Rmu_sc0008320.1_g000002

poly ADP-ribose polymerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0008320.1
OG0018229
Physical Location & Seq
Forward (+)
2008 .. 3062
1055 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0008320.1_g000002.1.cds

Sequence Viewer

Length: 564 bp
atgcatccccattcccagatccggattgacactgatgattatcaccaggaggaggaggttcctatgatcgtcgaggatcaagattcctggtcagatcgccccgaatccaattcctccgataccgatgattttggggtttttaccggaaattggatgatgttcaaagtgaatgatgtgagtgaggagtacgatagtattgtcaagagctttgatgtgggtatgggatcgacgggcggagctacgaaggttgtggaggtgcacaagaaatcgatttctggactgatcgggctggccagcttgaaaaatgaaggagaagaacgacaccggcggcggcgggtatgtgagggcggaccagatagatctgaagagcttggacgagcagcttcagaagcatctgagcagggcatgtactatggagaagaacaagaacatagaccagcaggaacaacaaatccgaatattagatatgcttggtacagcggatcaagggatgagattgtggagataatggatcatggatttaatagtcataccatgaaattcaaagaaatctatttattataa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PARP RST

Protein Analysis

187

Amino Acids

21.27

Weight (kDa)

4.78

Isoelectric Point (pI)

56.96

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 562
AccIII TCCGGA 1 cut(s) 21
AciI CCGC 6 cut(s) 234, 328, 331, 334, 348, 480
AclWI GGATC 5 cut(s) 13, 84, 232, 490, 519
AcoI YGGCCR 1 cut(s) 291
AcsI RAATTY 1 cut(s) 539
AcuI CTGAAG 2 cut(s) 369, 384
AfaI GTAC 3 cut(s) 188, 410, 476
AfiI CCNNNNNNNGG 3 cut(s) 21, 52, 150
AgsI TTSAA 3 cut(s) 163, 301, 544
AjnI CCWGG 2 cut(s) 45, 86
AloI GAACNNNNNNTCC 2 cut(s) 436, 468
AluBI AGCT 5 cut(s) 207, 239, 297, 370, 383
AluI AGCT 5 cut(s) 207, 239, 297, 370, 383
Alw21I GWGCWC 1 cut(s) 261
Alw44I GTGCAC 1 cut(s) 257
AlwI GGATC 5 cut(s) 13, 84, 232, 490, 519
Aor13HI TCCGGA 1 cut(s) 21
AoxI GGCC 1 cut(s) 291
ApaLI GTGCAC 1 cut(s) 257
ApeKI GCWGC 1 cut(s) 380
ApoI RAATTY 1 cut(s) 539
AspS9I GGNCC 1 cut(s) 350
AsuHPI GGTGA 1 cut(s) 35
AvaII GGWCC 1 cut(s) 350
BaeGI GKGCMC 1 cut(s) 261
BalI TGGCCA 1 cut(s) 293
Bbv12I GWGCWC 1 cut(s) 261
BbvI GCAGC 1 cut(s) 392
BciT130I CCWGG 2 cut(s) 47, 88
BglII AGATCT 1 cut(s) 359
BisI GCNGC 3 cut(s) 329, 332, 381
BlsI GCNGC 3 cut(s) 330, 333, 382
Bme1390I CCNGG 2 cut(s) 47, 88
Bme18I GGWCC 1 cut(s) 350
BmgT120I GGNCC 1 cut(s) 350
BmiI GGNNCC 1 cut(s) 60
BmrFI CCNGG 2 cut(s) 47, 88
BmsI GCATC 2 cut(s) 13, 401
Bsa29I ATCGAT 1 cut(s) 269
BsaBI GATNNNNATC 1 cut(s) 39
BsaWI WCCGGW 2 cut(s) 21, 143
Bsc4I CCNNNNNNNGG 3 cut(s) 21, 52, 150
Bse118I RCCGGY 1 cut(s) 324
Bse8I GATNNNNATC 1 cut(s) 39
BseAI TCCGGA 1 cut(s) 21
BseBI CCWGG 2 cut(s) 47, 88
BseCI ATCGAT 1 cut(s) 269
BseGI GGATG 3 cut(s) 4, 159, 496
BseJI GATNNNNATC 1 cut(s) 39
BseLI CCNNNNNNNGG 3 cut(s) 21, 52, 150
BseMII CTCAG 1 cut(s) 387
BseRI GAGGAG 3 cut(s) 65, 68, 197
BseSI GKGCMC 1 cut(s) 261
BseXI GCAGC 1 cut(s) 392
BshFI GGCC 1 cut(s) 293
BshVI ATCGAT 1 cut(s) 269
BsiHKAI GWGCWC 1 cut(s) 261
BsiSI CCGG 3 cut(s) 22, 144, 325
BslI CCNNNNNNNGG 3 cut(s) 21, 52, 150
BsnI GGCC 1 cut(s) 293
Bsp1286I GDGCHC 1 cut(s) 261
Bsp13I TCCGGA 1 cut(s) 21
Bsp143I GATC 9 cut(s) 18, 66, 76, 94, 224, 282, 359, 482, 511
BspACI CCGC 6 cut(s) 234, 328, 331, 334, 348, 480
BspANI GGCC 1 cut(s) 293
BspCNI CTCAG 1 cut(s) 388
BspDI ATCGAT 1 cut(s) 269
BspEI TCCGGA 1 cut(s) 21
BspLI GGNNCC 1 cut(s) 60
BspPI GGATC 5 cut(s) 13, 84, 232, 490, 519
BspQI GCTCTTC 1 cut(s) 360
BsrFI RCCGGY 1 cut(s) 324
BssAI RCCGGY 1 cut(s) 324
BssMI GATC 9 cut(s) 18, 66, 76, 94, 224, 282, 359, 482, 511
Bst2UI CCWGG 2 cut(s) 47, 88
Bst6I CTCTTC 1 cut(s) 360
BstC8I GCNNGC 2 cut(s) 291, 295
BstDEI CTNAG 1 cut(s) 396
BstF5I GGATG 3 cut(s) 4, 159, 496
BstKTI GATC 9 cut(s) 21, 69, 79, 97, 227, 285, 362, 485, 514
BstMBI GATC 9 cut(s) 18, 66, 76, 94, 224, 282, 359, 482, 511
BstMWI GCNNNNNNNGC 1 cut(s) 389
BstNI CCWGG 2 cut(s) 47, 88
BstNSI RCATGY 1 cut(s) 409
BstSCI CCNGG 2 cut(s) 45, 86
BstSLI GKGCMC 1 cut(s) 261
BstV1I GCAGC 1 cut(s) 392
BstX2I RGATCY 2 cut(s) 18, 359
BstYI RGATCY 2 cut(s) 18, 359
Bsu15I ATCGAT 1 cut(s) 269
BsuRI GGCC 1 cut(s) 293
BsuTUI ATCGAT 1 cut(s) 269
BtsCI GGATG 3 cut(s) 4, 159, 496
BtsIMutI CAGTG 1 cut(s) 30
Cac8I GCNNGC 2 cut(s) 291, 295
Cfr10I RCCGGY 1 cut(s) 324
Cfr13I GGNCC 1 cut(s) 350
ClaI ATCGAT 1 cut(s) 269
Csp6I GTAC 3 cut(s) 187, 409, 475
CviAII CATG 3 cut(s) 406, 515, 535
CviJI RGCY 7 cut(s) 207, 239, 289, 293, 297, 370, 383
CviKI_1 RGCY 7 cut(s) 207, 239, 289, 293, 297, 370, 383
CviQI GTAC 3 cut(s) 187, 409, 475
DdeI CTNAG 1 cut(s) 396
DpnI GATC 9 cut(s) 20, 68, 78, 96, 226, 284, 361, 484, 513
DpnII GATC 9 cut(s) 18, 66, 76, 94, 224, 282, 359, 482, 511
EaeI YGGCCR 1 cut(s) 291
Eam1104I CTCTTC 1 cut(s) 360
EarI CTCTTC 1 cut(s) 360
EciI GGCGGA 2 cut(s) 249, 363
Eco47I GGWCC 1 cut(s) 350
Eco57I CTGAAG 2 cut(s) 369, 384
EcoRII CCWGG 2 cut(s) 45, 86
EcoT22I ATGCAT 1 cut(s) 6
FaeI CATG 3 cut(s) 409, 518, 538
FatI CATG 3 cut(s) 405, 514, 534
FauI CCCGC 1 cut(s) 327
Fnu4HI GCNGC 3 cut(s) 329, 332, 381
FokI GGATG 2 cut(s) 166, 503
Fsp4HI GCNGC 3 cut(s) 329, 332, 381
GluI GCNGC 3 cut(s) 329, 332, 381
HaeIII GGCC 1 cut(s) 293
HapII CCGG 3 cut(s) 22, 144, 325
Hin1II CATG 3 cut(s) 409, 518, 538
HinfI GANTC 2 cut(s) 83, 104
HpaII CCGG 3 cut(s) 22, 144, 325
HphI GGTGA 1 cut(s) 35
Hpy166II GTNNAC 1 cut(s) 259
Hpy188I TCNGA 6 cut(s) 94, 118, 364, 388, 397, 456
Hpy188III TCNNGA 4 cut(s) 22, 80, 202, 276
Hpy8I GTNNAC 1 cut(s) 259
Hpy99I CGWCG 2 cut(s) 74, 232
HpyAV CCTTC 2 cut(s) 238, 302
HpyCH4V TGCA 2 cut(s) 4, 259
HpyF10VI GCNNNNNNNGC 1 cut(s) 389
HpyF3I CTNAG 1 cut(s) 396
Hsp92II CATG 3 cut(s) 409, 518, 538
Kpn2I TCCGGA 1 cut(s) 21
Kzo9I GATC 9 cut(s) 18, 66, 76, 94, 224, 282, 359, 482, 511
LguI GCTCTTC 1 cut(s) 360
LmnI GCTCC 1 cut(s) 236
Lsp1109I GCAGC 1 cut(s) 392
LweI GCATC 2 cut(s) 13, 401
MalI GATC 9 cut(s) 20, 68, 78, 96, 226, 284, 361, 484, 513
MboI GATC 9 cut(s) 18, 66, 76, 94, 224, 282, 359, 482, 511
MboII GAAGA 3 cut(s) 326, 377, 431
MflI RGATCY 2 cut(s) 18, 359
MhlI GDGCHC 1 cut(s) 261
MlsI TGGCCA 1 cut(s) 293
MluCI AATT 3 cut(s) 109, 148, 539
MluNI TGGCCA 1 cut(s) 293
MnlI CCTC 8 cut(s) 43, 46, 49, 67, 124, 175, 247, 337
Mox20I TGGCCA 1 cut(s) 293
Mph1103I ATGCAT 1 cut(s) 6
MroI TCCGGA 1 cut(s) 21
MscI TGGCCA 1 cut(s) 293
MseI TTAA 1 cut(s) 522
Msp20I TGGCCA 1 cut(s) 293
MspA1I CMGCKG 1 cut(s) 480
MspI CCGG 3 cut(s) 22, 144, 325
MspR9I CCNGG 2 cut(s) 47, 88
MvaI CCWGG 2 cut(s) 47, 88
MwoI GCNNNNNNNGC 1 cut(s) 389
NdeII GATC 9 cut(s) 18, 66, 76, 94, 224, 282, 359, 482, 511
NlaIII CATG 3 cut(s) 409, 518, 538
NlaIV GGNNCC 1 cut(s) 60
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 1 cut(s) 409
PciSI GCTCTTC 1 cut(s) 360
PfeI GAWTC 2 cut(s) 83, 104
PkrI GCNGC 3 cut(s) 330, 333, 382
PsiI TTATAA 1 cut(s) 562
Psp6I CCWGG 2 cut(s) 45, 86
PspGI CCWGG 2 cut(s) 45, 86
PspN4I GGNNCC 1 cut(s) 60
PspPI GGNCC 1 cut(s) 350
PsuI RGATCY 2 cut(s) 18, 359
RsaI GTAC 3 cut(s) 188, 410, 476
RsaNI GTAC 3 cut(s) 187, 409, 475
SapI GCTCTTC 1 cut(s) 360
SaqAI TTAA 1 cut(s) 522
SatI GCNGC 3 cut(s) 329, 332, 381
Sau3AI GATC 9 cut(s) 18, 66, 76, 94, 224, 282, 359, 482, 511
Sau96I GGNCC 1 cut(s) 350
ScrFI CCNGG 2 cut(s) 47, 88
SduI GDGCHC 1 cut(s) 261
SetI ASST 8 cut(s) 60, 209, 241, 249, 258, 299, 372, 385
SfaNI GCATC 2 cut(s) 13, 401
SgrAI CRCCGGYG 1 cut(s) 324
SinI GGWCC 1 cut(s) 350
Sse9I AATT 3 cut(s) 109, 148, 539
SsiI CCGC 6 cut(s) 234, 328, 331, 334, 348, 480
SspI AATATT 1 cut(s) 460
StyD4I CCNGG 2 cut(s) 45, 86
TaqI TCGA 3 cut(s) 72, 227, 269
TasI AATT 3 cut(s) 109, 148, 539
TatI WGTACW 1 cut(s) 408
TauI GCSGC 2 cut(s) 331, 334
TfiI GAWTC 2 cut(s) 83, 104
Tru1I TTAA 1 cut(s) 522
Tru9I TTAA 1 cut(s) 522
TscAI CASTG 1 cut(s) 37
TseI GCWGC 1 cut(s) 380
TspDTI ATGAA 2 cut(s) 321, 551
TspRI CASTG 1 cut(s) 37
VneI GTGCAC 1 cut(s) 257
VpaK11BI GGWCC 1 cut(s) 350
XapI RAATTY 1 cut(s) 539
XceI RCATGY 1 cut(s) 409
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.