Rmu_sc0023319.1_g000003

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0023319.1
OG0016446
Physical Location & Seq
Forward (+)
19281 .. 20366
1086 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0023319.1_g000003.1.cds

Sequence Viewer

Length: 1086 bp
atgagaaaagtgtggaagtacctcgaggagcattttgacacgaacttgaatgagttacagaccagactccaagagcttcagatccgcgaggaggaaattagtaaccgagaaaagcgattggaagacagagagtccgagttggaatgtcttgataagctgattgaatataagggagaggaatatgatgcaatggagcaggcaataacagagaaagagttacaggttgaacaagtgaagaccgagttgcaatccattagtttattggttgaagaaaaaagccaagagcttgatagtaaagagaagcgggttttggaggttgaggagttgcttaggaaaaaggataaggagtgtgctttgatgcaaaaccgtatcgaagagggaaggaataatttgattagggttgagaaaagtattagggaaatggatatcaaagtgaagacttctatggaggattggtccggtaaggttgatttaaaagagaggcaaattgaaggactgctcaagccaaatgtggaacaacttcttcttgcttataacatgatcaaagaatacttcaaagaagttgaaaggaaacagaggggactgaaacaacaggccaaagaccttgaatcaaaagagagagaagttgctttgaaagttgatgaacttaagctgactgaaacaagggttaaaaaacgcctcaataatgttcagttgaaagaaaagcattttgattcactggaaaagtcattacaagcagacaaacagcatttgggttcactcctaaagtccacagaagagtgcaaaaaggatttggcgtcagtgtcccatcaacttcagatgaaagaaagggagcttgcacagcaggccaaagagcttgaattgaagcagaaacaattttcttctcgagcgaaaactaagcatttggaacctgcccctgctgacaacaatgctactgttccttcatctacaattgatcattcctgcatcaatatagatggtacaacctcgcaatccttgattaatgcaagtcataagaagtttggtttttttcctacctatgagaatgttgttcacagctatcagcatttcttgatgtttttctag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

361

Amino Acids

42.58

Weight (kDa)

5.89

Isoelectric Point (pI)

50.69

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 534
AbsI CCTCGAGG 1 cut(s) 23
Acc36I ACCTGC 1 cut(s) 919
AccII CGCG 1 cut(s) 87
AciI CCGC 2 cut(s) 85, 304
AclWI GGATC 1 cut(s) 76
AcuI CTGAAG 2 cut(s) 62, 800
AcyI GRCGYC 1 cut(s) 797
AfaI GTAC 2 cut(s) 20, 982
AfiI CCNNNNNNNGG 1 cut(s) 91
AflII CTTAAG 1 cut(s) 647
AhdI GACNNNNNGTC 1 cut(s) 130
AjuI GAANNNNNNNTTGG 2 cut(s) 293, 325
AluBI AGCT 7 cut(s) 76, 157, 286, 652, 835, 856, 1059
AluI AGCT 7 cut(s) 76, 157, 286, 652, 835, 856, 1059
AlwI GGATC 1 cut(s) 76
Ama87I CYCGRG 2 cut(s) 23, 885
AoxI GGCC 2 cut(s) 594, 846
AseI ATTAAT 1 cut(s) 1002
Asp700I GAANNNNTTC 1 cut(s) 519
AspS9I GGNCC 1 cut(s) 456
AvaI CYCGRG 2 cut(s) 23, 885
AvaII GGWCC 1 cut(s) 456
BbsI GAAGAC 3 cut(s) 129, 242, 443
BccI CCATC 2 cut(s) 816, 971
BclI TGATCA 2 cut(s) 540, 955
BfaI CTAG 1 cut(s) 1084
BfrI CTTAAG 1 cut(s) 647
BfuAI ACCTGC 1 cut(s) 919
Bme18I GGWCC 1 cut(s) 456
BmeRI GACNNNNNGTC 1 cut(s) 130
BmeT110I CYCGRG 2 cut(s) 23, 885
BmgT120I GGNCC 1 cut(s) 456
BmiI GGNNCC 1 cut(s) 909
BmsI GCATC 3 cut(s) 175, 348, 975
BpiI GAAGAC 3 cut(s) 129, 242, 443
Bpu10I CCTNAGC 1 cut(s) 329
BpuEI CTTGAG 1 cut(s) 485
BsaHI GRCGYC 1 cut(s) 797
BsaWI WCCGGW 1 cut(s) 458
BsaXI ACNNNNNCTCC 2 cut(s) 440, 470
Bsc4I CCNNNNNNNGG 1 cut(s) 91
Bse1I ACTGG 1 cut(s) 723
Bse3DI GCAATG 1 cut(s) 195
BseLI CCNNNNNNNGG 1 cut(s) 91
BseMI GCAATG 1 cut(s) 195
BseNI ACTGG 1 cut(s) 723
BseRI GAGGAG 3 cut(s) 41, 104, 335
Bsh1236I CGCG 1 cut(s) 87
BshFI GGCC 2 cut(s) 596, 848
BsiHKCI CYCGRG 2 cut(s) 23, 885
BsiSI CCGG 1 cut(s) 459
BslFI GGGAC 2 cut(s) 594, 790
BslI CCNNNNNNNGG 1 cut(s) 91
BsmFI GGGAC 2 cut(s) 594, 790
BsnI GGCC 2 cut(s) 596, 848
BsoBI CYCGRG 2 cut(s) 23, 885
Bsp143I GATC 3 cut(s) 81, 540, 955
BspACI CCGC 2 cut(s) 85, 304
BspANI GGCC 2 cut(s) 596, 848
BspFNI CGCG 1 cut(s) 87
BspLI GGNNCC 1 cut(s) 909
BspMI ACCTGC 1 cut(s) 919
BspPI GGATC 1 cut(s) 76
BspTI CTTAAG 1 cut(s) 647
BsrDI GCAATG 1 cut(s) 195
BsrI ACTGG 1 cut(s) 723
BssMI GATC 3 cut(s) 81, 540, 955
BssNI GRCGYC 1 cut(s) 797
Bst4CI ACNGT 2 cut(s) 368, 937
Bst6I CTCTTC 2 cut(s) 369, 771
BstACI GRCGYC 1 cut(s) 797
BstAFI CTTAAG 1 cut(s) 647
BstC8I GCNNGC 3 cut(s) 198, 837, 846
BstDEI CTNAG 2 cut(s) 329, 897
BstFNI CGCG 1 cut(s) 87
BstKTI GATC 3 cut(s) 84, 543, 958
BstMBI GATC 3 cut(s) 81, 540, 955
BstMWI GCNNNNNNNGC 2 cut(s) 841, 845
BstUI CGCG 1 cut(s) 87
BstV2I GAAGAC 3 cut(s) 129, 242, 443
BstX2I RGATCY 1 cut(s) 81
BstYI RGATCY 1 cut(s) 81
BsuRI GGCC 2 cut(s) 596, 848
BtsIMutI CAGTG 2 cut(s) 716, 807
BveI ACCTGC 1 cut(s) 919
Cac8I GCNNGC 3 cut(s) 198, 837, 846
Cfr13I GGNCC 1 cut(s) 456
CseI GACGC 1 cut(s) 786
Csp6I GTAC 2 cut(s) 19, 981
CviAII CATG 1 cut(s) 538
CviQI GTAC 2 cut(s) 19, 981
DdeI CTNAG 2 cut(s) 329, 897
DpnI GATC 3 cut(s) 83, 542, 957
DpnII GATC 3 cut(s) 81, 540, 955
DraI TTTAAA 1 cut(s) 474
DriI GACNNNNNGTC 1 cut(s) 130
Eam1104I CTCTTC 2 cut(s) 369, 771
Eam1105I GACNNNNNGTC 1 cut(s) 130
EarI CTCTTC 2 cut(s) 369, 771
Eco32I GATATC 1 cut(s) 427
Eco47I GGWCC 1 cut(s) 456
Eco57I CTGAAG 2 cut(s) 62, 800
Eco88I CYCGRG 2 cut(s) 23, 885
EcoRV GATATC 1 cut(s) 427
FaeI CATG 1 cut(s) 541
FaiI YATR 8 cut(s) 168, 183, 446, 534, 539, 974, 1014, 1041
FaqI GGGAC 2 cut(s) 594, 790
FatI CATG 1 cut(s) 537
FauI CCCGC 1 cut(s) 297
FbaI TGATCA 2 cut(s) 540, 955
FspBI CTAG 1 cut(s) 1084
HaeIII GGCC 2 cut(s) 596, 848
HapII CCGG 1 cut(s) 459
HgaI GACGC 1 cut(s) 786
Hin1I GRCGYC 1 cut(s) 797
Hin1II CATG 1 cut(s) 541
HinfI GANTC 4 cut(s) 66, 131, 608, 713
HpaII CCGG 1 cut(s) 459
Hpy166II GTNNAC 3 cut(s) 758, 771, 1054
Hpy188I TCNGA 3 cut(s) 81, 136, 819
Hpy188III TCNNGA 3 cut(s) 149, 885, 1072
Hpy8I GTNNAC 3 cut(s) 758, 771, 1054
HpyAV CCTTC 3 cut(s) 375, 485, 951
HpyCH4III ACNGT 2 cut(s) 368, 937
HpyCH4V TGCA 7 cut(s) 188, 247, 361, 783, 839, 966, 1007
HpyF10VI GCNNNNNNNGC 2 cut(s) 841, 845
HpyF3I CTNAG 2 cut(s) 329, 897
Hsp92I GRCGYC 1 cut(s) 797
Hsp92II CATG 1 cut(s) 541
Ksp22I TGATCA 2 cut(s) 540, 955
Kzo9I GATC 3 cut(s) 81, 540, 955
LmnI GCTCC 3 cut(s) 28, 193, 832
LweI GCATC 3 cut(s) 175, 348, 975
MaeI CTAG 1 cut(s) 1084
MaeIII GTNAC 3 cut(s) 54, 101, 216
MalI GATC 3 cut(s) 83, 542, 957
MboI GATC 3 cut(s) 81, 540, 955
MboII GAAGA 8 cut(s) 134, 247, 281, 386, 448, 515, 788, 873
MfeI CAATTG 1 cut(s) 951
MflI RGATCY 1 cut(s) 81
MluCI AATT 6 cut(s) 96, 388, 486, 860, 875, 951
MlyI GAGTC 2 cut(s) 60, 140
MmeI TCCRAC 1 cut(s) 120
MroXI GAANNNNTTC 1 cut(s) 519
MseI TTAA 4 cut(s) 473, 648, 669, 1002
MspCI CTTAAG 1 cut(s) 647
MspI CCGG 1 cut(s) 459
MunI CAATTG 1 cut(s) 951
MvnI CGCG 1 cut(s) 87
MwoI GCNNNNNNNGC 2 cut(s) 841, 845
NdeII GATC 3 cut(s) 81, 540, 955
NlaIII CATG 1 cut(s) 541
NlaIV GGNNCC 1 cut(s) 909
PaeR7I CTCGAG 2 cut(s) 23, 885
PdmI GAANNNNTTC 1 cut(s) 519
PfeI GAWTC 2 cut(s) 608, 713
PleI GAGTC 2 cut(s) 60, 139
PpsI GAGTC 2 cut(s) 60, 139
PshBI ATTAAT 1 cut(s) 1002
PsiI TTATAA 1 cut(s) 534
PspN4I GGNNCC 1 cut(s) 909
PspPI GGNCC 1 cut(s) 456
PspXI VCTCGAGB 1 cut(s) 23
PsuI RGATCY 1 cut(s) 81
RsaI GTAC 2 cut(s) 20, 982
RsaNI GTAC 2 cut(s) 19, 981
SaqAI TTAA 4 cut(s) 473, 648, 669, 1002
Sau3AI GATC 3 cut(s) 81, 540, 955
Sau96I GGNCC 1 cut(s) 456
SchI GAGTC 2 cut(s) 60, 140
SfaNI GCATC 3 cut(s) 175, 348, 975
Sfr274I CTCGAG 2 cut(s) 23, 885
SinI GGWCC 1 cut(s) 456
SlaI CTCGAG 2 cut(s) 23, 885
SmlI CTYRAG 4 cut(s) 23, 500, 647, 885
SmoI CTYRAG 4 cut(s) 23, 500, 647, 885
Sse9I AATT 6 cut(s) 96, 388, 486, 860, 875, 951
SsiI CCGC 2 cut(s) 85, 304
SspMI CTAG 1 cut(s) 1084
TaaI ACNGT 2 cut(s) 368, 937
TaqI TCGA 3 cut(s) 24, 372, 886
TaqII GACCGA 1 cut(s) 254
TasI AATT 6 cut(s) 96, 388, 486, 860, 875, 951
TfiI GAWTC 2 cut(s) 608, 713
Tru1I TTAA 4 cut(s) 473, 648, 669, 1002
Tru9I TTAA 4 cut(s) 473, 648, 669, 1002
TscAI CASTG 2 cut(s) 723, 807
TspDTI ATGAA 3 cut(s) 657, 836, 933
TspRI CASTG 2 cut(s) 723, 807
Vha464I CTTAAG 1 cut(s) 647
VpaK11BI GGWCC 1 cut(s) 456
VspI ATTAAT 1 cut(s) 1002
XcmI CCANNNNNNNNNTGG 1 cut(s) 259
XhoI CTCGAG 2 cut(s) 23, 885
XmnI GAANNNNTTC 1 cut(s) 519
XspI CTAG 1 cut(s) 1084
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.