Rroxscaffold_6G00430110

FRIGIDA-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0016446
Physical Location & Seq
Forward (+)
50311061 .. 50318062
7002 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00430110.1

Sequence Viewer

Length: 1692 bp
ATGGACTCTATGAGAAAAGCGTGGAAGTACCTCGAGGAGCAGTTTGACACAAACTTGAATGAGTTACAGACCAGACTCCAAGAGCTTCAGATCCGCGAGGAGGAAATTAGTAACAAAGAAAAGCGATTGGAAGACAGAGAGTCCGAGTTGGAACGTCTTGATAAGCTGATTGAATATAAGGGAGAGGAATATGATGCAATGGAGGAGGCAATAACAGAGAAGCAGTTACAGGTTGAACAAGTGAAAACCGAGTTGCAATCCATTAGTTTATTGGTTGAAGAAAAAAGCCAAGAGCTTGATAGTAAAGAGAAGCGGGTTTTGGAGGTTGAGGAGTTGCTTAGGAAAAAGGATAAGGAGTGTGCTTTGATGCAAGACCGTATCGAAGAGGGAAGGAATAATTTGATTAGGGTTGAGAAAAGTATTAGGGAAATGGATATCAAAGTGAAGACTTCTATGGAGGATTGGTCTGGTAAGGTTGATTTAAAAGAGAGGCAAATTGAAGGACTGCTCAAGCCAAATGTGGAACAACTTCTTCTTGCTTATAACATGATCAAAGAATACTTCAATGAAGTTGAAAGGAAAGAGAGGGGACTGAAACAACAGGCCAAAGACCTTGAATCAAAAGACAGAGAAGTTGCTTTGAAAGCTGATGAACTTAAGCTGACTGAAACGAGGGTTAAAAAACGCCTCAATAAGGTTCAGTTGAAAGAAAAGCATTTTGATTCACTGGAAAAGTCATTAAAAGCAGACAAACAGCATTTGGGTTCACTCCTAAAGTCCACAGAAGAGTGCAAAAAGGATTTGGCTTCAATATCCCATCAACTTCAGATGAAAGAAAGGAAGCTTGCACAGCAGGCCAAAGAGCTTGAATTGAAGCAGAAACAATTTTCTTCTCAAGCGAAAACTAAGCATTTGGAACCTGCCCCTGCTGATAAAAATGCCACTTCTCCTTCATCTACAATTGATCATTCCTGCATCAATATAGATGTGACGTCCAACAAAAAGGAACCAGAATCTTCACCGACTAGAAATGCAGCAAATCTTCAACCAAATACCACCAGGGATGACAGGAATGTGCCAGGGTTGCTAAATGAGAATTTTAGTTGTAATCATTCAGTACAGAGTGACTTGTCTGCTTATCTTCGGTCAGTACCAGACCCAGCTAAAGTTGTGTTGAACAGCATGCGAAATTCTATTAGGCCATACTTGAGGAAGGGATATTTTGAAGAAAGTGTAATGTCACGAAACATTTCCTTATTGAAGGAGCTAATGATTGTTTCACCGCATGTTGGATCCCATCTGAAAGAAAATGCAAGATATCTAGCAACCCAGTGGAAAATAAAGATGCGAGGCAATACTGAGAATTCAGTGGAGAGTTTGGCATATTTTCTGTTTATAACTATGTATGAGTTGGATTTTATGTTGAATGGAGATGAGATTGTAAAGCTTCTTCTGGTGATTTCTCAGACAATTACGGCTCTAGAATTATGTCAGACACATGGTTTTGCAGCTAAGATGATAGGTATTATTCGGAATCTGATAGAAAGGGATCGACTGATTGAGGCTGTTAGATTTATTTGCAGCTTCAAGTTAACTGAAAAGATCCCCCCAGTACCACTCTTGAGAGAGTATGTGGAAGATGCAAAGAAGTTCTGTGAAATATTTTGTGAAAAAAAAAATCTCTTGATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04530 map04530
Pfam Domains
Protein Families
Frigida

Protein Analysis

563

Amino Acids

65.55

Weight (kDa)

6.42

Isoelectric Point (pI)

49.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Frigida PF07899 363 - 559 1.2e-37 Frigida-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 543, 1397
AatII GACGTC 1 cut(s) 995
AbsI CCTCGAGG 1 cut(s) 32
Acc36I ACCTGC 1 cut(s) 928
AccII CGCG 1 cut(s) 96
AciI CCGC 3 cut(s) 94, 313, 1283
AclWI GGATC 5 cut(s) 85, 1287, 1300, 1557, 1597
AcsI RAATTY 3 cut(s) 1096, 1189, 1363
AcuI CTGAAG 2 cut(s) 71, 809
AcyI GRCGYC 1 cut(s) 992
AfaI GTAC 4 cut(s) 29, 1119, 1152, 1614
AfiI CCNNNNNNNGG 3 cut(s) 100, 694, 1289
AflII CTTAAG 1 cut(s) 656
AhdI GACNNNNNGTC 1 cut(s) 139
AjnI CCWGG 2 cut(s) 1058, 1078
AjuI GAANNNNNNNTTGG 2 cut(s) 302, 334
AlwI GGATC 5 cut(s) 85, 1287, 1300, 1557, 1597
Ama87I CYCGRG 1 cut(s) 32
AoxI GGCC 3 cut(s) 603, 855, 1199
ApeKI GCWGC 3 cut(s) 1034, 1508, 1581
ApoI RAATTY 3 cut(s) 1096, 1189, 1363
Asp700I GAANNNNTTC 2 cut(s) 528, 1249
AsuHPI GGTGA 3 cut(s) 1011, 1272, 1468
AvaI CYCGRG 1 cut(s) 32
BamHI GGATCC 1 cut(s) 1292
BarI GAAGNNNNNNTAC 2 cut(s) 1434, 1466
BbsI GAAGAC 2 cut(s) 138, 452
BbvI GCAGC 3 cut(s) 1046, 1520, 1593
BccI CCATC 2 cut(s) 825, 1305
BceAI ACGGC 1 cut(s) 1491
BciT130I CCWGG 2 cut(s) 1060, 1080
BclI TGATCA 2 cut(s) 549, 964
BfaI CTAG 3 cut(s) 1026, 1322, 1481
BfrI CTTAAG 1 cut(s) 656
BfuAI ACCTGC 1 cut(s) 928
BisI GCNGC 3 cut(s) 1035, 1509, 1582
BlsI GCNGC 3 cut(s) 1036, 1510, 1583
Bme1390I CCNGG 2 cut(s) 1060, 1080
BmeRI GACNNNNNGTC 1 cut(s) 139
BmeT110I CYCGRG 1 cut(s) 32
BmiI GGNNCC 3 cut(s) 918, 1008, 1294
BmrFI CCNGG 2 cut(s) 1060, 1080
BmrI ACTGGG 2 cut(s) 1324, 1604
BmsI GCATC 5 cut(s) 184, 357, 984, 1335, 1630
BmuI ACTGGG 2 cut(s) 1324, 1604
BpiI GAAGAC 2 cut(s) 138, 452
Bpu10I CCTNAGC 1 cut(s) 338
BpuEI CTTGAG 4 cut(s) 494, 879, 1228, 1642
BsaHI GRCGYC 1 cut(s) 992
BsaJI CCNNGG 2 cut(s) 1059, 1079
BsaXI ACNNNNNCTCC 2 cut(s) 449, 479
Bsc4I CCNNNNNNNGG 3 cut(s) 100, 694, 1289
Bse1I ACTGG 3 cut(s) 732, 1330, 1610
Bse3DI GCAATG 1 cut(s) 204
BseBI CCWGG 2 cut(s) 1060, 1080
BseDI CCNNGG 2 cut(s) 1059, 1079
BseGI GGATG 1 cut(s) 1069
BseLI CCNNNNNNNGG 3 cut(s) 100, 694, 1289
BseMI GCAATG 1 cut(s) 204
BseMII CTCAG 2 cut(s) 1350, 1478
BseNI ACTGG 3 cut(s) 732, 1330, 1610
BseRI GAGGAG 4 cut(s) 50, 113, 218, 344
BseXI GCAGC 3 cut(s) 1046, 1520, 1593
BseYI CCCAGC 1 cut(s) 1159
Bsh1236I CGCG 1 cut(s) 96
BshFI GGCC 3 cut(s) 605, 857, 1201
BsiHKCI CYCGRG 1 cut(s) 32
BslFI GGGAC 1 cut(s) 603
BslI CCNNNNNNNGG 3 cut(s) 100, 694, 1289
BsmFI GGGAC 1 cut(s) 603
BsnI GGCC 3 cut(s) 605, 857, 1201
BsoBI CYCGRG 1 cut(s) 32
Bsp143I GATC 6 cut(s) 90, 549, 964, 1292, 1549, 1602
BspACI CCGC 3 cut(s) 94, 313, 1283
BspANI GGCC 3 cut(s) 605, 857, 1201
BspCNI CTCAG 2 cut(s) 1351, 1477
BspFNI CGCG 1 cut(s) 96
BspLI GGNNCC 3 cut(s) 918, 1008, 1294
BspMI ACCTGC 1 cut(s) 928
BspPI GGATC 5 cut(s) 85, 1287, 1300, 1557, 1597
BspTI CTTAAG 1 cut(s) 656
BsrDI GCAATG 1 cut(s) 204
BsrI ACTGG 3 cut(s) 732, 1330, 1610
BssECI CCNNGG 2 cut(s) 1059, 1079
BssMI GATC 6 cut(s) 90, 549, 964, 1292, 1549, 1602
BssNI GRCGYC 1 cut(s) 992
Bst2UI CCWGG 2 cut(s) 1060, 1080
Bst4CI ACNGT 1 cut(s) 377
Bst6I CTCTTC 2 cut(s) 378, 780
BstACI GRCGYC 1 cut(s) 992
BstAFI CTTAAG 1 cut(s) 656
BstC8I GCNNGC 3 cut(s) 846, 855, 1184
BstDEI CTNAG 5 cut(s) 338, 906, 1359, 1464, 1512
BstENI CCTNNNNNAGG 1 cut(s) 692
BstF5I GGATG 1 cut(s) 1069
BstFNI CGCG 1 cut(s) 96
BstKTI GATC 6 cut(s) 93, 552, 967, 1295, 1552, 1605
BstMBI GATC 6 cut(s) 90, 549, 964, 1292, 1549, 1602
BstMWI GCNNNNNNNGC 4 cut(s) 644, 850, 854, 1084
BstNI CCWGG 2 cut(s) 1060, 1080
BstNSI RCATGY 2 cut(s) 1186, 1289
BstSCI CCNGG 2 cut(s) 1058, 1078
BstUI CGCG 1 cut(s) 96
BstV1I GCAGC 3 cut(s) 1046, 1520, 1593
BstV2I GAAGAC 2 cut(s) 138, 452
BstX2I RGATCY 3 cut(s) 90, 1292, 1602
BstYI RGATCY 3 cut(s) 90, 1292, 1602
BsuRI GGCC 3 cut(s) 605, 857, 1201
BtsCI GGATG 1 cut(s) 1069
BtsIMutI CAGTG 3 cut(s) 725, 1337, 1374
BveI ACCTGC 1 cut(s) 928
Cac8I GCNNGC 3 cut(s) 846, 855, 1184
Csp6I GTAC 4 cut(s) 28, 1118, 1151, 1613
CviAII CATG 4 cut(s) 547, 1183, 1286, 1499
CviQI GTAC 4 cut(s) 28, 1118, 1151, 1613
DdeI CTNAG 5 cut(s) 338, 906, 1359, 1464, 1512
DpnI GATC 6 cut(s) 92, 551, 966, 1294, 1551, 1604
DpnII GATC 6 cut(s) 90, 549, 964, 1292, 1549, 1602
DraI TTTAAA 1 cut(s) 483
DriI GACNNNNNGTC 1 cut(s) 139
Eam1104I CTCTTC 2 cut(s) 378, 780
Eam1105I GACNNNNNGTC 1 cut(s) 139
EarI CTCTTC 2 cut(s) 378, 780
Eco32I GATATC 2 cut(s) 436, 1319
Eco57I CTGAAG 2 cut(s) 71, 809
Eco88I CYCGRG 1 cut(s) 32
EcoNI CCTNNNNNAGG 1 cut(s) 692
EcoRI GAATTC 1 cut(s) 1363
EcoRII CCWGG 2 cut(s) 1058, 1078
EcoRV GATATC 2 cut(s) 436, 1319
FaeI CATG 4 cut(s) 550, 1186, 1289, 1502
FaqI GGGAC 1 cut(s) 603
FatI CATG 4 cut(s) 546, 1182, 1285, 1498
FauI CCCGC 1 cut(s) 306
FbaI TGATCA 2 cut(s) 549, 964
Fnu4HI GCNGC 3 cut(s) 1035, 1509, 1582
FokI GGATG 1 cut(s) 1076
Fsp4HI GCNGC 3 cut(s) 1035, 1509, 1582
FspBI CTAG 3 cut(s) 1026, 1322, 1481
GluI GCNGC 3 cut(s) 1035, 1509, 1582
GsaI CCCAGC 1 cut(s) 1163
HaeIII GGCC 3 cut(s) 605, 857, 1201
Hin1I GRCGYC 1 cut(s) 992
Hin1II CATG 4 cut(s) 550, 1186, 1289, 1502
HincII GTYRAC 1 cut(s) 1593
HindII GTYRAC 1 cut(s) 1593
HindIII AAGCTT 2 cut(s) 842, 1445
HinfI GANTC 7 cut(s) 5, 75, 140, 617, 722, 1013, 1534
HpaI GTTAAC 1 cut(s) 1593
HphI GGTGA 3 cut(s) 1011, 1272, 1468
Hpy166II GTNNAC 3 cut(s) 767, 780, 1593
Hpy188I TCNGA 8 cut(s) 90, 145, 828, 1302, 1467, 1494, 1533, 1539
Hpy188III TCNNGA 5 cut(s) 158, 1242, 1481, 1621, 1684
Hpy8I GTNNAC 3 cut(s) 767, 780, 1593
HpyAV CCTTC 5 cut(s) 384, 494, 960, 1207, 1255
HpyCH4III ACNGT 1 cut(s) 377
HpyCH4IV ACGT 2 cut(s) 154, 992
HpyF10VI GCNNNNNNNGC 4 cut(s) 644, 850, 854, 1084
HpyF3I CTNAG 5 cut(s) 338, 906, 1359, 1464, 1512
HpySE526I ACGT 2 cut(s) 154, 992
Hsp92I GRCGYC 1 cut(s) 992
Hsp92II CATG 4 cut(s) 550, 1186, 1289, 1502
Ksp22I TGATCA 2 cut(s) 549, 964
KspAI GTTAAC 1 cut(s) 1593
Kzo9I GATC 6 cut(s) 90, 549, 964, 1292, 1549, 1602
LmnI GCTCC 2 cut(s) 37, 1264
Lsp1109I GCAGC 3 cut(s) 1046, 1520, 1593
LweI GCATC 5 cut(s) 184, 357, 984, 1335, 1630
MaeI CTAG 3 cut(s) 1026, 1322, 1481
MaeII ACGT 2 cut(s) 154, 992
MaeIII GTNAC 6 cut(s) 63, 110, 225, 988, 1124, 1239
MalI GATC 6 cut(s) 92, 551, 966, 1294, 1551, 1604
MboI GATC 6 cut(s) 90, 549, 964, 1292, 1549, 1602
MfeI CAATTG 1 cut(s) 960
MflI RGATCY 3 cut(s) 90, 1292, 1602
MlyI GAGTC 2 cut(s) 69, 149
MmeI TCCRAC 4 cut(s) 129, 1020, 1270, 1392
MroXI GAANNNNTTC 2 cut(s) 528, 1249
MseI TTAA 6 cut(s) 482, 657, 678, 740, 1592, 1690
MspCI CTTAAG 1 cut(s) 656
MspR9I CCNGG 2 cut(s) 1060, 1080
MunI CAATTG 1 cut(s) 960
MvaI CCWGG 2 cut(s) 1060, 1080
MvnI CGCG 1 cut(s) 96
MwoI GCNNNNNNNGC 4 cut(s) 644, 850, 854, 1084
NdeII GATC 6 cut(s) 90, 549, 964, 1292, 1549, 1602
NlaIII CATG 4 cut(s) 550, 1186, 1289, 1502
NlaIV GGNNCC 3 cut(s) 918, 1008, 1294
NmuCI GTSAC 3 cut(s) 988, 1124, 1239
NspI RCATGY 2 cut(s) 1186, 1289
PaeI GCATGC 1 cut(s) 1186
PaeR7I CTCGAG 1 cut(s) 32
PdmI GAANNNNTTC 2 cut(s) 528, 1249
PfeI GAWTC 4 cut(s) 617, 722, 1013, 1534
PkrI GCNGC 3 cut(s) 1036, 1510, 1583
PleI GAGTC 2 cut(s) 69, 148
PpsI GAGTC 2 cut(s) 69, 148
PsiI TTATAA 2 cut(s) 543, 1397
Psp6I CCWGG 2 cut(s) 1058, 1078
PspFI CCCAGC 1 cut(s) 1159
PspGI CCWGG 2 cut(s) 1058, 1078
PspN4I GGNNCC 3 cut(s) 918, 1008, 1294
PspXI VCTCGAGB 1 cut(s) 32
PsuI RGATCY 3 cut(s) 90, 1292, 1602
RsaI GTAC 4 cut(s) 29, 1119, 1152, 1614
RsaNI GTAC 4 cut(s) 28, 1118, 1151, 1613
SaqAI TTAA 6 cut(s) 482, 657, 678, 740, 1592, 1690
SatI GCNGC 3 cut(s) 1035, 1509, 1582
Sau3AI GATC 6 cut(s) 90, 549, 964, 1292, 1549, 1602
SchI GAGTC 2 cut(s) 69, 149
ScrFI CCNGG 2 cut(s) 1060, 1080
SfaNI GCATC 5 cut(s) 184, 357, 984, 1335, 1630
Sfr274I CTCGAG 1 cut(s) 32
SlaI CTCGAG 1 cut(s) 32
SmlI CTYRAG 6 cut(s) 32, 509, 656, 894, 1207, 1621
SmoI CTYRAG 6 cut(s) 32, 509, 656, 894, 1207, 1621
SphI GCATGC 1 cut(s) 1186
SsiI CCGC 3 cut(s) 94, 313, 1283
SspI AATATT 1 cut(s) 1662
SspMI CTAG 3 cut(s) 1026, 1322, 1481
StyD4I CCNGG 2 cut(s) 1058, 1078
TaaI ACNGT 1 cut(s) 377
TaiI ACGT 2 cut(s) 157, 995
TaqI TCGA 3 cut(s) 33, 381, 1552
TaqII GACCGA 1 cut(s) 1134
TatI WGTACW 1 cut(s) 1117
TfiI GAWTC 4 cut(s) 617, 722, 1013, 1534
Tru1I TTAA 6 cut(s) 482, 657, 678, 740, 1592, 1690
Tru9I TTAA 6 cut(s) 482, 657, 678, 740, 1592, 1690
TscAI CASTG 3 cut(s) 732, 1337, 1374
TseFI GTSAC 3 cut(s) 988, 1124, 1239
TseI GCWGC 3 cut(s) 1034, 1508, 1581
Tsp45I GTSAC 3 cut(s) 988, 1124, 1239
TspDTI ATGAA 4 cut(s) 582, 666, 845, 942
TspRI CASTG 3 cut(s) 732, 1337, 1374
Vha464I CTTAAG 1 cut(s) 656
XagI CCTNNNNNAGG 1 cut(s) 692
XapI RAATTY 3 cut(s) 1096, 1189, 1363
XbaI TCTAGA 1 cut(s) 1480
XceI RCATGY 2 cut(s) 1186, 1289
XcmI CCANNNNNNNNNTGG 1 cut(s) 268
XhoI CTCGAG 1 cut(s) 32
XmnI GAANNNNTTC 2 cut(s) 528, 1249
XspI CTAG 3 cut(s) 1026, 1322, 1481
ZraI GACGTC 1 cut(s) 993
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.