Rmu_sc0025540.1_g000001

ABIL2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0025540.1
OG0007509
Physical Location & Seq
Forward (+)
1 .. 1015
1015 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0025540.1_g000001.1.cds

Sequence Viewer

Length: 500 bp
ttacaaaagtaaagggtgacattttgtgttcaggtggagcaaccactcatactgttggccagtctgcattgatatatcatagtgctcaaggggacttgtctgggactaatactgcaacaacaagggagacctctccaacagttgtcagtgaagagtattctaatatgcattctccagaatcttcacctggagcatttcagttcacaagaatcggatccaaacatgagaaacgaacagtctcaccacttaggtttccaattccacggtgcggctctgttaagagatcaagcactctgaactcttcttctagtgccaaactgcggtacccatcggagcctcggactaggagatcagcttcgttatccctttataatgaaagagacaggactaaagacattgaagtggattcaagcaaaaataaacgattctacaaggccttactgagcatgggcaaatctagaaatgatactgcattgtacaaatatttggataagaactaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

165

Amino Acids

18.21

Weight (kDa)

9.92

Isoelectric Point (pI)

62.25

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 371
Acc65I GGTACC 1 cut(s) 323
AccB1I GGYRCC 1 cut(s) 323
AciI CCGC 2 cut(s) 269, 321
AclWI GGATC 2 cut(s) 209, 222
AcoI YGGCCR 1 cut(s) 57
AfaI GTAC 2 cut(s) 325, 478
AfiI CCNNNNNNNGG 2 cut(s) 268, 320
AgsI TTSAA 2 cut(s) 400, 410
AjnI CCWGG 1 cut(s) 186
AluBI AGCT 1 cut(s) 355
AluI AGCT 1 cut(s) 355
Alw21I GWGCWC 1 cut(s) 87
Alw26I GTCTC 3 cut(s) 121, 243, 374
AlwI GGATC 2 cut(s) 209, 222
AoxI GGCC 2 cut(s) 57, 434
Asp718I GGTACC 1 cut(s) 323
AsuHPI GGTGA 3 cut(s) 28, 176, 233
BaeI ACNNNNGTAYC 2 cut(s) 307, 340
BalI TGGCCA 1 cut(s) 59
BamHI GGATCC 1 cut(s) 214
BanI GGYRCC 1 cut(s) 323
Bbv12I GWGCWC 1 cut(s) 87
BccI CCATC 1 cut(s) 336
BciT130I CCWGG 1 cut(s) 188
BcoDI GTCTC 3 cut(s) 121, 243, 374
BfaI CTAG 3 cut(s) 308, 344, 458
BisI GCNGC 1 cut(s) 270
BlsI GCNGC 1 cut(s) 271
Bme1390I CCNGG 1 cut(s) 188
BmiI GGNNCC 3 cut(s) 216, 325, 335
BmrFI CCNGG 1 cut(s) 188
BpmI CTGGAG 2 cut(s) 158, 209
BpuEI CTTGAG 1 cut(s) 71
BsaI GGTCTC 1 cut(s) 121
BsaJI CCNNGG 2 cut(s) 262, 337
Bsc4I CCNNNNNNNGG 2 cut(s) 268, 320
Bse1I ACTGG 1 cut(s) 60
BseBI CCWGG 1 cut(s) 188
BseDI CCNNGG 2 cut(s) 262, 337
BseLI CCNNNNNNNGG 2 cut(s) 268, 320
BseMII CTCAG 1 cut(s) 433
BseNI ACTGG 1 cut(s) 60
BshFI GGCC 2 cut(s) 59, 436
BshNI GGYRCC 1 cut(s) 323
BsiHKAI GWGCWC 1 cut(s) 87
BslFI GGGAC 2 cut(s) 106, 117
BslI CCNNNNNNNGG 2 cut(s) 268, 320
BsmAI GTCTC 3 cut(s) 121, 243, 374
BsmFI GGGAC 2 cut(s) 106, 117
BsmI GAATGC 1 cut(s) 168
BsnI GGCC 2 cut(s) 59, 436
Bso31I GGTCTC 1 cut(s) 121
Bsp1286I GDGCHC 1 cut(s) 87
Bsp1407I TGTACA 1 cut(s) 476
Bsp143I GATC 3 cut(s) 214, 283, 349
BspACI CCGC 2 cut(s) 269, 321
BspANI GGCC 2 cut(s) 59, 436
BspCNI CTCAG 1 cut(s) 434
BspLI GGNNCC 3 cut(s) 216, 325, 335
BspPI GGATC 2 cut(s) 209, 222
BspT107I GGYRCC 1 cut(s) 323
BspTNI GGTCTC 1 cut(s) 121
BsrGI TGTACA 1 cut(s) 476
BsrI ACTGG 1 cut(s) 60
BssECI CCNNGG 2 cut(s) 262, 337
BssMI GATC 3 cut(s) 214, 283, 349
Bst2UI CCWGG 1 cut(s) 188
Bst4CI ACNGT 4 cut(s) 54, 141, 237, 266
Bst6I CTCTTC 2 cut(s) 146, 306
BstAUI TGTACA 1 cut(s) 476
BstDEI CTNAG 2 cut(s) 247, 442
BstDSI CCRYGG 1 cut(s) 262
BstKTI GATC 3 cut(s) 217, 286, 352
BstMAI GTCTC 3 cut(s) 121, 243, 374
BstMBI GATC 3 cut(s) 214, 283, 349
BstNI CCWGG 1 cut(s) 188
BstSCI CCNGG 1 cut(s) 186
BstX2I RGATCY 1 cut(s) 214
BstYI RGATCY 1 cut(s) 214
BsuRI GGCC 2 cut(s) 59, 436
BtgI CCRYGG 1 cut(s) 262
BtsIMutI CAGTG 1 cut(s) 153
Csp6I GTAC 2 cut(s) 324, 477
CviAII CATG 2 cut(s) 223, 447
CviJI RGCY 5 cut(s) 59, 272, 336, 355, 436
CviKI_1 RGCY 5 cut(s) 59, 272, 336, 355, 436
CviQI GTAC 2 cut(s) 324, 477
DdeI CTNAG 2 cut(s) 247, 442
DpnI GATC 3 cut(s) 216, 285, 351
DpnII GATC 3 cut(s) 214, 283, 349
EaeI YGGCCR 1 cut(s) 57
Eam1104I CTCTTC 2 cut(s) 146, 306
EarI CTCTTC 2 cut(s) 146, 306
Eco147I AGGCCT 1 cut(s) 436
Eco31I GGTCTC 1 cut(s) 121
EcoRII CCWGG 1 cut(s) 186
EcoT22I ATGCAT 1 cut(s) 170
FaeI CATG 2 cut(s) 226, 450
FaiI YATR 7 cut(s) 50, 75, 80, 166, 224, 371, 448
FaqI GGGAC 2 cut(s) 106, 117
FatI CATG 2 cut(s) 222, 446
Fnu4HI GCNGC 1 cut(s) 270
Fsp4HI GCNGC 1 cut(s) 270
FspBI CTAG 3 cut(s) 308, 344, 458
GluI GCNGC 1 cut(s) 270
GsuI CTGGAG 2 cut(s) 158, 209
HaeIII GGCC 2 cut(s) 59, 436
Hin1II CATG 2 cut(s) 226, 450
HinfI GANTC 4 cut(s) 178, 209, 406, 425
HphI GGTGA 3 cut(s) 28, 176, 233
Hpy166II GTNNAC 1 cut(s) 203
Hpy188I TCNGA 4 cut(s) 214, 296, 333, 341
Hpy188III TCNNGA 2 cut(s) 175, 458
Hpy8I GTNNAC 1 cut(s) 203
HpyCH4III ACNGT 4 cut(s) 54, 141, 237, 266
HpyCH4V TGCA 4 cut(s) 67, 115, 168, 472
HpyF3I CTNAG 2 cut(s) 247, 442
Hsp92II CATG 2 cut(s) 226, 450
KpnI GGTACC 1 cut(s) 327
Kzo9I GATC 3 cut(s) 214, 283, 349
LmnI GCTCC 3 cut(s) 37, 190, 333
LpnPI CCDG 7 cut(s) 17, 73, 86, 173, 188, 200, 369
MaeI CTAG 3 cut(s) 308, 344, 458
MaeIII GTNAC 1 cut(s) 16
MalI GATC 3 cut(s) 216, 285, 351
MboI GATC 3 cut(s) 214, 283, 349
MboII GAAGA 4 cut(s) 163, 173, 293, 296
MflI RGATCY 1 cut(s) 214
MhlI GDGCHC 1 cut(s) 87
MlsI TGGCCA 1 cut(s) 59
MluCI AATT 1 cut(s) 257
MluNI TGGCCA 1 cut(s) 59
MmeI TCCRAC 1 cut(s) 160
MnlI CCTC 2 cut(s) 141, 347
Mox20I TGGCCA 1 cut(s) 59
Mph1103I ATGCAT 1 cut(s) 170
MscI TGGCCA 1 cut(s) 59
MseI TTAA 1 cut(s) 278
MslI CAYNNNNRTG 1 cut(s) 400
Msp20I TGGCCA 1 cut(s) 59
MspR9I CCNGG 1 cut(s) 188
Mva1269I GAATGC 1 cut(s) 168
MvaI CCWGG 1 cut(s) 188
NdeII GATC 3 cut(s) 214, 283, 349
NlaIII CATG 2 cut(s) 226, 450
NlaIV GGNNCC 3 cut(s) 216, 325, 335
NmuCI GTSAC 1 cut(s) 16
NsiI ATGCAT 1 cut(s) 170
PceI AGGCCT 1 cut(s) 436
PctI GAATGC 1 cut(s) 168
PfeI GAWTC 4 cut(s) 178, 209, 406, 425
PkrI GCNGC 1 cut(s) 271
PsiI TTATAA 1 cut(s) 371
Psp6I CCWGG 1 cut(s) 186
PspGI CCWGG 1 cut(s) 186
PspN4I GGNNCC 3 cut(s) 216, 325, 335
PsuI RGATCY 1 cut(s) 214
RsaI GTAC 2 cut(s) 325, 478
RsaNI GTAC 2 cut(s) 324, 477
RseI CAYNNNNRTG 1 cut(s) 400
SaqAI TTAA 1 cut(s) 278
SatI GCNGC 1 cut(s) 270
Sau3AI GATC 3 cut(s) 214, 283, 349
ScrFI CCNGG 1 cut(s) 188
SduI GDGCHC 1 cut(s) 87
SetI ASST 5 cut(s) 36, 133, 189, 253, 357
SmiMI CAYNNNNRTG 1 cut(s) 400
SmlI CTYRAG 1 cut(s) 86
SmoI CTYRAG 1 cut(s) 86
Sse9I AATT 1 cut(s) 257
SseBI AGGCCT 1 cut(s) 436
SsiI CCGC 2 cut(s) 269, 321
SspI AATATT 1 cut(s) 484
SspMI CTAG 3 cut(s) 308, 344, 458
StuI AGGCCT 1 cut(s) 436
StyD4I CCNGG 1 cut(s) 186
TaaI ACNGT 4 cut(s) 54, 141, 237, 266
TasI AATT 1 cut(s) 257
TatI WGTACW 1 cut(s) 476
TauI GCSGC 1 cut(s) 272
TfiI GAWTC 4 cut(s) 178, 209, 406, 425
Tru1I TTAA 1 cut(s) 278
Tru9I TTAA 1 cut(s) 278
TscAI CASTG 1 cut(s) 153
TseFI GTSAC 1 cut(s) 16
Tsp45I GTSAC 1 cut(s) 16
TspDTI ATGAA 1 cut(s) 389
TspRI CASTG 1 cut(s) 153
XbaI TCTAGA 1 cut(s) 457
XspI CTAG 3 cut(s) 308, 344, 458
Zsp2I ATGCAT 1 cut(s) 170
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.