Rh5BG108600

zinc finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0017750
Physical Location & Seq
Reverse (-)
10141706 .. 10151226
9521 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG108600.1

Sequence Viewer

Length: 1122 bp
ATGAAAACTCAAGAATATTATGGAATTTTAGACTTGAGTCCAACACCCAATGTGCTAGAGAAAGGAGAGGCCCATAAGTCTGAGACTCCCAAGGCGCAACAGGCCCATGAGTCCCAGACCAAGACTCCCAAGGCCCCCAAACCCACTAATCCAAAACCCGCCAAAGTTTTTAGCGCCTATATCAAACTAGCAGGATCAAACGGCTTCCTTGGCCAAAACGACGACGTCTTCAGCTCCGCCACGACCTTCTCCAGCAGCATCCCGCTGATTTCCGGCGCCGCTACCCCGGTAACGGGTATAAATAGAGGTAAGGAGCAAGGCACTCTTCCTCGTCCCTCCATTGTTATTGCTCCTGCTAGCTTTACTTCTTCTTGCAAGAATTGTATTAAGGTTTTCCTTTTGACCCCTAACCTTGAAAAGGGTGTACAGTGCTTCTATTGCCAAAGCGTGTTCAACAATCCCCAGGCTGTAGGGGGGCATCTTCGGGTCCATCAGGAAGAGATCAAGGAAATGAAGAGCTGGAACTTTCCTGGCCATTTCAGTAACTCAGAAATCGTTACTAGCAATACTCAACCTCTCACCATCTCCCATCCTGAGAACTTTTCTGAAGGTGCTGGGAGCCATCAATCTCGTTTGCTCTCAAATGGAGTTTGTCAGTTCAATACTGATCAAGTTCAAAATTTTATGGATAGTATGCCATCCCTTTCTGAAAATGCCTTGCCATGCTTTCAGAATAACTACTGTGGCAAGCTTCCTTCTCTTGTGGATGCTGTAACTTTTGATCCAGATTTCAGCTCAAATCAGTCGCCTGCCTTCAAGGAGGGTAATATTCTTGATTTCTTTAATTGCAATCTCAGTCAATCTTCAGGCCAAGGTGAAGCTGGTCAATCTAAGAAAAATAATGGAGGTAAGAGGCGCTGCTCAGGTGAAGCCCAAGGAAATGCTGGGACTGTGAATGAGTTGAAAAGGACCAAGATTAACTCAGATGAGATGGAAACTGATAGTCCTCAGAAGAAAGAGCTTCTTCTTGTTAAAGAGGAGGACAACTCCAATTCTGGTTCAGGAATGCCTTATGATGTGGAAGAAGGCGAGACAAACCTTGATCTGTCGCTACATCTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

373

Amino Acids

40.44

Weight (kDa)

5.69

Isoelectric Point (pI)

40.18

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017750)

Species Orthologous Gene IDs
prunus_persica Prupe.4G081800_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0014311
rosa_laevigata RLG00000032097
rosa_multiflora Rmu_sc0009295.1_g000004
rosa_roxburghii Rroxscaffold_1G00062270
rosa_rugosa Rorug05G0017100 Rorug05G0017200 Rorug05G0017300
rosa_samantha Rh5AG111300 Rh5BG108600 Rh5CG120300 Rh5DG107300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 228
AccB1I GGYRCC 1 cut(s) 275
AciI CCGC 4 cut(s) 159, 237, 263, 279
AclWI GGATC 2 cut(s) 202, 776
AcoI YGGCCR 2 cut(s) 211, 532
AcsI RAATTY 2 cut(s) 24, 679
AcuI CTGAAG 3 cut(s) 214, 627, 849
AcyI GRCGYC 2 cut(s) 225, 276
AfaI GTAC 1 cut(s) 426
AfiI CCNNNNNNNGG 3 cut(s) 292, 293, 418
AgsI TTSAA 6 cut(s) 416, 454, 661, 677, 817, 964
AjnI CCWGG 2 cut(s) 462, 529
AluBI AGCT 7 cut(s) 234, 360, 519, 751, 795, 881, 1021
AluI AGCT 7 cut(s) 234, 360, 519, 751, 795, 881, 1021
Alw26I GTCTC 2 cut(s) 77, 1085
AlwI GGATC 2 cut(s) 202, 776
AoxI GGCC 6 cut(s) 69, 102, 132, 211, 532, 868
ApeKI GCWGC 2 cut(s) 255, 918
ApoI RAATTY 2 cut(s) 24, 679
AspLEI GCGC 4 cut(s) 97, 176, 278, 918
AspS9I GGNCC 5 cut(s) 70, 103, 133, 487, 969
AsuC2I CCSGG 1 cut(s) 287
AsuHPI GGTGA 3 cut(s) 571, 887, 938
AsuNHI GCTAGC 1 cut(s) 356
AvaII GGWCC 2 cut(s) 487, 969
BalI TGGCCA 2 cut(s) 213, 534
BanI GGYRCC 1 cut(s) 275
BbsI GAAGAC 1 cut(s) 220
BbvI GCAGC 2 cut(s) 267, 905
BccI CCATC 6 cut(s) 498, 590, 597, 630, 706, 985
BceAI ACGGC 1 cut(s) 217
BciT130I CCWGG 2 cut(s) 464, 531
BclI TGATCA 1 cut(s) 667
BcnI CCSGG 1 cut(s) 287
BcoDI GTCTC 2 cut(s) 77, 1085
BfaI CTAG 4 cut(s) 56, 188, 357, 561
BfmI CTRYAG 2 cut(s) 468, 1118
BfoI RGCGCY 3 cut(s) 177, 279, 919
BisI GCNGC 3 cut(s) 256, 279, 919
BlsI GCNGC 3 cut(s) 257, 280, 920
Bme1390I CCNGG 3 cut(s) 287, 464, 531
Bme18I GGWCC 2 cut(s) 487, 969
BmgT120I GGNCC 5 cut(s) 70, 103, 133, 487, 969
BmiI GGNNCC 4 cut(s) 135, 277, 488, 620
BmrFI CCNGG 3 cut(s) 287, 464, 531
BmsI GCATC 3 cut(s) 267, 487, 757
BmtI GCTAGC 1 cut(s) 360
BoxI GACNNNNGTC 1 cut(s) 36
BpiI GAAGAC 1 cut(s) 220
BplI GAGNNNNNCTC 2 cut(s) 1031, 1063
BpmI CTGGAG 1 cut(s) 235
Bpu10I CCTNAGC 1 cut(s) 922
BpuEI CTTGAG 1 cut(s) 55
BpuMI CCSGG 1 cut(s) 287
BsaHI GRCGYC 2 cut(s) 225, 276
BsaJI CCNNGG 7 cut(s) 90, 129, 208, 285, 462, 871, 934
BsaXI ACNNNNNCTCC 2 cut(s) 109, 139
Bsc4I CCNNNNNNNGG 3 cut(s) 292, 293, 418
BseBI CCWGG 2 cut(s) 464, 531
BseDI CCNNGG 7 cut(s) 90, 129, 208, 285, 462, 871, 934
BseGI GGATG 4 cut(s) 258, 589, 698, 772
BseLI CCNNNNNNNGG 3 cut(s) 292, 293, 418
BseMII CTCAG 7 cut(s) 72, 561, 585, 868, 936, 996, 1022
BseRI GAGGAG 1 cut(s) 1052
BseXI GCAGC 2 cut(s) 267, 905
BseYI CCCAGC 2 cut(s) 614, 944
BshFI GGCC 6 cut(s) 71, 104, 134, 213, 534, 870
BshNI GGYRCC 1 cut(s) 275
BsiSI CCGG 2 cut(s) 273, 287
BslFI GGGAC 3 cut(s) 97, 318, 961
BslI CCNNNNNNNGG 3 cut(s) 292, 293, 418
BsmAI GTCTC 2 cut(s) 77, 1085
BsmFI GGGAC 3 cut(s) 97, 318, 961
BsmI GAATGC 1 cut(s) 1071
BsnI GGCC 6 cut(s) 71, 104, 134, 213, 534, 870
Bsp1407I TGTACA 1 cut(s) 424
Bsp143I GATC 5 cut(s) 194, 501, 667, 781, 1102
BspACI CCGC 4 cut(s) 159, 237, 263, 279
BspANI GGCC 6 cut(s) 71, 104, 134, 213, 534, 870
BspCNI CTCAG 7 cut(s) 73, 560, 586, 867, 935, 995, 1021
BspLI GGNNCC 4 cut(s) 135, 277, 488, 620
BspOI GCTAGC 1 cut(s) 360
BspPI GGATC 2 cut(s) 202, 776
BspQI GCTCTTC 1 cut(s) 509
BspT107I GGYRCC 1 cut(s) 275
BsrGI TGTACA 1 cut(s) 424
BssECI CCNNGG 7 cut(s) 90, 129, 208, 285, 462, 871, 934
BssMI GATC 5 cut(s) 194, 501, 667, 781, 1102
BssNI GRCGYC 2 cut(s) 225, 276
BssT1I CCWWGG 5 cut(s) 90, 129, 208, 871, 934
Bst2UI CCWGG 2 cut(s) 464, 531
Bst4CI ACNGT 3 cut(s) 429, 743, 952
Bst6I CTCTTC 3 cut(s) 330, 492, 509
BstACI GRCGYC 2 cut(s) 225, 276
BstAUI TGTACA 1 cut(s) 424
BstC8I GCNNGC 3 cut(s) 358, 749, 810
BstDEI CTNAG 8 cut(s) 81, 547, 594, 854, 891, 922, 982, 1008
BstENI CCTNNNNNAGG 1 cut(s) 416
BstF5I GGATG 4 cut(s) 258, 589, 698, 772
BstH2I RGCGCY 3 cut(s) 177, 279, 919
BstHHI GCGC 4 cut(s) 97, 176, 278, 918
BstKTI GATC 5 cut(s) 197, 504, 670, 784, 1105
BstMAI GTCTC 2 cut(s) 77, 1085
BstMBI GATC 5 cut(s) 194, 501, 667, 781, 1102
BstMWI GCNNNNNNNGC 3 cut(s) 101, 210, 438
BstNI CCWGG 2 cut(s) 464, 531
BstPAI GACNNNNGTC 1 cut(s) 36
BstSCI CCNGG 3 cut(s) 285, 462, 529
BstSFI CTRYAG 2 cut(s) 468, 1118
BstV1I GCAGC 2 cut(s) 267, 905
BstV2I GAAGAC 1 cut(s) 220
BsuRI GGCC 6 cut(s) 71, 104, 134, 213, 534, 870
BtsCI GGATG 4 cut(s) 258, 589, 698, 772
BtsIMutI CAGTG 1 cut(s) 434
Cac8I GCNNGC 3 cut(s) 358, 749, 810
CfoI GCGC 4 cut(s) 97, 176, 278, 918
Cfr13I GGNCC 5 cut(s) 70, 103, 133, 487, 969
Csp6I GTAC 1 cut(s) 425
CviAII CATG 2 cut(s) 107, 723
CviQI GTAC 1 cut(s) 425
DdeI CTNAG 8 cut(s) 81, 547, 594, 854, 891, 922, 982, 1008
DinI GGCGCC 1 cut(s) 277
DpnI GATC 5 cut(s) 196, 503, 669, 783, 1104
DpnII GATC 5 cut(s) 194, 501, 667, 781, 1102
EaeI YGGCCR 2 cut(s) 211, 532
Eam1104I CTCTTC 3 cut(s) 330, 492, 509
EarI CTCTTC 3 cut(s) 330, 492, 509
EciI GGCGGA 1 cut(s) 226
Eco130I CCWWGG 5 cut(s) 90, 129, 208, 871, 934
Eco47I GGWCC 2 cut(s) 487, 969
Eco57I CTGAAG 3 cut(s) 214, 627, 849
EcoNI CCTNNNNNAGG 1 cut(s) 416
EcoO109I RGGNCCY 1 cut(s) 133
EcoRII CCWGG 2 cut(s) 462, 529
EcoT14I CCWWGG 5 cut(s) 90, 129, 208, 871, 934
EgeI GGCGCC 1 cut(s) 277
EheI GGCGCC 1 cut(s) 277
ErhI CCWWGG 5 cut(s) 90, 129, 208, 871, 934
FaeI CATG 2 cut(s) 110, 726
FaiI YATR 9 cut(s) 21, 75, 108, 180, 299, 686, 695, 724, 1074
FalI AAGNNNNNCTT 4 cut(s) 309, 341, 1008, 1040
FaqI GGGAC 3 cut(s) 97, 318, 961
FatI CATG 2 cut(s) 106, 722
FauI CCCGC 2 cut(s) 166, 270
FbaI TGATCA 1 cut(s) 667
Fnu4HI GCNGC 3 cut(s) 256, 279, 919
FokI GGATG 4 cut(s) 245, 576, 685, 779
Fsp4HI GCNGC 3 cut(s) 256, 279, 919
FspBI CTAG 4 cut(s) 56, 188, 357, 561
GlaI GCGC 4 cut(s) 96, 175, 277, 917
GluI GCNGC 3 cut(s) 256, 279, 919
GsaI CCCAGC 2 cut(s) 618, 948
GsuI CTGGAG 1 cut(s) 235
HaeII RGCGCY 3 cut(s) 177, 279, 919
HaeIII GGCC 6 cut(s) 71, 104, 134, 213, 534, 870
HapII CCGG 2 cut(s) 273, 287
HhaI GCGC 4 cut(s) 97, 176, 278, 918
Hin1I GRCGYC 2 cut(s) 225, 276
Hin1II CATG 2 cut(s) 110, 726
Hin6I GCGC 4 cut(s) 95, 174, 276, 916
HinP1I GCGC 4 cut(s) 95, 174, 276, 916
HindIII AAGCTT 1 cut(s) 749
HinfI GANTC 4 cut(s) 37, 85, 110, 124
HpaII CCGG 2 cut(s) 273, 287
HphI GGTGA 3 cut(s) 571, 887, 938
Hpy166II GTNNAC 1 cut(s) 425
Hpy188I TCNGA 7 cut(s) 82, 550, 607, 709, 732, 985, 1011
Hpy188III TCNNGA 6 cut(s) 11, 494, 593, 785, 833, 1062
Hpy8I GTNNAC 1 cut(s) 425
Hpy99I CGWCG 2 cut(s) 224, 227
HpyAV CCTTC 5 cut(s) 256, 602, 765, 823, 1079
HpyCH4III ACNGT 3 cut(s) 429, 743, 952
HpyCH4IV ACGT 1 cut(s) 225
HpyCH4V TGCA 2 cut(s) 375, 849
HpyF10VI GCNNNNNNNGC 3 cut(s) 101, 210, 438
HpyF3I CTNAG 8 cut(s) 81, 547, 594, 854, 891, 922, 982, 1008
HpySE526I ACGT 1 cut(s) 225
Hsp92I GRCGYC 2 cut(s) 225, 276
Hsp92II CATG 2 cut(s) 110, 726
HspAI GCGC 4 cut(s) 95, 174, 276, 916
KasI GGCGCC 1 cut(s) 275
Ksp22I TGATCA 1 cut(s) 667
Kzo9I GATC 5 cut(s) 194, 501, 667, 781, 1102
LguI GCTCTTC 1 cut(s) 509
LmnI GCTCC 4 cut(s) 239, 313, 355, 618
Lsp1109I GCAGC 2 cut(s) 267, 905
LweI GCATC 3 cut(s) 267, 487, 757
MaeI CTAG 4 cut(s) 56, 188, 357, 561
MaeII ACGT 1 cut(s) 225
MaeIII GTNAC 4 cut(s) 289, 542, 556, 772
MalI GATC 5 cut(s) 196, 503, 669, 783, 1104
MboI GATC 5 cut(s) 194, 501, 667, 781, 1102
MlsI TGGCCA 2 cut(s) 213, 534
MluCI AATT 5 cut(s) 24, 379, 679, 844, 1051
MluNI TGGCCA 2 cut(s) 213, 534
Mly113I GGCGCC 1 cut(s) 276
MlyI GAGTC 4 cut(s) 46, 79, 118, 119
MmeI TCCRAC 1 cut(s) 65
Mox20I TGGCCA 2 cut(s) 213, 534
MscI TGGCCA 2 cut(s) 213, 534
MseI TTAA 4 cut(s) 387, 843, 978, 1032
Msp20I TGGCCA 2 cut(s) 213, 534
MspA1I CMGCKG 1 cut(s) 265
MspI CCGG 2 cut(s) 273, 287
MspR9I CCNGG 3 cut(s) 287, 464, 531
Mva1269I GAATGC 1 cut(s) 1071
MvaI CCWGG 2 cut(s) 464, 531
MwoI GCNNNNNNNGC 3 cut(s) 101, 210, 438
NarI GGCGCC 1 cut(s) 276
NciI CCSGG 1 cut(s) 287
NdeII GATC 5 cut(s) 194, 501, 667, 781, 1102
NheI GCTAGC 1 cut(s) 356
NlaIII CATG 2 cut(s) 110, 726
NlaIV GGNNCC 4 cut(s) 135, 277, 488, 620
PciSI GCTCTTC 1 cut(s) 509
PctI GAATGC 1 cut(s) 1071
PflFI GACNNNGTC 1 cut(s) 224
PkrI GCNGC 3 cut(s) 257, 280, 920
PleI GAGTC 4 cut(s) 45, 79, 118, 118
PluTI GGCGCC 1 cut(s) 279
PpsI GAGTC 4 cut(s) 45, 79, 118, 118
PshAI GACNNNNGTC 1 cut(s) 36
Psp6I CCWGG 2 cut(s) 462, 529
PspFI CCCAGC 2 cut(s) 614, 944
PspGI CCWGG 2 cut(s) 462, 529
PspN4I GGNNCC 4 cut(s) 135, 277, 488, 620
PspPI GGNCC 5 cut(s) 70, 103, 133, 487, 969
PsyI GACNNNGTC 1 cut(s) 224
RsaI GTAC 1 cut(s) 426
RsaNI GTAC 1 cut(s) 425
SapI GCTCTTC 1 cut(s) 509
SaqAI TTAA 4 cut(s) 387, 843, 978, 1032
SatI GCNGC 3 cut(s) 256, 279, 919
Sau3AI GATC 5 cut(s) 194, 501, 667, 781, 1102
Sau96I GGNCC 5 cut(s) 70, 103, 133, 487, 969
SchI GAGTC 4 cut(s) 46, 79, 118, 119
ScrFI CCNGG 3 cut(s) 287, 464, 531
SfaNI GCATC 3 cut(s) 267, 487, 757
SfcI CTRYAG 2 cut(s) 468, 1118
SfoI GGCGCC 1 cut(s) 277
SinI GGWCC 2 cut(s) 487, 969
SmlI CTYRAG 2 cut(s) 9, 34
SmoI CTYRAG 2 cut(s) 9, 34
Sse9I AATT 5 cut(s) 24, 379, 679, 844, 1051
SsiI CCGC 4 cut(s) 159, 237, 263, 279
SspDI GGCGCC 1 cut(s) 275
SspI AATATT 2 cut(s) 17, 829
SspMI CTAG 4 cut(s) 56, 188, 357, 561
StyD4I CCNGG 3 cut(s) 285, 462, 529
StyI CCWWGG 5 cut(s) 90, 129, 208, 871, 934
TaaI ACNGT 3 cut(s) 429, 743, 952
TaiI ACGT 1 cut(s) 228
TasI AATT 5 cut(s) 24, 379, 679, 844, 1051
TatI WGTACW 1 cut(s) 424
TauI GCSGC 1 cut(s) 281
Tru1I TTAA 4 cut(s) 387, 843, 978, 1032
Tru9I TTAA 4 cut(s) 387, 843, 978, 1032
TscAI CASTG 1 cut(s) 434
TseI GCWGC 2 cut(s) 255, 918
TspDTI ATGAA 2 cut(s) 17, 527
TspRI CASTG 1 cut(s) 434
Tth111I GACNNNGTC 1 cut(s) 224
VpaK11BI GGWCC 2 cut(s) 487, 969
XagI CCTNNNNNAGG 1 cut(s) 416
XapI RAATTY 2 cut(s) 24, 679
XcmI CCANNNNNNNNNTGG 2 cut(s) 878, 941
XspI CTAG 4 cut(s) 56, 188, 357, 561
ZraI GACGTC 1 cut(s) 226
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.