Rmu_sc0009295.1_g000004

zinc finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0009295.1
OG0017750
Physical Location & Seq
Forward (+)
20773 .. 25240
4468 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0009295.1_g000004.1.cds

Sequence Viewer

Length: 969 bp
atgacttccgagaactcaaaattagcccatgagtcccagaccaagactcccaaggcccccaaacccagtaatccaaaacccgccaaagtttttagcgcctatatcaaactagcaggatcaaacggcttccttggccaaaacgacgacgtcttcagctccgccgcgaccttctccagcagcatcccgctgatttccggcgccgagaattcattctcatggagaagaccagtctatgcggaagtgtgggagatgtggtgcaactggtcacataaaccttgcttctattgccaaagcgtgttcaacaatccccaggctgtaggggggcatctttgggtccatcaggaagagatcaaggaaatgaagagctggaactttcctggccatttcagcaactcagaaaccgttactagcaatactcaacctcccaccatctcccatcctgagaacttttctgaaggtgctgggagccatcaatctcgtttgctctcaaatggagtttgtcagttcaatactgatcaagttcaaaattttatggatagtatgccatccctttctgaaaatgccttgccatgctttcagaataactactgtggcaagcttccttctcttgtggatgctgtaacttttgatccagatctcagctcaaatcagtcgcctgccttcaaggagcgtaatattcttgatttctttaattgcaatctcagtcaatcttcaggccaaggtgaagctggtcaatctaagaaaaataatggaggtaagaggcgctgctcaggtgaagcccaaggaaatgctgggactgtgaatgagttgaaaaagaccaagatcaactcagatgagatggaaactgatagtcctcagaagagagagcttcttcttgttaaagaggaggacaactccaattctggttcaggaatgccttatgatgtggaagaaggcgagacaaaccttgatctgtcgctacatctgtaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

322

Amino Acids

35.48

Weight (kDa)

5.33

Isoelectric Point (pI)

44.76

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017750)

Species Orthologous Gene IDs
prunus_persica Prupe.4G081800_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0014311
rosa_laevigata RLG00000032097
rosa_multiflora Rmu_sc0009295.1_g000004
rosa_roxburghii Rroxscaffold_1G00062270
rosa_rugosa Rorug05G0017100 Rorug05G0017200 Rorug05G0017300
rosa_samantha Rh5AG111300 Rh5BG108600 Rh5CG120300 Rh5DG107300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 150
AccB1I GGYRCC 1 cut(s) 197
AccII CGCG 1 cut(s) 164
AciI CCGC 5 cut(s) 81, 159, 162, 185, 236
AclWI GGATC 2 cut(s) 124, 623
AcoI YGGCCR 2 cut(s) 133, 379
AcsI RAATTY 2 cut(s) 205, 526
AcuI CTGAAG 3 cut(s) 136, 474, 696
AcyI GRCGYC 2 cut(s) 147, 198
AgsI TTSAA 5 cut(s) 301, 508, 524, 664, 811
AjnI CCWGG 2 cut(s) 309, 376
AluBI AGCT 6 cut(s) 156, 366, 598, 642, 728, 868
AluI AGCT 6 cut(s) 156, 366, 598, 642, 728, 868
Alw26I GTCTC 1 cut(s) 932
AlwI GGATC 2 cut(s) 124, 623
AoxI GGCC 4 cut(s) 54, 133, 379, 715
ApeKI GCWGC 2 cut(s) 177, 765
ApoI RAATTY 2 cut(s) 205, 526
Asp700I GAANNNNTTC 1 cut(s) 209
AspLEI GCGC 3 cut(s) 98, 200, 765
AspS9I GGNCC 2 cut(s) 55, 334
AsuHPI GGTGA 2 cut(s) 734, 785
AvaII GGWCC 1 cut(s) 334
BalI TGGCCA 2 cut(s) 135, 381
BanI GGYRCC 1 cut(s) 197
BbsI GAAGAC 2 cut(s) 142, 229
BbvI GCAGC 2 cut(s) 189, 752
BccI CCATC 6 cut(s) 345, 437, 444, 477, 553, 832
BceAI ACGGC 1 cut(s) 139
BciT130I CCWGG 2 cut(s) 311, 378
BclI TGATCA 1 cut(s) 514
BcoDI GTCTC 1 cut(s) 932
BfaI CTAG 2 cut(s) 110, 408
BfmI CTRYAG 1 cut(s) 315
BfoI RGCGCY 3 cut(s) 99, 201, 766
BglII AGATCT 1 cut(s) 634
BisI GCNGC 3 cut(s) 162, 178, 766
BlsI GCNGC 3 cut(s) 163, 179, 767
Bme1390I CCNGG 2 cut(s) 311, 378
Bme18I GGWCC 1 cut(s) 334
BmgT120I GGNCC 2 cut(s) 55, 334
BmiI GGNNCC 4 cut(s) 57, 199, 335, 467
BmrFI CCNGG 2 cut(s) 311, 378
BmrI ACTGGG 1 cut(s) 60
BmsI GCATC 3 cut(s) 189, 334, 604
BmuI ACTGGG 1 cut(s) 60
BpiI GAAGAC 2 cut(s) 142, 229
BplI GAGNNNNNCTC 2 cut(s) 878, 910
BpmI CTGGAG 1 cut(s) 157
Bpu10I CCTNAGC 1 cut(s) 769
BsaBI GATNNNNATC 1 cut(s) 633
BsaHI GRCGYC 2 cut(s) 147, 198
BsaJI CCNNGG 5 cut(s) 51, 130, 309, 718, 781
BsaXI ACNNNNNCTCC 6 cut(s) 31, 61, 239, 269, 406, 436
Bse1I ACTGG 3 cut(s) 66, 227, 266
Bse8I GATNNNNATC 1 cut(s) 633
BseBI CCWGG 2 cut(s) 311, 378
BseDI CCNNGG 5 cut(s) 51, 130, 309, 718, 781
BseGI GGATG 4 cut(s) 180, 436, 545, 619
BseJI GATNNNNATC 1 cut(s) 633
BseMII CTCAG 7 cut(s) 408, 432, 652, 715, 783, 843, 869
BseNI ACTGG 3 cut(s) 66, 227, 266
BseRI GAGGAG 1 cut(s) 899
BseXI GCAGC 2 cut(s) 189, 752
BseYI CCCAGC 2 cut(s) 461, 791
Bsh1236I CGCG 1 cut(s) 164
BshFI GGCC 4 cut(s) 56, 135, 381, 717
BshNI GGYRCC 1 cut(s) 197
BsiSI CCGG 1 cut(s) 195
BslFI GGGAC 2 cut(s) 19, 808
BsmAI GTCTC 1 cut(s) 932
BsmFI GGGAC 2 cut(s) 19, 808
BsmI GAATGC 1 cut(s) 918
BsnI GGCC 4 cut(s) 56, 135, 381, 717
Bsp143I GATC 7 cut(s) 116, 348, 514, 628, 634, 822, 949
BspACI CCGC 5 cut(s) 81, 159, 162, 185, 236
BspANI GGCC 4 cut(s) 56, 135, 381, 717
BspCNI CTCAG 7 cut(s) 407, 433, 651, 714, 782, 842, 868
BspFNI CGCG 1 cut(s) 164
BspLI GGNNCC 4 cut(s) 57, 199, 335, 467
BspPI GGATC 2 cut(s) 124, 623
BspQI GCTCTTC 1 cut(s) 356
BspT107I GGYRCC 1 cut(s) 197
BsrI ACTGG 3 cut(s) 66, 227, 266
BssECI CCNNGG 5 cut(s) 51, 130, 309, 718, 781
BssMI GATC 7 cut(s) 116, 348, 514, 628, 634, 822, 949
BssNI GRCGYC 2 cut(s) 147, 198
BssT1I CCWWGG 4 cut(s) 51, 130, 718, 781
Bst2UI CCWGG 2 cut(s) 311, 378
Bst4CI ACNGT 3 cut(s) 403, 590, 799
Bst6I CTCTTC 3 cut(s) 339, 356, 854
BstACI GRCGYC 2 cut(s) 147, 198
BstC8I GCNNGC 2 cut(s) 596, 657
BstDEI CTNAG 8 cut(s) 394, 441, 638, 701, 738, 769, 829, 855
BstF5I GGATG 4 cut(s) 180, 436, 545, 619
BstFNI CGCG 1 cut(s) 164
BstH2I RGCGCY 3 cut(s) 99, 201, 766
BstHHI GCGC 3 cut(s) 98, 200, 765
BstKTI GATC 7 cut(s) 119, 351, 517, 631, 637, 825, 952
BstMAI GTCTC 1 cut(s) 932
BstMBI GATC 7 cut(s) 116, 348, 514, 628, 634, 822, 949
BstMWI GCNNNNNNNGC 3 cut(s) 132, 285, 387
BstNI CCWGG 2 cut(s) 311, 378
BstSCI CCNGG 2 cut(s) 309, 376
BstSFI CTRYAG 1 cut(s) 315
BstUI CGCG 1 cut(s) 164
BstV1I GCAGC 2 cut(s) 189, 752
BstV2I GAAGAC 2 cut(s) 142, 229
BstX2I RGATCY 1 cut(s) 634
BstYI RGATCY 1 cut(s) 634
BsuRI GGCC 4 cut(s) 56, 135, 381, 717
BtsCI GGATG 4 cut(s) 180, 436, 545, 619
Cac8I GCNNGC 2 cut(s) 596, 657
CfoI GCGC 3 cut(s) 98, 200, 765
Cfr13I GGNCC 2 cut(s) 55, 334
CviAII CATG 3 cut(s) 29, 216, 570
DdeI CTNAG 8 cut(s) 394, 441, 638, 701, 738, 769, 829, 855
DinI GGCGCC 1 cut(s) 199
DpnI GATC 7 cut(s) 118, 350, 516, 630, 636, 824, 951
DpnII GATC 7 cut(s) 116, 348, 514, 628, 634, 822, 949
EaeI YGGCCR 2 cut(s) 133, 379
Eam1104I CTCTTC 3 cut(s) 339, 356, 854
EarI CTCTTC 3 cut(s) 339, 356, 854
EciI GGCGGA 1 cut(s) 148
Eco130I CCWWGG 4 cut(s) 51, 130, 718, 781
Eco47I GGWCC 1 cut(s) 334
Eco57I CTGAAG 3 cut(s) 136, 474, 696
EcoO109I RGGNCCY 1 cut(s) 55
EcoRI GAATTC 1 cut(s) 205
EcoRII CCWGG 2 cut(s) 309, 376
EcoT14I CCWWGG 4 cut(s) 51, 130, 718, 781
EgeI GGCGCC 1 cut(s) 199
EheI GGCGCC 1 cut(s) 199
ErhI CCWWGG 4 cut(s) 51, 130, 718, 781
FaeI CATG 3 cut(s) 32, 219, 573
FaiI YATR 9 cut(s) 30, 102, 217, 234, 270, 533, 542, 571, 921
FaqI GGGAC 2 cut(s) 19, 808
FatI CATG 3 cut(s) 28, 215, 569
FauI CCCGC 2 cut(s) 88, 192
FbaI TGATCA 1 cut(s) 514
Fnu4HI GCNGC 3 cut(s) 162, 178, 766
FokI GGATG 4 cut(s) 167, 423, 532, 626
Fsp4HI GCNGC 3 cut(s) 162, 178, 766
FspBI CTAG 2 cut(s) 110, 408
GlaI GCGC 3 cut(s) 97, 199, 764
GluI GCNGC 3 cut(s) 162, 178, 766
GsaI CCCAGC 2 cut(s) 465, 795
GsuI CTGGAG 1 cut(s) 157
HaeII RGCGCY 3 cut(s) 99, 201, 766
HaeIII GGCC 4 cut(s) 56, 135, 381, 717
HapII CCGG 1 cut(s) 195
HhaI GCGC 3 cut(s) 98, 200, 765
Hin1I GRCGYC 2 cut(s) 147, 198
Hin1II CATG 3 cut(s) 32, 219, 573
Hin6I GCGC 3 cut(s) 96, 198, 763
HinP1I GCGC 3 cut(s) 96, 198, 763
HindIII AAGCTT 1 cut(s) 596
HinfI GANTC 2 cut(s) 32, 46
HpaII CCGG 1 cut(s) 195
HphI GGTGA 2 cut(s) 734, 785
Hpy188I TCNGA 7 cut(s) 10, 397, 454, 556, 579, 832, 858
Hpy188III TCNNGA 5 cut(s) 341, 440, 632, 680, 909
Hpy99I CGWCG 2 cut(s) 146, 149
HpyAV CCTTC 5 cut(s) 178, 449, 612, 670, 926
HpyCH4III ACNGT 3 cut(s) 403, 590, 799
HpyCH4IV ACGT 1 cut(s) 147
HpyCH4V TGCA 2 cut(s) 258, 696
HpyF10VI GCNNNNNNNGC 3 cut(s) 132, 285, 387
HpyF3I CTNAG 8 cut(s) 394, 441, 638, 701, 738, 769, 829, 855
HpySE526I ACGT 1 cut(s) 147
Hsp92I GRCGYC 2 cut(s) 147, 198
Hsp92II CATG 3 cut(s) 32, 219, 573
HspAI GCGC 3 cut(s) 96, 198, 763
KasI GGCGCC 1 cut(s) 197
Ksp22I TGATCA 1 cut(s) 514
Kzo9I GATC 7 cut(s) 116, 348, 514, 628, 634, 822, 949
LguI GCTCTTC 1 cut(s) 356
LmnI GCTCC 3 cut(s) 161, 465, 667
Lsp1109I GCAGC 2 cut(s) 189, 752
LweI GCATC 3 cut(s) 189, 334, 604
MaeI CTAG 2 cut(s) 110, 408
MaeII ACGT 1 cut(s) 147
MaeIII GTNAC 3 cut(s) 264, 403, 619
MalI GATC 7 cut(s) 118, 350, 516, 630, 636, 824, 951
MboI GATC 7 cut(s) 116, 348, 514, 628, 634, 822, 949
MboII GAAGA 8 cut(s) 142, 234, 356, 373, 702, 863, 871, 941
MflI RGATCY 1 cut(s) 634
MlsI TGGCCA 2 cut(s) 135, 381
MluCI AATT 5 cut(s) 20, 205, 526, 691, 898
MluNI TGGCCA 2 cut(s) 135, 381
Mly113I GGCGCC 1 cut(s) 198
MlyI GAGTC 2 cut(s) 40, 41
MnlI CCTC 6 cut(s) 432, 746, 753, 864, 877, 880
Mox20I TGGCCA 2 cut(s) 135, 381
MroXI GAANNNNTTC 1 cut(s) 209
MscI TGGCCA 2 cut(s) 135, 381
MseI TTAA 2 cut(s) 690, 879
MslI CAYNNNNRTG 1 cut(s) 214
Msp20I TGGCCA 2 cut(s) 135, 381
MspA1I CMGCKG 1 cut(s) 187
MspI CCGG 1 cut(s) 195
MspR9I CCNGG 2 cut(s) 311, 378
Mva1269I GAATGC 1 cut(s) 918
MvaI CCWGG 2 cut(s) 311, 378
MvnI CGCG 1 cut(s) 164
MwoI GCNNNNNNNGC 3 cut(s) 132, 285, 387
NarI GGCGCC 1 cut(s) 198
NdeII GATC 7 cut(s) 116, 348, 514, 628, 634, 822, 949
NlaIII CATG 3 cut(s) 32, 219, 573
NlaIV GGNNCC 4 cut(s) 57, 199, 335, 467
NmeAIII GCCGAG 1 cut(s) 226
NmuCI GTSAC 1 cut(s) 264
PciSI GCTCTTC 1 cut(s) 356
PctI GAATGC 1 cut(s) 918
PdmI GAANNNNTTC 1 cut(s) 209
PflFI GACNNNGTC 1 cut(s) 146
PkrI GCNGC 3 cut(s) 163, 179, 767
PleI GAGTC 2 cut(s) 40, 40
PluTI GGCGCC 1 cut(s) 201
PpsI GAGTC 2 cut(s) 40, 40
Psp6I CCWGG 2 cut(s) 309, 376
PspFI CCCAGC 2 cut(s) 461, 791
PspGI CCWGG 2 cut(s) 309, 376
PspN4I GGNNCC 4 cut(s) 57, 199, 335, 467
PspPI GGNCC 2 cut(s) 55, 334
PsuI RGATCY 1 cut(s) 634
PsyI GACNNNGTC 1 cut(s) 146
RseI CAYNNNNRTG 1 cut(s) 214
SapI GCTCTTC 1 cut(s) 356
SaqAI TTAA 2 cut(s) 690, 879
SatI GCNGC 3 cut(s) 162, 178, 766
Sau3AI GATC 7 cut(s) 116, 348, 514, 628, 634, 822, 949
Sau96I GGNCC 2 cut(s) 55, 334
SchI GAGTC 2 cut(s) 40, 41
ScrFI CCNGG 2 cut(s) 311, 378
SfaNI GCATC 3 cut(s) 189, 334, 604
SfcI CTRYAG 1 cut(s) 315
SfoI GGCGCC 1 cut(s) 199
SinI GGWCC 1 cut(s) 334
SmiMI CAYNNNNRTG 1 cut(s) 214
Sse9I AATT 5 cut(s) 20, 205, 526, 691, 898
SsiI CCGC 5 cut(s) 81, 159, 162, 185, 236
SspDI GGCGCC 1 cut(s) 197
SspI AATATT 1 cut(s) 676
SspMI CTAG 2 cut(s) 110, 408
StyD4I CCNGG 2 cut(s) 309, 376
StyI CCWWGG 4 cut(s) 51, 130, 718, 781
TaaI ACNGT 3 cut(s) 403, 590, 799
TaiI ACGT 1 cut(s) 150
TasI AATT 5 cut(s) 20, 205, 526, 691, 898
TauI GCSGC 1 cut(s) 164
Tru1I TTAA 2 cut(s) 690, 879
Tru9I TTAA 2 cut(s) 690, 879
TseFI GTSAC 1 cut(s) 264
TseI GCWGC 2 cut(s) 177, 765
Tsp45I GTSAC 1 cut(s) 264
TspDTI ATGAA 2 cut(s) 198, 374
Tth111I GACNNNGTC 1 cut(s) 146
VpaK11BI GGWCC 1 cut(s) 334
XapI RAATTY 2 cut(s) 205, 526
XcmI CCANNNNNNNNNTGG 2 cut(s) 725, 788
XmnI GAANNNNTTC 1 cut(s) 209
XspI CTAG 2 cut(s) 110, 408
ZraI GACGTC 1 cut(s) 148
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.