Rmu_sc0003392.1_g000013

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003392.1
OG0006720
Physical Location & Seq
Forward (+)
54457 .. 55701
1245 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1245 bp
atgccaaaatgcccaactcactactcaaagctcctcacctcctcaatccaccaacgcctcaagaatcacaccctcttcccaattgacctttcatctccttcactccaatctcacctctcatctctttcagttccatcagacaatgacgaaccagagcccccaatttccgacgaaatcttcaggaaaggtccgaactttgggtcttataagtcaggtgattcaactttctattcactcattgagaactatgcgagtctgggggattttgggtccttggaaaaagttctggatcaaatgaaacgcgaaaggagagtctttattgagaggagtttcattgctgtgtttagagcttttgggaaagcacatttgcccaataaagctgtagacttgtttcatagaatggtagatgagtttcagtgtaggaggactgttaagtcgtttaattcggttctcaatgttattgtacaggagggtcattatgcccatgcattggagttttatgatcatgttgttggtgatagaaacatgattattttgccgaatgtgcttagttataatttgattattaaggcaatgtgtaggtttggattggttgataaggctgtcgagaagtttagggaaatgccggtcagggattgtgctcctgatgtgtttacgtattgtacattgatggatgggttgtgcaaggtgaataggattgacgaggctgtgtttttgttggatgagatgcagattgagggctgctctccgagtcctgcaacgttcaacgcgtttattgatgcagtgtgcaagaatggtgacttgggccatgcggcgaagcttgttgataatatgtttcttaaggggtgtgttcccaatgaagtgacttgtaacacacttatacatggtttgtgtctgccggggaagttgcagaaggcaatgagtcttttggatagaatggttttgaataaatgtgtgcccaatgatgtcacctacggcacaatcatcaatggacttgttaagcaaggacgatctcttgatggagctcgagtgttgatatctatggaagaaagagggcgtcgtgcaaatgagtatatttattcagttcttgttagtggattgttcaaggagggaaaatctgaagaggcaatgaaattgtggaaggagatgatggaaaagggatgtaaacccaacaccgtggtgtatagtgctcttatagaaattacacttcatttttcaccccaagttgttgctaagctgccatag

Protein Analysis

414

Amino Acids

46.55

Weight (kDa)

7.54

Isoelectric Point (pI)

45.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_long PF17177 149 - 313 8.9e-06 Pentacotripeptide-repeat region of PRORP
PPR_1 PF12854 178 - 208 2.3e-09 PPR repeat
PPR_2 PF13041 180 - 229 3e-16 PPR repeat family
PPR_3 PF13812 205 - 264 3.9e-06 Pentatricopeptide repeat domain
PPR_1 PF12854 212 - 244 4.9e-12 PPR repeat
PPR PF01535 218 - 248 9.8e-07 PPR repeat
PPR_2 PF13041 222 - 264 1.9e-10 PPR repeat family
PPR_1 PF12854 247 - 278 1.7e-07 PPR repeat
PPR_2 PF13041 251 - 298 2.5e-13 PPR repeat family
PPR_3 PF13812 273 - 333 9.5e-09 Pentatricopeptide repeat domain
PPR_1 PF12854 281 - 314 1.2e-10 PPR repeat
PPR_2 PF13041 285 - 334 7.7e-15 PPR repeat family
PPR_2 PF13041 323 - 369 7.9e-07 PPR repeat family
PPR_3 PF13812 344 - 401 2e-08 Pentatricopeptide repeat domain
PPR_1 PF12854 354 - 383 7e-06 PPR repeat
PPR_2 PF13041 356 - 399 4.5e-12 PPR repeat family
PPR PF01535 359 - 388 8.9e-08 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 207, 555
AasI GACNNNNNNGTC 1 cut(s) 433
AccB7I CCANNNNNTGG 1 cut(s) 490
AccI GTMKAC 1 cut(s) 384
AccII CGCG 2 cut(s) 303, 770
AciI CCGC 1 cut(s) 812
AclI AACGTT 1 cut(s) 761
AclWI GGATC 1 cut(s) 297
AcuI CTGAAG 2 cut(s) 163, 1140
AcyI GRCGYC 1 cut(s) 1057
AfaI GTAC 2 cut(s) 465, 664
AfiI CCNNNNNNNGG 2 cut(s) 197, 490
AflII CTTAAG 1 cut(s) 839
AflIII ACRYGT 1 cut(s) 768
AgsI TTSAA 4 cut(s) 222, 766, 946, 1105
AjuI GAANNNNNNNTTGG 4 cut(s) 73, 105, 785, 817
AleI CACNNNNGTG 1 cut(s) 1178
AluBI AGCT 6 cut(s) 31, 350, 380, 820, 1025, 1237
AluI AGCT 6 cut(s) 31, 350, 380, 820, 1025, 1237
Alw21I GWGCWC 3 cut(s) 643, 1027, 1192
AlwI GGATC 1 cut(s) 297
Ama87I CYCGRG 1 cut(s) 1026
AoxI GGCC 1 cut(s) 805
ApeKI GCWGC 2 cut(s) 741, 1237
ArsI GACNNNNNNTTYG 4 cut(s) 185, 217, 297, 329
Asp700I GAANNNNTTC 1 cut(s) 282
AspS9I GGNCC 3 cut(s) 188, 270, 805
AsuC2I CCSGG 1 cut(s) 900
AsuHPI GGTGA 8 cut(s) 28, 104, 227, 527, 700, 809, 961, 1209
AvaI CYCGRG 1 cut(s) 1026
AvaII GGWCC 2 cut(s) 188, 270
BaeGI GKGCMC 1 cut(s) 960
BanII GRGCYC 2 cut(s) 159, 1027
Bbv12I GWGCWC 3 cut(s) 643, 1027, 1192
BbvI GCAGC 2 cut(s) 728, 1224
BccI CCATC 5 cut(s) 142, 664, 668, 1013, 1144
BceAI ACGGC 1 cut(s) 991
BclI TGATCA 1 cut(s) 502
BcnI CCSGG 1 cut(s) 900
BfmI CTRYAG 1 cut(s) 381
BfrI CTTAAG 1 cut(s) 839
BisI GCNGC 3 cut(s) 742, 813, 1238
BlpI GCTNAGC 1 cut(s) 1233
BlsI GCNGC 3 cut(s) 743, 814, 1239
Bme1390I CCNGG 1 cut(s) 900
Bme18I GGWCC 2 cut(s) 188, 270
BmeT110I CYCGRG 1 cut(s) 1026
BmgT120I GGNCC 3 cut(s) 188, 270, 805
BmiI GGNNCC 1 cut(s) 271
BmrFI CCNGG 1 cut(s) 900
BmsI GCATC 2 cut(s) 717, 769
BplI GAGNNNNNCTC 2 cut(s) 728, 760
Bpu1102I GCTNAGC 1 cut(s) 1233
BpuEI CTTGAG 1 cut(s) 44
BpuMI CCSGG 1 cut(s) 900
BsaAI YACGTR 1 cut(s) 657
BsaHI GRCGYC 1 cut(s) 1057
BsaJI CCNNGG 3 cut(s) 273, 899, 1176
BsaXI ACNNNNNCTCC 2 cut(s) 1014, 1044
Bsc4I CCNNNNNNNGG 2 cut(s) 197, 490
Bse118I RCCGGY 1 cut(s) 625
Bse3DI GCAATG 4 cut(s) 333, 579, 924, 1134
BseDI CCNNGG 3 cut(s) 273, 899, 1176
BseGI GGATG 3 cut(s) 679, 727, 1166
BseLI CCNNNNNNNGG 2 cut(s) 197, 490
BseMI GCAATG 4 cut(s) 333, 579, 924, 1134
BseRI GAGGAG 3 cut(s) 23, 31, 340
BseSI GKGCMC 1 cut(s) 960
BseXI GCAGC 2 cut(s) 728, 1224
Bsh1236I CGCG 2 cut(s) 303, 770
BshFI GGCC 1 cut(s) 807
BsiHKAI GWGCWC 3 cut(s) 643, 1027, 1192
BsiHKCI CYCGRG 1 cut(s) 1026
BsiSI CCGG 2 cut(s) 626, 899
BslI CCNNNNNNNGG 2 cut(s) 197, 490
BsnI GGCC 1 cut(s) 807
BsoBI CYCGRG 1 cut(s) 1026
Bsp1286I GDGCHC 5 cut(s) 159, 643, 960, 1027, 1192
Bsp1407I TGTACA 2 cut(s) 463, 662
Bsp143I GATC 3 cut(s) 289, 502, 1010
Bsp1720I GCTNAGC 1 cut(s) 1233
BspACI CCGC 1 cut(s) 812
BspANI GGCC 1 cut(s) 807
BspFNI CGCG 2 cut(s) 303, 770
BspLI GGNNCC 1 cut(s) 271
BspPI GGATC 1 cut(s) 297
BspTI CTTAAG 1 cut(s) 839
BsrDI GCAATG 4 cut(s) 333, 579, 924, 1134
BsrFI RCCGGY 1 cut(s) 625
BsrGI TGTACA 2 cut(s) 463, 662
BssAI RCCGGY 1 cut(s) 625
BssECI CCNNGG 3 cut(s) 273, 899, 1176
BssMI GATC 3 cut(s) 289, 502, 1010
BssNI GRCGYC 1 cut(s) 1057
BssT1I CCWWGG 1 cut(s) 273
Bst4CI ACNGT 2 cut(s) 430, 1177
Bst6I CTCTTC 2 cut(s) 80, 1116
BstACI GRCGYC 1 cut(s) 1057
BstAFI CTTAAG 1 cut(s) 839
BstAUI TGTACA 2 cut(s) 463, 662
BstBAI YACGTR 1 cut(s) 657
BstDEI CTNAG 2 cut(s) 548, 1233
BstDSI CCRYGG 1 cut(s) 1176
BstF5I GGATG 3 cut(s) 679, 727, 1166
BstFNI CGCG 2 cut(s) 303, 770
BstKTI GATC 3 cut(s) 292, 505, 1013
BstMBI GATC 3 cut(s) 289, 502, 1010
BstMWI GCNNNNNNNGC 1 cut(s) 544
BstSCI CCNGG 1 cut(s) 898
BstSFI CTRYAG 1 cut(s) 381
BstSLI GKGCMC 1 cut(s) 960
BstSNI TACGTA 1 cut(s) 657
BstUI CGCG 2 cut(s) 303, 770
BstV1I GCAGC 2 cut(s) 728, 1224
BstXI CCANNNNNNTGG 1 cut(s) 1177
BsuRI GGCC 1 cut(s) 807
BtgI CCRYGG 1 cut(s) 1176
BtsCI GGATG 3 cut(s) 679, 727, 1166
BtsI GCAGTG 1 cut(s) 789
BtsIMutI CAGTG 2 cut(s) 422, 789
Cfr10I RCCGGY 1 cut(s) 625
Cfr13I GGNCC 3 cut(s) 188, 270, 805
CseI GACGC 1 cut(s) 1046
Csp6I GTAC 2 cut(s) 464, 663
CviAII CATG 5 cut(s) 485, 506, 526, 809, 884
CviQI GTAC 2 cut(s) 464, 663
DdeI CTNAG 2 cut(s) 548, 1233
DpnI GATC 3 cut(s) 291, 504, 1012
DpnII GATC 3 cut(s) 289, 502, 1010
DrdI GACNNNNNNGTC 1 cut(s) 433
DseDI GACNNNNNNGTC 1 cut(s) 433
Eam1104I CTCTTC 2 cut(s) 80, 1116
EarI CTCTTC 2 cut(s) 80, 1116
Ecl136II GAGCTC 1 cut(s) 1025
Eco105I TACGTA 1 cut(s) 657
Eco130I CCWWGG 1 cut(s) 273
Eco24I GRGCYC 2 cut(s) 159, 1027
Eco32I GATATC 1 cut(s) 1038
Eco47I GGWCC 2 cut(s) 188, 270
Eco53kI GAGCTC 1 cut(s) 1025
Eco57I CTGAAG 2 cut(s) 163, 1140
Eco88I CYCGRG 1 cut(s) 1026
EcoICRI GAGCTC 1 cut(s) 1025
EcoO109I RGGNCCY 1 cut(s) 270
EcoRV GATATC 1 cut(s) 1038
EcoT14I CCWWGG 1 cut(s) 273
EcoT22I ATGCAT 1 cut(s) 490
EcoT38I GRGCYC 2 cut(s) 159, 1027
ErhI CCWWGG 1 cut(s) 273
FaeI CATG 5 cut(s) 488, 509, 529, 812, 887
FatI CATG 5 cut(s) 484, 505, 525, 808, 883
FbaI TGATCA 1 cut(s) 502
FblI GTMKAC 1 cut(s) 384
Fnu4HI GCNGC 3 cut(s) 742, 813, 1238
FokI GGATG 3 cut(s) 686, 734, 1173
FriOI GRGCYC 2 cut(s) 159, 1027
Fsp4HI GCNGC 3 cut(s) 742, 813, 1238
GluI GCNGC 3 cut(s) 742, 813, 1238
HaeIII GGCC 1 cut(s) 807
HapII CCGG 2 cut(s) 626, 899
HgaI GACGC 1 cut(s) 1046
Hin1I GRCGYC 1 cut(s) 1057
Hin1II CATG 5 cut(s) 488, 509, 529, 812, 887
HindIII AAGCTT 1 cut(s) 818
HinfI GANTC 6 cut(s) 64, 218, 253, 312, 751, 922
HpaII CCGG 2 cut(s) 626, 899
HphI GGTGA 8 cut(s) 28, 104, 227, 527, 700, 809, 961, 1209
Hpy166II GTNNAC 3 cut(s) 385, 654, 1166
Hpy188I TCNGA 5 cut(s) 139, 169, 192, 750, 1120
Hpy188III TCNNGA 6 cut(s) 61, 181, 287, 607, 644, 1016
Hpy8I GTNNAC 3 cut(s) 385, 654, 1166
Hpy99I CGWCG 2 cut(s) 173, 1062
HpyAV CCTTC 3 cut(s) 108, 907, 1135
HpyCH4III ACNGT 2 cut(s) 430, 1177
HpyCH4IV ACGT 2 cut(s) 656, 761
HpyCH4V TGCA 8 cut(s) 488, 684, 730, 758, 782, 789, 910, 1064
HpyF10VI GCNNNNNNNGC 1 cut(s) 544
HpyF3I CTNAG 2 cut(s) 548, 1233
HpySE526I ACGT 2 cut(s) 656, 761
Hsp92I GRCGYC 1 cut(s) 1057
Hsp92II CATG 5 cut(s) 488, 509, 529, 812, 887
Ksp22I TGATCA 1 cut(s) 502
Kzo9I GATC 3 cut(s) 289, 502, 1010
LmnI GCTCC 3 cut(s) 36, 646, 1022
Lsp1109I GCAGC 2 cut(s) 728, 1224
LweI GCATC 2 cut(s) 717, 769
MaeII ACGT 2 cut(s) 656, 761
MaeIII GTNAC 4 cut(s) 797, 862, 869, 967
MalI GATC 3 cut(s) 291, 504, 1012
MboI GATC 3 cut(s) 289, 502, 1010
MboII GAAGA 4 cut(s) 67, 169, 1058, 1133
MfeI CAATTG 1 cut(s) 81
MhlI GDGCHC 5 cut(s) 159, 643, 960, 1027, 1192
MluCI AATT 6 cut(s) 81, 162, 442, 556, 1133, 1200
MluI ACGCGT 1 cut(s) 768
MlyI GAGTC 4 cut(s) 262, 321, 760, 931
MmeI TCCRAC 2 cut(s) 192, 699
Mph1103I ATGCAT 1 cut(s) 490
MroXI GAANNNNTTC 1 cut(s) 282
MseI TTAA 5 cut(s) 432, 441, 567, 840, 999
MslI CAYNNNNRTG 2 cut(s) 338, 1178
MspCI CTTAAG 1 cut(s) 839
MspI CCGG 2 cut(s) 626, 899
MspR9I CCNGG 1 cut(s) 900
MunI CAATTG 1 cut(s) 81
MvnI CGCG 2 cut(s) 303, 770
MwoI GCNNNNNNNGC 1 cut(s) 544
NciI CCSGG 1 cut(s) 900
NdeII GATC 3 cut(s) 289, 502, 1010
NlaIII CATG 5 cut(s) 488, 509, 529, 812, 887
NlaIV GGNNCC 1 cut(s) 271
NmuCI GTSAC 3 cut(s) 797, 862, 967
NsiI ATGCAT 1 cut(s) 490
OliI CACNNNNGTG 1 cut(s) 1178
PaeR7I CTCGAG 1 cut(s) 1026
PcsI WCGNNNNNNNCGW 1 cut(s) 767
PdmI GAANNNNTTC 1 cut(s) 282
PfeI GAWTC 2 cut(s) 64, 218
PflMI CCANNNNNTGG 1 cut(s) 490
PkrI GCNGC 3 cut(s) 743, 814, 1239
PleI GAGTC 4 cut(s) 261, 320, 759, 930
PpsI GAGTC 4 cut(s) 261, 320, 759, 930
Ppu21I YACGTR 1 cut(s) 657
PpuMI RGGWCCY 1 cut(s) 270
PsiI TTATAA 2 cut(s) 207, 555
Psp124BI GAGCTC 1 cut(s) 1027
Psp1406I AACGTT 1 cut(s) 761
Psp5II RGGWCCY 1 cut(s) 270
PspN4I GGNNCC 1 cut(s) 271
PspPI GGNCC 3 cut(s) 188, 270, 805
PspPPI RGGWCCY 1 cut(s) 270
PspXI VCTCGAGB 1 cut(s) 1026
RsaI GTAC 2 cut(s) 465, 664
RsaNI GTAC 2 cut(s) 464, 663
RseI CAYNNNNRTG 2 cut(s) 338, 1178
SacI GAGCTC 1 cut(s) 1027
SaqAI TTAA 5 cut(s) 432, 441, 567, 840, 999
SatI GCNGC 3 cut(s) 742, 813, 1238
Sau3AI GATC 3 cut(s) 289, 502, 1010
Sau96I GGNCC 3 cut(s) 188, 270, 805
SchI GAGTC 4 cut(s) 262, 321, 760, 931
ScrFI CCNGG 1 cut(s) 900
SduI GDGCHC 5 cut(s) 159, 643, 960, 1027, 1192
SfaNI GCATC 2 cut(s) 717, 769
SfcI CTRYAG 1 cut(s) 381
Sfr274I CTCGAG 1 cut(s) 1026
SinI GGWCC 2 cut(s) 188, 270
SlaI CTCGAG 1 cut(s) 1026
SmiMI CAYNNNNRTG 2 cut(s) 338, 1178
SmlI CTYRAG 3 cut(s) 59, 839, 1026
SmoI CTYRAG 3 cut(s) 59, 839, 1026
SnaBI TACGTA 1 cut(s) 657
Sse9I AATT 6 cut(s) 81, 162, 442, 556, 1133, 1200
SsiI CCGC 1 cut(s) 812
SstI GAGCTC 1 cut(s) 1027
StyD4I CCNGG 1 cut(s) 898
StyI CCWWGG 1 cut(s) 273
TaaI ACNGT 2 cut(s) 430, 1177
TaiI ACGT 2 cut(s) 659, 764
TaqI TCGA 2 cut(s) 606, 1027
TasI AATT 6 cut(s) 81, 162, 442, 556, 1133, 1200
TatI WGTACW 2 cut(s) 463, 662
TauI GCSGC 1 cut(s) 815
TfiI GAWTC 2 cut(s) 64, 218
Tru1I TTAA 5 cut(s) 432, 441, 567, 840, 999
Tru9I TTAA 5 cut(s) 432, 441, 567, 840, 999
TscAI CASTG 2 cut(s) 422, 789
TseFI GTSAC 3 cut(s) 797, 862, 967
TseI GCWGC 2 cut(s) 741, 1237
Tsp45I GTSAC 3 cut(s) 797, 862, 967
TspDTI ATGAA 7 cut(s) 81, 311, 322, 383, 873, 1145, 1199
TspRI CASTG 2 cut(s) 422, 789
Van91I CCANNNNNTGG 1 cut(s) 490
Vha464I CTTAAG 1 cut(s) 839
VpaK11BI GGWCC 2 cut(s) 188, 270
XhoI CTCGAG 1 cut(s) 1026
XmiI GTMKAC 1 cut(s) 384
XmnI GAANNNNTTC 1 cut(s) 282
Zsp2I ATGCAT 1 cut(s) 490
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.