Rh5AG111300

zinc finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0017750
Physical Location & Seq
Reverse (-)
10253466 .. 10256364
2899 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG111300.1

Sequence Viewer

Length: 729 bp
ATGTGGTGCAACTGGTCACATAAACCTGGTGTACAGTGCTTCTATTGCCAAAGCGTGTTCAACAATCCCCAGGCTGTAGGGGGGCATCTTTGGGTCCATCAGGAAGAGATCAAGGAAATGAAGAGCTGGAACTTTCCTGGCCATTTCAGCAACTCAGAAACCGTTACTAGCAATACTCAACCTCCCACCATCTCCCATCCTGAGAACTTTTCTGAAGGTGCTGGGAGCCATCAATCTCGTTTGCTCTCAAATGGAGTTTGTCAGTTCAATACTGATCAAGTTCAAAATTTTATGGATAGTATGCCATCCCTTTCTGAAAATGCCTTGCCATGCTTTCAGAATAACTACTGTGGCAAGCTTCCTTCTCTTGTGGATGCTGTAACTTTTGATCCAGATCTCAGCTCAAATCAGTCGCCTGCCTTGAAGGAGCGTAATATTCTTGATTTCTTTAATTGCAATCTCAGTCAATCTTCAGGCCAAGGTGAAGCTGGTCAATCTAAGAAAAATAATGGAGGTAAGAGGCGCTGCTCAGGTGAAGCCCAAGGAAATGCTGGGACTGTGAATGAGTTGAAAAAGACCAAGATCAACTCAGATGAGATGGAAACTGATAGTCCTCAGAAGAGAGAGCTTCTTCTTGTTAAAGAGGAGGACAACTCCAATTCTGGTTCAGGAATGCCTTATGATGTGGAAGAAGGCGAGACAAACCTTGATCTGTCGCTACATCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

242

Amino Acids

26.76

Weight (kDa)

4.97

Isoelectric Point (pI)

47.93

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017750)

Species Orthologous Gene IDs
prunus_persica Prupe.4G081800_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0014311
rosa_laevigata RLG00000032097
rosa_multiflora Rmu_sc0009295.1_g000004
rosa_roxburghii Rroxscaffold_1G00062270
rosa_rugosa Rorug05G0017100 Rorug05G0017200 Rorug05G0017300
rosa_samantha Rh5AG111300 Rh5BG108600 Rh5CG120300 Rh5DG107300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 383
AcoI YGGCCR 1 cut(s) 139
AcsI RAATTY 1 cut(s) 286
AcuI CTGAAG 2 cut(s) 234, 456
AfaI GTAC 1 cut(s) 33
AgsI TTSAA 5 cut(s) 61, 268, 284, 424, 571
AjnI CCWGG 3 cut(s) 25, 69, 136
AluBI AGCT 5 cut(s) 126, 358, 402, 488, 628
AluI AGCT 5 cut(s) 126, 358, 402, 488, 628
Alw26I GTCTC 1 cut(s) 692
AlwI GGATC 1 cut(s) 383
AoxI GGCC 2 cut(s) 139, 475
ApeKI GCWGC 1 cut(s) 525
ApoI RAATTY 1 cut(s) 286
AspLEI GCGC 1 cut(s) 525
AspS9I GGNCC 1 cut(s) 94
AsuHPI GGTGA 2 cut(s) 494, 545
AvaII GGWCC 1 cut(s) 94
BalI TGGCCA 1 cut(s) 141
BbvI GCAGC 1 cut(s) 512
BccI CCATC 6 cut(s) 105, 197, 204, 237, 313, 592
BciT130I CCWGG 3 cut(s) 27, 71, 138
BclI TGATCA 1 cut(s) 274
BcoDI GTCTC 1 cut(s) 692
BfaI CTAG 1 cut(s) 168
BfmI CTRYAG 1 cut(s) 75
BfoI RGCGCY 1 cut(s) 526
BglII AGATCT 1 cut(s) 394
BisI GCNGC 1 cut(s) 526
BlsI GCNGC 1 cut(s) 527
Bme1390I CCNGG 3 cut(s) 27, 71, 138
Bme18I GGWCC 1 cut(s) 94
BmgT120I GGNCC 1 cut(s) 94
BmiI GGNNCC 2 cut(s) 95, 227
BmrFI CCNGG 3 cut(s) 27, 71, 138
BmsI GCATC 2 cut(s) 94, 364
BplI GAGNNNNNCTC 2 cut(s) 638, 670
Bpu10I CCTNAGC 1 cut(s) 529
BsaBI GATNNNNATC 1 cut(s) 393
BsaJI CCNNGG 3 cut(s) 69, 478, 541
BsaXI ACNNNNNCTCC 2 cut(s) 166, 196
Bse1I ACTGG 1 cut(s) 17
Bse8I GATNNNNATC 1 cut(s) 393
BseBI CCWGG 3 cut(s) 27, 71, 138
BseDI CCNNGG 3 cut(s) 69, 478, 541
BseGI GGATG 3 cut(s) 196, 305, 379
BseJI GATNNNNATC 1 cut(s) 393
BseMII CTCAG 7 cut(s) 168, 192, 412, 475, 543, 603, 629
BseNI ACTGG 1 cut(s) 17
BseRI GAGGAG 1 cut(s) 659
BseXI GCAGC 1 cut(s) 512
BseYI CCCAGC 2 cut(s) 221, 551
BshFI GGCC 2 cut(s) 141, 477
BslFI GGGAC 1 cut(s) 568
BsmAI GTCTC 1 cut(s) 692
BsmFI GGGAC 1 cut(s) 568
BsmI GAATGC 1 cut(s) 678
BsnI GGCC 2 cut(s) 141, 477
Bsp1407I TGTACA 1 cut(s) 31
Bsp143I GATC 6 cut(s) 108, 274, 388, 394, 582, 709
BspANI GGCC 2 cut(s) 141, 477
BspCNI CTCAG 7 cut(s) 167, 193, 411, 474, 542, 602, 628
BspLI GGNNCC 2 cut(s) 95, 227
BspPI GGATC 1 cut(s) 383
BspQI GCTCTTC 1 cut(s) 116
BsrGI TGTACA 1 cut(s) 31
BsrI ACTGG 1 cut(s) 17
BssECI CCNNGG 3 cut(s) 69, 478, 541
BssMI GATC 6 cut(s) 108, 274, 388, 394, 582, 709
BssT1I CCWWGG 2 cut(s) 478, 541
Bst2UI CCWGG 3 cut(s) 27, 71, 138
Bst4CI ACNGT 4 cut(s) 36, 163, 350, 559
Bst6I CTCTTC 3 cut(s) 99, 116, 614
BstAUI TGTACA 1 cut(s) 31
BstC8I GCNNGC 2 cut(s) 356, 417
BstDEI CTNAG 8 cut(s) 154, 201, 398, 461, 498, 529, 589, 615
BstF5I GGATG 3 cut(s) 196, 305, 379
BstH2I RGCGCY 1 cut(s) 526
BstHHI GCGC 1 cut(s) 525
BstKTI GATC 6 cut(s) 111, 277, 391, 397, 585, 712
BstMAI GTCTC 1 cut(s) 692
BstMBI GATC 6 cut(s) 108, 274, 388, 394, 582, 709
BstMWI GCNNNNNNNGC 2 cut(s) 45, 147
BstNI CCWGG 3 cut(s) 27, 71, 138
BstSCI CCNGG 3 cut(s) 25, 69, 136
BstSFI CTRYAG 1 cut(s) 75
BstV1I GCAGC 1 cut(s) 512
BstX2I RGATCY 1 cut(s) 394
BstYI RGATCY 1 cut(s) 394
BsuRI GGCC 2 cut(s) 141, 477
BtsCI GGATG 3 cut(s) 196, 305, 379
BtsIMutI CAGTG 1 cut(s) 41
Cac8I GCNNGC 2 cut(s) 356, 417
CfoI GCGC 1 cut(s) 525
Cfr13I GGNCC 1 cut(s) 94
CsiI ACCWGGT 1 cut(s) 25
Csp6I GTAC 1 cut(s) 32
CviAII CATG 1 cut(s) 330
CviQI GTAC 1 cut(s) 32
DdeI CTNAG 8 cut(s) 154, 201, 398, 461, 498, 529, 589, 615
DpnI GATC 6 cut(s) 110, 276, 390, 396, 584, 711
DpnII GATC 6 cut(s) 108, 274, 388, 394, 582, 709
EaeI YGGCCR 1 cut(s) 139
Eam1104I CTCTTC 3 cut(s) 99, 116, 614
EarI CTCTTC 3 cut(s) 99, 116, 614
Eco130I CCWWGG 2 cut(s) 478, 541
Eco47I GGWCC 1 cut(s) 94
Eco57I CTGAAG 2 cut(s) 234, 456
EcoRII CCWGG 3 cut(s) 25, 69, 136
EcoT14I CCWWGG 2 cut(s) 478, 541
ErhI CCWWGG 2 cut(s) 478, 541
FaeI CATG 1 cut(s) 333
FaiI YATR 5 cut(s) 21, 293, 302, 331, 681
FaqI GGGAC 1 cut(s) 568
FatI CATG 1 cut(s) 329
FbaI TGATCA 1 cut(s) 274
Fnu4HI GCNGC 1 cut(s) 526
FokI GGATG 3 cut(s) 183, 292, 386
Fsp4HI GCNGC 1 cut(s) 526
FspBI CTAG 1 cut(s) 168
GlaI GCGC 1 cut(s) 524
GluI GCNGC 1 cut(s) 526
GsaI CCCAGC 2 cut(s) 225, 555
HaeII RGCGCY 1 cut(s) 526
HaeIII GGCC 2 cut(s) 141, 477
HhaI GCGC 1 cut(s) 525
Hin1II CATG 1 cut(s) 333
Hin6I GCGC 1 cut(s) 523
HinP1I GCGC 1 cut(s) 523
HindIII AAGCTT 1 cut(s) 356
HphI GGTGA 2 cut(s) 494, 545
Hpy166II GTNNAC 1 cut(s) 32
Hpy188I TCNGA 6 cut(s) 157, 214, 316, 339, 592, 618
Hpy188III TCNNGA 5 cut(s) 101, 200, 392, 440, 669
Hpy8I GTNNAC 1 cut(s) 32
HpyAV CCTTC 4 cut(s) 209, 372, 418, 686
HpyCH4III ACNGT 4 cut(s) 36, 163, 350, 559
HpyCH4V TGCA 2 cut(s) 9, 456
HpyF10VI GCNNNNNNNGC 2 cut(s) 45, 147
HpyF3I CTNAG 8 cut(s) 154, 201, 398, 461, 498, 529, 589, 615
Hsp92II CATG 1 cut(s) 333
HspAI GCGC 1 cut(s) 523
Ksp22I TGATCA 1 cut(s) 274
Kzo9I GATC 6 cut(s) 108, 274, 388, 394, 582, 709
LguI GCTCTTC 1 cut(s) 116
LmnI GCTCC 2 cut(s) 225, 427
Lsp1109I GCAGC 1 cut(s) 512
LweI GCATC 2 cut(s) 94, 364
MabI ACCWGGT 1 cut(s) 25
MaeI CTAG 1 cut(s) 168
MaeIII GTNAC 3 cut(s) 15, 163, 379
MalI GATC 6 cut(s) 110, 276, 390, 396, 584, 711
MboI GATC 6 cut(s) 108, 274, 388, 394, 582, 709
MboII GAAGA 6 cut(s) 116, 133, 462, 623, 631, 701
MflI RGATCY 1 cut(s) 394
MlsI TGGCCA 1 cut(s) 141
MluCI AATT 3 cut(s) 286, 451, 658
MluNI TGGCCA 1 cut(s) 141
MnlI CCTC 6 cut(s) 192, 506, 513, 624, 637, 640
Mox20I TGGCCA 1 cut(s) 141
MscI TGGCCA 1 cut(s) 141
MseI TTAA 2 cut(s) 450, 639
Msp20I TGGCCA 1 cut(s) 141
MspR9I CCNGG 3 cut(s) 27, 71, 138
Mva1269I GAATGC 1 cut(s) 678
MvaI CCWGG 3 cut(s) 27, 71, 138
MwoI GCNNNNNNNGC 2 cut(s) 45, 147
NdeII GATC 6 cut(s) 108, 274, 388, 394, 582, 709
NlaIII CATG 1 cut(s) 333
NlaIV GGNNCC 2 cut(s) 95, 227
NmuCI GTSAC 1 cut(s) 15
PciSI GCTCTTC 1 cut(s) 116
PctI GAATGC 1 cut(s) 678
PkrI GCNGC 1 cut(s) 527
Psp6I CCWGG 3 cut(s) 25, 69, 136
PspFI CCCAGC 2 cut(s) 221, 551
PspGI CCWGG 3 cut(s) 25, 69, 136
PspN4I GGNNCC 2 cut(s) 95, 227
PspPI GGNCC 1 cut(s) 94
PsuI RGATCY 1 cut(s) 394
RsaI GTAC 1 cut(s) 33
RsaNI GTAC 1 cut(s) 32
SapI GCTCTTC 1 cut(s) 116
SaqAI TTAA 2 cut(s) 450, 639
SatI GCNGC 1 cut(s) 526
Sau3AI GATC 6 cut(s) 108, 274, 388, 394, 582, 709
Sau96I GGNCC 1 cut(s) 94
ScrFI CCNGG 3 cut(s) 27, 71, 138
SexAI ACCWGGT 1 cut(s) 25
SfaNI GCATC 2 cut(s) 94, 364
SfcI CTRYAG 1 cut(s) 75
SinI GGWCC 1 cut(s) 94
Sse9I AATT 3 cut(s) 286, 451, 658
SspI AATATT 1 cut(s) 436
SspMI CTAG 1 cut(s) 168
StyD4I CCNGG 3 cut(s) 25, 69, 136
StyI CCWWGG 2 cut(s) 478, 541
TaaI ACNGT 4 cut(s) 36, 163, 350, 559
TasI AATT 3 cut(s) 286, 451, 658
TatI WGTACW 1 cut(s) 31
Tru1I TTAA 2 cut(s) 450, 639
Tru9I TTAA 2 cut(s) 450, 639
TscAI CASTG 1 cut(s) 41
TseFI GTSAC 1 cut(s) 15
TseI GCWGC 1 cut(s) 525
Tsp45I GTSAC 1 cut(s) 15
TspDTI ATGAA 1 cut(s) 134
TspRI CASTG 1 cut(s) 41
VpaK11BI GGWCC 1 cut(s) 94
XapI RAATTY 1 cut(s) 286
XcmI CCANNNNNNNNNTGG 2 cut(s) 485, 548
XspI CTAG 1 cut(s) 168
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.