Rroxscaffold_6G00399990

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0018685
Physical Location & Seq
Forward (+)
22045495 .. 22046091
597 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00399990.1

Sequence Viewer

Length: 507 bp
ATGTGCACACAAGTAAAGATCGCTTTGGCGGTTGCAGGGAGTTACCTCAAGATTAGAGTGGGTGTTGACATCTCGGTGCCCACGAGAAAATGGATCGGTTTTGAGGGCGGGGGAGAGGCCAACCTTCGTGATGTTGAATATGAACGATTGCCGCATTTGTTTCTATTGTGGGCTCCTCTCCCACACGAGCACAAACTGCCTGCAAAGGAGTCGGATGAGATATTGGAGCCGGCGTATGATGCTCTATTGAATGCGGAGAAGAAGGAGGCATGGTTTGCGAAGCAAGAGCGAGGTACGAACTCACCGAGAAATGGTGTCGGACGGTGGGCTGAGATGGAAGAAGAGGAGGCTCCAATGGTGGCTGGGGTTGATCAGATAGAGGTCGATAGAGCAGCTGGTTTGGAGCGGATACGGGGGGATGCGAAACTGCGGTGTGTAGGTGACATAGCGATGTACTGCGACGGCCATTGCCGGATCCGACGTCGGACGCGAGTTGACTCTGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

168

Amino Acids

18.9

Weight (kDa)

5.87

Isoelectric Point (pI)

44.97

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 484
AccB1I GGYRCC 1 cut(s) 76
AccBSI CCGCTC 1 cut(s) 406
AccII CGCG 1 cut(s) 490
AciI CCGC 6 cut(s) 29, 108, 152, 254, 406, 430
AclWI GGATC 3 cut(s) 101, 469, 482
AcoI YGGCCR 1 cut(s) 463
AcyI GRCGYC 1 cut(s) 481
AfaI GTAC 2 cut(s) 295, 455
AfiI CCNNNNNNNGG 1 cut(s) 311
AgsI TTSAA 2 cut(s) 137, 250
AluBI AGCT 1 cut(s) 395
AluI AGCT 1 cut(s) 395
Alw21I GWGCWC 2 cut(s) 8, 192
Alw44I GTGCAC 1 cut(s) 4
AlwI GGATC 3 cut(s) 101, 469, 482
AoxI GGCC 2 cut(s) 117, 463
ApaLI GTGCAC 1 cut(s) 4
ApeKI GCWGC 1 cut(s) 392
AsuHPI GGTGA 2 cut(s) 294, 452
BaeGI GKGCMC 2 cut(s) 8, 81
BamHI GGATCC 1 cut(s) 474
BanI GGYRCC 1 cut(s) 76
BanII GRGCYC 1 cut(s) 175
BauI CACGAG 2 cut(s) 82, 185
Bbv12I GWGCWC 2 cut(s) 8, 192
BbvI GCAGC 1 cut(s) 404
BccI CCATC 1 cut(s) 328
BceAI ACGGC 1 cut(s) 478
BcgI CGANNNNNNTGC 2 cut(s) 192, 226
BciVI GTATCC 1 cut(s) 402
BclI TGATCA 1 cut(s) 370
BfuI GTATCC 1 cut(s) 402
BisI GCNGC 2 cut(s) 152, 393
BlsI GCNGC 2 cut(s) 153, 394
BmiI GGNNCC 5 cut(s) 78, 174, 228, 351, 476
BmsI GCATC 2 cut(s) 229, 409
BpuEI CTTGAG 1 cut(s) 32
BsaHI GRCGYC 1 cut(s) 481
BsaXI ACNNNNNCTCC 4 cut(s) 218, 248, 257, 287
Bsc4I CCNNNNNNNGG 1 cut(s) 311
Bse118I RCCGGY 1 cut(s) 229
Bse3DI GCAATG 1 cut(s) 466
BseGI GGATG 2 cut(s) 220, 424
BseLI CCNNNNNNNGG 1 cut(s) 311
BseMI GCAATG 1 cut(s) 466
BseMII CTCAG 1 cut(s) 321
BseRI GAGGAG 2 cut(s) 165, 359
BseSI GKGCMC 2 cut(s) 8, 81
BseXI GCAGC 1 cut(s) 404
BseYI CCCAGC 1 cut(s) 362
Bsh1236I CGCG 1 cut(s) 490
BshFI GGCC 2 cut(s) 119, 465
BshNI GGYRCC 1 cut(s) 76
BsiHKAI GWGCWC 2 cut(s) 8, 192
BsiSI CCGG 2 cut(s) 230, 472
BslI CCNNNNNNNGG 1 cut(s) 311
BsmI GAATGC 1 cut(s) 256
BsnI GGCC 2 cut(s) 119, 465
Bsp1286I GDGCHC 4 cut(s) 8, 81, 175, 192
Bsp143I GATC 4 cut(s) 18, 93, 370, 474
BspACI CCGC 6 cut(s) 29, 108, 152, 254, 406, 430
BspANI GGCC 2 cut(s) 119, 465
BspCNI CTCAG 1 cut(s) 322
BspFNI CGCG 1 cut(s) 490
BspLI GGNNCC 5 cut(s) 78, 174, 228, 351, 476
BspPI GGATC 3 cut(s) 101, 469, 482
BspT107I GGYRCC 1 cut(s) 76
BsrBI CCGCTC 1 cut(s) 406
BsrDI GCAATG 1 cut(s) 466
BsrFI RCCGGY 1 cut(s) 229
BssAI RCCGGY 1 cut(s) 229
BssMI GATC 4 cut(s) 18, 93, 370, 474
BssNI GRCGYC 1 cut(s) 481
BssSI CACGAG 2 cut(s) 82, 185
Bst2BI CACGAG 2 cut(s) 82, 185
Bst4CI ACNGT 1 cut(s) 324
Bst6I CTCTTC 1 cut(s) 336
BstACI GRCGYC 1 cut(s) 481
BstAPI GCANNNNNTGC 2 cut(s) 196, 275
BstC8I GCNNGC 2 cut(s) 201, 231
BstDEI CTNAG 1 cut(s) 330
BstF5I GGATG 2 cut(s) 220, 424
BstFNI CGCG 1 cut(s) 490
BstKTI GATC 4 cut(s) 21, 96, 373, 477
BstMBI GATC 4 cut(s) 18, 93, 370, 474
BstMWI GCNNNNNNNGC 3 cut(s) 196, 239, 275
BstSLI GKGCMC 2 cut(s) 8, 81
BstUI CGCG 1 cut(s) 490
BstV1I GCAGC 1 cut(s) 404
BstX2I RGATCY 1 cut(s) 474
BstYI RGATCY 1 cut(s) 474
BsuI GTATCC 1 cut(s) 402
BsuRI GGCC 2 cut(s) 119, 465
BtgZI GCGATG 1 cut(s) 464
BtsCI GGATG 2 cut(s) 220, 424
Cac8I GCNNGC 2 cut(s) 201, 231
Cfr10I RCCGGY 1 cut(s) 229
CseI GACGC 1 cut(s) 496
Csp6I GTAC 2 cut(s) 294, 454
CviAII CATG 1 cut(s) 270
CviJI RGCY 8 cut(s) 119, 173, 229, 329, 350, 362, 395, 465
CviKI_1 RGCY 8 cut(s) 119, 173, 229, 329, 350, 362, 395, 465
CviQI GTAC 2 cut(s) 294, 454
DdeI CTNAG 1 cut(s) 330
DpnI GATC 4 cut(s) 20, 95, 372, 476
DpnII GATC 4 cut(s) 18, 93, 370, 474
EaeI YGGCCR 1 cut(s) 463
Eam1104I CTCTTC 1 cut(s) 336
EarI CTCTTC 1 cut(s) 336
Eco24I GRGCYC 1 cut(s) 175
EcoT38I GRGCYC 1 cut(s) 175
FaeI CATG 1 cut(s) 273
FaiI YATR 4 cut(s) 141, 237, 271, 446
FatI CATG 1 cut(s) 269
FauI CCCGC 1 cut(s) 101
FbaI TGATCA 1 cut(s) 370
Fnu4HI GCNGC 2 cut(s) 152, 393
FokI GGATG 2 cut(s) 227, 431
FriOI GRGCYC 1 cut(s) 175
Fsp4HI GCNGC 2 cut(s) 152, 393
GluI GCNGC 2 cut(s) 152, 393
GsaI CCCAGC 1 cut(s) 366
HaeIII GGCC 2 cut(s) 119, 465
HapII CCGG 2 cut(s) 230, 472
HgaI GACGC 1 cut(s) 496
Hin1I GRCGYC 1 cut(s) 481
Hin1II CATG 1 cut(s) 273
HincII GTYRAC 2 cut(s) 67, 496
HindII GTYRAC 2 cut(s) 67, 496
HinfI GANTC 2 cut(s) 209, 497
HpaII CCGG 2 cut(s) 230, 472
HphI GGTGA 2 cut(s) 294, 452
Hpy166II GTNNAC 3 cut(s) 6, 67, 496
Hpy188I TCNGA 5 cut(s) 214, 320, 375, 479, 486
Hpy188III TCNNGA 2 cut(s) 49, 128
Hpy8I GTNNAC 3 cut(s) 6, 67, 496
Hpy99I CGWCG 3 cut(s) 464, 483, 486
HpyAV CCTTC 2 cut(s) 134, 256
HpyCH4III ACNGT 1 cut(s) 324
HpyCH4IV ACGT 1 cut(s) 481
HpyCH4V TGCA 3 cut(s) 6, 35, 203
HpyF10VI GCNNNNNNNGC 3 cut(s) 196, 239, 275
HpyF3I CTNAG 1 cut(s) 330
HpySE526I ACGT 1 cut(s) 481
Hsp92I GRCGYC 1 cut(s) 481
Hsp92II CATG 1 cut(s) 273
KroI GCCGGC 1 cut(s) 229
KroNI GCCGGC 1 cut(s) 231
Ksp22I TGATCA 1 cut(s) 370
Kzo9I GATC 4 cut(s) 18, 93, 370, 474
LmnI GCTCC 4 cut(s) 178, 226, 355, 403
LpnPI CCDG 6 cut(s) 21, 213, 243, 348, 381, 485
Lsp1109I GCAGC 1 cut(s) 404
LweI GCATC 2 cut(s) 229, 409
MaeII ACGT 1 cut(s) 481
MaeIII GTNAC 2 cut(s) 41, 440
MalI GATC 4 cut(s) 20, 95, 372, 476
MbiI CCGCTC 1 cut(s) 406
MboI GATC 4 cut(s) 18, 93, 370, 474
MboII GAAGA 3 cut(s) 271, 350, 353
MflI RGATCY 1 cut(s) 474
MhlI GDGCHC 4 cut(s) 8, 81, 175, 192
MlyI GAGTC 2 cut(s) 218, 491
MmeI TCCRAC 4 cut(s) 192, 298, 464, 502
MnlI CCTC 9 cut(s) 56, 97, 109, 186, 259, 284, 337, 340, 373
MroNI GCCGGC 1 cut(s) 229
MslI CAYNNNNRTG 2 cut(s) 74, 449
MspA1I CMGCKG 1 cut(s) 395
MspI CCGG 2 cut(s) 230, 472
Mva1269I GAATGC 1 cut(s) 256
MvnI CGCG 1 cut(s) 490
MwoI GCNNNNNNNGC 3 cut(s) 196, 239, 275
NaeI GCCGGC 1 cut(s) 231
NdeII GATC 4 cut(s) 18, 93, 370, 474
NgoMIV GCCGGC 1 cut(s) 229
NlaIII CATG 1 cut(s) 273
NlaIV GGNNCC 5 cut(s) 78, 174, 228, 351, 476
NmuCI GTSAC 1 cut(s) 440
PcsI WCGNNNNNNNCGW 3 cut(s) 80, 302, 487
PctI GAATGC 1 cut(s) 256
PdiI GCCGGC 1 cut(s) 231
PkrI GCNGC 2 cut(s) 153, 394
PleI GAGTC 2 cut(s) 217, 491
PpsI GAGTC 2 cut(s) 217, 491
PspFI CCCAGC 1 cut(s) 362
PspN4I GGNNCC 5 cut(s) 78, 174, 228, 351, 476
PsuI RGATCY 1 cut(s) 474
PvuII CAGCTG 1 cut(s) 395
RsaI GTAC 2 cut(s) 295, 455
RsaNI GTAC 2 cut(s) 294, 454
RseI CAYNNNNRTG 2 cut(s) 74, 449
SatI GCNGC 2 cut(s) 152, 393
Sau3AI GATC 4 cut(s) 18, 93, 370, 474
SchI GAGTC 2 cut(s) 218, 491
SduI GDGCHC 4 cut(s) 8, 81, 175, 192
SetI ASST 7 cut(s) 48, 126, 295, 384, 397, 442, 484
SfaNI GCATC 2 cut(s) 229, 409
SmiMI CAYNNNNRTG 2 cut(s) 74, 449
SmlI CTYRAG 1 cut(s) 47
SmoI CTYRAG 1 cut(s) 47
SsiI CCGC 6 cut(s) 29, 108, 152, 254, 406, 430
TaaI ACNGT 1 cut(s) 324
TaiI ACGT 1 cut(s) 484
TaqI TCGA 1 cut(s) 384
TatI WGTACW 1 cut(s) 453
TauI GCSGC 1 cut(s) 154
TseFI GTSAC 1 cut(s) 440
TseI GCWGC 1 cut(s) 392
Tsp45I GTSAC 1 cut(s) 440
TspDTI ATGAA 1 cut(s) 156
VneI GTGCAC 1 cut(s) 4
ZraI GACGTC 1 cut(s) 482
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.