Basic Information

Type: gene

Biological Identity

Species

rosa_multiflora

Chromosome

Rmu_sc0004338.1

Orthogroup ID

OG0007034

Physical Location & Seq

Genomic Coordinates Forward (+)

9776 .. 16072

Gene Structure 6297 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2829 bp

atgtcagaaactggtggaaactggggttcccataagaagccggcaaaacttgcatcttcatctgctaatatgacagcagaggagttgaaattgcttctgagagaccataggtttcataacagcagaaaggatgttgcccccaatcgaagtggaagtgcacccccaagtatggagggctcatttttggccattaataatctgatatcccagcaggactctggcatgactggaagcttggctagtttaactagtgttattgaaaggtgcgagtctgaggagcaactgcgttcagatccagcttatctggcttattactgtgccaatgttaatctgaatccaagacttccttctccccttatctcatgggagaatcgacgtctggcacgccatatcggtagtttcagtccaaacttgggacccgttgatgacagtggtaatggttcattgcatatgtctccaggactactgcctacacacgaggaagagtcagaggatgatcagacacctcaacagacttctaatgattgggtagatcagacaagttctggagaggaagcagctcaattgataggacaacagaaaaatgaagattcttgtgggtctacttcacctgtagataatcaatcacatacctcaagcaatggggtacctgaggagtttactcagagtgctgtttccaattccttgcatgatctccctaggcccaatgtagatgaagaacgtgcagctgatacagtgctttcactaaatgatgaagcctcatccagtcctgttgcaatttcgtcgtctcatgtttataccaggagtacaggtgttaatgatgcagatgtctcccttgttgaatctggaatgaagtctcttaatgtatcgaacatgccagaaaatcaaaagaatcaagtacagtggccgcccagctaccagaataatttcctgcaataccagatacagcaacagcaaatcaatttatgccaagttcaaaatgccacatctcagataactcctcaaggagctcactgcacatatattggcttggatcactatcaccctaatacctccaaatttgcagcggatgcacagccggtactccagacatcagggttcgcacatccactttatgcaaacgcagcagcctacacgagtgcaacacatccgtattattcaaattttcaggcacagagctttgtttctccacaatatgtaggtggatatgctttaaacccccctggtttttctccatatgttggtggatatcatcctccaggggctgttcctgttgttgttgagggaactgtgggttcaagctttaatactcgaacatctgtgggaagcatttcacctggggctgatgtgcaacatataagcaagtcatatgggcagcctgggtttcccctgcaaacttcttattccgatcctatgtatatgcaatactatcaacagccgtttgtggagtcatatggtgtttctggccagtatgctccattggcatctagaggtggtcttgacttaaacaaagcgtccaatcatgccactagtttggatgaccacaaaatccaacatccgaggaatgtagggccggggaatttgaactcacaaagaggaccactaagtgccagctattatggaagtgcaccaaatgttggtgttttgatgcagtatcctacctcacccctaaatagtcctgttctgccaacatcatccataagttcaggaagaaatactggcttatatcatggatggacagggcaccgaggctttgagagtcttcatgaccccaaaatttataattttcttgaggagttgaaatctggcaaaggccgcagatttgagctatctgatatcacaggacatattgttgaatttagtgctgatcaacatgggagtcgatttatccagcagaagttggaaaactgtagtattgaagagaaggcttctgtattcagagaagttctcccacatgcttgtcaattaatgactgatgtttttggtaactatgtgattcaaaagttttttgagtatggaacccctcagcaaaggaaggatcttgccaatcaactttcaggccaaattttgcctctaagtctgcagatgtacggctgtcgtgtaatccaaaaggcacttgatgtgattgagcttgaacagaaagcaaggcttgtacatgagttggatggaaatgttatgagatgtgttcgtgaccaaaatggaaaccatgtcatacaaaagtgtattgagagcatcccaacagaaaagattgggtttataatatctgctttccatgatcaagttgcaacactttcaatgcatccctatggttgccgagttatacagagagttctggagcattgtacaaatgagttgcaatgccagtttatagtagatgagattttggactctgtgcatgctcttgctcaggaccagtatggcaattatgtctcccagcatgtattggggagggggaaacctcatgaaagaagccgaattattagcaagttgtcgggtcatattgtacagctgagccagcacaaatttgcatccaatgttgttgagaaatgtttggagtatggtgacgctgcagaacgagaactgttgataagggagattgttggccctaatgatggaaatgataacttattgataatgatgaaggaccaatttgctaattatgtggtgcagaaggctcttgacatctgtacagacagtcaacgaacgatattgattaatcgcataagggctcatactcatgcgttgaagaaatacacttatgggaaacacattgttgctcgatttgagcagctatttggagaagaaaatcagtcaggatcctgcagctga

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0002252 GO:0002376 GO:0003674 GO:0003676 GO:0003723 GO:0003727 GO:0003729 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005829 GO:0006417 GO:0006950 GO:0006952 GO:0006970 GO:0008150 GO:0009414 GO:0009415 GO:0009605 GO:0009607 GO:0009615 GO:0009628 GO:0009651 GO:0009819 GO:0009889 GO:0009890 GO:0009892 GO:0009893 GO:0009894 GO:0009896 GO:0010035 GO:0010468 GO:0010556 GO:0010558 GO:0010604 GO:0010605 GO:0010608 GO:0010629 GO:0017148 GO:0019219 GO:0019222 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031329 GO:0031331 GO:0032268 GO:0032269 GO:0034248 GO:0034249 GO:0042221 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043487 GO:0043488 GO:0044424 GO:0044444 GO:0044464 GO:0045935 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050779 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051173 GO:0051246 GO:0051248 GO:0051252 GO:0051254 GO:0051607 GO:0051704 GO:0051707 GO:0060255 GO:0061013 GO:0061014 GO:0061157 GO:0061158 GO:0065007 GO:0065008 GO:0080090 GO:0097159 GO:0098542 GO:1901363 GO:1901700 GO:1903311 GO:1903313 GO:2000112 GO:2000113

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DUF630 DUF632 NABP PUF

Protein Analysis

942

Amino Acids

103.76

Weight (kDa)

5.86

Isoelectric Point (pI)

46.19

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PUF	PF00806	616 - 650	1.9e-10	Pumilio-family RNA binding repeat
PUF	PF00806	652 - 685	2.5e-09	Pumilio-family RNA binding repeat
PUF	PF00806	688 - 721	4.6e-09	Pumilio-family RNA binding repeat
PUF	PF00806	760 - 795	1.7e-13	Pumilio-family RNA binding repeat
PUF	PF00806	833 - 866	6.7e-11	Pumilio-family RNA binding repeat
PUF	PF00806	879 - 908	8.6e-08	Pumilio-family RNA binding repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007034)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G25880 AT4G25880 AT4G25880 AT4G25880 AT4G25880
fragaria_vesca	FvH4_4g07600 FvH4_4g07600 FvH4_4g07600
malus_domestica	MD13G1245500.v1.1 MD16G1252800.v1.1
prunus_persica	Prupe.1G079300_v2.0.a1 Prupe.1G079300_v2.0.a1
pyrus_communis	pycom13g21640 pycom16g21410
rosa_chinensis	RchiOBHm_Chr4g0400221
rosa_laevigata	RLG00000009203
rosa_multiflora	Rmu_sc0004338.1_g000002
rosa_roxburghii	Rroxscaffold_5G00345180
rosa_rugosa	Rorug04G0023900 Rorug04G0024000
rosa_samantha	Rh4AG098900 Rh4BG097200 Rh4CG108400 Rh4DG093900
rosa_wichuraiana	Rw4G008220

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1782, 2258
AatII	GACGTC	1 cut(s)	379
Acc65I	GGTACC	1 cut(s)	646
AccB1I	GGYRCC	2 cut(s)	646, 1743
AccB7I	CCANNNNNTGG	2 cut(s)	1241, 1637
AccI	GTMKAC	1 cut(s)	602
AciI	CCGC	3 cut(s)	908, 1067, 1816
AclWI	GGATC	6 cut(s)	287, 1041, 1403, 2046, 2811, 2824
AcoI	YGGCCR	3 cut(s)	186, 905, 1465
AcsI	RAATTY	7 cut(s)	1058, 1162, 1579, 1776, 1856, 2064, 2522
AcyI	GRCGYC	1 cut(s)	376
AdeI	CACNNNGTG	1 cut(s)	1607
AfaI	GTAC	9 cut(s)	648, 808, 900, 1083, 2090, 2154, 2344, 2505, 2689
AfiI	CCNNNNNNNGG	5 cut(s)	169, 413, 1241, 1637, 2612
AhlI	ACTAGT	2 cut(s)	248, 1529
AjnI	CCWGG	6 cut(s)	457, 800, 1222, 1258, 1336, 1378
AjuI	GAANNNNNNNTTGG	2 cut(s)	331, 363
Alw21I	GWGCWC	3 cut(s)	160, 1012, 1630
Alw26I	GTCTC	6 cut(s)	96, 459, 792, 835, 861, 2434
Alw44I	GTGCAC	2 cut(s)	156, 1626
AlwI	GGATC	6 cut(s)	287, 1041, 1403, 2046, 2811, 2824
AlwNI	CAGNNNCTG	2 cut(s)	11, 1265
AoxI	GGCC	8 cut(s)	186, 701, 905, 1465, 1571, 1813, 2059, 2602
ApaLI	GTGCAC	2 cut(s)	156, 1626
ApeKI	GCWGC	9 cut(s)	557, 725, 1064, 1124, 1127, 1375, 2567, 2788, 2823
ApoI	RAATTY	7 cut(s)	1058, 1162, 1579, 1776, 1856, 2064, 2522
AseI	ATTAAT	3 cut(s)	192, 1967, 2715
Asp700I	GAANNNNTTC	2 cut(s)	926, 1330
Asp718I	GGTACC	1 cut(s)	646
AspA2I	CCTAGG	1 cut(s)	698
AspS9I	GGNCC	7 cut(s)	416, 702, 1571, 1598, 2410, 2603, 2644
AsuC2I	CCSGG	1 cut(s)	1575
AsuHPI	GGTGA	5 cut(s)	600, 1034, 1326, 1656, 2573
AvaII	GGWCC	4 cut(s)	416, 1598, 2410, 2644
AvrII	CCTAGG	1 cut(s)	698
AxyI	CCTNAGG	1 cut(s)	651
BaeGI	GKGCMC	3 cut(s)	160, 1630, 1746
BalI	TGGCCA	2 cut(s)	188, 1467
BamHI	GGATCC	1 cut(s)	2816
BanI	GGYRCC	2 cut(s)	646, 1743
BanII	GRGCYC	3 cut(s)	179, 1012, 2731
BauI	CACGAG	2 cut(s)	476, 1135
BbsI	GAAGAC	1 cut(s)	1754
Bbv12I	GWGCWC	3 cut(s)	160, 1012, 1630
BbvCI	CCTCAGC	1 cut(s)	2025
BbvI	GCAGC	8 cut(s)	569, 737, 1076, 1136, 1139, 1387, 2554, 2800
BccI	CCATC	3 cut(s)	1728, 2159, 2606
BceAI	ACGGC	2 cut(s)	1423, 2107
BcgI	CGANNNNNNTGC	2 cut(s)	765, 799
BciT130I	CCWGG	6 cut(s)	459, 802, 1224, 1260, 1338, 1380
BciVI	GTATCC	1 cut(s)	1665
BclI	TGATCA	3 cut(s)	496, 1867, 2275
BcnI	CCSGG	1 cut(s)	1575
BcoDI	GTCTC	6 cut(s)	96, 459, 792, 835, 861, 2434
BcuI	ACTAGT	2 cut(s)	248, 1529
BfaI	CTAG	5 cut(s)	240, 249, 699, 1488, 1530
BfmI	CTRYAG	5 cut(s)	612, 1909, 2081, 2568, 2821
BfuI	GTATCC	1 cut(s)	1665
BlnI	CCTAGG	1 cut(s)	698
BlpI	GCTNAGC	1 cut(s)	2510
Bme1390I	CCNGG	7 cut(s)	459, 802, 1224, 1260, 1338, 1380, 1575
Bme18I	GGWCC	4 cut(s)	416, 1598, 2410, 2644
BmgT120I	GGNCC	7 cut(s)	416, 702, 1571, 1598, 2410, 2603, 2644
BmiI	GGNNCC	7 cut(s)	28, 417, 418, 648, 1745, 2020, 2818
BmrFI	CCNGG	7 cut(s)	459, 802, 1224, 1260, 1338, 1380, 1575
BmrI	ACTGGG	1 cut(s)	31
BmsI	GCATC	8 cut(s)	62, 811, 1060, 1493, 1638, 2241, 2308, 2537
BmuI	ACTGGG	1 cut(s)	31
BpiI	GAAGAC	1 cut(s)	1754
BplI	GAGNNNNNCTC	2 cut(s)	1932, 1964
BpmI	CTGGAG	5 cut(s)	441, 567, 1070, 1242, 2354
Bpu10I	CCTNAGC	2 cut(s)	2025, 2406
Bpu1102I	GCTNAGC	1 cut(s)	2510
BpuEI	CTTGAG	3 cut(s)	619, 987, 1811
BpuMI	CCSGG	1 cut(s)	1575
BsaBI	GATNNNNATC	1 cut(s)	1038
BsaHI	GRCGYC	1 cut(s)	376
BsaI	GGTCTC	1 cut(s)	96
BsaJI	CCNNGG	8 cut(s)	698, 1222, 1259, 1337, 1379, 1559, 1574, 1747
BsaXI	ACNNNNNCTCC	2 cut(s)	796, 826
Bsc4I	CCNNNNNNNGG	5 cut(s)	169, 413, 1241, 1637, 2612
Bse118I	RCCGGY	2 cut(s)	40, 1078
Bse1I	ACTGG	8 cut(s)	16, 26, 232, 765, 1468, 1723, 2362, 2413
Bse21I	CCTNAGG	1 cut(s)	651
Bse3DI	GCAATG	3 cut(s)	443, 646, 2363
Bse8I	GATNNNNATC	1 cut(s)	1038
BseBI	CCWGG	6 cut(s)	459, 802, 1224, 1260, 1338, 1380
BseDI	CCNNGG	8 cut(s)	698, 1222, 1259, 1337, 1379, 1559, 1574, 1747
BseJI	GATNNNNATC	1 cut(s)	1038
BseLI	CCNNNNNNNGG	5 cut(s)	169, 413, 1241, 1637, 2612
BseMI	GCAATG	3 cut(s)	443, 646, 2363
BseMII	CTCAG	8 cut(s)	89, 264, 642, 677, 1004, 2039, 2420, 2501
BseNI	ACTGG	8 cut(s)	16, 26, 232, 765, 1468, 1723, 2362, 2413
BseRI	GAGGAG	5 cut(s)	95, 290, 668, 990, 1808
BseSI	GKGCMC	3 cut(s)	160, 1630, 1746
BseXI	GCAGC	8 cut(s)	569, 737, 1076, 1136, 1139, 1387, 2554, 2800
BseYI	CCCAGC	3 cut(s)	207, 911, 2433
BsgI	GTGCAG	3 cut(s)	744, 1000, 2687
BshFI	GGCC	8 cut(s)	188, 703, 907, 1467, 1573, 1815, 2061, 2604
BshNI	GGYRCC	2 cut(s)	646, 1743
BsiHKAI	GWGCWC	3 cut(s)	160, 1012, 1630
BsiSI	CCGG	3 cut(s)	41, 1079, 1574
BslFI	GGGAC	1 cut(s)	429
BslI	CCNNNNNNNGG	5 cut(s)	169, 413, 1241, 1637, 2612
BsmAI	GTCTC	6 cut(s)	96, 459, 792, 835, 861, 2434
BsmBI	CGTCTC	1 cut(s)	792
BsmFI	GGGAC	1 cut(s)	429
BsnI	GGCC	8 cut(s)	188, 703, 907, 1467, 1573, 1815, 2061, 2604
Bso31I	GGTCTC	1 cut(s)	96
Bsp1286I	GDGCHC	6 cut(s)	160, 179, 1012, 1630, 1746, 2731
Bsp1407I	TGTACA	4 cut(s)	2152, 2342, 2503, 2687
Bsp1720I	GCTNAGC	1 cut(s)	2510
BspACI	CCGC	3 cut(s)	908, 1067, 1816
BspANI	GGCC	8 cut(s)	188, 703, 907, 1467, 1573, 1815, 2061, 2604
BspCNI	CTCAG	8 cut(s)	90, 265, 643, 676, 1003, 2038, 2419, 2502
BspHI	TCATGA	2 cut(s)	1765, 2461
BspLI	GGNNCC	7 cut(s)	28, 417, 418, 648, 1745, 2020, 2818
BspMAI	CTGCAG	3 cut(s)	2085, 2572, 2825
BspPI	GGATC	6 cut(s)	287, 1041, 1403, 2046, 2811, 2824
BspT107I	GGYRCC	2 cut(s)	646, 1743
BspTNI	GGTCTC	1 cut(s)	96
BsrDI	GCAATG	3 cut(s)	443, 646, 2363
BsrFI	RCCGGY	2 cut(s)	40, 1078
BsrGI	TGTACA	4 cut(s)	2152, 2342, 2503, 2687
BsrI	ACTGG	8 cut(s)	16, 26, 232, 765, 1468, 1723, 2362, 2413
BssAI	RCCGGY	2 cut(s)	40, 1078
BssECI	CCNNGG	8 cut(s)	698, 1222, 1259, 1337, 1379, 1559, 1574, 1747
BssNI	GRCGYC	1 cut(s)	376
BssSI	CACGAG	2 cut(s)	476, 1135
BssT1I	CCWWGG	1 cut(s)	698
Bst2BI	CACGAG	2 cut(s)	476, 1135
Bst2UI	CCWGG	6 cut(s)	459, 802, 1224, 1260, 1338, 1380
Bst4CI	ACNGT	8 cut(s)	317, 431, 736, 903, 1291, 1910, 2583, 2696
Bst6I	CTCTTC	2 cut(s)	477, 1914
BstACI	GRCGYC	1 cut(s)	376
BstAPI	GCANNNNNTGC	2 cut(s)	50, 1070
BstAUI	TGTACA	4 cut(s)	2152, 2342, 2503, 2687
BstC8I	GCNNGC	5 cut(s)	42, 385, 1612, 2397, 2516
BstMAI	GTCTC	6 cut(s)	96, 459, 792, 835, 861, 2434
BstMWI	GCNNNNNNNGC	7 cut(s)	50, 305, 1070, 1124, 1481, 1815, 2515
BstNI	CCWGG	6 cut(s)	459, 802, 1224, 1260, 1338, 1380
BstNSI	RCATGY	4 cut(s)	877, 1958, 2399, 2441
BstSCI	CCNGG	7 cut(s)	457, 800, 1222, 1258, 1336, 1378, 1573
BstSFI	CTRYAG	5 cut(s)	612, 1909, 2081, 2568, 2821
BstSLI	GKGCMC	3 cut(s)	160, 1630, 1746
BstV1I	GCAGC	8 cut(s)	569, 737, 1076, 1136, 1139, 1387, 2554, 2800
BstV2I	GAAGAC	1 cut(s)	1754
BstX2I	RGATCY	3 cut(s)	292, 2038, 2816
BstXI	CCANNNNNNTGG	1 cut(s)	1534
BstYI	RGATCY	3 cut(s)	292, 2038, 2816
Bsu36I	CCTNAGG	1 cut(s)	651
BsuI	GTATCC	1 cut(s)	1665
BsuRI	GGCC	8 cut(s)	188, 703, 907, 1467, 1573, 1815, 2061, 2604
BtsI	GCAGTG	1 cut(s)	1012
BtsIMutI	CAGTG	4 cut(s)	436, 741, 908, 1012
Cac8I	GCNNGC	5 cut(s)	42, 385, 1612, 2397, 2516
CaiI	CAGNNNCTG	2 cut(s)	11, 1265
CciI	TCATGA	2 cut(s)	1765, 2461
Cfr10I	RCCGGY	2 cut(s)	40, 1078
Cfr13I	GGNCC	7 cut(s)	416, 702, 1571, 1598, 2410, 2603, 2644
CseI	GACGC	2 cut(s)	1503, 2573
Csp6I	GTAC	9 cut(s)	647, 807, 899, 1082, 2089, 2153, 2343, 2504, 2688
CspCI	CAANNNNNGTGG	4 cut(s)	130, 165, 884, 919
CviQI	GTAC	9 cut(s)	647, 807, 899, 1082, 2089, 2153, 2343, 2504, 2688
DraI	TTTAAA	1 cut(s)	1215
DraIII	CACNNNGTG	1 cut(s)	1607
EaeI	YGGCCR	3 cut(s)	186, 905, 1465
Eam1104I	CTCTTC	2 cut(s)	477, 1914
EarI	CTCTTC	2 cut(s)	477, 1914
Ecl136II	GAGCTC	1 cut(s)	1010
Eco130I	CCWWGG	1 cut(s)	698
Eco24I	GRGCYC	3 cut(s)	179, 1012, 2731
Eco31I	GGTCTC	1 cut(s)	96
Eco32I	GATATC	3 cut(s)	204, 1250, 1837
Eco47I	GGWCC	4 cut(s)	416, 1598, 2410, 2644
Eco53kI	GAGCTC	1 cut(s)	1010
Eco81I	CCTNAGG	1 cut(s)	651
EcoICRI	GAGCTC	1 cut(s)	1010
EcoO109I	RGGNCCY	1 cut(s)	416
EcoRII	CCWGG	6 cut(s)	457, 800, 1222, 1258, 1336, 1378
EcoRV	GATATC	3 cut(s)	204, 1250, 1837
EcoT14I	CCWWGG	1 cut(s)	698
EcoT22I	ATGCAT	1 cut(s)	2301
EcoT38I	GRGCYC	3 cut(s)	179, 1012, 2731
ErhI	CCWWGG	1 cut(s)	698
Esp3I	CGTCTC	1 cut(s)	792
FalI	AAGNNNNNCTT	4 cut(s)	331, 363, 2133, 2165
FaqI	GGGAC	1 cut(s)	429
FauNDI	CATATG	4 cut(s)	450, 1237, 1369, 1453
FbaI	TGATCA	3 cut(s)	496, 1867, 2275
FblI	GTMKAC	1 cut(s)	602
FriOI	GRGCYC	3 cut(s)	179, 1012, 2731
FspBI	CTAG	5 cut(s)	240, 249, 699, 1488, 1530
GsaI	CCCAGC	3 cut(s)	211, 915, 2437
GsuI	CTGGAG	5 cut(s)	441, 567, 1070, 1242, 2354
HaeIII	GGCC	8 cut(s)	188, 703, 907, 1467, 1573, 1815, 2061, 2604
HapII	CCGG	3 cut(s)	41, 1079, 1574
HgaI	GACGC	2 cut(s)	1503, 2573
Hin1I	GRCGYC	1 cut(s)	376
HincII	GTYRAC	1 cut(s)	2699
HindII	GTYRAC	1 cut(s)	2699
HindIII	AAGCTT	2 cut(s)	232, 1300
HpaII	CCGG	3 cut(s)	41, 1079, 1574
HphI	GGTGA	5 cut(s)	600, 1034, 1326, 1656, 2573
Hpy166II	GTNNAC	5 cut(s)	158, 603, 660, 1628, 2699
Hpy8I	GTNNAC	5 cut(s)	158, 603, 660, 1628, 2699
Hpy99I	CGWCG	2 cut(s)	378, 787
HpyAV	CCTTC	5 cut(s)	357, 1918, 2029, 2635, 2665
HpyCH4III	ACNGT	8 cut(s)	317, 431, 736, 903, 1291, 1910, 2583, 2696
HpyCH4IV	ACGT	2 cut(s)	376, 721
HpyF10VI	GCNNNNNNNGC	7 cut(s)	50, 305, 1070, 1124, 1481, 1815, 2515
HpySE526I	ACGT	2 cut(s)	376, 721
Hsp92I	GRCGYC	1 cut(s)	376
KflI	GGGWCCC	1 cut(s)	416
KpnI	GGTACC	1 cut(s)	650
KroI	GCCGGC	1 cut(s)	40
KroNI	GCCGGC	1 cut(s)	42
Ksp22I	TGATCA	3 cut(s)	496, 1867, 2275
LmnI	GCTCC	4 cut(s)	277, 1007, 1480, 2335
Lsp1109I	GCAGC	8 cut(s)	569, 737, 1076, 1136, 1139, 1387, 2554, 2800
LweI	GCATC	8 cut(s)	62, 811, 1060, 1493, 1638, 2241, 2308, 2537
MaeI	CTAG	5 cut(s)	240, 249, 699, 1488, 1530
MaeII	ACGT	2 cut(s)	376, 721
MaeIII	GTNAC	3 cut(s)	1985, 2189, 2561
MboII	GAAGA	9 cut(s)	48, 494, 599, 728, 1722, 1754, 1931, 2758, 2813
MfeI	CAATTG	1 cut(s)	563
MflI	RGATCY	3 cut(s)	292, 2038, 2816
MhlI	GDGCHC	6 cut(s)	160, 179, 1012, 1630, 1746, 2731
MlsI	TGGCCA	2 cut(s)	188, 1467
MluNI	TGGCCA	2 cut(s)	188, 1467
MlyI	GAGTC	7 cut(s)	209, 278, 494, 1457, 1768, 1888, 2381
MmeI	TCCRAC	3 cut(s)	1576, 1881, 2142
Mox20I	TGGCCA	2 cut(s)	188, 1467
Mph1103I	ATGCAT	1 cut(s)	2301
MroNI	GCCGGC	1 cut(s)	40
MroXI	GAANNNNTTC	2 cut(s)	926, 1330
MscI	TGGCCA	2 cut(s)	188, 1467
MslI	CAYNNNNRTG	2 cut(s)	2304, 2736
Msp20I	TGGCCA	2 cut(s)	188, 1467
MspA1I	CMGCKG	4 cut(s)	728, 1067, 2509, 2826
MspI	CCGG	3 cut(s)	41, 1079, 1574
MspR9I	CCNGG	7 cut(s)	459, 802, 1224, 1260, 1338, 1380, 1575
MunI	CAATTG	1 cut(s)	563
MvaI	CCWGG	6 cut(s)	459, 802, 1224, 1260, 1338, 1380
MwoI	GCNNNNNNNGC	7 cut(s)	50, 305, 1070, 1124, 1481, 1815, 2515
NaeI	GCCGGC	1 cut(s)	42
NciI	CCSGG	1 cut(s)	1575
NdeI	CATATG	4 cut(s)	450, 1237, 1369, 1453
NgoMIV	GCCGGC	1 cut(s)	40
NlaIV	GGNNCC	7 cut(s)	28, 417, 418, 648, 1745, 2020, 2818
NmeAIII	GCCGAG	1 cut(s)	2339
NmuCI	GTSAC	2 cut(s)	2189, 2561
NsiI	ATGCAT	1 cut(s)	2301
NspI	RCATGY	4 cut(s)	877, 1958, 2399, 2441
PaeI	GCATGC	1 cut(s)	2399
PagI	TCATGA	2 cut(s)	1765, 2461
PdiI	GCCGGC	1 cut(s)	42
PdmI	GAANNNNTTC	2 cut(s)	926, 1330
PfeI	GAWTC	6 cut(s)	334, 370, 590, 842, 892, 1996
PflMI	CCANNNNNTGG	2 cut(s)	1241, 1637
PfoI	TCCNGGA	1 cut(s)	457
PleI	GAGTC	7 cut(s)	209, 277, 493, 1456, 1767, 1887, 2381
PpsI	GAGTC	7 cut(s)	209, 277, 493, 1456, 1767, 1887, 2381
PpuMI	RGGWCCY	1 cut(s)	416
PshBI	ATTAAT	3 cut(s)	192, 1967, 2715
PsiI	TTATAA	2 cut(s)	1782, 2258
Psp124BI	GAGCTC	1 cut(s)	1012
Psp5II	RGGWCCY	1 cut(s)	416
Psp6I	CCWGG	6 cut(s)	457, 800, 1222, 1258, 1336, 1378
PspFI	CCCAGC	3 cut(s)	207, 911, 2433
PspGI	CCWGG	6 cut(s)	457, 800, 1222, 1258, 1336, 1378
PspN4I	GGNNCC	7 cut(s)	28, 417, 418, 648, 1745, 2020, 2818
PspPI	GGNCC	7 cut(s)	416, 702, 1571, 1598, 2410, 2603, 2644
PspPPI	RGGWCCY	1 cut(s)	416
PstI	CTGCAG	3 cut(s)	2085, 2572, 2825
PstNI	CAGNNNCTG	2 cut(s)	11, 1265
PsuI	RGATCY	3 cut(s)	292, 2038, 2816
PvuII	CAGCTG	3 cut(s)	728, 2509, 2826
RsaI	GTAC	9 cut(s)	648, 808, 900, 1083, 2090, 2154, 2344, 2505, 2689
RsaNI	GTAC	9 cut(s)	647, 807, 899, 1082, 2089, 2153, 2343, 2504, 2688
RseI	CAYNNNNRTG	2 cut(s)	2304, 2736
SacI	GAGCTC	1 cut(s)	1012
Sau96I	GGNCC	7 cut(s)	416, 702, 1571, 1598, 2410, 2603, 2644
SchI	GAGTC	7 cut(s)	209, 278, 494, 1457, 1768, 1888, 2381
ScrFI	CCNGG	7 cut(s)	459, 802, 1224, 1260, 1338, 1380, 1575
SduI	GDGCHC	6 cut(s)	160, 179, 1012, 1630, 1746, 2731
SfaNI	GCATC	8 cut(s)	62, 811, 1060, 1493, 1638, 2241, 2308, 2537
SfcI	CTRYAG	5 cut(s)	612, 1909, 2081, 2568, 2821
SinI	GGWCC	4 cut(s)	416, 1598, 2410, 2644
SmiMI	CAYNNNNRTG	2 cut(s)	2304, 2736
SmlI	CTYRAG	3 cut(s)	634, 1002, 1790
SmoI	CTYRAG	3 cut(s)	634, 1002, 1790
SpeI	ACTAGT	2 cut(s)	248, 1529
SphI	GCATGC	1 cut(s)	2399
SsiI	CCGC	3 cut(s)	908, 1067, 1816
SspMI	CTAG	5 cut(s)	240, 249, 699, 1488, 1530
SstI	GAGCTC	1 cut(s)	1012
StyD4I	CCNGG	7 cut(s)	457, 800, 1222, 1258, 1336, 1378, 1573
StyI	CCWWGG	1 cut(s)	698
TaaI	ACNGT	8 cut(s)	317, 431, 736, 903, 1291, 1910, 2583, 2696
TaiI	ACGT	2 cut(s)	379, 724
TaqI	TCGA	6 cut(s)	145, 373, 869, 1312, 1882, 2779
TatI	WGTACW	6 cut(s)	806, 898, 2152, 2342, 2503, 2687
TauI	GCSGC	2 cut(s)	910, 1818
TfiI	GAWTC	6 cut(s)	334, 370, 590, 842, 892, 1996
TscAI	CASTG	4 cut(s)	436, 741, 908, 1019
TseFI	GTSAC	2 cut(s)	2189, 2561
TseI	GCWGC	9 cut(s)	557, 725, 1064, 1124, 1127, 1375, 2567, 2788, 2823
Tsp45I	GTSAC	2 cut(s)	2189, 2561
TspGWI	ACGGA	1 cut(s)	1140
TspRI	CASTG	4 cut(s)	436, 741, 908, 1019
Van91I	CCANNNNNTGG	2 cut(s)	1241, 1637
VneI	GTGCAC	2 cut(s)	156, 1626
VpaK11BI	GGWCC	4 cut(s)	416, 1598, 2410, 2644
VspI	ATTAAT	3 cut(s)	192, 1967, 2715
XapI	RAATTY	7 cut(s)	1058, 1162, 1579, 1776, 1856, 2064, 2522
XbaI	TCTAGA	1 cut(s)	1487
XceI	RCATGY	4 cut(s)	877, 1958, 2399, 2441
XcmI	CCANNNNNNNNNTGG	1 cut(s)	215
XmaJI	CCTAGG	1 cut(s)	698
XmiI	GTMKAC	1 cut(s)	602
XmnI	GAANNNNTTC	2 cut(s)	926, 1330
XspI	CTAG	5 cut(s)	240, 249, 699, 1488, 1530
ZraI	GACGTC	1 cut(s)	377
Zsp2I	ATGCAT	1 cut(s)	2301

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.