Rh4DG175000

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0018258
Physical Location & Seq
Forward (+)
34786039 .. 34786326
288 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG175000.1

Sequence Viewer

Length: 288 bp
ATGATGGGTTTGGGAAAGAAAGAGGAAGAGGCGGTGGGTGACGAAGGAGAGGCAGGGGCTGTACTTGAGCTAGAGGCGGCGGAGCAAGTGTGGGTCGCAAGTAAAGGACATGGGCTGGAGGTAGGCCCAGGCAGCCTGCTTGACGAGGTGGTCGCTGATGGAGATCTCGTAGTCGGAGTTGGTAGTGGAGTTGGGCGTCAGGGATTGGATGGCGGCGAAGGAGGAAGAGGGAAGGAGGTCCTTGAGGGAGGTGTAGGACTCGGAGCTCTTGAGGGACAGAAGATCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

95

Amino Acids

9.32

Weight (kDa)

4.22

Isoelectric Point (pI)

7.53

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 149
AciI CCGC 4 cut(s) 32, 77, 80, 213
AcyI GRCGYC 1 cut(s) 196
AfaI GTAC 1 cut(s) 63
AjnI CCWGG 1 cut(s) 127
AluBI AGCT 2 cut(s) 70, 266
AluI AGCT 2 cut(s) 70, 266
Alw21I GWGCWC 1 cut(s) 268
AlwNI CAGNNNCTG 1 cut(s) 59
AoxI GGCC 1 cut(s) 124
ApeKI GCWGC 1 cut(s) 132
AspS9I GGNCC 2 cut(s) 125, 238
AsuHPI GGTGA 1 cut(s) 50
AvaII GGWCC 1 cut(s) 238
BanII GRGCYC 1 cut(s) 268
Bbv12I GWGCWC 1 cut(s) 268
BbvI GCAGC 1 cut(s) 144
BccI CCATC 2 cut(s) 152, 203
BciT130I CCWGG 1 cut(s) 129
BfaI CTAG 2 cut(s) 71, 286
BglII AGATCT 2 cut(s) 163, 282
BisI GCNGC 3 cut(s) 78, 133, 214
BlsI GCNGC 3 cut(s) 79, 134, 215
Bme1390I CCNGG 1 cut(s) 129
Bme18I GGWCC 1 cut(s) 238
BmgT120I GGNCC 2 cut(s) 125, 238
BmrFI CCNGG 1 cut(s) 129
BpmI CTGGAG 1 cut(s) 137
BpuEI CTTGAG 2 cut(s) 86, 263
BsaBI GATNNNNATC 1 cut(s) 162
BsaHI GRCGYC 1 cut(s) 196
BsaJI CCNNGG 1 cut(s) 127
BsaXI ACNNNNNCTCC 4 cut(s) 74, 104, 153, 183
Bse8I GATNNNNATC 1 cut(s) 162
BseBI CCWGG 1 cut(s) 129
BseDI CCNNGG 1 cut(s) 127
BseGI GGATG 1 cut(s) 214
BseJI GATNNNNATC 1 cut(s) 162
BseXI GCAGC 1 cut(s) 144
BshFI GGCC 1 cut(s) 126
BsiHKAI GWGCWC 1 cut(s) 268
BsnI GGCC 1 cut(s) 126
Bsp1286I GDGCHC 1 cut(s) 268
Bsp143I GATC 2 cut(s) 163, 282
BspACI CCGC 4 cut(s) 32, 77, 80, 213
BspANI GGCC 1 cut(s) 126
BssECI CCNNGG 1 cut(s) 127
BssMI GATC 2 cut(s) 163, 282
BssNI GRCGYC 1 cut(s) 196
Bst2UI CCWGG 1 cut(s) 129
Bst6I CTCTTC 2 cut(s) 21, 220
BstACI GRCGYC 1 cut(s) 196
BstC8I GCNNGC 1 cut(s) 137
BstF5I GGATG 1 cut(s) 214
BstKTI GATC 2 cut(s) 166, 285
BstMBI GATC 2 cut(s) 163, 282
BstMWI GCNNNNNNNGC 1 cut(s) 132
BstNI CCWGG 1 cut(s) 129
BstSCI CCNGG 1 cut(s) 127
BstV1I GCAGC 1 cut(s) 144
BstX2I RGATCY 2 cut(s) 163, 282
BstYI RGATCY 2 cut(s) 163, 282
BsuRI GGCC 1 cut(s) 126
BtsCI GGATG 1 cut(s) 214
Cac8I GCNNGC 1 cut(s) 137
CaiI CAGNNNCTG 1 cut(s) 59
Cfr13I GGNCC 2 cut(s) 125, 238
CseI GACGC 1 cut(s) 185
Csp6I GTAC 1 cut(s) 62
CviAII CATG 1 cut(s) 110
CviJI RGCY 6 cut(s) 59, 70, 115, 126, 135, 266
CviKI_1 RGCY 6 cut(s) 59, 70, 115, 126, 135, 266
CviQI GTAC 1 cut(s) 62
DpnI GATC 2 cut(s) 165, 284
DpnII GATC 2 cut(s) 163, 282
DrdI GACNNNNNNGTC 1 cut(s) 149
DseDI GACNNNNNNGTC 1 cut(s) 149
Eam1104I CTCTTC 2 cut(s) 21, 220
EarI CTCTTC 2 cut(s) 21, 220
EciI GGCGGA 1 cut(s) 95
Ecl136II GAGCTC 1 cut(s) 266
Eco24I GRGCYC 1 cut(s) 268
Eco47I GGWCC 1 cut(s) 238
Eco53kI GAGCTC 1 cut(s) 266
EcoICRI GAGCTC 1 cut(s) 266
EcoO109I RGGNCCY 1 cut(s) 238
EcoRII CCWGG 1 cut(s) 127
EcoT38I GRGCYC 1 cut(s) 268
FaeI CATG 1 cut(s) 113
FaiI YATR 1 cut(s) 111
FatI CATG 1 cut(s) 109
Fnu4HI GCNGC 3 cut(s) 78, 133, 214
FokI GGATG 1 cut(s) 221
FriOI GRGCYC 1 cut(s) 268
Fsp4HI GCNGC 3 cut(s) 78, 133, 214
FspBI CTAG 2 cut(s) 71, 286
GluI GCNGC 3 cut(s) 78, 133, 214
GsuI CTGGAG 1 cut(s) 137
HaeIII GGCC 1 cut(s) 126
HgaI GACGC 1 cut(s) 185
Hin1I GRCGYC 1 cut(s) 196
Hin1II CATG 1 cut(s) 113
HinfI GANTC 1 cut(s) 258
HphI GGTGA 1 cut(s) 50
Hpy188I TCNGA 2 cut(s) 176, 263
Hpy188III TCNNGA 1 cut(s) 269
HpyAV CCTTC 3 cut(s) 38, 212, 226
HpyF10VI GCNNNNNNNGC 1 cut(s) 132
Hsp92I GRCGYC 1 cut(s) 196
Hsp92II CATG 1 cut(s) 113
Kzo9I GATC 2 cut(s) 163, 282
LmnI GCTCC 2 cut(s) 82, 263
LpnPI CCDG 6 cut(s) 39, 101, 114, 141, 149, 185
Lsp1109I GCAGC 1 cut(s) 144
MaeI CTAG 2 cut(s) 71, 286
MaeIII GTNAC 1 cut(s) 38
MalI GATC 2 cut(s) 165, 284
MboI GATC 2 cut(s) 163, 282
MboII GAAGA 2 cut(s) 38, 237
MflI RGATCY 2 cut(s) 163, 282
MhlI GDGCHC 1 cut(s) 268
MlyI GAGTC 1 cut(s) 252
MmeI TCCRAC 1 cut(s) 154
MspR9I CCNGG 1 cut(s) 129
MvaI CCWGG 1 cut(s) 129
MwoI GCNNNNNNNGC 1 cut(s) 132
NdeII GATC 2 cut(s) 163, 282
NlaIII CATG 1 cut(s) 113
NmuCI GTSAC 1 cut(s) 38
PkrI GCNGC 3 cut(s) 79, 134, 215
PleI GAGTC 1 cut(s) 252
PpsI GAGTC 1 cut(s) 252
PpuMI RGGWCCY 1 cut(s) 238
Psp124BI GAGCTC 1 cut(s) 268
Psp5II RGGWCCY 1 cut(s) 238
Psp6I CCWGG 1 cut(s) 127
PspGI CCWGG 1 cut(s) 127
PspPI GGNCC 2 cut(s) 125, 238
PspPPI RGGWCCY 1 cut(s) 238
PstNI CAGNNNCTG 1 cut(s) 59
PsuI RGATCY 2 cut(s) 163, 282
RsaI GTAC 1 cut(s) 63
RsaNI GTAC 1 cut(s) 62
SacI GAGCTC 1 cut(s) 268
SatI GCNGC 3 cut(s) 78, 133, 214
Sau3AI GATC 2 cut(s) 163, 282
Sau96I GGNCC 2 cut(s) 125, 238
SchI GAGTC 1 cut(s) 252
ScrFI CCNGG 1 cut(s) 129
SduI GDGCHC 1 cut(s) 268
SetI ASST 6 cut(s) 72, 123, 150, 240, 253, 268
SinI GGWCC 1 cut(s) 238
SmlI CTYRAG 3 cut(s) 65, 242, 269
SmoI CTYRAG 3 cut(s) 65, 242, 269
SsiI CCGC 4 cut(s) 32, 77, 80, 213
SspMI CTAG 2 cut(s) 71, 286
SstI GAGCTC 1 cut(s) 268
StyD4I CCNGG 1 cut(s) 127
TatI WGTACW 1 cut(s) 61
TauI GCSGC 2 cut(s) 80, 216
TseFI GTSAC 1 cut(s) 38
TseI GCWGC 1 cut(s) 132
Tsp45I GTSAC 1 cut(s) 38
VpaK11BI GGWCC 1 cut(s) 238
XspI CTAG 2 cut(s) 71, 286
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.