Rmu_sc0016399.1_g000001

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0016399.1
OG0018258
Physical Location & Seq
Reverse (-)
13976 .. 15693
1718 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0016399.1_g000001.1.cds

Sequence Viewer

Length: 375 bp
atgccacttgttgcagcaaccaaaaactttgggactactagtgtaccagttgctgcagcaatagctctcaggaacagtcttgttttggccaagcggaaaggatatactaatatacatgcagaaggagactccaagttagtcattgatgctgtggaaggaagaaaaggggaggatgcggtgggtgatgaaggagaggtagggggtgcacttgagccagaggcggcggagcaagtgtgggctgcaaatggaggacgcaagatggagataggcccaggcgacttgcttgacgaggcggttatggatggagatctcgtagccggagttggtggtcaagttgggcgtcggggactggatggagatgaaagaggaggatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

124

Amino Acids

12.58

Weight (kDa)

4.58

Isoelectric Point (pI)

30.5

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 5 cut(s) 94, 176, 221, 224, 293
AcoI YGGCCR 1 cut(s) 87
AcyI GRCGYC 1 cut(s) 340
AfaI GTAC 1 cut(s) 45
AhlI ACTAGT 1 cut(s) 38
AjnI CCWGG 1 cut(s) 271
AluBI AGCT 1 cut(s) 65
AluI AGCT 1 cut(s) 65
Alw21I GWGCWC 1 cut(s) 208
Alw26I GTCTC 1 cut(s) 120
Alw44I GTGCAC 1 cut(s) 204
AlwNI CAGNNNCTG 1 cut(s) 53
AoxI GGCC 2 cut(s) 87, 268
ApaLI GTGCAC 1 cut(s) 204
ApeKI GCWGC 4 cut(s) 14, 53, 56, 239
AspS9I GGNCC 1 cut(s) 269
AsuHPI GGTGA 1 cut(s) 194
BaeGI GKGCMC 1 cut(s) 208
BaeI ACNNNNGTAYC 2 cut(s) 27, 60
BalI TGGCCA 1 cut(s) 89
Bbv12I GWGCWC 1 cut(s) 208
BbvI GCAGC 4 cut(s) 26, 40, 68, 226
BccI CCATC 3 cut(s) 253, 296, 347
BciT130I CCWGG 1 cut(s) 273
BcoDI GTCTC 1 cut(s) 120
BcuI ACTAGT 1 cut(s) 38
BfaI CTAG 1 cut(s) 39
BfmI CTRYAG 1 cut(s) 54
BglII AGATCT 1 cut(s) 307
BisI GCNGC 5 cut(s) 15, 54, 57, 222, 240
BlsI GCNGC 5 cut(s) 16, 55, 58, 223, 241
Bme1390I CCNGG 1 cut(s) 273
BmgT120I GGNCC 1 cut(s) 269
BmrFI CCNGG 1 cut(s) 273
BmsI GCATC 2 cut(s) 136, 163
BpuEI CTTGAG 1 cut(s) 230
BsaBI GATNNNNATC 1 cut(s) 306
BsaHI GRCGYC 1 cut(s) 340
BsaJI CCNNGG 1 cut(s) 271
BsaXI ACNNNNNCTCC 4 cut(s) 218, 248, 297, 327
Bse1I ACTGG 2 cut(s) 47, 354
Bse8I GATNNNNATC 1 cut(s) 306
BseBI CCWGG 1 cut(s) 273
BseDI CCNNGG 1 cut(s) 271
BseGI GGATG 3 cut(s) 178, 307, 358
BseJI GATNNNNATC 1 cut(s) 306
BseMII CTCAG 1 cut(s) 82
BseNI ACTGG 2 cut(s) 47, 354
BseSI GKGCMC 1 cut(s) 208
BseXI GCAGC 4 cut(s) 26, 40, 68, 226
BshFI GGCC 2 cut(s) 89, 270
BsiHKAI GWGCWC 1 cut(s) 208
BsiSI CCGG 1 cut(s) 318
BslFI GGGAC 2 cut(s) 46, 360
BsmAI GTCTC 1 cut(s) 120
BsmFI GGGAC 2 cut(s) 46, 360
BsnI GGCC 2 cut(s) 89, 270
Bsp1286I GDGCHC 1 cut(s) 208
Bsp143I GATC 1 cut(s) 307
BspACI CCGC 5 cut(s) 94, 176, 221, 224, 293
BspANI GGCC 2 cut(s) 89, 270
BspCNI CTCAG 1 cut(s) 81
BspMAI CTGCAG 1 cut(s) 58
BsrI ACTGG 2 cut(s) 47, 354
BssECI CCNNGG 1 cut(s) 271
BssMI GATC 1 cut(s) 307
BssNI GRCGYC 1 cut(s) 340
Bst2UI CCWGG 1 cut(s) 273
Bst4CI ACNGT 1 cut(s) 77
BstACI GRCGYC 1 cut(s) 340
BstDEI CTNAG 1 cut(s) 68
BstF5I GGATG 3 cut(s) 178, 307, 358
BstKTI GATC 1 cut(s) 310
BstMAI GTCTC 1 cut(s) 120
BstMBI GATC 1 cut(s) 307
BstMWI GCNNNNNNNGC 1 cut(s) 62
BstNI CCWGG 1 cut(s) 273
BstNSI RCATGY 1 cut(s) 119
BstSCI CCNGG 1 cut(s) 271
BstSFI CTRYAG 1 cut(s) 54
BstSLI GKGCMC 1 cut(s) 208
BstV1I GCAGC 4 cut(s) 26, 40, 68, 226
BstX2I RGATCY 1 cut(s) 307
BstYI RGATCY 1 cut(s) 307
BsuRI GGCC 2 cut(s) 89, 270
BtsCI GGATG 3 cut(s) 178, 307, 358
CaiI CAGNNNCTG 1 cut(s) 53
Cfr13I GGNCC 1 cut(s) 269
CseI GACGC 2 cut(s) 261, 329
Csp6I GTAC 1 cut(s) 44
CviAII CATG 1 cut(s) 116
CviJI RGCY 6 cut(s) 65, 89, 214, 239, 270, 317
CviKI_1 RGCY 6 cut(s) 65, 89, 214, 239, 270, 317
CviQI GTAC 1 cut(s) 44
DdeI CTNAG 1 cut(s) 68
DpnI GATC 1 cut(s) 309
DpnII GATC 1 cut(s) 307
EaeI YGGCCR 1 cut(s) 87
EciI GGCGGA 1 cut(s) 239
EcoRII CCWGG 1 cut(s) 271
FaeI CATG 1 cut(s) 119
FaiI YATR 4 cut(s) 105, 113, 117, 299
FaqI GGGAC 2 cut(s) 46, 360
FatI CATG 1 cut(s) 115
Fnu4HI GCNGC 5 cut(s) 15, 54, 57, 222, 240
FokI GGATG 3 cut(s) 185, 314, 365
Fsp4HI GCNGC 5 cut(s) 15, 54, 57, 222, 240
FspBI CTAG 1 cut(s) 39
GluI GCNGC 5 cut(s) 15, 54, 57, 222, 240
HaeIII GGCC 2 cut(s) 89, 270
HapII CCGG 1 cut(s) 318
HgaI GACGC 2 cut(s) 261, 329
Hin1I GRCGYC 1 cut(s) 340
Hin1II CATG 1 cut(s) 119
HinfI GANTC 1 cut(s) 128
HpaII CCGG 1 cut(s) 318
HphI GGTGA 1 cut(s) 194
Hpy166II GTNNAC 2 cut(s) 44, 206
Hpy188III TCNNGA 1 cut(s) 70
Hpy8I GTNNAC 2 cut(s) 44, 206
Hpy99I CGWCG 1 cut(s) 345
HpyAV CCTTC 3 cut(s) 116, 149, 182
HpyCH4III ACNGT 1 cut(s) 77
HpyCH4V TGCA 5 cut(s) 14, 56, 119, 206, 242
HpyF10VI GCNNNNNNNGC 1 cut(s) 62
HpyF3I CTNAG 1 cut(s) 68
Hsp92I GRCGYC 1 cut(s) 340
Hsp92II CATG 1 cut(s) 119
Kzo9I GATC 1 cut(s) 307
LmnI GCTCC 1 cut(s) 226
LpnPI CCDG 7 cut(s) 55, 60, 228, 258, 285, 331, 335
Lsp1109I GCAGC 4 cut(s) 26, 40, 68, 226
LweI GCATC 2 cut(s) 136, 163
MaeI CTAG 1 cut(s) 39
MalI GATC 1 cut(s) 309
MboI GATC 1 cut(s) 307
MboII GAAGA 1 cut(s) 171
MflI RGATCY 1 cut(s) 307
MhlI GDGCHC 1 cut(s) 208
MlsI TGGCCA 1 cut(s) 89
MluNI TGGCCA 1 cut(s) 89
MlyI GAGTC 1 cut(s) 122
MnlI CCTC 7 cut(s) 163, 187, 211, 242, 283, 359, 362
Mox20I TGGCCA 1 cut(s) 89
MscI TGGCCA 1 cut(s) 89
Msp20I TGGCCA 1 cut(s) 89
MspI CCGG 1 cut(s) 318
MspR9I CCNGG 1 cut(s) 273
MvaI CCWGG 1 cut(s) 273
MwoI GCNNNNNNNGC 1 cut(s) 62
NdeII GATC 1 cut(s) 307
NlaIII CATG 1 cut(s) 119
NspI RCATGY 1 cut(s) 119
PkrI GCNGC 5 cut(s) 16, 55, 58, 223, 241
PleI GAGTC 1 cut(s) 122
PpsI GAGTC 1 cut(s) 122
Psp6I CCWGG 1 cut(s) 271
PspGI CCWGG 1 cut(s) 271
PspPI GGNCC 1 cut(s) 269
PstI CTGCAG 1 cut(s) 58
PstNI CAGNNNCTG 1 cut(s) 53
PsuI RGATCY 1 cut(s) 307
RsaI GTAC 1 cut(s) 45
RsaNI GTAC 1 cut(s) 44
SatI GCNGC 5 cut(s) 15, 54, 57, 222, 240
Sau3AI GATC 1 cut(s) 307
Sau96I GGNCC 1 cut(s) 269
SchI GAGTC 1 cut(s) 122
ScrFI CCNGG 1 cut(s) 273
SduI GDGCHC 1 cut(s) 208
SetI ASST 2 cut(s) 67, 198
SfaNI GCATC 2 cut(s) 136, 163
SfcI CTRYAG 1 cut(s) 54
SmlI CTYRAG 1 cut(s) 209
SmoI CTYRAG 1 cut(s) 209
SpeI ACTAGT 1 cut(s) 38
SsiI CCGC 5 cut(s) 94, 176, 221, 224, 293
SspMI CTAG 1 cut(s) 39
StyD4I CCNGG 1 cut(s) 271
TaaI ACNGT 1 cut(s) 77
TauI GCSGC 1 cut(s) 224
TseI GCWGC 4 cut(s) 14, 53, 56, 239
TspDTI ATGAA 2 cut(s) 201, 375
VneI GTGCAC 1 cut(s) 204
XceI RCATGY 1 cut(s) 119
XspI CTAG 1 cut(s) 39
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.