Rw3G003730

Frigida-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0016446
Physical Location & Seq
Forward (+)
3197934 .. 3200638
2705 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G003730.1

Sequence Viewer

Length: 1047 bp
ATGCAAAACCGTATCGAAGAGGGAAGGAATAATTTGATTAGGGTTGAGAAAAGTATTAGGGAAATGGATATCAAAGTGAAGACTTCTATGGAGTATTGGTCCGGTAAGGTTGATTTAAAAGAGAGGCAAATTGAAGGACTGCTCATGCCAAATGTGGAACAACTTCTTCTTGCTTATAACATGATCAAAGAATACTTCAAAGAAGTTGAAAGGAAAGAGAGGGGACTGAAACAACAGGCCAAAGACCTTGAATCAAAAGAGAGAGAAGTTGCTTTGAAAGTTGATGAACTTAAGCTGACTGAAACAAGGGTTAAAAAACGCCTCAATAATGTTCAGTTGAAAGAAAAGCATTTTGATTCACTGGAAAAGTCATTAAAAGCAGACAAACAGCATTTGGGTTCACTCCTAAAGTCCACAGAAGAGTGCAAAAAGGATTTGGCTTCAATATCCCATCAACTTCAGATGAAAGAAAGGGAGCTTGCACAGCAGGCCAAAGAGCTTGAATTGAAGCAGAAACAATTTTCTTCTCAAGCGAAAACTAAGCATTTGGAACCTGCCCCTGCTGATAACAATGCCACTGTTCCTTCATCTACAATTGATCATTCCTGCATCAATATAGATGGTATTATTTGGAATCTGATAGAAAGGGGTCGACTGATTGAGGCTGTTAGATTTATTTGCAGCTTCAAGTTAACTGAAAAGCTCCCCCCAGTACCACTCTTGAAAGAGTATGTGGAAGATGCAAAGAAGTTCTGTGAAATATTTTGTGAAAAAAAGATATCTCTTGATTTAAAGGCTGAGTTTGTAGACAACAAAATAGGTGGTGTAAAAGCTGTGCAGCAATGCATCAAAGATTACAATCTTGAGTGCGAATACCCAGCTGCTGACATTCAAATGCAAGTAGTTCAGTTCGAAAGAATGAAGGAGAAATGGAGAAGTCCGGCACCAAATCTTGGCTCCAATGTCAAACAAAAAGGGCAGAGAAAAAGAAAGAGACCTAATTCTAGCAGTTCTACCCCTGAGTCTCAACCCCAGCAACAAAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Frigida

Protein Analysis

348

Amino Acids

40.4

Weight (kDa)

9.17

Isoelectric Point (pI)

44.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Frigida PF07899 206 - 311 1.3e-23 Frigida-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 177
Acc36I ACCTGC 1 cut(s) 562
AccB1I GGYRCC 1 cut(s) 943
AccB7I CCANNNNNTGG 1 cut(s) 953
AccI GTMKAC 2 cut(s) 652, 807
AcuI CTGAAG 1 cut(s) 443
AfaI GTAC 1 cut(s) 714
AfiI CCNNNNNNNGG 1 cut(s) 953
AflII CTTAAG 1 cut(s) 290
AluBI AGCT 7 cut(s) 295, 478, 499, 684, 703, 833, 881
AluI AGCT 7 cut(s) 295, 478, 499, 684, 703, 833, 881
Alw26I GTCTC 2 cut(s) 988, 1029
AlwNI CAGNNNCTG 1 cut(s) 884
AoxI GGCC 2 cut(s) 237, 489
ApeKI GCWGC 3 cut(s) 681, 838, 881
Asp700I GAANNNNTTC 1 cut(s) 162
AspS9I GGNCC 1 cut(s) 99
AsuII TTCGAA 1 cut(s) 912
AvaII GGWCC 1 cut(s) 99
BanI GGYRCC 1 cut(s) 943
BbsI GAAGAC 1 cut(s) 86
BbvI GCAGC 3 cut(s) 693, 850, 868
BccI CCATC 2 cut(s) 459, 614
BclI TGATCA 2 cut(s) 183, 598
BcoDI GTCTC 2 cut(s) 988, 1029
BfaI CTAG 1 cut(s) 1005
BfrI CTTAAG 1 cut(s) 290
BfuAI ACCTGC 1 cut(s) 562
BisI GCNGC 3 cut(s) 682, 839, 882
BlsI GCNGC 3 cut(s) 683, 840, 883
Bme18I GGWCC 1 cut(s) 99
BmgT120I GGNCC 1 cut(s) 99
BmiI GGNNCC 3 cut(s) 552, 945, 958
BmrI ACTGGG 1 cut(s) 704
BmsI GCATC 3 cut(s) 618, 730, 855
BmuI ACTGGG 1 cut(s) 704
BpiI GAAGAC 1 cut(s) 86
Bpu14I TTCGAA 1 cut(s) 912
BpuEI CTTGAG 2 cut(s) 513, 884
BsaBI GATNNNNATC 1 cut(s) 858
BsaI GGTCTC 1 cut(s) 988
BsaWI WCCGGW 1 cut(s) 101
BsaXI ACNNNNNCTCC 2 cut(s) 83, 113
Bsc4I CCNNNNNNNGG 1 cut(s) 953
Bse1I ACTGG 2 cut(s) 366, 710
Bse3DI GCAATG 1 cut(s) 848
Bse8I GATNNNNATC 1 cut(s) 858
BseJI GATNNNNATC 1 cut(s) 858
BseLI CCNNNNNNNGG 1 cut(s) 953
BseMI GCAATG 1 cut(s) 848
BseMII CTCAG 2 cut(s) 789, 1011
BseNI ACTGG 2 cut(s) 366, 710
BseXI GCAGC 3 cut(s) 693, 850, 868
BseYI CCCAGC 2 cut(s) 877, 1032
BsgI GTGCAG 1 cut(s) 857
BshFI GGCC 2 cut(s) 239, 491
BshNI GGYRCC 1 cut(s) 943
BsiSI CCGG 2 cut(s) 102, 941
BslFI GGGAC 1 cut(s) 237
BslI CCNNNNNNNGG 1 cut(s) 953
BsmAI GTCTC 2 cut(s) 988, 1029
BsmFI GGGAC 1 cut(s) 237
BsnI GGCC 2 cut(s) 239, 491
Bso31I GGTCTC 1 cut(s) 988
Bsp119I TTCGAA 1 cut(s) 912
Bsp143I GATC 2 cut(s) 183, 598
BspANI GGCC 2 cut(s) 239, 491
BspCNI CTCAG 2 cut(s) 790, 1012
BspLI GGNNCC 3 cut(s) 552, 945, 958
BspMI ACCTGC 1 cut(s) 562
BspT104I TTCGAA 1 cut(s) 912
BspT107I GGYRCC 1 cut(s) 943
BspTI CTTAAG 1 cut(s) 290
BspTNI GGTCTC 1 cut(s) 988
BsrDI GCAATG 1 cut(s) 848
BsrI ACTGG 2 cut(s) 366, 710
BssMI GATC 2 cut(s) 183, 598
Bst4CI ACNGT 2 cut(s) 11, 580
Bst6I CTCTTC 2 cut(s) 12, 414
BstAFI CTTAAG 1 cut(s) 290
BstBI TTCGAA 1 cut(s) 912
BstC8I GCNNGC 2 cut(s) 480, 489
BstDEI CTNAG 3 cut(s) 540, 798, 1020
BstKTI GATC 2 cut(s) 186, 601
BstMAI GTCTC 2 cut(s) 988, 1029
BstMBI GATC 2 cut(s) 183, 598
BstMWI GCNNNNNNNGC 2 cut(s) 484, 488
BstV1I GCAGC 3 cut(s) 693, 850, 868
BstV2I GAAGAC 1 cut(s) 86
BsuRI GGCC 2 cut(s) 239, 491
BtsIMutI CAGTG 2 cut(s) 359, 576
BveI ACCTGC 1 cut(s) 562
Cac8I GCNNGC 2 cut(s) 480, 489
CaiI CAGNNNCTG 1 cut(s) 884
Cfr13I GGNCC 1 cut(s) 99
Csp6I GTAC 1 cut(s) 713
CspCI CAANNNNNGTGG 2 cut(s) 802, 837
CviAII CATG 2 cut(s) 145, 181
CviQI GTAC 1 cut(s) 713
DdeI CTNAG 3 cut(s) 540, 798, 1020
DpnI GATC 2 cut(s) 185, 600
DpnII GATC 2 cut(s) 183, 598
DraI TTTAAA 2 cut(s) 117, 792
Eam1104I CTCTTC 2 cut(s) 12, 414
EarI CTCTTC 2 cut(s) 12, 414
Eco31I GGTCTC 1 cut(s) 988
Eco32I GATATC 2 cut(s) 70, 780
Eco47I GGWCC 1 cut(s) 99
Eco57I CTGAAG 1 cut(s) 443
EcoRV GATATC 2 cut(s) 70, 780
EcoT22I ATGCAT 1 cut(s) 848
FaeI CATG 2 cut(s) 148, 184
FaiI YATR 6 cut(s) 89, 146, 177, 182, 617, 732
FaqI GGGAC 1 cut(s) 237
FatI CATG 2 cut(s) 144, 180
FbaI TGATCA 2 cut(s) 183, 598
FblI GTMKAC 2 cut(s) 652, 807
Fnu4HI GCNGC 3 cut(s) 682, 839, 882
Fsp4HI GCNGC 3 cut(s) 682, 839, 882
FspBI CTAG 1 cut(s) 1005
GluI GCNGC 3 cut(s) 682, 839, 882
GsaI CCCAGC 2 cut(s) 881, 1036
HaeIII GGCC 2 cut(s) 239, 491
HapII CCGG 2 cut(s) 102, 941
Hin1II CATG 2 cut(s) 148, 184
HincII GTYRAC 2 cut(s) 653, 693
HindII GTYRAC 2 cut(s) 653, 693
HinfI GANTC 4 cut(s) 251, 356, 634, 1022
HpaI GTTAAC 1 cut(s) 693
HpaII CCGG 2 cut(s) 102, 941
Hpy166II GTNNAC 5 cut(s) 401, 414, 653, 693, 808
Hpy188I TCNGA 2 cut(s) 462, 639
Hpy188III TCNNGA 3 cut(s) 721, 785, 863
Hpy8I GTNNAC 5 cut(s) 401, 414, 653, 693, 808
HpyAV CCTTC 4 cut(s) 18, 128, 594, 916
HpyCH4III ACNGT 2 cut(s) 11, 580
HpyCH4V TGCA 9 cut(s) 4, 426, 482, 609, 681, 743, 838, 846, 898
HpyF10VI GCNNNNNNNGC 2 cut(s) 484, 488
HpyF3I CTNAG 3 cut(s) 540, 798, 1020
Hsp92II CATG 2 cut(s) 148, 184
Ksp22I TGATCA 2 cut(s) 183, 598
KspAI GTTAAC 1 cut(s) 693
Kzo9I GATC 2 cut(s) 183, 598
LmnI GCTCC 3 cut(s) 475, 708, 962
Lsp1109I GCAGC 3 cut(s) 693, 850, 868
LweI GCATC 3 cut(s) 618, 730, 855
MaeI CTAG 1 cut(s) 1005
MalI GATC 2 cut(s) 185, 600
MboI GATC 2 cut(s) 183, 598
MboII GAAGA 6 cut(s) 29, 91, 158, 431, 516, 749
MfeI CAATTG 1 cut(s) 594
MluCI AATT 6 cut(s) 31, 129, 503, 518, 594, 1000
MlyI GAGTC 1 cut(s) 1031
MnlI CCTC 5 cut(s) 13, 117, 213, 332, 655
Mph1103I ATGCAT 1 cut(s) 848
MroXI GAANNNNTTC 1 cut(s) 162
MseI TTAA 6 cut(s) 116, 291, 312, 374, 692, 791
MslI CAYNNNNRTG 1 cut(s) 893
MspA1I CMGCKG 1 cut(s) 881
MspCI CTTAAG 1 cut(s) 290
MspI CCGG 2 cut(s) 102, 941
MunI CAATTG 1 cut(s) 594
MwoI GCNNNNNNNGC 2 cut(s) 484, 488
NdeII GATC 2 cut(s) 183, 598
NlaIII CATG 2 cut(s) 148, 184
NlaIV GGNNCC 3 cut(s) 552, 945, 958
NsiI ATGCAT 1 cut(s) 848
NspV TTCGAA 1 cut(s) 912
PdmI GAANNNNTTC 1 cut(s) 162
PfeI GAWTC 3 cut(s) 251, 356, 634
PflMI CCANNNNNTGG 1 cut(s) 953
PkrI GCNGC 3 cut(s) 683, 840, 883
PleI GAGTC 1 cut(s) 1030
PpsI GAGTC 1 cut(s) 1030
PsiI TTATAA 1 cut(s) 177
PspFI CCCAGC 2 cut(s) 877, 1032
PspN4I GGNNCC 3 cut(s) 552, 945, 958
PspPI GGNCC 1 cut(s) 99
PstNI CAGNNNCTG 1 cut(s) 884
PvuII CAGCTG 1 cut(s) 881
RsaI GTAC 1 cut(s) 714
RsaNI GTAC 1 cut(s) 713
RseI CAYNNNNRTG 1 cut(s) 893
SalI GTCGAC 1 cut(s) 651
SaqAI TTAA 6 cut(s) 116, 291, 312, 374, 692, 791
SatI GCNGC 3 cut(s) 682, 839, 882
Sau3AI GATC 2 cut(s) 183, 598
Sau96I GGNCC 1 cut(s) 99
SchI GAGTC 1 cut(s) 1031
SfaNI GCATC 3 cut(s) 618, 730, 855
SfuI TTCGAA 1 cut(s) 912
SinI GGWCC 1 cut(s) 99
SmiMI CAYNNNNRTG 1 cut(s) 893
SmlI CTYRAG 3 cut(s) 290, 528, 863
SmoI CTYRAG 3 cut(s) 290, 528, 863
Sse9I AATT 6 cut(s) 31, 129, 503, 518, 594, 1000
SspI AATATT 1 cut(s) 762
SspMI CTAG 1 cut(s) 1005
TaaI ACNGT 2 cut(s) 11, 580
TaqI TCGA 3 cut(s) 15, 652, 912
TasI AATT 6 cut(s) 31, 129, 503, 518, 594, 1000
TfiI GAWTC 3 cut(s) 251, 356, 634
Tru1I TTAA 6 cut(s) 116, 291, 312, 374, 692, 791
Tru9I TTAA 6 cut(s) 116, 291, 312, 374, 692, 791
TscAI CASTG 2 cut(s) 366, 583
TseI GCWGC 3 cut(s) 681, 838, 881
TspDTI ATGAA 4 cut(s) 300, 479, 576, 935
TspRI CASTG 2 cut(s) 366, 583
Van91I CCANNNNNTGG 1 cut(s) 953
Vha464I CTTAAG 1 cut(s) 290
VpaK11BI GGWCC 1 cut(s) 99
XmiI GTMKAC 2 cut(s) 652, 807
XmnI GAANNNNTTC 1 cut(s) 162
XspI CTAG 1 cut(s) 1005
Zsp2I ATGCAT 1 cut(s) 848
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.