Rmu_sc0008421.1_g000003

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0008421.1
OG0018685
Physical Location & Seq
Forward (+)
19097 .. 21101
2005 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0008421.1_g000003.1.cds

Sequence Viewer

Length: 489 bp
atgggtgcagcggatcagggttccaactgtgagcttggaagggagggtcatggagattgcgacggcggcgacgatcacggccatggtgaagtagagtatggcgtgctagcaggtgcattaggaaagaggaagtcggtggagctcgctgtcggttggatcgtcggagtcggcaggagtaatcgagtggtgttgtcgtttagaaatgaaggagatctaaagtgggcaatgaaaggctacccatggtcctttgagaaagccttggtagcggttgcggtagcagatggtcgtgaggatccaaagaaggcaggttcgctggaaacccggagatttgaactggagtatgagtgtcttccacatttttacttcttttgtagtctactgtcacacataggggcgaagtgctcatgcaaggagaagggagaattgactgaacctgcttatgacgcttttatgaatgcggagagaaaagtggcttggttgcttgagtag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

162

Amino Acids

17.76

Weight (kDa)

5.43

Isoelectric Point (pI)

27.62

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 101
Acc36I ACCTGC 3 cut(s) 101, 296, 442
AccI GTMKAC 1 cut(s) 376
AciI CCGC 5 cut(s) 11, 66, 266, 272, 458
AclWI GGATC 4 cut(s) 21, 164, 287, 300
AcoI YGGCCR 1 cut(s) 79
AgsI TTSAA 1 cut(s) 332
AjuI GAANNNNNNNTTGG 2 cut(s) 457, 489
AluBI AGCT 2 cut(s) 34, 142
AluI AGCT 2 cut(s) 34, 142
Alw21I GWGCWC 2 cut(s) 144, 404
AlwI GGATC 4 cut(s) 21, 164, 287, 300
AoxI GGCC 1 cut(s) 79
ApeKI GCWGC 1 cut(s) 8
AspS9I GGNCC 1 cut(s) 243
AsuC2I CCSGG 1 cut(s) 322
AsuHPI GGTGA 1 cut(s) 98
AsuNHI GCTAGC 1 cut(s) 106
AvaII GGWCC 1 cut(s) 243
BamHI GGATCC 1 cut(s) 292
BanII GRGCYC 1 cut(s) 144
BbsI GAAGAC 1 cut(s) 341
Bbv12I GWGCWC 2 cut(s) 144, 404
BbvI GCAGC 1 cut(s) 20
BccI CCATC 1 cut(s) 275
BceAI ACGGC 2 cut(s) 79, 94
BcnI CCSGG 1 cut(s) 322
BfaI CTAG 1 cut(s) 107
BfuAI ACCTGC 3 cut(s) 101, 296, 442
BglII AGATCT 1 cut(s) 211
BisI GCNGC 2 cut(s) 9, 67
BlsI GCNGC 2 cut(s) 10, 68
Bme1390I CCNGG 1 cut(s) 322
Bme18I GGWCC 1 cut(s) 243
BmgT120I GGNCC 1 cut(s) 243
BmiI GGNNCC 2 cut(s) 22, 294
BmrFI CCNGG 1 cut(s) 322
BmtI GCTAGC 1 cut(s) 110
BpiI GAAGAC 1 cut(s) 341
BpmI CTGGAG 1 cut(s) 356
BpuMI CCSGG 1 cut(s) 322
BsaJI CCNNGG 3 cut(s) 82, 239, 258
BsaXI ACNNNNNCTCC 2 cut(s) 329, 359
Bse1I ACTGG 1 cut(s) 339
Bse3DI GCAATG 1 cut(s) 231
BseDI CCNNGG 3 cut(s) 82, 239, 258
BseMI GCAATG 1 cut(s) 231
BseNI ACTGG 1 cut(s) 339
BseXI GCAGC 1 cut(s) 20
BsgI GTGCAG 1 cut(s) 27
BshFI GGCC 1 cut(s) 81
BsiHKAI GWGCWC 2 cut(s) 144, 404
BsiSI CCGG 1 cut(s) 322
BsmI GAATGC 1 cut(s) 460
BsnI GGCC 1 cut(s) 81
Bsp1286I GDGCHC 2 cut(s) 144, 404
Bsp143I GATC 5 cut(s) 13, 73, 156, 211, 292
Bsp19I CCATGG 2 cut(s) 82, 239
BspACI CCGC 5 cut(s) 11, 66, 266, 272, 458
BspANI GGCC 1 cut(s) 81
BspLI GGNNCC 2 cut(s) 22, 294
BspMI ACCTGC 3 cut(s) 101, 296, 442
BspOI GCTAGC 1 cut(s) 110
BspPI GGATC 4 cut(s) 21, 164, 287, 300
BsrDI GCAATG 1 cut(s) 231
BsrI ACTGG 1 cut(s) 339
BssECI CCNNGG 3 cut(s) 82, 239, 258
BssMI GATC 5 cut(s) 13, 73, 156, 211, 292
BssT1I CCWWGG 3 cut(s) 82, 239, 258
Bst4CI ACNGT 2 cut(s) 29, 381
BstC8I GCNNGC 3 cut(s) 104, 108, 144
BstDSI CCRYGG 2 cut(s) 82, 239
BstKTI GATC 5 cut(s) 16, 76, 159, 214, 295
BstMBI GATC 5 cut(s) 13, 73, 156, 211, 292
BstMWI GCNNNNNNNGC 3 cut(s) 66, 263, 443
BstSCI CCNGG 1 cut(s) 320
BstV1I GCAGC 1 cut(s) 20
BstV2I GAAGAC 1 cut(s) 341
BstX2I RGATCY 2 cut(s) 211, 292
BstYI RGATCY 2 cut(s) 211, 292
BsuRI GGCC 1 cut(s) 81
BtgI CCRYGG 2 cut(s) 82, 239
BveI ACCTGC 3 cut(s) 101, 296, 442
Cac8I GCNNGC 3 cut(s) 104, 108, 144
Cfr13I GGNCC 1 cut(s) 243
CseI GACGC 1 cut(s) 452
CviAII CATG 4 cut(s) 50, 83, 240, 405
CviJI RGCY 6 cut(s) 34, 81, 142, 234, 257, 473
CviKI_1 RGCY 6 cut(s) 34, 81, 142, 234, 257, 473
DpnI GATC 5 cut(s) 15, 75, 158, 213, 294
DpnII GATC 5 cut(s) 13, 73, 156, 211, 292
EaeI YGGCCR 1 cut(s) 79
Ecl136II GAGCTC 1 cut(s) 142
Eco130I CCWWGG 3 cut(s) 82, 239, 258
Eco24I GRGCYC 1 cut(s) 144
Eco47I GGWCC 1 cut(s) 243
Eco53kI GAGCTC 1 cut(s) 142
EcoICRI GAGCTC 1 cut(s) 142
EcoT14I CCWWGG 3 cut(s) 82, 239, 258
EcoT38I GRGCYC 1 cut(s) 144
ErhI CCWWGG 3 cut(s) 82, 239, 258
FaeI CATG 4 cut(s) 53, 86, 243, 408
FaiI YATR 9 cut(s) 51, 84, 99, 241, 342, 389, 406, 441, 452
FatI CATG 4 cut(s) 49, 82, 239, 404
FblI GTMKAC 1 cut(s) 376
Fnu4HI GCNGC 2 cut(s) 9, 67
FriOI GRGCYC 1 cut(s) 144
Fsp4HI GCNGC 2 cut(s) 9, 67
FspBI CTAG 1 cut(s) 107
GluI GCNGC 2 cut(s) 9, 67
GsuI CTGGAG 1 cut(s) 356
HaeIII GGCC 1 cut(s) 81
HapII CCGG 1 cut(s) 322
HgaI GACGC 1 cut(s) 452
Hin1II CATG 4 cut(s) 53, 86, 243, 408
HinfI GANTC 1 cut(s) 165
HpaII CCGG 1 cut(s) 322
HphI GGTGA 1 cut(s) 98
Hpy166II GTNNAC 1 cut(s) 377
Hpy188I TCNGA 1 cut(s) 164
Hpy188III TCNNGA 1 cut(s) 287
Hpy8I GTNNAC 1 cut(s) 377
Hpy99I CGWCG 3 cut(s) 65, 74, 164
HpyAV CCTTC 4 cut(s) 33, 200, 295, 409
HpyCH4III ACNGT 2 cut(s) 29, 381
HpyCH4V TGCA 3 cut(s) 8, 116, 408
HpyF10VI GCNNNNNNNGC 3 cut(s) 66, 263, 443
Hsp92II CATG 4 cut(s) 53, 86, 243, 408
Kzo9I GATC 5 cut(s) 13, 73, 156, 211, 292
LmnI GCTCC 1 cut(s) 139
LpnPI CCDG 8 cut(s) 2, 96, 157, 291, 299, 320, 335, 447
Lsp1109I GCAGC 1 cut(s) 20
MaeI CTAG 1 cut(s) 107
MaeIII GTNAC 1 cut(s) 381
MalI GATC 5 cut(s) 15, 75, 158, 213, 294
MboI GATC 5 cut(s) 13, 73, 156, 211, 292
MboII GAAGA 1 cut(s) 341
MflI RGATCY 2 cut(s) 211, 292
MhlI GDGCHC 2 cut(s) 144, 404
MluCI AATT 1 cut(s) 422
MlyI GAGTC 1 cut(s) 174
MmeI TCCRAC 3 cut(s) 48, 134, 142
MnlI CCTC 3 cut(s) 37, 120, 283
MslI CAYNNNNRTG 1 cut(s) 81
MspA1I CMGCKG 1 cut(s) 11
MspI CCGG 1 cut(s) 322
MspR9I CCNGG 1 cut(s) 322
Mva1269I GAATGC 1 cut(s) 460
MwoI GCNNNNNNNGC 3 cut(s) 66, 263, 443
NciI CCSGG 1 cut(s) 322
NcoI CCATGG 2 cut(s) 82, 239
NdeII GATC 5 cut(s) 13, 73, 156, 211, 292
NheI GCTAGC 1 cut(s) 106
NlaIII CATG 4 cut(s) 53, 86, 243, 408
NlaIV GGNNCC 2 cut(s) 22, 294
NmuCI GTSAC 1 cut(s) 381
PaqCI CACCTGC 1 cut(s) 101
PcsI WCGNNNNNNNCGW 2 cut(s) 69, 156
PctI GAATGC 1 cut(s) 460
PkrI GCNGC 2 cut(s) 10, 68
PleI GAGTC 1 cut(s) 173
PpsI GAGTC 1 cut(s) 173
Psp124BI GAGCTC 1 cut(s) 144
PspN4I GGNNCC 2 cut(s) 22, 294
PspPI GGNCC 1 cut(s) 243
PsuI RGATCY 2 cut(s) 211, 292
RseI CAYNNNNRTG 1 cut(s) 81
SacI GAGCTC 1 cut(s) 144
SatI GCNGC 2 cut(s) 9, 67
Sau3AI GATC 5 cut(s) 13, 73, 156, 211, 292
Sau96I GGNCC 1 cut(s) 243
SchI GAGTC 1 cut(s) 174
ScrFI CCNGG 1 cut(s) 322
SduI GDGCHC 2 cut(s) 144, 404
SetI ASST 5 cut(s) 36, 115, 144, 310, 436
SinI GGWCC 1 cut(s) 243
SmiMI CAYNNNNRTG 1 cut(s) 81
SmlI CTYRAG 1 cut(s) 482
SmoI CTYRAG 1 cut(s) 482
Sse9I AATT 1 cut(s) 422
SsiI CCGC 5 cut(s) 11, 66, 266, 272, 458
SspMI CTAG 1 cut(s) 107
SstI GAGCTC 1 cut(s) 144
StyD4I CCNGG 1 cut(s) 320
StyI CCWWGG 3 cut(s) 82, 239, 258
TaaI ACNGT 2 cut(s) 29, 381
TaqI TCGA 1 cut(s) 181
TasI AATT 1 cut(s) 422
TauI GCSGC 1 cut(s) 69
TseFI GTSAC 1 cut(s) 381
TseI GCWGC 1 cut(s) 8
Tsp45I GTSAC 1 cut(s) 381
TspDTI ATGAA 3 cut(s) 219, 242, 467
VpaK11BI GGWCC 1 cut(s) 243
XmiI GTMKAC 1 cut(s) 376
XspI CTAG 1 cut(s) 107
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.