Rmu_sc0006413.1_g000025

Copper-transporting ATPase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0006413.1
OG0018103
Physical Location & Seq
Forward (+)
169198 .. 175166
5969 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1416 bp
atgtgtgatggcttgagagcgaagggtggctctgacttccttgggttcgatccccaaaacctccggagggttagttacgccggtcgtccgggtattccgatagtaataacgggcccggtactgtgcactggtgacgggacagtagcggacggagctgggtgtgtggaaccagttcgagatccgcggcactgtgacgtgcttaagctggtgaggtgcagtttggggcgttacagtgttggtggtgccaccaacatggccagaggcgttgcctccctctgtctttccacgttgacctctttggtttcgtgttcgcctattttggcgattaccttcccaagtagagcgattatatggactagaacttccagaagtatccctaagattagggatgttgtcatgcttttcagcgacattcatagcttcaacaagctcatgaaactttgtgatccaacctgtagtatcatcgattcgatagttcttatcaaccatcaatgcatagacggggaagagttgatcggtgctgacgtggcgctgtctgctgctgacgtggctgttgaccacttgttgacatggctgatgatgtggcagagtctgtggcagttcggtaggtttccgaccggttcttggggtggctccgcctgttctggactcctggggtcgagggtttcaaggtgtgaggtggaggcagaagaaacttcaagttatctctgcaatctcactcgaagcttcaaccttcagacaggaaccctcactgaaggaaaacttgctgtttctagaattgcttctttcaagtataaagactcggaaattcttcaaatagctgctgctgtagagagtacaacatcacatccaattgctgatgctatcttaaacaaagccaaatcattggagttgagtatcccagtcacaaaaaggcaattaaccgaaccaggttttggaacattagcagaagtagatggccttttggttgcagtgggttctttggaatgggttcatgagcatttccagagaagaacagatcgatctgacatttcaaaactagagcatgccgtgcatcaatcatcagaaggcataacaccctcaaattattcaaaaacaattgtatatgttggatgtgaaggggaaggtatcatcggtgctattgcaatatccgacagcctgcgggaagatgctgagtttactgtaactagactccagcagaagggtatcagaactgtcttgttttctggggacagggaagaggcagttgcaactacagcaaaattagttggaatagaaaaggaatttatcaagtcatcattgactccacagggaaaatctggagctatttctagtcttaaagctgcagggcatcatgttgcaatggttggtgatggcatacccaacaacaacttgacgaggtctgcattgaagtag

Protein Analysis

471

Amino Acids

50.49

Weight (kDa)

6.6

Isoelectric Point (pI)

35.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase PF00702 247 - 460 4.2e-16 haloacid dehalogenase-like hydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 242
AccB7I CCANNNNNTGG 1 cut(s) 935
AccII CGCG 1 cut(s) 184
AccIII TCCGGA 1 cut(s) 63
AciI CCGC 5 cut(s) 146, 182, 184, 636, 1162
AclWI GGATC 3 cut(s) 44, 173, 440
AcoI YGGCCR 1 cut(s) 255
AcsI RAATTY 2 cut(s) 807, 1283
AcuI CTGAAG 2 cut(s) 719, 774
AfaI GTAC 2 cut(s) 120, 838
AfiI CCNNNNNNNGG 4 cut(s) 67, 624, 935, 1201
AflII CTTAAG 1 cut(s) 200
AgeI ACCGGT 1 cut(s) 617
AgsI TTSAA 9 cut(s) 424, 669, 699, 730, 790, 815, 1035, 1092, 1411
AjiI CACGTC 3 cut(s) 196, 526, 547
AjnI CCWGG 2 cut(s) 651, 928
AjuI GAANNNNNNNTTGG 2 cut(s) 918, 950
AluBI AGCT 8 cut(s) 155, 205, 420, 430, 726, 821, 1325, 1343
AluI AGCT 8 cut(s) 155, 205, 420, 430, 726, 821, 1325, 1343
Alw21I GWGCWC 1 cut(s) 128
Alw44I GTGCAC 1 cut(s) 124
AlwI GGATC 3 cut(s) 44, 173, 440
AlwNI CAGNNNCTG 1 cut(s) 592
Aor13HI TCCGGA 1 cut(s) 63
AoxI GGCC 3 cut(s) 112, 255, 958
ApaI GGGCCC 1 cut(s) 116
ApaLI GTGCAC 1 cut(s) 124
ApeKI GCWGC 4 cut(s) 539, 821, 824, 1343
ApoI RAATTY 2 cut(s) 807, 1283
AsiGI ACCGGT 1 cut(s) 617
Asp700I GAANNNNTTC 4 cut(s) 171, 781, 810, 990
AspLEI GCGC 1 cut(s) 532
AspS9I GGNCC 2 cut(s) 112, 113
AsuC2I CCSGG 2 cut(s) 90, 116
AsuHPI GGTGA 3 cut(s) 143, 220, 1382
BaeGI GKGCMC 2 cut(s) 116, 128
BalI TGGCCA 1 cut(s) 257
BanI GGYRCC 1 cut(s) 242
BanII GRGCYC 1 cut(s) 116
Bbv12I GWGCWC 1 cut(s) 128
BbvI GCAGC 4 cut(s) 526, 808, 811, 1330
BccI CCATC 4 cut(s) 2, 495, 950, 1367
BceAI ACGGC 1 cut(s) 1034
BciT130I CCWGG 2 cut(s) 653, 930
BciVI GTATCC 2 cut(s) 383, 908
BcnI CCSGG 2 cut(s) 90, 116
BfaI CTAG 5 cut(s) 357, 774, 1040, 1188, 1332
BfmI CTRYAG 4 cut(s) 454, 828, 1254, 1344
BfoI RGCGCY 1 cut(s) 533
BfrI CTTAAG 1 cut(s) 200
BfuI GTATCC 2 cut(s) 383, 908
BisI GCNGC 5 cut(s) 185, 540, 822, 825, 1344
BlsI GCNGC 5 cut(s) 186, 541, 823, 826, 1345
Bme1390I CCNGG 4 cut(s) 90, 116, 653, 930
BmgBI CACGTC 3 cut(s) 196, 526, 547
BmgT120I GGNCC 2 cut(s) 112, 113
BmiI GGNNCC 5 cut(s) 114, 168, 244, 634, 745
BmrFI CCNGG 4 cut(s) 90, 116, 653, 930
BmrI ACTGGG 1 cut(s) 896
BmsI GCATC 4 cut(s) 850, 1063, 1159, 1360
BmuI ACTGGG 1 cut(s) 896
BpmI CTGGAG 2 cut(s) 1178, 1341
BpuEI CTTGAG 1 cut(s) 34
BpuMI CCSGG 2 cut(s) 90, 116
Bsa29I ATCGAT 2 cut(s) 465, 1021
BsaJI CCNNGG 3 cut(s) 40, 182, 652
BsaWI WCCGGW 2 cut(s) 63, 617
BsaXI ACNNNNNCTCC 2 cut(s) 58, 88
Bsc4I CCNNNNNNNGG 4 cut(s) 67, 624, 935, 1201
Bse118I RCCGGY 2 cut(s) 80, 617
Bse1I ACTGG 3 cut(s) 133, 170, 902
Bse3DI GCAATG 1 cut(s) 1368
BseAI TCCGGA 1 cut(s) 63
BseBI CCWGG 2 cut(s) 653, 930
BseCI ATCGAT 2 cut(s) 465, 1021
BseDI CCNNGG 3 cut(s) 40, 182, 652
BseGI GGATG 3 cut(s) 394, 847, 1118
BseLI CCNNNNNNNGG 4 cut(s) 67, 624, 935, 1201
BseMI GCAATG 1 cut(s) 1368
BseMII CTCAG 1 cut(s) 1164
BseNI ACTGG 3 cut(s) 133, 170, 902
BseSI GKGCMC 2 cut(s) 116, 128
BseXI GCAGC 4 cut(s) 526, 808, 811, 1330
BseYI CCCAGC 1 cut(s) 155
BsgI GTGCAG 1 cut(s) 235
Bsh1236I CGCG 1 cut(s) 184
Bsh1285I CGRYCG 2 cut(s) 85, 618
BshFI GGCC 3 cut(s) 114, 257, 960
BshNI GGYRCC 1 cut(s) 242
BshTI ACCGGT 1 cut(s) 617
BshVI ATCGAT 2 cut(s) 465, 1021
BsiEI CGRYCG 2 cut(s) 85, 618
BsiHKAI GWGCWC 1 cut(s) 128
BsiSI CCGG 5 cut(s) 64, 81, 89, 116, 618
BslFI GGGAC 2 cut(s) 151, 1244
BslI CCNNNNNNNGG 4 cut(s) 67, 624, 935, 1201
BsmFI GGGAC 2 cut(s) 151, 1244
BsnI GGCC 3 cut(s) 114, 257, 960
Bsp120I GGGCCC 1 cut(s) 112
Bsp1286I GDGCHC 2 cut(s) 116, 128
Bsp13I TCCGGA 1 cut(s) 63
Bsp143I GATC 6 cut(s) 49, 178, 445, 513, 1018, 1022
BspACI CCGC 5 cut(s) 146, 182, 184, 636, 1162
BspANI GGCC 3 cut(s) 114, 257, 960
BspCNI CTCAG 1 cut(s) 1165
BspDI ATCGAT 2 cut(s) 465, 1021
BspEI TCCGGA 1 cut(s) 63
BspFNI CGCG 1 cut(s) 184
BspHI TCATGA 2 cut(s) 432, 994
BspLI GGNNCC 5 cut(s) 114, 168, 244, 634, 745
BspMAI CTGCAG 1 cut(s) 1348
BspPI GGATC 3 cut(s) 44, 173, 440
BspT107I GGYRCC 1 cut(s) 242
BspTI CTTAAG 1 cut(s) 200
BsrDI GCAATG 1 cut(s) 1368
BsrFI RCCGGY 2 cut(s) 80, 617
BsrI ACTGG 3 cut(s) 133, 170, 902
BssAI RCCGGY 2 cut(s) 80, 617
BssECI CCNNGG 3 cut(s) 40, 182, 652
BssMI GATC 6 cut(s) 49, 178, 445, 513, 1018, 1022
BssT1I CCWWGG 1 cut(s) 40
Bst2UI CCWGG 2 cut(s) 653, 930
Bst4CI ACNGT 6 cut(s) 123, 142, 191, 233, 1183, 1216
Bst6I CTCTTC 2 cut(s) 501, 1233
BstAFI CTTAAG 1 cut(s) 200
BstAPI GCANNNNNTGC 1 cut(s) 1051
BstC8I GCNNGC 2 cut(s) 1047, 1160
BstDEI CTNAG 2 cut(s) 378, 1173
BstDSI CCRYGG 1 cut(s) 182
BstF5I GGATG 3 cut(s) 394, 847, 1118
BstFNI CGCG 1 cut(s) 184
BstH2I RGCGCY 1 cut(s) 533
BstHHI GCGC 1 cut(s) 532
BstKTI GATC 6 cut(s) 52, 181, 448, 516, 1021, 1025
BstMBI GATC 6 cut(s) 49, 178, 445, 513, 1018, 1022
BstMCI CGRYCG 2 cut(s) 85, 618
BstMWI GCNNNNNNNGC 6 cut(s) 152, 527, 536, 548, 1051, 1256
BstNI CCWGG 2 cut(s) 653, 930
BstNSI RCATGY 1 cut(s) 1049
BstSCI CCNGG 4 cut(s) 88, 114, 651, 928
BstSFI CTRYAG 4 cut(s) 454, 828, 1254, 1344
BstSLI GKGCMC 2 cut(s) 116, 128
BstUI CGCG 1 cut(s) 184
BstV1I GCAGC 4 cut(s) 526, 808, 811, 1330
BstX2I RGATCY 1 cut(s) 178
BstXI CCANNNNNNTGG 2 cut(s) 253, 886
BstYI RGATCY 1 cut(s) 178
Bsu15I ATCGAT 2 cut(s) 465, 1021
BsuI GTATCC 2 cut(s) 383, 908
BsuRI GGCC 3 cut(s) 114, 257, 960
BsuTUI ATCGAT 2 cut(s) 465, 1021
BtgI CCRYGG 1 cut(s) 182
BtrI CACGTC 3 cut(s) 196, 526, 547
BtsCI GGATG 3 cut(s) 394, 847, 1118
BtsI GCAGTG 1 cut(s) 978
BtsIMutI CAGTG 5 cut(s) 126, 187, 238, 750, 978
Cac8I GCNNGC 2 cut(s) 1047, 1160
CaiI CAGNNNCTG 1 cut(s) 592
CciI TCATGA 2 cut(s) 432, 994
CfoI GCGC 1 cut(s) 532
Cfr10I RCCGGY 2 cut(s) 80, 617
Cfr13I GGNCC 2 cut(s) 112, 113
Cfr42I CCGCGG 1 cut(s) 185
ClaI ATCGAT 2 cut(s) 465, 1021
CsiI ACCWGGT 1 cut(s) 928
Csp6I GTAC 2 cut(s) 119, 837
CspAI ACCGGT 1 cut(s) 617
CviAII CATG 7 cut(s) 253, 397, 433, 570, 995, 1046, 1355
CviQI GTAC 2 cut(s) 119, 837
DdeI CTNAG 2 cut(s) 378, 1173
DpnI GATC 6 cut(s) 51, 180, 447, 515, 1020, 1024
DpnII GATC 6 cut(s) 49, 178, 445, 513, 1018, 1022
EaeI YGGCCR 1 cut(s) 255
Eam1104I CTCTTC 2 cut(s) 501, 1233
EarI CTCTTC 2 cut(s) 501, 1233
EciI GGCGGA 1 cut(s) 625
Eco130I CCWWGG 1 cut(s) 40
Eco24I GRGCYC 1 cut(s) 116
Eco57I CTGAAG 2 cut(s) 719, 774
EcoRII CCWGG 2 cut(s) 651, 928
EcoT14I CCWWGG 1 cut(s) 40
EcoT22I ATGCAT 1 cut(s) 497
EcoT38I GRGCYC 1 cut(s) 116
ErhI CCWWGG 1 cut(s) 40
FaeI CATG 7 cut(s) 256, 400, 436, 573, 998, 1049, 1358
FalI AAGNNNNNCTT 2 cut(s) 747, 779
FaqI GGGAC 2 cut(s) 151, 1244
FatI CATG 7 cut(s) 252, 396, 432, 569, 994, 1045, 1354
FauI CCCGC 1 cut(s) 1155
Fnu4HI GCNGC 5 cut(s) 185, 540, 822, 825, 1344
FokI GGATG 3 cut(s) 401, 834, 1125
FriOI GRGCYC 1 cut(s) 116
Fsp4HI GCNGC 5 cut(s) 185, 540, 822, 825, 1344
FspBI CTAG 5 cut(s) 357, 774, 1040, 1188, 1332
GlaI GCGC 1 cut(s) 531
GluI GCNGC 5 cut(s) 185, 540, 822, 825, 1344
GsaI CCCAGC 1 cut(s) 159
GsuI CTGGAG 2 cut(s) 1178, 1341
HaeII RGCGCY 1 cut(s) 533
HaeIII GGCC 3 cut(s) 114, 257, 960
HapII CCGG 5 cut(s) 64, 81, 89, 116, 618
HhaI GCGC 1 cut(s) 532
Hin1II CATG 7 cut(s) 256, 400, 436, 573, 998, 1049, 1358
Hin6I GCGC 1 cut(s) 530
HinP1I GCGC 1 cut(s) 530
HincII GTYRAC 3 cut(s) 291, 556, 567
HindII GTYRAC 3 cut(s) 291, 556, 567
HindIII AAGCTT 1 cut(s) 724
HinfI GANTC 6 cut(s) 467, 589, 648, 800, 1191, 1303
HpaII CCGG 5 cut(s) 64, 81, 89, 116, 618
HphI GGTGA 3 cut(s) 143, 220, 1382
Hpy166II GTNNAC 5 cut(s) 126, 291, 556, 567, 1179
Hpy188I TCNGA 9 cut(s) 34, 99, 615, 738, 805, 1027, 1066, 1153, 1211
Hpy188III TCNNGA 9 cut(s) 64, 176, 366, 433, 645, 774, 995, 1006, 1320
Hpy8I GTNNAC 5 cut(s) 126, 291, 556, 567, 1179
HpyAV CCTTC 8 cut(s) 16, 340, 743, 749, 1061, 1112, 1118, 1195
HpyCH4III ACNGT 6 cut(s) 123, 142, 191, 233, 1183, 1216
HpyCH4IV ACGT 4 cut(s) 195, 287, 525, 546
HpyF10VI GCNNNNNNNGC 6 cut(s) 152, 527, 536, 548, 1051, 1256
HpyF3I CTNAG 2 cut(s) 378, 1173
HpySE526I ACGT 4 cut(s) 195, 287, 525, 546
Hsp92II CATG 7 cut(s) 256, 400, 436, 573, 998, 1049, 1358
HspAI GCGC 1 cut(s) 530
Kpn2I TCCGGA 1 cut(s) 63
KspI CCGCGG 1 cut(s) 185
Kzo9I GATC 6 cut(s) 49, 178, 445, 513, 1018, 1022
LmnI GCTCC 3 cut(s) 152, 638, 1322
Lsp1109I GCAGC 4 cut(s) 526, 808, 811, 1330
LweI GCATC 4 cut(s) 850, 1063, 1159, 1360
MabI ACCWGGT 1 cut(s) 928
MaeI CTAG 5 cut(s) 357, 774, 1040, 1188, 1332
MaeII ACGT 4 cut(s) 195, 287, 525, 546
MaeIII GTNAC 6 cut(s) 74, 131, 191, 227, 904, 1183
MalI GATC 6 cut(s) 51, 180, 447, 515, 1020, 1024
MboI GATC 6 cut(s) 49, 178, 445, 513, 1018, 1022
MboII GAAGA 6 cut(s) 518, 701, 803, 1023, 1178, 1250
MfeI CAATTG 2 cut(s) 852, 1098
MflI RGATCY 1 cut(s) 178
MhlI GDGCHC 2 cut(s) 116, 128
MlsI TGGCCA 1 cut(s) 257
MluCI AATT 8 cut(s) 777, 807, 852, 917, 1084, 1098, 1262, 1283
MluNI TGGCCA 1 cut(s) 257
MlyI GAGTC 5 cut(s) 598, 642, 794, 1185, 1297
MmeI TCCRAC 5 cut(s) 473, 638, 1090, 1176, 1249
Mox20I TGGCCA 1 cut(s) 257
Mph1103I ATGCAT 1 cut(s) 497
MroI TCCGGA 1 cut(s) 63
MroXI GAANNNNTTC 4 cut(s) 171, 781, 810, 990
MscI TGGCCA 1 cut(s) 257
MseI TTAA 4 cut(s) 201, 869, 920, 1338
MslI CAYNNNNRTG 1 cut(s) 251
Msp20I TGGCCA 1 cut(s) 257
MspA1I CMGCKG 1 cut(s) 184
MspCI CTTAAG 1 cut(s) 200
MspI CCGG 5 cut(s) 64, 81, 89, 116, 618
MspR9I CCNGG 4 cut(s) 90, 116, 653, 930
MunI CAATTG 2 cut(s) 852, 1098
MvaI CCWGG 2 cut(s) 653, 930
MvnI CGCG 1 cut(s) 184
MwoI GCNNNNNNNGC 6 cut(s) 152, 527, 536, 548, 1051, 1256
NciI CCSGG 2 cut(s) 90, 116
NdeII GATC 6 cut(s) 49, 178, 445, 513, 1018, 1022
NlaIII CATG 7 cut(s) 256, 400, 436, 573, 998, 1049, 1358
NlaIV GGNNCC 5 cut(s) 114, 168, 244, 634, 745
NmuCI GTSAC 3 cut(s) 131, 191, 904
NsiI ATGCAT 1 cut(s) 497
NspI RCATGY 1 cut(s) 1049
PaeI GCATGC 1 cut(s) 1049
PagI TCATGA 2 cut(s) 432, 994
PcsI WCGNNNNNNNCGW 1 cut(s) 522
PdmI GAANNNNTTC 4 cut(s) 171, 781, 810, 990
PfeI GAWTC 1 cut(s) 467
PflFI GACNNNGTC 1 cut(s) 1399
PflMI CCANNNNNTGG 1 cut(s) 935
PinAI ACCGGT 1 cut(s) 617
PkrI GCNGC 5 cut(s) 186, 541, 823, 826, 1345
PleI GAGTC 5 cut(s) 597, 642, 794, 1185, 1297
PpsI GAGTC 5 cut(s) 597, 642, 794, 1185, 1297
Psp6I CCWGG 2 cut(s) 651, 928
PspFI CCCAGC 1 cut(s) 155
PspGI CCWGG 2 cut(s) 651, 928
PspN4I GGNNCC 5 cut(s) 114, 168, 244, 634, 745
PspOMI GGGCCC 1 cut(s) 112
PspPI GGNCC 2 cut(s) 112, 113
PstI CTGCAG 1 cut(s) 1348
PstNI CAGNNNCTG 1 cut(s) 592
PsuI RGATCY 1 cut(s) 178
PsyI GACNNNGTC 1 cut(s) 1399
RsaI GTAC 2 cut(s) 120, 838
RsaNI GTAC 2 cut(s) 119, 837
RseI CAYNNNNRTG 1 cut(s) 251
SacII CCGCGG 1 cut(s) 185
SaqAI TTAA 4 cut(s) 201, 869, 920, 1338
SatI GCNGC 5 cut(s) 185, 540, 822, 825, 1344
Sau3AI GATC 6 cut(s) 49, 178, 445, 513, 1018, 1022
Sau96I GGNCC 2 cut(s) 112, 113
SchI GAGTC 5 cut(s) 598, 642, 794, 1185, 1297
ScrFI CCNGG 4 cut(s) 90, 116, 653, 930
SduI GDGCHC 2 cut(s) 116, 128
SexAI ACCWGGT 1 cut(s) 928
SfaNI GCATC 4 cut(s) 850, 1063, 1159, 1360
SfcI CTRYAG 4 cut(s) 454, 828, 1254, 1344
Sfr303I CCGCGG 1 cut(s) 185
SgrBI CCGCGG 1 cut(s) 185
SmiMI CAYNNNNRTG 1 cut(s) 251
SmlI CTYRAG 2 cut(s) 13, 200
SmoI CTYRAG 2 cut(s) 13, 200
SphI GCATGC 1 cut(s) 1049
Sse9I AATT 8 cut(s) 777, 807, 852, 917, 1084, 1098, 1262, 1283
SsiI CCGC 5 cut(s) 146, 182, 184, 636, 1162
SspMI CTAG 5 cut(s) 357, 774, 1040, 1188, 1332
StyD4I CCNGG 4 cut(s) 88, 114, 651, 928
StyI CCWWGG 1 cut(s) 40
TaaI ACNGT 6 cut(s) 123, 142, 191, 233, 1183, 1216
TaiI ACGT 4 cut(s) 198, 290, 528, 549
TaqI TCGA 7 cut(s) 48, 175, 465, 470, 659, 721, 1021
TasI AATT 8 cut(s) 777, 807, 852, 917, 1084, 1098, 1262, 1283
TatI WGTACW 1 cut(s) 836
TauI GCSGC 1 cut(s) 187
TfiI GAWTC 1 cut(s) 467
Tru1I TTAA 4 cut(s) 201, 869, 920, 1338
Tru9I TTAA 4 cut(s) 201, 869, 920, 1338
TscAI CASTG 5 cut(s) 133, 194, 238, 757, 978
TseFI GTSAC 3 cut(s) 131, 191, 904
TseI GCWGC 4 cut(s) 539, 821, 824, 1343
Tsp45I GTSAC 3 cut(s) 131, 191, 904
TspDTI ATGAA 3 cut(s) 404, 449, 983
TspGWI ACGGA 1 cut(s) 165
TspRI CASTG 5 cut(s) 133, 194, 238, 757, 978
Tth111I GACNNNGTC 1 cut(s) 1399
Van91I CCANNNNNTGG 1 cut(s) 935
Vha464I CTTAAG 1 cut(s) 200
VneI GTGCAC 1 cut(s) 124
XapI RAATTY 2 cut(s) 807, 1283
XbaI TCTAGA 1 cut(s) 773
XceI RCATGY 1 cut(s) 1049
XmnI GAANNNNTTC 4 cut(s) 171, 781, 810, 990
XspI CTAG 5 cut(s) 357, 774, 1040, 1188, 1332
Zsp2I ATGCAT 1 cut(s) 497
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.