Rmu_sc0004796.1_g000010

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004796.1
OG0013532
Physical Location & Seq
Forward (+)
69502 .. 70518
1017 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1017 bp
atggtcgacttcccgaaatccctgtcccccaagcgcgtcctcaagctcctccaagccgaaaagaacacccactccgcactcgccctcctcgactccgccactcgccaccccaactacacccactcccccgacgtcttccaccacatcctccgccgcctcatccaccccaacctcgtcgcccacgtcgctcgggtcgtcgaactcatccgcacccagaaatgccactgccccgaggacgtggcactgaccgtcatcaaagcctacgccaaaaactcaatgcctgataaagccctgcacgtcttccagcaaatgcgagagattttcgggtgcgagcccggcattcgatcctacaattctcttctaaacgccttcattgagtccaaccagtgggaccgagccgagcagttctttgcttttttcgaaacggtggggttgtccccaaatctgcagacgtacaacactctgatcaagatttcgtgcaagaagaagcagtttgagaaggccagggggctgctggattggatgtgggagaagggtttggagcctgatgtgatgagttacggaactttgatcaatgcgttggccaaaaatgggaggctgggggatgcattggaggtgttcgacgaaatgcctgagagaggactcagtcctgatgtcatgtgttataatattttgattgatgggttttttcggaaaggcgaatatgtggaggctaaggagctttgggagaggttggtgatggacccggaggcatatccgaatgctgttagttataatgttatgattagtggtttgtgcaaatgcgggaggtttggtgagactttggagatatggaataggatgacgaggaatgaaagaggatgtgatttgtttacttgtagttctttgattcatgggttgtgtaaagcggggaatgtggatgaggcggagagagtttataaggatatggttgagaagggagtagtgccggatgcggttatgtataatgcaatgctcgatggtttctgccctgtgtaa

Protein Analysis

338

Amino Acids

38.52

Weight (kDa)

6.38

Isoelectric Point (pI)

38.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPR_24 PF23276 80 - 209 1.3e-07 Fungal tetratrico peptide repeats
PPR_2 PF13041 83 - 124 1e-06 PPR repeat family
PPR_3 PF13812 110 - 158 1.1e-06 Pentatricopeptide repeat domain
PPR_2 PF13041 112 - 161 4e-09 PPR repeat family
PPR_long PF17177 131 - 233 3.7e-08 Pentacotripeptide-repeat region of PRORP
PPR_1 PF12854 144 - 175 9.2e-06 PPR repeat
PPR_2 PF13041 147 - 196 9.9e-14 PPR repeat family
PPR_3 PF13812 171 - 227 1.1e-11 Pentatricopeptide repeat domain
PPR_1 PF12854 178 - 210 4e-12 PPR repeat
PPR_2 PF13041 182 - 230 3e-16 PPR repeat family
PPR PF01535 186 - 215 1.5e-08 PPR repeat
PPR_1 PF12854 213 - 244 4.6e-09 PPR repeat
PPR_2 PF13041 253 - 302 9.5e-14 PPR repeat family
PPR_1 PF12854 253 - 282 4.1e-08 PPR repeat
PPR PF01535 256 - 284 2.2e-07 PPR repeat
PPR_3 PF13812 281 - 333 2.9e-07 Pentatricopeptide repeat domain
PPR_2 PF13041 289 - 336 8.3e-15 PPR repeat family
PPR_1 PF12854 289 - 317 1.1e-08 PPR repeat
PPR PF01535 291 - 321 1.9e-09 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0013532)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G09060 AT3G09060
fragaria_vesca FvH4_6g11981
malus_domestica MD04G1147200.v1.1 MD12G1160500.v1.1
prunus_persica Prupe.6G271000_v2.0.a1
pyrus_communis pycom12g15230
rosa_chinensis RchiOBHm_Chr3g0463821
rosa_laevigata RLG00000024754
rosa_multiflora Rmu_sc0004796.1_g000010
rosa_roxburghii Rroxscaffold_6G00416270
rosa_rugosa Rorug03G0068000 Rorug03G0068100
rosa_samantha Rh3AG125000 Rh3BG129300 Rh3CG131900 Rh3DG130400
rosa_wichuraiana Rw3G010560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 666, 774, 939
AatII GACGTC 1 cut(s) 135
AccB7I CCANNNNNTGG 1 cut(s) 387
AccI GTMKAC 1 cut(s) 6
AccII CGCG 1 cut(s) 36
AciI CCGC 9 cut(s) 75, 96, 151, 154, 208, 804, 908, 926, 974
AclWI GGATC 1 cut(s) 339
AcoI YGGCCR 1 cut(s) 582
AcyI GRCGYC 1 cut(s) 132
AfaI GTAC 1 cut(s) 455
AfiI CCNNNNNNNGG 3 cut(s) 387, 591, 638
AjiI CACGTC 3 cut(s) 184, 238, 298
AjnI CCWGG 1 cut(s) 503
AloI GAACNNNNNNTCC 2 cut(s) 56, 88
AluBI AGCT 2 cut(s) 46, 721
AluI AGCT 2 cut(s) 46, 721
Alw26I GTCTC 1 cut(s) 812
AlwI GGATC 1 cut(s) 339
Ama87I CYCGRG 2 cut(s) 189, 230
AoxI GGCC 2 cut(s) 501, 582
ApeKI GCWGC 1 cut(s) 511
ArsI GACNNNNNNTTYG 2 cut(s) 8, 40
AspLEI GCGC 1 cut(s) 36
AspS9I GGNCC 2 cut(s) 391, 742
AsuC2I CCSGG 2 cut(s) 336, 746
AsuHPI GGTGA 2 cut(s) 748, 827
AsuII TTCGAA 1 cut(s) 420
AvaI CYCGRG 2 cut(s) 189, 230
AvaII GGWCC 2 cut(s) 391, 742
BalI TGGCCA 1 cut(s) 584
BanII GRGCYC 1 cut(s) 336
BbsI GAAGAC 2 cut(s) 127, 292
BbvI GCAGC 1 cut(s) 498
BccI CCATC 3 cut(s) 674, 733, 992
BciT130I CCWGG 1 cut(s) 505
BclI TGATCA 2 cut(s) 465, 570
BcnI CCSGG 2 cut(s) 336, 746
BcoDI GTCTC 1 cut(s) 812
BfmI CTRYAG 1 cut(s) 446
BisI GCNGC 2 cut(s) 154, 512
BlsI GCNGC 2 cut(s) 155, 513
Bme1390I CCNGG 3 cut(s) 336, 505, 746
Bme18I GGWCC 2 cut(s) 391, 742
BmeT110I CYCGRG 2 cut(s) 189, 230
BmgBI CACGTC 3 cut(s) 184, 238, 298
BmgT120I GGNCC 2 cut(s) 391, 742
BmiI GGNNCC 3 cut(s) 392, 543, 744
BmrFI CCNGG 3 cut(s) 336, 505, 746
BmsI GCATC 2 cut(s) 595, 961
BpiI GAAGAC 2 cut(s) 127, 292
Bpu10I CCTNAGC 1 cut(s) 714
Bpu14I TTCGAA 1 cut(s) 420
BpuEI CTTGAG 1 cut(s) 26
BpuMI CCSGG 2 cut(s) 336, 746
BsaHI GRCGYC 1 cut(s) 132
BsaJI CCNNGG 2 cut(s) 231, 504
BsaXI ACNNNNNCTCC 8 cut(s) 56, 69, 86, 99, 107, 137, 799, 829
Bsc4I CCNNNNNNNGG 3 cut(s) 387, 591, 638
Bse1I ACTGG 1 cut(s) 385
Bse3DI GCAATG 1 cut(s) 996
BseBI CCWGG 1 cut(s) 505
BseDI CCNNGG 2 cut(s) 231, 504
BseGI GGATG 9 cut(s) 144, 159, 204, 528, 610, 846, 866, 925, 976
BseLI CCNNNNNNNGG 3 cut(s) 387, 591, 638
BseMI GCAATG 1 cut(s) 996
BseMII CTCAG 2 cut(s) 624, 658
BseNI ACTGG 1 cut(s) 385
BseRI GAGGAG 2 cut(s) 38, 77
BseXI GCAGC 1 cut(s) 498
BseYI CCCAGC 1 cut(s) 598
BsgI GTGCAG 1 cut(s) 278
Bsh1236I CGCG 1 cut(s) 36
BshFI GGCC 2 cut(s) 503, 584
BsiHKCI CYCGRG 2 cut(s) 189, 230
BsiSI CCGG 3 cut(s) 336, 746, 968
BslFI GGGAC 3 cut(s) 10, 404, 421
BslI CCNNNNNNNGG 3 cut(s) 387, 591, 638
BsmAI GTCTC 1 cut(s) 812
BsmFI GGGAC 3 cut(s) 10, 404, 421
BsmI GAATGC 2 cut(s) 339, 766
BsnI GGCC 2 cut(s) 503, 584
BsoBI CYCGRG 2 cut(s) 189, 230
Bsp119I TTCGAA 1 cut(s) 420
Bsp1286I GDGCHC 1 cut(s) 336
Bsp143I GATC 3 cut(s) 344, 465, 570
BspACI CCGC 9 cut(s) 75, 96, 151, 154, 208, 804, 908, 926, 974
BspANI GGCC 2 cut(s) 503, 584
BspCNI CTCAG 2 cut(s) 625, 657
BspFNI CGCG 1 cut(s) 36
BspLI GGNNCC 3 cut(s) 392, 543, 744
BspMAI CTGCAG 1 cut(s) 450
BspPI GGATC 1 cut(s) 339
BspT104I TTCGAA 1 cut(s) 420
BsrDI GCAATG 1 cut(s) 996
BsrI ACTGG 1 cut(s) 385
BssECI CCNNGG 2 cut(s) 231, 504
BssMI GATC 3 cut(s) 344, 465, 570
BssNI GRCGYC 1 cut(s) 132
Bst2UI CCWGG 1 cut(s) 505
Bst4CI ACNGT 2 cut(s) 250, 427
Bst6I CTCTTC 1 cut(s) 363
BstACI GRCGYC 1 cut(s) 132
BstBI TTCGAA 1 cut(s) 420
BstC8I GCNNGC 1 cut(s) 332
BstDEI CTNAG 3 cut(s) 633, 644, 714
BstENI CCTNNNNNAGG 1 cut(s) 636
BstF5I GGATG 9 cut(s) 144, 159, 204, 528, 610, 846, 866, 925, 976
BstFNI CGCG 1 cut(s) 36
BstHHI GCGC 1 cut(s) 36
BstKTI GATC 3 cut(s) 347, 468, 573
BstMAI GTCTC 1 cut(s) 812
BstMBI GATC 3 cut(s) 344, 465, 570
BstMWI GCNNNNNNNGC 2 cut(s) 185, 336
BstNI CCWGG 1 cut(s) 505
BstSCI CCNGG 3 cut(s) 334, 503, 744
BstSFI CTRYAG 1 cut(s) 446
BstUI CGCG 1 cut(s) 36
BstV1I GCAGC 1 cut(s) 498
BstV2I GAAGAC 2 cut(s) 127, 292
BsuRI GGCC 2 cut(s) 503, 584
BtrI CACGTC 3 cut(s) 184, 238, 298
BtsCI GGATG 9 cut(s) 144, 159, 204, 528, 610, 846, 866, 925, 976
BtsI GCAGTG 1 cut(s) 223
BtsIMutI CAGTG 3 cut(s) 223, 242, 392
Cac8I GCNNGC 1 cut(s) 332
CfoI GCGC 1 cut(s) 36
Cfr13I GGNCC 2 cut(s) 391, 742
CseI GACGC 1 cut(s) 25
Csp6I GTAC 1 cut(s) 454
CviAII CATG 2 cut(s) 658, 893
CviQI GTAC 1 cut(s) 454
DdeI CTNAG 3 cut(s) 633, 644, 714
DpnI GATC 3 cut(s) 346, 467, 572
DpnII GATC 3 cut(s) 344, 465, 570
EaeI YGGCCR 1 cut(s) 582
Eam1104I CTCTTC 1 cut(s) 363
EarI CTCTTC 1 cut(s) 363
EciI GGCGGA 3 cut(s) 85, 140, 941
Eco24I GRGCYC 1 cut(s) 336
Eco47I GGWCC 2 cut(s) 391, 742
Eco88I CYCGRG 2 cut(s) 189, 230
EcoNI CCTNNNNNAGG 1 cut(s) 636
EcoRII CCWGG 1 cut(s) 503
EcoT22I ATGCAT 1 cut(s) 610
EcoT38I GRGCYC 1 cut(s) 336
FaeI CATG 2 cut(s) 661, 896
FaqI GGGAC 3 cut(s) 10, 404, 421
FatI CATG 2 cut(s) 657, 892
FauI CCCGC 2 cut(s) 797, 901
FbaI TGATCA 2 cut(s) 465, 570
FblI GTMKAC 1 cut(s) 6
Fnu4HI GCNGC 2 cut(s) 154, 512
FokI GGATG 9 cut(s) 131, 146, 191, 535, 617, 853, 873, 932, 983
FriOI GRGCYC 1 cut(s) 336
Fsp4HI GCNGC 2 cut(s) 154, 512
GlaI GCGC 1 cut(s) 35
GluI GCNGC 2 cut(s) 154, 512
GsaI CCCAGC 1 cut(s) 602
HaeIII GGCC 2 cut(s) 503, 584
HapII CCGG 3 cut(s) 336, 746, 968
HgaI GACGC 1 cut(s) 25
HhaI GCGC 1 cut(s) 36
Hin1I GRCGYC 1 cut(s) 132
Hin1II CATG 2 cut(s) 661, 896
Hin6I GCGC 1 cut(s) 34
HinP1I GCGC 1 cut(s) 34
HincII GTYRAC 1 cut(s) 7
HindII GTYRAC 1 cut(s) 7
HinfI GANTC 4 cut(s) 92, 377, 642, 889
HpaII CCGG 3 cut(s) 336, 746, 968
HphI GGTGA 2 cut(s) 748, 827
Hpy166II GTNNAC 2 cut(s) 7, 873
Hpy188I TCNGA 3 cut(s) 465, 693, 759
Hpy188III TCNNGA 3 cut(s) 13, 469, 650
Hpy8I GTNNAC 2 cut(s) 7, 873
Hpy99I CGWCG 5 cut(s) 134, 179, 188, 200, 626
HpyAV CCTTC 4 cut(s) 379, 493, 526, 949
HpyCH4III ACNGT 2 cut(s) 250, 427
HpyCH4IV ACGT 5 cut(s) 132, 183, 237, 297, 452
HpyCH4V TGCA 6 cut(s) 295, 448, 480, 608, 798, 989
HpyF10VI GCNNNNNNNGC 2 cut(s) 185, 336
HpyF3I CTNAG 3 cut(s) 633, 644, 714
HpySE526I ACGT 5 cut(s) 132, 183, 237, 297, 452
Hsp92I GRCGYC 1 cut(s) 132
Hsp92II CATG 2 cut(s) 661, 896
HspAI GCGC 1 cut(s) 34
Ksp22I TGATCA 2 cut(s) 465, 570
Kzo9I GATC 3 cut(s) 344, 465, 570
LmnI GCTCC 3 cut(s) 51, 541, 718
Lsp1109I GCAGC 1 cut(s) 498
LweI GCATC 2 cut(s) 595, 961
MaeII ACGT 5 cut(s) 132, 183, 237, 297, 452
MaeIII GTNAC 1 cut(s) 557
MalI GATC 3 cut(s) 346, 467, 572
MboI GATC 3 cut(s) 344, 465, 570
MboII GAAGA 4 cut(s) 127, 292, 350, 496
MhlI GDGCHC 1 cut(s) 336
MlsI TGGCCA 1 cut(s) 584
MluCI AATT 1 cut(s) 352
MluNI TGGCCA 1 cut(s) 584
MlyI GAGTC 3 cut(s) 86, 386, 636
MmeI TCCRAC 1 cut(s) 405
Mox20I TGGCCA 1 cut(s) 584
Mph1103I ATGCAT 1 cut(s) 610
MscI TGGCCA 1 cut(s) 584
Msp20I TGGCCA 1 cut(s) 584
MspI CCGG 3 cut(s) 336, 746, 968
MspR9I CCNGG 3 cut(s) 336, 505, 746
Mva1269I GAATGC 2 cut(s) 339, 766
MvaI CCWGG 1 cut(s) 505
MvnI CGCG 1 cut(s) 36
MwoI GCNNNNNNNGC 2 cut(s) 185, 336
NciI CCSGG 2 cut(s) 336, 746
NdeII GATC 3 cut(s) 344, 465, 570
NlaIII CATG 2 cut(s) 661, 896
NlaIV GGNNCC 3 cut(s) 392, 543, 744
NmeAIII GCCGAG 1 cut(s) 424
NsiI ATGCAT 1 cut(s) 610
NspV TTCGAA 1 cut(s) 420
PcsI WCGNNNNNNNCGW 3 cut(s) 87, 180, 192
PctI GAATGC 2 cut(s) 339, 766
PfeI GAWTC 1 cut(s) 889
PflFI GACNNNGTC 1 cut(s) 645
PflMI CCANNNNNTGG 1 cut(s) 387
PkrI GCNGC 2 cut(s) 155, 513
PleI GAGTC 3 cut(s) 86, 385, 636
PpsI GAGTC 3 cut(s) 86, 385, 636
PsiI TTATAA 3 cut(s) 666, 774, 939
Psp6I CCWGG 1 cut(s) 503
PspFI CCCAGC 1 cut(s) 598
PspGI CCWGG 1 cut(s) 503
PspN4I GGNNCC 3 cut(s) 392, 543, 744
PspPI GGNCC 2 cut(s) 391, 742
PstI CTGCAG 1 cut(s) 450
PsyI GACNNNGTC 1 cut(s) 645
RsaI GTAC 1 cut(s) 455
RsaNI GTAC 1 cut(s) 454
SalI GTCGAC 1 cut(s) 5
SatI GCNGC 2 cut(s) 154, 512
Sau3AI GATC 3 cut(s) 344, 465, 570
Sau96I GGNCC 2 cut(s) 391, 742
SchI GAGTC 3 cut(s) 86, 386, 636
ScrFI CCNGG 3 cut(s) 336, 505, 746
SduI GDGCHC 1 cut(s) 336
SfaNI GCATC 2 cut(s) 595, 961
SfcI CTRYAG 1 cut(s) 446
SfuI TTCGAA 1 cut(s) 420
SinI GGWCC 2 cut(s) 391, 742
SmlI CTYRAG 1 cut(s) 41
SmoI CTYRAG 1 cut(s) 41
Sse9I AATT 1 cut(s) 352
SsiI CCGC 9 cut(s) 75, 96, 151, 154, 208, 804, 908, 926, 974
SspI AATATT 1 cut(s) 670
StyD4I CCNGG 3 cut(s) 334, 503, 744
TaaI ACNGT 2 cut(s) 250, 427
TaiI ACGT 5 cut(s) 135, 186, 240, 300, 455
TaqI TCGA 7 cut(s) 6, 90, 198, 343, 420, 621, 996
TaqII GACCGA 1 cut(s) 408
TasI AATT 1 cut(s) 352
TauI GCSGC 1 cut(s) 156
TfiI GAWTC 1 cut(s) 889
TscAI CASTG 3 cut(s) 230, 249, 392
TseI GCWGC 1 cut(s) 511
TspDTI ATGAA 3 cut(s) 361, 867, 881
TspGWI ACGGA 1 cut(s) 576
TspRI CASTG 3 cut(s) 230, 249, 392
Tth111I GACNNNGTC 1 cut(s) 645
Van91I CCANNNNNTGG 1 cut(s) 387
VpaK11BI GGWCC 2 cut(s) 391, 742
XagI CCTNNNNNAGG 1 cut(s) 636
XcmI CCANNNNNNNNNTGG 1 cut(s) 511
XmiI GTMKAC 1 cut(s) 6
ZraI GACGTC 1 cut(s) 133
Zsp2I ATGCAT 1 cut(s) 610
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.