Rmu_sc0003503.1_g000001

This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003503.1
OG0015798
Physical Location & Seq
Forward (+)
1 .. 4386
4386 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 3033 bp
acttctattcccttctcagccatctacttttctttgtgtaacacagctggtaataaagaagctggatacagtctttccaaagaagttagtgatgctggttttggcatttcacctgatgagcttgcatcctttgcgcgaacccatgataccaagggtgtggaagcacgaggaggagtagcaggactagcaggagatatatcagtatccttgaaagaaggcgttgtttcaagcaagatacctgttaggcaaaacatatatggcctcaaccgatttgtggagaagccttctaaaggtttttggatgtttgtctgggaagccatacaagatttaactctaatcatccttatggtttctgctgtggtctctattggcgttggcattgcaactgaagggtggccaaagggtatgtatgatggattgggtataatattgagcatcttattggttgtgatggtcactgcaatcagtgactacaggcaatctttgcaatttaaagaactggataaggaaaaaaaaaatataaagattcaggtaactagagatggacgcagacagaaggtttctatctacgatttggtggttggagatattgttcatctttcaattggagaccaagttccagctgatggccttctcatatcaggccacagcttacaaattgatgaatcaagcttgtcaggtgagagcgagccagtggaaataaatcaaaagagaccttttattcttggaggaaccaaagtgcaagatggctctggtaaaatgctagttacatctgttggtatgaggactgaatggggacgactaatggttactctgagtgaaggaggagacaatgagacaccactgcaggtgaaactcaatggtgttgctaccattattggaaaaattggtttggcttttgcagctgtcacatttgtagttctgactacgaggtacttagttaacaagggaacgcaccataacatgactaactggtctatgaaagatgcatcagcacttttgagttacttttctactgcagttatcataatagttgttgctgttccagaggggcttcctctggcagtgacactgagtcttgcctttgcaatgaaaaagctaatgggtgacaaggcacttgtgaggcatctttcagcatgtgagacgatgggctctgctagttgtatttgcacagataagacgggaaccttgacaacaaatcatatggtggtgaataagatatggatatgtgacgaagccaagactataacgagtggtcagaacaaagatgtattgaagtcagtttctgaaaaagtttataacattcttttgcagtctatctttctgaatacaacctcagaggtagtaaaaggggaagatgggaaaaatactgttataggtactccaacagagatggctatagtagaatttggcatgcttctgggaggtgattttaaatcttttagtgaggagtataagatagttatggtcgaaccattcaattcaatcagaaaaaagatgtcggttcaagtggctcttcctggtagtgatatgtatcgagcattctgcaaaggagcatcagaaattattttaggaatgtgtgacaaggttgtcaatactgatggtgaagctatgcctttgtctgaggagcagagaaagaaaatatctgatgtcataaatggttttgcttgtgaagctttaagaactctatgcgtagctttcaaggatattgcggacccttctagtgcaaaaagtattcctgaagataaatatacattaatagctgttgtgggaattaaggatcctgtgcgccctggcgtgagggaagccgtgaagacttgtttagatgctgggattactgtacggatggtcactggtgataatatcaatacagctaaagccatagctaaagagtgtggcattttgacagaagatggtttagctatagaagggccagatttccgaaaaatgagccaacaggagatggcagaaattatcccaaaactgcaggtaatggcacgatccttgcctttggacaagcacactttagtgaagcagttgaggaatgtgcatcaagaagtagtggcagtaactggtgatgggactaatgatgctcctgctttgcacgaggcagacattggactcgctatggtgaagcagttgaggaatgtgcataaagaagtagtggcagtaactggtgatgggactaatgatgctcctgctttgcacgaggcagacattggactcgctatgggtatagccggaacagaggttgcaaaagaaaatgctgatgttatcattatggatgacaactttacaagtatagtgaatgttgcaagatggggtcgttcagtttatatcaacattcaaaagtttgttcagttccagttaacggttaatattgtcgctctgatgctcaatttcgtatccgcatgcgtctcaggatcagctcctctcactgctgtccagatgctttgggtaaacttgattatggatactcttggtgctttggcactggctacagagccaccgcatgatggccttatgaaaagaccaccagttggaaggaatattaaattcatcactgggatcatgtggaggaatattatcggtcagagtatctatcaaattgctgtccttctggttcttaatttttgtgggaaacgtcttttaaaactcaccggtccaaatgcaagttccattctcaagaccttcatattcaactgttttgtattttgccaggtattcaacgagattaacagccgtgatatggagaaaatcaatgtgttcagagggatgtttaatagttatactttcatgatagtgatgatctgcacagtagttttccagatcataatagttgaatttttgggcacttttgctcaaactgtgccactgagctggaagttgtggttagcaagcgtcttgattggagccgtaagtttaccagttgctgttgtcttgaagtgcattcctgtatcattaaggaaacaagctgctaaggatcacagtgatatccacgaacctcttcttcgtggccccgagctggcttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000325 GO:0001101 GO:0003674 GO:0003824 GO:0005215 GO:0005388 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005794 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0006950 GO:0006952 GO:0008150 GO:0008324 GO:0009266 GO:0009409 GO:0009414 GO:0009415 GO:0009507 GO:0009536 GO:0009605 GO:0009607 GO:0009617 GO:0009628 GO:0009705 GO:0010035 GO:0010941 GO:0012505 GO:0015075 GO:0015085 GO:0015318 GO:0015399 GO:0015405 GO:0015662 GO:0016020 GO:0016021 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019829 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030001 GO:0031090 GO:0031224 GO:0031226 GO:0034220 GO:0042221 GO:0042592 GO:0042623 GO:0042625 GO:0042626 GO:0042742 GO:0043067 GO:0043069 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044422 GO:0044424 GO:0044425 GO:0044437 GO:0044444 GO:0044446 GO:0044459 GO:0044464 GO:0046873 GO:0048519 GO:0048523 GO:0048878 GO:0050789 GO:0050794 GO:0050801 GO:0050896 GO:0051179 GO:0051234 GO:0051704 GO:0051707 GO:0055081 GO:0055085 GO:0060548 GO:0065007 GO:0065008 GO:0070588 GO:0070838 GO:0071944 GO:0072511 GO:0090662 GO:0098542 GO:0098588 GO:0098655 GO:0098660 GO:0098662 GO:0098805 GO:0099131 GO:0099132 GO:1901700
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CaATP_NAI Cation_ATPase Cation_ATPase_C Cation_ATPase_N E1-E2_ATPase Hydrolase Hydrolase_3

Protein Analysis

1010

Amino Acids

109.57

Weight (kDa)

6.59

Isoelectric Point (pI)

34.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cation_ATPase_N PF00690 56 - 122 2.2e-10 Cation transporter/ATPase, N-terminus
E1-E2_ATPase PF00122 175 - 269 5.7e-23 P-type ATPase actuator domain
Cation_ATPase PF13246 435 - 598 3.4e-33 P-type ATPase, cytoplasmic domain N
Hydrolase PF00702 583 - 705 2.3e-12 haloacid dehalogenase-like hydrolase
Cation_ATPase_C PF00689 810 - 983 4.1e-41 Cation transporting ATPase, C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015798)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g01980 FvH4_7g01980
malus_domestica MD02G1298100.v1.1 MD07G1026000.v1.1
prunus_persica Prupe.2G026200_v2.0.a1
pyrus_communis pycom07g01980
rosa_chinensis RchiOBHm_Chr1g0318981
rosa_laevigata RLG00000030512
rosa_multiflora Rmu_sc0003503.1_g000001 Rmu_sc0006626.1_g000004
rosa_roxburghii Rroxscaffold_4G00328710
rosa_rugosa Rorug01G0026100 Rorug01G0026200
rosa_samantha Rh1AG038000
rosa_wichuraiana Rw1G002930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1299
AarI CACCTGC 1 cut(s) 838
AasI GACNNNNNNGTC 2 cut(s) 973, 1589
Acc36I ACCTGC 2 cut(s) 838, 1978
AccB7I CCANNNNNTGG 2 cut(s) 626, 2540
AccII CGCG 1 cut(s) 136
AciI CCGC 3 cut(s) 1712, 2409, 2510
AclWI GGATC 6 cut(s) 1775, 1788, 1995, 2431, 2576, 2991
AcoI YGGCCR 1 cut(s) 395
AcsI RAATTY 3 cut(s) 1406, 2555, 2843
AcuI CTGAAG 2 cut(s) 408, 1761
AfaI GTAC 3 cut(s) 935, 1381, 1842
AfiI CCNNNNNNNGG 8 cut(s) 274, 290, 626, 2371, 2516, 2540, 2749, 3025
AgeI ACCGGT 1 cut(s) 2660
AhdI GACNNNNNGTC 1 cut(s) 1074
AjnI CCWGG 3 cut(s) 1519, 1792, 2718
AjuI GAANNNNNNNTTGG 2 cut(s) 2659, 2691
AleI CACNNNNGTG 1 cut(s) 2027
AloI GAACNNNNNNTCC 4 cut(s) 576, 600, 608, 632
Alw26I GTCTC 7 cut(s) 367, 603, 706, 822, 830, 1136, 2422
AlwI GGATC 6 cut(s) 1775, 1788, 1995, 2431, 2576, 2991
AlwNI CAGNNNCTG 4 cut(s) 1286, 2072, 2174, 2933
Ama87I CYCGRG 1 cut(s) 3020
AoxI GGCC 7 cut(s) 259, 395, 628, 643, 1931, 2518, 3016
ApeKI GCWGC 2 cut(s) 902, 2975
ApoI RAATTY 3 cut(s) 1406, 2555, 2843
AseI ATTAAT 1 cut(s) 1757
AsiGI ACCGGT 1 cut(s) 2660
Asp700I GAANNNNTTC 1 cut(s) 3006
AspLEI GCGC 2 cut(s) 136, 1791
AspS9I GGNCC 4 cut(s) 1714, 1931, 2663, 3017
AvaI CYCGRG 1 cut(s) 3020
AvaII GGWCC 2 cut(s) 1714, 2663
BaeGI GKGCMC 1 cut(s) 2855
BalI TGGCCA 1 cut(s) 397
BamHI GGATCC 1 cut(s) 1780
BanII GRGCYC 1 cut(s) 1154
BauI CACGAG 3 cut(s) 165, 2105, 2207
BbsI GAAGAC 1 cut(s) 1820
BbvI GCAGC 2 cut(s) 914, 2962
BceAI ACGGC 3 cut(s) 1793, 2727, 2900
BcgI CGANNNNNNTGC 2 cut(s) 1527, 1561
BciT130I CCWGG 3 cut(s) 1521, 1794, 2720
BciVI GTATCC 4 cut(s) 59, 214, 2416, 2467
BcoDI GTCTC 7 cut(s) 367, 603, 706, 822, 830, 1136, 2422
BfaI CTAG 5 cut(s) 185, 537, 764, 1158, 1722
BfmI CTRYAG 7 cut(s) 472, 845, 1017, 1398, 1923, 1985, 2499
BfuAI ACCTGC 2 cut(s) 838, 1978
BfuI GTATCC 4 cut(s) 59, 214, 2416, 2467
BisI GCNGC 2 cut(s) 903, 2976
BlsI GCNGC 2 cut(s) 904, 2977
Bme1390I CCNGG 3 cut(s) 1521, 1794, 2720
Bme18I GGWCC 2 cut(s) 1714, 2663
BmeRI GACNNNNNGTC 1 cut(s) 1074
BmeT110I CYCGRG 1 cut(s) 3020
BmgT120I GGNCC 4 cut(s) 1714, 1931, 2663, 3017
BmiI GGNNCC 6 cut(s) 733, 1186, 1716, 1782, 2914, 3019
BmrFI CCNGG 3 cut(s) 1521, 1794, 2720
BmrI ACTGGG 1 cut(s) 2574
BmuI ACTGGG 1 cut(s) 2574
BpiI GAAGAC 1 cut(s) 1820
Bpu10I CCTNAGC 1 cut(s) 2979
BpuEI CTTGAG 1 cut(s) 2669
BsaBI GATNNNNATC 1 cut(s) 1533
BsaI GGTCTC 3 cut(s) 367, 603, 706
BsaJI CCNNGG 2 cut(s) 150, 1792
BsaWI WCCGGW 1 cut(s) 2660
BsaXI ACNNNNNCTCC 4 cut(s) 576, 600, 606, 630
Bsc4I CCNNNNNNNGG 8 cut(s) 274, 290, 626, 2371, 2516, 2540, 2749, 3025
Bse118I RCCGGY 1 cut(s) 2660
Bse3DI GCAATG 2 cut(s) 378, 1095
Bse8I GATNNNNATC 1 cut(s) 1533
BseBI CCWGG 3 cut(s) 1521, 1794, 2720
BseDI CCNNGG 2 cut(s) 150, 1792
BseGI GGATG 6 cut(s) 125, 306, 339, 1851, 2290, 2781
BseJI GATNNNNATC 1 cut(s) 1533
BseLI CCNNNNNNNGG 8 cut(s) 274, 290, 626, 2371, 2516, 2540, 2749, 3025
BseMI GCAATG 2 cut(s) 378, 1095
BseMII CTCAG 7 cut(s) 30, 806, 1064, 1350, 1614, 2433, 2867
BseRI GAGGAG 6 cut(s) 183, 186, 840, 1463, 1640, 2421
BseSI GKGCMC 1 cut(s) 2855
BseXI GCAGC 2 cut(s) 914, 2962
BseYI CCCAGC 1 cut(s) 1829
BsgI GTGCAG 1 cut(s) 2797
Bsh1236I CGCG 1 cut(s) 136
BshFI GGCC 7 cut(s) 261, 397, 630, 645, 1933, 2520, 3018
BshTI ACCGGT 1 cut(s) 2660
BsiHKCI CYCGRG 1 cut(s) 3020
BsiSI CCGG 2 cut(s) 2241, 2661
BslFI GGGAC 3 cut(s) 810, 2095, 2197
BslI CCNNNNNNNGG 8 cut(s) 274, 290, 626, 2371, 2516, 2540, 2749, 3025
BsmAI GTCTC 7 cut(s) 367, 603, 706, 822, 830, 1136, 2422
BsmBI CGTCTC 2 cut(s) 1136, 2422
BsmFI GGGAC 3 cut(s) 810, 2095, 2197
BsmI GAATGC 2 cut(s) 1541, 2949
BsnI GGCC 7 cut(s) 261, 397, 630, 645, 1933, 2520, 3018
Bso31I GGTCTC 3 cut(s) 367, 603, 706
BsoBI CYCGRG 1 cut(s) 3020
Bsp1286I GDGCHC 2 cut(s) 1154, 2855
Bsp143I GATC 7 cut(s) 1780, 2000, 2423, 2568, 2808, 2829, 2983
BspACI CCGC 3 cut(s) 1712, 2409, 2510
BspANI GGCC 7 cut(s) 261, 397, 630, 645, 1933, 2520, 3018
BspCNI CTCAG 7 cut(s) 29, 807, 1065, 1349, 1615, 2432, 2868
BspFNI CGCG 1 cut(s) 136
BspHI TCATGA 1 cut(s) 2796
BspLI GGNNCC 6 cut(s) 733, 1186, 1716, 1782, 2914, 3019
BspMAI CTGCAG 3 cut(s) 849, 1021, 1989
BspMI ACCTGC 2 cut(s) 838, 1978
BspPI GGATC 6 cut(s) 1775, 1788, 1995, 2431, 2576, 2991
BspQI GCTCTTC 1 cut(s) 1521
BspTNI GGTCTC 3 cut(s) 367, 603, 706
BsrDI GCAATG 2 cut(s) 378, 1095
BsrFI RCCGGY 1 cut(s) 2660
BssAI RCCGGY 1 cut(s) 2660
BssECI CCNNGG 2 cut(s) 150, 1792
BssMI GATC 7 cut(s) 1780, 2000, 2423, 2568, 2808, 2829, 2983
BssSI CACGAG 3 cut(s) 165, 2105, 2207
BssT1I CCWWGG 1 cut(s) 150
Bst2BI CACGAG 3 cut(s) 165, 2105, 2207
Bst2UI CCWGG 3 cut(s) 1521, 1794, 2720
Bst4CI ACNGT 8 cut(s) 71, 1372, 1840, 2374, 2705, 2818, 2869, 2990
Bst6I CTCTTC 2 cut(s) 1521, 3012
BstAPI GCANNNNNTGC 2 cut(s) 131, 484
BstC8I GCNNGC 6 cut(s) 123, 689, 1415, 2413, 2899, 3027
BstDEI CTNAG 9 cut(s) 16, 815, 937, 1073, 1336, 1623, 2419, 2876, 2979
BstENI CCTNNNNNAGG 1 cut(s) 288
BstF5I GGATG 6 cut(s) 125, 306, 339, 1851, 2290, 2781
BstFNI CGCG 1 cut(s) 136
BstHHI GCGC 2 cut(s) 136, 1791
BstKTI GATC 7 cut(s) 1783, 2003, 2426, 2571, 2811, 2832, 2986
BstMAI GTCTC 7 cut(s) 367, 603, 706, 822, 830, 1136, 2422
BstMBI GATC 7 cut(s) 1780, 2000, 2423, 2568, 2808, 2829, 2983
BstMWI GCNNNNNNNGC 5 cut(s) 131, 185, 484, 902, 1673
BstNI CCWGG 3 cut(s) 1521, 1794, 2720
BstNSI RCATGY 3 cut(s) 1140, 1417, 2415
BstSCI CCNGG 3 cut(s) 1519, 1792, 2718
BstSFI CTRYAG 7 cut(s) 472, 845, 1017, 1398, 1923, 1985, 2499
BstSLI GKGCMC 1 cut(s) 2855
BstUI CGCG 1 cut(s) 136
BstV1I GCAGC 2 cut(s) 914, 2962
BstV2I GAAGAC 1 cut(s) 1820
BstX2I RGATCY 1 cut(s) 1780
BstXI CCANNNNNNTGG 2 cut(s) 157, 2880
BstYI RGATCY 1 cut(s) 1780
BsuI GTATCC 4 cut(s) 59, 214, 2416, 2467
BsuRI GGCC 7 cut(s) 261, 397, 630, 645, 1933, 2520, 3018
BtsCI GGATG 6 cut(s) 125, 306, 339, 1851, 2290, 2781
BtsI GCAGTG 4 cut(s) 456, 842, 1071, 2436
BveI ACCTGC 2 cut(s) 838, 1978
Cac8I GCNNGC 6 cut(s) 123, 689, 1415, 2413, 2899, 3027
CaiI CAGNNNCTG 4 cut(s) 1286, 2072, 2174, 2933
CciI TCATGA 1 cut(s) 2796
CfoI GCGC 2 cut(s) 136, 1791
Cfr10I RCCGGY 1 cut(s) 2660
Cfr13I GGNCC 4 cut(s) 1714, 1931, 2663, 3017
CseI GACGC 3 cut(s) 555, 2404, 2890
Csp6I GTAC 3 cut(s) 934, 1380, 1841
CspAI ACCGGT 1 cut(s) 2660
CviAII CATG 8 cut(s) 143, 964, 1137, 1414, 2412, 2513, 2572, 2797
CviQI GTAC 3 cut(s) 934, 1380, 1841
DdeI CTNAG 9 cut(s) 16, 815, 937, 1073, 1336, 1623, 2419, 2876, 2979
DpnI GATC 7 cut(s) 1782, 2002, 2425, 2570, 2810, 2831, 2985
DpnII GATC 7 cut(s) 1780, 2000, 2423, 2568, 2808, 2829, 2983
DraI TTTAAA 3 cut(s) 493, 1435, 2652
DrdI GACNNNNNNGTC 2 cut(s) 973, 1589
DriI GACNNNNNGTC 1 cut(s) 1074
DseDI GACNNNNNNGTC 2 cut(s) 973, 1589
EaeI YGGCCR 1 cut(s) 395
Eam1104I CTCTTC 2 cut(s) 1521, 3012
Eam1105I GACNNNNNGTC 1 cut(s) 1074
EarI CTCTTC 2 cut(s) 1521, 3012
Eco130I CCWWGG 1 cut(s) 150
Eco24I GRGCYC 1 cut(s) 1154
Eco31I GGTCTC 3 cut(s) 367, 603, 706
Eco32I GATATC 1 cut(s) 2995
Eco47I GGWCC 2 cut(s) 1714, 2663
Eco57I CTGAAG 2 cut(s) 408, 1761
Eco88I CYCGRG 1 cut(s) 3020
EcoNI CCTNNNNNAGG 1 cut(s) 288
EcoRII CCWGG 3 cut(s) 1519, 1792, 2718
EcoRV GATATC 1 cut(s) 2995
EcoT14I CCWWGG 1 cut(s) 150
EcoT22I ATGCAT 1 cut(s) 991
EcoT38I GRGCYC 1 cut(s) 1154
ErhI CCWWGG 1 cut(s) 150
Esp3I CGTCTC 2 cut(s) 1136, 2422
FaeI CATG 8 cut(s) 146, 967, 1140, 1417, 2415, 2516, 2575, 2800
FalI AAGNNNNNCTT 2 cut(s) 1500, 1532
FaqI GGGAC 3 cut(s) 810, 2095, 2197
FatI CATG 8 cut(s) 142, 963, 1136, 1413, 2411, 2512, 2571, 2796
FauNDI CATATG 1 cut(s) 1203
Fnu4HI GCNGC 2 cut(s) 903, 2976
FokI GGATG 6 cut(s) 112, 313, 326, 1858, 2297, 2788
FriOI GRGCYC 1 cut(s) 1154
Fsp4HI GCNGC 2 cut(s) 903, 2976
FspBI CTAG 5 cut(s) 185, 537, 764, 1158, 1722
GlaI GCGC 2 cut(s) 135, 1790
GluI GCNGC 2 cut(s) 903, 2976
GsaI CCCAGC 1 cut(s) 1833
HaeIII GGCC 7 cut(s) 261, 397, 630, 645, 1933, 2520, 3018
HapII CCGG 2 cut(s) 2241, 2661
HgaI GACGC 3 cut(s) 555, 2404, 2890
HhaI GCGC 2 cut(s) 136, 1791
Hin1II CATG 8 cut(s) 146, 967, 1140, 1417, 2415, 2516, 2575, 2800
Hin6I GCGC 2 cut(s) 134, 1789
HinP1I GCGC 2 cut(s) 134, 1789
HincII GTYRAC 2 cut(s) 943, 2370
HindII GTYRAC 2 cut(s) 943, 2370
HindIII AAGCTT 2 cut(s) 670, 1674
HinfI GANTC 5 cut(s) 526, 665, 1075, 2121, 2223
HpaI GTTAAC 2 cut(s) 943, 2370
HpaII CCGG 2 cut(s) 2241, 2661
Hpy166II GTNNAC 4 cut(s) 943, 2370, 2461, 2924
Hpy8I GTNNAC 4 cut(s) 943, 2370, 2461, 2924
HpyCH4III ACNGT 8 cut(s) 71, 1372, 1840, 2374, 2705, 2818, 2869, 2990
HpyCH4IV ACGT 1 cut(s) 2644
HpyF10VI GCNNNNNNNGC 5 cut(s) 131, 185, 484, 902, 1673
HpyF3I CTNAG 9 cut(s) 16, 815, 937, 1073, 1336, 1623, 2419, 2876, 2979
HpySE526I ACGT 1 cut(s) 2644
Hsp92II CATG 8 cut(s) 146, 967, 1140, 1417, 2415, 2516, 2575, 2800
HspAI GCGC 2 cut(s) 134, 1789
KspAI GTTAAC 2 cut(s) 943, 2370
Kzo9I GATC 7 cut(s) 1780, 2000, 2423, 2568, 2808, 2829, 2983
LguI GCTCTTC 1 cut(s) 1521
LmnI GCTCC 6 cut(s) 1553, 1627, 2098, 2200, 2434, 2912
Lsp1109I GCAGC 2 cut(s) 914, 2962
MaeI CTAG 5 cut(s) 185, 537, 764, 1158, 1722
MaeII ACGT 1 cut(s) 2644
MalI GATC 7 cut(s) 1782, 2002, 2425, 2570, 2810, 2831, 2985
MboI GATC 7 cut(s) 1780, 2000, 2423, 2568, 2808, 2829, 2983
MboII GAAGA 7 cut(s) 1367, 1508, 1754, 1825, 1922, 2999, 3002
MfeI CAATTG 1 cut(s) 603
MflI RGATCY 1 cut(s) 1780
MhlI GDGCHC 2 cut(s) 1154, 2855
MlsI TGGCCA 1 cut(s) 397
MluNI TGGCCA 1 cut(s) 397
MlyI GAGTC 3 cut(s) 1084, 2115, 2217
MmeI TCCRAC 3 cut(s) 562, 1409, 2521
Mox20I TGGCCA 1 cut(s) 397
Mph1103I ATGCAT 1 cut(s) 991
MroXI GAANNNNTTC 1 cut(s) 3006
MscI TGGCCA 1 cut(s) 397
MslI CAYNNNNRTG 3 cut(s) 344, 2027, 2801
Msp20I TGGCCA 1 cut(s) 397
MspA1I CMGCKG 3 cut(s) 47, 623, 905
MspI CCGG 2 cut(s) 2241, 2661
MspR9I CCNGG 3 cut(s) 1521, 1794, 2720
MunI CAATTG 1 cut(s) 603
Mva1269I GAATGC 2 cut(s) 1541, 2949
MvaI CCWGG 3 cut(s) 1521, 1794, 2720
MvnI CGCG 1 cut(s) 136
MwoI GCNNNNNNNGC 5 cut(s) 131, 185, 484, 902, 1673
NdeI CATATG 1 cut(s) 1203
NdeII GATC 7 cut(s) 1780, 2000, 2423, 2568, 2808, 2829, 2983
NlaIII CATG 8 cut(s) 146, 967, 1140, 1417, 2415, 2516, 2575, 2800
NlaIV GGNNCC 6 cut(s) 733, 1186, 1716, 1782, 2914, 3019
NmuCI GTSAC 8 cut(s) 454, 467, 907, 1066, 1106, 1229, 1580, 1849
NsiI ATGCAT 1 cut(s) 991
NspI RCATGY 3 cut(s) 1140, 1417, 2415
OliI CACNNNNGTG 1 cut(s) 2027
PaeI GCATGC 2 cut(s) 1417, 2415
PagI TCATGA 1 cut(s) 2796
PaqCI CACCTGC 1 cut(s) 838
PciSI GCTCTTC 1 cut(s) 1521
PctI GAATGC 2 cut(s) 1541, 2949
PdmI GAANNNNTTC 1 cut(s) 3006
PfeI GAWTC 2 cut(s) 526, 665
PflMI CCANNNNNTGG 2 cut(s) 626, 2540
PinAI ACCGGT 1 cut(s) 2660
PkrI GCNGC 2 cut(s) 904, 2977
PleI GAGTC 3 cut(s) 1083, 2115, 2217
PpsI GAGTC 3 cut(s) 1083, 2115, 2217
PshBI ATTAAT 1 cut(s) 1757
PsiI TTATAA 1 cut(s) 1299
Psp6I CCWGG 3 cut(s) 1519, 1792, 2718
PspFI CCCAGC 1 cut(s) 1829
PspGI CCWGG 3 cut(s) 1519, 1792, 2718
PspN4I GGNNCC 6 cut(s) 733, 1186, 1716, 1782, 2914, 3019
PspPI GGNCC 4 cut(s) 1714, 1931, 2663, 3017
PsrI GAACNNNNNNTAC 2 cut(s) 130, 162
PstI CTGCAG 3 cut(s) 849, 1021, 1989
PstNI CAGNNNCTG 4 cut(s) 1286, 2072, 2174, 2933
PsuI RGATCY 1 cut(s) 1780
PvuII CAGCTG 3 cut(s) 47, 623, 905
RsaI GTAC 3 cut(s) 935, 1381, 1842
RsaNI GTAC 3 cut(s) 934, 1380, 1841
RseI CAYNNNNRTG 3 cut(s) 344, 2027, 2801
SapI GCTCTTC 1 cut(s) 1521
SatI GCNGC 2 cut(s) 903, 2976
Sau3AI GATC 7 cut(s) 1780, 2000, 2423, 2568, 2808, 2829, 2983
Sau96I GGNCC 4 cut(s) 1714, 1931, 2663, 3017
SchI GAGTC 3 cut(s) 1084, 2115, 2217
ScrFI CCNGG 3 cut(s) 1521, 1794, 2720
SduI GDGCHC 2 cut(s) 1154, 2855
SfcI CTRYAG 7 cut(s) 472, 845, 1017, 1398, 1923, 1985, 2499
SinI GGWCC 2 cut(s) 1714, 2663
SmiMI CAYNNNNRTG 3 cut(s) 344, 2027, 2801
SmlI CTYRAG 1 cut(s) 2684
SmoI CTYRAG 1 cut(s) 2684
SphI GCATGC 2 cut(s) 1417, 2415
SsiI CCGC 3 cut(s) 1712, 2409, 2510
SspI AATATT 4 cut(s) 429, 2380, 2551, 2584
SspMI CTAG 5 cut(s) 185, 537, 764, 1158, 1722
StyD4I CCNGG 3 cut(s) 1519, 1792, 2718
StyI CCWWGG 1 cut(s) 150
TaaI ACNGT 8 cut(s) 71, 1372, 1840, 2374, 2705, 2818, 2869, 2990
TaiI ACGT 1 cut(s) 2647
TaqI TCGA 2 cut(s) 1470, 1537
TaqII GACCGA 1 cut(s) 2579
TfiI GAWTC 2 cut(s) 526, 665
TseFI GTSAC 8 cut(s) 454, 467, 907, 1066, 1106, 1229, 1580, 1849
TseI GCWGC 2 cut(s) 902, 2975
Tsp45I GTSAC 8 cut(s) 454, 467, 907, 1066, 1106, 1229, 1580, 1849
TspDTI ATGAA 8 cut(s) 584, 678, 995, 1106, 2540, 2548, 2683, 2785
TspGWI ACGGA 1 cut(s) 1858
Van91I CCANNNNNTGG 2 cut(s) 626, 2540
VpaK11BI GGWCC 2 cut(s) 1714, 2663
VspI ATTAAT 1 cut(s) 1757
XagI CCTNNNNNAGG 1 cut(s) 288
XapI RAATTY 3 cut(s) 1406, 2555, 2843
XceI RCATGY 3 cut(s) 1140, 1417, 2415
XmnI GAANNNNTTC 1 cut(s) 3006
XspI CTAG 5 cut(s) 185, 537, 764, 1158, 1722
Zsp2I ATGCAT 1 cut(s) 991
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.