Rroxscaffold_1G00062270

zinc finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0017750
Physical Location & Seq
Forward (+)
84325166 .. 84325996
831 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00062270.1

Sequence Viewer

Length: 831 bp
ATGGGTATAAATAGAGGTAAGGAGCAAGGCACTCTTCCTCGTCCCTCCATTGTTGTTGCTCCTGCTAGCTTTACTTCTTCTTGCAAGAATTCTATTAAGGTTTTCGTTTTGACCCCTAACCTTGAAAAGGGTGTACAGTGCTTCTATTGCCAAAGCGTGTTCAACAATCCCCAGGCTGTAGGGGGGCATCTTCGGGTCCATCAGGAAGAGATCAAGGAAATGAAGAGCTGGAACTTTCCTGGCCATTTCAGTAACTCAGAAAGCGTTACTAGCAATACTCAACCTCTCACCATCTCCCATCCTGAGAACTTTTCTGAAGGTGCTGGGAGCCATCAATCTCGTTTGCTCTCAAATGGAGTTTGTCAGTTCAATACTGATCAAGTTCAAAATTTTATGGATAGTATGCCATCCCCTTCTGAAAATGCCTTGCCATGCTTTCAGAATAACTACTGTGGCAAGCTTCCTTCTCTTGTGGATGCTGTAACTTTTGATCCAGATCTCAGCTCAAATCAGTCGCCTGCCTTCAAGGAGCGTAACATTCTTGATTTCTTTAATTGCAATCTCAGTCAATCTTCAGGCCAAGGTGAAGCTGGTCAATCTAAGAAAAATAATGGAGGTAAGAGGCACTGCTCAGGTGAAGCCCAAGGAAATGCTGGGACTGTGAATGAGTTGAAAAGGACCAAGATCAACTCAGATGAGATGGAAACTGATAGTCCTCATAAGAGAGAGCTTCTTCTTGTTAAAGAGGAGGACAACTCCAATTCTGGTTCAGGAATGCCTTATGATGTGGAAGAAGGCGAGACAAACCTTGATCTGTCGCTACATCTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

276

Amino Acids

30.19

Weight (kDa)

5.39

Isoelectric Point (pI)

48.54

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017750)

Species Orthologous Gene IDs
prunus_persica Prupe.4G081800_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0014311
rosa_laevigata RLG00000032097
rosa_multiflora Rmu_sc0009295.1_g000004
rosa_roxburghii Rroxscaffold_1G00062270
rosa_rugosa Rorug05G0017100 Rorug05G0017200 Rorug05G0017300
rosa_samantha Rh5AG111300 Rh5BG108600 Rh5CG120300 Rh5DG107300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 485
AcoI YGGCCR 1 cut(s) 241
AcsI RAATTY 2 cut(s) 88, 388
AcuI CTGAAG 2 cut(s) 336, 558
AfaI GTAC 1 cut(s) 135
AfiI CCNNNNNNNGG 1 cut(s) 127
AgsI TTSAA 6 cut(s) 125, 163, 370, 386, 526, 673
AjnI CCWGG 2 cut(s) 171, 238
AluBI AGCT 6 cut(s) 69, 228, 460, 504, 590, 730
AluI AGCT 6 cut(s) 69, 228, 460, 504, 590, 730
Alw26I GTCTC 1 cut(s) 794
AlwI GGATC 1 cut(s) 485
AoxI GGCC 2 cut(s) 241, 577
ApoI RAATTY 2 cut(s) 88, 388
AspS9I GGNCC 2 cut(s) 196, 678
AsuHPI GGTGA 3 cut(s) 280, 596, 647
AsuNHI GCTAGC 1 cut(s) 65
AvaII GGWCC 2 cut(s) 196, 678
BalI TGGCCA 1 cut(s) 243
BccI CCATC 6 cut(s) 207, 299, 306, 339, 415, 694
BciT130I CCWGG 2 cut(s) 173, 240
BclI TGATCA 1 cut(s) 376
BcoDI GTCTC 1 cut(s) 794
BfaI CTAG 2 cut(s) 66, 270
BfmI CTRYAG 2 cut(s) 177, 827
BglII AGATCT 1 cut(s) 496
Bme1390I CCNGG 2 cut(s) 173, 240
Bme18I GGWCC 2 cut(s) 196, 678
BmgT120I GGNCC 2 cut(s) 196, 678
BmiI GGNNCC 2 cut(s) 197, 329
BmrFI CCNGG 2 cut(s) 173, 240
BmsI GCATC 2 cut(s) 196, 466
BmtI GCTAGC 1 cut(s) 69
BplI GAGNNNNNCTC 2 cut(s) 740, 772
Bpu10I CCTNAGC 1 cut(s) 631
BsaBI GATNNNNATC 1 cut(s) 495
BsaJI CCNNGG 3 cut(s) 171, 580, 643
Bsc4I CCNNNNNNNGG 1 cut(s) 127
Bse8I GATNNNNATC 1 cut(s) 495
BseBI CCWGG 2 cut(s) 173, 240
BseDI CCNNGG 3 cut(s) 171, 580, 643
BseGI GGATG 3 cut(s) 298, 407, 481
BseJI GATNNNNATC 1 cut(s) 495
BseLI CCNNNNNNNGG 1 cut(s) 127
BseMII CTCAG 6 cut(s) 270, 294, 514, 577, 645, 705
BseRI GAGGAG 1 cut(s) 761
BseYI CCCAGC 2 cut(s) 323, 653
BshFI GGCC 2 cut(s) 243, 579
BslFI GGGAC 2 cut(s) 27, 670
BslI CCNNNNNNNGG 1 cut(s) 127
BsmAI GTCTC 1 cut(s) 794
BsmFI GGGAC 2 cut(s) 27, 670
BsmI GAATGC 1 cut(s) 780
BsnI GGCC 2 cut(s) 243, 579
Bsp1407I TGTACA 1 cut(s) 133
Bsp143I GATC 6 cut(s) 210, 376, 490, 496, 684, 811
BspANI GGCC 2 cut(s) 243, 579
BspCNI CTCAG 6 cut(s) 269, 295, 513, 576, 644, 704
BspLI GGNNCC 2 cut(s) 197, 329
BspOI GCTAGC 1 cut(s) 69
BspPI GGATC 1 cut(s) 485
BspQI GCTCTTC 1 cut(s) 218
BsrGI TGTACA 1 cut(s) 133
BssECI CCNNGG 3 cut(s) 171, 580, 643
BssMI GATC 6 cut(s) 210, 376, 490, 496, 684, 811
BssT1I CCWWGG 2 cut(s) 580, 643
Bst2UI CCWGG 2 cut(s) 173, 240
Bst4CI ACNGT 3 cut(s) 138, 452, 661
Bst6I CTCTTC 3 cut(s) 39, 201, 218
BstAUI TGTACA 1 cut(s) 133
BstC8I GCNNGC 3 cut(s) 67, 458, 519
BstDEI CTNAG 7 cut(s) 256, 303, 500, 563, 600, 631, 691
BstENI CCTNNNNNAGG 1 cut(s) 125
BstF5I GGATG 3 cut(s) 298, 407, 481
BstKTI GATC 6 cut(s) 213, 379, 493, 499, 687, 814
BstMAI GTCTC 1 cut(s) 794
BstMBI GATC 6 cut(s) 210, 376, 490, 496, 684, 811
BstMWI GCNNNNNNNGC 2 cut(s) 147, 270
BstNI CCWGG 2 cut(s) 173, 240
BstSCI CCNGG 2 cut(s) 171, 238
BstSFI CTRYAG 2 cut(s) 177, 827
BstX2I RGATCY 1 cut(s) 496
BstYI RGATCY 1 cut(s) 496
BsuRI GGCC 2 cut(s) 243, 579
BtsCI GGATG 3 cut(s) 298, 407, 481
BtsI GCAGTG 1 cut(s) 625
BtsIMutI CAGTG 2 cut(s) 143, 625
Cac8I GCNNGC 3 cut(s) 67, 458, 519
Cfr13I GGNCC 2 cut(s) 196, 678
Csp6I GTAC 1 cut(s) 134
CviAII CATG 1 cut(s) 432
CviQI GTAC 1 cut(s) 134
DdeI CTNAG 7 cut(s) 256, 303, 500, 563, 600, 631, 691
DpnI GATC 6 cut(s) 212, 378, 492, 498, 686, 813
DpnII GATC 6 cut(s) 210, 376, 490, 496, 684, 811
EaeI YGGCCR 1 cut(s) 241
Eam1104I CTCTTC 3 cut(s) 39, 201, 218
EarI CTCTTC 3 cut(s) 39, 201, 218
Eco130I CCWWGG 2 cut(s) 580, 643
Eco47I GGWCC 2 cut(s) 196, 678
Eco57I CTGAAG 2 cut(s) 336, 558
EcoNI CCTNNNNNAGG 1 cut(s) 125
EcoRI GAATTC 1 cut(s) 88
EcoRII CCWGG 2 cut(s) 171, 238
EcoT14I CCWWGG 2 cut(s) 580, 643
ErhI CCWWGG 2 cut(s) 580, 643
FaeI CATG 1 cut(s) 435
FaiI YATR 6 cut(s) 8, 395, 404, 433, 720, 783
FalI AAGNNNNNCTT 2 cut(s) 18, 50
FaqI GGGAC 2 cut(s) 27, 670
FatI CATG 1 cut(s) 431
FbaI TGATCA 1 cut(s) 376
FokI GGATG 3 cut(s) 285, 394, 488
FspBI CTAG 2 cut(s) 66, 270
GsaI CCCAGC 2 cut(s) 327, 657
HaeIII GGCC 2 cut(s) 243, 579
Hin1II CATG 1 cut(s) 435
HindIII AAGCTT 1 cut(s) 458
HphI GGTGA 3 cut(s) 280, 596, 647
Hpy166II GTNNAC 1 cut(s) 134
Hpy188I TCNGA 5 cut(s) 259, 316, 418, 441, 694
Hpy188III TCNNGA 5 cut(s) 203, 302, 494, 542, 771
Hpy8I GTNNAC 1 cut(s) 134
HpyAV CCTTC 5 cut(s) 311, 423, 474, 532, 788
HpyCH4III ACNGT 3 cut(s) 138, 452, 661
HpyCH4V TGCA 2 cut(s) 84, 558
HpyF10VI GCNNNNNNNGC 2 cut(s) 147, 270
HpyF3I CTNAG 7 cut(s) 256, 303, 500, 563, 600, 631, 691
Hsp92II CATG 1 cut(s) 435
Ksp22I TGATCA 1 cut(s) 376
Kzo9I GATC 6 cut(s) 210, 376, 490, 496, 684, 811
LguI GCTCTTC 1 cut(s) 218
LmnI GCTCC 4 cut(s) 22, 64, 327, 529
LweI GCATC 2 cut(s) 196, 466
MaeI CTAG 2 cut(s) 66, 270
MaeIII GTNAC 4 cut(s) 251, 265, 481, 533
MalI GATC 6 cut(s) 212, 378, 492, 498, 686, 813
MboI GATC 6 cut(s) 210, 376, 490, 496, 684, 811
MboII GAAGA 8 cut(s) 26, 69, 182, 218, 235, 564, 725, 803
MflI RGATCY 1 cut(s) 496
MlsI TGGCCA 1 cut(s) 243
MluCI AATT 4 cut(s) 88, 388, 553, 760
MluNI TGGCCA 1 cut(s) 243
MnlI CCTC 9 cut(s) 8, 48, 55, 294, 608, 615, 726, 739, 742
Mox20I TGGCCA 1 cut(s) 243
MscI TGGCCA 1 cut(s) 243
MseI TTAA 3 cut(s) 96, 552, 741
Msp20I TGGCCA 1 cut(s) 243
MspR9I CCNGG 2 cut(s) 173, 240
Mva1269I GAATGC 1 cut(s) 780
MvaI CCWGG 2 cut(s) 173, 240
MwoI GCNNNNNNNGC 2 cut(s) 147, 270
NdeII GATC 6 cut(s) 210, 376, 490, 496, 684, 811
NheI GCTAGC 1 cut(s) 65
NlaIII CATG 1 cut(s) 435
NlaIV GGNNCC 2 cut(s) 197, 329
PciSI GCTCTTC 1 cut(s) 218
PctI GAATGC 1 cut(s) 780
Psp6I CCWGG 2 cut(s) 171, 238
PspFI CCCAGC 2 cut(s) 323, 653
PspGI CCWGG 2 cut(s) 171, 238
PspN4I GGNNCC 2 cut(s) 197, 329
PspPI GGNCC 2 cut(s) 196, 678
PsuI RGATCY 1 cut(s) 496
RsaI GTAC 1 cut(s) 135
RsaNI GTAC 1 cut(s) 134
SapI GCTCTTC 1 cut(s) 218
SaqAI TTAA 3 cut(s) 96, 552, 741
Sau3AI GATC 6 cut(s) 210, 376, 490, 496, 684, 811
Sau96I GGNCC 2 cut(s) 196, 678
ScrFI CCNGG 2 cut(s) 173, 240
SfaNI GCATC 2 cut(s) 196, 466
SfcI CTRYAG 2 cut(s) 177, 827
SinI GGWCC 2 cut(s) 196, 678
Sse9I AATT 4 cut(s) 88, 388, 553, 760
SspMI CTAG 2 cut(s) 66, 270
StyD4I CCNGG 2 cut(s) 171, 238
StyI CCWWGG 2 cut(s) 580, 643
TaaI ACNGT 3 cut(s) 138, 452, 661
TasI AATT 4 cut(s) 88, 388, 553, 760
TatI WGTACW 1 cut(s) 133
Tru1I TTAA 3 cut(s) 96, 552, 741
Tru9I TTAA 3 cut(s) 96, 552, 741
TscAI CASTG 2 cut(s) 143, 632
TspDTI ATGAA 1 cut(s) 236
TspRI CASTG 2 cut(s) 143, 632
VpaK11BI GGWCC 2 cut(s) 196, 678
XagI CCTNNNNNAGG 1 cut(s) 125
XapI RAATTY 2 cut(s) 88, 388
XcmI CCANNNNNNNNNTGG 2 cut(s) 587, 650
XspI CTAG 2 cut(s) 66, 270
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.