Rmu_sc0003454.1_g000024

1-acyl-sn-glycerol-3-phosphate acyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003454.1
OG0013710
Physical Location & Seq
Reverse (-)
103554 .. 104138
585 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 585 bp
atgagaggtgaggcctttgtctctgcagtgaaacacatgaggtcttttgttccagccgtgtatgatgttacaatcgccttccctgaagctcgatcagattcacctcaaacactgttacaactcttctacgagaggctgaggcgacgaaagtcatatgtggtgcatgtgcatgttaagcggcatgagattaggggtttgcctcgtaaacatggtgcccttgagaaatggtgtgagggtgtgctggaggccaagcaagccttattggaccaacacagagcagaccaaacttttggtgaccaagtacaagtactagaaacatgctcaggtaaacgccggccactcaagcctcttttggttgttacctcttgggcacttgtatttgtattctcggcaatcaagttccccctcttatctttactagtgctactcattgcatcctcaggcctgacttactttctcaatcggggccgcttgtcttccctactgctgaatcttactatccttcttagacgtgcttttggttctcaagttgcatcacttctcctcattagtgcttcttatttcatttacttccttccttcttag

Protein Analysis

194

Amino Acids

22.04

Weight (kDa)

10.27

Isoelectric Point (pI)

47.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Acyltransf_C PF16076 47 - 123 1.9e-12 Acyltransferase C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 212
AciI CCGC 2 cut(s) 178, 469
AcoI YGGCCR 1 cut(s) 335
AcuI CTGAAG 1 cut(s) 105
AfaI GTAC 2 cut(s) 303, 309
AhlI ACTAGT 1 cut(s) 418
AjiI CACGTC 1 cut(s) 512
AluBI AGCT 1 cut(s) 89
AluI AGCT 1 cut(s) 89
Alw26I GTCTC 1 cut(s) 25
AoxI GGCC 5 cut(s) 12, 246, 335, 442, 466
ArsI GACNNNNNNTTYG 2 cut(s) 272, 304
AspS9I GGNCC 2 cut(s) 265, 466
AsuHPI GGTGA 3 cut(s) 20, 93, 305
AvaII GGWCC 1 cut(s) 265
AxyI CCTNAGG 1 cut(s) 439
BaeGI GKGCMC 2 cut(s) 217, 373
BanI GGYRCC 1 cut(s) 212
BbsI GAAGAC 1 cut(s) 468
BbvCI CCTCAGC 1 cut(s) 137
BceAI ACGGC 1 cut(s) 41
BcoDI GTCTC 1 cut(s) 25
BcuI ACTAGT 1 cut(s) 418
BfaI CTAG 2 cut(s) 311, 419
BfmI CTRYAG 1 cut(s) 24
BisI GCNGC 2 cut(s) 179, 469
BlsI GCNGC 2 cut(s) 180, 470
BmcAI AGTACT 1 cut(s) 309
Bme18I GGWCC 1 cut(s) 265
BmgBI CACGTC 1 cut(s) 512
BmgT120I GGNCC 2 cut(s) 265, 466
BmiI GGNNCC 2 cut(s) 214, 467
BmsI GCATC 2 cut(s) 443, 542
BoxI GACNNNNGTC 1 cut(s) 148
BpiI GAAGAC 1 cut(s) 468
BpmI CTGGAG 1 cut(s) 263
Bpu10I CCTNAGC 2 cut(s) 137, 322
BpuEI CTTGAG 3 cut(s) 239, 326, 510
Bse118I RCCGGY 1 cut(s) 333
Bse21I CCTNAGG 1 cut(s) 439
Bse3DI GCAATG 1 cut(s) 429
BseGI GGATG 1 cut(s) 434
BseMI GCAATG 1 cut(s) 429
BseMII CTCAG 3 cut(s) 128, 336, 453
BseRI GAGGAG 1 cut(s) 533
BseSI GKGCMC 2 cut(s) 217, 373
BshFI GGCC 5 cut(s) 14, 248, 337, 444, 468
BshNI GGYRCC 1 cut(s) 212
BsiSI CCGG 1 cut(s) 334
BsmAI GTCTC 1 cut(s) 25
BsnI GGCC 5 cut(s) 14, 248, 337, 444, 468
Bsp1286I GDGCHC 2 cut(s) 217, 373
Bsp143I GATC 1 cut(s) 92
BspACI CCGC 2 cut(s) 178, 469
BspANI GGCC 5 cut(s) 14, 248, 337, 444, 468
BspCNI CTCAG 3 cut(s) 129, 335, 452
BspLI GGNNCC 2 cut(s) 214, 467
BspMAI CTGCAG 1 cut(s) 28
BspT107I GGYRCC 1 cut(s) 212
BsrDI GCAATG 1 cut(s) 429
BsrFI RCCGGY 1 cut(s) 333
BssAI RCCGGY 1 cut(s) 333
BssMI GATC 1 cut(s) 92
Bst4CI ACNGT 1 cut(s) 114
Bst6I CTCTTC 1 cut(s) 128
BstC8I GCNNGC 2 cut(s) 255, 335
BstDEI CTNAG 5 cut(s) 137, 322, 439, 506, 582
BstEII GGTNACC 1 cut(s) 293
BstF5I GGATG 1 cut(s) 434
BstKTI GATC 1 cut(s) 95
BstMAI GTCTC 1 cut(s) 25
BstMBI GATC 1 cut(s) 92
BstMWI GCNNNNNNNGC 3 cut(s) 175, 254, 343
BstNSI RCATGY 3 cut(s) 167, 173, 321
BstPAI GACNNNNGTC 1 cut(s) 148
BstPI GGTNACC 1 cut(s) 293
BstSFI CTRYAG 1 cut(s) 24
BstSLI GKGCMC 2 cut(s) 217, 373
BstV2I GAAGAC 1 cut(s) 468
BstXI CCANNNNNNTGG 1 cut(s) 290
Bsu36I CCTNAGG 1 cut(s) 439
BsuRI GGCC 5 cut(s) 14, 248, 337, 444, 468
BtrI CACGTC 1 cut(s) 512
BtsCI GGATG 1 cut(s) 434
BtsI GCAGTG 1 cut(s) 33
BtsIMutI CAGTG 2 cut(s) 33, 110
Cac8I GCNNGC 2 cut(s) 255, 335
Cfr10I RCCGGY 1 cut(s) 333
Cfr13I GGNCC 2 cut(s) 265, 466
Csp6I GTAC 2 cut(s) 302, 308
CviAII CATG 6 cut(s) 37, 164, 170, 182, 209, 318
CviQI GTAC 2 cut(s) 302, 308
DdeI CTNAG 5 cut(s) 137, 322, 439, 506, 582
DpnI GATC 1 cut(s) 94
DpnII GATC 1 cut(s) 92
EaeI YGGCCR 1 cut(s) 335
Eam1104I CTCTTC 1 cut(s) 128
EarI CTCTTC 1 cut(s) 128
Eco147I AGGCCT 2 cut(s) 14, 444
Eco47I GGWCC 1 cut(s) 265
Eco57I CTGAAG 1 cut(s) 105
Eco81I CCTNAGG 1 cut(s) 439
Eco91I GGTNACC 1 cut(s) 293
EcoO65I GGTNACC 1 cut(s) 293
FaeI CATG 6 cut(s) 40, 167, 173, 185, 212, 321
FaiI YATR 9 cut(s) 38, 63, 154, 156, 165, 171, 183, 210, 319
FalI AAGNNNNNCTT 2 cut(s) 242, 274
FatI CATG 6 cut(s) 36, 163, 169, 181, 208, 317
FauNDI CATATG 1 cut(s) 154
Fnu4HI GCNGC 2 cut(s) 179, 469
FokI GGATG 1 cut(s) 421
Fsp4HI GCNGC 2 cut(s) 179, 469
FspBI CTAG 2 cut(s) 311, 419
GluI GCNGC 2 cut(s) 179, 469
GsuI CTGGAG 1 cut(s) 263
HaeIII GGCC 5 cut(s) 14, 248, 337, 444, 468
HapII CCGG 1 cut(s) 334
Hin1II CATG 6 cut(s) 40, 167, 173, 185, 212, 321
HinfI GANTC 2 cut(s) 98, 490
HpaII CCGG 1 cut(s) 334
HphI GGTGA 3 cut(s) 20, 93, 305
Hpy166II GTNNAC 2 cut(s) 206, 329
Hpy188I TCNGA 1 cut(s) 97
Hpy8I GTNNAC 2 cut(s) 206, 329
Hpy99I CGWCG 1 cut(s) 147
HpyAV CCTTC 3 cut(s) 88, 512, 584
HpyCH4III ACNGT 1 cut(s) 114
HpyCH4IV ACGT 1 cut(s) 511
HpyCH4V TGCA 5 cut(s) 26, 163, 169, 434, 533
HpyF10VI GCNNNNNNNGC 3 cut(s) 175, 254, 343
HpyF3I CTNAG 5 cut(s) 137, 322, 439, 506, 582
HpySE526I ACGT 1 cut(s) 511
Hsp92II CATG 6 cut(s) 40, 167, 173, 185, 212, 321
KroI GCCGGC 1 cut(s) 333
KroNI GCCGGC 1 cut(s) 335
Kzo9I GATC 1 cut(s) 92
LpnPI CCDG 7 cut(s) 66, 96, 227, 309, 347, 426, 458
LweI GCATC 2 cut(s) 443, 542
MaeI CTAG 2 cut(s) 311, 419
MaeII ACGT 1 cut(s) 511
MaeIII GTNAC 4 cut(s) 67, 114, 293, 358
MalI GATC 1 cut(s) 94
MboI GATC 1 cut(s) 92
MboII GAAGA 2 cut(s) 115, 468
MhlI GDGCHC 2 cut(s) 217, 373
MroNI GCCGGC 1 cut(s) 333
MseI TTAA 1 cut(s) 174
MslI CAYNNNNRTG 1 cut(s) 168
MspI CCGG 1 cut(s) 334
MwoI GCNNNNNNNGC 3 cut(s) 175, 254, 343
NaeI GCCGGC 1 cut(s) 335
NdeI CATATG 1 cut(s) 154
NdeII GATC 1 cut(s) 92
NgoMIV GCCGGC 1 cut(s) 333
NlaIII CATG 6 cut(s) 40, 167, 173, 185, 212, 321
NlaIV GGNNCC 2 cut(s) 214, 467
NmeAIII GCCGAG 1 cut(s) 368
NmuCI GTSAC 1 cut(s) 293
NspI RCATGY 3 cut(s) 167, 173, 321
PceI AGGCCT 2 cut(s) 14, 444
PdiI GCCGGC 1 cut(s) 335
PfeI GAWTC 2 cut(s) 98, 490
PkrI GCNGC 2 cut(s) 180, 470
PshAI GACNNNNGTC 1 cut(s) 148
PspEI GGTNACC 1 cut(s) 293
PspN4I GGNNCC 2 cut(s) 214, 467
PspPI GGNCC 2 cut(s) 265, 466
PstI CTGCAG 1 cut(s) 28
RsaI GTAC 2 cut(s) 303, 309
RsaNI GTAC 2 cut(s) 302, 308
RseI CAYNNNNRTG 1 cut(s) 168
SaqAI TTAA 1 cut(s) 174
SatI GCNGC 2 cut(s) 179, 469
Sau3AI GATC 1 cut(s) 92
Sau96I GGNCC 2 cut(s) 265, 466
ScaI AGTACT 1 cut(s) 309
SduI GDGCHC 2 cut(s) 217, 373
SetI ASST 7 cut(s) 10, 44, 91, 106, 328, 365, 514
SfaNI GCATC 2 cut(s) 443, 542
SfcI CTRYAG 1 cut(s) 24
SinI GGWCC 1 cut(s) 265
SmiMI CAYNNNNRTG 1 cut(s) 168
SmlI CTYRAG 3 cut(s) 218, 341, 525
SmoI CTYRAG 3 cut(s) 218, 341, 525
SpeI ACTAGT 1 cut(s) 418
SseBI AGGCCT 2 cut(s) 14, 444
SsiI CCGC 2 cut(s) 178, 469
SspMI CTAG 2 cut(s) 311, 419
StuI AGGCCT 2 cut(s) 14, 444
TaaI ACNGT 1 cut(s) 114
TaiI ACGT 1 cut(s) 514
TaqI TCGA 1 cut(s) 91
TatI WGTACW 2 cut(s) 301, 307
TauI GCSGC 2 cut(s) 181, 471
TfiI GAWTC 2 cut(s) 98, 490
Tru1I TTAA 1 cut(s) 174
Tru9I TTAA 1 cut(s) 174
TscAI CASTG 2 cut(s) 33, 117
TseFI GTSAC 1 cut(s) 293
Tsp45I GTSAC 1 cut(s) 293
TspDTI ATGAA 1 cut(s) 553
TspRI CASTG 2 cut(s) 33, 117
VpaK11BI GGWCC 1 cut(s) 265
XceI RCATGY 3 cut(s) 167, 173, 321
XspI CTAG 2 cut(s) 311, 419
ZrmI AGTACT 1 cut(s) 309
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.