Rmu_sc0006233.1_g000037

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0006233.1
OG0017773
Physical Location & Seq
Forward (+)
154557 .. 155463
907 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 807 bp
atgctggatacgaggaagatccactggaagaattggaatgctctttgtaatccgagggaagagggtggtttgggttttcgtagtctctccaattttaattctgccatgctagcaaaacaagcatggagaatcttgcagaatcctacttctcttattgcctgtatatacaaggcgaaatacttccagaatcattccttttgggaggctttaccccatgattcgccgtcttactcgtggcgaagcattttctccactcgagagctccttcaaggaggctgttactggcaggtgggtaatggtcgagaactggacatcttctcggatcattgggttgtagcgttgcctctggggagacctaccaattttggtgcgacgggggaggttagaaaagtaagtgagcttatgctcgaaactgggagttggaatgatccactgaatgggaggttctcagtcaagactgcatatcggttcgcttttttgaactcttctagttacaatcctttgcagctcccagttgggatgggtttttggaagaagatttggaaagtggttattcctaacaaagctaaaatacatatctggcgcgtgtgtttggatattcttccttctttgggtagcttggcttcgaagagggtggtattggattcgcacaggtgtgtgttgtgtgagactcaacacgaaactacccttcatatatgttgggattgtgtttttacacaagctgtgctccgcaagaatgctctcctaaagcaggtttgctataaccctagtaatatggctgacgatgttcttacttggttggcttag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 RVT_3 zf-RVT

Protein Analysis

268

Amino Acids

30.74

Weight (kDa)

9.24

Isoelectric Point (pI)

33.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RVT PF13966 149 - 241 5.6e-12 zinc-binding in reverse transcriptase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 277
Acc36I ACCTGC 2 cut(s) 277, 742
AccB7I CCANNNNNTGG 1 cut(s) 437
AccII CGCG 1 cut(s) 585
AciI CCGC 1 cut(s) 730
AclWI GGATC 3 cut(s) 13, 330, 422
AfiI CCNNNNNNNGG 3 cut(s) 437, 611, 751
AgsI TTSAA 2 cut(s) 269, 481
AleI CACNNNNGTG 1 cut(s) 654
AluBI AGCT 6 cut(s) 262, 400, 508, 566, 618, 722
AluI AGCT 6 cut(s) 262, 400, 508, 566, 618, 722
Alw21I GWGCWC 2 cut(s) 264, 729
Alw26I GTCTC 3 cut(s) 89, 346, 662
AlwI GGATC 3 cut(s) 13, 330, 422
Ama87I CYCGRG 1 cut(s) 255
ApeKI GCWGC 1 cut(s) 505
Asp700I GAANNNNTTC 1 cut(s) 179
AspLEI GCGC 1 cut(s) 585
AsuII TTCGAA 1 cut(s) 626
AsuNHI GCTAGC 1 cut(s) 109
AvaI CYCGRG 1 cut(s) 255
BanII GRGCYC 1 cut(s) 264
BarI GAAGNNNNNNTAC 2 cut(s) 607, 639
BauI CACGAG 1 cut(s) 232
Bbv12I GWGCWC 2 cut(s) 264, 729
BbvI GCAGC 1 cut(s) 517
BccI CCATC 1 cut(s) 514
BceAI ACGGC 1 cut(s) 208
BcoDI GTCTC 3 cut(s) 89, 346, 662
BfaI CTAG 3 cut(s) 110, 489, 768
BfuAI ACCTGC 2 cut(s) 277, 742
BisI GCNGC 1 cut(s) 506
BlsI GCNGC 1 cut(s) 507
BmeT110I CYCGRG 1 cut(s) 255
BmrI ACTGGG 2 cut(s) 423, 506
BmtI GCTAGC 1 cut(s) 113
BmuI ACTGGG 2 cut(s) 423, 506
Bpu14I TTCGAA 1 cut(s) 626
BsaI GGTCTC 1 cut(s) 346
BsaJI CCNNGG 1 cut(s) 53
Bsc4I CCNNNNNNNGG 3 cut(s) 437, 611, 751
Bse1I ACTGG 5 cut(s) 29, 287, 312, 418, 512
BseDI CCNNGG 1 cut(s) 53
BseGI GGATG 1 cut(s) 525
BseLI CCNNNNNNNGG 3 cut(s) 437, 611, 751
BseMII CTCAG 1 cut(s) 462
BseNI ACTGG 5 cut(s) 29, 287, 312, 418, 512
BseXI GCAGC 1 cut(s) 517
Bsh1236I CGCG 1 cut(s) 585
BsiHKAI GWGCWC 2 cut(s) 264, 729
BsiHKCI CYCGRG 1 cut(s) 255
BslI CCNNNNNNNGG 3 cut(s) 437, 611, 751
BsmAI GTCTC 3 cut(s) 89, 346, 662
BsmI GAATGC 2 cut(s) 43, 742
Bso31I GGTCTC 1 cut(s) 346
BsoBI CYCGRG 1 cut(s) 255
Bsp119I TTCGAA 1 cut(s) 626
Bsp1286I GDGCHC 2 cut(s) 264, 729
Bsp143I GATC 3 cut(s) 18, 322, 427
BspACI CCGC 1 cut(s) 730
BspCNI CTCAG 1 cut(s) 461
BspFNI CGCG 1 cut(s) 585
BspMI ACCTGC 2 cut(s) 277, 742
BspOI GCTAGC 1 cut(s) 113
BspPI GGATC 3 cut(s) 13, 330, 422
BspT104I TTCGAA 1 cut(s) 626
BspTNI GGTCTC 1 cut(s) 346
BsrI ACTGG 5 cut(s) 29, 287, 312, 418, 512
BssECI CCNNGG 1 cut(s) 53
BssMI GATC 3 cut(s) 18, 322, 427
BssSI CACGAG 1 cut(s) 232
Bst2BI CACGAG 1 cut(s) 232
Bst6I CTCTTC 3 cut(s) 54, 490, 623
BstBI TTCGAA 1 cut(s) 626
BstC8I GCNNGC 1 cut(s) 111
BstDEI CTNAG 2 cut(s) 448, 804
BstENI CCTNNNNNAGG 1 cut(s) 749
BstF5I GGATG 1 cut(s) 525
BstFNI CGCG 1 cut(s) 585
BstHHI GCGC 1 cut(s) 585
BstKTI GATC 3 cut(s) 21, 325, 430
BstMAI GTCTC 3 cut(s) 89, 346, 662
BstMBI GATC 3 cut(s) 18, 322, 427
BstMWI GCNNNNNNNGC 2 cut(s) 110, 119
BstUI CGCG 1 cut(s) 585
BstV1I GCAGC 1 cut(s) 517
BstX2I RGATCY 1 cut(s) 18
BstYI RGATCY 1 cut(s) 18
BtsCI GGATG 1 cut(s) 525
BtsIMutI CAGTG 2 cut(s) 22, 431
BveI ACCTGC 2 cut(s) 277, 742
Cac8I GCNNGC 1 cut(s) 111
CfoI GCGC 1 cut(s) 585
CviAII CATG 3 cut(s) 106, 123, 215
DdeI CTNAG 2 cut(s) 448, 804
DpnI GATC 3 cut(s) 20, 324, 429
DpnII GATC 3 cut(s) 18, 322, 427
Eam1104I CTCTTC 3 cut(s) 54, 490, 623
EarI CTCTTC 3 cut(s) 54, 490, 623
Ecl136II GAGCTC 1 cut(s) 262
Eco24I GRGCYC 1 cut(s) 264
Eco31I GGTCTC 1 cut(s) 346
Eco53kI GAGCTC 1 cut(s) 262
Eco88I CYCGRG 1 cut(s) 255
EcoICRI GAGCTC 1 cut(s) 262
EcoNI CCTNNNNNAGG 1 cut(s) 749
EcoT38I GRGCYC 1 cut(s) 264
FaeI CATG 3 cut(s) 109, 126, 218
FatI CATG 3 cut(s) 105, 122, 214
Fnu4HI GCNGC 1 cut(s) 506
FokI GGATG 1 cut(s) 532
FriOI GRGCYC 1 cut(s) 264
Fsp4HI GCNGC 1 cut(s) 506
FspBI CTAG 3 cut(s) 110, 489, 768
GlaI GCGC 1 cut(s) 584
GluI GCNGC 1 cut(s) 506
HhaI GCGC 1 cut(s) 585
Hin1II CATG 3 cut(s) 109, 126, 218
Hin6I GCGC 1 cut(s) 583
HinP1I GCGC 1 cut(s) 583
HinfI GANTC 6 cut(s) 129, 139, 187, 218, 644, 670
Hpy188I TCNGA 2 cut(s) 54, 322
Hpy188III TCNNGA 4 cut(s) 184, 257, 302, 454
Hpy99I CGWCG 1 cut(s) 376
HpyAV CCTTC 3 cut(s) 275, 615, 698
HpyCH4V TGCA 3 cut(s) 136, 461, 505
HpyF10VI GCNNNNNNNGC 2 cut(s) 110, 119
HpyF3I CTNAG 2 cut(s) 448, 804
Hsp92II CATG 3 cut(s) 109, 126, 218
HspAI GCGC 1 cut(s) 583
Kzo9I GATC 3 cut(s) 18, 322, 427
LmnI GCTCC 3 cut(s) 267, 513, 732
Lsp1109I GCAGC 1 cut(s) 517
MaeI CTAG 3 cut(s) 110, 489, 768
MaeIII GTNAC 2 cut(s) 278, 491
MalI GATC 3 cut(s) 20, 324, 429
MboI GATC 3 cut(s) 18, 322, 427
MboII GAAGA 9 cut(s) 28, 40, 71, 307, 477, 544, 547, 593, 640
MflI RGATCY 1 cut(s) 18
MhlI GDGCHC 2 cut(s) 264, 729
MluCI AATT 4 cut(s) 31, 91, 97, 361
MlyI GAGTC 1 cut(s) 664
MmeI TCCRAC 1 cut(s) 401
MnlI CCTC 9 cut(s) 6, 48, 55, 196, 266, 354, 373, 435, 624
MroXI GAANNNNTTC 1 cut(s) 179
MseI TTAA 1 cut(s) 96
MslI CAYNNNNRTG 1 cut(s) 654
Mva1269I GAATGC 2 cut(s) 43, 742
MvnI CGCG 1 cut(s) 585
MwoI GCNNNNNNNGC 2 cut(s) 110, 119
NdeII GATC 3 cut(s) 18, 322, 427
NheI GCTAGC 1 cut(s) 109
NlaIII CATG 3 cut(s) 109, 126, 218
NspV TTCGAA 1 cut(s) 626
OliI CACNNNNGTG 1 cut(s) 654
PaeR7I CTCGAG 1 cut(s) 255
PaqCI CACCTGC 1 cut(s) 277
PctI GAATGC 2 cut(s) 43, 742
PdmI GAANNNNTTC 1 cut(s) 179
PfeI GAWTC 5 cut(s) 129, 139, 187, 218, 644
PflMI CCANNNNNTGG 1 cut(s) 437
PkrI GCNGC 1 cut(s) 507
PleI GAGTC 1 cut(s) 664
PpsI GAGTC 1 cut(s) 664
Psp124BI GAGCTC 1 cut(s) 264
PsuI RGATCY 1 cut(s) 18
RseI CAYNNNNRTG 1 cut(s) 654
SacI GAGCTC 1 cut(s) 264
SaqAI TTAA 1 cut(s) 96
SatI GCNGC 1 cut(s) 506
Sau3AI GATC 3 cut(s) 18, 322, 427
SchI GAGTC 1 cut(s) 664
SduI GDGCHC 2 cut(s) 264, 729
Sfr274I CTCGAG 1 cut(s) 255
SfuI TTCGAA 1 cut(s) 626
SlaI CTCGAG 1 cut(s) 255
SmiMI CAYNNNNRTG 1 cut(s) 654
SmlI CTYRAG 1 cut(s) 255
SmoI CTYRAG 1 cut(s) 255
Sse9I AATT 4 cut(s) 31, 91, 97, 361
SsiI CCGC 1 cut(s) 730
SspMI CTAG 3 cut(s) 110, 489, 768
SstI GAGCTC 1 cut(s) 264
TaqI TCGA 4 cut(s) 256, 301, 408, 626
TasI AATT 4 cut(s) 31, 91, 97, 361
TfiI GAWTC 5 cut(s) 129, 139, 187, 218, 644
Tru1I TTAA 1 cut(s) 96
Tru9I TTAA 1 cut(s) 96
TscAI CASTG 2 cut(s) 29, 438
TseI GCWGC 1 cut(s) 505
TspDTI ATGAA 1 cut(s) 680
TspRI CASTG 2 cut(s) 29, 438
Van91I CCANNNNNTGG 1 cut(s) 437
XagI CCTNNNNNAGG 1 cut(s) 749
XhoI CTCGAG 1 cut(s) 255
XmnI GAANNNNTTC 1 cut(s) 179
XspI CTAG 3 cut(s) 110, 489, 768
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.