Rh2DG358600

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0018229
Physical Location & Seq
Forward (+)
50493237 .. 50498284
5048 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG358600.1

Sequence Viewer

Length: 387 bp
ATGAAGATTCGGAGTGTGATTGATGTGAGTGAGGAGTACGAGAGTATTGTCAAGAGCTTTGTTGTGGGTATGGGATCGGCGAACGGAGTTACGGAGGTTGTGGCGGTGCACAAGAAATTGATTTATGGACTGACCGCGCTGTACTTGAATATTAATAGCCTGAAAAATGAAGGAGAACAACGACACCGGCGGCGACAGCGGGTATGTGAGGACGGACCAGATAGATCTGAAGAGCTTGGACGAGCAGCTTCAGAAGCATCTGAGCAGGGCATGGACTATGGAGAAGAACAAGAACAGAGACCAGCAGGAACAACAAATCTGAATATTAGCGTCGGAGGCATGTCACCTGGTGGTCCGAAGAAAAAGAAATATGGAGATGTCAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

128

Amino Acids

14.04

Weight (kDa)

6.75

Isoelectric Point (pI)

61.92

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 137
AciI CCGC 4 cut(s) 104, 135, 190, 199
AclWI GGATC 1 cut(s) 82
AcuI CTGAAG 2 cut(s) 234, 249
AdeI CACNNNGTG 1 cut(s) 350
AfaI GTAC 2 cut(s) 38, 143
AgsI TTSAA 1 cut(s) 148
AjnI CCWGG 1 cut(s) 346
AluBI AGCT 3 cut(s) 57, 235, 248
AluI AGCT 3 cut(s) 57, 235, 248
Alw21I GWGCWC 1 cut(s) 111
Alw26I GTCTC 1 cut(s) 292
Alw44I GTGCAC 1 cut(s) 107
AlwI GGATC 1 cut(s) 82
ApaLI GTGCAC 1 cut(s) 107
ApeKI GCWGC 1 cut(s) 245
AseI ATTAAT 1 cut(s) 153
AspLEI GCGC 1 cut(s) 139
AspS9I GGNCC 2 cut(s) 215, 353
AsuHPI GGTGA 1 cut(s) 336
AvaII GGWCC 2 cut(s) 215, 353
BaeGI GKGCMC 1 cut(s) 111
Bbv12I GWGCWC 1 cut(s) 111
BbvI GCAGC 1 cut(s) 257
BciT130I CCWGG 1 cut(s) 348
BcoDI GTCTC 1 cut(s) 292
BglII AGATCT 1 cut(s) 224
BisI GCNGC 2 cut(s) 191, 246
BlsI GCNGC 2 cut(s) 192, 247
Bme1390I CCNGG 1 cut(s) 348
Bme18I GGWCC 2 cut(s) 215, 353
BmgT120I GGNCC 2 cut(s) 215, 353
BmrFI CCNGG 1 cut(s) 348
BmsI GCATC 1 cut(s) 266
BsaI GGTCTC 1 cut(s) 292
Bse118I RCCGGY 1 cut(s) 186
BseBI CCWGG 1 cut(s) 348
BseMII CTCAG 1 cut(s) 252
BseRI GAGGAG 1 cut(s) 47
BseSI GKGCMC 1 cut(s) 111
BseXI GCAGC 1 cut(s) 257
Bsh1236I CGCG 1 cut(s) 137
BsiHKAI GWGCWC 1 cut(s) 111
BsiSI CCGG 1 cut(s) 187
BsmAI GTCTC 1 cut(s) 292
Bso31I GGTCTC 1 cut(s) 292
Bsp1286I GDGCHC 1 cut(s) 111
Bsp143I GATC 2 cut(s) 74, 224
BspACI CCGC 4 cut(s) 104, 135, 190, 199
BspCNI CTCAG 1 cut(s) 253
BspFNI CGCG 1 cut(s) 137
BspPI GGATC 1 cut(s) 82
BspQI GCTCTTC 1 cut(s) 225
BspTNI GGTCTC 1 cut(s) 292
BsrFI RCCGGY 1 cut(s) 186
BssAI RCCGGY 1 cut(s) 186
BssMI GATC 2 cut(s) 74, 224
Bst2UI CCWGG 1 cut(s) 348
Bst6I CTCTTC 1 cut(s) 225
BstDEI CTNAG 1 cut(s) 261
BstFNI CGCG 1 cut(s) 137
BstHHI GCGC 1 cut(s) 139
BstKTI GATC 2 cut(s) 77, 227
BstMAI GTCTC 1 cut(s) 292
BstMBI GATC 2 cut(s) 74, 224
BstMWI GCNNNNNNNGC 3 cut(s) 196, 254, 336
BstNI CCWGG 1 cut(s) 348
BstNSI RCATGY 1 cut(s) 343
BstSCI CCNGG 1 cut(s) 346
BstSLI GKGCMC 1 cut(s) 111
BstUI CGCG 1 cut(s) 137
BstV1I GCAGC 1 cut(s) 257
BstX2I RGATCY 1 cut(s) 224
BstYI RGATCY 1 cut(s) 224
CfoI GCGC 1 cut(s) 139
Cfr10I RCCGGY 1 cut(s) 186
Cfr13I GGNCC 2 cut(s) 215, 353
CseI GACGC 1 cut(s) 319
CsiI ACCWGGT 1 cut(s) 346
Csp6I GTAC 2 cut(s) 37, 142
CviAII CATG 2 cut(s) 271, 340
CviJI RGCY 4 cut(s) 57, 159, 235, 248
CviKI_1 RGCY 4 cut(s) 57, 159, 235, 248
CviQI GTAC 2 cut(s) 37, 142
DdeI CTNAG 1 cut(s) 261
DpnI GATC 2 cut(s) 76, 226
DpnII GATC 2 cut(s) 74, 224
DraIII CACNNNGTG 1 cut(s) 350
Eam1104I CTCTTC 1 cut(s) 225
EarI CTCTTC 1 cut(s) 225
Eco31I GGTCTC 1 cut(s) 292
Eco47I GGWCC 2 cut(s) 215, 353
Eco57I CTGAAG 2 cut(s) 234, 249
EcoRII CCWGG 1 cut(s) 346
FaeI CATG 2 cut(s) 274, 343
FaiI YATR 7 cut(s) 71, 126, 205, 272, 279, 341, 372
FatI CATG 2 cut(s) 270, 339
FauI CCCGC 1 cut(s) 192
Fnu4HI GCNGC 2 cut(s) 191, 246
Fsp4HI GCNGC 2 cut(s) 191, 246
GlaI GCGC 1 cut(s) 138
GluI GCNGC 2 cut(s) 191, 246
HapII CCGG 1 cut(s) 187
HgaI GACGC 1 cut(s) 319
HhaI GCGC 1 cut(s) 139
Hin1II CATG 2 cut(s) 274, 343
Hin6I GCGC 1 cut(s) 137
HinP1I GCGC 1 cut(s) 137
HinfI GANTC 1 cut(s) 7
HpaII CCGG 1 cut(s) 187
HphI GGTGA 1 cut(s) 336
Hpy166II GTNNAC 1 cut(s) 109
Hpy188I TCNGA 7 cut(s) 12, 229, 253, 262, 321, 335, 357
Hpy188III TCNNGA 1 cut(s) 52
Hpy8I GTNNAC 1 cut(s) 109
Hpy99I CGWCG 1 cut(s) 335
HpyAV CCTTC 1 cut(s) 164
HpyCH4V TGCA 1 cut(s) 109
HpyF10VI GCNNNNNNNGC 3 cut(s) 196, 254, 336
HpyF3I CTNAG 1 cut(s) 261
Hsp92II CATG 2 cut(s) 274, 343
HspAI GCGC 1 cut(s) 137
Kzo9I GATC 2 cut(s) 74, 224
LguI GCTCTTC 1 cut(s) 225
LpnPI CCDG 8 cut(s) 173, 200, 231, 251, 291, 315, 333, 360
Lsp1109I GCAGC 1 cut(s) 257
LweI GCATC 1 cut(s) 266
MabI ACCWGGT 1 cut(s) 346
MaeIII GTNAC 2 cut(s) 88, 342
MalI GATC 2 cut(s) 76, 226
MboI GATC 2 cut(s) 74, 224
MboII GAAGA 4 cut(s) 16, 242, 296, 370
MflI RGATCY 1 cut(s) 224
MhlI GDGCHC 1 cut(s) 111
MluCI AATT 1 cut(s) 116
MmeI TCCRAC 1 cut(s) 313
MnlI CCTC 4 cut(s) 25, 88, 202, 329
MseI TTAA 1 cut(s) 153
MspA1I CMGCKG 1 cut(s) 199
MspI CCGG 1 cut(s) 187
MspR9I CCNGG 1 cut(s) 348
MvaI CCWGG 1 cut(s) 348
MvnI CGCG 1 cut(s) 137
MwoI GCNNNNNNNGC 3 cut(s) 196, 254, 336
NdeII GATC 2 cut(s) 74, 224
NlaIII CATG 2 cut(s) 274, 343
NmuCI GTSAC 1 cut(s) 342
NspI RCATGY 1 cut(s) 343
PciSI GCTCTTC 1 cut(s) 225
PfeI GAWTC 1 cut(s) 7
PkrI GCNGC 2 cut(s) 192, 247
PshBI ATTAAT 1 cut(s) 153
Psp6I CCWGG 1 cut(s) 346
PspGI CCWGG 1 cut(s) 346
PspPI GGNCC 2 cut(s) 215, 353
PsuI RGATCY 1 cut(s) 224
RsaI GTAC 2 cut(s) 38, 143
RsaNI GTAC 2 cut(s) 37, 142
SapI GCTCTTC 1 cut(s) 225
SaqAI TTAA 1 cut(s) 153
SatI GCNGC 2 cut(s) 191, 246
Sau3AI GATC 2 cut(s) 74, 224
Sau96I GGNCC 2 cut(s) 215, 353
ScrFI CCNGG 1 cut(s) 348
SduI GDGCHC 1 cut(s) 111
SetI ASST 5 cut(s) 59, 99, 237, 250, 349
SexAI ACCWGGT 1 cut(s) 346
SfaNI GCATC 1 cut(s) 266
SgrAI CRCCGGYG 1 cut(s) 186
SinI GGWCC 2 cut(s) 215, 353
Sse9I AATT 1 cut(s) 116
SsiI CCGC 4 cut(s) 104, 135, 190, 199
SspI AATATT 2 cut(s) 151, 325
StyD4I CCNGG 1 cut(s) 346
TasI AATT 1 cut(s) 116
TatI WGTACW 1 cut(s) 141
TauI GCSGC 1 cut(s) 193
TfiI GAWTC 1 cut(s) 7
Tru1I TTAA 1 cut(s) 153
Tru9I TTAA 1 cut(s) 153
TseFI GTSAC 1 cut(s) 342
TseI GCWGC 1 cut(s) 245
Tsp45I GTSAC 1 cut(s) 342
TspDTI ATGAA 2 cut(s) 17, 183
TspGWI ACGGA 3 cut(s) 99, 107, 228
VneI GTGCAC 1 cut(s) 107
VpaK11BI GGWCC 2 cut(s) 215, 353
VspI ATTAAT 1 cut(s) 153
XceI RCATGY 1 cut(s) 343
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.