Rw3G022060

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0018685
Physical Location & Seq
Reverse (-)
26927313 .. 26928254
942 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G022060.1

Sequence Viewer

Length: 648 bp
ATGCGAGGGCTTTGGCTACAGAAGGATGGCCCGCCGGATAAGTCCCGGATCTCGGCGGTTCCGTTACAGGGAAGTAATCGAATCCTGCTCTCTTTCAAGAATGTGGATGACTTCAAATGGGCGATCAGATGTGGCCCTTGGTCTTACGAGAAAGTGCTGTTTGCGATTGCTGTCACTAATGCTCTTGAAGATCCCTTGAAGGTGTCTTTGTCGACGCAATATTTATGGATCCGCATACGGTGTCTTCCCCCGAAATACATGGATGAAGAGACTGGTGAAAGCATAAACTGCCTTGCAAAGGGGTCGGATGAGATATTGGAGCCGGCGTATGATGCTCTATTGAATGCGGAGAAGAAGGAGGCATGGTTTGCGAAGCAAGAGCAGGGTCTGAACTCACCAGAAACGGTGTCAGACGGTGGTGGTTGGCGTTTGGGGGCTGAGATGGAAGAAGAGGAGGCTCCAATGGTAGCTGGGGTTGATCAGATGGAGGTCGATAGAGCAGCTGGTTTGGAGCGGATACAGGGGGATGCGAAACTGCGGTGTGTAGGTGACATGGCGATGTACTGGCGATGGCCATTGCCGGATCCGACGTCGGACGCAGAGTTGACTCTGTGTGAGGTCCCAATTTGGGAATGCCTGGATTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1

Protein Analysis

215

Amino Acids

24.22

Weight (kDa)

4.51

Isoelectric Point (pI)

41.65

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 593
AccBSI CCGCTC 1 cut(s) 514
AccI GTMKAC 1 cut(s) 212
AciI CCGC 6 cut(s) 32, 56, 232, 347, 514, 538
AclWI GGATC 6 cut(s) 56, 185, 223, 236, 578, 591
AcoI YGGCCR 1 cut(s) 572
AcyI GRCGYC 1 cut(s) 590
AfaI GTAC 1 cut(s) 563
AfiI CCNNNNNNNGG 4 cut(s) 52, 68, 298, 628
AgsI TTSAA 5 cut(s) 97, 115, 188, 199, 343
AjnI CCWGG 1 cut(s) 636
AluBI AGCT 2 cut(s) 470, 503
AluI AGCT 2 cut(s) 470, 503
Alw26I GTCTC 1 cut(s) 263
AlwI GGATC 6 cut(s) 56, 185, 223, 236, 578, 591
AlwNI CAGNNNCTG 1 cut(s) 388
AoxI GGCC 3 cut(s) 28, 133, 572
ApeKI GCWGC 1 cut(s) 500
AspS9I GGNCC 3 cut(s) 29, 134, 619
AsuC2I CCSGG 1 cut(s) 46
AsuHPI GGTGA 3 cut(s) 287, 387, 560
AvaII GGWCC 1 cut(s) 619
BalI TGGCCA 1 cut(s) 574
BamHI GGATCC 2 cut(s) 228, 583
BbsI GAAGAC 1 cut(s) 236
BbvI GCAGC 1 cut(s) 512
BccI CCATC 4 cut(s) 20, 436, 478, 564
BcgI CGANNNNNNTGC 2 cut(s) 285, 319
BciT130I CCWGG 1 cut(s) 638
BciVI GTATCC 1 cut(s) 510
BclI TGATCA 1 cut(s) 478
BcnI CCSGG 1 cut(s) 46
BcoDI GTCTC 1 cut(s) 263
BfaI CTAG 1 cut(s) 646
BfmI CTRYAG 1 cut(s) 17
BfuI GTATCC 1 cut(s) 510
BisI GCNGC 1 cut(s) 501
BlsI GCNGC 1 cut(s) 502
Bme1390I CCNGG 2 cut(s) 46, 638
Bme18I GGWCC 1 cut(s) 619
BmgT120I GGNCC 3 cut(s) 29, 134, 619
BmiI GGNNCC 6 cut(s) 60, 230, 321, 459, 585, 621
BmrFI CCNGG 2 cut(s) 46, 638
BmsI GCATC 2 cut(s) 322, 517
BpiI GAAGAC 1 cut(s) 236
BpuMI CCSGG 1 cut(s) 46
BsaHI GRCGYC 1 cut(s) 590
BsaJI CCNNGG 1 cut(s) 137
BsaXI ACNNNNNCTCC 4 cut(s) 311, 341, 350, 380
Bsc4I CCNNNNNNNGG 4 cut(s) 52, 68, 298, 628
Bse118I RCCGGY 1 cut(s) 322
Bse1I ACTGG 2 cut(s) 277, 569
Bse3DI GCAATG 1 cut(s) 575
BseBI CCWGG 1 cut(s) 638
BseDI CCNNGG 1 cut(s) 137
BseGI GGATG 5 cut(s) 31, 112, 268, 313, 532
BseLI CCNNNNNNNGG 4 cut(s) 52, 68, 298, 628
BseMI GCAATG 1 cut(s) 575
BseMII CTCAG 1 cut(s) 429
BseNI ACTGG 2 cut(s) 277, 569
BseRI GAGGAG 1 cut(s) 467
BseXI GCAGC 1 cut(s) 512
BseYI CCCAGC 1 cut(s) 470
BshFI GGCC 3 cut(s) 30, 135, 574
BsiSI CCGG 4 cut(s) 35, 46, 323, 581
BslFI GGGAC 2 cut(s) 28, 605
BslI CCNNNNNNNGG 4 cut(s) 52, 68, 298, 628
BsmAI GTCTC 1 cut(s) 263
BsmFI GGGAC 2 cut(s) 28, 605
BsmI GAATGC 2 cut(s) 349, 638
BsnI GGCC 3 cut(s) 30, 135, 574
Bsp143I GATC 6 cut(s) 48, 123, 190, 228, 478, 583
BspACI CCGC 6 cut(s) 32, 56, 232, 347, 514, 538
BspANI GGCC 3 cut(s) 30, 135, 574
BspCNI CTCAG 1 cut(s) 430
BspLI GGNNCC 6 cut(s) 60, 230, 321, 459, 585, 621
BspPI GGATC 6 cut(s) 56, 185, 223, 236, 578, 591
BsrBI CCGCTC 1 cut(s) 514
BsrDI GCAATG 1 cut(s) 575
BsrFI RCCGGY 1 cut(s) 322
BsrI ACTGG 2 cut(s) 277, 569
BssAI RCCGGY 1 cut(s) 322
BssECI CCNNGG 1 cut(s) 137
BssMI GATC 6 cut(s) 48, 123, 190, 228, 478, 583
BssNI GRCGYC 1 cut(s) 590
BssT1I CCWWGG 1 cut(s) 137
Bst2UI CCWGG 1 cut(s) 638
Bst4CI ACNGT 3 cut(s) 240, 406, 416
Bst6I CTCTTC 2 cut(s) 261, 444
BstACI GRCGYC 1 cut(s) 590
BstAPI GCANNNNNTGC 2 cut(s) 288, 368
BstC8I GCNNGC 2 cut(s) 32, 324
BstDEI CTNAG 1 cut(s) 438
BstENI CCTNNNNNAGG 1 cut(s) 296
BstF5I GGATG 5 cut(s) 31, 112, 268, 313, 532
BstKTI GATC 6 cut(s) 51, 126, 193, 231, 481, 586
BstMAI GTCTC 1 cut(s) 263
BstMBI GATC 6 cut(s) 48, 123, 190, 228, 478, 583
BstMWI GCNNNNNNNGC 3 cut(s) 288, 332, 368
BstNI CCWGG 1 cut(s) 638
BstSCI CCNGG 2 cut(s) 44, 636
BstSFI CTRYAG 1 cut(s) 17
BstV1I GCAGC 1 cut(s) 512
BstV2I GAAGAC 1 cut(s) 236
BstX2I RGATCY 4 cut(s) 48, 190, 228, 583
BstYI RGATCY 4 cut(s) 48, 190, 228, 583
BsuI GTATCC 1 cut(s) 510
BsuRI GGCC 3 cut(s) 30, 135, 574
BtgZI GCGATG 2 cut(s) 572, 583
BtsCI GGATG 5 cut(s) 31, 112, 268, 313, 532
Cac8I GCNNGC 2 cut(s) 32, 324
CaiI CAGNNNCTG 1 cut(s) 388
Cfr10I RCCGGY 1 cut(s) 322
Cfr13I GGNCC 3 cut(s) 29, 134, 619
CseI GACGC 2 cut(s) 223, 605
Csp6I GTAC 1 cut(s) 562
CviAII CATG 3 cut(s) 259, 363, 553
CviQI GTAC 1 cut(s) 562
DdeI CTNAG 1 cut(s) 438
DpnI GATC 6 cut(s) 50, 125, 192, 230, 480, 585
DpnII GATC 6 cut(s) 48, 123, 190, 228, 478, 583
EaeI YGGCCR 1 cut(s) 572
Eam1104I CTCTTC 2 cut(s) 261, 444
EarI CTCTTC 2 cut(s) 261, 444
Eco130I CCWWGG 1 cut(s) 137
Eco47I GGWCC 1 cut(s) 619
EcoNI CCTNNNNNAGG 1 cut(s) 296
EcoO109I RGGNCCY 1 cut(s) 619
EcoRII CCWGG 1 cut(s) 636
EcoT14I CCWWGG 1 cut(s) 137
ErhI CCWWGG 1 cut(s) 137
FaeI CATG 3 cut(s) 262, 366, 556
FaiI YATR 7 cut(s) 226, 236, 260, 284, 330, 364, 554
FaqI GGGAC 2 cut(s) 28, 605
FatI CATG 3 cut(s) 258, 362, 552
FauI CCCGC 1 cut(s) 39
FbaI TGATCA 1 cut(s) 478
FblI GTMKAC 1 cut(s) 212
Fnu4HI GCNGC 1 cut(s) 501
FokI GGATG 5 cut(s) 38, 119, 275, 320, 539
Fsp4HI GCNGC 1 cut(s) 501
FspBI CTAG 1 cut(s) 646
GluI GCNGC 1 cut(s) 501
GsaI CCCAGC 1 cut(s) 474
HaeIII GGCC 3 cut(s) 30, 135, 574
HapII CCGG 4 cut(s) 35, 46, 323, 581
HgaI GACGC 2 cut(s) 223, 605
Hin1I GRCGYC 1 cut(s) 590
Hin1II CATG 3 cut(s) 262, 366, 556
HincII GTYRAC 2 cut(s) 213, 606
HindII GTYRAC 2 cut(s) 213, 606
HinfI GANTC 2 cut(s) 81, 607
HpaII CCGG 4 cut(s) 35, 46, 323, 581
HphI GGTGA 3 cut(s) 287, 387, 560
Hpy166II GTNNAC 2 cut(s) 213, 606
Hpy188I TCNGA 7 cut(s) 128, 307, 390, 412, 483, 588, 595
Hpy188III TCNNGA 2 cut(s) 97, 185
Hpy8I GTNNAC 2 cut(s) 213, 606
Hpy99I CGWCG 3 cut(s) 217, 592, 595
HpyAV CCTTC 3 cut(s) 16, 193, 349
HpyCH4III ACNGT 3 cut(s) 240, 406, 416
HpyCH4IV ACGT 1 cut(s) 590
HpyCH4V TGCA 1 cut(s) 296
HpyF10VI GCNNNNNNNGC 3 cut(s) 288, 332, 368
HpyF3I CTNAG 1 cut(s) 438
HpySE526I ACGT 1 cut(s) 590
Hsp92I GRCGYC 1 cut(s) 590
Hsp92II CATG 3 cut(s) 262, 366, 556
KroI GCCGGC 1 cut(s) 322
KroNI GCCGGC 1 cut(s) 324
Ksp22I TGATCA 1 cut(s) 478
Kzo9I GATC 6 cut(s) 48, 123, 190, 228, 478, 583
LmnI GCTCC 3 cut(s) 319, 463, 511
Lsp1109I GCAGC 1 cut(s) 512
LweI GCATC 2 cut(s) 322, 517
MaeI CTAG 1 cut(s) 646
MaeII ACGT 1 cut(s) 590
MaeIII GTNAC 3 cut(s) 63, 172, 548
MalI GATC 6 cut(s) 50, 125, 192, 230, 480, 585
MbiI CCGCTC 1 cut(s) 514
MboI GATC 6 cut(s) 48, 123, 190, 228, 478, 583
MboII GAAGA 6 cut(s) 200, 236, 278, 364, 458, 461
MflI RGATCY 4 cut(s) 48, 190, 228, 583
MlsI TGGCCA 1 cut(s) 574
MluCI AATT 1 cut(s) 624
MluNI TGGCCA 1 cut(s) 574
MlyI GAGTC 1 cut(s) 601
MmeI TCCRAC 3 cut(s) 285, 573, 611
MnlI CCTC 5 cut(s) 352, 445, 448, 481, 610
Mox20I TGGCCA 1 cut(s) 574
MroNI GCCGGC 1 cut(s) 322
MscI TGGCCA 1 cut(s) 574
MslI CAYNNNNRTG 1 cut(s) 557
Msp20I TGGCCA 1 cut(s) 574
MspA1I CMGCKG 1 cut(s) 503
MspI CCGG 4 cut(s) 35, 46, 323, 581
MspR9I CCNGG 2 cut(s) 46, 638
Mva1269I GAATGC 2 cut(s) 349, 638
MvaI CCWGG 1 cut(s) 638
MwoI GCNNNNNNNGC 3 cut(s) 288, 332, 368
NaeI GCCGGC 1 cut(s) 324
NciI CCSGG 1 cut(s) 46
NdeII GATC 6 cut(s) 48, 123, 190, 228, 478, 583
NgoMIV GCCGGC 1 cut(s) 322
NlaIII CATG 3 cut(s) 262, 366, 556
NlaIV GGNNCC 6 cut(s) 60, 230, 321, 459, 585, 621
NmeAIII GCCGAG 1 cut(s) 32
NmuCI GTSAC 2 cut(s) 172, 548
PcsI WCGNNNNNNNCGW 1 cut(s) 59
PctI GAATGC 2 cut(s) 349, 638
PdiI GCCGGC 1 cut(s) 324
PfeI GAWTC 1 cut(s) 81
PfoI TCCNGGA 1 cut(s) 44
PkrI GCNGC 1 cut(s) 502
PleI GAGTC 1 cut(s) 601
PpsI GAGTC 1 cut(s) 601
PpuMI RGGWCCY 1 cut(s) 619
Psp5II RGGWCCY 1 cut(s) 619
Psp6I CCWGG 1 cut(s) 636
PspFI CCCAGC 1 cut(s) 470
PspGI CCWGG 1 cut(s) 636
PspN4I GGNNCC 6 cut(s) 60, 230, 321, 459, 585, 621
PspPI GGNCC 3 cut(s) 29, 134, 619
PspPPI RGGWCCY 1 cut(s) 619
PstNI CAGNNNCTG 1 cut(s) 388
PsuI RGATCY 4 cut(s) 48, 190, 228, 583
PvuII CAGCTG 1 cut(s) 503
RsaI GTAC 1 cut(s) 563
RsaNI GTAC 1 cut(s) 562
RseI CAYNNNNRTG 1 cut(s) 557
SalI GTCGAC 1 cut(s) 211
SatI GCNGC 1 cut(s) 501
Sau3AI GATC 6 cut(s) 48, 123, 190, 228, 478, 583
Sau96I GGNCC 3 cut(s) 29, 134, 619
SchI GAGTC 1 cut(s) 601
ScrFI CCNGG 2 cut(s) 46, 638
SetI ASST 7 cut(s) 204, 472, 492, 505, 550, 593, 621
SfaNI GCATC 2 cut(s) 322, 517
SfcI CTRYAG 1 cut(s) 17
SinI GGWCC 1 cut(s) 619
SmiMI CAYNNNNRTG 1 cut(s) 557
Sse9I AATT 1 cut(s) 624
SsiI CCGC 6 cut(s) 32, 56, 232, 347, 514, 538
SspI AATATT 1 cut(s) 221
SspMI CTAG 1 cut(s) 646
StyD4I CCNGG 2 cut(s) 44, 636
StyI CCWWGG 1 cut(s) 137
TaaI ACNGT 3 cut(s) 240, 406, 416
TaiI ACGT 1 cut(s) 593
TaqI TCGA 3 cut(s) 79, 212, 492
TasI AATT 1 cut(s) 624
TatI WGTACW 1 cut(s) 561
TfiI GAWTC 1 cut(s) 81
TseFI GTSAC 2 cut(s) 172, 548
TseI GCWGC 1 cut(s) 500
Tsp45I GTSAC 2 cut(s) 172, 548
TspDTI ATGAA 1 cut(s) 279
TspGWI ACGGA 1 cut(s) 51
VpaK11BI GGWCC 1 cut(s) 619
XagI CCTNNNNNAGG 1 cut(s) 296
XmiI GTMKAC 1 cut(s) 212
XspI CTAG 1 cut(s) 646
ZraI GACGTC 1 cut(s) 591
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.