Basic Information

Type: gene

Biological Identity

Species

rosa_multiflora

Chromosome

Rmu_sc0004626.1

Orthogroup ID

OG0010512

Physical Location & Seq

Genomic Coordinates Forward (+)

2254 .. 5812

Gene Structure 3559 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1650 bp

atgggttcgagcactagccgactgggttcgcgcccatctcggccccgacccaaccgcatcgccaatcgttccctactctcctctctcgtctgcggaggctcttcctcacgtgccgctcacgagatggaagatcatccagctgagtgtcttgtgagatacgcagagaattgtgatcggggtaaaaatccagttcagaactccacagacgtatcctcgttgatctccagtgcaggggcagggtccaccttccctaatactgaaattggatcctcatctcagagcgttgttccagccggtcagggtacttattttgaagaaggtttaagaagtcttgacacgagtaaccaggggaagtgcttgtccagaagtaaggagctcatttctcatccggtaagtgctggttgtaatcatgatgaatctagtagggataatagtactacagctagtacttcgtttaaagaacaacaatcttcccacccaatctctgtgaatagttcggctaacatggatgcagtcaatggctccgcagacaagaatgtatctcaggcatgtcctgtggttatgcgtccaagtagttcttctccacaagggattgggaattcccattctgatgacatgtccgttgagaatcatgtagctgaagtaatggatgttcataatactaatgctgatactgattctgttcatgtttctgatataccattaccttttccctcacgcggaaatgattcctttccctcacgcggaaatgattcccttcgggaggtaccttctggcttaggattccttgtgtccaacagggaacaaagccgggaagatggaagtgtagttcatgttgatgtcatgagcatttcttcgaactttttgtccagtagtaatgctgatacaagtaaccgtgagtccagaaggaatggtaggcgtctgttttgggatgcattttcaagacgcagttccagaaggtttattgattctccaaccatacttttctctactgatgagactgaggatctaggatttcatgacagatggctacttggtttgagtggtgattttcttgatgatcggagtggagttgattctggacaccgggtcaatagaactcccaacttgagtgaacggcgccggcactctagatctgaggtctgggaacggcttcgtggtggccttgatgataatgctcaccgcaatactacttgtcctacaggaattcacccgggtggcatgtgctcctgtggttcattcttgatgacagaagagtctagtgctcgggcaagtatatcgagaattgtcatgcttgctgaagctctattcgaggttttggatgaaattcatcgccaacctgtgtcactttcactgtctatggtctcagtccctgccccagcatctgtcgttgattcgtttcctcttaagagtcacaccaaggttgacaaattgaagcatggggatgatgctgaacagtgccacatctgccttgctgagtatgaggaaggggacaaaattcgagttctgccctgccaccatgagtatcacatgtcatgtgtggataagtggcttaaagagatacatggagtttgtccactttgtcgaggagatgttcgtgaaggtttaaacgactgttccgtatctaactcagggatatcttctatttga

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000578 GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006464 GO:0006508 GO:0006511 GO:0006807 GO:0007275 GO:0007389 GO:0008134 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009790 GO:0009798 GO:0009880 GO:0009987 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0019941 GO:0030163 GO:0032446 GO:0032501 GO:0032502 GO:0036211 GO:0042802 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044424 GO:0044464 GO:0048856 GO:0051603 GO:0070647 GO:0071704 GO:0140096 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

zf-RING_11 zf-RING_2

Protein Analysis

549

Amino Acids

59.63

Weight (kDa)

5.79

Isoelectric Point (pI)

63.19

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-rbx1	PF12678	485 - 529	2.1e-07	RING-H2 zinc finger domain
zf-RING_2	PF13639	486 - 529	3.1e-12	Ring finger domain
zf-RING_11	PF17123	486 - 514	3.3e-08	RING-like zinc finger
zf-C3HC4_2	PF13923	486 - 528	3.8e-06	Zinc finger, C3HC4 type (RING finger)
zf-C3HC4	PF00097	487 - 528	9.1e-08	Zinc finger, C3HC4 type (RING finger)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010512)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_1g01770
malus_domestica	MD02G1013400.v1.1 MD15G1160200.v1.1
prunus_persica	Prupe.7G255200_v2.0.a1 Prupe.7G255200_v2.0.a1 Prupe.7G255200_v2.0.a1
pyrus_communis	pycom02g01250 pycom15g14410
rosa_chinensis	RchiOBHm_Chr2g0086731
rosa_laevigata	RLG00000015786
rosa_multiflora	Rmu_sc0000335.1_g000009 Rmu_sc0004626.1_g000001 Rmu_sc0030160.1_g000001 Rmu_sc0035211.1_g000001
rosa_roxburghii	Rroxscaffold_2G00154240
rosa_rugosa	Rorug01G0469200
rosa_samantha	Rh2AG021300 Rh2BG021200 Rh2CG022100 Rh2DG021900
rosa_wichuraiana	Rw2G001730

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1255
Acc65I	GGTACC	1 cut(s)	766
AccB1I	GGYRCC	2 cut(s)	766, 1119
AccBSI	CCGCTC	1 cut(s)	116
AccII	CGCG	3 cut(s)	31, 720, 744
AciI	CCGC	7 cut(s)	55, 93, 114, 525, 720, 744, 1183
AclWI	GGATC	3 cut(s)	261, 274, 1014
AcsI	RAATTY	4 cut(s)	598, 1206, 1326, 1497
AcuI	CTGAAG	2 cut(s)	660, 1320
AcvI	CACGTG	1 cut(s)	110
AcyI	GRCGYC	2 cut(s)	919, 1120
AfaI	GTAC	4 cut(s)	304, 436, 448, 768
AfiI	CCNNNNNNNGG	4 cut(s)	231, 719, 743, 763
AflII	CTTAAG	1 cut(s)	1406
AflIII	ACRYGT	2 cut(s)	615, 1530
AgsI	TTSAA	3 cut(s)	314, 942, 1435
AhdI	GACNNNNNGTC	1 cut(s)	1088
AjnI	CCWGG	1 cut(s)	345
AjuI	GAANNNNNNNTTGG	2 cut(s)	562, 594
AleI	CACNNNNGTG	1 cut(s)	1215
AloI	GAACNNNNNNTCC	4 cut(s)	813, 845, 851, 883
AluBI	AGCT	5 cut(s)	140, 376, 443, 638, 1304
AluI	AGCT	5 cut(s)	140, 376, 443, 638, 1304
Alw21I	GWGCWC	4 cut(s)	14, 378, 1229, 1267
Alw26I	GTCTC	2 cut(s)	992, 1369
AlwI	GGATC	3 cut(s)	261, 274, 1014
AlwNI	CAGNNNCTG	2 cut(s)	1373, 1385
Ama87I	CYCGRG	2 cut(s)	1213, 1266
AoxI	GGCC	2 cut(s)	41, 1162
ApoI	RAATTY	4 cut(s)	598, 1206, 1326, 1497
ArsI	GACNNNNNNTTYG	2 cut(s)	851, 883
Asp700I	GAANNNNTTC	3 cut(s)	727, 751, 1152
Asp718I	GGTACC	1 cut(s)	766
AspLEI	GCGC	2 cut(s)	33, 1122
AspS9I	GGNCC	2 cut(s)	42, 240
AsuC2I	CCSGG	4 cut(s)	812, 1088, 1214, 1215
AsuHPI	GGTGA	3 cut(s)	1058, 1172, 1202
AsuII	TTCGAA	1 cut(s)	857
AvaI	CYCGRG	2 cut(s)	1213, 1266
AvaII	GGWCC	1 cut(s)	240
BaeI	ACNNNNGTAYC	2 cut(s)	192, 225
BamHI	GGATCC	1 cut(s)	266
BanI	GGYRCC	2 cut(s)	766, 1119
BanII	GRGCYC	1 cut(s)	378
BauI	CACGAG	2 cut(s)	119, 337
BbrPI	CACGTG	1 cut(s)	110
Bbv12I	GWGCWC	4 cut(s)	14, 378, 1229, 1267
BccI	CCATC	4 cut(s)	43, 118, 812, 1020
BceAI	ACGGC	2 cut(s)	1133, 1166
BcgI	CGANNNNNNTGC	2 cut(s)	1260, 1294
BciT130I	CCWGG	1 cut(s)	347
BciVI	GTATCC	1 cut(s)	220
BcnI	CCSGG	4 cut(s)	812, 1088, 1214, 1215
BcoDI	GTCTC	2 cut(s)	992, 1369
BfaI	CTAG	6 cut(s)	15, 420, 444, 1010, 1131, 1260
BfmI	CTRYAG	2 cut(s)	438, 1200
BfoI	RGCGCY	1 cut(s)	1123
BfrI	CTTAAG	1 cut(s)	1406
BfuI	GTATCC	1 cut(s)	220
BglII	AGATCT	1 cut(s)	1133
BisI	GCNGC	1 cut(s)	114
BlsI	GCNGC	1 cut(s)	115
BmcAI	AGTACT	2 cut(s)	436, 448
Bme1390I	CCNGG	5 cut(s)	347, 812, 1088, 1214, 1215
Bme18I	GGWCC	1 cut(s)	240
BmeRI	GACNNNNNGTC	1 cut(s)	1088
BmeT110I	CYCGRG	2 cut(s)	1213, 1266
BmgT120I	GGNCC	2 cut(s)	42, 240
BmiI	GGNNCC	6 cut(s)	44, 241, 268, 523, 768, 1121
BmrFI	CCNGG	5 cut(s)	347, 812, 1088, 1214, 1215
BmrI	ACTGGG	1 cut(s)	32
BmsI	GCATC	5 cut(s)	66, 499, 922, 1391, 1438
BmuI	ACTGGG	1 cut(s)	32
BpmI	CTGGAG	1 cut(s)	208
Bpu10I	CCTNAGC	1 cut(s)	778
Bpu14I	TTCGAA	1 cut(s)	857
BpuEI	CTTGAG	1 cut(s)	1129
BpuMI	CCSGG	4 cut(s)	812, 1088, 1214, 1215
BsaAI	YACGTR	1 cut(s)	110
BsaBI	GATNNNNATC	1 cut(s)	271
BsaHI	GRCGYC	2 cut(s)	919, 1120
BsaI	GGTCTC	1 cut(s)	1369
BsaJI	CCNNGG	3 cut(s)	346, 1213, 1419
BsaWI	WCCGGW	1 cut(s)	388
Bsc4I	CCNNNNNNNGG	4 cut(s)	231, 719, 743, 763
Bse118I	RCCGGY	2 cut(s)	293, 1122
Bse1I	ACTGG	4 cut(s)	27, 188, 225, 870
Bse8I	GATNNNNATC	1 cut(s)	271
BseBI	CCWGG	1 cut(s)	347
BseDI	CCNNGG	3 cut(s)	346, 1213, 1419
BseGI	GGATG	7 cut(s)	133, 385, 514, 655, 937, 1327, 1450
BseJI	GATNNNNATC	1 cut(s)	271
BseLI	CCNNNNNNNGG	4 cut(s)	231, 719, 743, 763
BseMII	CTCAG	8 cut(s)	132, 290, 557, 993, 1128, 1380, 1467, 1644
BseNI	ACTGG	4 cut(s)	27, 188, 225, 870
BseRI	GAGGAG	2 cut(s)	70, 1602
BseYI	CCCAGC	1 cut(s)	1378
BsgI	GTGCAG	1 cut(s)	249
Bsh1236I	CGCG	3 cut(s)	31, 720, 744
BshFI	GGCC	2 cut(s)	43, 1164
BshNI	GGYRCC	2 cut(s)	766, 1119
BsiHKAI	GWGCWC	4 cut(s)	14, 378, 1229, 1267
BsiHKCI	CYCGRG	2 cut(s)	1213, 1266
BsiSI	CCGG	6 cut(s)	294, 389, 811, 1087, 1123, 1214
BslFI	GGGAC	2 cut(s)	1355, 1505
BslI	CCNNNNNNNGG	4 cut(s)	231, 719, 743, 763
BsmAI	GTCTC	2 cut(s)	992, 1369
BsmFI	GGGAC	2 cut(s)	1355, 1505
BsnI	GGCC	2 cut(s)	43, 1164
Bso31I	GGTCTC	1 cut(s)	1369
BsoBI	CYCGRG	2 cut(s)	1213, 1266
Bsp119I	TTCGAA	1 cut(s)	857
Bsp1286I	GDGCHC	4 cut(s)	14, 378, 1229, 1267
Bsp143I	GATC	7 cut(s)	130, 172, 219, 266, 1006, 1060, 1133
BspACI	CCGC	7 cut(s)	55, 93, 114, 525, 720, 744, 1183
BspANI	GGCC	2 cut(s)	43, 1164
BspCNI	CTCAG	8 cut(s)	133, 289, 556, 994, 1129, 1379, 1468, 1643
BspFNI	CGCG	3 cut(s)	31, 720, 744
BspHI	TCATGA	3 cut(s)	409, 843, 1018
BspLI	GGNNCC	6 cut(s)	44, 241, 268, 523, 768, 1121
BspPI	GGATC	3 cut(s)	261, 274, 1014
BspQI	GCTCTTC	1 cut(s)	106
BspT104I	TTCGAA	1 cut(s)	857
BspT107I	GGYRCC	2 cut(s)	766, 1119
BspTI	CTTAAG	1 cut(s)	1406
BspTNI	GGTCTC	1 cut(s)	1369
BsrBI	CCGCTC	1 cut(s)	116
BsrFI	RCCGGY	2 cut(s)	293, 1122
BsrI	ACTGG	4 cut(s)	27, 188, 225, 870
BssAI	RCCGGY	2 cut(s)	293, 1122
BssECI	CCNNGG	3 cut(s)	346, 1213, 1419
BssMI	GATC	7 cut(s)	130, 172, 219, 266, 1006, 1060, 1133
BssNI	GRCGYC	2 cut(s)	919, 1120
BssSI	CACGAG	2 cut(s)	119, 337
BssT1I	CCWWGG	1 cut(s)	1419
Bst2BI	CACGAG	2 cut(s)	119, 337
Bst2UI	CCWGG	1 cut(s)	347
Bst4CI	ACNGT	4 cut(s)	896, 1356, 1458, 1616
Bst6I	CTCTTC	2 cut(s)	106, 1248
BstACI	GRCGYC	2 cut(s)	919, 1120
BstAFI	CTTAAG	1 cut(s)	1406
BstBAI	YACGTR	1 cut(s)	110
BstBI	TTCGAA	1 cut(s)	857
BstC8I	GCNNGC	2 cut(s)	1124, 1296
BstDEI	CTNAG	9 cut(s)	141, 276, 543, 778, 1002, 1137, 1366, 1476, 1630
BstENI	CCTNNNNNAGG	1 cut(s)	761
BstF5I	GGATG	7 cut(s)	133, 385, 514, 655, 937, 1327, 1450
BstFNI	CGCG	3 cut(s)	31, 720, 744
BstH2I	RGCGCY	1 cut(s)	1123
BstHHI	GCGC	2 cut(s)	33, 1122
BstKTI	GATC	7 cut(s)	133, 175, 222, 269, 1009, 1063, 1136
BstMAI	GTCTC	2 cut(s)	992, 1369
BstMBI	GATC	7 cut(s)	130, 172, 219, 266, 1006, 1060, 1133
BstMWI	GCNNNNNNNGC	1 cut(s)	1467
BstNI	CCWGG	1 cut(s)	347
BstNSI	RCATGY	4 cut(s)	552, 619, 1225, 1534
BstSCI	CCNGG	5 cut(s)	345, 810, 1086, 1212, 1213
BstSFI	CTRYAG	2 cut(s)	438, 1200
BstUI	CGCG	3 cut(s)	31, 720, 744
BstX2I	RGATCY	3 cut(s)	266, 1006, 1133
BstYI	RGATCY	3 cut(s)	266, 1006, 1133
BsuI	GTATCC	1 cut(s)	220
BsuRI	GGCC	2 cut(s)	43, 1164
BtgZI	GCGATG	2 cut(s)	43, 1316
BtsCI	GGATG	7 cut(s)	133, 385, 514, 655, 937, 1327, 1450
BtsIMutI	CAGTG	3 cut(s)	232, 1352, 1463
Cac8I	GCNNGC	2 cut(s)	1124, 1296
CaiI	CAGNNNCTG	2 cut(s)	1373, 1385
CciI	TCATGA	3 cut(s)	409, 843, 1018
CfoI	GCGC	2 cut(s)	33, 1122
Cfr10I	RCCGGY	2 cut(s)	293, 1122
Cfr13I	GGNCC	2 cut(s)	42, 240
Cfr9I	CCCGGG	1 cut(s)	1213
CseI	GACGC	3 cut(s)	554, 908, 954
Csp6I	GTAC	4 cut(s)	303, 435, 447, 767
CviQI	GTAC	4 cut(s)	303, 435, 447, 767
DdeI	CTNAG	9 cut(s)	141, 276, 543, 778, 1002, 1137, 1366, 1476, 1630
DinI	GGCGCC	1 cut(s)	1121
DpnI	GATC	7 cut(s)	132, 174, 221, 268, 1008, 1062, 1135
DpnII	GATC	7 cut(s)	130, 172, 219, 266, 1006, 1060, 1133
DraI	TTTAAA	2 cut(s)	457, 1608
DrdI	GACNNNNNNGTC	1 cut(s)	1255
DriI	GACNNNNNGTC	1 cut(s)	1088
DseDI	GACNNNNNNGTC	1 cut(s)	1255
Eam1104I	CTCTTC	2 cut(s)	106, 1248
Eam1105I	GACNNNNNGTC	1 cut(s)	1088
EarI	CTCTTC	2 cut(s)	106, 1248
Ecl136II	GAGCTC	1 cut(s)	376
Eco130I	CCWWGG	1 cut(s)	1419
Eco24I	GRGCYC	1 cut(s)	378
Eco31I	GGTCTC	1 cut(s)	1369
Eco32I	GATATC	1 cut(s)	1638
Eco47I	GGWCC	1 cut(s)	240
Eco53kI	GAGCTC	1 cut(s)	376
Eco57I	CTGAAG	2 cut(s)	660, 1320
Eco72I	CACGTG	1 cut(s)	110
Eco88I	CYCGRG	2 cut(s)	1213, 1266
EcoICRI	GAGCTC	1 cut(s)	376
EcoNI	CCTNNNNNAGG	1 cut(s)	761
EcoRI	GAATTC	2 cut(s)	598, 1206
EcoRII	CCWGG	1 cut(s)	345
EcoRV	GATATC	1 cut(s)	1638
EcoT14I	CCWWGG	1 cut(s)	1419
EcoT22I	ATGCAT	1 cut(s)	937
EcoT38I	GRGCYC	1 cut(s)	378
EgeI	GGCGCC	1 cut(s)	1121
EheI	GGCGCC	1 cut(s)	1121
ErhI	CCWWGG	1 cut(s)	1419
FalI	AAGNNNNNCTT	2 cut(s)	562, 594
FaqI	GGGAC	2 cut(s)	1355, 1505
Fnu4HI	GCNGC	1 cut(s)	114
FokI	GGATG	7 cut(s)	120, 372, 521, 662, 944, 1334, 1457
FriOI	GRGCYC	1 cut(s)	378
Fsp4HI	GCNGC	1 cut(s)	114
FspBI	CTAG	6 cut(s)	15, 420, 444, 1010, 1131, 1260
GlaI	GCGC	2 cut(s)	32, 1121
GluI	GCNGC	1 cut(s)	114
GsaI	CCCAGC	1 cut(s)	1382
GsuI	CTGGAG	1 cut(s)	208
HaeII	RGCGCY	1 cut(s)	1123
HaeIII	GGCC	2 cut(s)	43, 1164
HapII	CCGG	6 cut(s)	294, 389, 811, 1087, 1123, 1214
HgaI	GACGC	3 cut(s)	554, 908, 954
HhaI	GCGC	2 cut(s)	33, 1122
Hin1I	GRCGYC	2 cut(s)	919, 1120
Hin6I	GCGC	2 cut(s)	31, 1120
HinP1I	GCGC	2 cut(s)	31, 1120
HincII	GTYRAC	1 cut(s)	1426
HindII	GTYRAC	1 cut(s)	1426
HpaII	CCGG	6 cut(s)	294, 389, 811, 1087, 1123, 1214
HphI	GGTGA	3 cut(s)	1058, 1172, 1202
Hpy166II	GTNNAC	4 cut(s)	243, 1115, 1426, 1577
Hpy188I	TCNGA	6 cut(s)	195, 279, 610, 694, 1065, 1138
Hpy8I	GTNNAC	4 cut(s)	243, 1115, 1426, 1577
HpyAV	CCTTC	8 cut(s)	256, 311, 767, 780, 900, 951, 1481, 1595
HpyCH4III	ACNGT	4 cut(s)	896, 1356, 1458, 1616
HpyCH4IV	ACGT	2 cut(s)	109, 207
HpyCH4V	TGCA	3 cut(s)	230, 512, 935
HpyF10VI	GCNNNNNNNGC	1 cut(s)	1467
HpyF3I	CTNAG	9 cut(s)	141, 276, 543, 778, 1002, 1137, 1366, 1476, 1630
HpySE526I	ACGT	2 cut(s)	109, 207
Hsp92I	GRCGYC	2 cut(s)	919, 1120
HspAI	GCGC	2 cut(s)	31, 1120
KasI	GGCGCC	1 cut(s)	1119
KpnI	GGTACC	1 cut(s)	770
KroI	GCCGGC	1 cut(s)	1122
KroNI	GCCGGC	1 cut(s)	1124
Kzo9I	GATC	7 cut(s)	130, 172, 219, 266, 1006, 1060, 1133
LguI	GCTCTTC	1 cut(s)	106
LmnI	GCTCC	3 cut(s)	373, 527, 1232
LweI	GCATC	5 cut(s)	66, 499, 922, 1391, 1438
MaeI	CTAG	6 cut(s)	15, 420, 444, 1010, 1131, 1260
MaeII	ACGT	2 cut(s)	109, 207
MaeIII	GTNAC	4 cut(s)	341, 890, 1344, 1412
MalI	GATC	7 cut(s)	132, 174, 221, 268, 1008, 1062, 1135
MbiI	CCGCTC	1 cut(s)	116
MboI	GATC	7 cut(s)	130, 172, 219, 266, 1006, 1060, 1133
MboII	GAAGA	9 cut(s)	93, 140, 326, 462, 570, 827, 846, 1265, 1632
MflI	RGATCY	3 cut(s)	266, 1006, 1133
MhlI	GDGCHC	4 cut(s)	14, 378, 1229, 1267
MluCI	AATT	8 cut(s)	166, 261, 598, 1206, 1284, 1326, 1430, 1497
Mly113I	GGCGCC	1 cut(s)	1120
MlyI	GAGTC	3 cut(s)	908, 1265, 1420
MmeI	TCCRAC	2 cut(s)	819, 998
Mph1103I	ATGCAT	1 cut(s)	937
MroNI	GCCGGC	1 cut(s)	1122
MroXI	GAANNNNTTC	3 cut(s)	727, 751, 1152
MseI	TTAA	5 cut(s)	323, 456, 1407, 1554, 1607
MslI	CAYNNNNRTG	4 cut(s)	609, 837, 1215, 1443
MspA1I	CMGCKG	1 cut(s)	140
MspCI	CTTAAG	1 cut(s)	1406
MspI	CCGG	6 cut(s)	294, 389, 811, 1087, 1123, 1214
MspR9I	CCNGG	5 cut(s)	347, 812, 1088, 1214, 1215
MssI	GTTTAAAC	1 cut(s)	1608
MvaI	CCWGG	1 cut(s)	347
MvnI	CGCG	3 cut(s)	31, 720, 744
MwoI	GCNNNNNNNGC	1 cut(s)	1467
NaeI	GCCGGC	1 cut(s)	1124
NarI	GGCGCC	1 cut(s)	1120
NciI	CCSGG	4 cut(s)	812, 1088, 1214, 1215
NdeII	GATC	7 cut(s)	130, 172, 219, 266, 1006, 1060, 1133
NgoMIV	GCCGGC	1 cut(s)	1122
NlaIV	GGNNCC	6 cut(s)	44, 241, 268, 523, 768, 1121
NmeAIII	GCCGAG	1 cut(s)	19
NmuCI	GTSAC	2 cut(s)	1344, 1412
NsiI	ATGCAT	1 cut(s)	937
NspI	RCATGY	4 cut(s)	552, 619, 1225, 1534
NspV	TTCGAA	1 cut(s)	857
OliI	CACNNNNGTG	1 cut(s)	1215
PagI	TCATGA	3 cut(s)	409, 843, 1018
PciI	ACATGT	2 cut(s)	615, 1530
PciSI	GCTCTTC	1 cut(s)	106
PcsI	WCGNNNNNNNCGW	1 cut(s)	1617
PdiI	GCCGGC	1 cut(s)	1124
PdmI	GAANNNNTTC	3 cut(s)	727, 751, 1152
PfeI	GAWTC	9 cut(s)	416, 628, 677, 728, 752, 783, 968, 1076, 1394
PkrI	GCNGC	1 cut(s)	115
PleI	GAGTC	3 cut(s)	907, 1264, 1419
PluTI	GGCGCC	1 cut(s)	1123
PmaCI	CACGTG	1 cut(s)	110
PmeI	GTTTAAAC	1 cut(s)	1608
PmlI	CACGTG	1 cut(s)	110
PpsI	GAGTC	3 cut(s)	907, 1264, 1419
Ppu21I	YACGTR	1 cut(s)	110
PscI	ACATGT	2 cut(s)	615, 1530
Psp124BI	GAGCTC	1 cut(s)	378
Psp6I	CCWGG	1 cut(s)	345
PspCI	CACGTG	1 cut(s)	110
PspFI	CCCAGC	1 cut(s)	1378
PspGI	CCWGG	1 cut(s)	345
PspN4I	GGNNCC	6 cut(s)	44, 241, 268, 523, 768, 1121
PspPI	GGNCC	2 cut(s)	42, 240
PsrI	GAACNNNNNNTAC	2 cut(s)	636, 668
PstNI	CAGNNNCTG	2 cut(s)	1373, 1385
PsuI	RGATCY	3 cut(s)	266, 1006, 1133
PvuII	CAGCTG	1 cut(s)	140
RsaI	GTAC	4 cut(s)	304, 436, 448, 768
RsaNI	GTAC	4 cut(s)	303, 435, 447, 767
RseI	CAYNNNNRTG	4 cut(s)	609, 837, 1215, 1443
SacI	GAGCTC	1 cut(s)	378
SapI	GCTCTTC	1 cut(s)	106
SaqAI	TTAA	5 cut(s)	323, 456, 1407, 1554, 1607
SatI	GCNGC	1 cut(s)	114
Sau3AI	GATC	7 cut(s)	130, 172, 219, 266, 1006, 1060, 1133
Sau96I	GGNCC	2 cut(s)	42, 240
ScaI	AGTACT	2 cut(s)	436, 448
SchI	GAGTC	3 cut(s)	908, 1265, 1420
ScrFI	CCNGG	5 cut(s)	347, 812, 1088, 1214, 1215
SduI	GDGCHC	4 cut(s)	14, 378, 1229, 1267
SfaNI	GCATC	5 cut(s)	66, 499, 922, 1391, 1438
SfcI	CTRYAG	2 cut(s)	438, 1200
SfoI	GGCGCC	1 cut(s)	1121
SfuI	TTCGAA	1 cut(s)	857
SinI	GGWCC	1 cut(s)	240
SmaI	CCCGGG	1 cut(s)	1215
SmiMI	CAYNNNNRTG	4 cut(s)	609, 837, 1215, 1443
SmlI	CTYRAG	2 cut(s)	1108, 1406
SmoI	CTYRAG	2 cut(s)	1108, 1406
Sse9I	AATT	8 cut(s)	166, 261, 598, 1206, 1284, 1326, 1430, 1497
SsiI	CCGC	7 cut(s)	55, 93, 114, 525, 720, 744, 1183
SspDI	GGCGCC	1 cut(s)	1119
SspMI	CTAG	6 cut(s)	15, 420, 444, 1010, 1131, 1260
SstI	GAGCTC	1 cut(s)	378
StyD4I	CCNGG	5 cut(s)	345, 810, 1086, 1212, 1213
StyI	CCWWGG	1 cut(s)	1419
TaaI	ACNGT	4 cut(s)	896, 1356, 1458, 1616
TaiI	ACGT	2 cut(s)	112, 210
TaqI	TCGA	6 cut(s)	8, 857, 1280, 1311, 1501, 1585
TasI	AATT	8 cut(s)	166, 261, 598, 1206, 1284, 1326, 1430, 1497
TatI	WGTACW	2 cut(s)	434, 446
TauI	GCSGC	1 cut(s)	116
TfiI	GAWTC	9 cut(s)	416, 628, 677, 728, 752, 783, 968, 1076, 1394
Tru1I	TTAA	5 cut(s)	323, 456, 1407, 1554, 1607
Tru9I	TTAA	5 cut(s)	323, 456, 1407, 1554, 1607
TscAI	CASTG	3 cut(s)	232, 1359, 1463
TseFI	GTSAC	2 cut(s)	1344, 1412
Tsp45I	GTSAC	2 cut(s)	1344, 1412
TspDTI	ATGAA	8 cut(s)	429, 644, 674, 821, 1007, 1227, 1319, 1338
TspGWI	ACGGA	2 cut(s)	610, 1609
TspMI	CCCGGG	1 cut(s)	1213
TspRI	CASTG	3 cut(s)	232, 1359, 1463
Vha464I	CTTAAG	1 cut(s)	1406
VpaK11BI	GGWCC	1 cut(s)	240
XagI	CCTNNNNNAGG	1 cut(s)	761
XapI	RAATTY	4 cut(s)	598, 1206, 1326, 1497
XbaI	TCTAGA	1 cut(s)	1130
XceI	RCATGY	4 cut(s)	552, 619, 1225, 1534
XmaI	CCCGGG	1 cut(s)	1213
XmnI	GAANNNNTTC	3 cut(s)	727, 751, 1152
XspI	CTAG	6 cut(s)	15, 420, 444, 1010, 1131, 1260
ZrmI	AGTACT	2 cut(s)	436, 448
Zsp2I	ATGCAT	1 cut(s)	937

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.