Basic Information

Type: gene

Biological Identity

Species

rosa_multiflora

Chromosome

Rmu_sc0004020.1

Orthogroup ID

OG0014465

Physical Location & Seq

Genomic Coordinates Forward (+)

150283 .. 157556

Gene Structure 7274 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4233 bp

atgagtcgttacgatgctaaccccttcgctgaggaagatgttaatccctttgcggatggtaaaggtctttcaccccttccccctgaacgctatgatcgcggtgcaacgattgatataccacttgataattctagggtaggtcggcctcttgttgattttttcagatctcatagtggtatggttgataacctctcaggttctgtagctaattttactgtagacagatcatgggttttttctccattgctccagcttcatttccctgctttgtgtgagctggtcagccaagttcccattgctttggacccatccacgacagacactttggtatggtcctcttcctcatttggcaagcttactgcaaaagttgcttttcactttttgcgccagccttatccttcagttgattggggaaggtgtatatggtcaaaatttattttgccaagaatgtcgttgttgacttggaaggttcttcgtggtcgagtactttgtgacgattttattcaaaaaagaggtattgccttagcttctagatgtgtactatgtcggaaggattgtgaatctttgagtcacatttttagaagttgtccttttgctgaaaccatttggagctgcatgattgcaaagtttgagcttgctgctcccccattgtctttatatgacatgcttcagttgggattgactaatggtcgcagccctcaattaaaggacttatgggtagcatgcttcacttcagtgctttggttcctttggcatgctagaaacatggccaaatatgacaatagatttattactgtagtgggggtgtgcaggctagtctttggacatattcaatctgctagccgtattgcttcaggccacatgcacaattcaattcaagatctccgaattttgaaatgttttggtgtgacatgtcgtccacgtcatgctccacgaacaatagaggtgaattggcatcctccttttttggggtggatcaagatcaactcagatggagcttgcatgattcggtgcgtggcgggtatggtgcggtttttcgggattacagaggtcatgttcttgatgcttttgcttctaattttgacatcccaagctcggttacagcagaggttatggcgagctggaaaaccgcaaagtcccaaagattcaaactttaaaggcagggagaagatgcttcactgccagagctacaattccagagaattagggcaagaaagtctccacagctcaatgcaaggccacaccttttcaccaagtaagcttcaaaacccccaatctaatttcactactaggcctcttctagggtttcgtttcactccccacaaccatagcttagctaaaacccaactatgccctgttccaatttctcaaaagggtagctgtattgtgggttcacgacctcagaagcagagttcaagagggtctagggtttatgtagggtttaagcttcagtgtcattcgaaagcattggttttgcccacgaaggtttctttagttaatggtaagaagaagaggtatggaggcgtgttgccttcgattttgaggtctttggagtctgaaaacgatgttgaaaaggcactcaagtcgtttggggagagtctgagtgcaaaagaacagactgtgattctcaaagaacagaggagctgggagagagttcttcgtgttttcgagtggttcaagtcacagaaagagtatgtgcccaatgtgattcactacaatgtggtgcttcgggtgcttggtagagctcagagatgggatgagctgaggctttgttgggttgaaatggcgaaaaagggtgtcctgccgactaacaatacgtattccatgcttgttgatgtgtatgggaaagcgggacttgtgaaggaagccctgttgtggattaagcacatgaagctaaggggacttttcccggatgaggttacaatgaatacggttgttcgggcattgaagaatgcagaagagtttgatagggcggataagttttataaggactggtgtactgggcggattgagctcgatgaccttgagttggattctatggctgattctgtgattggttctgtttcggaacctattagtttcaagcatttcttgtccactgagctattcaggactggtgggagagttcccacttcaaaaattatgacctcaatggatacagagaactctattcagaaaccacgacttacatctacctataatagtttgattgatttgtatggaaaggcagggcgtctgaatgatgcggctaatgtgtttggaaatatgatgaagtctggagtggcaatggatgttattacttttaatactatgatctttacttgtggaagtcatgggcatttgttggaggcggaagctttgcttagtaagatggaagaaaggggtatatctcctgatacaagaacttataacatttttctatctctgtatgctgatgtggggaacattgatgctgctctgaactgctacaggaagatcagagaggtaggtctttacccggatattgtatctcacaggaccattcttcatgtattatgtgagagaaacatggtcagagatgtggagattgtgattgaggatatggagaaatctggtgtctctattaatgagcattctcttcctggtatcatcaagatgtatattaatgaaggacggcttgatcaggcaaacatactttatgagaaatgtcagttaaaccctgggatttcatcaaagacgcgtgcagcaattatagatgcatatgctgaaaagggactttggactgaagccgaggtcgtattctataggaagggagatttggttggacagacgaaggatattgtggaatacaatgttatgatcaaagcgtatggtaaggcaaggctttatgacaaagctttctctctcttcaggggcatgaaaaagcatgggacttggcccgatgagtgcacttacaactctctcatccaaatgttctccggaggtgatttagtggaccaagcaagggatcttctaacagaaatgcaggaaactggtctgaagcctcaatctctaaccctttctgctcttattgcctgctatgctggccttggccagctttcggatgcagttgatgtgtaccaagactttgtaaagtcaggaacaaagccaaatgagtttgtgtacggttctttaatcaatggattcgcagaaactggcagagttgaagaagcacttaagtattttcaccacatggaagaatcggggataactgcaaatcaaatagttctgacatctctgataaaggcgtatagtaaggcgggaagccgtgatggagcagaagtgttgtatgagaggttgaaaggttttgatggtggcccagatgttgttgcatctaacagtatgataaatctgtatgcagatcttgggttggtatctgaagccaaattgatttttgaaaatttgagagcaaaaggttgggctgatgagatagcgtttgcaaccatgatgtatctgtacaagagcatgggcatgcttgatgaggccattgatgtagctgaggaaatgaaagaatcgggtttaataagggactgcgcttcatttaacaaggtgatgtcatgctatgccattaacgggcagctacgtgaatgcggtgaattattgcacgagatggtaactcggaaactgttgcttgacagtggaacttgtaatgtattgttcactgtattgagaaaaggagggattcccattgaagctgtaacccagctagagtcatcttaccaggaagggaaaccttactctagacaagctatcatcacctctgtgttctctctggttggtatgcatggtttggcactcgaatcctgtgaaacattcacaaaagctgacataaatcttgattcttttctctacaatgttgcgatatatgcttatggggcagctggggagattgacaaggctctgaacattttcatgagaatgcaggatgaaggtgtggaaccagaccttgtaacgcatattttcctagtaggttgttatggaaaagcaggtatggttgaaggagtgaagagaatttacagccagctgaaatatgaagagatagagcctaatccatccttgttcagagctgttatagatgcctatacagatgccaataggcatgaccttgccaaattggttaagcaagacaggaaatatgcatttgactcggaacatcatgtagattcggaaaccaaagatgagtttgatgacactaattcagagtttgaggattga

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_2 PPR_3

Protein Analysis

1410

Amino Acids

158.77

Weight (kDa)

7.49

Isoelectric Point (pI)

40.77

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-RVT	PF13966	121 - 203	1.3e-19	zinc-binding in reverse transcriptase
PPR_2	PF13041	567 - 614	1.7e-07	PPR repeat family
PPR_long	PF17177	730 - 894	1.1e-08	Pentacotripeptide-repeat region of PRORP
PPR_2	PF13041	731 - 775	4.9e-11	PPR repeat family
PPR	PF01535	731 - 761	2.5e-06	PPR repeat
PPR_2	PF13041	764 - 810	1.1e-11	PPR repeat family
PPR_3	PF13812	787 - 845	1.4e-07	Pentatricopeptide repeat domain
PPR_2	PF13041	802 - 846	2.8e-06	PPR repeat family
PPR_2	PF13041	938 - 985	1.1e-12	PPR repeat family
PPR_3	PF13812	939 - 986	3.6e-07	Pentatricopeptide repeat domain
TPR_24	PF23276	941 - 1036	6.9e-09	Fungal tetratrico peptide repeats
PPR	PF01535	943 - 970	5.6e-07	PPR repeat
PPR_long	PF17177	956 - 1069	3.9e-09	Pentacotripeptide-repeat region of PRORP
PPR_2	PF13041	973 - 1020	1.8e-07	PPR repeat family
PPR_2	PF13041	1009 - 1056	2.3e-07	PPR repeat family
PPR_1	PF12854	1040 - 1072	5e-06	PPR repeat
PPR_2	PF13041	1047 - 1092	2.8e-07	PPR repeat family
PPR	PF01535	1047 - 1076	1.2e-06	PPR repeat
PPR_3	PF13812	1280 - 1334	9.2e-06	Pentatricopeptide repeat domain
PPR_2	PF13041	1286 - 1332	2.7e-07	PPR repeat family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0014465)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G73710
fragaria_vesca	FvH4_6g44930
malus_domestica	MD09G1091700.v1.1 MD17G1081600.v1.1
prunus_persica	Prupe.3G233300_v2.0.a1
pyrus_communis	pycom09g01730 pycom17g07990
rosa_chinensis	RchiOBHm_Chr2g0162121
rosa_laevigata	RLG00000021324
rosa_multiflora	Rmu_sc0004020.1_g000031
rosa_roxburghii	Rroxscaffold_2G00088280
rosa_rugosa	Rorug02G0494800
rosa_samantha	Rh2AG561300 Rh2BG574400 Rh2CG544500 Rh2DG584300
rosa_wichuraiana	Rw2G046480

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1986, 2409
Acc36I	ACCTGC	1 cut(s)	3995
AccI	GTMKAC	1 cut(s)	219
AccII	CGCG	2 cut(s)	99, 2719
AccIII	TCCGGA	1 cut(s)	2957
AclWI	GGATC	2 cut(s)	983, 2994
AcoI	YGGCCR	2 cut(s)	768, 3070
AcsI	RAATTY	4 cut(s)	431, 888, 3418, 4029
AcuI	CTGAAG	9 cut(s)	384, 653, 717, 837, 1433, 2784, 2872, 3038, 3417
AcyI	GRCGYC	1 cut(s)	2233
AfaI	GTAC	6 cut(s)	486, 540, 1999, 3098, 3143, 3476
AfiI	CCNNNNNNNGG	9 cut(s)	968, 1095, 1301, 1875, 1915, 2029, 2983, 3079, 3591
AflII	CTTAAG	1 cut(s)	3194
AflIII	ACRYGT	2 cut(s)	911, 2717
AhdI	GACNNNNNGTC	2 cut(s)	687, 915
AjiI	CACGTC	1 cut(s)	923
AjnI	CCWGG	3 cut(s)	2620, 2698, 3738
AjuI	GAANNNNNNNTTGG	2 cut(s)	2780, 2812
AleI	CACNNNNGTG	2 cut(s)	734, 3779
Alw21I	GWGCWC	3 cut(s)	1747, 2016, 2930
Alw26I	GTCTC	2 cut(s)	1223, 2602
Alw44I	GTGCAC	1 cut(s)	2926
AlwI	GGATC	2 cut(s)	983, 2994
AlwNI	CAGNNNCTG	2 cut(s)	200, 3173
Aor13HI	TCCGGA	1 cut(s)	2957
ApaLI	GTGCAC	1 cut(s)	2926
ApeKI	GCWGC	7 cut(s)	612, 638, 693, 2453, 2723, 3595, 3896
ApoI	RAATTY	4 cut(s)	431, 888, 3418, 4029
ArsI	GACNNNNNNTTYG	2 cut(s)	307, 339
AseI	ATTAAT	2 cut(s)	2604, 2643
Asp700I	GAANNNNTTC	1 cut(s)	3926
AspLEI	GCGC	2 cut(s)	387, 3554
AspS9I	GGNCC	6 cut(s)	304, 333, 2517, 2916, 2974, 3335
AsuC2I	CCSGG	2 cut(s)	1910, 2498
AsuHPI	GGTGA	8 cut(s)	63, 958, 1242, 2975, 3197, 3580, 3623, 3766
AsuII	TTCGAA	1 cut(s)	1460
AsuNHI	GCTAGC	1 cut(s)	839
AvaII	GGWCC	4 cut(s)	304, 333, 2517, 2974
BaeGI	GKGCMC	2 cut(s)	1701, 2930
BalI	TGGCCA	2 cut(s)	770, 3072
BanII	GRGCYC	2 cut(s)	1747, 2016
BarI	GAAGNNNNNNTAC	2 cut(s)	4016, 4048
BauI	CACGAG	1 cut(s)	3623
Bbv12I	GWGCWC	3 cut(s)	1747, 2016, 2930
BbvCI	CCTCAGC	3 cut(s)	30, 1763, 3516
BbvI	GCAGC	7 cut(s)	599, 625, 705, 2440, 2735, 3607, 3908
BccI	CCATC	9 cut(s)	50, 316, 986, 1746, 2365, 3284, 3323, 3622, 4078
BceAI	ACGGC	3 cut(s)	828, 2669, 3270
BcgI	CGANNNNNNTGC	4 cut(s)	1566, 1600, 4146, 4180
BciT130I	CCWGG	3 cut(s)	2622, 2700, 3740
BciVI	GTATCC	1 cut(s)	2149
BclI	TGATCA	2 cut(s)	2659, 2838
BcnI	CCSGG	2 cut(s)	1910, 2498
BcoDI	GTCTC	2 cut(s)	1223, 2602
BfmI	CTRYAG	5 cut(s)	201, 216, 795, 2467, 2782
BfrI	CTTAAG	1 cut(s)	3194
BfuAI	ACCTGC	1 cut(s)	3995
BfuI	GTATCC	1 cut(s)	2149
BglII	AGATCT	3 cut(s)	164, 880, 3379
BisI	GCNGC	8 cut(s)	613, 639, 694, 2247, 2454, 2724, 3596, 3897
BlpI	GCTNAGC	1 cut(s)	1333
BlsI	GCNGC	8 cut(s)	614, 640, 695, 2248, 2455, 2725, 3597, 3898
BmcAI	AGTACT	1 cut(s)	486
Bme1390I	CCNGG	5 cut(s)	1910, 2498, 2622, 2700, 3740
Bme18I	GGWCC	4 cut(s)	304, 333, 2517, 2974
BmeRI	GACNNNNNGTC	2 cut(s)	687, 915
BmgBI	CACGTC	1 cut(s)	923
BmgT120I	GGNCC	6 cut(s)	304, 333, 2517, 2916, 2974, 3335
BmiI	GGNNCC	4 cut(s)	306, 746, 2070, 3957
BmrFI	CCNGG	5 cut(s)	1910, 2498, 2622, 2700, 3740
BmrI	ACTGGG	1 cut(s)	2010
BmtI	GCTAGC	1 cut(s)	843
BmuI	ACTGGG	1 cut(s)	2010
BpmI	CTGGAG	2 cut(s)	233, 2298
Bpu10I	CCTNAGC	5 cut(s)	30, 523, 1763, 1895, 3516
Bpu1102I	GCTNAGC	1 cut(s)	1333
Bpu14I	TTCGAA	1 cut(s)	1460
BpuEI	CTTGAG	2 cut(s)	1565, 2045
BpuMI	CCSGG	2 cut(s)	1910, 2498
BsaAI	YACGTR	2 cut(s)	1818, 3602
BsaBI	GATNNNNATC	2 cut(s)	42, 980
BsaHI	GRCGYC	1 cut(s)	2233
BsaJI	CCNNGG	4 cut(s)	2698, 2699, 2769, 3067
BsaWI	WCCGGW	1 cut(s)	2957
BsaXI	ACNNNNNCTCC	4 cut(s)	2784, 2814, 3285, 3315
Bsc4I	CCNNNNNNNGG	9 cut(s)	968, 1095, 1301, 1875, 1915, 2029, 2983, 3079, 3591
Bse1I	ACTGG	5 cut(s)	1997, 2005, 2119, 3016, 3178
Bse3DI	GCAATG	3 cut(s)	242, 294, 2292
Bse8I	GATNNNNATC	2 cut(s)	42, 980
BseAI	TCCGGA	1 cut(s)	2957
BseBI	CCWGG	3 cut(s)	2622, 2700, 3740
BseDI	CCNNGG	4 cut(s)	2698, 2699, 2769, 3067
BseJI	GATNNNNATC	2 cut(s)	42, 980
BseLI	CCNNNNNNNGG	9 cut(s)	968, 1095, 1301, 1875, 1915, 2029, 2983, 3079, 3591
BseMI	GCAATG	3 cut(s)	242, 294, 2292
BseMII	CTCAG	9 cut(s)	21, 207, 1002, 1415, 1592, 1754, 1760, 2091, 3507
BseNI	ACTGG	5 cut(s)	1997, 2005, 2119, 3016, 3178
BseRI	GAGGAG	1 cut(s)	1654
BseSI	GKGCMC	2 cut(s)	1701, 2930
BseXI	GCAGC	7 cut(s)	599, 625, 705, 2440, 2735, 3607, 3908
BseYI	CCCAGC	3 cut(s)	1644, 3720, 3899
BsgI	GTGCAG	2 cut(s)	829, 2742
Bsh1236I	CGCG	2 cut(s)	99, 2719
BsiHKAI	GWGCWC	3 cut(s)	1747, 2016, 2930
BsiSI	CCGG	3 cut(s)	1910, 2498, 2958
BslFI	GGGAC	6 cut(s)	1122, 1866, 1914, 2766, 2923, 3560
BslI	CCNNNNNNNGG	9 cut(s)	968, 1095, 1301, 1875, 1915, 2029, 2983, 3079, 3591
BsmAI	GTCTC	2 cut(s)	1223, 2602
BsmFI	GGGAC	6 cut(s)	1122, 1866, 1914, 2766, 2923, 3560
BsmI	GAATGC	4 cut(s)	1957, 2611, 3611, 3942
Bsp119I	TTCGAA	1 cut(s)	1460
Bsp1286I	GDGCHC	4 cut(s)	1701, 1747, 2016, 2930
Bsp13I	TCCGGA	1 cut(s)	2957
Bsp1407I	TGTACA	1 cut(s)	3474
Bsp1720I	GCTNAGC	1 cut(s)	1333
BspCNI	CTCAG	9 cut(s)	22, 206, 1001, 1414, 1593, 1755, 1759, 2092, 3508
BspEI	TCCGGA	1 cut(s)	2957
BspFNI	CGCG	2 cut(s)	99, 2719
BspHI	TCATGA	1 cut(s)	3930
BspLI	GGNNCC	4 cut(s)	306, 746, 2070, 3957
BspMI	ACCTGC	1 cut(s)	3995
BspOI	GCTAGC	1 cut(s)	843
BspPI	GGATC	2 cut(s)	983, 2994
BspT104I	TTCGAA	1 cut(s)	1460
BspTI	CTTAAG	1 cut(s)	3194
BsrDI	GCAATG	3 cut(s)	242, 294, 2292
BsrGI	TGTACA	1 cut(s)	3474
BsrI	ACTGG	5 cut(s)	1997, 2005, 2119, 3016, 3178
BssECI	CCNNGG	4 cut(s)	2698, 2699, 2769, 3067
BssNI	GRCGYC	1 cut(s)	2233
BssSI	CACGAG	1 cut(s)	3623
BssT1I	CCWWGG	1 cut(s)	3067
Bst2BI	CACGAG	1 cut(s)	3623
Bst2UI	CCWGG	3 cut(s)	2622, 2700, 3740
Bst4CI	ACNGT	9 cut(s)	217, 796, 1621, 1933, 3146, 3359, 3645, 3656, 3682
Bst6I	CTCTTC	8 cut(s)	343, 1302, 1505, 1953, 2622, 2891, 4019, 4047
BstACI	GRCGYC	1 cut(s)	2233
BstAFI	CTTAAG	1 cut(s)	3194
BstAPI	GCANNNNNTGC	1 cut(s)	368
BstAUI	TGTACA	1 cut(s)	3474
BstBAI	YACGTR	2 cut(s)	1818, 3602
BstBI	TTCGAA	1 cut(s)	1460
BstENI	CCTNNNNNAGG	1 cut(s)	1299
BstFNI	CGCG	2 cut(s)	99, 2719
BstHHI	GCGC	2 cut(s)	387, 3554
BstMAI	GTCTC	2 cut(s)	1223, 2602
BstNI	CCWGG	3 cut(s)	2622, 2700, 3740
BstNSI	RCATGY	6 cut(s)	667, 726, 758, 865, 915, 3493
BstSCI	CCNGG	5 cut(s)	1908, 2496, 2620, 2698, 3738
BstSFI	CTRYAG	5 cut(s)	201, 216, 795, 2467, 2782
BstSLI	GKGCMC	2 cut(s)	1701, 2930
BstSNI	TACGTA	1 cut(s)	1818
BstUI	CGCG	2 cut(s)	99, 2719
BstV1I	GCAGC	7 cut(s)	599, 625, 705, 2440, 2735, 3607, 3908
BstX2I	RGATCY	4 cut(s)	164, 880, 2986, 3379
BstXI	CCANNNNNNTGG	1 cut(s)	301
BstYI	RGATCY	4 cut(s)	164, 880, 2986, 3379
BsuI	GTATCC	1 cut(s)	2149
BtrI	CACGTC	1 cut(s)	923
BtsI	GCAGTG	1 cut(s)	1177
BtsIMutI	CAGTG	6 cut(s)	741, 1177, 1457, 2097, 3661, 3678
BveI	ACCTGC	1 cut(s)	3995
CaiI	CAGNNNCTG	2 cut(s)	200, 3173
CciI	TCATGA	1 cut(s)	3930
CfoI	GCGC	2 cut(s)	387, 3554
Cfr13I	GGNCC	6 cut(s)	304, 333, 2517, 2916, 2974, 3335
CseI	GACGC	2 cut(s)	2222, 2725
Csp6I	GTAC	6 cut(s)	485, 539, 1998, 3097, 3142, 3475
CviQI	GTAC	6 cut(s)	485, 539, 1998, 3097, 3142, 3475
DraI	TTTAAA	1 cut(s)	1156
DriI	GACNNNNNGTC	2 cut(s)	687, 915
EaeI	YGGCCR	2 cut(s)	768, 3070
Eam1104I	CTCTTC	8 cut(s)	343, 1302, 1505, 1953, 2622, 2891, 4019, 4047
Eam1105I	GACNNNNNGTC	2 cut(s)	687, 915
EarI	CTCTTC	8 cut(s)	343, 1302, 1505, 1953, 2622, 2891, 4019, 4047
EciI	GGCGGA	3 cut(s)	1988, 2020, 2366
Ecl136II	GAGCTC	2 cut(s)	1745, 2014
Eco105I	TACGTA	1 cut(s)	1818
Eco130I	CCWWGG	1 cut(s)	3067
Eco147I	AGGCCT	1 cut(s)	1294
Eco24I	GRGCYC	2 cut(s)	1747, 2016
Eco47I	GGWCC	4 cut(s)	304, 333, 2517, 2974
Eco53kI	GAGCTC	2 cut(s)	1745, 2014
Eco57I	CTGAAG	9 cut(s)	384, 653, 717, 837, 1433, 2784, 2872, 3038, 3417
EcoICRI	GAGCTC	2 cut(s)	1745, 2014
EcoNI	CCTNNNNNAGG	1 cut(s)	1299
EcoRII	CCWGG	3 cut(s)	2620, 2698, 3738
EcoT14I	CCWWGG	1 cut(s)	3067
EcoT22I	ATGCAT	3 cut(s)	2740, 3804, 4159
EcoT38I	GRGCYC	2 cut(s)	1747, 2016
ErhI	CCWWGG	1 cut(s)	3067
FaqI	GGGAC	6 cut(s)	1122, 1866, 1914, 2766, 2923, 3560
FauI	CCCGC	3 cut(s)	1012, 1843, 3271
FauNDI	CATATG	1 cut(s)	2740
FbaI	TGATCA	2 cut(s)	2659, 2838
FblI	GTMKAC	1 cut(s)	219
Fnu4HI	GCNGC	8 cut(s)	613, 639, 694, 2247, 2454, 2724, 3596, 3897
FriOI	GRGCYC	2 cut(s)	1747, 2016
Fsp4HI	GCNGC	8 cut(s)	613, 639, 694, 2247, 2454, 2724, 3596, 3897
GlaI	GCGC	2 cut(s)	386, 3553
GluI	GCNGC	8 cut(s)	613, 639, 694, 2247, 2454, 2724, 3596, 3897
GsaI	CCCAGC	3 cut(s)	1648, 3724, 3903
GsuI	CTGGAG	2 cut(s)	233, 2298
HapII	CCGG	3 cut(s)	1910, 2498, 2958
HgaI	GACGC	2 cut(s)	2222, 2725
HhaI	GCGC	2 cut(s)	387, 3554
Hin1I	GRCGYC	1 cut(s)	2233
Hin6I	GCGC	2 cut(s)	385, 3552
HinP1I	GCGC	2 cut(s)	385, 3552
HincII	GTYRAC	1 cut(s)	459
HindII	GTYRAC	1 cut(s)	459
HindIII	AAGCTT	5 cut(s)	353, 1259, 1445, 2355, 2874
HpaII	CCGG	3 cut(s)	1910, 2498, 2958
HphI	GGTGA	8 cut(s)	63, 958, 1242, 2975, 3197, 3580, 3623, 3766
HpyCH4III	ACNGT	9 cut(s)	217, 796, 1621, 1933, 3146, 3359, 3645, 3656, 3682
HpyCH4IV	ACGT	3 cut(s)	922, 1817, 3601
HpySE526I	ACGT	3 cut(s)	922, 1817, 3601
Hsp92I	GRCGYC	1 cut(s)	2233
HspAI	GCGC	2 cut(s)	385, 3552
Kpn2I	TCCGGA	1 cut(s)	2957
Ksp22I	TGATCA	2 cut(s)	2659, 2838
LmnI	GCTCC	7 cut(s)	252, 609, 646, 934, 995, 1641, 3293
Lsp1109I	GCAGC	7 cut(s)	599, 625, 705, 2440, 2735, 3607, 3908
MaeII	ACGT	3 cut(s)	922, 1817, 3601
MflI	RGATCY	4 cut(s)	164, 880, 2986, 3379
MhlI	GDGCHC	4 cut(s)	1701, 1747, 2016, 2930
MlsI	TGGCCA	2 cut(s)	770, 3072
MluI	ACGCGT	1 cut(s)	2717
MluNI	TGGCCA	2 cut(s)	770, 3072
MlyI	GAGTC	6 cut(s)	13, 577, 1562, 1606, 3737, 4157
MmeI	TCCRAC	4 cut(s)	527, 2010, 2325, 2782
Mox20I	TGGCCA	2 cut(s)	770, 3072
Mph1103I	ATGCAT	3 cut(s)	2740, 3804, 4159
MroI	TCCGGA	1 cut(s)	2957
MroXI	GAANNNNTTC	1 cut(s)	3926
MscI	TGGCCA	2 cut(s)	770, 3072
MslI	CAYNNNNRTG	8 cut(s)	734, 1716, 2633, 2948, 3488, 3779, 3929, 3935
Msp20I	TGGCCA	2 cut(s)	770, 3072
MspA1I	CMGCKG	2 cut(s)	3899, 4042
MspCI	CTTAAG	1 cut(s)	3194
MspI	CCGG	3 cut(s)	1910, 2498, 2958
MspR9I	CCNGG	5 cut(s)	1910, 2498, 2622, 2700, 3740
Mva1269I	GAATGC	4 cut(s)	1957, 2611, 3611, 3942
MvaI	CCWGG	3 cut(s)	2622, 2700, 3740
MvnI	CGCG	2 cut(s)	99, 2719
NciI	CCSGG	2 cut(s)	1910, 2498
NdeI	CATATG	1 cut(s)	2740
NheI	GCTAGC	1 cut(s)	839
NlaIV	GGNNCC	4 cut(s)	306, 746, 2070, 3957
NmeAIII	GCCGAG	1 cut(s)	2794
NmuCI	GTSAC	4 cut(s)	491, 569, 907, 1680
NsiI	ATGCAT	3 cut(s)	2740, 3804, 4159
NspI	RCATGY	6 cut(s)	667, 726, 758, 865, 915, 3493
NspV	TTCGAA	1 cut(s)	1460
OliI	CACNNNNGTG	2 cut(s)	734, 3779
PaeI	GCATGC	3 cut(s)	726, 758, 3493
PagI	TCATGA	1 cut(s)	3930
PasI	CCCWGGG	1 cut(s)	2699
PceI	AGGCCT	1 cut(s)	1294
PciI	ACATGT	1 cut(s)	911
PctI	GAATGC	4 cut(s)	1957, 2611, 3611, 3942
PdmI	GAANNNNTTC	1 cut(s)	3926
PfoI	TCCNGGA	1 cut(s)	1908
PkrI	GCNGC	8 cut(s)	614, 640, 695, 2248, 2455, 2725, 3597, 3898
PleI	GAGTC	6 cut(s)	12, 576, 1561, 1605, 3736, 4157
PpsI	GAGTC	6 cut(s)	12, 576, 1561, 1605, 3736, 4157
Ppu21I	YACGTR	2 cut(s)	1818, 3602
PscI	ACATGT	1 cut(s)	911
PshBI	ATTAAT	2 cut(s)	2604, 2643
PsiI	TTATAA	2 cut(s)	1986, 2409
Psp124BI	GAGCTC	2 cut(s)	1747, 2016
Psp6I	CCWGG	3 cut(s)	2620, 2698, 3738
PspFI	CCCAGC	3 cut(s)	1644, 3720, 3899
PspGI	CCWGG	3 cut(s)	2620, 2698, 3738
PspN4I	GGNNCC	4 cut(s)	306, 746, 2070, 3957
PspPI	GGNCC	6 cut(s)	304, 333, 2517, 2916, 2974, 3335
PsrI	GAACNNNNNNTAC	2 cut(s)	1375, 1407
PstNI	CAGNNNCTG	2 cut(s)	200, 3173
PsuI	RGATCY	4 cut(s)	164, 880, 2986, 3379
PvuII	CAGCTG	2 cut(s)	3899, 4042
RsaI	GTAC	6 cut(s)	486, 540, 1999, 3098, 3143, 3476
RsaNI	GTAC	6 cut(s)	485, 539, 1998, 3097, 3142, 3475
RseI	CAYNNNNRTG	8 cut(s)	734, 1716, 2633, 2948, 3488, 3779, 3929, 3935
SacI	GAGCTC	2 cut(s)	1747, 2016
SatI	GCNGC	8 cut(s)	613, 639, 694, 2247, 2454, 2724, 3596, 3897
Sau96I	GGNCC	6 cut(s)	304, 333, 2517, 2916, 2974, 3335
ScaI	AGTACT	1 cut(s)	486
SchI	GAGTC	6 cut(s)	13, 577, 1562, 1606, 3737, 4157
ScrFI	CCNGG	5 cut(s)	1910, 2498, 2622, 2700, 3740
SduI	GDGCHC	4 cut(s)	1701, 1747, 2016, 2930
SfcI	CTRYAG	5 cut(s)	201, 216, 795, 2467, 2782
SfuI	TTCGAA	1 cut(s)	1460
SinI	GGWCC	4 cut(s)	304, 333, 2517, 2974
SmiMI	CAYNNNNRTG	8 cut(s)	734, 1716, 2633, 2948, 3488, 3779, 3929, 3935
SmlI	CTYRAG	3 cut(s)	1580, 2024, 3194
SmoI	CTYRAG	3 cut(s)	1580, 2024, 3194
SnaBI	TACGTA	1 cut(s)	1818
SphI	GCATGC	3 cut(s)	726, 758, 3493
SseBI	AGGCCT	1 cut(s)	1294
SstI	GAGCTC	2 cut(s)	1747, 2016
StuI	AGGCCT	1 cut(s)	1294
StyD4I	CCNGG	5 cut(s)	1908, 2496, 2620, 2698, 3738
StyI	CCWWGG	1 cut(s)	3067
TaaI	ACNGT	9 cut(s)	217, 796, 1621, 1933, 3146, 3359, 3645, 3656, 3682
TaiI	ACGT	3 cut(s)	925, 1820, 3604
TaqI	TCGA	6 cut(s)	481, 1460, 1535, 1668, 2016, 3816
TatI	WGTACW	4 cut(s)	484, 538, 1997, 3474
TauI	GCSGC	1 cut(s)	2249
TscAI	CASTG	6 cut(s)	741, 1184, 1457, 2104, 3661, 3685
TseFI	GTSAC	4 cut(s)	491, 569, 907, 1680
TseI	GCWGC	7 cut(s)	612, 638, 693, 2453, 2723, 3595, 3896
Tsp45I	GTSAC	4 cut(s)	491, 569, 907, 1680
TspRI	CASTG	6 cut(s)	741, 1184, 1457, 2104, 3661, 3685
Vha464I	CTTAAG	1 cut(s)	3194
VneI	GTGCAC	1 cut(s)	2926
VpaK11BI	GGWCC	4 cut(s)	304, 333, 2517, 2974
VspI	ATTAAT	2 cut(s)	2604, 2643
XagI	CCTNNNNNAGG	1 cut(s)	1299
XapI	RAATTY	4 cut(s)	431, 888, 3418, 4029
XbaI	TCTAGA	2 cut(s)	530, 3758
XceI	RCATGY	6 cut(s)	667, 726, 758, 865, 915, 3493
XmiI	GTMKAC	1 cut(s)	219
XmnI	GAANNNNTTC	1 cut(s)	3926
ZrmI	AGTACT	1 cut(s)	486
Zsp2I	ATGCAT	3 cut(s)	2740, 3804, 4159

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.