Rh5DG202000

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0018258
Physical Location & Seq
Forward (+)
22376432 .. 22377785
1354 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG202000.1

Sequence Viewer

Length: 279 bp
ATGGCGATGCGTCACAGCGCTGTTTTGGGTTGGGAAGCTGCAGTTGTGGCGGGGCTTACTGGTGGGAGTGGTGGAGGAGGAGAGGTGGCTGAGCTTGGTGGGGACGACAACGGAGGTGGAGATGGAGTTGCGAACCGGTTTCCCAGTTGGTTGCGCCAGACATCCACCTCGGCACTCCTACCCAGAGCCTCCTCCTTGTCACCGACAGCGGCGGCGATCCCCGGCGCTCCGACTGTTGCAGTGTTGCAGCTGGAATTGCTGAATCTTGAATCCTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

92

Amino Acids

9.09

Weight (kDa)

4.55

Isoelectric Point (pI)

44.21

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 50, 209, 212
AclWI GGATC 1 cut(s) 211
AfeI AGCGCT 1 cut(s) 19
AgeI ACCGGT 1 cut(s) 135
AgsI TTSAA 1 cut(s) 269
AluBI AGCT 3 cut(s) 38, 94, 250
AluI AGCT 3 cut(s) 38, 94, 250
AlwI GGATC 1 cut(s) 211
Aor51HI AGCGCT 1 cut(s) 19
ApeKI GCWGC 2 cut(s) 38, 247
AsiGI ACCGGT 1 cut(s) 135
AspLEI GCGC 3 cut(s) 20, 156, 227
AsuC2I CCSGG 1 cut(s) 222
AsuHPI GGTGA 1 cut(s) 192
BbvI GCAGC 2 cut(s) 25, 259
BccI CCATC 1 cut(s) 116
BcnI CCSGG 1 cut(s) 222
BfmI CTRYAG 1 cut(s) 39
BfoI RGCGCY 2 cut(s) 21, 228
BisI GCNGC 4 cut(s) 39, 210, 213, 248
BlpI GCTNAGC 1 cut(s) 90
BlsI GCNGC 4 cut(s) 40, 211, 214, 249
Bme1390I CCNGG 1 cut(s) 222
BmrFI CCNGG 1 cut(s) 222
BmrI ACTGGG 1 cut(s) 138
BmuI ACTGGG 1 cut(s) 138
Bpu1102I GCTNAGC 1 cut(s) 90
BpuMI CCSGG 1 cut(s) 222
BsaJI CCNNGG 2 cut(s) 168, 220
BsaWI WCCGGW 1 cut(s) 135
BsaXI ACNNNNNCTCC 4 cut(s) 69, 99, 111, 141
Bse118I RCCGGY 1 cut(s) 135
Bse1I ACTGG 2 cut(s) 64, 144
BseDI CCNNGG 2 cut(s) 168, 220
BseGI GGATG 1 cut(s) 161
BseMII CTCAG 1 cut(s) 81
BseNI ACTGG 2 cut(s) 64, 144
BseRI GAGGAG 3 cut(s) 90, 93, 181
BseXI GCAGC 2 cut(s) 25, 259
BshTI ACCGGT 1 cut(s) 135
BsiSI CCGG 2 cut(s) 136, 222
BslFI GGGAC 1 cut(s) 116
BsmFI GGGAC 1 cut(s) 116
Bsp143I GATC 1 cut(s) 216
Bsp1720I GCTNAGC 1 cut(s) 90
BspACI CCGC 3 cut(s) 50, 209, 212
BspCNI CTCAG 1 cut(s) 82
BspMAI CTGCAG 1 cut(s) 43
BspPI GGATC 1 cut(s) 211
BsrFI RCCGGY 1 cut(s) 135
BsrI ACTGG 2 cut(s) 64, 144
BssAI RCCGGY 1 cut(s) 135
BssECI CCNNGG 2 cut(s) 168, 220
BssMI GATC 1 cut(s) 216
Bst4CI ACNGT 1 cut(s) 235
BstDEI CTNAG 1 cut(s) 90
BstF5I GGATG 1 cut(s) 161
BstH2I RGCGCY 2 cut(s) 21, 228
BstHHI GCGC 3 cut(s) 20, 156, 227
BstKTI GATC 1 cut(s) 219
BstMBI GATC 1 cut(s) 216
BstMWI GCNNNNNNNGC 2 cut(s) 47, 256
BstSCI CCNGG 1 cut(s) 220
BstSFI CTRYAG 1 cut(s) 39
BstV1I GCAGC 2 cut(s) 25, 259
BtgZI GCGATG 1 cut(s) 20
BtsCI GGATG 1 cut(s) 161
BtsI GCAGTG 1 cut(s) 246
BtsIMutI CAGTG 1 cut(s) 246
CfoI GCGC 3 cut(s) 20, 156, 227
Cfr10I RCCGGY 1 cut(s) 135
CspAI ACCGGT 1 cut(s) 135
CspCI CAANNNNNGTGG 2 cut(s) 97, 132
CviJI RGCY 6 cut(s) 38, 55, 89, 94, 188, 250
CviKI_1 RGCY 6 cut(s) 38, 55, 89, 94, 188, 250
DdeI CTNAG 1 cut(s) 90
DpnI GATC 1 cut(s) 218
DpnII GATC 1 cut(s) 216
Eco47III AGCGCT 1 cut(s) 19
FaqI GGGAC 1 cut(s) 116
FauI CCCGC 1 cut(s) 43
Fnu4HI GCNGC 4 cut(s) 39, 210, 213, 248
FokI GGATG 1 cut(s) 148
Fsp4HI GCNGC 4 cut(s) 39, 210, 213, 248
GlaI GCGC 3 cut(s) 19, 155, 226
GluI GCNGC 4 cut(s) 39, 210, 213, 248
HaeII RGCGCY 2 cut(s) 21, 228
HapII CCGG 2 cut(s) 136, 222
HhaI GCGC 3 cut(s) 20, 156, 227
Hin6I GCGC 3 cut(s) 18, 154, 225
HinP1I GCGC 3 cut(s) 18, 154, 225
HinfI GANTC 2 cut(s) 262, 269
HpaII CCGG 2 cut(s) 136, 222
HphI GGTGA 1 cut(s) 192
Hpy188I TCNGA 1 cut(s) 231
Hpy188III TCNNGA 1 cut(s) 266
HpyCH4III ACNGT 1 cut(s) 235
HpyCH4V TGCA 3 cut(s) 41, 239, 247
HpyF10VI GCNNNNNNNGC 2 cut(s) 47, 256
HpyF3I CTNAG 1 cut(s) 90
HspAI GCGC 3 cut(s) 18, 154, 225
Kzo9I GATC 1 cut(s) 216
LmnI GCTCC 1 cut(s) 232
LpnPI CCDG 7 cut(s) 45, 149, 157, 170, 196, 235, 236
Lsp1109I GCAGC 2 cut(s) 25, 259
MaeIII GTNAC 2 cut(s) 11, 198
MalI GATC 1 cut(s) 218
MboI GATC 1 cut(s) 216
MluCI AATT 1 cut(s) 254
MmeI TCCRAC 1 cut(s) 254
MnlI CCTC 7 cut(s) 68, 71, 76, 107, 178, 199, 202
MspA1I CMGCKG 2 cut(s) 209, 250
MspI CCGG 2 cut(s) 136, 222
MspR9I CCNGG 1 cut(s) 222
MwoI GCNNNNNNNGC 2 cut(s) 47, 256
NciI CCSGG 1 cut(s) 222
NdeII GATC 1 cut(s) 216
NmeAIII GCCGAG 1 cut(s) 149
NmuCI GTSAC 2 cut(s) 11, 198
PfeI GAWTC 2 cut(s) 262, 269
PinAI ACCGGT 1 cut(s) 135
PkrI GCNGC 4 cut(s) 40, 211, 214, 249
PstI CTGCAG 1 cut(s) 43
PvuII CAGCTG 1 cut(s) 250
SatI GCNGC 4 cut(s) 39, 210, 213, 248
Sau3AI GATC 1 cut(s) 216
ScrFI CCNGG 1 cut(s) 222
SetI ASST 6 cut(s) 40, 87, 96, 118, 170, 252
SfcI CTRYAG 1 cut(s) 39
Sse9I AATT 1 cut(s) 254
SsiI CCGC 3 cut(s) 50, 209, 212
StyD4I CCNGG 1 cut(s) 220
TaaI ACNGT 1 cut(s) 235
TasI AATT 1 cut(s) 254
TauI GCSGC 2 cut(s) 212, 215
TfiI GAWTC 2 cut(s) 262, 269
TscAI CASTG 1 cut(s) 246
TseFI GTSAC 2 cut(s) 11, 198
TseI GCWGC 2 cut(s) 38, 247
Tsp45I GTSAC 2 cut(s) 11, 198
TspGWI ACGGA 1 cut(s) 126
TspRI CASTG 1 cut(s) 246
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.