Rmu_sc0006200.1_g000010

Ankyrin repeat and zinc finger domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0006200.1
OG0007214
Physical Location & Seq
Reverse (-)
56156 .. 60395
4240 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1725 bp
atgtatgaggaatattggaatttacaagtgaagctaagccttgctggaaagaatattgtgaaggaggaggactttgatgagttaacgtctgattcttttaaagactttgatgtctcgagtatatcaggatcagaggatgatgaacctgaaaaagttggccctttcaagctttcaagggcaactgctaggcagaaattactcatccaccttcagacgggagaaagaatttcgatttggaagtgtttgattacaaatgaatctggacctgaaggtaatttgtcatgtgatgaattgttggggagattgaacacggtggttcatgagcctagagataatgcctgttttagagttgtgttgcttgcaagtggtgggcactttgctggttgtgtgtttgatggtagcaagattgtagctcacaagactttccacaggtatgtcgtaagggccaagtctggaaaaaaacagtcatccaatgatgcatctggaaggtttgctcattccgctggagcttctcttcgtcgccataatgaacttgcattgaaaaaggatattcaagagctacttgctgcatggaagccttattttgatgcttcgtcttgcgtttttgtttatgctccttctagtaatcatcagctgctatctaatagggagacaccgtattttagtcatcagaaagtccgaaatattccaatccctattcggagacctactatgaaggaagctcggcggatatatgaacagttgacacaagtctcttatgaacaagaggagaatgaagctcctttagttaccaaaaaggaattgccgtctagttcaggaattgaaaatgatatcatctcaagcatggcgaaagtcaacattgttgagccttttgttgagcctagtacagaagcttgctcaagtactagagagtccaatgaactccctatatcaagtgagagtagtaatgaggttaagggttcgtcaacacctttgcatgaggcagcacagtctgggaatgctctcaaggttttggaactcttggaacagggtttggatccttgtgccagagatgaaaggggacagactccatacacggtagcaaacgaaaaggaggtcagaaacacatttagacggttcatggcctcaaatctagataagtgggattggaatgctgctaaggtacctagtgcattgacaaaggagatggaggaatctcaagctgctaagcaggctgaaaaagaagcgaaaaggaaagctaaagcaaaagaattgaagaaactacgcaaagaaaaagaaaagaaagctcaggctgaggctgctgcttccaagaatacttcgaccattacaaagtcctcttcatcatctcagcccagtcgtgcatcacagatctctaaagaggaggaactgaaaagggccctggtagccgagagggaaaaacgagcagccgcagccgagcagagaatggctgcagctgcagcccttagtgctcaaggtaatagcaccactccacaacccaaaactggggaagctagtgaaattaactgctcttgttgtaatgtgtctctggctggatctgaaggtggcgatggtggctgtggctatggtccaagtgatgctgatggcggtggtaccagggatggtgggggtggtggtggtgttagtggcaagttagtgatggtaattgtggtggtcacaacaatagctctcgagtggtggcagtggcgacgcagacgacaaagatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

574

Amino Acids

63.0

Weight (kDa)

8.33

Isoelectric Point (pI)

51.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
bVLRF1 PF18826 117 - 255 4.9e-47 Bacteroidetes VLRF1 release factor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 110
Acc65I GGTACC 2 cut(s) 1162, 1610
AccB1I GGYRCC 2 cut(s) 1162, 1610
AciI CCGC 4 cut(s) 501, 727, 1428, 1605
AclWI GGATC 4 cut(s) 136, 1031, 1044, 1561
AcsI RAATTY 2 cut(s) 19, 225
AcuI CTGAAG 3 cut(s) 194, 288, 1578
AfaI GTAC 4 cut(s) 886, 904, 1164, 1612
AfiI CCNNNNNNNGG 3 cut(s) 214, 1502, 1503
AgsI TTSAA 7 cut(s) 166, 174, 307, 541, 554, 824, 1255
AjnI CCWGG 2 cut(s) 1398, 1613
AjuI GAANNNNNNNTTGG 2 cut(s) 217, 249
Alw21I GWGCWC 1 cut(s) 1471
Alw26I GTCTC 5 cut(s) 118, 644, 697, 757, 1548
AlwI GGATC 4 cut(s) 136, 1031, 1044, 1561
Ama87I CYCGRG 2 cut(s) 115, 1688
AoxI GGCC 4 cut(s) 157, 444, 1122, 1395
ApaI GGGCCC 1 cut(s) 1399
ApoI RAATTY 2 cut(s) 19, 225
ArsI GACNNNNNNTTYG 2 cut(s) 1080, 1112
Asp718I GGTACC 2 cut(s) 1162, 1610
AspS9I GGNCC 6 cut(s) 158, 263, 444, 1395, 1396, 1586
AvaI CYCGRG 2 cut(s) 115, 1688
AvaII GGWCC 2 cut(s) 263, 1586
BaeGI GKGCMC 2 cut(s) 375, 1399
BamHI GGATCC 1 cut(s) 1036
BanI GGYRCC 2 cut(s) 1162, 1610
BanII GRGCYC 1 cut(s) 1399
Bbv12I GWGCWC 1 cut(s) 1471
BbvCI CCTCAGC 1 cut(s) 1293
BccI CCATC 6 cut(s) 389, 1180, 1562, 1595, 1613, 1651
BceAI ACGGC 1 cut(s) 790
BciT130I CCWGG 2 cut(s) 1400, 1615
BcoDI GTCTC 5 cut(s) 118, 644, 697, 757, 1548
BfaI CTAG 9 cut(s) 186, 327, 621, 810, 882, 906, 1133, 1167, 1512
BfmI CTRYAG 2 cut(s) 1449, 1455
BglII AGATCT 1 cut(s) 1368
BlpI GCTNAGC 2 cut(s) 35, 1206
BmcAI AGTACT 1 cut(s) 904
Bme1390I CCNGG 2 cut(s) 1400, 1615
Bme18I GGWCC 2 cut(s) 263, 1586
BmeT110I CYCGRG 2 cut(s) 115, 1688
BmgT120I GGNCC 6 cut(s) 158, 263, 444, 1395, 1396, 1586
BmiI GGNNCC 4 cut(s) 1038, 1164, 1397, 1612
BmrFI CCNGG 2 cut(s) 1400, 1615
BmrI ACTGGG 2 cut(s) 1347, 1512
BmsI GCATC 5 cut(s) 466, 488, 577, 1370, 1585
BmuI ACTGGG 2 cut(s) 1347, 1512
BoxI GACNNNNGTC 1 cut(s) 749
BpmI CTGGAG 1 cut(s) 525
Bpu10I CCTNAGC 3 cut(s) 1158, 1287, 1293
Bpu1102I GCTNAGC 2 cut(s) 35, 1206
BpuEI CTTGAG 5 cut(s) 823, 883, 989, 1182, 1455
BsaI GGTCTC 1 cut(s) 697
BsaJI CCNNGG 2 cut(s) 1398, 1614
BsaXI ACNNNNNCTCC 2 cut(s) 641, 671
Bsc4I CCNNNNNNNGG 3 cut(s) 214, 1502, 1503
Bse1I ACTGG 2 cut(s) 1353, 1507
BseBI CCWGG 2 cut(s) 1400, 1615
BseDI CCNNGG 2 cut(s) 1398, 1614
BseGI GGATG 4 cut(s) 142, 201, 467, 1624
BseLI CCNNNNNNNGG 3 cut(s) 214, 1502, 1503
BseMII CTCAG 3 cut(s) 1284, 1301, 1361
BseNI ACTGG 2 cut(s) 1353, 1507
BseRI GAGGAG 3 cut(s) 80, 782, 1394
BseSI GKGCMC 2 cut(s) 375, 1399
BshFI GGCC 4 cut(s) 159, 446, 1124, 1397
BshNI GGYRCC 2 cut(s) 1162, 1610
BsiHKAI GWGCWC 1 cut(s) 1471
BsiHKCI CYCGRG 2 cut(s) 115, 1688
BslFI GGGAC 1 cut(s) 1074
BslI CCNNNNNNNGG 3 cut(s) 214, 1502, 1503
BsmAI GTCTC 5 cut(s) 118, 644, 697, 757, 1548
BsmFI GGGAC 1 cut(s) 1074
BsmI GAATGC 2 cut(s) 1003, 1156
BsnI GGCC 4 cut(s) 159, 446, 1124, 1397
Bso31I GGTCTC 1 cut(s) 697
BsoBI CYCGRG 2 cut(s) 115, 1688
Bsp120I GGGCCC 1 cut(s) 1395
Bsp1286I GDGCHC 3 cut(s) 375, 1399, 1471
Bsp143I GATC 4 cut(s) 128, 1036, 1368, 1553
Bsp1720I GCTNAGC 2 cut(s) 35, 1206
BspACI CCGC 4 cut(s) 501, 727, 1428, 1605
BspANI GGCC 4 cut(s) 159, 446, 1124, 1397
BspCNI CTCAG 3 cut(s) 1285, 1300, 1360
BspHI TCATGA 1 cut(s) 319
BspLI GGNNCC 4 cut(s) 1038, 1164, 1397, 1612
BspMAI CTGCAG 2 cut(s) 1453, 1459
BspPI GGATC 4 cut(s) 136, 1031, 1044, 1561
BspT107I GGYRCC 2 cut(s) 1162, 1610
BspTNI GGTCTC 1 cut(s) 697
BsrI ACTGG 2 cut(s) 1353, 1507
BssECI CCNNGG 2 cut(s) 1398, 1614
BssMI GATC 4 cut(s) 128, 1036, 1368, 1553
Bst2UI CCWGG 2 cut(s) 1400, 1615
Bst4CI ACNGT 7 cut(s) 313, 465, 657, 741, 990, 1078, 1116
Bst6I CTCTTC 2 cut(s) 519, 1342
BstC8I GCNNGC 3 cut(s) 360, 895, 1212
BstDEI CTNAG 7 cut(s) 35, 1158, 1206, 1287, 1293, 1347, 1463
BstF5I GGATG 4 cut(s) 142, 201, 467, 1624
BstKTI GATC 4 cut(s) 131, 1039, 1371, 1556
BstMAI GTCTC 5 cut(s) 118, 644, 697, 757, 1548
BstMBI GATC 4 cut(s) 128, 1036, 1368, 1553
BstMWI GCNNNNNNNGC 9 cut(s) 500, 1211, 1298, 1403, 1430, 1454, 1457, 1466, 1572
BstNI CCWGG 2 cut(s) 1400, 1615
BstPAI GACNNNNGTC 1 cut(s) 749
BstSCI CCNGG 2 cut(s) 1398, 1613
BstSFI CTRYAG 2 cut(s) 1449, 1455
BstSLI GKGCMC 2 cut(s) 375, 1399
BstX2I RGATCY 3 cut(s) 1036, 1368, 1553
BstYI RGATCY 3 cut(s) 1036, 1368, 1553
BsuRI GGCC 4 cut(s) 159, 446, 1124, 1397
BtgZI GCGATG 1 cut(s) 1581
BtsCI GGATG 4 cut(s) 142, 201, 467, 1624
BtsI GCAGTG 1 cut(s) 1706
BtsIMutI CAGTG 1 cut(s) 1706
Cac8I GCNNGC 3 cut(s) 360, 895, 1212
CciI TCATGA 1 cut(s) 319
Cfr13I GGNCC 6 cut(s) 158, 263, 444, 1395, 1396, 1586
CseI GACGC 1 cut(s) 1716
Csp6I GTAC 4 cut(s) 885, 903, 1163, 1611
CviAII CATG 6 cut(s) 282, 320, 570, 844, 977, 1120
CviQI GTAC 4 cut(s) 885, 903, 1163, 1611
DdeI CTNAG 7 cut(s) 35, 1158, 1206, 1287, 1293, 1347, 1463
DpnI GATC 4 cut(s) 130, 1038, 1370, 1555
DpnII GATC 4 cut(s) 128, 1036, 1368, 1553
DraI TTTAAA 1 cut(s) 100
DrdI GACNNNNNNGTC 1 cut(s) 110
DseDI GACNNNNNNGTC 1 cut(s) 110
Eam1104I CTCTTC 2 cut(s) 519, 1342
EarI CTCTTC 2 cut(s) 519, 1342
EciI GGCGGA 1 cut(s) 742
Eco24I GRGCYC 1 cut(s) 1399
Eco31I GGTCTC 1 cut(s) 697
Eco32I GATATC 1 cut(s) 832
Eco47I GGWCC 2 cut(s) 263, 1586
Eco57I CTGAAG 3 cut(s) 194, 288, 1578
Eco88I CYCGRG 2 cut(s) 115, 1688
EcoO109I RGGNCCY 2 cut(s) 1395, 1396
EcoRII CCWGG 2 cut(s) 1398, 1613
EcoRV GATATC 1 cut(s) 832
EcoT22I ATGCAT 1 cut(s) 481
EcoT38I GRGCYC 1 cut(s) 1399
FaeI CATG 6 cut(s) 285, 323, 573, 847, 980, 1123
FalI AAGNNNNNCTT 2 cut(s) 546, 578
FaqI GGGAC 1 cut(s) 1074
FatI CATG 6 cut(s) 281, 319, 569, 843, 976, 1119
FokI GGATG 4 cut(s) 149, 188, 454, 1631
FriOI GRGCYC 1 cut(s) 1399
FspBI CTAG 9 cut(s) 186, 327, 621, 810, 882, 906, 1133, 1167, 1512
GsuI CTGGAG 1 cut(s) 525
HaeIII GGCC 4 cut(s) 159, 446, 1124, 1397
HgaI GACGC 1 cut(s) 1716
Hin1II CATG 6 cut(s) 285, 323, 573, 847, 980, 1123
HincII GTYRAC 4 cut(s) 84, 744, 856, 966
HindII GTYRAC 4 cut(s) 84, 744, 856, 966
HindIII AAGCTT 2 cut(s) 167, 891
HinfI GANTC 5 cut(s) 92, 257, 911, 1066, 1193
HpaI GTTAAC 1 cut(s) 84
Hpy166II GTNNAC 4 cut(s) 84, 744, 856, 966
Hpy188I TCNGA 8 cut(s) 91, 133, 213, 672, 680, 702, 1100, 1558
Hpy8I GTNNAC 4 cut(s) 84, 744, 856, 966
Hpy99I CGWCG 2 cut(s) 522, 1710
HpyAV CCTTC 7 cut(s) 55, 218, 263, 480, 627, 709, 1553
HpyCH4III ACNGT 7 cut(s) 313, 465, 657, 741, 990, 1078, 1116
HpyCH4IV ACGT 1 cut(s) 86
HpyCH4V TGCA 9 cut(s) 362, 479, 536, 569, 976, 1172, 1361, 1451, 1457
HpyF10VI GCNNNNNNNGC 9 cut(s) 500, 1211, 1298, 1403, 1430, 1454, 1457, 1466, 1572
HpyF3I CTNAG 7 cut(s) 35, 1158, 1206, 1287, 1293, 1347, 1463
HpySE526I ACGT 1 cut(s) 86
Hsp92II CATG 6 cut(s) 285, 323, 573, 847, 980, 1123
KpnI GGTACC 2 cut(s) 1166, 1614
KspAI GTTAAC 1 cut(s) 84
Kzo9I GATC 4 cut(s) 128, 1036, 1368, 1553
LmnI GCTCC 3 cut(s) 506, 619, 784
LweI GCATC 5 cut(s) 466, 488, 577, 1370, 1585
MaeI CTAG 9 cut(s) 186, 327, 621, 810, 882, 906, 1133, 1167, 1512
MaeII ACGT 1 cut(s) 86
MaeIII GTNAC 2 cut(s) 787, 1672
MalI GATC 4 cut(s) 130, 1038, 1370, 1555
MboI GATC 4 cut(s) 128, 1036, 1368, 1553
MboII GAAGA 3 cut(s) 506, 1267, 1329
MflI RGATCY 3 cut(s) 1036, 1368, 1553
MhlI GDGCHC 3 cut(s) 375, 1399, 1471
MlyI GAGTC 2 cut(s) 920, 1060
Mph1103I ATGCAT 1 cut(s) 481
MseI TTAA 4 cut(s) 83, 99, 954, 1521
MslI CAYNNNNRTG 1 cut(s) 432
MspA1I CMGCKG 3 cut(s) 503, 634, 1454
MspR9I CCNGG 2 cut(s) 1400, 1615
Mva1269I GAATGC 2 cut(s) 1003, 1156
MvaI CCWGG 2 cut(s) 1400, 1615
MwoI GCNNNNNNNGC 9 cut(s) 500, 1211, 1298, 1403, 1430, 1454, 1457, 1466, 1572
NdeII GATC 4 cut(s) 128, 1036, 1368, 1553
NlaIII CATG 6 cut(s) 285, 323, 573, 847, 980, 1123
NlaIV GGNNCC 4 cut(s) 1038, 1164, 1397, 1612
NmeAIII GCCGAG 3 cut(s) 703, 1432, 1459
NmuCI GTSAC 1 cut(s) 1672
NsiI ATGCAT 1 cut(s) 481
PaeR7I CTCGAG 2 cut(s) 115, 1688
PagI TCATGA 1 cut(s) 319
PctI GAATGC 2 cut(s) 1003, 1156
PfeI GAWTC 3 cut(s) 92, 257, 1193
PleI GAGTC 2 cut(s) 919, 1060
PpsI GAGTC 2 cut(s) 919, 1060
PshAI GACNNNNGTC 1 cut(s) 749
Psp6I CCWGG 2 cut(s) 1398, 1613
PspGI CCWGG 2 cut(s) 1398, 1613
PspN4I GGNNCC 4 cut(s) 1038, 1164, 1397, 1612
PspOMI GGGCCC 1 cut(s) 1395
PspPI GGNCC 6 cut(s) 158, 263, 444, 1395, 1396, 1586
PstI CTGCAG 2 cut(s) 1453, 1459
PsuI RGATCY 3 cut(s) 1036, 1368, 1553
PvuII CAGCTG 2 cut(s) 634, 1454
RsaI GTAC 4 cut(s) 886, 904, 1164, 1612
RsaNI GTAC 4 cut(s) 885, 903, 1163, 1611
RseI CAYNNNNRTG 1 cut(s) 432
SaqAI TTAA 4 cut(s) 83, 99, 954, 1521
Sau3AI GATC 4 cut(s) 128, 1036, 1368, 1553
Sau96I GGNCC 6 cut(s) 158, 263, 444, 1395, 1396, 1586
ScaI AGTACT 1 cut(s) 904
SchI GAGTC 2 cut(s) 920, 1060
ScrFI CCNGG 2 cut(s) 1400, 1615
SduI GDGCHC 3 cut(s) 375, 1399, 1471
SfaNI GCATC 5 cut(s) 466, 488, 577, 1370, 1585
SfcI CTRYAG 2 cut(s) 1449, 1455
Sfr274I CTCGAG 2 cut(s) 115, 1688
SinI GGWCC 2 cut(s) 263, 1586
SlaI CTCGAG 2 cut(s) 115, 1688
SmiMI CAYNNNNRTG 1 cut(s) 432
SmlI CTYRAG 7 cut(s) 115, 838, 898, 1004, 1197, 1470, 1688
SmoI CTYRAG 7 cut(s) 115, 838, 898, 1004, 1197, 1470, 1688
SsiI CCGC 4 cut(s) 501, 727, 1428, 1605
SspI AATATT 3 cut(s) 14, 55, 685
SspMI CTAG 9 cut(s) 186, 327, 621, 810, 882, 906, 1133, 1167, 1512
StyD4I CCNGG 2 cut(s) 1398, 1613
TaaI ACNGT 7 cut(s) 313, 465, 657, 741, 990, 1078, 1116
TaiI ACGT 1 cut(s) 89
TaqI TCGA 4 cut(s) 116, 230, 1319, 1689
TatI WGTACW 2 cut(s) 884, 902
TauI GCSGC 1 cut(s) 1430
TfiI GAWTC 3 cut(s) 92, 257, 1193
Tru1I TTAA 4 cut(s) 83, 99, 954, 1521
Tru9I TTAA 4 cut(s) 83, 99, 954, 1521
TscAI CASTG 1 cut(s) 1706
TseFI GTSAC 1 cut(s) 1672
Tsp45I GTSAC 1 cut(s) 1672
TspRI CASTG 1 cut(s) 1706
VpaK11BI GGWCC 2 cut(s) 263, 1586
XapI RAATTY 2 cut(s) 19, 225
XbaI TCTAGA 1 cut(s) 1132
XhoI CTCGAG 2 cut(s) 115, 1688
XspI CTAG 9 cut(s) 186, 327, 621, 810, 882, 906, 1133, 1167, 1512
ZrmI AGTACT 1 cut(s) 904
Zsp2I ATGCAT 1 cut(s) 481
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.