Rmu_sc0006872.1_g000026

Alpha/beta hydrolase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0006872.1
OG0006375
Physical Location & Seq
Reverse (-)
160365 .. 166511
6147 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1389 bp
atgccgttttgcgaagtcgcgtcgccgcggaatgccgccgcagtcgacgccaagctcaacgacgccggaaccaaaatttactaccggacttatggccgcgggcccaccaaggtcctcctgatcatcggattggcgggaactcacgattcgtgggggccacagatacaggggctgacggggacggtgacggcgaacgacgatgatagggcggcggaggagtgtggcgacaatgaggctggttttagcggcggagggatcgaggtgtgtgcgtttgataatcggggcatgggtcggagctccgtgccggtgaaaaagtcagaatatacaacaagaacaatggcgaaggatgcaattgctttgatggatcatttggggtggaaacaagcgcatgttttcgggcattcaatgggagctatgatagcttgcaagttagcagcgatggtgcccgacagagttttgtcattggctctgctcaatgtgaccggaggaggtttcgagtgtttaccaaggctcgaccgccaaacgttatctattgctatccgatttttaaaggcaaagacgcctgagcaaagagcagctgttgacttggacacacactattcaaaggggtatcttgaggaatatgttggatccaacacaagaagggcaattttataccaagaatatgtaaaaggtatatcagcaactgggatgcagtctaaccatggttttgaaggtcaactgaatgcatgctggacacataaaatgacaccaactgaaattgaaaagatccgctctggtggatttcttgtttcagtcattcatggcaggcatgatgtcatcgctcaaatatattatgcaaagagactggcgcagaagctgcagcctgttgctagaatggttgatcttcctggagggcatctcgtgagtcatgagaggactgaagaggtcaataatgctcttaatgagttgatcaaagcttcggaagctaagacagagccacatgattggacaaatttgcctgagaaaagttcggggtgggtggttacaaggatgttggttgtcaaaaaaagtaatggtgaggttaatagtgcatctttggcatccagaatggctgctatggcttctgacaaggcagctgggcagacgcagtgggagtttagctgtgacttggaggtagattatgagtctgaagaaattgcttctattgtctacacgacattagctgttgataaggagttgcaaccggacaaagtgaaaaggcagatgtcagtttccaatggaaagctttcagtgcattttgaggcagttgaggcaagatttcttcgagcatcatatagtgcttttgtggatgtattgacacttgccacaaaaacagttgaagaatttggaaaaggaatggagttgtaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1

Protein Analysis

462

Amino Acids

50.55

Weight (kDa)

6.38

Isoelectric Point (pI)

37.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Abhydrolase_1 PF00561 38 - 306 2.9e-23 alpha/beta hydrolase fold
Abhydrolase_6 PF12697 82 - 312 1.1e-11 Alpha/beta hydrolase family
Hydrolase_4 PF12146 82 - 159 6.8e-10 Serine aminopeptidase, S33
Pcc1 PF09341 381 - 454 1.3e-21 Transcription factor Pcc1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 442
AccBSI CCGCTC 1 cut(s) 774
AccI GTMKAC 2 cut(s) 45, 1191
AccII CGCG 3 cut(s) 20, 28, 99
AclI AACGTT 1 cut(s) 524
AclWI GGATC 5 cut(s) 263, 372, 624, 637, 763
AcoI YGGCCR 1 cut(s) 94
AcsI RAATTY 3 cut(s) 75, 994, 1364
AcuI CTGAAG 2 cut(s) 942, 1191
AcyI GRCGYC 3 cut(s) 48, 63, 560
AgsI TTSAA 5 cut(s) 405, 603, 713, 764, 1361
AjnI CCWGG 1 cut(s) 889
Alw21I GWGCWC 1 cut(s) 299
Alw26I GTCTC 1 cut(s) 838
AlwI GGATC 5 cut(s) 263, 372, 624, 637, 763
AlwNI CAGNNNCTG 3 cut(s) 172, 686, 859
AoxI GGCC 3 cut(s) 94, 101, 155
ApaI GGGCCC 1 cut(s) 105
ApeKI GCWGC 6 cut(s) 434, 575, 859, 862, 1094, 1115
ApoI RAATTY 3 cut(s) 75, 994, 1364
Asp700I GAANNNNTTC 1 cut(s) 1267
AspLEI GCGC 2 cut(s) 388, 853
AspS9I GGNCC 4 cut(s) 101, 102, 112, 155
AsuHPI GGTGA 3 cut(s) 196, 319, 1070
AvaII GGWCC 1 cut(s) 112
BaeGI GKGCMC 2 cut(s) 105, 447
BamHI GGATCC 1 cut(s) 629
BanI GGYRCC 1 cut(s) 442
BanII GRGCYC 2 cut(s) 105, 299
BauI CACGAG 1 cut(s) 902
Bbv12I GWGCWC 1 cut(s) 299
BbvI GCAGC 6 cut(s) 446, 587, 846, 874, 1081, 1127
BccI CCATC 2 cut(s) 355, 433
BceAI ACGGC 1 cut(s) 204
BcgI CGANNNNNNTGC 2 cut(s) 248, 282
BciT130I CCWGG 1 cut(s) 891
BclI TGATCA 2 cut(s) 120, 951
BcoDI GTCTC 1 cut(s) 838
BfaI CTAG 1 cut(s) 873
BfmI CTRYAG 1 cut(s) 860
Bme1390I CCNGG 1 cut(s) 891
Bme18I GGWCC 1 cut(s) 112
BmgT120I GGNCC 4 cut(s) 101, 102, 112, 155
BmiI GGNNCC 5 cut(s) 70, 103, 156, 444, 631
BmrFI CCNGG 1 cut(s) 891
BmrI ACTGGG 1 cut(s) 696
BmsI GCATC 6 cut(s) 337, 681, 907, 1082, 1091, 1319
BmuI ACTGGG 1 cut(s) 696
BplI GAGNNNNNCTC 2 cut(s) 885, 917
BpmI CTGGAG 1 cut(s) 912
Bpu10I CCTNAGC 1 cut(s) 564
BpuEI CTTGAG 1 cut(s) 635
BsaHI GRCGYC 3 cut(s) 48, 63, 560
BsaJI CCNNGG 5 cut(s) 26, 97, 108, 506, 703
BsaWI WCCGGW 3 cut(s) 84, 482, 1225
Bse118I RCCGGY 1 cut(s) 304
Bse1I ACTGG 2 cut(s) 691, 852
BseBI CCWGG 1 cut(s) 891
BseDI CCNNGG 5 cut(s) 26, 97, 108, 506, 703
BseGI GGATG 5 cut(s) 352, 696, 1038, 1082, 1336
BseMII CTCAG 2 cut(s) 555, 993
BseNI ACTGG 2 cut(s) 691, 852
BseRI GAGGAG 2 cut(s) 230, 501
BseSI GKGCMC 2 cut(s) 105, 447
BseXI GCAGC 6 cut(s) 446, 587, 846, 874, 1081, 1127
BseYI CCCAGC 1 cut(s) 1118
Bsh1236I CGCG 3 cut(s) 20, 28, 99
Bsh1285I CGRYCG 1 cut(s) 517
BshFI GGCC 3 cut(s) 96, 103, 157
BshNI GGYRCC 1 cut(s) 442
BsiEI CGRYCG 1 cut(s) 517
BsiHKAI GWGCWC 1 cut(s) 299
BsiSI CCGG 5 cut(s) 66, 85, 305, 483, 1226
BslFI GGGAC 1 cut(s) 193
BsmAI GTCTC 1 cut(s) 838
BsmFI GGGAC 1 cut(s) 193
BsmI GAATGC 3 cut(s) 37, 400, 730
BsnI GGCC 3 cut(s) 96, 103, 157
Bsp120I GGGCCC 1 cut(s) 101
Bsp1286I GDGCHC 3 cut(s) 105, 299, 447
Bsp143I GATC 7 cut(s) 120, 255, 364, 629, 768, 883, 951
Bsp19I CCATGG 1 cut(s) 703
BspANI GGCC 3 cut(s) 96, 103, 157
BspCNI CTCAG 2 cut(s) 556, 994
BspFNI CGCG 3 cut(s) 20, 28, 99
BspHI TCATGA 1 cut(s) 910
BspLI GGNNCC 5 cut(s) 70, 103, 156, 444, 631
BspMAI CTGCAG 1 cut(s) 864
BspPI GGATC 5 cut(s) 263, 372, 624, 637, 763
BspT107I GGYRCC 1 cut(s) 442
BsrBI CCGCTC 1 cut(s) 774
BsrFI RCCGGY 1 cut(s) 304
BsrI ACTGG 2 cut(s) 691, 852
BssAI RCCGGY 1 cut(s) 304
BssECI CCNNGG 5 cut(s) 26, 97, 108, 506, 703
BssMI GATC 7 cut(s) 120, 255, 364, 629, 768, 883, 951
BssNI GRCGYC 3 cut(s) 48, 63, 560
BssSI CACGAG 1 cut(s) 902
BssT1I CCWWGG 3 cut(s) 108, 506, 703
Bst2BI CACGAG 1 cut(s) 902
Bst2UI CCWGG 1 cut(s) 891
Bst4CI ACNGT 2 cut(s) 184, 1357
Bst6I CTCTTC 1 cut(s) 918
BstACI GRCGYC 3 cut(s) 48, 63, 560
BstAPI GCANNNNNTGC 1 cut(s) 859
BstC8I GCNNGC 4 cut(s) 101, 424, 730, 809
BstDEI CTNAG 3 cut(s) 564, 969, 1002
BstDSI CCRYGG 3 cut(s) 26, 97, 703
BstF5I GGATG 5 cut(s) 352, 696, 1038, 1082, 1336
BstFNI CGCG 3 cut(s) 20, 28, 99
BstHHI GCGC 2 cut(s) 388, 853
BstKTI GATC 7 cut(s) 123, 258, 367, 632, 771, 886, 954
BstMAI GTCTC 1 cut(s) 838
BstMBI GATC 7 cut(s) 120, 255, 364, 629, 768, 883, 951
BstMCI CGRYCG 1 cut(s) 517
BstMWI GCNNNNNNNGC 9 cut(s) 47, 347, 419, 859, 965, 1079, 1100, 1273, 1292
BstNI CCWGG 1 cut(s) 891
BstNSI RCATGY 2 cut(s) 392, 732
BstSCI CCNGG 1 cut(s) 889
BstSFI CTRYAG 1 cut(s) 860
BstSLI GKGCMC 2 cut(s) 105, 447
BstUI CGCG 3 cut(s) 20, 28, 99
BstV1I GCAGC 6 cut(s) 446, 587, 846, 874, 1081, 1127
BstX2I RGATCY 2 cut(s) 629, 768
BstXI CCANNNNNNTGG 1 cut(s) 987
BstYI RGATCY 2 cut(s) 629, 768
BsuRI GGCC 3 cut(s) 96, 103, 157
BtgI CCRYGG 3 cut(s) 26, 97, 703
BtgZI GCGATG 2 cut(s) 452, 805
BtsCI GGATG 5 cut(s) 352, 696, 1038, 1082, 1336
BtsI GCAGTG 1 cut(s) 1136
BtsIMutI CAGTG 2 cut(s) 1136, 1278
Cac8I GCNNGC 4 cut(s) 101, 424, 730, 809
CaiI CAGNNNCTG 3 cut(s) 172, 686, 859
CciI TCATGA 1 cut(s) 910
CfoI GCGC 2 cut(s) 388, 853
Cfr10I RCCGGY 1 cut(s) 304
Cfr13I GGNCC 4 cut(s) 101, 102, 112, 155
Cfr42I CCGCGG 2 cut(s) 29, 100
CseI GACGC 5 cut(s) 9, 56, 71, 568, 1135
CviAII CATG 8 cut(s) 286, 389, 704, 729, 803, 812, 911, 983
DdeI CTNAG 3 cut(s) 564, 969, 1002
DpnI GATC 7 cut(s) 122, 257, 366, 631, 770, 885, 953
DpnII GATC 7 cut(s) 120, 255, 364, 629, 768, 883, 951
DraI TTTAAA 1 cut(s) 549
EaeI YGGCCR 1 cut(s) 94
Eam1104I CTCTTC 1 cut(s) 918
EarI CTCTTC 1 cut(s) 918
EciI GGCGGA 2 cut(s) 227, 264
Ecl136II GAGCTC 1 cut(s) 297
Eco130I CCWWGG 3 cut(s) 108, 506, 703
Eco24I GRGCYC 2 cut(s) 105, 299
Eco47I GGWCC 1 cut(s) 112
Eco53kI GAGCTC 1 cut(s) 297
Eco57I CTGAAG 2 cut(s) 942, 1191
EcoICRI GAGCTC 1 cut(s) 297
EcoO109I RGGNCCY 1 cut(s) 112
EcoRII CCWGG 1 cut(s) 889
EcoT14I CCWWGG 3 cut(s) 108, 506, 703
EcoT22I ATGCAT 1 cut(s) 730
EcoT38I GRGCYC 2 cut(s) 105, 299
ErhI CCWWGG 3 cut(s) 108, 506, 703
FaeI CATG 8 cut(s) 289, 392, 707, 732, 806, 815, 914, 986
FaqI GGGAC 1 cut(s) 193
FatI CATG 8 cut(s) 285, 388, 703, 728, 802, 811, 910, 982
FauI CCCGC 2 cut(s) 92, 127
FbaI TGATCA 2 cut(s) 120, 951
FblI GTMKAC 2 cut(s) 45, 1191
FokI GGATG 5 cut(s) 359, 703, 1045, 1069, 1343
FriOI GRGCYC 2 cut(s) 105, 299
FspBI CTAG 1 cut(s) 873
GlaI GCGC 2 cut(s) 387, 852
GsaI CCCAGC 1 cut(s) 1122
GsuI CTGGAG 1 cut(s) 912
HaeIII GGCC 3 cut(s) 96, 103, 157
HapII CCGG 5 cut(s) 66, 85, 305, 483, 1226
HgaI GACGC 5 cut(s) 9, 56, 71, 568, 1135
HhaI GCGC 2 cut(s) 388, 853
Hin1I GRCGYC 3 cut(s) 48, 63, 560
Hin1II CATG 8 cut(s) 289, 392, 707, 732, 806, 815, 914, 986
Hin6I GCGC 2 cut(s) 386, 851
HinP1I GCGC 2 cut(s) 386, 851
HincII GTYRAC 3 cut(s) 46, 583, 719
HindII GTYRAC 3 cut(s) 46, 583, 719
HindIII AAGCTT 2 cut(s) 957, 1265
HinfI GANTC 3 cut(s) 146, 907, 1166
HpaII CCGG 5 cut(s) 66, 85, 305, 483, 1226
HphI GGTGA 3 cut(s) 196, 319, 1070
Hpy166II GTNNAC 5 cut(s) 46, 503, 583, 719, 1192
Hpy188I TCNGA 7 cut(s) 128, 294, 319, 542, 964, 1108, 1171
Hpy188III TCNNGA 6 cut(s) 118, 143, 614, 904, 911, 1086
Hpy8I GTNNAC 5 cut(s) 46, 503, 583, 719, 1192
Hpy99I CGWCG 4 cut(s) 25, 50, 65, 200
HpyAV CCTTC 3 cut(s) 337, 636, 707
HpyCH4III ACNGT 2 cut(s) 184, 1357
HpyCH4IV ACGT 1 cut(s) 524
HpyCH4V TGCA 9 cut(s) 350, 426, 694, 728, 839, 862, 1073, 1222, 1276
HpyF10VI GCNNNNNNNGC 9 cut(s) 47, 347, 419, 859, 965, 1079, 1100, 1273, 1292
HpyF3I CTNAG 3 cut(s) 564, 969, 1002
HpySE526I ACGT 1 cut(s) 524
Hsp92I GRCGYC 3 cut(s) 48, 63, 560
Hsp92II CATG 8 cut(s) 289, 392, 707, 732, 806, 815, 914, 986
HspAI GCGC 2 cut(s) 386, 851
Ksp22I TGATCA 2 cut(s) 120, 951
KspI CCGCGG 2 cut(s) 29, 100
Kzo9I GATC 7 cut(s) 120, 255, 364, 629, 768, 883, 951
LmnI GCTCC 3 cut(s) 294, 302, 410
Lsp1109I GCAGC 6 cut(s) 446, 587, 846, 874, 1081, 1127
LweI GCATC 6 cut(s) 337, 681, 907, 1082, 1091, 1319
MaeI CTAG 1 cut(s) 873
MaeII ACGT 1 cut(s) 524
MaeIII GTNAC 4 cut(s) 184, 478, 1024, 1145
MalI GATC 7 cut(s) 122, 257, 366, 631, 770, 885, 953
MbiI CCGCTC 1 cut(s) 774
MboI GATC 7 cut(s) 120, 255, 364, 629, 768, 883, 951
MboII GAAGA 5 cut(s) 878, 935, 1184, 1295, 1373
MfeI CAATTG 1 cut(s) 351
MflI RGATCY 2 cut(s) 629, 768
MhlI GDGCHC 3 cut(s) 105, 299, 447
MluCI AATT 7 cut(s) 75, 351, 648, 759, 994, 1176, 1364
MlyI GAGTC 2 cut(s) 916, 1175
MmeI TCCRAC 3 cut(s) 272, 607, 657
Mph1103I ATGCAT 1 cut(s) 730
MroXI GAANNNNTTC 1 cut(s) 1267
MseI TTAA 3 cut(s) 548, 942, 1065
MspA1I CMGCKG 4 cut(s) 28, 99, 578, 1118
MspI CCGG 5 cut(s) 66, 85, 305, 483, 1226
MspR9I CCNGG 1 cut(s) 891
MunI CAATTG 1 cut(s) 351
Mva1269I GAATGC 3 cut(s) 37, 400, 730
MvaI CCWGG 1 cut(s) 891
MvnI CGCG 3 cut(s) 20, 28, 99
MwoI GCNNNNNNNGC 9 cut(s) 47, 347, 419, 859, 965, 1079, 1100, 1273, 1292
NcoI CCATGG 1 cut(s) 703
NdeII GATC 7 cut(s) 120, 255, 364, 629, 768, 883, 951
NlaIII CATG 8 cut(s) 289, 392, 707, 732, 806, 815, 914, 986
NlaIV GGNNCC 5 cut(s) 70, 103, 156, 444, 631
NmuCI GTSAC 3 cut(s) 184, 478, 1145
NsiI ATGCAT 1 cut(s) 730
NspI RCATGY 2 cut(s) 392, 732
PaeI GCATGC 1 cut(s) 732
PagI TCATGA 1 cut(s) 910
PcsI WCGNNNNNNNCGW 1 cut(s) 188
PctI GAATGC 3 cut(s) 37, 400, 730
PdmI GAANNNNTTC 1 cut(s) 1267
PfeI GAWTC 1 cut(s) 146
PfoI TCCNGGA 1 cut(s) 889
PleI GAGTC 2 cut(s) 915, 1174
PpsI GAGTC 2 cut(s) 915, 1174
PpuMI RGGWCCY 1 cut(s) 112
Psp124BI GAGCTC 1 cut(s) 299
Psp1406I AACGTT 1 cut(s) 524
Psp5II RGGWCCY 1 cut(s) 112
Psp6I CCWGG 1 cut(s) 889
PspFI CCCAGC 1 cut(s) 1118
PspGI CCWGG 1 cut(s) 889
PspN4I GGNNCC 5 cut(s) 70, 103, 156, 444, 631
PspOMI GGGCCC 1 cut(s) 101
PspPI GGNCC 4 cut(s) 101, 102, 112, 155
PspPPI RGGWCCY 1 cut(s) 112
PstI CTGCAG 1 cut(s) 864
PstNI CAGNNNCTG 3 cut(s) 172, 686, 859
PsuI RGATCY 2 cut(s) 629, 768
PvuII CAGCTG 2 cut(s) 578, 1118
SacI GAGCTC 1 cut(s) 299
SacII CCGCGG 2 cut(s) 29, 100
SalI GTCGAC 1 cut(s) 44
SaqAI TTAA 3 cut(s) 548, 942, 1065
Sau3AI GATC 7 cut(s) 120, 255, 364, 629, 768, 883, 951
Sau96I GGNCC 4 cut(s) 101, 102, 112, 155
SchI GAGTC 2 cut(s) 916, 1175
ScrFI CCNGG 1 cut(s) 891
SduI GDGCHC 3 cut(s) 105, 299, 447
SfaNI GCATC 6 cut(s) 337, 681, 907, 1082, 1091, 1319
SfcI CTRYAG 1 cut(s) 860
Sfr303I CCGCGG 2 cut(s) 29, 100
SgrBI CCGCGG 2 cut(s) 29, 100
SinI GGWCC 1 cut(s) 112
SmlI CTYRAG 1 cut(s) 614
SmoI CTYRAG 1 cut(s) 614
SphI GCATGC 1 cut(s) 732
Sse9I AATT 7 cut(s) 75, 351, 648, 759, 994, 1176, 1364
SspMI CTAG 1 cut(s) 873
SstI GAGCTC 1 cut(s) 299
StyD4I CCNGG 1 cut(s) 889
StyI CCWWGG 3 cut(s) 108, 506, 703
TaaI ACNGT 2 cut(s) 184, 1357
TaiI ACGT 1 cut(s) 527
TaqI TCGA 5 cut(s) 45, 258, 495, 513, 1306
TasI AATT 7 cut(s) 75, 351, 648, 759, 994, 1176, 1364
TauI GCSGC 6 cut(s) 28, 38, 41, 99, 212, 249
TfiI GAWTC 1 cut(s) 146
Tru1I TTAA 3 cut(s) 548, 942, 1065
Tru9I TTAA 3 cut(s) 548, 942, 1065
TscAI CASTG 2 cut(s) 1136, 1278
TseFI GTSAC 3 cut(s) 184, 478, 1145
TseI GCWGC 6 cut(s) 434, 575, 859, 862, 1094, 1115
Tsp45I GTSAC 3 cut(s) 184, 478, 1145
TspDTI ATGAA 1 cut(s) 791
TspGWI ACGGA 1 cut(s) 289
TspRI CASTG 2 cut(s) 1136, 1278
VpaK11BI GGWCC 1 cut(s) 112
XapI RAATTY 3 cut(s) 75, 994, 1364
XceI RCATGY 2 cut(s) 392, 732
XmiI GTMKAC 2 cut(s) 45, 1191
XmnI GAANNNNTTC 1 cut(s) 1267
XspI CTAG 1 cut(s) 873
Zsp2I ATGCAT 1 cut(s) 730
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.