Rmu_sc0005327.1_g000015
NAC Family

Inactive rhomboid protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0005327.1
OG0014538
Physical Location & Seq
Reverse (-)
81489 .. 84910
3422 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1077 bp
atggccgccgagccaccgccatcgaagctccacagccagatcgacatcaacgctcctccgccgtgctccgccgacgactcctccttggccaccgaaaccctccgcgaacagaggctctccttcttcgcctcccgctacactcgccgccgccgccgccgccggagcgacacgtggatcatctcgctcttcgtcatcctccacgtcctggccttctacgttgacctcttcggttccctgcttgatgaagtaggagctcttcgacggacttttttgacagagaatcacaaaacttggcgcctcttcacatttccatgtctgcatgctggagccatccaccttgtgatcaacctttgcagtgttgtcttcattggaattcacttggagcaagagttcggaacatggttaatctatgtactgtcttctttcaatggtacattggtggctgcactttttgttgagaaacgcccagcagtgggttcttccggggctctatatgggttacttggagctacactttctgctctgattcagaactgggacttatacaccaacaagttcacagctctcacatcagtattacttgtcgccattggaaatttcctccttggtttgctaccttatgtggataacttttcaaaccttgggggttttctatcagggttcctactcggatctgcgcttttatccagtcctcaagtcagagaagtggtgcaaggaaaaggaggcctcctggacaatgatcttaaaagctctatcaagtcgaggttcaggcagaagctggacagaccagtctatagaagtgtctgtctttttctctttgtacttatgcttgcaggatgtcttgtagcagttttgcagggcattgacatgaaccagtactgcaaatggtgtcgttatgttgactgcttgccttccaaaagatggagctgcagcgaaatggaagctttttgtgagaccatggtgagccatgaacagatgactttgacctgtatgggtaatgggaacttcaaggttcttcctttcaccaacatttctcaagccaggatgaatgacttgtgcagcgtgatatgctcatag

Protein Analysis

358

Amino Acids

40.0

Weight (kDa)

8.67

Isoelectric Point (pI)

45.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rhomboid PF01694 95 - 228 4.6e-29 Rhomboid domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014538)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G77860 AT1G77860
fragaria_vesca FvH4_5g38430
malus_domestica MD08G1173800.v1.1
prunus_persica Prupe.1G507800_v2.0.a1 Prupe.1G507800_v2.0.a1 Prupe.1G507800_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0240461
rosa_laevigata RLG00000000677
rosa_multiflora Rmu_sc0005327.1_g000015
rosa_roxburghii Rroxscaffold_3G00219970
rosa_rugosa Rorug07G0336300
rosa_samantha Rh7AG490600 Rh7BG463300 Rh7CG506800 Rh7DG474400
rosa_wichuraiana Rw7G041120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 788
AccB1I GGYRCC 1 cut(s) 294
AccB7I CCANNNNNTGG 2 cut(s) 205, 921
AccII CGCG 1 cut(s) 105
AclWI GGATC 2 cut(s) 182, 679
AcoI YGGCCR 2 cut(s) 3, 87
AcsI RAATTY 2 cut(s) 372, 595
AcvI CACGTG 1 cut(s) 171
AcyI GRCGYC 1 cut(s) 295
AdeI CACNNNGTG 1 cut(s) 340
AfaI GTAC 4 cut(s) 414, 433, 822, 878
AfiI CCNNNNNNNGG 4 cut(s) 205, 472, 473, 921
AflIII ACRYGT 1 cut(s) 168
AgsI TTSAA 3 cut(s) 427, 636, 1009
AjiI CACGTC 1 cut(s) 202
AjnI CCWGG 3 cut(s) 204, 729, 1040
AloI GAACNNNNNNTCC 2 cut(s) 374, 406
AluBI AGCT 8 cut(s) 28, 254, 509, 563, 750, 778, 927, 944
AluI AGCT 8 cut(s) 28, 254, 509, 563, 750, 778, 927, 944
Alw21I GWGCWC 2 cut(s) 68, 256
Alw26I GTCTC 1 cut(s) 947
AlwI GGATC 2 cut(s) 182, 679
AlwNI CAGNNNCTG 1 cut(s) 778
AoxI GGCC 4 cut(s) 3, 87, 207, 724
ApeKI GCWGC 4 cut(s) 443, 927, 930, 1059
ApoI RAATTY 2 cut(s) 372, 595
AspLEI GCGC 2 cut(s) 297, 679
AsuC2I CCSGG 1 cut(s) 484
AsuHPI GGTGA 2 cut(s) 973, 1015
BaeI ACNNNNGTAYC 2 cut(s) 423, 456
BalI TGGCCA 1 cut(s) 89
BanI GGYRCC 1 cut(s) 294
BanII GRGCYC 2 cut(s) 256, 490
BarI GAAGNNNNNNTAC 2 cut(s) 240, 272
BbrPI CACGTG 1 cut(s) 171
BbsI GAAGAC 2 cut(s) 355, 411
Bbv12I GWGCWC 2 cut(s) 68, 256
BbvI GCAGC 4 cut(s) 430, 914, 942, 1071
BccI CCATC 3 cut(s) 28, 338, 915
BceAI ACGGC 1 cut(s) 46
BciT130I CCWGG 3 cut(s) 206, 731, 1042
BclI TGATCA 1 cut(s) 342
BcnI CCSGG 1 cut(s) 484
BcoDI GTCTC 1 cut(s) 947
BfmI CTRYAG 2 cut(s) 793, 928
BfoI RGCGCY 1 cut(s) 298
BmcAI AGTACT 1 cut(s) 878
Bme1390I CCNGG 4 cut(s) 206, 484, 731, 1042
BmgBI CACGTC 1 cut(s) 202
BmiI GGNNCC 4 cut(s) 232, 296, 328, 662
BmrFI CCNGG 4 cut(s) 206, 484, 731, 1042
BmrI ACTGGG 1 cut(s) 544
BmuI ACTGGG 1 cut(s) 544
BpiI GAAGAC 2 cut(s) 355, 411
BpmI CTGGAG 1 cut(s) 345
BpuEI CTTGAG 2 cut(s) 678, 1020
BpuMI CCSGG 1 cut(s) 484
BsaAI YACGTR 1 cut(s) 171
BsaBI GATNNNNATC 1 cut(s) 44
BsaHI GRCGYC 1 cut(s) 295
BsaI GGTCTC 1 cut(s) 947
BsaJI CCNNGG 5 cut(s) 84, 483, 604, 640, 957
BsaXI ACNNNNNCTCC 4 cut(s) 65, 95, 374, 404
Bsc4I CCNNNNNNNGG 4 cut(s) 205, 472, 473, 921
Bse1I ACTGG 4 cut(s) 539, 687, 788, 874
Bse8I GATNNNNATC 1 cut(s) 44
BseBI CCWGG 3 cut(s) 206, 731, 1042
BseDI CCNNGG 5 cut(s) 84, 483, 604, 640, 957
BseGI GGATG 4 cut(s) 192, 330, 842, 1050
BseJI GATNNNNATC 1 cut(s) 44
BseLI CCNNNNNNNGG 4 cut(s) 205, 472, 473, 921
BseNI ACTGG 4 cut(s) 539, 687, 788, 874
BseRI GAGGAG 2 cut(s) 45, 70
BseXI GCAGC 4 cut(s) 430, 914, 942, 1071
BseYI CCCAGC 1 cut(s) 466
BsgI GTGCAG 1 cut(s) 429
Bsh1236I CGCG 1 cut(s) 105
BshFI GGCC 4 cut(s) 5, 89, 209, 726
BshNI GGYRCC 1 cut(s) 294
BsiHKAI GWGCWC 2 cut(s) 68, 256
BsiSI CCGG 2 cut(s) 160, 483
BslFI GGGAC 1 cut(s) 551
BslI CCNNNNNNNGG 4 cut(s) 205, 472, 473, 921
BsmAI GTCTC 1 cut(s) 947
BsmFI GGGAC 1 cut(s) 551
BsnI GGCC 4 cut(s) 5, 89, 209, 726
Bso31I GGTCTC 1 cut(s) 947
Bsp1286I GDGCHC 3 cut(s) 68, 256, 490
Bsp143I GATC 5 cut(s) 39, 174, 342, 671, 739
Bsp19I CCATGG 1 cut(s) 957
BspANI GGCC 4 cut(s) 5, 89, 209, 726
BspFNI CGCG 1 cut(s) 105
BspLI GGNNCC 4 cut(s) 232, 296, 328, 662
BspMAI CTGCAG 1 cut(s) 932
BspPI GGATC 2 cut(s) 182, 679
BspQI GCTCTTC 2 cut(s) 191, 261
BspT107I GGYRCC 1 cut(s) 294
BspTNI GGTCTC 1 cut(s) 947
BsrI ACTGG 4 cut(s) 539, 687, 788, 874
BssECI CCNNGG 5 cut(s) 84, 483, 604, 640, 957
BssMI GATC 5 cut(s) 39, 174, 342, 671, 739
BssNI GRCGYC 1 cut(s) 295
BssT1I CCWWGG 4 cut(s) 84, 604, 640, 957
Bst2UI CCWGG 3 cut(s) 206, 731, 1042
Bst4CI ACNGT 1 cut(s) 417
Bst6I CTCTTC 4 cut(s) 191, 230, 261, 305
BstACI GRCGYC 1 cut(s) 295
BstBAI YACGTR 1 cut(s) 171
BstC8I GCNNGC 3 cut(s) 321, 831, 908
BstDSI CCRYGG 1 cut(s) 957
BstF5I GGATG 4 cut(s) 192, 330, 842, 1050
BstFNI CGCG 1 cut(s) 105
BstH2I RGCGCY 1 cut(s) 298
BstHHI GCGC 2 cut(s) 297, 679
BstKTI GATC 5 cut(s) 42, 177, 345, 674, 742
BstMAI GTCTC 1 cut(s) 947
BstMBI GATC 5 cut(s) 39, 174, 342, 671, 739
BstMWI GCNNNNNNNGC 7 cut(s) 25, 141, 150, 153, 156, 162, 1068
BstNI CCWGG 3 cut(s) 206, 731, 1042
BstNSI RCATGY 1 cut(s) 323
BstSCI CCNGG 4 cut(s) 204, 482, 729, 1040
BstSFI CTRYAG 2 cut(s) 793, 928
BstUI CGCG 1 cut(s) 105
BstV1I GCAGC 4 cut(s) 430, 914, 942, 1071
BstV2I GAAGAC 2 cut(s) 355, 411
BstX2I RGATCY 1 cut(s) 671
BstYI RGATCY 1 cut(s) 671
BsuRI GGCC 4 cut(s) 5, 89, 209, 726
BtgI CCRYGG 1 cut(s) 957
BtrI CACGTC 1 cut(s) 202
BtsCI GGATG 4 cut(s) 192, 330, 842, 1050
BtsI GCAGTG 2 cut(s) 361, 477
BtsIMutI CAGTG 2 cut(s) 361, 477
Cac8I GCNNGC 3 cut(s) 321, 831, 908
CaiI CAGNNNCTG 1 cut(s) 778
CfoI GCGC 2 cut(s) 297, 679
Csp6I GTAC 4 cut(s) 413, 432, 821, 877
CviAII CATG 6 cut(s) 312, 320, 399, 868, 958, 968
CviQI GTAC 4 cut(s) 413, 432, 821, 877
DinI GGCGCC 1 cut(s) 296
DpnI GATC 5 cut(s) 41, 176, 344, 673, 741
DpnII GATC 5 cut(s) 39, 174, 342, 671, 739
DraIII CACNNNGTG 1 cut(s) 340
DrdI GACNNNNNNGTC 1 cut(s) 788
DseDI GACNNNNNNGTC 1 cut(s) 788
EaeI YGGCCR 2 cut(s) 3, 87
Eam1104I CTCTTC 4 cut(s) 191, 230, 261, 305
EarI CTCTTC 4 cut(s) 191, 230, 261, 305
EciI GGCGGA 2 cut(s) 48, 58
Ecl136II GAGCTC 1 cut(s) 254
Eco130I CCWWGG 4 cut(s) 84, 604, 640, 957
Eco147I AGGCCT 1 cut(s) 726
Eco24I GRGCYC 2 cut(s) 256, 490
Eco31I GGTCTC 1 cut(s) 947
Eco53kI GAGCTC 1 cut(s) 254
Eco72I CACGTG 1 cut(s) 171
EcoICRI GAGCTC 1 cut(s) 254
EcoRI GAATTC 1 cut(s) 372
EcoRII CCWGG 3 cut(s) 204, 729, 1040
EcoT14I CCWWGG 4 cut(s) 84, 604, 640, 957
EcoT38I GRGCYC 2 cut(s) 256, 490
EgeI GGCGCC 1 cut(s) 296
EheI GGCGCC 1 cut(s) 296
ErhI CCWWGG 4 cut(s) 84, 604, 640, 957
FaeI CATG 6 cut(s) 315, 323, 402, 871, 961, 971
FaqI GGGAC 1 cut(s) 551
FatI CATG 6 cut(s) 311, 319, 398, 867, 957, 967
FauI CCCGC 1 cut(s) 140
FbaI TGATCA 1 cut(s) 342
FokI GGATG 4 cut(s) 179, 317, 849, 1057
FriOI GRGCYC 2 cut(s) 256, 490
GlaI GCGC 2 cut(s) 296, 678
GsaI CCCAGC 1 cut(s) 470
GsuI CTGGAG 1 cut(s) 345
HaeII RGCGCY 1 cut(s) 298
HaeIII GGCC 4 cut(s) 5, 89, 209, 726
HapII CCGG 2 cut(s) 160, 483
HhaI GCGC 2 cut(s) 297, 679
Hin1I GRCGYC 1 cut(s) 295
Hin1II CATG 6 cut(s) 315, 323, 402, 871, 961, 971
Hin6I GCGC 2 cut(s) 295, 677
HinP1I GCGC 2 cut(s) 295, 677
HincII GTYRAC 2 cut(s) 220, 901
HindII GTYRAC 2 cut(s) 220, 901
HindIII AAGCTT 1 cut(s) 942
HinfI GANTC 3 cut(s) 77, 280, 526
HpaII CCGG 2 cut(s) 160, 483
HphI GGTGA 2 cut(s) 973, 1015
Hpy166II GTNNAC 3 cut(s) 220, 558, 901
Hpy188I TCNGA 5 cut(s) 395, 525, 531, 671, 701
Hpy8I GTNNAC 3 cut(s) 220, 558, 901
Hpy99I CGWCG 2 cut(s) 77, 264
HpyAV CCTTC 3 cut(s) 130, 220, 921
HpyCH4III ACNGT 1 cut(s) 417
HpyCH4IV ACGT 3 cut(s) 170, 201, 216
HpyCH4V TGCA 9 cut(s) 319, 354, 446, 712, 833, 856, 882, 930, 1059
HpyF10VI GCNNNNNNNGC 7 cut(s) 25, 141, 150, 153, 156, 162, 1068
HpySE526I ACGT 3 cut(s) 170, 201, 216
Hsp92I GRCGYC 1 cut(s) 295
Hsp92II CATG 6 cut(s) 315, 323, 402, 871, 961, 971
HspAI GCGC 2 cut(s) 295, 677
KasI GGCGCC 1 cut(s) 294
Ksp22I TGATCA 1 cut(s) 342
Kzo9I GATC 5 cut(s) 39, 174, 342, 671, 739
LguI GCTCTTC 2 cut(s) 191, 261
LmnI GCTCC 9 cut(s) 33, 58, 71, 162, 251, 326, 382, 506, 924
Lsp1109I GCAGC 4 cut(s) 430, 914, 942, 1071
MaeII ACGT 3 cut(s) 170, 201, 216
MaeIII GTNAC 1 cut(s) 498
MalI GATC 5 cut(s) 41, 176, 344, 673, 741
MboI GATC 5 cut(s) 39, 174, 342, 671, 739
MboII GAAGA 9 cut(s) 115, 178, 217, 248, 292, 355, 411, 471, 1007
MflI RGATCY 1 cut(s) 671
MhlI GDGCHC 3 cut(s) 68, 256, 490
MlsI TGGCCA 1 cut(s) 89
MluCI AATT 2 cut(s) 372, 595
MluNI TGGCCA 1 cut(s) 89
Mly113I GGCGCC 1 cut(s) 295
MlyI GAGTC 1 cut(s) 71
Mox20I TGGCCA 1 cut(s) 89
MscI TGGCCA 1 cut(s) 89
MseI TTAA 2 cut(s) 404, 744
MslI CAYNNNNRTG 2 cut(s) 310, 866
Msp20I TGGCCA 1 cut(s) 89
MspI CCGG 2 cut(s) 160, 483
MspR9I CCNGG 4 cut(s) 206, 484, 731, 1042
MvaI CCWGG 3 cut(s) 206, 731, 1042
MvnI CGCG 1 cut(s) 105
MwoI GCNNNNNNNGC 7 cut(s) 25, 141, 150, 153, 156, 162, 1068
NarI GGCGCC 1 cut(s) 295
NciI CCSGG 1 cut(s) 484
NcoI CCATGG 1 cut(s) 957
NdeII GATC 5 cut(s) 39, 174, 342, 671, 739
NlaIII CATG 6 cut(s) 315, 323, 402, 871, 961, 971
NlaIV GGNNCC 4 cut(s) 232, 296, 328, 662
NmeAIII GCCGAG 1 cut(s) 34
NspI RCATGY 1 cut(s) 323
PaeI GCATGC 1 cut(s) 323
PceI AGGCCT 1 cut(s) 726
PciSI GCTCTTC 2 cut(s) 191, 261
PfeI GAWTC 2 cut(s) 280, 526
PflMI CCANNNNNTGG 2 cut(s) 205, 921
PfoI TCCNGGA 1 cut(s) 729
PleI GAGTC 1 cut(s) 71
PluTI GGCGCC 1 cut(s) 298
PmaCI CACGTG 1 cut(s) 171
PmlI CACGTG 1 cut(s) 171
PpsI GAGTC 1 cut(s) 71
Ppu21I YACGTR 1 cut(s) 171
Psp124BI GAGCTC 1 cut(s) 256
Psp6I CCWGG 3 cut(s) 204, 729, 1040
PspCI CACGTG 1 cut(s) 171
PspFI CCCAGC 1 cut(s) 466
PspGI CCWGG 3 cut(s) 204, 729, 1040
PspN4I GGNNCC 4 cut(s) 232, 296, 328, 662
PstI CTGCAG 1 cut(s) 932
PstNI CAGNNNCTG 1 cut(s) 778
PsuI RGATCY 1 cut(s) 671
RsaI GTAC 4 cut(s) 414, 433, 822, 878
RsaNI GTAC 4 cut(s) 413, 432, 821, 877
RseI CAYNNNNRTG 2 cut(s) 310, 866
SacI GAGCTC 1 cut(s) 256
SapI GCTCTTC 2 cut(s) 191, 261
SaqAI TTAA 2 cut(s) 404, 744
Sau3AI GATC 5 cut(s) 39, 174, 342, 671, 739
ScaI AGTACT 1 cut(s) 878
SchI GAGTC 1 cut(s) 71
ScrFI CCNGG 4 cut(s) 206, 484, 731, 1042
SduI GDGCHC 3 cut(s) 68, 256, 490
SfcI CTRYAG 2 cut(s) 793, 928
SfoI GGCGCC 1 cut(s) 296
SmiMI CAYNNNNRTG 2 cut(s) 310, 866
SmlI CTYRAG 2 cut(s) 693, 1035
SmoI CTYRAG 2 cut(s) 693, 1035
SphI GCATGC 1 cut(s) 323
Sse9I AATT 2 cut(s) 372, 595
SseBI AGGCCT 1 cut(s) 726
SspDI GGCGCC 1 cut(s) 294
SstI GAGCTC 1 cut(s) 256
StuI AGGCCT 1 cut(s) 726
StyD4I CCNGG 4 cut(s) 204, 482, 729, 1040
StyI CCWWGG 4 cut(s) 84, 604, 640, 957
TaaI ACNGT 1 cut(s) 417
TaiI ACGT 3 cut(s) 173, 204, 219
TaqI TCGA 4 cut(s) 23, 42, 259, 761
TasI AATT 2 cut(s) 372, 595
TatI WGTACW 3 cut(s) 412, 820, 876
TauI GCSGC 6 cut(s) 8, 147, 150, 153, 156, 159
TfiI GAWTC 2 cut(s) 280, 526
Tru1I TTAA 2 cut(s) 404, 744
Tru9I TTAA 2 cut(s) 404, 744
TscAI CASTG 2 cut(s) 361, 477
TseI GCWGC 4 cut(s) 443, 927, 930, 1059
TspDTI ATGAA 5 cut(s) 258, 355, 884, 984, 1061
TspGWI ACGGA 1 cut(s) 277
TspRI CASTG 2 cut(s) 361, 477
Van91I CCANNNNNTGG 2 cut(s) 205, 921
XapI RAATTY 2 cut(s) 372, 595
XceI RCATGY 1 cut(s) 323
ZrmI AGTACT 1 cut(s) 878
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.