCCTTGTTAAGACCTATTATTTGCATATGCAATGACCTATACGCACCTTCGTTGAGACCATTACGTCAGATAGCGAAGGTTCATGTTTTTGTTCAACCAACAGTGAATCGAGTGGTGAGCAGGCTTAAATATATATGTAACAAGGAAGGAATGAAAACAAGTTCCATTGCACTTACTGTACTTGCAGAGCATACAGAATGCGATATACGTTCATGCTTGAACACTCTTCAGTTCCTGAACAAGAAGAAGGAAGCACTCAACGCTTGGGATATTGGCTCTCAAGTGGTTGGTCGAAAGGATATGTCAAGAGGTGTGTTTGATGTCTGGAAAGAGATCTTCCAAAAGAGAAAAGCGAAGAGGCAGCAAAGGACAAATAACAATTGTGGAATAATATCAAGTGAATTCGACTCTTTGCATTCTCTCATTTCTCACCGTGGTGACTATGATTTGATTGTAGATGGTCTTCATGAAAACGTTTTGCATCTTCCTTATCATGATCCAGTGATGCAAAAGACTGTCAAATGCTTGAACAGCCTTGGTGTCTCTGACCTTATGAACAAATATATTATGCGTACACAACACATGTCCCTTTATGCTTATCAGCCTGCTATTGCCATAATTGTACATCGTTTAGTAGCCCAAGTGCAGAGACCGAATATTGAGTGGCCAAAATCTTATTATAGGTACCGGACAATGTTAATGGAAAAAGTGGACAGTTTGAAGTCTTGGTACAACACAATTCCACCATCTATTTCAAGGCATTTGTCAATTAAGTCCTTTGTAGAAGATTCAATTTCATTGCTGTTACACATCCTATCACCCCCAACTCTAAGGCTGGCCGCATTGCACTTATTATCAGAGAAGGAGAAGAATGATCTGGCTCAGTTAGTTAGCACGATGGTCTCCTATTCTATAACACATAGAAGGATCAAAGCTGATCCTGTGACTGGCAATCTAGGGCATCGAGCAGCTGATGTGTCGGAGCTGTCTTTTGATCCCCCCATTAATGGCTTTGTTAACTTCCAGGGCTACAGATCTGGCCATCATGTACTTGCCTCAACTATGAAACAGATGTTGGCGCACGAGGTTGAAAAGCAAAGGATTTTGCAAGCAAGCATTGGGATATCTGCACAGTTGACGGATGGATACAACAAGGAAAACCATGCTTCTTTAAGGCTAGAAACTAGGAGATTACAATCTGGTAGAGCTGATCATTTAGGTGCATCTGCTGTGAACAATGGAAAGTCAGAAAGCATGTCAAATAGGACACAATCAAATCCCAGTATTTCCGGTGTTTTACCTAACTTGGGCACTGGGAATACCTCAGTGAGTACAAAGTTAAACTCCTCTGGATGTATGAGAAAGCCGTCTGGTGGTTCCTCTAGTTTCTTTGATAGGTTTAGAAAGCTTGGTAAAGGTTCTCAGAACACTGCTGAGGCTGTGCGGAAGGAAGCAACCTCTGAGAGAGATTCACGTCCTTTGGTCTTTAAATTTAATGAGGGCTTTACAAATGCAGTTAAAAGGCCAGTTCGTGTTCGGGAGTTTCTGACGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AAA

Protein Analysis

1000

Amino Acids

111.93

Weight (kDa)

7.2

Isoelectric Point (pI)

53.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AAA PF00004 368 - 452 1.5e-13 ATPase family associated with various cellular activities (AAA)
AAA_lid_RFC1 PF25361 519 - 570 7e-08 RFC1-like, AAA+ ATPase lid domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1512
Acc65I GGTACC 1 cut(s) 2133
AccB1I GGYRCC 2 cut(s) 526, 2133
AccI GTMKAC 3 cut(s) 275, 293, 1076
AclI AACGTT 1 cut(s) 1923
AclWI GGATC 9 cut(s) 392, 425, 874, 972, 1093, 1940, 2381, 2384, 2438
AcoI YGGCCR 4 cut(s) 313, 2114, 2286, 2488
AcsI RAATTY 3 cut(s) 1230, 1850, 2939
AcuI CTGAAG 2 cut(s) 318, 1661
AcyI GRCGYC 2 cut(s) 66, 527
AdeI CACNNNGTG 1 cut(s) 1316
AfaI GTAC 7 cut(s) 1629, 2023, 2073, 2135, 2180, 2499, 2782
AfiI CCNNNNNNNGG 8 cut(s) 536, 1127, 1180, 1272, 2396, 2456, 2756, 2822
AflIII ACRYGT 2 cut(s) 1147, 2031
AgeI ACCGGT 1 cut(s) 254
AjiI CACGTC 1 cut(s) 2924
AjnI CCWGG 2 cut(s) 1120, 2472
AjuI GAANNNNNNNTTGG 2 cut(s) 2507, 2539
AleI CACNNNNGTG 1 cut(s) 1884
AloI GAACNNNNNNTCC 2 cut(s) 1083, 1115
Alw21I GWGCWC 3 cut(s) 162, 210, 884
Alw26I GTCTC 7 cut(s) 128, 632, 1264, 1498, 1996, 2092, 2356
AlwI GGATC 9 cut(s) 392, 425, 874, 972, 1093, 1940, 2381, 2384, 2438
AlwNI CAGNNNCTG 1 cut(s) 1684
AoxI GGCC 9 cut(s) 30, 313, 687, 1114, 1274, 2114, 2286, 2488, 2972
ApeKI GCWGC 4 cut(s) 113, 1154, 1810, 2417
ApoI RAATTY 3 cut(s) 1230, 1850, 2939
ArsI GACNNNNNNTTYG 2 cut(s) 1452, 1484
AseI ATTAAT 1 cut(s) 2454
AsiGI ACCGGT 1 cut(s) 254
Asp700I GAANNNNTTC 1 cut(s) 1341
Asp718I GGTACC 1 cut(s) 2133
AspLEI GCGC 2 cut(s) 529, 2530
AspS9I GGNCC 5 cut(s) 359, 687, 826, 1082, 1115
AsuC2I CCSGG 3 cut(s) 357, 531, 1072
AsuII TTCGAA 1 cut(s) 8
AvaII GGWCC 3 cut(s) 359, 826, 1082
BaeGI GKGCMC 2 cut(s) 1312, 2762
BalI TGGCCA 2 cut(s) 2116, 2490
BanI GGYRCC 2 cut(s) 526, 2133
BanII GRGCYC 3 cut(s) 162, 210, 1128
BarI GAAGNNNNNNTAC 4 cut(s) 1896, 1928, 2162, 2194
BauI CACGAG 1 cut(s) 2531
BbsI GAAGAC 2 cut(s) 246, 1904
Bbv12I GWGCWC 3 cut(s) 162, 210, 884
BbvCI CCTCAGC 1 cut(s) 2883
BbvI GCAGC 4 cut(s) 125, 1141, 1822, 2429
BceAI ACGGC 5 cut(s) 79, 256, 328, 540, 2800
BcgI CGANNNNNNTGC 2 cut(s) 190, 224
BciT130I CCWGG 2 cut(s) 1122, 2474
BciVI GTATCC 2 cut(s) 1163, 2588
BclI TGATCA 1 cut(s) 2659
BcnI CCSGG 3 cut(s) 357, 531, 1072
BcoDI GTCTC 7 cut(s) 128, 632, 1264, 1498, 1996, 2092, 2356
BfaI CTAG 6 cut(s) 564, 1194, 2405, 2627, 2634, 2832
BfmI CTRYAG 2 cut(s) 1218, 2479
BfoI RGCGCY 1 cut(s) 530
BfuI GTATCC 2 cut(s) 1163, 2588
BglII AGATCT 3 cut(s) 101, 1782, 2483
Bme1390I CCNGG 5 cut(s) 357, 531, 1072, 1122, 2474
Bme18I GGWCC 3 cut(s) 359, 826, 1082
BmgBI CACGTC 1 cut(s) 2924
BmgT120I GGNCC 5 cut(s) 359, 687, 826, 1082, 1115
BmiI GGNNCC 5 cut(s) 259, 528, 639, 2135, 2827
BmrFI CCNGG 5 cut(s) 357, 531, 1072, 1122, 2474
BmrI ACTGGG 2 cut(s) 2724, 2772
BmsI GCATC 6 cut(s) 1430, 1457, 1939, 1944, 2419, 2681
BmuI ACTGGG 2 cut(s) 2724, 2772
BpiI GAAGAC 2 cut(s) 246, 1904
BpmI CTGGAG 2 cut(s) 642, 686
Bpu10I CCTNAGC 1 cut(s) 2883
Bpu14I TTCGAA 1 cut(s) 8
BpuEI CTTGAG 2 cut(s) 1255, 1713
BpuMI CCSGG 3 cut(s) 357, 531, 1072
Bsa29I ATCGAT 2 cut(s) 429, 1204
BsaBI GATNNNNATC 2 cut(s) 1210, 2644
BsaHI GRCGYC 2 cut(s) 66, 527
BsaI GGTCTC 4 cut(s) 632, 1498, 2092, 2356
BsaJI CCNNGG 4 cut(s) 1121, 1882, 1984, 2473
BsaWI WCCGGW 4 cut(s) 254, 517, 2136, 2738
Bsc4I CCNNNNNNNGG 8 cut(s) 536, 1127, 1180, 1272, 2396, 2456, 2756, 2822
Bse118I RCCGGY 1 cut(s) 254
Bse3DI GCAATG 4 cut(s) 1486, 1614, 2246, 2291
Bse8I GATNNNNATC 2 cut(s) 1210, 2644
BseBI CCWGG 2 cut(s) 1122, 2474
BseCI ATCGAT 2 cut(s) 429, 1204
BseDI CCNNGG 4 cut(s) 1121, 1882, 1984, 2473
BseGI GGATG 6 cut(s) 131, 247, 1448, 2259, 2596, 2807
BseJI GATNNNNATC 2 cut(s) 1210, 2644
BseLI CCNNNNNNNGG 8 cut(s) 536, 1127, 1180, 1272, 2396, 2456, 2756, 2822
BseMI GCAATG 4 cut(s) 1486, 1614, 2246, 2291
BseMII CTCAG 9 cut(s) 11, 783, 1269, 1275, 2345, 2787, 2874, 2885, 2901
BseRI GAGGAG 3 cut(s) 482, 593, 2785
BseSI GKGCMC 2 cut(s) 1312, 2762
BseXI GCAGC 4 cut(s) 125, 1141, 1822, 2429
BsgI GTGCAG 2 cut(s) 2114, 2562
BshFI GGCC 9 cut(s) 32, 315, 689, 1116, 1276, 2116, 2288, 2490, 2974
BshNI GGYRCC 2 cut(s) 526, 2133
BshTI ACCGGT 1 cut(s) 254
BshVI ATCGAT 2 cut(s) 429, 1204
BsiHKAI GWGCWC 3 cut(s) 162, 210, 884
BsiSI CCGG 7 cut(s) 255, 357, 518, 530, 1072, 2137, 2739
BslFI GGGAC 1 cut(s) 2020
BslI CCNNNNNNNGG 8 cut(s) 536, 1127, 1180, 1272, 2396, 2456, 2756, 2822
BsmAI GTCTC 7 cut(s) 128, 632, 1264, 1498, 1996, 2092, 2356
BsmFI GGGAC 1 cut(s) 2020
BsmI GAATGC 3 cut(s) 1195, 1652, 1864
BsnI GGCC 9 cut(s) 32, 315, 689, 1116, 1276, 2116, 2288, 2490, 2974
Bso31I GGTCTC 4 cut(s) 632, 1498, 2092, 2356
Bsp119I TTCGAA 1 cut(s) 8
Bsp1286I GDGCHC 6 cut(s) 162, 210, 884, 1128, 1312, 2762
Bsp1407I TGTACA 1 cut(s) 2071
BspANI GGCC 9 cut(s) 32, 315, 689, 1116, 1276, 2116, 2288, 2490, 2974
BspCNI CTCAG 9 cut(s) 12, 784, 1268, 1274, 2344, 2786, 2875, 2884, 2902
BspDI ATCGAT 2 cut(s) 429, 1204
BspHI TCATGA 3 cut(s) 1024, 1915, 1942
BspLI GGNNCC 5 cut(s) 259, 528, 639, 2135, 2827
BspPI GGATC 9 cut(s) 392, 425, 874, 972, 1093, 1940, 2381, 2384, 2438
BspT104I TTCGAA 1 cut(s) 8
BspT107I GGYRCC 2 cut(s) 526, 2133
BspTNI GGTCTC 4 cut(s) 632, 1498, 2092, 2356
BsrDI GCAATG 4 cut(s) 1486, 1614, 2246, 2291
BsrFI RCCGGY 1 cut(s) 254
BsrGI TGTACA 1 cut(s) 2071
BssAI RCCGGY 1 cut(s) 254
BssECI CCNNGG 4 cut(s) 1121, 1882, 1984, 2473
BssNI GRCGYC 2 cut(s) 66, 527
BssSI CACGAG 1 cut(s) 2531
BssT1I CCWWGG 1 cut(s) 1984
Bst2BI CACGAG 1 cut(s) 2531
Bst2UI CCWGG 2 cut(s) 1122, 2474
Bst4CI ACNGT 7 cut(s) 506, 1552, 1627, 1883, 1966, 2165, 2583
Bst6I CTCTTC 4 cut(s) 21, 114, 1680, 1799
BstACI GRCGYC 2 cut(s) 66, 527
BstAUI TGTACA 1 cut(s) 2071
BstBI TTCGAA 1 cut(s) 8
BstC8I GCNNGC 6 cut(s) 696, 1571, 2055, 2286, 2559, 2563
BstDSI CCRYGG 1 cut(s) 1882
BstF5I GGATG 6 cut(s) 131, 247, 1448, 2259, 2596, 2807
BstH2I RGCGCY 1 cut(s) 530
BstHHI GCGC 2 cut(s) 529, 2530
BstMAI GTCTC 7 cut(s) 128, 632, 1264, 1498, 1996, 2092, 2356
BstMWI GCNNNNNNNGC 8 cut(s) 38, 41, 166, 214, 695, 1151, 1709, 1980
BstNI CCWGG 2 cut(s) 1122, 2474
BstNSI RCATGY 3 cut(s) 1151, 2035, 2707
BstSCI CCNGG 5 cut(s) 355, 529, 1070, 1120, 2472
BstSFI CTRYAG 2 cut(s) 1218, 2479
BstSLI GKGCMC 2 cut(s) 1312, 2762
BstV1I GCAGC 4 cut(s) 125, 1141, 1822, 2429
BstV2I GAAGAC 2 cut(s) 246, 1904
BstX2I RGATCY 5 cut(s) 101, 977, 1098, 1782, 2483
BstYI RGATCY 5 cut(s) 101, 977, 1098, 1782, 2483
Bsu15I ATCGAT 2 cut(s) 429, 1204
BsuI GTATCC 2 cut(s) 1163, 2588
BsuRI GGCC 9 cut(s) 32, 315, 689, 1116, 1276, 2116, 2288, 2490, 2974
BsuTUI ATCGAT 2 cut(s) 429, 1204
BtgI CCRYGG 1 cut(s) 1882
BtgZI GCGATG 1 cut(s) 819
BtrI CACGTC 1 cut(s) 2924
BtsCI GGATG 6 cut(s) 131, 247, 1448, 2259, 2596, 2807
BtsI GCAGTG 1 cut(s) 2877
BtsIMutI CAGTG 5 cut(s) 1557, 1956, 2760, 2781, 2877
Cac8I GCNNGC 6 cut(s) 696, 1571, 2055, 2286, 2559, 2563
CaiI CAGNNNCTG 1 cut(s) 1684
CciI TCATGA 3 cut(s) 1024, 1915, 1942
CfoI GCGC 2 cut(s) 529, 2530
Cfr10I RCCGGY 1 cut(s) 254
Cfr13I GGNCC 5 cut(s) 359, 687, 826, 1082, 1115
ClaI ATCGAT 2 cut(s) 429, 1204
CseI GACGC 2 cut(s) 55, 572
Csp6I GTAC 7 cut(s) 1628, 2022, 2072, 2134, 2179, 2498, 2781
CspAI ACCGGT 1 cut(s) 254
CspCI CAANNNNNGTGG 4 cut(s) 179, 214, 576, 611
CviQI GTAC 7 cut(s) 1628, 2022, 2072, 2134, 2179, 2498, 2781
DinI GGCGCC 1 cut(s) 528
DraI TTTAAA 2 cut(s) 909, 2938
DraIII CACNNNGTG 1 cut(s) 1316
DrdI GACNNNNNNGTC 1 cut(s) 1512
DseDI GACNNNNNNGTC 1 cut(s) 1512
EaeI YGGCCR 4 cut(s) 313, 2114, 2286, 2488
Eam1104I CTCTTC 4 cut(s) 21, 114, 1680, 1799
EarI CTCTTC 4 cut(s) 21, 114, 1680, 1799
EciI GGCGGA 2 cut(s) 1074, 1453
Ecl136II GAGCTC 2 cut(s) 160, 208
Eco130I CCWWGG 1 cut(s) 1984
Eco24I GRGCYC 3 cut(s) 162, 210, 1128
Eco31I GGTCTC 4 cut(s) 632, 1498, 2092, 2356
Eco32I GATATC 1 cut(s) 2574
Eco47I GGWCC 3 cut(s) 359, 826, 1082
Eco53kI GAGCTC 2 cut(s) 160, 208
Eco57I CTGAAG 2 cut(s) 318, 1661
EcoICRI GAGCTC 2 cut(s) 160, 208
EcoO109I RGGNCCY 2 cut(s) 687, 826
EcoRI GAATTC 1 cut(s) 1850
EcoRII CCWGG 2 cut(s) 1120, 2472
EcoRV GATATC 1 cut(s) 2574
EcoT14I CCWWGG 1 cut(s) 1984
EcoT38I GRGCYC 3 cut(s) 162, 210, 1128
EgeI GGCGCC 1 cut(s) 528
EheI GGCGCC 1 cut(s) 528
ErhI CCWWGG 1 cut(s) 1984
FalI AAGNNNNNCTT 2 cut(s) 1088, 1120
FaqI GGGAC 1 cut(s) 2020
FauNDI CATATG 1 cut(s) 1475
FbaI TGATCA 1 cut(s) 2659
FblI GTMKAC 3 cut(s) 275, 293, 1076
FokI GGATG 6 cut(s) 138, 234, 1435, 2246, 2603, 2814
FriOI GRGCYC 3 cut(s) 162, 210, 1128
FspBI CTAG 6 cut(s) 564, 1194, 2405, 2627, 2634, 2832
GlaI GCGC 2 cut(s) 528, 2529
GsuI CTGGAG 2 cut(s) 642, 686
HaeII RGCGCY 1 cut(s) 530
HaeIII GGCC 9 cut(s) 32, 315, 689, 1116, 1276, 2116, 2288, 2490, 2974
HapII CCGG 7 cut(s) 255, 357, 518, 530, 1072, 2137, 2739
HgaI GACGC 2 cut(s) 55, 572
HhaI GCGC 2 cut(s) 529, 2530
Hin1I GRCGYC 2 cut(s) 66, 527
Hin6I GCGC 2 cut(s) 527, 2528
HinP1I GCGC 2 cut(s) 527, 2528
HincII GTYRAC 4 cut(s) 73, 276, 2467, 2586
HindII GTYRAC 4 cut(s) 73, 276, 2467, 2586
HindIII AAGCTT 1 cut(s) 2855
HpaI GTTAAC 1 cut(s) 2467
HpaII CCGG 7 cut(s) 255, 357, 518, 530, 1072, 2137, 2739
Hpy99I CGWCG 4 cut(s) 65, 71, 277, 506
HpyAV CCTTC 8 cut(s) 1378, 1506, 1519, 1589, 1690, 2305, 2367, 2890
HpyCH4III ACNGT 7 cut(s) 506, 1552, 1627, 1883, 1966, 2165, 2583
HpyCH4IV ACGT 5 cut(s) 1513, 1657, 1923, 2923, 2999
HpyF10VI GCNNNNNNNGC 8 cut(s) 38, 41, 166, 214, 695, 1151, 1709, 1980
HpySE526I ACGT 5 cut(s) 1513, 1657, 1923, 2923, 2999
Hsp92I GRCGYC 2 cut(s) 66, 527
HspAI GCGC 2 cut(s) 527, 2528
KasI GGCGCC 1 cut(s) 526
KpnI GGTACC 1 cut(s) 2137
Ksp22I TGATCA 1 cut(s) 2659
KspAI GTTAAC 1 cut(s) 2467
Lsp1109I GCAGC 4 cut(s) 125, 1141, 1822, 2429
LweI GCATC 6 cut(s) 1430, 1457, 1939, 1944, 2419, 2681
MaeI CTAG 6 cut(s) 564, 1194, 2405, 2627, 2634, 2832
MaeII ACGT 5 cut(s) 1513, 1657, 1923, 2923, 2999
MaeIII GTNAC 7 cut(s) 520, 968, 1316, 1586, 1886, 2253, 2392
MfeI CAATTG 1 cut(s) 1828
MflI RGATCY 5 cut(s) 101, 977, 1098, 1782, 2483
MhlI GDGCHC 6 cut(s) 162, 210, 884, 1128, 1312, 2762
MlsI TGGCCA 2 cut(s) 2116, 2490
MluNI TGGCCA 2 cut(s) 2116, 2490
Mly113I GGCGCC 1 cut(s) 527
MlyI GAGTC 4 cut(s) 68, 959, 1262, 1850
MmeI TCCRAC 1 cut(s) 2409
Mox20I TGGCCA 2 cut(s) 2116, 2490
MroXI GAANNNNTTC 1 cut(s) 1341
MscI TGGCCA 2 cut(s) 2116, 2490
MslI CAYNNNNRTG 5 cut(s) 149, 518, 1011, 1884, 2667
Msp20I TGGCCA 2 cut(s) 2116, 2490
MspA1I CMGCKG 3 cut(s) 44, 116, 2420
MspI CCGG 7 cut(s) 255, 357, 518, 530, 1072, 2137, 2739
MspR9I CCNGG 5 cut(s) 357, 531, 1072, 1122, 2474
MunI CAATTG 1 cut(s) 1828
Mva1269I GAATGC 3 cut(s) 1195, 1652, 1864
MvaI CCWGG 2 cut(s) 1122, 2474
MwoI GCNNNNNNNGC 8 cut(s) 38, 41, 166, 214, 695, 1151, 1709, 1980
NarI GGCGCC 1 cut(s) 527
NciI CCSGG 3 cut(s) 357, 531, 1072
NdeI CATATG 1 cut(s) 1475
NlaIV GGNNCC 5 cut(s) 259, 528, 639, 2135, 2827
NmuCI GTSAC 4 cut(s) 520, 1316, 1886, 2392
NspI RCATGY 3 cut(s) 1151, 2035, 2707
NspV TTCGAA 1 cut(s) 8
OliI CACNNNNGTG 1 cut(s) 1884
PagI TCATGA 3 cut(s) 1024, 1915, 1942
PciI ACATGT 2 cut(s) 1147, 2031
PcsI WCGNNNNNNNCGW 2 cut(s) 66, 272
PctI GAATGC 3 cut(s) 1195, 1652, 1864
PdmI GAANNNNTTC 1 cut(s) 1341
PfeI GAWTC 7 cut(s) 94, 724, 851, 1021, 1555, 2237, 2918
PflFI GACNNNGTC 1 cut(s) 65
PfoI TCCNGGA 1 cut(s) 355
PinAI ACCGGT 1 cut(s) 254
PleI GAGTC 4 cut(s) 68, 958, 1262, 1850
PluTI GGCGCC 1 cut(s) 530
PpsI GAGTC 4 cut(s) 68, 958, 1262, 1850
PpuMI RGGWCCY 1 cut(s) 826
PscI ACATGT 2 cut(s) 1147, 2031
PshBI ATTAAT 1 cut(s) 2454
Psp124BI GAGCTC 2 cut(s) 162, 210
Psp1406I AACGTT 1 cut(s) 1923
Psp5II RGGWCCY 1 cut(s) 826
Psp6I CCWGG 2 cut(s) 1120, 2472
PspGI CCWGG 2 cut(s) 1120, 2472
PspN4I GGNNCC 5 cut(s) 259, 528, 639, 2135, 2827
PspPI GGNCC 5 cut(s) 359, 687, 826, 1082, 1115
PspPPI RGGWCCY 1 cut(s) 826
PstNI CAGNNNCTG 1 cut(s) 1684
PsuI RGATCY 5 cut(s) 101, 977, 1098, 1782, 2483
PsyI GACNNNGTC 1 cut(s) 65
PvuII CAGCTG 1 cut(s) 2420
RsaI GTAC 7 cut(s) 1629, 2023, 2073, 2135, 2180, 2499, 2782
RsaNI GTAC 7 cut(s) 1628, 2022, 2072, 2134, 2179, 2498, 2781
RseI CAYNNNNRTG 5 cut(s) 149, 518, 1011, 1884, 2667
SacI GAGCTC 2 cut(s) 162, 210
SalI GTCGAC 1 cut(s) 274
Sau96I GGNCC 5 cut(s) 359, 687, 826, 1082, 1115
SchI GAGTC 4 cut(s) 68, 959, 1262, 1850
ScrFI CCNGG 5 cut(s) 357, 531, 1072, 1122, 2474
SduI GDGCHC 6 cut(s) 162, 210, 884, 1128, 1312, 2762
SfaNI GCATC 6 cut(s) 1430, 1457, 1939, 1944, 2419, 2681
SfcI CTRYAG 2 cut(s) 1218, 2479
SfoI GGCGCC 1 cut(s) 528
SfuI TTCGAA 1 cut(s) 8
SinI GGWCC 3 cut(s) 359, 826, 1082
SmiMI CAYNNNNRTG 5 cut(s) 149, 518, 1011, 1884, 2667
SmlI CTYRAG 2 cut(s) 1270, 1728
SmoI CTYRAG 2 cut(s) 1270, 1728
SspDI GGCGCC 1 cut(s) 526
SspI AATATT 1 cut(s) 2107
SspMI CTAG 6 cut(s) 564, 1194, 2405, 2627, 2634, 2832
SstI GAGCTC 2 cut(s) 162, 210
StyD4I CCNGG 5 cut(s) 355, 529, 1070, 1120, 2472
StyI CCWWGG 1 cut(s) 1984
TaaI ACNGT 7 cut(s) 506, 1552, 1627, 1883, 1966, 2165, 2583
TaiI ACGT 5 cut(s) 1516, 1660, 1926, 2926, 3002
TaqII GACCGA 1 cut(s) 2116
TatI WGTACW 4 cut(s) 1627, 2071, 2497, 2780
TauI GCSGC 6 cut(s) 35, 44, 91, 271, 418, 2291
TfiI GAWTC 7 cut(s) 94, 724, 851, 1021, 1555, 2237, 2918
TscAI CASTG 5 cut(s) 1557, 1956, 2767, 2781, 2884
TseFI GTSAC 4 cut(s) 520, 1316, 1886, 2392
TseI GCWGC 4 cut(s) 113, 1154, 1810, 2417
Tsp45I GTSAC 4 cut(s) 520, 1316, 1886, 2392
TspGWI ACGGA 4 cut(s) 218, 391, 1335, 2603
TspRI CASTG 5 cut(s) 1557, 1956, 2767, 2781, 2884
Tth111I GACNNNGTC 1 cut(s) 65
VpaK11BI GGWCC 3 cut(s) 359, 826, 1082
VspI ATTAAT 1 cut(s) 2454
XapI RAATTY 3 cut(s) 1230, 1850, 2939
XceI RCATGY 3 cut(s) 1151, 2035, 2707
XmiI GTMKAC 3 cut(s) 275, 293, 1076
XmnI GAANNNNTTC 1 cut(s) 1341
XspI CTAG 6 cut(s) 564, 1194, 2405, 2627, 2634, 2832
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.