Rh5CG120300

zinc finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0017750
Physical Location & Seq
Reverse (-)
9961714 .. 9966175
4462 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG120300.1

Sequence Viewer

Length: 729 bp
ATGTGGTGCAACTGGTCACATAAACCTGGTGTACAGTGCTTCTATTGCCAAAGCGTGTTCAACAATCCCCAGGCTGTAGAGGGGCATCTTTGGGTCCATCAGGAAGAGATCAAGGAAATGAAGAGCTGGAACTTTCCTGGCCATTTCAGCAACTCAGAAAGCGTTACTAGCAATACTCAACCTCTCACCATCTCCCATCCTGAGAACTTTTCTGAAGGTGCTGGGAGCCATCAATCTCATTTGCTCTCAAATGGAGTTTGTCAGTTCAATACTGATCAAGTTCAAAATTTTATGGATAGTATGCCATCCCTTTCTGAAAATGCCTTGCCATGCTTTCAGAATAACTACTGTGGCAAGCTTCCTTCTCTTGTGGATGCTGTAACTTTTGATCCAGATCTCAGCTCAAATCAGTCGCCTGCCTTCAAGGAGCGTAATATTCTTGATTTCTTTAATTGCAATCTCAGTCAATCTTCAGGCCAAGGTGAAGCTGGTCAATCTAAGAAAAATAATGGAGGTAAGAGGCCCTGCTCAGGTGAAGCCCAAGGAAATGCTGGGACTGTGAATGAGTTGAAAAGGACCAAGATCAACTCAGATGAGATGGAAACTGATAGTCCTCAGAAGAGAGAGCTTCTTCTTGTTAAAGAGGAGGACAACTCCAATTCTGGTTCTGGAATGCCTTATGATGTGGAAGAAGGCGAGACAAACCTTGATCTGTCGCTACATCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

242

Amino Acids

26.81

Weight (kDa)

4.83

Isoelectric Point (pI)

46.17

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017750)

Species Orthologous Gene IDs
prunus_persica Prupe.4G081800_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0014311
rosa_laevigata RLG00000032097
rosa_multiflora Rmu_sc0009295.1_g000004
rosa_roxburghii Rroxscaffold_1G00062270
rosa_rugosa Rorug05G0017100 Rorug05G0017200 Rorug05G0017300
rosa_samantha Rh5AG111300 Rh5BG108600 Rh5CG120300 Rh5DG107300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 383
AcoI YGGCCR 1 cut(s) 139
AcsI RAATTY 1 cut(s) 286
AcuI CTGAAG 2 cut(s) 234, 456
AfaI GTAC 1 cut(s) 33
AfiI CCNNNNNNNGG 1 cut(s) 530
AgsI TTSAA 5 cut(s) 61, 268, 284, 424, 571
AjnI CCWGG 3 cut(s) 25, 69, 136
AluBI AGCT 5 cut(s) 126, 358, 402, 488, 628
AluI AGCT 5 cut(s) 126, 358, 402, 488, 628
Alw26I GTCTC 1 cut(s) 692
AlwI GGATC 1 cut(s) 383
AoxI GGCC 3 cut(s) 139, 475, 521
ApoI RAATTY 1 cut(s) 286
AspS9I GGNCC 3 cut(s) 94, 522, 576
AsuHPI GGTGA 3 cut(s) 178, 494, 545
AvaII GGWCC 2 cut(s) 94, 576
BalI TGGCCA 1 cut(s) 141
BccI CCATC 6 cut(s) 105, 197, 204, 237, 313, 592
BciT130I CCWGG 3 cut(s) 27, 71, 138
BclI TGATCA 1 cut(s) 274
BcoDI GTCTC 1 cut(s) 692
BfaI CTAG 1 cut(s) 168
BfmI CTRYAG 1 cut(s) 75
BglII AGATCT 1 cut(s) 394
Bme1390I CCNGG 3 cut(s) 27, 71, 138
Bme18I GGWCC 2 cut(s) 94, 576
BmgT120I GGNCC 3 cut(s) 94, 522, 576
BmiI GGNNCC 2 cut(s) 95, 227
BmrFI CCNGG 3 cut(s) 27, 71, 138
BmsI GCATC 2 cut(s) 94, 364
BplI GAGNNNNNCTC 2 cut(s) 638, 670
Bpu10I CCTNAGC 1 cut(s) 529
BsaBI GATNNNNATC 1 cut(s) 393
BsaJI CCNNGG 3 cut(s) 69, 478, 541
Bsc4I CCNNNNNNNGG 1 cut(s) 530
Bse1I ACTGG 1 cut(s) 17
Bse8I GATNNNNATC 1 cut(s) 393
BseBI CCWGG 3 cut(s) 27, 71, 138
BseDI CCNNGG 3 cut(s) 69, 478, 541
BseGI GGATG 3 cut(s) 196, 305, 379
BseJI GATNNNNATC 1 cut(s) 393
BseLI CCNNNNNNNGG 1 cut(s) 530
BseMII CTCAG 7 cut(s) 168, 192, 412, 475, 543, 603, 629
BseNI ACTGG 1 cut(s) 17
BseRI GAGGAG 1 cut(s) 659
BseYI CCCAGC 2 cut(s) 221, 551
BshFI GGCC 3 cut(s) 141, 477, 523
BslFI GGGAC 1 cut(s) 568
BslI CCNNNNNNNGG 1 cut(s) 530
BsmAI GTCTC 1 cut(s) 692
BsmFI GGGAC 1 cut(s) 568
BsmI GAATGC 1 cut(s) 678
BsnI GGCC 3 cut(s) 141, 477, 523
Bsp1407I TGTACA 1 cut(s) 31
Bsp143I GATC 6 cut(s) 108, 274, 388, 394, 582, 709
BspANI GGCC 3 cut(s) 141, 477, 523
BspCNI CTCAG 7 cut(s) 167, 193, 411, 474, 542, 602, 628
BspLI GGNNCC 2 cut(s) 95, 227
BspPI GGATC 1 cut(s) 383
BspQI GCTCTTC 1 cut(s) 116
BsrGI TGTACA 1 cut(s) 31
BsrI ACTGG 1 cut(s) 17
BssECI CCNNGG 3 cut(s) 69, 478, 541
BssMI GATC 6 cut(s) 108, 274, 388, 394, 582, 709
BssT1I CCWWGG 2 cut(s) 478, 541
Bst2UI CCWGG 3 cut(s) 27, 71, 138
Bst4CI ACNGT 3 cut(s) 36, 350, 559
Bst6I CTCTTC 3 cut(s) 99, 116, 614
BstAUI TGTACA 1 cut(s) 31
BstC8I GCNNGC 2 cut(s) 356, 417
BstDEI CTNAG 8 cut(s) 154, 201, 398, 461, 498, 529, 589, 615
BstF5I GGATG 3 cut(s) 196, 305, 379
BstKTI GATC 6 cut(s) 111, 277, 391, 397, 585, 712
BstMAI GTCTC 1 cut(s) 692
BstMBI GATC 6 cut(s) 108, 274, 388, 394, 582, 709
BstMWI GCNNNNNNNGC 3 cut(s) 45, 147, 168
BstNI CCWGG 3 cut(s) 27, 71, 138
BstSCI CCNGG 3 cut(s) 25, 69, 136
BstSFI CTRYAG 1 cut(s) 75
BstX2I RGATCY 1 cut(s) 394
BstYI RGATCY 1 cut(s) 394
BsuRI GGCC 3 cut(s) 141, 477, 523
BtsCI GGATG 3 cut(s) 196, 305, 379
BtsIMutI CAGTG 1 cut(s) 41
Cac8I GCNNGC 2 cut(s) 356, 417
Cfr13I GGNCC 3 cut(s) 94, 522, 576
CsiI ACCWGGT 1 cut(s) 25
Csp6I GTAC 1 cut(s) 32
CviAII CATG 1 cut(s) 330
CviQI GTAC 1 cut(s) 32
DdeI CTNAG 8 cut(s) 154, 201, 398, 461, 498, 529, 589, 615
DpnI GATC 6 cut(s) 110, 276, 390, 396, 584, 711
DpnII GATC 6 cut(s) 108, 274, 388, 394, 582, 709
EaeI YGGCCR 1 cut(s) 139
Eam1104I CTCTTC 3 cut(s) 99, 116, 614
EarI CTCTTC 3 cut(s) 99, 116, 614
Eco130I CCWWGG 2 cut(s) 478, 541
Eco47I GGWCC 2 cut(s) 94, 576
Eco57I CTGAAG 2 cut(s) 234, 456
EcoO109I RGGNCCY 1 cut(s) 522
EcoRII CCWGG 3 cut(s) 25, 69, 136
EcoT14I CCWWGG 2 cut(s) 478, 541
ErhI CCWWGG 2 cut(s) 478, 541
FaeI CATG 1 cut(s) 333
FaiI YATR 5 cut(s) 21, 293, 302, 331, 681
FaqI GGGAC 1 cut(s) 568
FatI CATG 1 cut(s) 329
FbaI TGATCA 1 cut(s) 274
FokI GGATG 3 cut(s) 183, 292, 386
FspBI CTAG 1 cut(s) 168
GsaI CCCAGC 2 cut(s) 225, 555
HaeIII GGCC 3 cut(s) 141, 477, 523
Hin1II CATG 1 cut(s) 333
HindIII AAGCTT 1 cut(s) 356
HphI GGTGA 3 cut(s) 178, 494, 545
Hpy166II GTNNAC 1 cut(s) 32
Hpy188I TCNGA 6 cut(s) 157, 214, 316, 339, 592, 618
Hpy188III TCNNGA 5 cut(s) 101, 200, 392, 440, 669
Hpy8I GTNNAC 1 cut(s) 32
HpyAV CCTTC 4 cut(s) 209, 372, 430, 686
HpyCH4III ACNGT 3 cut(s) 36, 350, 559
HpyCH4V TGCA 2 cut(s) 9, 456
HpyF10VI GCNNNNNNNGC 3 cut(s) 45, 147, 168
HpyF3I CTNAG 8 cut(s) 154, 201, 398, 461, 498, 529, 589, 615
Hsp92II CATG 1 cut(s) 333
Ksp22I TGATCA 1 cut(s) 274
Kzo9I GATC 6 cut(s) 108, 274, 388, 394, 582, 709
LguI GCTCTTC 1 cut(s) 116
LmnI GCTCC 2 cut(s) 225, 427
LweI GCATC 2 cut(s) 94, 364
MabI ACCWGGT 1 cut(s) 25
MaeI CTAG 1 cut(s) 168
MaeIII GTNAC 3 cut(s) 15, 163, 379
MalI GATC 6 cut(s) 110, 276, 390, 396, 584, 711
MboI GATC 6 cut(s) 108, 274, 388, 394, 582, 709
MboII GAAGA 6 cut(s) 116, 133, 462, 623, 631, 701
MflI RGATCY 1 cut(s) 394
MlsI TGGCCA 1 cut(s) 141
MluCI AATT 3 cut(s) 286, 451, 658
MluNI TGGCCA 1 cut(s) 141
MnlI CCTC 7 cut(s) 73, 192, 506, 513, 624, 637, 640
Mox20I TGGCCA 1 cut(s) 141
MscI TGGCCA 1 cut(s) 141
MseI TTAA 2 cut(s) 450, 639
Msp20I TGGCCA 1 cut(s) 141
MspR9I CCNGG 3 cut(s) 27, 71, 138
Mva1269I GAATGC 1 cut(s) 678
MvaI CCWGG 3 cut(s) 27, 71, 138
MwoI GCNNNNNNNGC 3 cut(s) 45, 147, 168
NdeII GATC 6 cut(s) 108, 274, 388, 394, 582, 709
NlaIII CATG 1 cut(s) 333
NlaIV GGNNCC 2 cut(s) 95, 227
NmuCI GTSAC 1 cut(s) 15
PciSI GCTCTTC 1 cut(s) 116
PctI GAATGC 1 cut(s) 678
Psp6I CCWGG 3 cut(s) 25, 69, 136
PspFI CCCAGC 2 cut(s) 221, 551
PspGI CCWGG 3 cut(s) 25, 69, 136
PspN4I GGNNCC 2 cut(s) 95, 227
PspPI GGNCC 3 cut(s) 94, 522, 576
PsuI RGATCY 1 cut(s) 394
RsaI GTAC 1 cut(s) 33
RsaNI GTAC 1 cut(s) 32
SapI GCTCTTC 1 cut(s) 116
SaqAI TTAA 2 cut(s) 450, 639
Sau3AI GATC 6 cut(s) 108, 274, 388, 394, 582, 709
Sau96I GGNCC 3 cut(s) 94, 522, 576
ScrFI CCNGG 3 cut(s) 27, 71, 138
SexAI ACCWGGT 1 cut(s) 25
SfaNI GCATC 2 cut(s) 94, 364
SfcI CTRYAG 1 cut(s) 75
SinI GGWCC 2 cut(s) 94, 576
Sse9I AATT 3 cut(s) 286, 451, 658
SspI AATATT 1 cut(s) 436
SspMI CTAG 1 cut(s) 168
StyD4I CCNGG 3 cut(s) 25, 69, 136
StyI CCWWGG 2 cut(s) 478, 541
TaaI ACNGT 3 cut(s) 36, 350, 559
TasI AATT 3 cut(s) 286, 451, 658
TatI WGTACW 1 cut(s) 31
Tru1I TTAA 2 cut(s) 450, 639
Tru9I TTAA 2 cut(s) 450, 639
TscAI CASTG 1 cut(s) 41
TseFI GTSAC 1 cut(s) 15
Tsp45I GTSAC 1 cut(s) 15
TspDTI ATGAA 1 cut(s) 134
TspRI CASTG 1 cut(s) 41
VpaK11BI GGWCC 2 cut(s) 94, 576
XapI RAATTY 1 cut(s) 286
XcmI CCANNNNNNNNNTGG 2 cut(s) 485, 548
XspI CTAG 1 cut(s) 168
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.