Rmu_sc0006577.1_g000012

Component of the replication protein A complex (RPA) required for DNA recombination, repair and replication. The activity of RPA is mediated by single-stranded DNA binding and protein interactions. Probably involved in repair of double-strand DNA breaks (DSBs) induced by genotoxic stresses

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0006577.1
OG0017447
Physical Location & Seq
Reverse (-)
68031 .. 68369
339 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 339 bp
atggacgcgaggctaacgaagggggcgatactgaagatcacgacggagtttgttgacgacaactataagcctatcctgcgagtgatctacttgaagcagatccactcacagcacggcggcggcggcggcactgagcggtacaagctggccatgtccgatgggtcctacatccggcaggcgaatctggccgctcacccgaacttcctggttcggcaaggccaccttcacaagggctccgttgtccgcctggatcaattcacctgcaccgcgatccaaggccgcaagtatgtgctagcttgtccttccttttcgaaatttgatgctattggtcaagtttag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03030 ko03420 ko03430 ko03440 ko03460 map03030 map03420 map03430 map03440 map03460
Pfam Domains
Protein Families
REPA_OB_2 Rep-A_N Rep_fac-A_C tRNA_anti-codon zf-CCHC

Protein Analysis

112

Amino Acids

12.45

Weight (kDa)

9.69

Isoelectric Point (pI)

35.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rep-A_N PF04057 5 - 98 9.2e-21 Replication factor-A protein 1, N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 269
Acc36I ACCTGC 1 cut(s) 269
AccBSI CCGCTC 2 cut(s) 136, 191
AccII CGCG 2 cut(s) 8, 269
AciI CCGC 9 cut(s) 117, 120, 123, 126, 136, 189, 244, 267, 280
AclWI GGATC 3 cut(s) 94, 258, 265
AcoI YGGCCR 2 cut(s) 147, 186
AcsI RAATTY 1 cut(s) 314
AcuI CTGAAG 1 cut(s) 53
AfaI GTAC 1 cut(s) 140
AfiI CCNNNNNNNGG 2 cut(s) 171, 229
AgsI TTSAA 1 cut(s) 94
AjnI CCWGG 2 cut(s) 204, 246
AluBI AGCT 2 cut(s) 145, 296
AluI AGCT 2 cut(s) 145, 296
AlwI GGATC 3 cut(s) 94, 258, 265
AoxI GGCC 4 cut(s) 147, 186, 217, 277
ApoI RAATTY 1 cut(s) 314
AspS9I GGNCC 1 cut(s) 162
AsuHPI GGTGA 2 cut(s) 185, 250
AsuII TTCGAA 1 cut(s) 311
AsuNHI GCTAGC 1 cut(s) 292
AvaII GGWCC 1 cut(s) 162
BalI TGGCCA 1 cut(s) 149
BanII GRGCYC 1 cut(s) 236
BccI CCATC 1 cut(s) 152
BceAI ACGGC 1 cut(s) 130
BciT130I CCWGG 2 cut(s) 206, 248
BfaI CTAG 1 cut(s) 293
BfuAI ACCTGC 1 cut(s) 269
BisI GCNGC 6 cut(s) 118, 121, 124, 127, 189, 280
BlsI GCNGC 6 cut(s) 119, 122, 125, 128, 190, 281
Bme1390I CCNGG 2 cut(s) 206, 248
Bme18I GGWCC 1 cut(s) 162
BmgT120I GGNCC 1 cut(s) 162
BmiI GGNNCC 2 cut(s) 163, 235
BmrFI CCNGG 2 cut(s) 206, 248
BmsI GCATC 1 cut(s) 310
BmtI GCTAGC 1 cut(s) 296
Bpu14I TTCGAA 1 cut(s) 311
BsaJI CCNNGG 1 cut(s) 274
BsaXI ACNNNNNCTCC 2 cut(s) 218, 248
Bsc4I CCNNNNNNNGG 2 cut(s) 171, 229
BseBI CCWGG 2 cut(s) 206, 248
BseDI CCNNGG 1 cut(s) 274
BseGI GGATG 1 cut(s) 168
BseLI CCNNNNNNNGG 2 cut(s) 171, 229
BseMII CTCAG 1 cut(s) 123
BsgI GTGCAG 1 cut(s) 247
Bsh1236I CGCG 2 cut(s) 8, 269
BshFI GGCC 4 cut(s) 149, 188, 219, 279
BsiSI CCGG 1 cut(s) 172
BslI CCNNNNNNNGG 2 cut(s) 171, 229
BsnI GGCC 4 cut(s) 149, 188, 219, 279
Bsp119I TTCGAA 1 cut(s) 311
Bsp1286I GDGCHC 1 cut(s) 236
Bsp143I GATC 5 cut(s) 36, 84, 99, 250, 270
BspACI CCGC 9 cut(s) 117, 120, 123, 126, 136, 189, 244, 267, 280
BspANI GGCC 4 cut(s) 149, 188, 219, 279
BspCNI CTCAG 1 cut(s) 124
BspFNI CGCG 2 cut(s) 8, 269
BspLI GGNNCC 2 cut(s) 163, 235
BspMI ACCTGC 1 cut(s) 269
BspOI GCTAGC 1 cut(s) 296
BspPI GGATC 3 cut(s) 94, 258, 265
BspT104I TTCGAA 1 cut(s) 311
BsrBI CCGCTC 2 cut(s) 136, 191
BssECI CCNNGG 1 cut(s) 274
BssMI GATC 5 cut(s) 36, 84, 99, 250, 270
BssT1I CCWWGG 1 cut(s) 274
Bst2UI CCWGG 2 cut(s) 206, 248
BstBI TTCGAA 1 cut(s) 311
BstC8I GCNNGC 3 cut(s) 147, 177, 294
BstDEI CTNAG 1 cut(s) 132
BstENI CCTNNNNNAGG 1 cut(s) 227
BstF5I GGATG 1 cut(s) 168
BstFNI CGCG 2 cut(s) 8, 269
BstKTI GATC 5 cut(s) 39, 87, 102, 253, 273
BstMBI GATC 5 cut(s) 36, 84, 99, 250, 270
BstMWI GCNNNNNNNGC 5 cut(s) 76, 123, 126, 142, 185
BstNI CCWGG 2 cut(s) 206, 248
BstSCI CCNGG 2 cut(s) 204, 246
BstUI CGCG 2 cut(s) 8, 269
BstX2I RGATCY 1 cut(s) 99
BstYI RGATCY 1 cut(s) 99
BsuRI GGCC 4 cut(s) 149, 188, 219, 279
BtsCI GGATG 1 cut(s) 168
BtsIMutI CAGTG 1 cut(s) 129
BveI ACCTGC 1 cut(s) 269
Cac8I GCNNGC 3 cut(s) 147, 177, 294
Cfr13I GGNCC 1 cut(s) 162
CseI GACGC 1 cut(s) 14
Csp6I GTAC 1 cut(s) 139
CviAII CATG 1 cut(s) 151
CviJI RGCY 9 cut(s) 13, 70, 145, 149, 188, 219, 234, 279, 296
CviKI_1 RGCY 9 cut(s) 13, 70, 145, 149, 188, 219, 234, 279, 296
CviQI GTAC 1 cut(s) 139
DdeI CTNAG 1 cut(s) 132
DpnI GATC 5 cut(s) 38, 86, 101, 252, 272
DpnII GATC 5 cut(s) 36, 84, 99, 250, 270
EaeI YGGCCR 2 cut(s) 147, 186
EciI GGCGGA 1 cut(s) 233
Eco130I CCWWGG 1 cut(s) 274
Eco24I GRGCYC 1 cut(s) 236
Eco47I GGWCC 1 cut(s) 162
Eco57I CTGAAG 1 cut(s) 53
EcoNI CCTNNNNNAGG 1 cut(s) 227
EcoO109I RGGNCCY 1 cut(s) 162
EcoRII CCWGG 2 cut(s) 204, 246
EcoT14I CCWWGG 1 cut(s) 274
EcoT38I GRGCYC 1 cut(s) 236
ErhI CCWWGG 1 cut(s) 274
FaeI CATG 1 cut(s) 154
FaiI YATR 3 cut(s) 66, 152, 288
FalI AAGNNNNNCTT 2 cut(s) 207, 239
FatI CATG 1 cut(s) 150
Fnu4HI GCNGC 6 cut(s) 118, 121, 124, 127, 189, 280
FokI GGATG 1 cut(s) 155
FriOI GRGCYC 1 cut(s) 236
Fsp4HI GCNGC 6 cut(s) 118, 121, 124, 127, 189, 280
FspBI CTAG 1 cut(s) 293
GluI GCNGC 6 cut(s) 118, 121, 124, 127, 189, 280
HaeIII GGCC 4 cut(s) 149, 188, 219, 279
HapII CCGG 1 cut(s) 172
HgaI GACGC 1 cut(s) 14
Hin1II CATG 1 cut(s) 154
HincII GTYRAC 1 cut(s) 55
HindII GTYRAC 1 cut(s) 55
HinfI GANTC 1 cut(s) 181
HpaII CCGG 1 cut(s) 172
HphI GGTGA 2 cut(s) 185, 250
Hpy166II GTNNAC 1 cut(s) 55
Hpy188I TCNGA 1 cut(s) 157
Hpy188III TCNNGA 1 cut(s) 40
Hpy8I GTNNAC 1 cut(s) 55
Hpy99I CGWCG 1 cut(s) 46
HpyAV CCTTC 3 cut(s) 13, 233, 312
HpyCH4V TGCA 1 cut(s) 264
HpyF10VI GCNNNNNNNGC 5 cut(s) 76, 123, 126, 142, 185
HpyF3I CTNAG 1 cut(s) 132
Hsp92II CATG 1 cut(s) 154
Kzo9I GATC 5 cut(s) 36, 84, 99, 250, 270
LmnI GCTCC 1 cut(s) 239
LweI GCATC 1 cut(s) 310
MaeI CTAG 1 cut(s) 293
MalI GATC 5 cut(s) 38, 86, 101, 252, 272
MbiI CCGCTC 2 cut(s) 136, 191
MboI GATC 5 cut(s) 36, 84, 99, 250, 270
MboII GAAGA 1 cut(s) 46
MflI RGATCY 1 cut(s) 99
MhlI GDGCHC 1 cut(s) 236
MlsI TGGCCA 1 cut(s) 149
MluCI AATT 2 cut(s) 254, 314
MluNI TGGCCA 1 cut(s) 149
MnlI CCTC 1 cut(s) 3
Mox20I TGGCCA 1 cut(s) 149
MscI TGGCCA 1 cut(s) 149
Msp20I TGGCCA 1 cut(s) 149
MspI CCGG 1 cut(s) 172
MspR9I CCNGG 2 cut(s) 206, 248
MvaI CCWGG 2 cut(s) 206, 248
MvnI CGCG 2 cut(s) 8, 269
MwoI GCNNNNNNNGC 5 cut(s) 76, 123, 126, 142, 185
NdeII GATC 5 cut(s) 36, 84, 99, 250, 270
NheI GCTAGC 1 cut(s) 292
NlaIII CATG 1 cut(s) 154
NlaIV GGNNCC 2 cut(s) 163, 235
NspV TTCGAA 1 cut(s) 311
PaqCI CACCTGC 1 cut(s) 269
PcsI WCGNNNNNNNCGW 1 cut(s) 23
PfeI GAWTC 1 cut(s) 181
PkrI GCNGC 6 cut(s) 119, 122, 125, 128, 190, 281
PpuMI RGGWCCY 1 cut(s) 162
Psp5II RGGWCCY 1 cut(s) 162
Psp6I CCWGG 2 cut(s) 204, 246
PspGI CCWGG 2 cut(s) 204, 246
PspN4I GGNNCC 2 cut(s) 163, 235
PspPI GGNCC 1 cut(s) 162
PspPPI RGGWCCY 1 cut(s) 162
PsuI RGATCY 1 cut(s) 99
RsaI GTAC 1 cut(s) 140
RsaNI GTAC 1 cut(s) 139
SatI GCNGC 6 cut(s) 118, 121, 124, 127, 189, 280
Sau3AI GATC 5 cut(s) 36, 84, 99, 250, 270
Sau96I GGNCC 1 cut(s) 162
ScrFI CCNGG 2 cut(s) 206, 248
SduI GDGCHC 1 cut(s) 236
SetI ASST 4 cut(s) 147, 225, 263, 298
SfaNI GCATC 1 cut(s) 310
SfuI TTCGAA 1 cut(s) 311
SinI GGWCC 1 cut(s) 162
Sse9I AATT 2 cut(s) 254, 314
SsiI CCGC 9 cut(s) 117, 120, 123, 126, 136, 189, 244, 267, 280
SspMI CTAG 1 cut(s) 293
StyD4I CCNGG 2 cut(s) 204, 246
StyI CCWWGG 1 cut(s) 274
TaqI TCGA 1 cut(s) 311
TasI AATT 2 cut(s) 254, 314
TauI GCSGC 6 cut(s) 120, 123, 126, 129, 191, 282
TfiI GAWTC 1 cut(s) 181
TscAI CASTG 1 cut(s) 136
TspGWI ACGGA 2 cut(s) 59, 226
TspRI CASTG 1 cut(s) 136
VpaK11BI GGWCC 1 cut(s) 162
XagI CCTNNNNNAGG 1 cut(s) 227
XapI RAATTY 1 cut(s) 314
XspI CTAG 1 cut(s) 293
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.